Rorug06G0045300

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000321
Physical Location & Seq
Forward (+)
5969833 .. 5972582
2750 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0045300.1

Sequence Viewer

Length: 1443 bp
ATGAGAGTTCACCATCCCACTCACCTCCGATCCAAGTCCGGCATTGACGACGACCCGGACCCGACCCGGACCATAATGTCGGGGCCGGGCATGACCGTCGGCGGCCCGCTACGGAAGAAAGGCATGGGAGTACGGCCATGGCTGCTCCTCGACTCGAACGGACAAGCTCAGGTGGTGGAGGTCGGAAAGCACGCCATCATGCGCCGGACGGGTCTACCCGCCCGCGACCTCCGGATCCTCGACCCGCTGCTCTCGTACCCGTCGACGGTTCTTGGTCGGGAGAGAGCGATCGTCATTAACCTGGAGCACATCAAGGCCATCATTACTGCGCAGGAGGTTCTCTTGTTGAATTCAAGAGACCCGTCGGTGATTCCCTTCATCGATGAGCTTCAGCGCCGGCTCTCTCGCCATAGCCAAGCCACCAAGGCCATGAAAGCCCAGGAGGGGAATGGCGAAGAATCGAAATGGTGCGACTTGGAAGAGGCAGAGGCACATTTGAGAGGTGTTGGTGCTGGGGGAACTCAGGGAGGTGAAGGGACTGGGAAGCAAGGTCAGGAGGAGAATCGCGAAGGATTGAAGGTACTTCCTTTCGAGTTTGTGGCATTGGAGACATGCCTCGAAGCTGCTTGCAGTTGCTTAGAGAATGAAGCAAGGACATTGGAGCTAGAAGCTCACCCGGCTTTGGATAAGCTCACGTCGAAGATTAGTACACTCAATTTGGAACGCGTCCGTCAAATCAAGAGTCGCTTGGTGGCTATTACCGGTCGTGTTCAGAAGGTGAGGGATGAACTAGAACACTTGCTAGATGATGATGACGATATGGCTGAAATGTATTTAACTGAAAAGTTACAACTTGAATATTCTACTACCTCATCTTTGACTGAGAGAGATGATATGAATGATGAAGCTCCACAAACAGATATAGATGACAGGAATCTTGCTGAAATATCACTGGATGCTACTGGGGAATCCTTGAATTTTGAAGGCGATCATCGAAACACTGATAACCCCCCGCATGAATCCCTTGACACCCATGGCACTCATACTAGCAGCACTCATAGTGTTACAGGAAAGACCCTTGATGTGGAGGAGCTTGAAATGCTATTGGAGGCATATTTTGTGCAAATTGATGGTACACTAAACAAGTTATCCACGCTGAGGGAGTATGTCGATGACACAGAGGATTACATCAACATAATGTTGGATGACAAACAGAACCATCTCCTGCAAATGGGGGTTATGTTGACAACAGCAACCCTGGTAGTGAGTGCCTTTGTTGTTGTGGCTGGTATTTTTGGCATGAATATCAAAATTGAGCTGTTTGATCCCAATAAAGCAGGAATGCAAGATTGGCTCTGGACTATTGGTGGCAGCACTATAGGCTCCGTAGTCTTGTATATAATTGCCATTGGTTGGTGCAAGCATAAACGCCTGCTGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 Transposase_24

Protein Analysis

480

Amino Acids

53.33

Weight (kDa)

4.99

Isoelectric Point (pI)

