Rh2DG224300

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000321
Physical Location & Seq
Forward (+)
21770456 .. 21772904
2449 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG224300.1

Sequence Viewer

Length: 1539 bp
ATGGATTCAAAAGAAGAAAATCATGCCACTGAAGATGACTTGCCATCTGACGAGAAGAAGGGAAGAGGTCCAACTCTCATGTCCGATATTATTCACGGTAGGAGTAAGGGGGCTCGAATGGAAGTGACATATAACAAAAAGGGGCAACCAATTGGTCTTGGAGGGAAGAGGCTAGCTACTTTTATTGGGGTACTGGCTCGAACTACTATCCCAATCACATATGAGACTTGGCCAGCCGTGAAGAATTCACTTAAAGAAATGATATGGAGTATGGTTCAGAAATCATTCATTGTGGATCCAAGAAGCAAGAAAGATGTCTTGAGTAGTGCAGGAAGGAAATGGAAATCATTCAAGAGCACTTTAACTACAAAATATATATTGAAGTATAAAGATCAGCGCAGCCTCCTAAAGAAAAAACCTGAAGAATATGAGTTTATTACTCAACCTGAGTGGGAAGCTTTTGTGAAGTCTCGATTAACTCCAGAATTTTTGGAAATTCATGAGGACCACTCAAGGAGACGAGCTTTGAATGAGTATGATCATCGAATGTCTAGGAAAGGCTATGCTAATTTGGAAGAGGAACTAAAAATGGAGTTAGGGACTGAAGACGATATTGATAGAGCCATCTTATGGAAGAAAGGGCGTGTCGATAAAGAGGGTAACTATTTGAGTGAGACAACCAAACAACGTGCTGACAAAATTGATGCTTTAACGAAAGATGTGAGGGAGGGAATTGTGTCTGCTGTTGGTAGGAACGACATTTTGACTCAAGCTTTGGAGACACCTGAGCAATCGGGCCGTGTAAGAGGTGCTAGACAATTTGTTACACATAAGGTGTACTTTAATACATCTAGATATAAGTCTGCAACCAAGACACAATTGTTGGAACAGCAGTTGGAATTGATGCAAAACCAGATCAACATGTTTGCTTCATTATTGGATCCCGAGAAGTTGGATCTAGTTAAACTAGGCATGATTCGAGACATGTTCAGATGTAATAATGGATCTGAAAAAGCTAGTTGCTCAGTCGACAAGGAGAAAAATCTGTCTTCTAAGGAGGAGGTATCTAAGGTGGCATCTGAGAAAGAAAAAGAGAAAAAAGCTACTGCCAAAAAGGATCGAAAATGGGATGATACTCCATCTCCAATTGACAGCAGTAGAGAAAGCAAGAAGGCAAAACAATTGTATAAAGGGGGACTAATGATGAGACAGAGAGGAGCATTGGAAGATGACAATGTGATAACATCAACAGATAAAGCATATATTCCTATTGAGACAACAAATGTTGTAAAAAAGAACTCAATGAGCACTTGGAAGTCTTCGAGTAAAACACCACGCAAACAAGCAGCATACAAGATGCCTGTGTCCGTGCATCTTCTACTACGTTTGGCAAGAACAATGGATGAATCTATAGCAGTGTCGGTCCCAATGGAAGAAGGTGTGTTTGGCAATGACCACAATACATTCATAAACGGTAACGACATCATCCAGTTTGTTCGATGCAGCCAATATCCACTATTTGCATTTCTATCTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 Transposase_24

Protein Analysis

512

Amino Acids

58.55

Weight (kDa)

9.38

Isoelectric Point (pI)

