FvH4_1g20221

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000321
Physical Location & Seq
Reverse (-)
12431379 .. 12438254
6876 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g20221.t4

Sequence Viewer

Length: 2178 bp
ATGGGTTTACCAAAAACCAAGAATGCCTCTACTTCAGAGGAGCCTGATGACACTATTACTGCCTCCACCGGGCTAAAAAGGAAGCGTAGAAGAACAAGTATGCAGGGCACAGTGAACCGAGCACTTAGAGGTAAGAAAGCAGTAGTTGAGTTTAATCCAAAGGGCGTACCGTTTGGCAAGGTAGCATCAGAGATGGCATCTTACATTGGGGTGCTTGCGCGTACCACGGTTCCCATCAGTGTTGAGAGCTGGCCTAAGGTGGATAAGAATCTGAAGAACGAAATTTGGAAATCCGTAGAGATGGCATTCGTCTTAGCGCCTAGACACAAAAAAATTGTTGTGGCATCTGCAGCCAATAAGTGGAGGCAATTCAAGTCAGAATTGACTACAAAATACATATTGCCATACAAAGATCAGCCTGATACTTTAGAAGACCCACCAGAAGAATATGATTTCATTAAGGATCAAGATTGGGTACAATTCGTGAAGAGTCGCTTAACCACAGATTTTCAGAAACTCCATATGGAGCAGAAAGAGAGAAGAGGAAAGTTGCAGAATGCTCATAGAATGTCACGAAAGGGATATGCTAATTTGGAGGCAGAATTAAACGAAAATATGCATGAAGATGAACTTGATGTAGTTGTCCTCCAGAAGAAAGGAGGTCAAGTCAAAAATGGGAATATCTCGCATGAGACTGTAGGGGAGCAGGCTGCAGAATTTGATACGTTGTTGAACAATGAAGGGGCTATTTCCAACTGTAACAATGGATCCAGCGATGACGTTAGGTCAATGGGGTTGGGGACTCCAGAACATTCTGGTAGGGATAGAGGCGAAGGAGAGTCTATCATGCCAAATATTTCACCTCCTCAGTTGGAGCGAGAAGGTATCATGGATGAGGTCAGGAAATTGATAGAGCAGCAAAGGGTATGGTTTGAAGCCAAGATTTCTTTGTTAGAGGCAAGGATCAGTGGTGATTGCCTGGCCACAAGTATGCCACTTTCAACTCCTCTTTTTGCCAAGCCTTCAAAGAAGGGCGGTGGTTCTGGGAAGAACGATGTTGAGGTTAATGAAATTGATTCAGAAGCTTTCTCATTTGGAGGCAGAAAAGAGTTCATGAAGGGAAAATCTTGCAGGCTGGCTGTTGGATTGATTGACAACGTTGTTTCCCATGGTACAATAATTGAAGTGGATGCAGCGAACCACACAGTCCATGGTGTGCCTTTGGGAGAGGGAAATATACGTGTTGCAATTGATACTGCCCTAGATGAACAAGCGCTGCTTCCCATACCAGTTACAGGGGAATTAGTAACTGTTGGGCAAGCTGTAGGCTCTCATGTGGCTTGGCCAAAACGTCTTGTTAAGTTGATGAGTGATGAGGAACTTGGTAGTACTTCTATAAAACCTCGAGATTTTCCCAAAGAAGATGTGAACCTGCCCAAGTCCCTCAAGCTCCTATACCGTTATGCTGAGCGTGCTATGATGGATGGTGAACCTATCAGTGTTTTTGTGGAAGAGGCTGTTTTCGGTATTGCCAAGACTGTCAATATTTTCAAAGAAGATGTTATGCAATTTATGGAAATGAAGGAAATCCCCCCTAGGTGCATAACAGTGTACATGAGGCATCTGTCTGATATGCTGAAGCAATCCAACATGGCCAATATGGTTGGTTTGATGGATCCTTCAAGCATATTAGTTGGGGAAGGCAATTCTGACTACAACTCACTGGTGCTAGCAACCCGGTTGCAGCAAGGACCTGTTGACCAAATACTTTTGGTACCTTACAATTCTGGCTATCATTGGATGCTGACCATCATCAGTGAAGACAAAGAAATATGCTACTTCCTGGACCCTCTTCAACGGTATTTCATGGACCCTCTCCGACGAAGCATGTGTGAGGAAGAATGGAAATATGCTGTAAACAATGGGATTAGACAATTCAACATGGAAACGGGTAGGGGCTTCCGAAAACAACCTCTGTGGAAAGTATTGATGGGTCCTAAACAACCCTCAAATATGGAGTGTGGGTATTATGTGATGAGATACATGAAAGAAATCATAGAAGGTCAAGACCTTTCCTTTGCAACGAAGTGGGATGGGAGGAAGCTAAATGCCTACACACAGACTGAACTGGATGAGGTGAGGTGCGAGTGGACGGACTTTGTCAGTAATTATGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 Transposase_24

Protein Analysis

726

Amino Acids

81.78

Weight (kDa)

5.99

Isoelectric Point (pI)

39.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8039 PF26133 198 - 281 3.2e-13 Domain of unknown function (DUF8039)
Peptidase_C48 PF02902 415 - 543 4.4e-07 Ulp1 protease family, C-terminal catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000321)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221
prunus_persica Prupe.6G157700_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1
pyrus_communis pycom03g09490 pycom03g19580 pycom07g21940 pycom07g27440 pycom14g09710
rosa_chinensis RchiOBHm_Chr1g0318231 RchiOBHm_Chr1g0318241 RchiOBHm_Chr1g0325001 RchiOBHm_Chr1g0325021 RchiOBHm_Chr2g0112301 RchiOBHm_Chr3g0482281 RchiOBHm_Chr3g0482291 RchiOBHm_Chr4g0390561 RchiOBHm_Chr4g0390571 RchiOBHm_Chr4g0417951 RchiOBHm_Chr7g0232611 RchiOBHm_Chr7g0240601
rosa_laevigata RLG00000001069 RLG00000013674 RLG00000018830 RLG00000020747
rosa_multiflora Rmu_co8364947.1_g000001 Rmu_co8416715.1_g000001 Rmu_sc0001348.1_g000014 Rmu_sc0002986.1_g000026 Rmu_sc0004003.1_g000007 Rmu_sc0005137.1_g000014 Rmu_sc0006583.1_g000016
rosa_roxburghii Rroxscaffold_159G00432800 Rroxscaffold_174G00435170 Rroxscaffold_1G00001870 Rroxscaffold_1G00044480 Rroxscaffold_2G00111880 Rroxscaffold_2G00111890 Rroxscaffold_2G00119670 Rroxscaffold_2G00129450 Rroxscaffold_2G00131520 Rroxscaffold_3G00240880 Rroxscaffold_5G00337440 Rroxscaffold_5G00356220 Rroxscaffold_5G00362390 Rroxscaffold_5G00368260 Rroxscaffold_7G00196230 Rroxscaffold_7G00196240 Rroxscaffold_7G00202020
rosa_rugosa Rorug01G0094700 Rorug02G0050500 Rorug02G0179800 Rorug05G0042800 Rorug05G0043000 Rorug05G0043100 Rorug05G0043200 Rorug06G0045300 Rorug06G0045600 Rorug06G0069400 Rorug07G0213000 Rorug07G0213000
