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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1410 |
| Acc36I | ACCTGC | 1 cut(s) | 1747 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 84 |
| AccI | GTMKAC | 2 cut(s) | 483, 774 |
| AciI | CCGC | 3 cut(s) | 561, 1312, 1751 |
| AclWI | GGATC | 9 cut(s) | 389, 402, 404, 417, 466, 1290, 1298, 1311, 1621 |
| AcoI | YGGCCR | 1 cut(s) | 1309 |
| AcsI | RAATTY | 1 cut(s) | 927 |
| AfaI | GTAC | 3 cut(s) | 293, 955, 1646 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 84 |
| AflIII | ACRYGT | 3 cut(s) | 375, 438, 1446 |
| AgeI | ACCGGT | 1 cut(s) | 950 |
| AgsI | TTSAA | 2 cut(s) | 1267, 1775 |
| AjnI | CCWGG | 3 cut(s) | 1306, 1501, 1609 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1134, 1166 |
| AluBI | AGCT | 9 cut(s) | 227, 470, 557, 766, 923, 947, 1328, 1525, 1677 |
| AluI | AGCT | 9 cut(s) | 227, 470, 557, 766, 923, 947, 1328, 1525, 1677 |
| Alw21I | GWGCWC | 1 cut(s) | 1016 |
| Alw26I | GTCTC | 8 cut(s) | 122, 227, 429, 655, 722, 1240, 1569, 1700 |
| AlwI | GGATC | 9 cut(s) | 389, 402, 404, 417, 466, 1290, 1298, 1311, 1621 |
| Ama87I | CYCGRG | 1 cut(s) | 398 |
| AoxI | GGCC | 3 cut(s) | 250, 968, 1309 |
| ApeKI | GCWGC | 4 cut(s) | 1052, 1210, 1436, 1674 |
| ApoI | RAATTY | 1 cut(s) | 927 |
| AsiGI | ACCGGT | 1 cut(s) | 950 |
| AspA2I | CCTAGG | 1 cut(s) | 844 |
| AspLEI | GCGC | 1 cut(s) | 1357 |
| AspS9I | GGNCC | 1 cut(s) | 250 |
| AsuII | TTCGAA | 2 cut(s) | 574, 1738 |
| AsuNHI | GCTAGC | 1 cut(s) | 766 |
| AvaI | CYCGRG | 1 cut(s) | 398 |
| AvrII | CCTAGG | 1 cut(s) | 844 |
| BaeGI | GKGCMC | 2 cut(s) | 843, 882 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 945, 978 |
| BamHI | GGATCC | 3 cut(s) | 394, 409, 1303 |
| BbsI | GAAGAC | 2 cut(s) | 495, 1017 |
| Bbv12I | GWGCWC | 1 cut(s) | 1016 |
| BbvI | GCAGC | 4 cut(s) | 1064, 1222, 1423, 1686 |
| BccI | CCATC | 6 cut(s) | 86, 601, 815, 890, 914, 1757 |
| BceAI | ACGGC | 1 cut(s) | 237 |
| BciT130I | CCWGG | 3 cut(s) | 1308, 1503, 1611 |
| BcoDI | GTCTC | 8 cut(s) | 122, 227, 429, 655, 722, 1240, 1569, 1700 |
| BfaI | CTAG | 9 cut(s) | 6, 306, 422, 471, 767, 845, 1329, 1344, 1602 |
| BfmI | CTRYAG | 1 cut(s) | 1116 |
| BfuAI | ACCTGC | 1 cut(s) | 1747 |
| BisI | GCNGC | 5 cut(s) | 1053, 1211, 1312, 1437, 1675 |
| BlnI | CCTAGG | 1 cut(s) | 844 |
| BlsI | GCNGC | 5 cut(s) | 1054, 1212, 1313, 1438, 1676 |
| Bme1390I | CCNGG | 3 cut(s) | 1308, 1503, 1611 |
| BmeT110I | CYCGRG | 1 cut(s) | 398 |
| BmgT120I | GGNCC | 1 cut(s) | 250 |
| BmiI | GGNNCC | 3 cut(s) | 396, 411, 1305 |