37.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transposase_24 PF03004 151 - 277 1.6e-09 Plant transposase (Ptta/En/Spm family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000321)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221
prunus_persica Prupe.6G157700_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1
pyrus_communis pycom03g09490 pycom03g19580 pycom07g21940 pycom07g27440 pycom14g09710
rosa_chinensis RchiOBHm_Chr1g0318231 RchiOBHm_Chr1g0318241 RchiOBHm_Chr1g0325001 RchiOBHm_Chr1g0325021 RchiOBHm_Chr2g0112301 RchiOBHm_Chr3g0482281 RchiOBHm_Chr3g0482291 RchiOBHm_Chr4g0390561 RchiOBHm_Chr4g0390571 RchiOBHm_Chr4g0417951 RchiOBHm_Chr7g0232611 RchiOBHm_Chr7g0240601
rosa_laevigata RLG00000001069 RLG00000013674 RLG00000018830 RLG00000020747
rosa_multiflora Rmu_co8364947.1_g000001 Rmu_co8416715.1_g000001 Rmu_sc0001348.1_g000014 Rmu_sc0002986.1_g000026 Rmu_sc0004003.1_g000007 Rmu_sc0005137.1_g000014 Rmu_sc0006583.1_g000016
rosa_roxburghii Rroxscaffold_159G00432800 Rroxscaffold_174G00435170 Rroxscaffold_1G00001870 Rroxscaffold_1G00044480 Rroxscaffold_2G00111880 Rroxscaffold_2G00111890 Rroxscaffold_2G00119670 Rroxscaffold_2G00129450 Rroxscaffold_2G00131520 Rroxscaffold_3G00240880 Rroxscaffold_5G00337440 Rroxscaffold_5G00356220 Rroxscaffold_5G00362390 Rroxscaffold_5G00368260 Rroxscaffold_7G00196230 Rroxscaffold_7G00196240 Rroxscaffold_7G00202020
rosa_rugosa Rorug01G0094700 Rorug02G0050500 Rorug02G0179800 Rorug05G0042800 Rorug05G0043000 Rorug05G0043100 Rorug05G0043200 Rorug06G0045300 Rorug06G0045600 Rorug06G0069400 Rorug07G0213000 Rorug07G0213000
rosa_samantha Rh1AG031600 Rh1DG131700 Rh1DG134800 Rh1DG288000 Rh1DG288100 Rh2AG218500 Rh2AG232500 Rh2BG245900 Rh2CG236700 Rh2DG224300 Rh2DG224400 Rh2DG240200 Rh3AG247300 Rh3BG126600 Rh3BG126700 Rh3BG333600 Rh5AG440500 Rh5AG440600 Rh5BG457800 Rh5DG472700 Rh5DG472800 Rh6BG138000 Rh6BG138100 Rh6BG171500 Rh6BG188400 Rh6BG230000 Rh6CG135600 Rh6CG135700 Rh6CG186300 Rh6CG450300 Rh7AG376100 Rh7CG394800 Rh7CG426100 Rh7CG426200 Rh7DG247700 Rh7DG322600 Rh7DG334300 Rh7DG364700
rosa_wichuraiana Rw2G018040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 630
AccI GTMKAC 1 cut(s) 1029