rosa_samantha Rh1AG031600 Rh1DG131700 Rh1DG134800 Rh1DG288000 Rh1DG288100 Rh2AG218500 Rh2AG232500 Rh2BG245900 Rh2CG236700 Rh2DG224300 Rh2DG224400 Rh2DG240200 Rh3AG247300 Rh3BG126600 Rh3BG126700 Rh3BG333600 Rh5AG440500 Rh5AG440600 Rh5BG457800 Rh5DG472700 Rh5DG472800 Rh6BG138000 Rh6BG138100 Rh6BG171500 Rh6BG188400 Rh6BG230000 Rh6CG135600 Rh6CG135700 Rh6CG186300 Rh6CG450300 Rh7AG376100 Rh7CG394800 Rh7CG426100 Rh7CG426200 Rh7DG247700 Rh7DG322600 Rh7DG334300 Rh7DG364700
rosa_wichuraiana Rw2G018040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1440
Acc65I GGTACC 1 cut(s) 1774
AccB1I GGYRCC 1 cut(s) 1774
AccB7I CCANNNNNTGG 1 cut(s) 360
AccII CGCG 1 cut(s) 220
AciI CCGC 1 cut(s) 1035
AclI AACGTT 1 cut(s) 1158
AclWI GGATC 6 cut(s) 471, 762, 775, 971, 1670, 1683
AcoI YGGCCR 3 cut(s) 981, 1343, 1653
AcsI RAATTY 2 cut(s) 282, 716
AcuI CTGAAG 3 cut(s) 18, 293, 1658
AfaI GTAC 7 cut(s) 168, 223, 477, 1174, 1390, 1613, 1776
AfeI AGCGCT 1 cut(s) 1275
AfiI CCNNNNNNNGG 3 cut(s) 69, 360, 1295
AflIII ACRYGT 1 cut(s) 1240
AhdI GACNNNNNGTC 1 cut(s) 784
AjnI CCWGG 2 cut(s) 978, 1842
AluBI AGCT 5 cut(s) 249, 1085, 1322, 1450, 2104
AluI AGCT 5 cut(s) 249, 1085, 1322, 1450, 2104
Alw21I GWGCWC 1 cut(s) 124
Alw26I GTCTC 1 cut(s) 686
AlwI GGATC 6 cut(s) 471, 762, 775, 971, 1670, 1683
Ama87I CYCGRG 1 cut(s) 1404
Aor51HI AGCGCT 1 cut(s) 1275
AoxI GGCC 4 cut(s) 251, 981, 1343, 1653
ApeKI GCWGC 6 cut(s) 350, 710, 916, 1193, 1276, 1744
ApoI RAATTY 2 cut(s) 282, 716
ArsI GACNNNNNNTTYG 2 cut(s) 2060, 2092
Asp718I GGTACC 1 cut(s) 1774
AspA2I CCTAGG 1 cut(s) 1595
AspLEI GCGC 3 cut(s) 220, 319, 1276
AspS9I GGNCC 4 cut(s) 1751, 1846, 1870, 1994
AsuC2I CCSGG 2 cut(s) 70, 1738
AsuHPI GGTGA 4 cut(s) 852, 983, 1499, 2149
AsuNHI GCTAGC 1 cut(s) 1729
AvaI CYCGRG 1 cut(s) 1404
AvaII GGWCC 4 cut(s) 1751, 1846, 1870, 1994
AvrII CCTAGG 1 cut(s) 1595
AxyI CCTNAGG 1 cut(s) 255
BaeGI GKGCMC 1 cut(s) 110
BalI TGGCCA 3 cut(s) 983, 1345, 1655
BamHI GGATCC 2 cut(s) 767, 1675
BanI GGYRCC 1 cut(s) 1774
BbsI GAAGAC 2 cut(s) 438, 1827
Bbv12I GWGCWC 1 cut(s) 124