| BmrFI | CCNGG | 3 cut(s) | 1308, 1503, 1611 |
| BmsI | GCATC | 8 cut(s) | 148, 348, 539, 883, 1053, 1087, 1197, 1323 |
| BmtI | GCTAGC | 1 cut(s) | 770 |
| BpiI | GAAGAC | 2 cut(s) | 495, 1017 |
| Bpu10I | CCTNAGC | 1 cut(s) | 240 |
| Bpu14I | TTCGAA | 2 cut(s) | 574, 1738 |
| BpuEI | CTTGAG | 2 cut(s) | 207, 1701 |
| BsaBI | GATNNNNATC | 2 cut(s) | 897, 1372 |
| BsaJI | CCNNGG | 1 cut(s) | 844 |
| BsaWI | WCCGGW | 1 cut(s) | 950 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 580, 610 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 84 |
| Bse118I | RCCGGY | 1 cut(s) | 950 |
| Bse1I | ACTGG | 2 cut(s) | 1195, 1574 |
| Bse3DI | GCAATG | 2 cut(s) | 804, 1162 |
| Bse8I | GATNNNNATC | 2 cut(s) | 897, 1372 |
| BseBI | CCWGG | 3 cut(s) | 1308, 1503, 1611 |
| BseDI | CCNNGG | 1 cut(s) | 844 |
| BseGI | GGATG | 5 cut(s) | 589, 826, 906, 1114, 1191 |
| BseJI | GATNNNNATC | 2 cut(s) | 897, 1372 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 84 |
| BseMI | GCAATG | 2 cut(s) | 804, 1162 |
| BseMII | CTCAG | 4 cut(s) | 231, 492, 525, 1355 |
| BseNI | ACTGG | 2 cut(s) | 1195, 1574 |
| BseRI | GAGGAG | 1 cut(s) | 684 |
| BseSI | GKGCMC | 2 cut(s) | 843, 882 |
| BseXI | GCAGC | 4 cut(s) | 1064, 1222, 1423, 1686 |
| BshFI | GGCC | 3 cut(s) | 252, 970, 1311 |
| BshTI | ACCGGT | 1 cut(s) | 950 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1016 |
| BsiHKCI | CYCGRG | 1 cut(s) | 398 |
| BsiSI | CCGG | 1 cut(s) | 951 |
| BslFI | GGGAC | 3 cut(s) | 67, 663, 1115 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 84 |
| BsmAI | GTCTC | 8 cut(s) | 122, 227, 429, 655, 722, 1240, 1569, 1700 |
| BsmFI | GGGAC | 3 cut(s) | 67, 663, 1115 |
| BsnI | GGCC | 3 cut(s) | 252, 970, 1311 |
| BsoBI | CYCGRG | 1 cut(s) | 398 |
| Bsp119I | TTCGAA | 2 cut(s) | 574, 1738 |
| Bsp1286I | GDGCHC | 3 cut(s) | 843, 882, 1016 |
| Bsp143I | GATC | 9 cut(s) | 369, 394, 409, 458, 1282, 1303, 1346, 1387, 1613 |
| BspACI | CCGC | 3 cut(s) | 561, 1312, 1751 |
| BspANI | GGCC | 3 cut(s) | 252, 970, 1311 |
| BspCNI | CTCAG | 4 cut(s) | 232, 491, 526, 1356 |
| BspLI | GGNNCC | 3 cut(s) | 396, 411, 1305 |
| BspMI | ACCTGC | 1 cut(s) | 1747 |
| BspOI | GCTAGC | 1 cut(s) | 770 |
| BspPI | GGATC | 9 cut(s) | 389, 402, 404, 417, 466, 1290, 1298, 1311, 1621 |
| BspT104I | TTCGAA | 2 cut(s) | 574, 1738 |
| BsrDI | GCAATG | 2 cut(s) | 804, 1162 |
| BsrFI | RCCGGY | 1 cut(s) | 950 |
| BsrI | ACTGG | 2 cut(s) | 1195, 1574 |
| BssAI | RCCGGY | 1 cut(s) | 950 |
| BssECI | CCNNGG | 1 cut(s) | 844 |
| BssMI | GATC | 9 cut(s) | 369, 394, 409, 458, 1282, 1303, 1346, 1387, 1613 |
| BssT1I | CCWWGG | 1 cut(s) | 844 |
