pycom03g09490

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000321
Physical Location & Seq
Reverse (-)
8061551 .. 8062325
775 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g09490.1

Sequence Viewer

Length: 552 bp
ATGGAATTCCACTTGGAAGATGCTCTTCTTCCAATACCTAGTTGTGAAGCTGTGAATATCAAACAAGCAATTGGGAGTCACGTAGCTTGGCCACGACATCTTGTCGTGATAAATGATGAGTTGAGAACGAATCCACCCCTCAGGCAAAGATCCCAAAAACAACCTTTGGCTTTGCGTTCACTTATGCTCATAGCTCAAAACATGCATAAGGATGTTACTGTACCTATCATTATGGAATCTGACCTATTCGGAAATGAGCACACAACATTTATTGGTGGAGATGACATCATTCAATTTTGTTCCATGGCTGAAATATCGACTGTTTGCATTTCAATTTACATCAGGCAACTTTGGTCAACGTTGAAGAAGAACAATCTTGATGGAATGTTTGGATTTGTAGATCCTGGCAGAATTTCCCAGAAGGCTGGCAAAAAGGAACAAAGATCAAATGCACTGGCTCTTAGATTACAGAACTGCAAAAAAGGACAATTAATTTTTGCTCCGTATAACAAAGGGTTATTAGTACTATGTGTTTTGAACTTTTGTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 Transposase_24

Protein Analysis

184

Amino Acids

20.59

Weight (kDa)

8.78

Isoelectric Point (pI)