BbvI GCAGC 6 cut(s) 362, 697, 928, 1205, 1263, 1756
BccI CCATC 9 cut(s) 187, 242, 295, 1474, 1478, 1666, 1817, 1984, 2087
BciT130I CCWGG 2 cut(s) 980, 1844
BcnI CCSGG 2 cut(s) 70, 1738
BcoDI GTCTC 1 cut(s) 686
BfaI CTAG 4 cut(s) 321, 1262, 1596, 1730
BfmI CTRYAG 4 cut(s) 348, 696, 711, 1323
BfoI RGCGCY 2 cut(s) 320, 1277
BfuAI ACCTGC 1 cut(s) 1440
BisI GCNGC 6 cut(s) 351, 711, 917, 1194, 1277, 1745
BlnI CCTAGG 1 cut(s) 1595
BlpI GCTNAGC 1 cut(s) 1467
BlsI GCNGC 6 cut(s) 352, 712, 918, 1195, 1278, 1746
BmcAI AGTACT 1 cut(s) 1390
Bme1390I CCNGG 4 cut(s) 70, 980, 1738, 1844
Bme18I GGWCC 4 cut(s) 1751, 1846, 1870, 1994
BmeRI GACNNNNNGTC 1 cut(s) 784
BmeT110I CYCGRG 1 cut(s) 1404
BmgT120I GGNCC 4 cut(s) 1751, 1846, 1870, 1994
BmiI GGNNCC 8 cut(s) 42, 231, 769, 1677, 1776, 1848, 1872, 1995
BmrFI CCNGG 4 cut(s) 70, 980, 1738, 1844
BmsI GCATC 6 cut(s) 194, 206, 353, 1180, 1630, 1791
BmtI GCTAGC 1 cut(s) 1733
BpiI GAAGAC 2 cut(s) 438, 1827
BpmI CTGGAG 2 cut(s) 632, 789
Bpu1102I GCTNAGC 1 cut(s) 1467
BpuEI CTTGAG 1 cut(s) 1430
BpuMI CCSGG 2 cut(s) 70, 1738
BsaAI YACGTR 1 cut(s) 1241
BsaBI GATNNNNATC 1 cut(s) 267
BsaJI CCNNGG 4 cut(s) 225, 1168, 1210, 1595
BsaXI ACNNNNNCTCC 4 cut(s) 651, 681, 2009, 2039
Bsc4I CCNNNNNNNGG 3 cut(s) 69, 360, 1295
Bse1I ACTGG 3 cut(s) 1289, 1728, 2133
Bse21I CCTNAGG 1 cut(s) 255
Bse8I GATNNNNATC 1 cut(s) 267
BseBI CCWGG 2 cut(s) 980, 1844
BseDI CCNNGG 4 cut(s) 225, 1168, 1210, 1595
BseGI GGATG 6 cut(s) 898, 1195, 1489, 1806, 2098, 2137
BseJI GATNNNNATC 1 cut(s) 267
BseLI CCNNNNNNNGG 3 cut(s) 69, 360, 1295
BseMII CTCAG 2 cut(s) 881, 1458
BseNI ACTGG 3 cut(s) 1289, 1728, 2133
BseRI GAGGAG 3 cut(s) 53, 855, 996
BseSI GKGCMC 1 cut(s) 110
BseXI GCAGC 6 cut(s) 362, 697, 928, 1205, 1263, 1756
Bsh1236I CGCG 1 cut(s) 220
BshFI GGCC 4 cut(s) 253, 983, 1345, 1655
BshNI GGYRCC 1 cut(s) 1774
BsiHKAI GWGCWC 1 cut(s) 124
BsiHKCI CYCGRG 1 cut(s) 1404
BsiSI CCGG 2 cut(s) 69, 1738
BslFI GGGAC 2 cut(s) 814, 1426
BslI CCNNNNNNNGG 3 cut(s) 69, 360, 1295
BsmAI GTCTC 1 cut(s) 686
BsmFI GGGAC 2 cut(s) 814, 1426
BsmI GAATGC 3 cut(s) 28, 305, 562
BsnI GGCC 4 cut(s) 253, 983, 1345, 1655
