Rh7AG376100

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0000321
Physical Location & Seq
Forward (+)
48783998 .. 48832107
48110 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG376100.1

Sequence Viewer

Length: 2973 bp
ATGGCTGAAGTGATGAAATTGATTAGGCTTTTGTTGGTGTTTCTCTCACTACTTTCTCTCTCTACTGCTCAATCTCCAAAAATAATTAGCAACATAACAACATGCCCCATTGATCTCAGCTATGTCCTTAGAGCCCCCGTCAACTTCTCATCATGCAAAAGCTTCCAACCCCCGGCTAAAACCCCCCAAATGGACATCACCCTGATCCCATGTTGTCAAACCTTATGGTACCTCTTTGGAATAGGCCTTGCTCAACACCTCAAGGCCACCACTTTTTTCCTACTCCCCAACATAGCAACCTCAAATTCTTGCCTCCAAAACTACCAGTCCAAGCTTACCTCTCTAGCAATCCCTTCCAATCTTCTCTCTTATTGTTTTGACCCTTTACTGTTTGTAACTGGTCCCAATTTCTGTGCCCAAATTGAATCTTCCCAAGATTTGATCTCCAAGCTTAATCAGACCACTACAATTGACTCTGGATGCAAGCCAGACCTCGCAGATCTCTCACCCTGTGATGTTTGCGCAACAACTATGTTGCAAATTCATCAACAATTAATCACAGTTGATGATCCAAATCATGTCATTTCTACCTACTGGGGAGATAAAGAGAACGTAGTGTACTGGCTAGCAAAATCATCATGCAAAACAGTAAGCGGCACATTGATCATTCATAATGTTATTAAACAGAGAGCAGTAGATGCTACATGCTTGCTGCGTGAGCATGAAGAGTTGTTGAAGATTCAGCATCCTAAAAAGTCAAAAAGTGAGAAGTGGCTTCGAGATGAACAGAACAGAACTTTCCAAGATTGGTTGAAACAAAAAGTTGAGAATGAGCTTGCTGATCCCAATAATGACATATCTAATACTTTGAAGTGGGTAGCAGAAGGGCCTCGAGAGACAGTGAAAAAGTATGCTGGATATTCTATAGATGGCTGCCATTTTCACACGAAGTCTCATGATAGTTCTCATAATCTTGCAGTTCTAGCCAAGATGAAACACTTCAAAGACAATCATTTAGAATGCGATGATGAATATACGGATGAGGATGCAGATGACCAGGCCACAAACAAGAGGAAGGGGAGAGGTCCAACTTTGATGTCAGACTTGGTCCATCGTAGGGGCGGTAAGGGTCGTATAGAAGTTGGATATAACACAAAAGGACAGCCAGAAGGCAAGCATGGATCAAATTTAGCTAGTTTCATAGGAGTAATGGCGCGAAGCACTGTTCCAATTATATATGAAGATTGGAAAGCGGTACCACCTTCTTTAAAGGATAAGATATGGGACATGGTTCTGAAAGCATTCATCGTGGATATGAATAGTAGGAAGCATGTCATAAGCAGTGCTGGAAAGGCATGGAAATCATTCAAGAGCACTTTAACATCATCATATATATTGAAATTCAAACATCAGCCTCACAAGCTAAAGACTCCACCTCTTGGCTATGATTTCATCCTTCAAACACATTGGGATGCCTTTGTGAAGTCTCGATTAACAGAAGAATTTGCAGAAATTAGAGAAGCTGCATCAAGGAGGCGAGCTCTCTATGTATACAATCATCGAATGTCTAGAAAAGGCTATGCCAAATTGGAAGAAGAACTAAAGTTGGCTTCAGGTGACCAAAATGATATTGATCGGTCAGTTCTGTGGAAGAAAGCACGTGTTGATAAAGATGGAAACTACTCAAATGAGGCAGTGAAACAACGTGTTGATAAAATTGATGAAGTGACTAAACATGTTGAGGATGGTATTATATCAACAGCCGGTAGTAATGACATACTAGCAATGGCTTTGGAAAAGCCTGAATATTCCGGTCGTGTAAGAGGTGTTGGAAGCAATGTCACACCTATTTTGTACTTCAATACTCCAAGGTGTCGATCTCAATCCACCCAAAATCAGTTGCTGCAACAAATGGAGCAAATGCAGAACCAGCTTTTAATGTTTACTCAACTGCTACAACCTGATCAATTGTTAATGATGCAACACATGGTCCAAGGTAGCCGTGTGTCTGACAAATCTAGTTGCTCAGTTCAGAAAGACAAATATGAGACATTATCTAAGGAGGAGGTGTCTAGGGTGGAAATGATGAAAGGAATTGAGAAGAAATCTAAGAAAGCTACATTTAACGTCAATGTACAAGAGGCTTTGAAGAGTAGTACTAAAATTCCAGAAGAAATCAAGAAGGTAAAGCAACACAATAAAGAAGAATCAGCTTTGAAACCTGGAAAAAACAAACAAGATGGCTATACAGAGGTTCCCAAAGACAAGCCACAAGATATTGAGAAGCCCAAGCTAAAGATTCCAAATGCATCATTTAAACACTCGTTGCCACAAAACTTGTACAAGATGCCTCTATCTGTGCGATTTCTATTGCGTTTTGCTAAGACAATGCGAGAAGAAAAACCATTGAAGGTGGCCATTGAGTCTGGTGTCTTCGGATATGAACAAGACACTTATCTGAACAAAGATGACGTCATTGAATTTTGTTCGATGGAACCAGTCTCTTCTGTTTGCATGACAATCTACATGAGACATTTGTGGTCATTATTAAAGAAAGATCATAAGGATAACTTGTATGGATTTGCGGATCCATCTTCCTTCTCTCATGATTCTGGAAAACGAGATGAAAGATCGTTTGCATTGTCAATAAGATTAGAGAAAAGCAAGGAACAGCAATTGATTTTTGCTCCTTACAATTTTGGGTTTCACTGGGTTATGGTTGTAATTAATCCTTATAGTGATATGGTGTATTACTTGGACTCTCTTAATCTTGTTGACATAGACCATGGATTAAAGGGTGTTATAGATGTCGCTCTAAGGATGTTTAATGCCCAAAAAGGAAGGAATCGAAAAAATGCTATTTGGAAAATCATACAGTTCAACAACCAAAGTTACAATCAAGAAGAGATAGATGATGTACGAGTAGAGTGGGCTGATCTTGTTGAAACATTACTTCAAGATGATGTAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 Transposase_24

Protein Analysis

990

Amino Acids

113.05

Weight (kDa)

8.66

Isoelectric Point (pI)

40.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPARK PF19160 33 - 193 3.4e-31 SPARK
Transposase_24 PF03004 487 - 616 4.5e-14 Plant transposase (Ptta/En/Spm family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000321)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221
prunus_persica Prupe.6G157700_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1
pyrus_communis pycom03g09490 pycom03g19580 pycom07g21940 pycom07g27440 pycom14g09710
rosa_chinensis RchiOBHm_Chr1g0318231 RchiOBHm_Chr1g0318241 RchiOBHm_Chr1g0325001 RchiOBHm_Chr1g0325021 RchiOBHm_Chr2g0112301 RchiOBHm_Chr3g0482281 RchiOBHm_Chr3g0482291 RchiOBHm_Chr4g0390561 RchiOBHm_Chr4g0390571 RchiOBHm_Chr4g0417951 RchiOBHm_Chr7g0232611 RchiOBHm_Chr7g0240601
rosa_laevigata RLG00000001069 RLG00000013674 RLG00000018830 RLG00000020747
rosa_multiflora Rmu_co8364947.1_g000001 Rmu_co8416715.1_g000001 Rmu_sc0001348.1_g000014 Rmu_sc0002986.1_g000026 Rmu_sc0004003.1_g000007 Rmu_sc0005137.1_g000014 Rmu_sc0006583.1_g000016
rosa_roxburghii Rroxscaffold_159G00432800 Rroxscaffold_174G00435170 Rroxscaffold_1G00001870 Rroxscaffold_1G00044480 Rroxscaffold_2G00111880 Rroxscaffold_2G00111890 Rroxscaffold_2G00119670 Rroxscaffold_2G00129450 Rroxscaffold_2G00131520 Rroxscaffold_3G00240880 Rroxscaffold_5G00337440 Rroxscaffold_5G00356220 Rroxscaffold_5G00362390 Rroxscaffold_5G00368260 Rroxscaffold_7G00196230 Rroxscaffold_7G00196240 Rroxscaffold_7G00202020