| Bst2UI | CCWGG | 3 cut(s) | 1308, 1503, 1611 |
| Bst4CI | ACNGT | 4 cut(s) | 336, 808, 937, 1181 |
| Bst6I | CTCTTC | 3 cut(s) | 24, 993, 1716 |
| BstBI | TTCGAA | 2 cut(s) | 574, 1738 |
| BstC8I | GCNNGC | 3 cut(s) | 764, 768, 945 |
| BstDEI | CTNAG | 6 cut(s) | 240, 478, 507, 522, 534, 1364 |
| BstF5I | GGATG | 5 cut(s) | 589, 826, 906, 1114, 1191 |
| BstHHI | GCGC | 1 cut(s) | 1357 |
| BstKTI | GATC | 9 cut(s) | 372, 397, 412, 461, 1285, 1306, 1349, 1390, 1616 |
| BstMAI | GTCTC | 8 cut(s) | 122, 227, 429, 655, 722, 1240, 1569, 1700 |
| BstMBI | GATC | 9 cut(s) | 369, 394, 409, 458, 1282, 1303, 1346, 1387, 1613 |
| BstNI | CCWGG | 3 cut(s) | 1308, 1503, 1611 |
| BstNSI | RCATGY | 2 cut(s) | 379, 442 |
| BstSCI | CCNGG | 3 cut(s) | 1306, 1501, 1609 |
| BstSFI | CTRYAG | 1 cut(s) | 1116 |
| BstSLI | GKGCMC | 2 cut(s) | 843, 882 |
| BstV1I | GCAGC | 4 cut(s) | 1064, 1222, 1423, 1686 |
| BstV2I | GAAGAC | 2 cut(s) | 495, 1017 |
| BstX2I | RGATCY | 5 cut(s) | 394, 409, 458, 1303, 1613 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1581 |
| BstYI | RGATCY | 5 cut(s) | 394, 409, 458, 1303, 1613 |
| BsuRI | GGCC | 3 cut(s) | 252, 970, 1311 |
| BtsCI | GGATG | 5 cut(s) | 589, 826, 906, 1114, 1191 |
| BtsI | GCAGTG | 1 cut(s) | 1128 |
| BtsIMutI | CAGTG | 2 cut(s) | 1128, 1361 |
| BveI | ACCTGC | 1 cut(s) | 1747 |
| Cac8I | GCNNGC | 3 cut(s) | 764, 768, 945 |
| CfoI | GCGC | 1 cut(s) | 1357 |
| Cfr10I | RCCGGY | 1 cut(s) | 950 |
| Cfr13I | GGNCC | 1 cut(s) | 250 |
| Csp6I | GTAC | 3 cut(s) | 292, 954, 1645 |
| CspAI | ACCGGT | 1 cut(s) | 950 |
| CviQI | GTAC | 3 cut(s) | 292, 954, 1645 |
| DdeI | CTNAG | 6 cut(s) | 240, 478, 507, 522, 534, 1364 |
| DpnI | GATC | 9 cut(s) | 371, 396, 411, 460, 1284, 1305, 1348, 1389, 1615 |
| DpnII | GATC | 9 cut(s) | 369, 394, 409, 458, 1282, 1303, 1346, 1387, 1613 |
| DraI | TTTAAA | 1 cut(s) | 1686 |
| EaeI | YGGCCR | 1 cut(s) | 1309 |
| Eam1104I | CTCTTC | 3 cut(s) | 24, 993, 1716 |
| EarI | CTCTTC | 3 cut(s) | 24, 993, 1716 |
| EciI | GGCGGA | 1 cut(s) | 1766 |
| Eco130I | CCWWGG | 1 cut(s) | 844 |
| Eco88I | CYCGRG | 1 cut(s) | 398 |
| EcoRII | CCWGG | 3 cut(s) | 1306, 1501, 1609 |
| EcoT14I | CCWWGG | 1 cut(s) | 844 |
| EcoT22I | ATGCAT | 1 cut(s) | 1297 |
| ErhI | CCWWGG | 1 cut(s) | 844 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 278, 310 |
| FaqI | GGGAC | 3 cut(s) | 67, 663, 1115 |
| FblI | GTMKAC | 2 cut(s) | 483, 774 |
| Fnu4HI | GCNGC | 5 cut(s) | 1053, 1211, 1312, 1437, 1675 |
| FokI | GGATG | 5 cut(s) | 596, 833, 893, 1121, 1178 |