BsoBI CYCGRG 1 cut(s) 1404
Bsp1286I GDGCHC 2 cut(s) 110, 124
Bsp1407I TGTACA 1 cut(s) 1611
Bsp143I GATC 5 cut(s) 412, 463, 767, 963, 1675
Bsp1720I GCTNAGC 1 cut(s) 1467
Bsp19I CCATGG 2 cut(s) 1168, 1210
BspACI CCGC 1 cut(s) 1035
BspANI GGCC 4 cut(s) 253, 983, 1345, 1655
BspCNI CTCAG 2 cut(s) 880, 1459
BspFNI CGCG 1 cut(s) 220
BspHI TCATGA 1 cut(s) 1113
BspLI GGNNCC 8 cut(s) 42, 231, 769, 1677, 1776, 1848, 1872, 1995
BspMAI CTGCAG 2 cut(s) 352, 715
BspMI ACCTGC 1 cut(s) 1440
BspOI GCTAGC 1 cut(s) 1733
BspPI GGATC 6 cut(s) 471, 762, 775, 971, 1670, 1683
BspT107I GGYRCC 1 cut(s) 1774
BsrGI TGTACA 1 cut(s) 1611
BsrI ACTGG 3 cut(s) 1289, 1728, 2133
BssECI CCNNGG 4 cut(s) 225, 1168, 1210, 1595
BssMI GATC 5 cut(s) 412, 463, 767, 963, 1675
BssT1I CCWWGG 3 cut(s) 1168, 1210, 1595
Bst2UI CCWGG 2 cut(s) 980, 1844
Bst6I CTCTTC 4 cut(s) 482, 535, 1506, 1857
BstAUI TGTACA 1 cut(s) 1611
BstBAI YACGTR 1 cut(s) 1241
BstC8I GCNNGC 8 cut(s) 216, 251, 708, 1133, 1137, 1320, 1473, 1731
BstDEI CTNAG 5 cut(s) 125, 255, 313, 867, 1467
BstDSI CCRYGG 3 cut(s) 225, 1168, 1210
BstF5I GGATG 6 cut(s) 898, 1195, 1489, 1806, 2098, 2137
BstFNI CGCG 1 cut(s) 220
BstH2I RGCGCY 2 cut(s) 320, 1277
BstHHI GCGC 3 cut(s) 220, 319, 1276
BstKTI GATC 5 cut(s) 415, 466, 770, 966, 1678
BstMAI GTCTC 1 cut(s) 686
BstMBI GATC 5 cut(s) 412, 463, 767, 963, 1675
BstMWI GCNNNNNNNGC 2 cut(s) 350, 1472
BstNI CCWGG 2 cut(s) 980, 1844
BstNSI RCATGY 1 cut(s) 1891
BstSCI CCNGG 4 cut(s) 68, 978, 1736, 1842
BstSFI CTRYAG 4 cut(s) 348, 696, 711, 1323
BstSLI GKGCMC 1 cut(s) 110
BstUI CGCG 1 cut(s) 220
BstV1I GCAGC 6 cut(s) 362, 697, 928, 1205, 1263, 1756
BstV2I GAAGAC 2 cut(s) 438, 1827
BstX2I RGATCY 2 cut(s) 767, 1675
BstYI RGATCY 2 cut(s) 767, 1675
Bsu36I CCTNAGG 1 cut(s) 255
BsuRI GGCC 4 cut(s) 253, 983, 1345, 1655
BtgI CCRYGG 3 cut(s) 225, 1168, 1210
BtgZI GCGATG 1 cut(s) 789
BtsCI GGATG 6 cut(s) 898, 1195, 1489, 1806, 2098, 2137
BtsIMutI CAGTG 7 cut(s) 117, 244, 973, 1504, 1614, 1721, 1822
BveI ACCTGC 1 cut(s) 1440
Cac8I GCNNGC 8 cut(s) 216, 251, 708, 1133, 1137, 1320, 1473, 1731
CciI TCATGA 1 cut(s) 1113