rosa_rugosa Rorug01G0094700 Rorug02G0050500 Rorug02G0179800 Rorug05G0042800 Rorug05G0043000 Rorug05G0043100 Rorug05G0043200 Rorug06G0045300 Rorug06G0045600 Rorug06G0069400 Rorug07G0213000 Rorug07G0213000
rosa_samantha Rh1AG031600 Rh1DG131700 Rh1DG134800 Rh1DG288000 Rh1DG288100 Rh2AG218500 Rh2AG232500 Rh2BG245900 Rh2CG236700 Rh2DG224300 Rh2DG224400 Rh2DG240200 Rh3AG247300 Rh3BG126600 Rh3BG126700 Rh3BG333600 Rh5AG440500 Rh5AG440600 Rh5BG457800 Rh5DG472700 Rh5DG472800 Rh6BG138000 Rh6BG138100 Rh6BG171500 Rh6BG188400 Rh6BG230000 Rh6CG135600 Rh6CG135700 Rh6CG186300 Rh6CG450300 Rh7AG376100 Rh7CG394800 Rh7CG426100 Rh7CG426200 Rh7DG247700 Rh7DG322600 Rh7DG334300 Rh7DG364700
rosa_wichuraiana Rw2G018040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2475
Acc16I TGCGCA 1 cut(s) 523
Acc65I GGTACC 2 cut(s) 228, 1255
AccB1I GGYRCC 2 cut(s) 228, 1255
AccB7I CCANNNNNTGG 1 cut(s) 1439
AccI GTMKAC 1 cut(s) 1551
AccII CGCG 1 cut(s) 1216
AciI CCGC 4 cut(s) 654, 1122, 1253, 2585
AclWI GGATC 6 cut(s) 199, 563, 836, 1189, 2582, 2595
AcoI YGGCCR 1 cut(s) 2415
AcsI RAATTY 7 cut(s) 304, 540, 1186, 1400, 1502, 2163, 2480
AcuI CTGAAG 2 cut(s) 27, 1596
AcvI CACGTG 1 cut(s) 1661
AcyI GRCGYC 1 cut(s) 2472
AdeI CACNNNGTG 1 cut(s) 512
AfaI GTAC 8 cut(s) 230, 620, 1257, 1856, 2136, 2158, 2342, 2919
AfiI CCNNNNNNNGG 4 cut(s) 172, 190, 1117, 1439
AflIII ACRYGT 3 cut(s) 1660, 1705, 1735
AjnI CCWGG 2 cut(s) 1056, 2221
Alw21I GWGCWC 2 cut(s) 1376, 1543
Alw26I GTCTC 6 cut(s) 890, 957, 1491, 2042, 2506, 2524
AlwI GGATC 6 cut(s) 199, 563, 836, 1189, 2582, 2595
AlwNI CAGNNNCTG 1 cut(s) 1903
Ama87I CYCGRG 1 cut(s) 891
AoxI GGCC 5 cut(s) 244, 264, 887, 1059, 2415
ApeKI GCWGC 4 cut(s) 712, 933, 1523, 1903
ApoI RAATTY 7 cut(s) 304, 540, 1186, 1400, 1502, 2163, 2480
ArsI GACNNNNNNTTYG 2 cut(s) 2457, 2489
AseI ATTAAT 2 cut(s) 554, 2727
Asp700I GAANNNNTTC 3 cut(s) 998, 1301, 1364
Asp718I GGTACC 2 cut(s) 228, 1255
AspLEI GCGC 2 cut(s) 524, 1216
AspS9I GGNCC 5 cut(s) 401, 887, 1085, 1108, 1990
AsuC2I CCSGG 1 cut(s) 173
AsuHPI GGTGA 3 cut(s) 190, 498, 1628
AsuNHI GCTAGC 1 cut(s) 625
AvaI CYCGRG 1 cut(s) 891
AvaII GGWCC 4 cut(s) 401, 1085, 1108, 1990
BaeGI GKGCMC 1 cut(s) 418
BalI TGGCCA 1 cut(s) 2417
BamHI GGATCC 1 cut(s) 2587
