Rh4DG028100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000383
Physical Location & Seq
Reverse (-)
4994494 .. 4996169
1676 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG028100.1

Sequence Viewer

Length: 831 bp
ATGAAAGAGACCAACGTCGTTCAGGCGAACAGCACGGAGACTAACGTCATTCCGACCACTAACACAGAAACCTACGGCGTTTCGGCGACCAATACAGAGGTCAATGTCATTCCGGCGACCACGGAGACCAACGCCCAAGCTCTCTGCTTCAAATTCTCTTCGGTCGAAAATGAGTCCAAGTCTACTACTCGTTCCCCTTGGGTTGCGGGGACAGCTTACTTAGACGGCTCAAGAGAATGGATGTCAGGCCCGTCTACTTTTCTCTCAGCCGAGTTTCAGCCTGAGCAAGAAGGCAGTCCACCTGCATATCGTTGGTGGGCGGATCGCGATTATCTCGGAAACAAATACGCTGAGATTGGTAACGGGGAATCATACTCGGGAGTACCGAGGGTGCTGGAATTGAGTAACGGCGACGCAGTATCACGCTTTGCCCTTGCTCCCGAATTCGTCCCTTCTTCTTCAACTGCCTGTAACCAGCCCCAGCACCAGCGTCAACCTCCAAAGCGTCCTTCACCGCTCTACAAGACGGAGTTTTGTCGGAAAACATCTTGCTCGAAGGGAGACCTGTGTGACTTTGCCCATAGTGTTGACGAGATTCGCATGCGACCCAAGTTCCCCTCAAAGTGCAAATGGATGGATGGCCTTTGGGGCACAAAGGCAAATACCTTTGTTTTTGCCACCCTACAGTTTAACTCCATCAGACAGAGGACTGGATGTTTCTTTTACGTGCATCACCATAAAAGGATTAAAAGCATTACCTTTGTAGTTTTTGGCCAAGCATTATCTTTTATAGTATTTGGCCAGATTGGAGAGCTTGATCGGCCTCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

276

Amino Acids

30.93

Weight (kDa)

8.43

Isoelectric Point (pI)

