Rh6BG188400

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000321
Physical Location & Seq
Reverse (-)
34963370 .. 34966136
2767 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG188400.1

Sequence Viewer

Length: 1374 bp
ATGGATCCAACTCTCATGTCCGACATTATTCATGGTAGGAGTAAGGGGGCTCGGATGGAAGTTACTTATAGTAAAAAAGGGCAACCAATTGGTCTTGGAGGGAAGAGACTAGCAACTTTTATTGGGGTAATGGCTCAAACTACTATCCCAATTACATATGAGACTTGGCCAACCGTGAAGAATTCACTTAAAGAAATGATATGGAGTATGGTTCAGAAATCATTCATTGTGGATCCAAGAAGCAAGAAAGATGTCTTGAGTAGTGCAGGAAGGAAATGGAAATCATTCAAGAGCACTTTAACTACTAAATATATATTGAAGTATAAAGATCGACGGAGCCTCCTAAAGAAAAAACTTGAAGAATATGAGTTCATTACTCAACCTGAGTGGGAAGCTTTTGTCAAATCTCGATTAACTCCTGAATTTTTGGAAACTCATGAGGACCACTCAAGGAGACGAGCTTTGCATGAGTATGATCATCGAATGTCCAGGAAAGGCTATGCTAATTTGGAGGAGGAACTAAAAATGGAGTTAGGGACTAAAGAAGATATCGATAGAGCCATCTTATGGAAGAAAGGGCATGTAAATAAAGAGGGTAACTATTTGAGTGAGACAACCAAACAACGTGCTGAGAAAATTGCAAAACAACAGTACAAAGGGGGACTAATAATGAGAGAGATAGAAGAATTGGAAGATGACAATGTGATAACATCAACAGAGAAAGCATATATTCCTATTGAGACAATAAATGTTGTAAAAAAGAGAAAAAAATGCAAGCTAGCAGTAGACAGCACAGATAACATCGTTGCAATGGGAACCGTAATAATGTTGGATGGGCCAATACATGGAGTGCCCCTAGGAGCACAAAATGTACATATCTCAATTGATGTGTCTATCAAGGAGGATGCTTATCTCCCAATCCCAAATGTGTCAGGTGACATTTTTACAGTAAAGCAAGCCATCGGTACTCATGTTGCTTGGCCTCGACATCTTGTTTTAATGTCACATGAAGAGAACTCAATGAGCACTCAAAAGTTTTTCAGTAAAACACCATGTAGACAAGCAGCATACAAGATGCCTGTATCTGTGCATCTTCTACTGCGTTTGGCAAGAACAATGGATGAATCTATAGCAGTGTTGGTCCCCATGGAAGATGGTGTGTTTGGCAATGACCACAATACATTCATAAACAATAACGACATCATCCATTTTTGTTTGATGCAACCAATATCCACCATTTGCATTTCTATCTACATGAGACACCTCTGGTCATTGTTGAAAATGAAGGATGAGGATCACTTGTATGCATTTGTGGATCCTGGCCGCATCTCTAATGAAGCTGGAAAGGAATCATTGGTTGTTGGCTGCCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 Transposase_24

Protein Analysis

457

Amino Acids

52.25

Weight (kDa)

8.95

Isoelectric Point (pI)

44.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transposase_24 PF03004 130 - 216 5.3e-09 Plant transposase (Ptta/En/Spm family)
DUF8039 PF26133 250 - 334 1.2e-13 Domain of unknown function (DUF8039)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000321)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221
prunus_persica Prupe.6G157700_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1
pyrus_communis pycom03g09490 pycom03g19580 pycom07g21940 pycom07g27440 pycom14g09710
rosa_chinensis RchiOBHm_Chr1g0318231 RchiOBHm_Chr1g0318241 RchiOBHm_Chr1g0325001 RchiOBHm_Chr1g0325021 RchiOBHm_Chr2g0112301 RchiOBHm_Chr3g0482281 RchiOBHm_Chr3g0482291 RchiOBHm_Chr4g0390561 RchiOBHm_Chr4g0390571 RchiOBHm_Chr4g0417951 RchiOBHm_Chr7g0232611 RchiOBHm_Chr7g0240601
rosa_laevigata RLG00000001069 RLG00000013674 RLG00000018830 RLG00000020747
rosa_multiflora Rmu_co8364947.1_g000001 Rmu_co8416715.1_g000001 Rmu_sc0001348.1_g000014 Rmu_sc0002986.1_g000026 Rmu_sc0004003.1_g000007 Rmu_sc0005137.1_g000014 Rmu_sc0006583.1_g000016
rosa_roxburghii Rroxscaffold_159G00432800 Rroxscaffold_174G00435170 Rroxscaffold_1G00001870 Rroxscaffold_1G00044480 Rroxscaffold_2G00111880 Rroxscaffold_2G00111890 Rroxscaffold_2G00119670 Rroxscaffold_2G00129450 Rroxscaffold_2G00131520 Rroxscaffold_3G00240880 Rroxscaffold_5G00337440 Rroxscaffold_5G00356220 Rroxscaffold_5G00362390 Rroxscaffold_5G00368260 Rroxscaffold_7G00196230 Rroxscaffold_7G00196240 Rroxscaffold_7G00202020
rosa_rugosa Rorug01G0094700 Rorug02G0050500 Rorug02G0179800 Rorug05G0042800 Rorug05G0043000 Rorug05G0043100 Rorug05G0043200 Rorug06G0045300 Rorug06G0045600 Rorug06G0069400 Rorug07G0213000 Rorug07G0213000
rosa_samantha Rh1AG031600 Rh1DG131700 Rh1DG134800 Rh1DG288000 Rh1DG288100 Rh2AG218500 Rh2AG232500 Rh2BG245900 Rh2CG236700 Rh2DG224300 Rh2DG224400 Rh2DG240200 Rh3AG247300 Rh3BG126600 Rh3BG126700 Rh3BG333600 Rh5AG440500 Rh5AG440600 Rh5BG457800 Rh5DG472700 Rh5DG472800 Rh6BG138000 Rh6BG138100 Rh6BG171500 Rh6BG188400 Rh6BG230000 Rh6CG135600 Rh6CG135700 Rh6CG186300 Rh6CG450300 Rh7AG376100 Rh7CG394800 Rh7CG426100 Rh7CG426200 Rh7DG247700 Rh7DG322600 Rh7DG334300 Rh7DG364700
rosa_wichuraiana Rw2G018040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 567, 845
AccI GTMKAC 2 cut(s) 786, 1057