BanI GGYRCC 2 cut(s) 228, 1255
BanII GRGCYC 2 cut(s) 136, 1543
BbrPI CACGTG 1 cut(s) 1661
BbsI GAAGAC 1 cut(s) 2425
Bbv12I GWGCWC 2 cut(s) 1376, 1543
BbvI GCAGC 4 cut(s) 699, 920, 1510, 1890
BccI CCATC 7 cut(s) 923, 1119, 1667, 1739, 2234, 2485, 2599
BceAI ACGGC 1 cut(s) 1986
BciT130I CCWGG 2 cut(s) 1058, 2223
BclI TGATCA 2 cut(s) 663, 1963
BcnI CCSGG 1 cut(s) 173
BcoDI GTCTC 6 cut(s) 890, 957, 1491, 2042, 2506, 2524
BfaI CTAG 8 cut(s) 344, 626, 983, 1194, 1569, 1781, 2019, 2073
BfmI CTRYAG 1 cut(s) 924
BglII AGATCT 1 cut(s) 499
BisI GCNGC 5 cut(s) 655, 713, 934, 1524, 1904
BlsI GCNGC 5 cut(s) 656, 714, 935, 1525, 1905
BmcAI AGTACT 1 cut(s) 2158
Bme1390I CCNGG 3 cut(s) 173, 1058, 2223
Bme18I GGWCC 4 cut(s) 401, 1085, 1108, 1990
BmeT110I CYCGRG 1 cut(s) 891
BmgT120I GGNCC 5 cut(s) 401, 887, 1085, 1108, 1990
BmiI GGNNCC 6 cut(s) 230, 403, 1257, 2256, 2496, 2589
BmrFI CCNGG 3 cut(s) 173, 1058, 2223
BmrI ACTGGG 2 cut(s) 604, 2719
BmsI GCATC 9 cut(s) 470, 688, 754, 1036, 1462, 1535, 1968, 2318, 2337
BmtI GCTAGC 1 cut(s) 629
BmuI ACTGGG 2 cut(s) 604, 2719
BpiI GAAGAC 1 cut(s) 2425
BplI GAGNNNNNCTC 2 cut(s) 1525, 1557
BpuEI CTTGAG 1 cut(s) 245
BpuMI CCSGG 1 cut(s) 173
BsaAI YACGTR 1 cut(s) 1661
BsaBI GATNNNNATC 3 cut(s) 573, 1632, 1882
BsaHI GRCGYC 1 cut(s) 2472
BsaJI CCNNGG 4 cut(s) 171, 1868, 1993, 2785
BsaWI WCCGGW 1 cut(s) 1811
BsaXI ACNNNNNCTCC 2 cut(s) 2054, 2084
Bsc4I CCNNNNNNNGG 4 cut(s) 172, 190, 1117, 1439
Bse118I RCCGGY 1 cut(s) 1763
Bse1I ACTGG 6 cut(s) 325, 403, 599, 626, 2498, 2714
Bse3DI GCAATG 2 cut(s) 1791, 1843
Bse8I GATNNNNATC 3 cut(s) 573, 1632, 1882
BseBI CCWGG 2 cut(s) 1058, 2223
BseDI CCNNGG 4 cut(s) 171, 1868, 1993, 2785
BseGI GGATG 8 cut(s) 485, 745, 1045, 1051, 1452, 1477, 1750, 2826
BseJI GATNNNNATC 3 cut(s) 573, 1632, 1882
BseLI CCNNNNNNNGG 4 cut(s) 172, 190, 1117, 1439
BseMI GCAATG 2 cut(s) 1791, 1843
BseMII CTCAG 2 cut(s) 130, 2040
BseNI ACTGG 6 cut(s) 325, 403, 599, 626, 2498, 2714
BseRI GAGGAG 1 cut(s) 2078
BseSI GKGCMC 1 cut(s) 418
BseXI GCAGC 4 cut(s) 699, 920, 1510, 1890
Bsh1236I CGCG 1 cut(s) 1216
Bsh1285I CGRYCG 1 cut(s) 1816
BshFI GGCC 5 cut(s) 246, 266, 889, 1061, 2417
BshNI GGYRCC 2 cut(s) 228, 1255
BsiEI CGRYCG 1 cut(s) 1816
BsiHKAI GWGCWC 2 cut(s) 1376, 1543
BsiHKCI CYCGRG 1 cut(s) 891
BsiSI CCGG 3 cut(s) 173, 1764, 1812
BslFI GGGAC 2 cut(s) 387, 1298