AclWI GGATC 7 cut(s) 290, 303, 935, 948, 963, 1012, 1125
AcoI YGGCCR 1 cut(s) 230
AcsI RAATTY 3 cut(s) 244, 485, 495
AcuI CTGAAG 3 cut(s) 51, 441, 624
AfaI GTAC 2 cut(s) 192, 839
AfiI CCNNNNNNNGG 1 cut(s) 630
AflIII ACRYGT 2 cut(s) 921, 984
AgsI TTSAA 4 cut(s) 9, 352, 382, 529
AjuI GAANNNNNNNTTGG 2 cut(s) 1428, 1460
AluBI AGCT 6 cut(s) 176, 458, 524, 773, 1016, 1103
AluI AGCT 6 cut(s) 176, 458, 524, 773, 1016, 1103
Alw21I GWGCWC 2 cut(s) 359, 1310
Alw26I GTCTC 8 cut(s) 218, 474, 511, 668, 773, 975, 1201, 1268
AlwI GGATC 7 cut(s) 290, 303, 935, 948, 963, 1012, 1125
Ama87I CYCGRG 1 cut(s) 944
AoxI GGCC 2 cut(s) 230, 796
ApeKI GCWGC 3 cut(s) 399, 1346, 1503
ApoI RAATTY 3 cut(s) 244, 485, 495
ArsI GACNNNNNNTTYG 2 cut(s) 757, 789
Asp700I GAANNNNTTC 2 cut(s) 284, 347
AspLEI GCGC 1 cut(s) 399
AspS9I GGNCC 4 cut(s) 68, 505, 796, 1423
AsuNHI GCTAGC 1 cut(s) 172
AvaI CYCGRG 1 cut(s) 944
AvaII GGWCC 3 cut(s) 68, 505, 1423
BalI TGGCCA 1 cut(s) 232
BamHI GGATCC 2 cut(s) 295, 940
BanII GRGCYC 1 cut(s) 115
BbsI GAAGAC 3 cut(s) 612, 1041, 1311
Bbv12I GWGCWC 2 cut(s) 359, 1310
BbvI GCAGC 3 cut(s) 411, 1358, 1515
BccI CCATC 3 cut(s) 52, 632, 1147
BceAI ACGGC 2 cut(s) 221, 783
BclI TGATCA 1 cut(s) 538
BcoDI GTCTC 8 cut(s) 218, 474, 511, 668, 773, 975, 1201, 1268
BfaI CTAG 7 cut(s) 173, 552, 813, 852, 959, 968, 1017
BfmI CTRYAG 1 cut(s) 1410
BisI GCNGC 3 cut(s) 400, 1347, 1504
BlsI GCNGC 3 cut(s) 401, 1348, 1505
Bme18I GGWCC 3 cut(s) 68, 505, 1423
BmeT110I CYCGRG 1 cut(s) 944
BmgT120I GGNCC 4 cut(s) 68, 505, 796, 1423
BmiI GGNNCC 3 cut(s) 297, 942, 1425
BmsI GCATC 6 cut(s) 694, 894, 1085, 1347, 1381, 1490
BmtI GCTAGC 1 cut(s) 176
BpiI GAAGAC 3 cut(s) 612, 1041, 1311
BplI GAGNNNNNCTC 2 cut(s) 494, 526
BpmI CTGGAG 1 cut(s) 465
Bpu10I CCTNAGC 1 cut(s) 786
BpuEI CTTGAG 3 cut(s) 340, 496, 753
BsaXI ACNNNNNCTCC 2 cut(s) 1126, 1156
Bsc4I CCNNNNNNNGG 1 cut(s) 630
Bse1I ACTGG 2 cut(s) 198, 1489
Bse3DI GCAATG 1 cut(s) 1456
BseGI GGATG 3 cut(s) 1135, 1408, 1485
BseLI CCNNNNNNNGG 1 cut(s) 630
BseMI GCAATG 1 cut(s) 1456
BseMII CTCAG 4 cut(s) 438, 777, 1038, 1071
BseNI ACTGG 2 cut(s) 198, 1489
BseRI GAGGAG 2 cut(s) 1073, 1230
BseXI GCAGC 3 cut(s) 411, 1358, 1515