CfoI GCGC 3 cut(s) 220, 319, 1276
Cfr13I GGNCC 4 cut(s) 1751, 1846, 1870, 1994
Csp6I GTAC 7 cut(s) 167, 222, 476, 1173, 1389, 1612, 1775
CspCI CAANNNNNGTGG 2 cut(s) 1958, 1993
CviQI GTAC 7 cut(s) 167, 222, 476, 1173, 1389, 1612, 1775
DdeI CTNAG 5 cut(s) 125, 255, 313, 867, 1467
DpnI GATC 5 cut(s) 414, 465, 769, 965, 1677
DpnII GATC 5 cut(s) 412, 463, 767, 963, 1675
DriI GACNNNNNGTC 1 cut(s) 784
EaeI YGGCCR 3 cut(s) 981, 1343, 1653
Eam1104I CTCTTC 4 cut(s) 482, 535, 1506, 1857
Eam1105I GACNNNNNGTC 1 cut(s) 784
EarI CTCTTC 4 cut(s) 482, 535, 1506, 1857
Eco130I CCWWGG 3 cut(s) 1168, 1210, 1595
Eco47I GGWCC 4 cut(s) 1751, 1846, 1870, 1994
Eco47III AGCGCT 1 cut(s) 1275
Eco57I CTGAAG 3 cut(s) 18, 293, 1658
Eco81I CCTNAGG 1 cut(s) 255
Eco88I CYCGRG 1 cut(s) 1404
EcoO109I RGGNCCY 2 cut(s) 1751, 1994
EcoRII CCWGG 2 cut(s) 978, 1842
EcoT14I CCWWGG 3 cut(s) 1168, 1210, 1595
EcoT22I ATGCAT 1 cut(s) 621
ErhI CCWWGG 3 cut(s) 1168, 1210, 1595
FalI AAGNNNNNCTT 6 cut(s) 479, 511, 615, 647, 1263, 1295
FaqI GGGAC 2 cut(s) 814, 1426
FauNDI CATATG 1 cut(s) 522
Fnu4HI GCNGC 6 cut(s) 351, 711, 917, 1194, 1277, 1745
FokI GGATG 6 cut(s) 905, 1202, 1496, 1813, 2105, 2144
Fsp4HI GCNGC 6 cut(s) 351, 711, 917, 1194, 1277, 1745
FspBI CTAG 4 cut(s) 321, 1262, 1596, 1730
GlaI GCGC 3 cut(s) 219, 318, 1275
GluI GCNGC 6 cut(s) 351, 711, 917, 1194, 1277, 1745
GsuI CTGGAG 2 cut(s) 632, 789
HaeII RGCGCY 2 cut(s) 320, 1277
HaeIII GGCC 4 cut(s) 253, 983, 1345, 1655
HapII CCGG 2 cut(s) 69, 1738
HhaI GCGC 3 cut(s) 220, 319, 1276
Hin6I GCGC 3 cut(s) 218, 317, 1274
HinP1I GCGC 3 cut(s) 218, 317, 1274
HincII GTYRAC 1 cut(s) 1759
HindII GTYRAC 1 cut(s) 1759
HindIII AAGCTT 1 cut(s) 1083
HinfI GANTC 5 cut(s) 268, 490, 802, 839, 1076
HpaII CCGG 2 cut(s) 69, 1738
HphI GGTGA 4 cut(s) 852, 983, 1499, 2149
Hpy166II GTNNAC 8 cut(s) 8, 115, 1429, 1490, 1612, 1759, 1918, 2151
Hpy188III TCNNGA 9 cut(s) 467, 484, 573, 649, 806, 901, 1114, 1406, 2066
Hpy8I GTNNAC 8 cut(s) 8, 115, 1429, 1490, 1612, 1759, 1918, 2151
Hpy99I CGWCG 1 cut(s) 1884
HpyCH4IV ACGT 5 cut(s) 725, 780, 1158, 1240, 1351
HpyF10VI GCNNNNNNNGC 2 cut(s) 350, 1472