BslI CCNNNNNNNGG 4 cut(s) 172, 190, 1117, 1439
BsmAI GTCTC 6 cut(s) 890, 957, 1491, 2042, 2506, 2524
BsmFI GGGAC 2 cut(s) 387, 1298
BsmI GAATGC 2 cut(s) 1025, 1301
BsnI GGCC 5 cut(s) 246, 266, 889, 1061, 2417
BsoBI CYCGRG 1 cut(s) 891
Bsp1286I GDGCHC 4 cut(s) 136, 418, 1376, 1543
Bsp1407I TGTACA 2 cut(s) 2134, 2340
Bsp19I CCATGG 1 cut(s) 2785
BspACI CCGC 4 cut(s) 654, 1122, 1253, 2585
BspANI GGCC 5 cut(s) 246, 266, 889, 1061, 2417
BspCNI CTCAG 2 cut(s) 129, 2039
BspFNI CGCG 1 cut(s) 1216
BspHI TCATGA 2 cut(s) 955, 2605
BspLI GGNNCC 6 cut(s) 230, 403, 1257, 2256, 2496, 2589
BspOI GCTAGC 1 cut(s) 629
BspPI GGATC 6 cut(s) 199, 563, 836, 1189, 2582, 2595
BspT107I GGYRCC 2 cut(s) 228, 1255
BsrDI GCAATG 2 cut(s) 1791, 1843
BsrFI RCCGGY 1 cut(s) 1763
BsrGI TGTACA 2 cut(s) 2134, 2340
BsrI ACTGG 6 cut(s) 325, 403, 599, 626, 2498, 2714
BssAI RCCGGY 1 cut(s) 1763
BssECI CCNNGG 4 cut(s) 171, 1868, 1993, 2785
BssNAI GTATAC 1 cut(s) 1552
BssNI GRCGYC 1 cut(s) 2472
BssT1I CCWWGG 3 cut(s) 1868, 1993, 2785
Bst1107I GTATAC 1 cut(s) 1552
Bst2UI CCWGG 2 cut(s) 1058, 2223
Bst4CI ACNGT 6 cut(s) 390, 562, 649, 901, 1225, 2877
Bst6I CTCTTC 4 cut(s) 720, 2144, 2509, 2898
BstACI GRCGYC 1 cut(s) 2472
BstAPI GCANNNNNTGC 1 cut(s) 698
BstAUI TGTACA 2 cut(s) 2134, 2340
BstBAI YACGTR 1 cut(s) 1661
BstC8I GCNNGC 6 cut(s) 485, 627, 710, 837, 1175, 1539
BstDEI CTNAG 7 cut(s) 116, 128, 2026, 2058, 2109, 2382, 2816
BstDSI CCRYGG 1 cut(s) 2785
BstEII GGTNACC 1 cut(s) 1616
BstF5I GGATG 8 cut(s) 485, 745, 1045, 1051, 1452, 1477, 1750, 2826
BstFNI CGCG 1 cut(s) 1216
BstHHI GCGC 2 cut(s) 524, 1216
BstMAI GTCTC 6 cut(s) 890, 957, 1491, 2042, 2506, 2524
BstMCI CGRYCG 1 cut(s) 1816
BstMWI GCNNNNNNNGC 6 cut(s) 698, 718, 983, 1352, 1420, 1930
BstNI CCWGG 2 cut(s) 1058, 2223
BstNSI RCATGY 4 cut(s) 105, 708, 1334, 1739
BstPI GGTNACC 1 cut(s) 1616
BstSCI CCNGG 3 cut(s) 171, 1056, 2221
BstSFI CTRYAG 1 cut(s) 924
BstSLI GKGCMC 1 cut(s) 418
BstUI CGCG 1 cut(s) 1216
BstV1I GCAGC 4 cut(s) 699, 920, 1510, 1890
BstV2I GAAGAC 1 cut(s) 2425
BstX2I RGATCY 2 cut(s) 499, 2587
BstYI RGATCY 2 cut(s) 499, 2587
BstZ17I GTATAC 1 cut(s) 1552
BsuRI GGCC 5 cut(s) 246, 266, 889, 1061, 2417
BtgI CCRYGG 1 cut(s) 2785
BtgZI GCGATG 1 cut(s) 1038
BtsCI GGATG 8 cut(s) 485, 745, 1045, 1051, 1452, 1477, 1750, 2826
BtsI GCAGTG 2 cut(s) 1348, 1701