47.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000383)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07180 AT5G07180 AT5G62230 AT5G62230
fragaria_vesca FvH4_7g28290 FvH4_7g28290 FvH4_7g28290 FvH4_7g28290
malus_domestica MD02G1216900.v1.1 MD07G1259500.v1.1 MD17G1114200.v1.1 MD17G1114300.v1.1
prunus_persica Prupe.2G283600_v2.0.a1
pyrus_communis pycom05g04060 pycom07g23280 pycom15g04040
rosa_chinensis RchiOBHm_Chr1g0375261 RchiOBHm_Chr2g0167851 RchiOBHm_Chr4g0390421 RchiOBHm_Chr4g0390431 RchiOBHm_Chr4g0442901 RchiOBHm_Chr4g0442981 RchiOBHm_Chr7g0228991 RchiOBHm_Chr7g0239711
rosa_laevigata RLG00000005460 RLG00000005968 RLG00000005971 RLG00000005973 RLG00000005978 RLG00000008547 RLG00000010012 RLG00000010245 RLG00000026673 RLG00000030046 RLG00000031637
rosa_multiflora Rmu_co8166860.1_g000001 Rmu_co8410161.1_g000001 Rmu_sc0000554.1_g000034 Rmu_sc0001782.1_g000050 Rmu_sc0002096.1_g000036 Rmu_sc0003006.1_g000022 Rmu_sc0003701.1_g000003 Rmu_sc0005877.1_g000004 Rmu_sc0006329.1_g000003 Rmu_sc0006695.1_g000032 Rmu_sc0008108.1_g000003 Rmu_sc0009414.1_g000003 Rmu_sc0010356.1_g000001 Rmu_sc0014119.1_g000002 Rmu_sc0014119.1_g000003 Rmu_sc0014161.1_g000004 Rmu_sc0023009.1_g000001 Rmu_sc0036835.1_g000001 Rmu_ssc0000204.1_g000009
rosa_roxburghii Rroxscaffold_1G00024810 Rroxscaffold_2G00110800 Rroxscaffold_2G00122430 Rroxscaffold_3G00261280 Rroxscaffold_4G00282890 Rroxscaffold_4G00330910 Rroxscaffold_5G00370350 Rroxscaffold_7G00161820
rosa_rugosa Rorug01G0389600 Rorug01G0389700 Rorug01G0389800 Rorug01G0389900 Rorug01G0389900 Rorug01G0390000 Rorug04G0343700 Rorug07G0155300
rosa_samantha Rh1AG400300 Rh1CG377300 Rh1DG394700 Rh2AG367100 Rh2BG612000 Rh2CG350500 Rh2DG389600 Rh3CG058100 Rh4AG396700 Rh4AG397100 Rh4AG397500 Rh4AG397600 Rh4AG397700 Rh4BG003000 Rh4BG127700 Rh4BG370500 Rh4BG393400 Rh4BG409200 Rh4CG035400 Rh4CG166700 Rh4CG408200 Rh4CG424700 Rh4DG028000 Rh4DG028100 Rh4DG403300 Rh4DG403800 Rh6DG080900 Rh7DG202700
rosa_wichuraiana Rw1G035540 Rw4G032760 Rw4G034230 Rw4G034240 Rw4G034260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 310
Acc36I ACCTGC 1 cut(s) 310
AccBSI CCGCTC 1 cut(s) 517
AccI GTMKAC 2 cut(s) 182, 254
AccII CGCG 1 cut(s) 327
AciI CCGC 3 cut(s) 206, 320, 515
AclWI GGATC 1 cut(s) 330
AcoI YGGCCR 2 cut(s) 772, 799
AcsI RAATTY 2 cut(s) 152, 443
AfaI GTAC 1 cut(s) 384
AgsI TTSAA 2 cut(s) 151, 462
AjuI GAANNNNNNNTTGG 2 cut(s) 493, 525
AluBI AGCT 3 cut(s) 140, 215, 814
AluI AGCT 3 cut(s) 140, 215, 814
Alw26I GTCTC 4 cut(s) 2, 32, 119, 555
AlwI GGATC 1 cut(s) 330
Ama87I CYCGRG 1 cut(s) 376
AoxI GGCC 5 cut(s) 247, 640, 772, 799, 821
ApoI RAATTY 2 cut(s) 152, 443
AspS9I GGNCC 1 cut(s) 248
AsuHPI GGTGA 2 cut(s) 504, 725
AvaI CYCGRG 1 cut(s) 376
BaeGI GKGCMC 1 cut(s) 653
BalI TGGCCA 2 cut(s) 774, 801
BccI CCATC 3 cut(s) 628, 632, 704
BceAI ACGGC 3 cut(s) 91, 241, 424
BcoDI GTCTC 4 cut(s) 2, 32, 119, 555