BsgI GTGCAG 1 cut(s) 348
BshFI GGCC 2 cut(s) 232, 798
BsiHKAI GWGCWC 2 cut(s) 359, 1310
BsiHKCI CYCGRG 1 cut(s) 944
BslFI GGGAC 3 cut(s) 613, 1209, 1409
BslI CCNNNNNNNGG 1 cut(s) 630
BsmAI GTCTC 8 cut(s) 218, 474, 511, 668, 773, 975, 1201, 1268
BsmBI CGTCTC 1 cut(s) 511
BsmFI GGGAC 3 cut(s) 613, 1209, 1409
BsnI GGCC 2 cut(s) 232, 798
BsoBI CYCGRG 1 cut(s) 944
Bsp1286I GDGCHC 3 cut(s) 115, 359, 1310
Bsp143I GATC 8 cut(s) 295, 391, 538, 915, 940, 955, 1004, 1117
BspANI GGCC 2 cut(s) 232, 798
BspCNI CTCAG 4 cut(s) 439, 778, 1037, 1072
BspHI TCATGA 1 cut(s) 499
BspLI GGNNCC 3 cut(s) 297, 942, 1425
BspOI GCTAGC 1 cut(s) 176
BspPI GGATC 7 cut(s) 290, 303, 935, 948, 963, 1012, 1125
BsrDI GCAATG 1 cut(s) 1456
BsrI ACTGG 2 cut(s) 198, 1489
BssMI GATC 8 cut(s) 295, 391, 538, 915, 940, 955, 1004, 1117
Bst4CI ACNGT 2 cut(s) 98, 1475
Bst6I CTCTTC 3 cut(s) 58, 161, 570
BstC8I GCNNGC 2 cut(s) 174, 234
BstDEI CTNAG 6 cut(s) 447, 786, 1024, 1053, 1068, 1080
BstF5I GGATG 3 cut(s) 1135, 1408, 1485
BstHHI GCGC 1 cut(s) 399
BstKTI GATC 8 cut(s) 298, 394, 541, 918, 943, 958, 1007, 1120
BstMAI GTCTC 8 cut(s) 218, 474, 511, 668, 773, 975, 1201, 1268
BstMBI GATC 8 cut(s) 295, 391, 538, 915, 940, 955, 1004, 1117
BstNSI RCATGY 2 cut(s) 925, 988
BstSFI CTRYAG 1 cut(s) 1410
BstV1I GCAGC 3 cut(s) 411, 1358, 1515
BstV2I GAAGAC 3 cut(s) 612, 1041, 1311
BstX2I RGATCY 4 cut(s) 295, 940, 955, 1004
BstYI RGATCY 4 cut(s) 295, 940, 955, 1004
BsuRI GGCC 2 cut(s) 232, 798
BtsCI GGATG 3 cut(s) 1135, 1408, 1485
BtsI GCAGTG 1 cut(s) 1422
BtsIMutI CAGTG 2 cut(s) 27, 1422
Cac8I GCNNGC 2 cut(s) 174, 234
CciI TCATGA 1 cut(s) 499
CfoI GCGC 1 cut(s) 399
Cfr13I GGNCC 4 cut(s) 68, 505, 796, 1423
Csp6I GTAC 2 cut(s) 191, 838
CspCI CAANNNNNGTGG 2 cut(s) 431, 466
CviAII CATG 7 cut(s) 23, 79, 500, 922, 973, 985, 1536
CviQI GTAC 2 cut(s) 191, 838
DdeI CTNAG 6 cut(s) 447, 786, 1024, 1053, 1068, 1080
DpnI GATC 8 cut(s) 297, 393, 540, 917, 942, 957, 1006, 1119
DpnII GATC 8 cut(s) 295, 391, 538, 915, 940, 955, 1004, 1117
EaeI YGGCCR 1 cut(s) 230
Eam1104I CTCTTC 3 cut(s) 58, 161, 570
EarI CTCTTC 3 cut(s) 58, 161, 570
Eco24I GRGCYC 1 cut(s) 115
Eco47I GGWCC 3 cut(s) 68, 505, 1423
Eco57I CTGAAG 3 cut(s) 51, 441, 624