BtsIMutI CAGTG 5 cut(s) 906, 1221, 1348, 1701, 2707
Cac8I GCNNGC 6 cut(s) 485, 627, 710, 837, 1175, 1539
CaiI CAGNNNCTG 1 cut(s) 1903
CciI TCATGA 2 cut(s) 955, 2605
CfoI GCGC 2 cut(s) 524, 1216
Cfr10I RCCGGY 1 cut(s) 1763
Cfr13I GGNCC 5 cut(s) 401, 887, 1085, 1108, 1990
Csp6I GTAC 8 cut(s) 229, 619, 1256, 1855, 2135, 2157, 2341, 2918
CspCI CAANNNNNGTGG 4 cut(s) 451, 486, 2319, 2354
CviQI GTAC 8 cut(s) 229, 619, 1256, 1855, 2135, 2157, 2341, 2918
DdeI CTNAG 7 cut(s) 116, 128, 2026, 2058, 2109, 2382, 2816
DraI TTTAAA 2 cut(s) 1269, 2317
DraIII CACNNNGTG 1 cut(s) 512
EaeI YGGCCR 1 cut(s) 2415
Eam1104I CTCTTC 4 cut(s) 720, 2144, 2509, 2898
EarI CTCTTC 4 cut(s) 720, 2144, 2509, 2898
Ecl136II GAGCTC 1 cut(s) 1541
Eco130I CCWWGG 3 cut(s) 1868, 1993, 2785
Eco147I AGGCCT 1 cut(s) 246
Eco24I GRGCYC 2 cut(s) 136, 1543
Eco47I GGWCC 4 cut(s) 401, 1085, 1108, 1990
Eco53kI GAGCTC 1 cut(s) 1541
Eco57I CTGAAG 2 cut(s) 27, 1596
Eco72I CACGTG 1 cut(s) 1661
Eco88I CYCGRG 1 cut(s) 891
Eco91I GGTNACC 1 cut(s) 1616
EcoICRI GAGCTC 1 cut(s) 1541
EcoO109I RGGNCCY 1 cut(s) 887
EcoO65I GGTNACC 1 cut(s) 1616
EcoRII CCWGG 2 cut(s) 1056, 2221
EcoT14I CCWWGG 3 cut(s) 1868, 1993, 2785
EcoT22I ATGCAT 1 cut(s) 2311
EcoT38I GRGCYC 2 cut(s) 136, 1543
ErhI CCWWGG 3 cut(s) 1868, 1993, 2785
FalI AAGNNNNNCTT 2 cut(s) 2555, 2587
FaqI GGGAC 2 cut(s) 387, 1298
FbaI TGATCA 2 cut(s) 663, 1963
FblI GTMKAC 1 cut(s) 1551
Fnu4HI GCNGC 5 cut(s) 655, 713, 934, 1524, 1904
FokI GGATG 8 cut(s) 492, 732, 1052, 1058, 1439, 1484, 1757, 2833
FriOI GRGCYC 2 cut(s) 136, 1543
Fsp4HI GCNGC 5 cut(s) 655, 713, 934, 1524, 1904
FspBI CTAG 8 cut(s) 344, 626, 983, 1194, 1569, 1781, 2019, 2073
FspI TGCGCA 1 cut(s) 523
GlaI GCGC 2 cut(s) 523, 1215
GluI GCNGC 5 cut(s) 655, 713, 934, 1524, 1904
HaeIII GGCC 5 cut(s) 246, 266, 889, 1061, 2417
HapII CCGG 3 cut(s) 173, 1764, 1812
HhaI GCGC 2 cut(s) 524, 1216
Hin1I GRCGYC 1 cut(s) 2472
Hin6I GCGC 2 cut(s) 522, 1214
HinP1I GCGC 2 cut(s) 522, 1214
HincII GTYRAC 2 cut(s) 142, 2776
HindII GTYRAC 2 cut(s) 142, 2776
HindIII AAGCTT 3 cut(s) 160, 332, 449
HpaII CCGG 3 cut(s) 173, 1764, 1812
HphI GGTGA 3 cut(s) 190, 498, 1628
Hpy166II GTNNAC 5 cut(s) 142, 619, 1552, 1944, 2776
Hpy188I TCNGA 7 cut(s) 459, 1102, 1296, 2011, 2034, 2438, 2460
Hpy8I GTNNAC 5 cut(s) 142, 619, 1552, 1944, 2776
HpyCH4III ACNGT 6 cut(s) 390, 562, 649, 901, 1225, 2877
