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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 3 cut(s) | 44, 149, 161 |
| AgsI | TTSAA | 3 cut(s) | 40, 138, 160 |
| ApoI | RAATTY | 3 cut(s) | 44, 149, 161 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 36, 68 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 44 |
| FaiI | YATR | 2 cut(s) | 102, 175 |
| FspEI | CC | 3 cut(s) | 27, 87, 117 |
| MaeIII | GTNAC | 1 cut(s) | 104 |
| MluCI | AATT | 6 cut(s) | 6, 44, 63, 127, 149, 161 |
| MnlI | CCTC | 1 cut(s) | 24 |
| MroXI | GAANNNNTTC | 1 cut(s) | 44 |
| PdmI | GAANNNNTTC | 1 cut(s) | 44 |
| PsrI | GAACNNNNNNTAC | 6 cut(s) | 54, 86, 97, 118, 129, 150 |
| SetI | ASST | 1 cut(s) | 16 |
| SgeI | CNNG | 2 cut(s) | 44, 156 |
| Sse9I | AATT | 6 cut(s) | 6, 44, 63, 127, 149, 161 |
| TasI | AATT | 6 cut(s) | 6, 44, 63, 127, 149, 161 |
| TspDTI | ATGAA | 1 cut(s) | 111 |
| XapI | RAATTY | 3 cut(s) | 44, 149, 161 |
| XmnI | GAANNNNTTC | 1 cut(s) | 44 |