BfmI CTRYAG 1 cut(s) 683
BfuAI ACCTGC 1 cut(s) 310
BglI GCCNNNNNGGC 1 cut(s) 648
BmeT110I CYCGRG 1 cut(s) 376
BmgT120I GGNCC 1 cut(s) 248
BmsI GCATC 1 cut(s) 739
BoxI GACNNNNGTC 2 cut(s) 14, 44
Bpu10I CCTNAGC 1 cut(s) 282
BpuEI CTTGAG 1 cut(s) 214
BsaAI YACGTR 1 cut(s) 727
BsaI GGTCTC 3 cut(s) 2, 119, 555
BsaJI CCNNGG 3 cut(s) 120, 197, 386
Bse1I ACTGG 1 cut(s) 715
BseDI CCNNGG 3 cut(s) 120, 197, 386
BseGI GGATG 4 cut(s) 246, 639, 643, 719
BseMII CTCAG 3 cut(s) 273, 279, 342
BseNI ACTGG 1 cut(s) 715
BseSI GKGCMC 1 cut(s) 653
BseYI CCCAGC 1 cut(s) 480
Bsh1236I CGCG 1 cut(s) 327
Bsh1285I CGRYCG 1 cut(s) 165
BshFI GGCC 5 cut(s) 249, 642, 774, 801, 823
BsiEI CGRYCG 1 cut(s) 165
BsiHKCI CYCGRG 1 cut(s) 376
BsiSI CCGG 1 cut(s) 113
BslFI GGGAC 2 cut(s) 223, 434
BsmAI GTCTC 4 cut(s) 2, 32, 119, 555
BsmFI GGGAC 2 cut(s) 223, 434
BsnI GGCC 5 cut(s) 249, 642, 774, 801, 823
Bso31I GGTCTC 3 cut(s) 2, 119, 555
BsoBI CYCGRG 1 cut(s) 376
Bsp1286I GDGCHC 1 cut(s) 653
Bsp143I GATC 2 cut(s) 322, 817
Bsp68I TCGCGA 1 cut(s) 327
BspACI CCGC 3 cut(s) 206, 320, 515
BspANI GGCC 5 cut(s) 249, 642, 774, 801, 823
BspCNI CTCAG 3 cut(s) 274, 278, 343
BspFNI CGCG 1 cut(s) 327
BspMI ACCTGC 1 cut(s) 310
BspPI GGATC 1 cut(s) 330
BspTNI GGTCTC 3 cut(s) 2, 119, 555
BsrBI CCGCTC 1 cut(s) 517
BsrI ACTGG 1 cut(s) 715
BssECI CCNNGG 3 cut(s) 120, 197, 386
BssMI GATC 2 cut(s) 322, 817
BssT1I CCWWGG 1 cut(s) 197
Bst4CI ACNGT 1 cut(s) 687
Bst6I CTCTTC 1 cut(s) 163
BstBAI YACGTR 1 cut(s) 727
BstC8I GCNNGC 1 cut(s) 602
BstDEI CTNAG 4 cut(s) 220, 265, 282, 351
BstDSI CCRYGG 1 cut(s) 120
BstF5I GGATG 4 cut(s) 246, 639, 643, 719
BstFNI CGCG 1 cut(s) 327
BstKTI GATC 2 cut(s) 325, 820
BstMAI GTCTC 4 cut(s) 2, 32, 119, 555
BstMBI GATC 2 cut(s) 322, 817
BstMCI CGRYCG 1 cut(s) 165
BstMWI GCNNNNNNNGC 3 cut(s) 212, 648, 820
BstNSI RCATGY 1 cut(s) 604
BstPAI GACNNNNGTC 2 cut(s) 14, 44
BstSFI CTRYAG 1 cut(s) 683
BstSLI GKGCMC 1 cut(s) 653
BstUI CGCG 1 cut(s) 327
BsuRI GGCC 5 cut(s) 249, 642, 774, 801, 823
BtgI CCRYGG 1 cut(s) 120
BtsCI GGATG 4 cut(s) 246, 639, 643, 719
BtuMI TCGCGA 1 cut(s) 327
BveI ACCTGC 1 cut(s) 310
Cac8I GCNNGC 1 cut(s) 602
Cfr13I GGNCC 1 cut(s) 248
CseI GACGC 3 cut(s) 422, 479, 494
Csp6I GTAC 1 cut(s) 383
CviAII CATG 1 cut(s) 601
CviQI GTAC 1 cut(s) 383
DdeI CTNAG 4 cut(s) 220, 265, 282, 351
DpnI GATC 2 cut(s) 324, 819
DpnII GATC 2 cut(s) 322, 817
EaeI YGGCCR 2 cut(s) 772, 799
Eam1104I CTCTTC 1 cut(s) 163
EarI CTCTTC 1 cut(s) 163
EciI GGCGGA 1 cut(s) 335
Eco130I CCWWGG 1 cut(s) 197
Eco31I GGTCTC 3 cut(s) 2, 119, 555
Eco88I CYCGRG 1 cut(s) 376
EcoRI GAATTC 1 cut(s) 443
EcoT14I CCWWGG 1 cut(s) 197
ErhI CCWWGG 1 cut(s) 197
FaeI CATG 1 cut(s) 604
FaiI YATR 6 cut(s) 307, 373, 582, 602, 738, 791
FaqI GGGAC 2 cut(s) 223, 434
FatI CATG 1 cut(s) 600
FauI CCCGC 1 cut(s) 199
FblI GTMKAC 2 cut(s) 182, 254
FokI GGATG 4 cut(s) 253, 646, 650, 726