| Fsp4HI | GCNGC | 5 cut(s) | 1053, 1211, 1312, 1437, 1675 |
| FspBI | CTAG | 9 cut(s) | 6, 306, 422, 471, 767, 845, 1329, 1344, 1602 |
| GlaI | GCGC | 1 cut(s) | 1356 |
| GluI | GCNGC | 5 cut(s) | 1053, 1211, 1312, 1437, 1675 |
| HaeIII | GGCC | 3 cut(s) | 252, 970, 1311 |
| HapII | CCGG | 1 cut(s) | 951 |
| HhaI | GCGC | 1 cut(s) | 1357 |
| Hin6I | GCGC | 1 cut(s) | 1355 |
| HinP1I | GCGC | 1 cut(s) | 1355 |
| HincII | GTYRAC | 1 cut(s) | 484 |
| HindII | GTYRAC | 1 cut(s) | 484 |
| HindIII | AAGCTT | 1 cut(s) | 225 |
| HinfI | GANTC | 5 cut(s) | 430, 917, 1112, 1373, 1420 |
| HpaII | CCGG | 1 cut(s) | 951 |
| Hpy166II | GTNNAC | 5 cut(s) | 292, 484, 775, 1452, 1571 |
| Hpy188I | TCNGA | 3 cut(s) | 446, 463, 535 |
| Hpy188III | TCNNGA | 4 cut(s) | 306, 398, 434, 1718 |
| Hpy8I | GTNNAC | 5 cut(s) | 292, 484, 775, 1452, 1571 |
| HpyAV | CCTTC | 2 cut(s) | 619, 1136 |
| HpyCH4III | ACNGT | 4 cut(s) | 336, 808, 937, 1181 |
| HpyCH4IV | ACGT | 2 cut(s) | 142, 1448 |
| HpyCH4V | TGCA | 9 cut(s) | 320, 361, 762, 797, 1044, 1078, 1210, 1230, 1295 |
| HpyF3I | CTNAG | 6 cut(s) | 240, 478, 507, 522, 534, 1364 |
| HpySE526I | ACGT | 2 cut(s) | 142, 1448 |
| HspAI | GCGC | 1 cut(s) | 1355 |
| Kzo9I | GATC | 9 cut(s) | 369, 394, 409, 458, 1282, 1303, 1346, 1387, 1613 |
| LmnI | GCTCC | 3 cut(s) | 671, 848, 1753 |
| Lsp1109I | GCAGC | 4 cut(s) | 1064, 1222, 1423, 1686 |
| LweI | GCATC | 8 cut(s) | 148, 348, 539, 883, 1053, 1087, 1197, 1323 |
| MaeI | CTAG | 9 cut(s) | 6, 306, 422, 471, 767, 845, 1329, 1344, 1602 |
| MaeII | ACGT | 2 cut(s) | 142, 1448 |
| MaeIII | GTNAC | 5 cut(s) | 113, 277, 990, 1181, 1484 |
| MalI | GATC | 9 cut(s) | 371, 396, 411, 460, 1284, 1305, 1348, 1389, 1615 |
| MboI | GATC | 9 cut(s) | 369, 394, 409, 458, 1282, 1303, 1346, 1387, 1613 |
| MfeI | CAATTG | 3 cut(s) | 600, 635, 870 |
| MflI | RGATCY | 5 cut(s) | 394, 409, 458, 1303, 1613 |
| MhlI | GDGCHC | 3 cut(s) | 843, 882, 1016 |
| MlyI | GAGTC | 1 cut(s) | 926 |
| MmeI | TCCRAC | 4 cut(s) | 318, 330, 387, 798 |
| Mph1103I | ATGCAT | 1 cut(s) | 1297 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1290, 1535 |
| MspA1I | CMGCKG | 1 cut(s) | 923 |
| MspI | CCGG | 1 cut(s) | 951 |
| MspR9I | CCNGG | 3 cut(s) | 1308, 1503, 1611 |
| MunI | CAATTG | 3 cut(s) | 600, 635, 870 |
| MvaI | CCWGG | 3 cut(s) | 1308, 1503, 1611 |
| NdeII | GATC | 9 cut(s) | 369, 394, 409, 458, 1282, 1303, 1346, 1387, 1613 |
| NheI | GCTAGC | 1 cut(s) | 766 |
| NlaIV | GGNNCC | 3 cut(s) | 396, 411, 1305 |
| NmuCI | GTSAC | 1 cut(s) | 990 |
| NsiI | ATGCAT | 1 cut(s) | 1297 |
| NspI | RCATGY | 2 cut(s) | 379, 442 |