AciI CCGC 1 cut(s) 1324
AclWI GGATC 6 cut(s) 12, 227, 240, 1302, 1310, 1323
AcoI YGGCCR 2 cut(s) 167, 1321
AcsI RAATTY 2 cut(s) 181, 422
AfaI GTAC 3 cut(s) 653, 873, 967
AfiI CCNNNNNNNGG 2 cut(s) 567, 845
AgsI TTSAA 4 cut(s) 289, 319, 359, 1279
AjnI CCWGG 2 cut(s) 488, 1318
AluBI AGCT 4 cut(s) 395, 461, 778, 1340
AluI AGCT 4 cut(s) 395, 461, 778, 1340
Alw21I GWGCWC 3 cut(s) 296, 865, 1028
Alw26I GTCTC 6 cut(s) 100, 155, 448, 605, 734, 1252
AlwI GGATC 6 cut(s) 12, 227, 240, 1302, 1310, 1323
AoxI GGCC 4 cut(s) 167, 836, 980, 1321
ApeKI GCWGC 2 cut(s) 1064, 1365
ApoI RAATTY 2 cut(s) 181, 422
Asp700I GAANNNNTTC 2 cut(s) 221, 284
AspA2I CCTAGG 1 cut(s) 856
AspS9I GGNCC 3 cut(s) 442, 836, 1141
AsuHPI GGTGA 1 cut(s) 947
AsuNHI GCTAGC 1 cut(s) 778
AvaII GGWCC 2 cut(s) 442, 1141
AvrII CCTAGG 1 cut(s) 856
BaeGI GKGCMC 1 cut(s) 855
BaeI ACNNNNGTAYC 2 cut(s) 957, 990
BalI TGGCCA 1 cut(s) 169
BamHI GGATCC 3 cut(s) 4, 232, 1315
BanII GRGCYC 1 cut(s) 52
Bbv12I GWGCWC 3 cut(s) 296, 865, 1028
BbvI GCAGC 2 cut(s) 1076, 1352
BccI CCATC 5 cut(s) 49, 569, 827, 968, 1148
BciT130I CCWGG 2 cut(s) 490, 1320
BclI TGATCA 1 cut(s) 475
BcoDI GTCTC 6 cut(s) 100, 155, 448, 605, 734, 1252
BfaI CTAG 3 cut(s) 110, 779, 857
BfmI CTRYAG 1 cut(s) 1128
BisI GCNGC 3 cut(s) 1065, 1324, 1366
BlnI CCTAGG 1 cut(s) 856
BlsI GCNGC 3 cut(s) 1066, 1325, 1367
Bme1390I CCNGG 2 cut(s) 490, 1320
Bme18I GGWCC 2 cut(s) 442, 1141
BmgT120I GGNCC 3 cut(s) 442, 836, 1141
BmiI GGNNCC 6 cut(s) 6, 234, 338, 817, 1143, 1317
BmrFI CCNGG 2 cut(s) 490, 1320
BmsI GCATC 5 cut(s) 895, 1065, 1099, 1209, 1335
BmtI GCTAGC 1 cut(s) 782
BplI GAGNNNNNCTC 2 cut(s) 431, 463
BpuEI CTTGAG 2 cut(s) 277, 433
Bsa29I ATCGAT 1 cut(s) 552
BsaBI GATNNNNATC 2 cut(s) 909, 1293
BsaJI CCNNGG 2 cut(s) 856, 1146
BsaXI ACNNNNNCTCC 2 cut(s) 324, 354
Bsc4I CCNNNNNNNGG 2 cut(s) 567, 845
Bse3DI GCAATG 2 cut(s) 816, 1174
Bse8I GATNNNNATC 2 cut(s) 909, 1293
BseBI CCWGG 2 cut(s) 490, 1320
BseCI ATCGAT 1 cut(s) 552
BseDI CCNNGG 2 cut(s) 856, 1146
BseGI GGATG 6 cut(s) 60, 838, 910, 1126, 1203, 1294
BseJI GATNNNNATC 2 cut(s) 909, 1293
BseLI CCNNNNNNNGG 2 cut(s) 567, 845
BseMI GCAATG 2 cut(s) 816, 1174
BseMII CTCAG 2 cut(s) 375, 621
BseRI GAGGAG 1 cut(s) 527
BseSI GKGCMC 1 cut(s) 855
BseXI GCAGC 2 cut(s) 1076, 1352
BsgI GTGCAG 1 cut(s) 285