GsaI CCCAGC 1 cut(s) 484
HaeIII GGCC 5 cut(s) 249, 642, 774, 801, 823
HapII CCGG 1 cut(s) 113
HgaI GACGC 3 cut(s) 422, 479, 494
Hin1II CATG 1 cut(s) 604
HincII GTYRAC 2 cut(s) 494, 589
HindII GTYRAC 2 cut(s) 494, 589
HinfI GANTC 3 cut(s) 173, 368, 595
HpaII CCGG 1 cut(s) 113
HphI GGTGA 2 cut(s) 504, 725
Hpy166II GTNNAC 5 cut(s) 183, 255, 299, 494, 589
Hpy188I TCNGA 4 cut(s) 54, 338, 540, 701
Hpy188III TCNNGA 4 cut(s) 231, 326, 378, 440
Hpy8I GTNNAC 5 cut(s) 183, 255, 299, 494, 589
Hpy99I CGWCG 2 cut(s) 20, 416
HpyAV CCTTC 4 cut(s) 284, 462, 519, 550
HpyCH4III ACNGT 1 cut(s) 687
HpyCH4IV ACGT 3 cut(s) 15, 45, 726
HpyCH4V TGCA 3 cut(s) 305, 627, 730
HpyF10VI GCNNNNNNNGC 3 cut(s) 212, 648, 820
HpyF3I CTNAG 4 cut(s) 220, 265, 282, 351
HpySE526I ACGT 3 cut(s) 15, 45, 726
Hsp92II CATG 1 cut(s) 604
Kzo9I GATC 2 cut(s) 322, 817
LmnI GCTCC 1 cut(s) 442
LweI GCATC 1 cut(s) 739
MaeII ACGT 3 cut(s) 15, 45, 726
MaeIII GTNAC 4 cut(s) 359, 404, 470, 569
MalI GATC 2 cut(s) 324, 819
MbiI CCGCTC 1 cut(s) 517
MboI GATC 2 cut(s) 322, 817
MboII GAAGA 3 cut(s) 150, 447, 450
MhlI GDGCHC 1 cut(s) 653
MlsI TGGCCA 2 cut(s) 774, 801
MluCI AATT 3 cut(s) 152, 398, 443
MluNI TGGCCA 2 cut(s) 774, 801
MlyI GAGTC 1 cut(s) 182
MmeI TCCRAC 2 cut(s) 77, 518
MnlI CCTC 5 cut(s) 91, 381, 507, 628, 699
Mox20I TGGCCA 2 cut(s) 774, 801
MscI TGGCCA 2 cut(s) 774, 801
MseI TTAA 3 cut(s) 690, 747, 829
Msp20I TGGCCA 2 cut(s) 774, 801
MspI CCGG 1 cut(s) 113
MvnI CGCG 1 cut(s) 327
MwoI GCNNNNNNNGC 3 cut(s) 212, 648, 820
NdeII GATC 2 cut(s) 322, 817
NlaIII CATG 1 cut(s) 604
NmeAIII GCCGAG 1 cut(s) 295
NmuCI GTSAC 1 cut(s) 569
NruI TCGCGA 1 cut(s) 327
NspI RCATGY 1 cut(s) 604
PaeI GCATGC 1 cut(s) 604
PaqCI CACCTGC 1 cut(s) 310
PcsI WCGNNNNNNNCGW 1 cut(s) 383
PfeI GAWTC 2 cut(s) 368, 595
PleI GAGTC 1 cut(s) 181
PpsI GAGTC 1 cut(s) 181
Ppu21I YACGTR 1 cut(s) 727
PshAI GACNNNNGTC 2 cut(s) 14, 44
PspFI CCCAGC 1 cut(s) 480
PspPI GGNCC 1 cut(s) 248
RruI TCGCGA 1 cut(s) 327
RsaI GTAC 1 cut(s) 384
RsaNI GTAC 1 cut(s) 383
SaqAI TTAA 3 cut(s) 690, 747, 829
Sau3AI GATC 2 cut(s) 322, 817
Sau96I GGNCC 1 cut(s) 248
SchI GAGTC 1 cut(s) 182
SduI GDGCHC 1 cut(s) 653
SfaNI GCATC 1 cut(s) 739
SfcI CTRYAG 1 cut(s) 683
SmlI CTYRAG 1 cut(s) 229
SmoI CTYRAG 1 cut(s) 229
SphI GCATGC 1 cut(s) 604
Sse9I AATT 3 cut(s) 152, 398, 443
SsiI CCGC 3 cut(s) 206, 320, 515
StyI CCWWGG 1 cut(s) 197
TaaI ACNGT 1 cut(s) 687
TaiI ACGT 3 cut(s) 18, 48, 729
TaqI TCGA 2 cut(s) 165, 554
TaqII GACCGA 1 cut(s) 151
TasI AATT 3 cut(s) 152, 398, 443
TfiI GAWTC 2 cut(s) 368, 595
Tru1I TTAA 3 cut(s) 690, 747, 829
Tru9I TTAA 3 cut(s) 690, 747, 829
TseFI GTSAC 1 cut(s) 569
Tsp45I GTSAC 1 cut(s) 569
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 3 cut(s) 50, 137, 542
XapI RAATTY 2 cut(s) 152, 443
XceI RCATGY 1 cut(s) 604
XmiI GTMKAC 2 cut(s) 182, 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.