36.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRS2-like PF22099 50 - 140 5.1e-11 Magnesium transporter MRS2-like
MRS2-like PF22099 193 - 284 2e-15 Magnesium transporter MRS2-like
MRS2-like PF22099 360 - 403 2.5e-07 Magnesium transporter MRS2-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000321)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221
prunus_persica Prupe.6G157700_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1
pyrus_communis pycom03g09490 pycom03g19580 pycom07g21940 pycom07g27440 pycom14g09710
rosa_chinensis RchiOBHm_Chr1g0318231 RchiOBHm_Chr1g0318241 RchiOBHm_Chr1g0325001 RchiOBHm_Chr1g0325021 RchiOBHm_Chr2g0112301 RchiOBHm_Chr3g0482281 RchiOBHm_Chr3g0482291 RchiOBHm_Chr4g0390561 RchiOBHm_Chr4g0390571 RchiOBHm_Chr4g0417951 RchiOBHm_Chr7g0232611 RchiOBHm_Chr7g0240601
rosa_laevigata RLG00000001069 RLG00000013674 RLG00000018830 RLG00000020747
rosa_multiflora Rmu_co8364947.1_g000001 Rmu_co8416715.1_g000001 Rmu_sc0001348.1_g000014 Rmu_sc0002986.1_g000026 Rmu_sc0004003.1_g000007 Rmu_sc0005137.1_g000014 Rmu_sc0006583.1_g000016
rosa_roxburghii Rroxscaffold_159G00432800 Rroxscaffold_174G00435170 Rroxscaffold_1G00001870 Rroxscaffold_1G00044480 Rroxscaffold_2G00111880 Rroxscaffold_2G00111890 Rroxscaffold_2G00119670 Rroxscaffold_2G00129450 Rroxscaffold_2G00131520 Rroxscaffold_3G00240880 Rroxscaffold_5G00337440 Rroxscaffold_5G00356220 Rroxscaffold_5G00362390 Rroxscaffold_5G00368260 Rroxscaffold_7G00196230 Rroxscaffold_7G00196240 Rroxscaffold_7G00202020
rosa_rugosa Rorug01G0094700 Rorug02G0050500 Rorug02G0179800 Rorug05G0042800 Rorug05G0043000 Rorug05G0043100 Rorug05G0043200 Rorug06G0045300 Rorug06G0045600 Rorug06G0069400 Rorug07G0213000 Rorug07G0213000
rosa_samantha Rh1AG031600 Rh1DG131700 Rh1DG134800 Rh1DG288000 Rh1DG288100 Rh2AG218500 Rh2AG232500 Rh2BG245900 Rh2CG236700 Rh2DG224300 Rh2DG224400 Rh2DG240200 Rh3AG247300 Rh3BG126600 Rh3BG126700 Rh3BG333600 Rh5AG440500 Rh5AG440600 Rh5BG457800 Rh5DG472700 Rh5DG472800 Rh6BG138000 Rh6BG138100 Rh6BG171500 Rh6BG188400 Rh6BG230000 Rh6CG135600 Rh6CG135700 Rh6CG186300 Rh6CG450300 Rh7AG376100 Rh7CG394800 Rh7CG426100 Rh7CG426200 Rh7DG247700 Rh7DG322600 Rh7DG334300 Rh7DG364700
rosa_wichuraiana Rw2G018040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 76
Acc16I TGCGCA 1 cut(s) 330
AccB7I CCANNNNNTGG 1 cut(s) 1413
AccI GTMKAC 2 cut(s) 214, 263
AccII CGCG 3 cut(s) 225, 567, 726
AccIII TCCGGA 1 cut(s) 231
AciI CCGC 6 cut(s) 102, 107, 219, 223, 245, 1013
AclWI GGATC 4 cut(s) 24, 229, 242, 1319
AcoI YGGCCR 1 cut(s) 134
AcsI RAATTY 2 cut(s) 349, 976
AcuI CTGAAG 1 cut(s) 374
AfaI GTAC 5 cut(s) 132, 257, 582, 709, 1135
AfiI CCNNNNNNNGG 7 cut(s) 265, 444, 682, 1084, 1158, 1231, 1413
AflIII ACRYGT 1 cut(s) 724
AgeI ACCGGT 1 cut(s) 761
AgsI TTSAA 7 cut(s) 349, 354, 577, 857, 976, 983, 1097
AjiI CACGTC 1 cut(s) 696
AjnI CCWGG 3 cut(s) 300, 438, 1257
AjuI GAANNNNNNNTTGG 2 cut(s) 1333, 1365
AluBI AGCT 9 cut(s) 167, 388, 623, 664, 671, 691, 908, 1093, 1318
AluI AGCT 9 cut(s) 167, 388, 623, 664, 671, 691, 908, 1093, 1318
Alw21I GWGCWC 1 cut(s) 309
Alw26I GTCTC 2 cut(s) 351, 602
AlwI GGATC 4 cut(s) 24, 229, 242, 1319
Aor13HI TCCGGA 1 cut(s) 231
AoxI GGCC 5 cut(s) 83, 103, 134, 315, 426
ApeKI GCWGC 5 cut(s) 142, 247, 623, 1050, 1371