HpyF3I CTNAG 5 cut(s) 125, 255, 313, 867, 1467
HpySE526I ACGT 5 cut(s) 725, 780, 1158, 1240, 1351
HspAI GCGC 3 cut(s) 218, 317, 1274
KpnI GGTACC 1 cut(s) 1778
Kzo9I GATC 5 cut(s) 412, 463, 767, 963, 1675
LmnI GCTCC 5 cut(s) 40, 526, 703, 874, 1455
Lsp1109I GCAGC 6 cut(s) 362, 697, 928, 1205, 1263, 1756
LweI GCATC 6 cut(s) 194, 206, 353, 1180, 1630, 1791
MaeI CTAG 4 cut(s) 321, 1262, 1596, 1730
MaeII ACGT 5 cut(s) 725, 780, 1158, 1240, 1351
MaeIII GTNAC 4 cut(s) 570, 758, 1291, 1306
MalI GATC 5 cut(s) 414, 465, 769, 965, 1677
MboI GATC 5 cut(s) 412, 463, 767, 963, 1675
MfeI CAATTG 1 cut(s) 1248
MflI RGATCY 2 cut(s) 767, 1675
MhlI GDGCHC 2 cut(s) 110, 124
MlsI TGGCCA 3 cut(s) 983, 1345, 1655
MluNI TGGCCA 3 cut(s) 983, 1345, 1655
MlyI GAGTC 3 cut(s) 499, 796, 848
MmeI TCCRAC 5 cut(s) 777, 852, 1123, 1671, 1903
Mox20I TGGCCA 3 cut(s) 983, 1345, 1655
Mph1103I ATGCAT 1 cut(s) 621
MscI TGGCCA 3 cut(s) 983, 1345, 1655
MseI TTAA 6 cut(s) 153, 459, 497, 605, 1065, 1359
MslI CAYNNNNRTG 4 cut(s) 209, 624, 989, 1607
Msp20I TGGCCA 3 cut(s) 983, 1345, 1655
MspI CCGG 2 cut(s) 69, 1738
MspR9I CCNGG 4 cut(s) 70, 980, 1738, 1844
MunI CAATTG 1 cut(s) 1248
Mva1269I GAATGC 3 cut(s) 28, 305, 562
MvaI CCWGG 2 cut(s) 980, 1844
MvnI CGCG 1 cut(s) 220
MwoI GCNNNNNNNGC 2 cut(s) 350, 1472
NciI CCSGG 2 cut(s) 70, 1738
NcoI CCATGG 2 cut(s) 1168, 1210
NdeI CATATG 1 cut(s) 522
NdeII GATC 5 cut(s) 412, 463, 767, 963, 1675
NheI GCTAGC 1 cut(s) 1729
NlaIV GGNNCC 8 cut(s) 42, 231, 769, 1677, 1776, 1848, 1872, 1995
NmuCI GTSAC 1 cut(s) 570
NsiI ATGCAT 1 cut(s) 621
NspI RCATGY 1 cut(s) 1891
PaeR7I CTCGAG 1 cut(s) 1404
PagI TCATGA 1 cut(s) 1113
PctI GAATGC 3 cut(s) 28, 305, 562
PfeI GAWTC 2 cut(s) 268, 1076
PflFI GACNNNGTC 1 cut(s) 2159
PflMI CCANNNNNTGG 1 cut(s) 360
PfoI TCCNGGA 1 cut(s) 1842
PkrI GCNGC 6 cut(s) 352, 712, 918, 1195, 1278, 1746
PleI GAGTC 3 cut(s) 498, 796, 847
PpsI GAGTC 3 cut(s) 498, 796, 847
Ppu21I YACGTR 1 cut(s) 1241
PpuMI RGGWCCY 2 cut(s) 1751, 1994
Psp1406I AACGTT 1 cut(s) 1158
Psp5II RGGWCCY 2 cut(s) 1751, 1994
Psp6I CCWGG 2 cut(s) 978, 1842
PspGI CCWGG 2 cut(s) 978, 1842