46.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000321)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221
prunus_persica Prupe.6G157700_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1
pyrus_communis pycom03g09490 pycom03g19580 pycom07g21940 pycom07g27440 pycom14g09710
rosa_chinensis RchiOBHm_Chr1g0318231 RchiOBHm_Chr1g0318241 RchiOBHm_Chr1g0325001 RchiOBHm_Chr1g0325021 RchiOBHm_Chr2g0112301 RchiOBHm_Chr3g0482281 RchiOBHm_Chr3g0482291 RchiOBHm_Chr4g0390561 RchiOBHm_Chr4g0390571 RchiOBHm_Chr4g0417951 RchiOBHm_Chr7g0232611 RchiOBHm_Chr7g0240601
rosa_laevigata RLG00000001069 RLG00000013674 RLG00000018830 RLG00000020747
rosa_multiflora Rmu_co8364947.1_g000001 Rmu_co8416715.1_g000001 Rmu_sc0001348.1_g000014 Rmu_sc0002986.1_g000026 Rmu_sc0004003.1_g000007 Rmu_sc0005137.1_g000014 Rmu_sc0006583.1_g000016
rosa_roxburghii Rroxscaffold_159G00432800 Rroxscaffold_174G00435170 Rroxscaffold_1G00001870 Rroxscaffold_1G00044480 Rroxscaffold_2G00111880 Rroxscaffold_2G00111890 Rroxscaffold_2G00119670 Rroxscaffold_2G00129450 Rroxscaffold_2G00131520 Rroxscaffold_3G00240880 Rroxscaffold_5G00337440 Rroxscaffold_5G00356220 Rroxscaffold_5G00362390 Rroxscaffold_5G00368260 Rroxscaffold_7G00196230 Rroxscaffold_7G00196240 Rroxscaffold_7G00202020
rosa_rugosa Rorug01G0094700 Rorug02G0050500 Rorug02G0179800 Rorug05G0042800 Rorug05G0043000 Rorug05G0043100 Rorug05G0043200 Rorug06G0045300 Rorug06G0045600 Rorug06G0069400 Rorug07G0213000 Rorug07G0213000
rosa_samantha Rh1AG031600 Rh1DG131700 Rh1DG134800 Rh1DG288000 Rh1DG288100 Rh2AG218500 Rh2AG232500 Rh2BG245900 Rh2CG236700 Rh2DG224300 Rh2DG224400 Rh2DG240200 Rh3AG247300 Rh3BG126600 Rh3BG126700 Rh3BG333600 Rh5AG440500 Rh5AG440600 Rh5BG457800 Rh5DG472700 Rh5DG472800 Rh6BG138000 Rh6BG138100 Rh6BG171500 Rh6BG188400 Rh6BG230000 Rh6CG135600 Rh6CG135700 Rh6CG186300 Rh6CG450300 Rh7AG376100 Rh7CG394800 Rh7CG426100 Rh7CG426200 Rh7DG247700 Rh7DG322600 Rh7DG334300 Rh7DG364700
rosa_wichuraiana Rw2G018040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 359
AclWI GGATC 2 cut(s) 144, 395
AcoI YGGCCR 1 cut(s) 89
AcsI RAATTY 2 cut(s) 5, 411
AfaI GTAC 2 cut(s) 222, 525
AgsI TTSAA 4 cut(s) 293, 333, 364, 538
AhdI GACNNNNNGTC 1 cut(s) 101
AjnI CCWGG 1 cut(s) 403
AloI GAACNNNNNNTCC 1 cut(s) 530
AluBI AGCT 3 cut(s) 50, 86, 194
AluI AGCT 3 cut(s) 50, 86, 194
Alw21I GWGCWC 1 cut(s) 261
AlwI GGATC 2 cut(s) 144, 395
AoxI GGCC 1 cut(s) 89
ApoI RAATTY 2 cut(s) 5, 411
AseI ATTAAT 1 cut(s) 491
AxyI CCTNAGG 1 cut(s) 140
BalI TGGCCA 1 cut(s) 91
Bbv12I GWGCWC 1 cut(s) 261