HpyCH4IV ACGT 5 cut(s) 612, 1660, 1705, 2127, 2472
HpyF10VI GCNNNNNNNGC 6 cut(s) 698, 718, 983, 1352, 1420, 1930
HpyF3I CTNAG 7 cut(s) 116, 128, 2026, 2058, 2109, 2382, 2816
HpySE526I ACGT 5 cut(s) 612, 1660, 1705, 2127, 2472
Hsp92I GRCGYC 1 cut(s) 2472
HspAI GCGC 2 cut(s) 522, 1214
KpnI GGTACC 2 cut(s) 232, 1259
Ksp22I TGATCA 2 cut(s) 663, 1963
LmnI GCTCC 2 cut(s) 1915, 2692
Lsp1109I GCAGC 4 cut(s) 699, 920, 1510, 1890
LweI GCATC 9 cut(s) 470, 688, 754, 1036, 1462, 1535, 1968, 2318, 2337
MaeI CTAG 8 cut(s) 344, 626, 983, 1194, 1569, 1781, 2019, 2073
MaeII ACGT 5 cut(s) 612, 1660, 1705, 2127, 2472
MaeIII GTNAC 5 cut(s) 394, 1616, 1726, 1840, 2891
MfeI CAATTG 3 cut(s) 468, 1967, 2675
MflI RGATCY 2 cut(s) 499, 2587
MhlI GDGCHC 4 cut(s) 136, 418, 1376, 1543
MlsI TGGCCA 1 cut(s) 2417
MluNI TGGCCA 1 cut(s) 2417
MlyI GAGTC 4 cut(s) 467, 1423, 2432, 2753
MmeI TCCRAC 4 cut(s) 190, 1112, 1123, 1810
Mox20I TGGCCA 1 cut(s) 2417
Mph1103I ATGCAT 1 cut(s) 2311
MroXI GAANNNNTTC 3 cut(s) 998, 1301, 1364
MscI TGGCCA 1 cut(s) 2417
MslI CAYNNNNRTG 1 cut(s) 1470
Msp20I TGGCCA 1 cut(s) 2417
MspI CCGG 3 cut(s) 173, 1764, 1812
MspR9I CCNGG 3 cut(s) 173, 1058, 2223
MunI CAATTG 3 cut(s) 468, 1967, 2675
Mva1269I GAATGC 2 cut(s) 1025, 1301
MvaI CCWGG 2 cut(s) 1058, 2223
MvnI CGCG 1 cut(s) 1216
MwoI GCNNNNNNNGC 6 cut(s) 698, 718, 983, 1352, 1420, 1930
NciI CCSGG 1 cut(s) 173
NcoI CCATGG 1 cut(s) 2785
NheI GCTAGC 1 cut(s) 625
NlaIV GGNNCC 6 cut(s) 230, 403, 1257, 2256, 2496, 2589
NmuCI GTSAC 3 cut(s) 1616, 1726, 1840
NsbI TGCGCA 1 cut(s) 523
NsiI ATGCAT 1 cut(s) 2311
NspI RCATGY 4 cut(s) 105, 708, 1334, 1739
PaeR7I CTCGAG 1 cut(s) 891
PagI TCATGA 2 cut(s) 955, 2605
PceI AGGCCT 1 cut(s) 246
PciI ACATGT 1 cut(s) 1735
PctI GAATGC 2 cut(s) 1025, 1301
PdmI GAANNNNTTC 3 cut(s) 998, 1301, 1364
PfeI GAWTC 6 cut(s) 425, 739, 2207, 2299, 2609, 2845
PflFI GACNNNGTC 1 cut(s) 1106
PflMI CCANNNNNTGG 1 cut(s) 1439
PkrI GCNGC 5 cut(s) 656, 714, 935, 1525, 1905
PleI GAGTC 4 cut(s) 467, 1423, 2431, 2753
PmaCI CACGTG 1 cut(s) 1661
PmlI CACGTG 1 cut(s) 1661
PpsI GAGTC 4 cut(s) 467, 1423, 2431, 2753
Ppu21I YACGTR 1 cut(s) 1661
PscI ACATGT 1 cut(s) 1735
PshBI ATTAAT 2 cut(s) 554, 2727
Psp124BI GAGCTC 1 cut(s) 1543
Psp6I CCWGG 2 cut(s) 1056, 2221
PspCI CACGTG 1 cut(s) 1661
PspEI GGTNACC 1 cut(s) 1616
PspGI CCWGG 2 cut(s) 1056, 2221