PspN4I GGNNCC 8 cut(s) 42, 231, 769, 1677, 1776, 1848, 1872, 1995
PspPI GGNCC 4 cut(s) 1751, 1846, 1870, 1994
PspPPI RGGWCCY 2 cut(s) 1751, 1994
PstI CTGCAG 2 cut(s) 352, 715
PsuI RGATCY 2 cut(s) 767, 1675
PsyI GACNNNGTC 1 cut(s) 2159
RsaI GTAC 7 cut(s) 168, 223, 477, 1174, 1390, 1613, 1776
RsaNI GTAC 7 cut(s) 167, 222, 476, 1173, 1389, 1612, 1775
RseI CAYNNNNRTG 4 cut(s) 209, 624, 989, 1607
SaqAI TTAA 6 cut(s) 153, 459, 497, 605, 1065, 1359
SatI GCNGC 6 cut(s) 351, 711, 917, 1194, 1277, 1745
Sau3AI GATC 5 cut(s) 412, 463, 767, 963, 1675
Sau96I GGNCC 4 cut(s) 1751, 1846, 1870, 1994
ScaI AGTACT 1 cut(s) 1390
SchI GAGTC 3 cut(s) 499, 796, 848
ScrFI CCNGG 4 cut(s) 70, 980, 1738, 1844
SduI GDGCHC 2 cut(s) 110, 124
SfaNI GCATC 6 cut(s) 194, 206, 353, 1180, 1630, 1791
SfcI CTRYAG 4 cut(s) 348, 696, 711, 1323
Sfr274I CTCGAG 1 cut(s) 1404
SinI GGWCC 4 cut(s) 1751, 1846, 1870, 1994
SlaI CTCGAG 1 cut(s) 1404
SmiMI CAYNNNNRTG 4 cut(s) 209, 624, 989, 1607
SmlI CTYRAG 2 cut(s) 1404, 1445
SmoI CTYRAG 2 cut(s) 1404, 1445
SsiI CCGC 1 cut(s) 1035
SspI AATATT 2 cut(s) 856, 1546
SspMI CTAG 4 cut(s) 321, 1262, 1596, 1730
StyD4I CCNGG 4 cut(s) 68, 978, 1736, 1842
StyI CCWWGG 3 cut(s) 1168, 1210, 1595
TaiI ACGT 5 cut(s) 728, 783, 1161, 1243, 1354
TaqI TCGA 1 cut(s) 1405
TatI WGTACW 2 cut(s) 1388, 1611
TfiI GAWTC 2 cut(s) 268, 1076
Tru1I TTAA 6 cut(s) 153, 459, 497, 605, 1065, 1359
Tru9I TTAA 6 cut(s) 153, 459, 497, 605, 1065, 1359
TscAI CASTG 7 cut(s) 117, 244, 973, 1504, 1614, 1728, 1822
TseFI GTSAC 1 cut(s) 570
TseI GCWGC 6 cut(s) 350, 710, 916, 1193, 1276, 1744
Tsp45I GTSAC 1 cut(s) 570
TspGWI ACGGA 2 cut(s) 283, 2168
TspRI CASTG 7 cut(s) 117, 244, 973, 1504, 1614, 1728, 1822
Tth111I GACNNNGTC 1 cut(s) 2159
Van91I CCANNNNNTGG 1 cut(s) 360
VpaK11BI GGWCC 4 cut(s) 1751, 1846, 1870, 1994
XapI RAATTY 2 cut(s) 282, 716
XceI RCATGY 1 cut(s) 1891
XcmI CCANNNNNNNNNTGG 1 cut(s) 1208
XhoI CTCGAG 1 cut(s) 1404
XmaJI CCTAGG 1 cut(s) 1595
XspI CTAG 4 cut(s) 321, 1262, 1596, 1730
ZrmI AGTACT 1 cut(s) 1390
Zsp2I ATGCAT 1 cut(s) 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.