BshFI GGCC 4 cut(s) 169, 838, 982, 1323
BshVI ATCGAT 1 cut(s) 552
BsiHKAI GWGCWC 3 cut(s) 296, 865, 1028
BslFI GGGAC 3 cut(s) 550, 675, 1127
BslI CCNNNNNNNGG 2 cut(s) 567, 845
BsmAI GTCTC 6 cut(s) 100, 155, 448, 605, 734, 1252
BsmBI CGTCTC 1 cut(s) 448
BsmFI GGGAC 3 cut(s) 550, 675, 1127
BsnI GGCC 4 cut(s) 169, 838, 982, 1323
Bsp1286I GDGCHC 5 cut(s) 52, 296, 855, 865, 1028
Bsp1407I TGTACA 1 cut(s) 871
Bsp143I GATC 6 cut(s) 4, 232, 328, 475, 1294, 1315
Bsp19I CCATGG 1 cut(s) 1146
BspACI CCGC 1 cut(s) 1324
BspANI GGCC 4 cut(s) 169, 838, 982, 1323
BspCNI CTCAG 2 cut(s) 376, 622
BspDI ATCGAT 1 cut(s) 552
BspHI TCATGA 1 cut(s) 436
BspLI GGNNCC 6 cut(s) 6, 234, 338, 817, 1143, 1317
BspOI GCTAGC 1 cut(s) 782
BspPI GGATC 6 cut(s) 12, 227, 240, 1302, 1310, 1323
BsrDI GCAATG 2 cut(s) 816, 1174
BsrGI TGTACA 1 cut(s) 871
BssECI CCNNGG 2 cut(s) 856, 1146
BssMI GATC 6 cut(s) 4, 232, 328, 475, 1294, 1315
BssT1I CCWWGG 2 cut(s) 856, 1146
Bst2UI CCWGG 2 cut(s) 490, 1320
Bst4CI ACNGT 4 cut(s) 175, 651, 820, 949
Bst6I CTCTTC 2 cut(s) 98, 1005
BstAUI TGTACA 1 cut(s) 871
BstC8I GCNNGC 3 cut(s) 776, 780, 957
BstDEI CTNAG 2 cut(s) 384, 630
BstDSI CCRYGG 1 cut(s) 1146
BstF5I GGATG 6 cut(s) 60, 838, 910, 1126, 1203, 1294
BstKTI GATC 6 cut(s) 7, 235, 331, 478, 1297, 1318
BstMAI GTCTC 6 cut(s) 100, 155, 448, 605, 734, 1252
BstMBI GATC 6 cut(s) 4, 232, 328, 475, 1294, 1315
BstNI CCWGG 2 cut(s) 490, 1320
BstNSI RCATGY 1 cut(s) 584
BstSCI CCNGG 2 cut(s) 488, 1318
BstSFI CTRYAG 1 cut(s) 1128
BstSLI GKGCMC 1 cut(s) 855
BstV1I GCAGC 2 cut(s) 1076, 1352
BstX2I RGATCY 3 cut(s) 4, 232, 1315
BstYI RGATCY 3 cut(s) 4, 232, 1315
Bsu15I ATCGAT 1 cut(s) 552
BsuRI GGCC 4 cut(s) 169, 838, 982, 1323
BsuTUI ATCGAT 1 cut(s) 552
BtgI CCRYGG 1 cut(s) 1146
BtsCI GGATG 6 cut(s) 60, 838, 910, 1126, 1203, 1294
BtsI GCAGTG 1 cut(s) 1140
BtsIMutI CAGTG 1 cut(s) 1140
Cac8I GCNNGC 3 cut(s) 776, 780, 957
CciI TCATGA 1 cut(s) 436
Cfr13I GGNCC 3 cut(s) 442, 836, 1141
ClaI ATCGAT 1 cut(s) 552
Csp6I GTAC 3 cut(s) 652, 872, 966
CspCI CAANNNNNGTGG 2 cut(s) 368, 403
CviQI GTAC 3 cut(s) 652, 872, 966
DdeI CTNAG 2 cut(s) 384, 630
DpnI GATC 6 cut(s) 6, 234, 330, 477, 1296, 1317
DpnII GATC 6 cut(s) 4, 232, 328, 475, 1294, 1315
EaeI YGGCCR 2 cut(s) 167, 1321
Eam1104I CTCTTC 2 cut(s) 98, 1005