BccI CCATC 1 cut(s) 374
BciT130I CCWGG 1 cut(s) 405
BfaI CTAG 1 cut(s) 39
BmcAI AGTACT 1 cut(s) 525
Bme1390I CCNGG 1 cut(s) 405
BmeRI GACNNNNNGTC 1 cut(s) 101
BmrFI CCNGG 1 cut(s) 405
BmsI GCATC 1 cut(s) 10
BsaAI YACGTR 1 cut(s) 82
BsaJI CCNNGG 1 cut(s) 303
Bse1I ACTGG 1 cut(s) 459
Bse21I CCTNAGG 1 cut(s) 140
BseBI CCWGG 1 cut(s) 405
BseDI CCNNGG 1 cut(s) 303
BseGI GGATG 1 cut(s) 217
BseMII CTCAG 1 cut(s) 154
BseNI ACTGG 1 cut(s) 459
BshFI GGCC 1 cut(s) 91
BsiHKAI GWGCWC 1 cut(s) 261
BsnI GGCC 1 cut(s) 91
Bsp1286I GDGCHC 1 cut(s) 261
Bsp143I GATC 3 cut(s) 149, 400, 443
Bsp19I CCATGG 1 cut(s) 303
BspANI GGCC 1 cut(s) 91
BspCNI CTCAG 1 cut(s) 153
BspPI GGATC 2 cut(s) 144, 395
BspQI GCTCTTC 1 cut(s) 30
BsrI ACTGG 1 cut(s) 459
BssECI CCNNGG 1 cut(s) 303
BssMI GATC 3 cut(s) 149, 400, 443
BssT1I CCWWGG 1 cut(s) 303
Bst2UI CCWGG 1 cut(s) 405
Bst4CI ACNGT 2 cut(s) 220, 322
Bst6I CTCTTC 1 cut(s) 30
BstBAI YACGTR 1 cut(s) 82
BstC8I GCNNGC 1 cut(s) 427
BstDEI CTNAG 2 cut(s) 140, 461
BstDSI CCRYGG 1 cut(s) 303
BstF5I GGATG 1 cut(s) 217
BstKTI GATC 3 cut(s) 152, 403, 446
BstMBI GATC 3 cut(s) 149, 400, 443
BstNI CCWGG 1 cut(s) 405
BstNSI RCATGY 1 cut(s) 205
BstSCI CCNGG 1 cut(s) 403
BstX2I RGATCY 2 cut(s) 149, 400
BstXI CCANNNNNNTGG 1 cut(s) 425
BstYI RGATCY 2 cut(s) 149, 400
Bsu36I CCTNAGG 1 cut(s) 140
BsuRI GGCC 1 cut(s) 91
BtgI CCRYGG 1 cut(s) 303
BtsCI GGATG 1 cut(s) 217
BtsIMutI CAGTG 1 cut(s) 452
Cac8I GCNNGC 1 cut(s) 427
Csp6I GTAC 2 cut(s) 221, 524
CviAII CATG 2 cut(s) 202, 304
CviJI RGCY 8 cut(s) 50, 86, 91, 170, 194, 308, 425, 458
CviKI_1 RGCY 8 cut(s) 50, 86, 91, 170, 194, 308, 425, 458
CviQI GTAC 2 cut(s) 221, 524
DdeI CTNAG 2 cut(s) 140, 461
DpnI GATC 3 cut(s) 151, 402, 445
DpnII GATC 3 cut(s) 149, 400, 443
DriI GACNNNNNGTC 1 cut(s) 101
EaeI YGGCCR 1 cut(s) 89
Eam1104I CTCTTC 1 cut(s) 30
Eam1105I GACNNNNNGTC 1 cut(s) 101
EarI CTCTTC 1 cut(s) 30
Eco130I CCWWGG 1 cut(s) 303
Eco81I CCTNAGG 1 cut(s) 140
EcoRI GAATTC 1 cut(s) 5
EcoRII CCWGG 1 cut(s) 403
EcoT14I CCWWGG 1 cut(s) 303
EcoT22I ATGCAT 1 cut(s) 207
ErhI CCWWGG 1 cut(s) 303
FaeI CATG 2 cut(s) 205, 307
FaiI YATR 8 cut(s) 185, 191, 203, 207, 233, 305, 507, 529
FalI AAGNNNNNCTT 2 cut(s) 9, 41
FatI CATG 2 cut(s) 201, 303
FokI GGATG 1 cut(s) 224
FspBI CTAG 1 cut(s) 39
HaeIII GGCC 1 cut(s) 91
Hin1II CATG 2 cut(s) 205, 307
HincII GTYRAC 1 cut(s) 357
HindII GTYRAC 1 cut(s) 357
HinfI GANTC 3 cut(s) 76, 130, 236
Hpy166II GTNNAC 2 cut(s) 179, 357
Hpy188I TCNGA 2 cut(s) 241, 251
Hpy188III TCNNGA 2 cut(s) 106, 377
Hpy8I GTNNAC 2 cut(s) 179, 357