Eco88I CYCGRG 1 cut(s) 944
EcoRI GAATTC 1 cut(s) 244
EcoT38I GRGCYC 1 cut(s) 115
Esp3I CGTCTC 1 cut(s) 511
FaeI CATG 7 cut(s) 26, 82, 503, 925, 976, 988, 1539
FalI AAGNNNNNCTT 2 cut(s) 824, 856
FaqI GGGAC 3 cut(s) 613, 1209, 1409
FatI CATG 7 cut(s) 22, 78, 499, 921, 972, 984, 1535
FauNDI CATATG 1 cut(s) 220
FbaI TGATCA 1 cut(s) 538
FblI GTMKAC 1 cut(s) 1029
Fnu4HI GCNGC 3 cut(s) 400, 1347, 1504
FokI GGATG 3 cut(s) 1142, 1415, 1472
FriOI GRGCYC 1 cut(s) 115
Fsp4HI GCNGC 3 cut(s) 400, 1347, 1504
FspBI CTAG 7 cut(s) 173, 552, 813, 852, 959, 968, 1017
GlaI GCGC 1 cut(s) 398
GluI GCNGC 3 cut(s) 400, 1347, 1504
GsuI CTGGAG 1 cut(s) 465
HaeIII GGCC 2 cut(s) 232, 798
HhaI GCGC 1 cut(s) 399
Hin1II CATG 7 cut(s) 26, 82, 503, 925, 976, 988, 1539
Hin6I GCGC 1 cut(s) 397
HinP1I GCGC 1 cut(s) 397
HincII GTYRAC 1 cut(s) 1030
HindII GTYRAC 1 cut(s) 1030
HindIII AAGCTT 2 cut(s) 456, 771
HinfI GANTC 4 cut(s) 5, 766, 976, 1406
Hpy166II GTNNAC 2 cut(s) 838, 1030
Hpy188I TCNGA 6 cut(s) 49, 85, 279, 992, 1009, 1081
Hpy188III TCNNGA 8 cut(s) 319, 352, 471, 482, 500, 852, 944, 980
Hpy8I GTNNAC 2 cut(s) 838, 1030
HpyAV CCTTC 4 cut(s) 52, 327, 1165, 1430
HpyCH4III ACNGT 2 cut(s) 98, 1475
HpyCH4IV ACGT 2 cut(s) 688, 1384
HpyCH4V TGCA 6 cut(s) 329, 866, 907, 1372, 1503, 1523
HpyF3I CTNAG 6 cut(s) 447, 786, 1024, 1053, 1068, 1080
HpySE526I ACGT 2 cut(s) 688, 1384
Hsp92II CATG 7 cut(s) 26, 82, 503, 925, 976, 988, 1539
HspAI GCGC 1 cut(s) 397
Ksp22I TGATCA 1 cut(s) 538
Kzo9I GATC 8 cut(s) 295, 391, 538, 915, 940, 955, 1004, 1117
LmnI GCTCC 1 cut(s) 1217
Lsp1109I GCAGC 3 cut(s) 411, 1358, 1515
LweI GCATC 6 cut(s) 694, 894, 1085, 1347, 1381, 1490
MaeI CTAG 7 cut(s) 173, 552, 813, 852, 959, 968, 1017
MaeII ACGT 2 cut(s) 688, 1384
MaeIII GTNAC 4 cut(s) 124, 659, 823, 1475
MalI GATC 8 cut(s) 297, 393, 540, 917, 942, 957, 1006, 1119
MboI GATC 8 cut(s) 295, 391, 538, 915, 940, 955, 1004, 1117
MfeI CAATTG 4 cut(s) 150, 878, 1146, 1181
MflI RGATCY 4 cut(s) 295, 940, 955, 1004
MhlI GDGCHC 3 cut(s) 115, 359, 1310
MlsI TGGCCA 1 cut(s) 232
MluNI TGGCCA 1 cut(s) 232
MlyI GAGTC 1 cut(s) 760
MmeI TCCRAC 4 cut(s) 95, 864, 876, 933
Mox20I TGGCCA 1 cut(s) 232