EarI CTCTTC 2 cut(s) 98, 1005
Eco130I CCWWGG 2 cut(s) 856, 1146
Eco24I GRGCYC 1 cut(s) 52
Eco32I GATATC 1 cut(s) 550
Eco47I GGWCC 2 cut(s) 442, 1141
EcoRI GAATTC 1 cut(s) 181
EcoRII CCWGG 2 cut(s) 488, 1318
EcoRV GATATC 1 cut(s) 550
EcoT14I CCWWGG 2 cut(s) 856, 1146
EcoT22I ATGCAT 1 cut(s) 1309
EcoT38I GRGCYC 1 cut(s) 52
ErhI CCWWGG 2 cut(s) 856, 1146
Esp3I CGTCTC 1 cut(s) 448
FaqI GGGAC 3 cut(s) 550, 675, 1127
FauNDI CATATG 1 cut(s) 157
FbaI TGATCA 1 cut(s) 475
FblI GTMKAC 2 cut(s) 786, 1057
Fnu4HI GCNGC 3 cut(s) 1065, 1324, 1366
FokI GGATG 6 cut(s) 67, 845, 917, 1133, 1190, 1301
FriOI GRGCYC 1 cut(s) 52
Fsp4HI GCNGC 3 cut(s) 1065, 1324, 1366
FspBI CTAG 3 cut(s) 110, 779, 857
GluI GCNGC 3 cut(s) 1065, 1324, 1366
HaeIII GGCC 4 cut(s) 169, 838, 982, 1323
HindIII AAGCTT 1 cut(s) 393
HinfI GANTC 2 cut(s) 1124, 1349
HphI GGTGA 1 cut(s) 947
Hpy166II GTNNAC 2 cut(s) 787, 1058
Hpy188I TCNGA 3 cut(s) 22, 54, 216
Hpy188III TCNNGA 5 cut(s) 256, 289, 408, 419, 437
Hpy8I GTNNAC 2 cut(s) 787, 1058
Hpy99I CGWCG 1 cut(s) 336
HpyAV CCTTC 2 cut(s) 264, 1279
HpyCH4III ACNGT 4 cut(s) 175, 651, 820, 949
HpyCH4IV ACGT 1 cut(s) 625
HpyCH4V TGCA 9 cut(s) 266, 466, 641, 774, 809, 1090, 1222, 1242, 1307
HpyF3I CTNAG 2 cut(s) 384, 630
HpySE526I ACGT 1 cut(s) 625
Ksp22I TGATCA 1 cut(s) 475
Kzo9I GATC 6 cut(s) 4, 232, 328, 475, 1294, 1315
LmnI GCTCC 2 cut(s) 336, 860
Lsp1109I GCAGC 2 cut(s) 1076, 1352
LweI GCATC 5 cut(s) 895, 1065, 1099, 1209, 1335
MaeI CTAG 3 cut(s) 110, 779, 857
MaeII ACGT 1 cut(s) 625
MaeIII GTNAC 4 cut(s) 61, 596, 935, 1002
MalI GATC 6 cut(s) 6, 234, 330, 477, 1296, 1317
MboI GATC 6 cut(s) 4, 232, 328, 475, 1294, 1315
MfeI CAATTG 2 cut(s) 87, 882
MflI RGATCY 3 cut(s) 4, 232, 1315
MhlI GDGCHC 5 cut(s) 52, 296, 855, 865, 1028
MlsI TGGCCA 1 cut(s) 169
MluCI AATT 8 cut(s) 87, 150, 181, 422, 505, 636, 686, 882
MluNI TGGCCA 1 cut(s) 169
MmeI TCCRAC 3 cut(s) 32, 45, 810
Mox20I TGGCCA 1 cut(s) 169
Mph1103I ATGCAT 1 cut(s) 1309
MroXI GAANNNNTTC 2 cut(s) 221, 284
MscI TGGCCA 1 cut(s) 169
MseI TTAA 5 cut(s) 189, 299, 413, 998, 1372
MslI CAYNNNNRTG 2 cut(s) 471, 1302
Msp20I TGGCCA 1 cut(s) 169
MspR9I CCNGG 2 cut(s) 490, 1320
MunI CAATTG 2 cut(s) 87, 882
MvaI CCWGG 2 cut(s) 490, 1320
NcoI CCATGG 1 cut(s) 1146
NdeI CATATG 1 cut(s) 157