| NspV | TTCGAA | 2 cut(s) | 574, 1738 |
| PciI | ACATGT | 2 cut(s) | 375, 438 |
| PfeI | GAWTC | 4 cut(s) | 430, 1112, 1373, 1420 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 84 |
| PinAI | ACCGGT | 1 cut(s) | 950 |
| PkrI | GCNGC | 5 cut(s) | 1054, 1212, 1313, 1438, 1676 |
| PleI | GAGTC | 1 cut(s) | 925 |
| PpsI | GAGTC | 1 cut(s) | 925 |
| PscI | ACATGT | 2 cut(s) | 375, 438 |
| PsiI | TTATAA | 1 cut(s) | 1410 |
| Psp6I | CCWGG | 3 cut(s) | 1306, 1501, 1609 |
| PspGI | CCWGG | 3 cut(s) | 1306, 1501, 1609 |
| PspN4I | GGNNCC | 3 cut(s) | 396, 411, 1305 |
| PspPI | GGNCC | 1 cut(s) | 250 |
| PsuI | RGATCY | 5 cut(s) | 394, 409, 458, 1303, 1613 |
| PvuII | CAGCTG | 1 cut(s) | 923 |
| RsaI | GTAC | 3 cut(s) | 293, 955, 1646 |
| RsaNI | GTAC | 3 cut(s) | 292, 954, 1645 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1290, 1535 |
| SalI | GTCGAC | 1 cut(s) | 482 |
| SatI | GCNGC | 5 cut(s) | 1053, 1211, 1312, 1437, 1675 |
| Sau3AI | GATC | 9 cut(s) | 369, 394, 409, 458, 1282, 1303, 1346, 1387, 1613 |
| Sau96I | GGNCC | 1 cut(s) | 250 |
| SchI | GAGTC | 1 cut(s) | 926 |
| ScrFI | CCNGG | 3 cut(s) | 1308, 1503, 1611 |
| SduI | GDGCHC | 3 cut(s) | 843, 882, 1016 |
| SfaNI | GCATC | 8 cut(s) | 148, 348, 539, 883, 1053, 1087, 1197, 1323 |
| SfcI | CTRYAG | 1 cut(s) | 1116 |
| SfuI | TTCGAA | 2 cut(s) | 574, 1738 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1290, 1535 |
| SmlI | CTYRAG | 2 cut(s) | 222, 1716 |
| SmoI | CTYRAG | 2 cut(s) | 222, 1716 |
| SsiI | CCGC | 3 cut(s) | 561, 1312, 1751 |
| SspMI | CTAG | 9 cut(s) | 6, 306, 422, 471, 767, 845, 1329, 1344, 1602 |
| StyD4I | CCNGG | 3 cut(s) | 1306, 1501, 1609 |
| StyI | CCWWGG | 1 cut(s) | 844 |
| TaaI | ACNGT | 4 cut(s) | 336, 808, 937, 1181 |
| TaiI | ACGT | 2 cut(s) | 145, 1451 |
| TatI | WGTACW | 2 cut(s) | 291, 1644 |
| TauI | GCSGC | 1 cut(s) | 1314 |
| TfiI | GAWTC | 4 cut(s) | 430, 1112, 1373, 1420 |
| TscAI | CASTG | 2 cut(s) | 1128, 1368 |
| TseFI | GTSAC | 1 cut(s) | 990 |
| TseI | GCWGC | 4 cut(s) | 1052, 1210, 1436, 1674 |
| Tsp45I | GTSAC | 1 cut(s) | 990 |
| TspDTI | ATGAA | 9 cut(s) | 375, 1011, 1125, 1162, 1338, 1523, 1568, 1655, 1664 |
| TspGWI | ACGGA | 1 cut(s) | 1063 |
| TspRI | CASTG | 2 cut(s) | 1128, 1368 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 84 |
| XapI | RAATTY | 1 cut(s) | 927 |
| XbaI | TCTAGA | 1 cut(s) | 305 |
| XceI | RCATGY | 2 cut(s) | 379, 442 |
| XmaJI | CCTAGG | 1 cut(s) | 844 |
| XmiI | GTMKAC | 2 cut(s) | 483, 774 |
| XspI | CTAG | 9 cut(s) | 6, 306, 422, 471, 767, 845, 1329, 1344, 1602 |
| Zsp2I | ATGCAT | 1 cut(s) | 1297 |