MroXI GAANNNNTTC 2 cut(s) 284, 347
MscI TGGCCA 1 cut(s) 232
MseI TTAA 6 cut(s) 252, 362, 476, 710, 843, 963
Msp20I TGGCCA 1 cut(s) 232
MunI CAATTG 4 cut(s) 150, 878, 1146, 1181
NdeI CATATG 1 cut(s) 220
NdeII GATC 8 cut(s) 295, 391, 538, 915, 940, 955, 1004, 1117
NheI GCTAGC 1 cut(s) 172
NlaIII CATG 7 cut(s) 26, 82, 503, 925, 976, 988, 1539
NlaIV GGNNCC 3 cut(s) 297, 942, 1425
NmuCI GTSAC 1 cut(s) 124
NspI RCATGY 2 cut(s) 925, 988
PagI TCATGA 1 cut(s) 499
PciI ACATGT 2 cut(s) 921, 984
PdmI GAANNNNTTC 2 cut(s) 284, 347
PfeI GAWTC 3 cut(s) 5, 976, 1406
PflMI CCANNNNNTGG 1 cut(s) 630
PkrI GCNGC 3 cut(s) 401, 1348, 1505
PleI GAGTC 1 cut(s) 760
PpsI GAGTC 1 cut(s) 760
PscI ACATGT 2 cut(s) 921, 984
PspN4I GGNNCC 3 cut(s) 297, 942, 1425
PspPI GGNCC 4 cut(s) 68, 505, 796, 1423
PsuI RGATCY 4 cut(s) 295, 940, 955, 1004
RsaI GTAC 2 cut(s) 192, 839
RsaNI GTAC 2 cut(s) 191, 838
SalI GTCGAC 1 cut(s) 1028
SaqAI TTAA 6 cut(s) 252, 362, 476, 710, 843, 963
SatI GCNGC 3 cut(s) 400, 1347, 1504
Sau3AI GATC 8 cut(s) 295, 391, 538, 915, 940, 955, 1004, 1117
Sau96I GGNCC 4 cut(s) 68, 505, 796, 1423
SchI GAGTC 1 cut(s) 760
SduI GDGCHC 3 cut(s) 115, 359, 1310
SfaNI GCATC 6 cut(s) 694, 894, 1085, 1347, 1381, 1490
SfcI CTRYAG 1 cut(s) 1410
SinI GGWCC 3 cut(s) 68, 505, 1423
SmlI CTYRAG 3 cut(s) 319, 511, 768
SmoI CTYRAG 3 cut(s) 319, 511, 768
SspMI CTAG 7 cut(s) 173, 552, 813, 852, 959, 968, 1017
TaaI ACNGT 2 cut(s) 98, 1475
TaiI ACGT 2 cut(s) 691, 1387
TaqII GACCGA 1 cut(s) 1411
TatI WGTACW 1 cut(s) 837
TfiI GAWTC 3 cut(s) 5, 976, 1406
Tru1I TTAA 6 cut(s) 252, 362, 476, 710, 843, 963
Tru9I TTAA 6 cut(s) 252, 362, 476, 710, 843, 963
TscAI CASTG 2 cut(s) 34, 1422
TseFI GTSAC 1 cut(s) 124
TseI GCWGC 3 cut(s) 399, 1346, 1503
Tsp45I GTSAC 1 cut(s) 124
TspDTI ATGAA 5 cut(s) 277, 488, 921, 1419, 1456
TspGWI ACGGA 1 cut(s) 1357
TspRI CASTG 2 cut(s) 34, 1422
Van91I CCANNNNNTGG 1 cut(s) 630
VpaK11BI GGWCC 3 cut(s) 68, 505, 1423
XapI RAATTY 3 cut(s) 244, 485, 495
XbaI TCTAGA 1 cut(s) 851
XceI RCATGY 2 cut(s) 925, 988
XmiI GTMKAC 1 cut(s) 1029
XmnI GAANNNNTTC 2 cut(s) 284, 347
XspI CTAG 7 cut(s) 173, 552, 813, 852, 959, 968, 1017
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.