NdeII GATC 6 cut(s) 4, 232, 328, 475, 1294, 1315
NheI GCTAGC 1 cut(s) 778
NlaIV GGNNCC 6 cut(s) 6, 234, 338, 817, 1143, 1317
NmuCI GTSAC 2 cut(s) 935, 1002
NsiI ATGCAT 1 cut(s) 1309
NspI RCATGY 1 cut(s) 584
PagI TCATGA 1 cut(s) 436
PdmI GAANNNNTTC 2 cut(s) 221, 284
PfeI GAWTC 2 cut(s) 1124, 1349
PflMI CCANNNNNTGG 2 cut(s) 567, 845
PfoI TCCNGGA 1 cut(s) 488
PkrI GCNGC 3 cut(s) 1066, 1325, 1367
Psp6I CCWGG 2 cut(s) 488, 1318
PspGI CCWGG 2 cut(s) 488, 1318
PspN4I GGNNCC 6 cut(s) 6, 234, 338, 817, 1143, 1317
PspPI GGNCC 3 cut(s) 442, 836, 1141
PsuI RGATCY 3 cut(s) 4, 232, 1315
RsaI GTAC 3 cut(s) 653, 873, 967
RsaNI GTAC 3 cut(s) 652, 872, 966
RseI CAYNNNNRTG 2 cut(s) 471, 1302
SaqAI TTAA 5 cut(s) 189, 299, 413, 998, 1372
SatI GCNGC 3 cut(s) 1065, 1324, 1366
Sau3AI GATC 6 cut(s) 4, 232, 328, 475, 1294, 1315
Sau96I GGNCC 3 cut(s) 442, 836, 1141
ScrFI CCNGG 2 cut(s) 490, 1320
SduI GDGCHC 5 cut(s) 52, 296, 855, 865, 1028
SetI ASST 8 cut(s) 385, 397, 463, 628, 780, 937, 1266, 1342
SfaNI GCATC 5 cut(s) 895, 1065, 1099, 1209, 1335
SfcI CTRYAG 1 cut(s) 1128
SinI GGWCC 2 cut(s) 442, 1141
SmiMI CAYNNNNRTG 2 cut(s) 471, 1302
SmlI CTYRAG 2 cut(s) 256, 448
SmoI CTYRAG 2 cut(s) 256, 448
Sse9I AATT 8 cut(s) 87, 150, 181, 422, 505, 636, 686, 882
SsiI CCGC 1 cut(s) 1324
SspMI CTAG 3 cut(s) 110, 779, 857
StyD4I CCNGG 2 cut(s) 488, 1318
StyI CCWWGG 2 cut(s) 856, 1146
TaaI ACNGT 4 cut(s) 175, 651, 820, 949
TaiI ACGT 1 cut(s) 628
TaqI TCGA 5 cut(s) 331, 409, 481, 552, 985
TasI AATT 8 cut(s) 87, 150, 181, 422, 505, 636, 686, 882
TatI WGTACW 2 cut(s) 651, 871
TauI GCSGC 1 cut(s) 1326
TfiI GAWTC 2 cut(s) 1124, 1349
Tru1I TTAA 5 cut(s) 189, 299, 413, 998, 1372
Tru9I TTAA 5 cut(s) 189, 299, 413, 998, 1372
TscAI CASTG 1 cut(s) 1140
TseFI GTSAC 2 cut(s) 935, 1002
TseI GCWGC 2 cut(s) 1064, 1365
Tsp45I GTSAC 2 cut(s) 935, 1002
TspDTI ATGAA 8 cut(s) 20, 214, 361, 1023, 1137, 1174, 1298, 1350
TspGWI ACGGA 1 cut(s) 349
TspRI CASTG 1 cut(s) 1140
Van91I CCANNNNNTGG 2 cut(s) 567, 845
VpaK11BI GGWCC 2 cut(s) 442, 1141
XapI RAATTY 2 cut(s) 181, 422
XceI RCATGY 1 cut(s) 584
XmaJI CCTAGG 1 cut(s) 856
XmiI GTMKAC 2 cut(s) 786, 1057
XmnI GAANNNNTTC 2 cut(s) 221, 284
XspI CTAG 3 cut(s) 110, 779, 857
Zsp2I ATGCAT 1 cut(s) 1309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.