PspN4I GGNNCC 6 cut(s) 230, 403, 1257, 2256, 2496, 2589
PspPI GGNCC 5 cut(s) 401, 887, 1085, 1108, 1990
PsrI GAACNNNNNNTAC 2 cut(s) 602, 634
PstNI CAGNNNCTG 1 cut(s) 1903
PsuI RGATCY 2 cut(s) 499, 2587
PsyI GACNNNGTC 1 cut(s) 1106
RsaI GTAC 8 cut(s) 230, 620, 1257, 1856, 2136, 2158, 2342, 2919
RsaNI GTAC 8 cut(s) 229, 619, 1256, 1855, 2135, 2157, 2341, 2918
RseI CAYNNNNRTG 1 cut(s) 1470
SacI GAGCTC 1 cut(s) 1543
SatI GCNGC 5 cut(s) 655, 713, 934, 1524, 1904
Sau96I GGNCC 5 cut(s) 401, 887, 1085, 1108, 1990
ScaI AGTACT 1 cut(s) 2158
SchI GAGTC 4 cut(s) 467, 1423, 2432, 2753
ScrFI CCNGG 3 cut(s) 173, 1058, 2223
SduI GDGCHC 4 cut(s) 136, 418, 1376, 1543
SfaNI GCATC 9 cut(s) 470, 688, 754, 1036, 1462, 1535, 1968, 2318, 2337
SfcI CTRYAG 1 cut(s) 924
Sfr274I CTCGAG 1 cut(s) 891
SinI GGWCC 4 cut(s) 401, 1085, 1108, 1990
SlaI CTCGAG 1 cut(s) 891
SmiMI CAYNNNNRTG 1 cut(s) 1470
SmlI CTYRAG 2 cut(s) 260, 891
SmoI CTYRAG 2 cut(s) 260, 891
SseBI AGGCCT 1 cut(s) 246
SsiI CCGC 4 cut(s) 654, 1122, 1253, 2585
SspI AATATT 1 cut(s) 1808
SspMI CTAG 8 cut(s) 344, 626, 983, 1194, 1569, 1781, 2019, 2073
SstI GAGCTC 1 cut(s) 1543
StuI AGGCCT 1 cut(s) 246
StyD4I CCNGG 3 cut(s) 171, 1056, 2221
StyI CCWWGG 3 cut(s) 1868, 1993, 2785
TaaI ACNGT 6 cut(s) 390, 562, 649, 901, 1225, 2877
TaiI ACGT 5 cut(s) 615, 1663, 1708, 2130, 2475
TaqI TCGA 7 cut(s) 778, 892, 1489, 1561, 1876, 2489, 2848
TaqII GACCGA 1 cut(s) 1626
TatI WGTACW 5 cut(s) 618, 1854, 2134, 2156, 2340
TauI GCSGC 1 cut(s) 657
TfiI GAWTC 6 cut(s) 425, 739, 2207, 2299, 2609, 2845
TscAI CASTG 5 cut(s) 906, 1228, 1348, 1701, 2714
TseFI GTSAC 3 cut(s) 1616, 1726, 1840
TseI GCWGC 4 cut(s) 712, 933, 1523, 1903
Tsp45I GTSAC 3 cut(s) 1616, 1726, 1840
TspGWI ACGGA 1 cut(s) 1052
TspRI CASTG 5 cut(s) 906, 1228, 1348, 1701, 2714
Tth111I GACNNNGTC 1 cut(s) 1106
Van91I CCANNNNNTGG 1 cut(s) 1439
VpaK11BI GGWCC 4 cut(s) 401, 1085, 1108, 1990
VspI ATTAAT 2 cut(s) 554, 2727
XapI RAATTY 7 cut(s) 304, 540, 1186, 1400, 1502, 2163, 2480
XbaI TCTAGA 1 cut(s) 1568
XceI RCATGY 4 cut(s) 105, 708, 1334, 1739
XhoI CTCGAG 1 cut(s) 891
XmiI GTMKAC 1 cut(s) 1551
XmnI GAANNNNTTC 3 cut(s) 998, 1301, 1364
XspI CTAG 8 cut(s) 344, 626, 983, 1194, 1569, 1781, 2019, 2073
ZraI GACGTC 1 cut(s) 2473
ZrmI AGTACT 1 cut(s) 2158
Zsp2I ATGCAT 1 cut(s) 2311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.