HpyAV CCTTC 1 cut(s) 415
HpyCH4III ACNGT 2 cut(s) 220, 322
HpyCH4IV ACGT 2 cut(s) 81, 359
HpyCH4V TGCA 4 cut(s) 205, 327, 452, 477
HpyF3I CTNAG 2 cut(s) 140, 461
HpySE526I ACGT 2 cut(s) 81, 359
Hsp92II CATG 2 cut(s) 205, 307
Kzo9I GATC 3 cut(s) 149, 400, 443
LguI GCTCTTC 1 cut(s) 30
LmnI GCTCC 1 cut(s) 505
LpnPI CCDG 7 cut(s) 127, 328, 390, 411, 417, 431, 440
LweI GCATC 1 cut(s) 10
MaeI CTAG 1 cut(s) 39
MaeII ACGT 2 cut(s) 81, 359
MaeIII GTNAC 2 cut(s) 77, 214
MalI GATC 3 cut(s) 151, 402, 445
MboI GATC 3 cut(s) 149, 400, 443
MboII GAAGA 5 cut(s) 17, 20, 29, 376, 379
MfeI CAATTG 1 cut(s) 69
MflI RGATCY 2 cut(s) 149, 400
MhlI GDGCHC 1 cut(s) 261
MlsI TGGCCA 1 cut(s) 91
MluCI AATT 7 cut(s) 5, 69, 293, 333, 411, 488, 492
MluNI TGGCCA 1 cut(s) 91
MlyI GAGTC 1 cut(s) 85
MnlI CCTC 1 cut(s) 149
Mox20I TGGCCA 1 cut(s) 91
Mph1103I ATGCAT 1 cut(s) 207
MscI TGGCCA 1 cut(s) 91
MseI TTAA 1 cut(s) 491
MslI CAYNNNNRTG 1 cut(s) 210
Msp20I TGGCCA 1 cut(s) 91
MspR9I CCNGG 1 cut(s) 405
MunI CAATTG 1 cut(s) 69
MvaI CCWGG 1 cut(s) 405
NcoI CCATGG 1 cut(s) 303
NdeII GATC 3 cut(s) 149, 400, 443
NlaIII CATG 2 cut(s) 205, 307
NmuCI GTSAC 1 cut(s) 77
NsiI ATGCAT 1 cut(s) 207
NspI RCATGY 1 cut(s) 205
PciSI GCTCTTC 1 cut(s) 30
PfeI GAWTC 2 cut(s) 130, 236
PleI GAGTC 1 cut(s) 84
PpsI GAGTC 1 cut(s) 84
Ppu21I YACGTR 1 cut(s) 82
PshBI ATTAAT 1 cut(s) 491
Psp1406I AACGTT 1 cut(s) 359
Psp6I CCWGG 1 cut(s) 403
PspGI CCWGG 1 cut(s) 403
PsuI RGATCY 2 cut(s) 149, 400
RsaI GTAC 2 cut(s) 222, 525
RsaNI GTAC 2 cut(s) 221, 524
RseI CAYNNNNRTG 1 cut(s) 210
SapI GCTCTTC 1 cut(s) 30
SaqAI TTAA 1 cut(s) 491
Sau3AI GATC 3 cut(s) 149, 400, 443
ScaI AGTACT 1 cut(s) 525
SchI GAGTC 1 cut(s) 85
ScrFI CCNGG 1 cut(s) 405
SduI GDGCHC 1 cut(s) 261
SetI ASST 9 cut(s) 40, 52, 84, 88, 166, 196, 226, 246, 362
SfaNI GCATC 1 cut(s) 10
SmiMI CAYNNNNRTG 1 cut(s) 210
Sse9I AATT 7 cut(s) 5, 69, 293, 333, 411, 488, 492
SspMI CTAG 1 cut(s) 39
StyD4I CCNGG 1 cut(s) 403
StyI CCWWGG 1 cut(s) 303
TaaI ACNGT 2 cut(s) 220, 322
TaiI ACGT 2 cut(s) 84, 362
TaqI TCGA 1 cut(s) 317
TasI AATT 7 cut(s) 5, 69, 293, 333, 411, 488, 492
TatI WGTACW 1 cut(s) 523
TfiI GAWTC 2 cut(s) 130, 236
Tru1I TTAA 1 cut(s) 491
Tru9I TTAA 1 cut(s) 491
TscAI CASTG 1 cut(s) 459
TseFI GTSAC 1 cut(s) 77
Tsp45I GTSAC 1 cut(s) 77
TspGWI ACGGA 1 cut(s) 492
TspRI CASTG 1 cut(s) 459
VspI ATTAAT 1 cut(s) 491
XapI RAATTY 2 cut(s) 5, 411
XceI RCATGY 1 cut(s) 205
XspI CTAG 1 cut(s) 39
ZrmI AGTACT 1 cut(s) 525
Zsp2I ATGCAT 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.