ApoI RAATTY 2 cut(s) 349, 976
ArsI GACNNNNNNTTYG 2 cut(s) 727, 759
AsiGI ACCGGT 1 cut(s) 761
AspLEI GCGC 3 cut(s) 204, 331, 396
AspS9I GGNCC 4 cut(s) 58, 69, 83, 104
AsuC2I CCSGG 4 cut(s) 56, 67, 87, 677
AsuHPI GGTGA 5 cut(s) 14, 379, 542, 665, 790
AvaII GGWCC 2 cut(s) 58, 69
BamHI GGATCC 1 cut(s) 234
Bbv12I GWGCWC 1 cut(s) 309
BbvCI CCTCAGC 1 cut(s) 1157
BbvI GCAGC 5 cut(s) 129, 234, 610, 1062, 1383
BccI CCATC 5 cut(s) 21, 203, 326, 1124, 1227
BceAI ACGGC 1 cut(s) 149
BcgI CGANNNNNNTGC 2 cut(s) 79, 113
BciT130I CCWGG 3 cut(s) 302, 440, 1259
BcnI CCSGG 4 cut(s) 56, 67, 87, 677
BcoDI GTCTC 2 cut(s) 351, 602
BfaI CTAG 4 cut(s) 665, 791, 803, 1047
BfmI CTRYAG 1 cut(s) 1377
BfoI RGCGCY 1 cut(s) 397
BglI GCCNNNNNGGC 1 cut(s) 425
BisI GCNGC 6 cut(s) 103, 143, 248, 624, 1051, 1372
BlsI GCNGC 6 cut(s) 104, 144, 249, 625, 1052, 1373
Bme1390I CCNGG 7 cut(s) 56, 67, 87, 302, 440, 677, 1259
Bme18I GGWCC 2 cut(s) 58, 69
BmgBI CACGTC 1 cut(s) 696
BmgT120I GGNCC 4 cut(s) 58, 69, 83, 104
BmiI GGNNCC 4 cut(s) 60, 84, 236, 1384
BmrFI CCNGG 7 cut(s) 56, 67, 87, 302, 440, 677, 1259
BmrI ACTGGG 2 cut(s) 549, 972
BmsI GCATC 1 cut(s) 946
BmuI ACTGGG 2 cut(s) 549, 972
BpmI CTGGAG 1 cut(s) 323
Bpu10I CCTNAGC 2 cut(s) 168, 1157
BpuMI CCSGG 4 cut(s) 56, 67, 87, 677
Bsa29I ATCGAT 1 cut(s) 381
BsaI GGTCTC 1 cut(s) 351
BsaJI CCNNGG 5 cut(s) 137, 423, 438, 1033, 1257
BsaWI WCCGGW 2 cut(s) 231, 761
Bsc4I CCNNNNNNNGG 7 cut(s) 265, 444, 682, 1084, 1158, 1231, 1413
Bse118I RCCGGY 2 cut(s) 396, 761
Bse1I ACTGG 3 cut(s) 544, 957, 967
BseAI TCCGGA 1 cut(s) 231
BseBI CCWGG 3 cut(s) 302, 440, 1259
BseCI ATCGAT 1 cut(s) 381
BseDI CCNNGG 5 cut(s) 137, 423, 438, 1033, 1257
BseGI GGATG 4 cut(s) 13, 790, 961, 1210
BseLI CCNNNNNNNGG 7 cut(s) 265, 444, 682, 1084, 1158, 1231, 1413
BseMII CTCAG 4 cut(s) 182, 536, 873, 1148
BseNI ACTGG 3 cut(s) 544, 957, 967
BseRI GAGGAG 3 cut(s) 137, 572, 1103
BseXI GCAGC 5 cut(s) 129, 234, 610, 1062, 1383
BseYI CCCAGC 1 cut(s) 512
Bsh1236I CGCG 3 cut(s) 225, 567, 726
Bsh1285I CGRYCG 2 cut(s) 291, 766
BshFI GGCC 5 cut(s) 85, 105, 136, 317, 428
BshTI ACCGGT 1 cut(s) 761
BshVI ATCGAT 1 cut(s) 381
BsiEI CGRYCG 2 cut(s) 291, 766
BsiHKAI GWGCWC 1 cut(s) 309
BsiSI CCGG 9 cut(s) 39, 56, 67, 86, 205, 232, 397, 677, 762
BslFI GGGAC 1 cut(s) 550
BslI CCNNNNNNNGG 7 cut(s) 265, 444, 682, 1084, 1158, 1231, 1413
BsmAI GTCTC 2 cut(s) 351, 602
BsmFI GGGAC 1 cut(s) 550
BsmI GAATGC 1 cut(s) 1347
BsnI GGCC 5 cut(s) 85, 105, 136, 317, 428
Bso31I GGTCTC 1 cut(s) 351
Bsp1286I GDGCHC 1 cut(s) 309
Bsp13I TCCGGA 1 cut(s) 231
Bsp143I GATC 5 cut(s) 29, 234, 288, 988, 1324
Bsp19I CCATGG 2 cut(s) 137, 1033
Bsp68I TCGCGA 1 cut(s) 567
BspACI CCGC 6 cut(s) 102, 107, 219, 223, 245, 1013
BspANI GGCC 5 cut(s) 85, 105, 136, 317, 428
BspCNI CTCAG 4 cut(s) 181, 535, 874, 1149
BspDI ATCGAT 1 cut(s) 381
BspEI TCCGGA 1 cut(s) 231
BspFNI CGCG 3 cut(s) 225, 567, 726
BspLI GGNNCC 4 cut(s) 60, 84, 236, 1384
BspPI GGATC 4 cut(s) 24, 229, 242, 1319
BspTNI GGTCTC 1 cut(s) 351
BsrFI RCCGGY 2 cut(s) 396, 761
BsrI ACTGG 3 cut(s) 544, 957, 967
BssAI RCCGGY 2 cut(s) 396, 761
BssECI CCNNGG 5 cut(s) 137, 423, 438, 1033, 1257
BssMI GATC 5 cut(s) 29, 234, 288, 988, 1324
BssT1I CCWWGG 3 cut(s) 137, 423, 1033
Bst2UI CCWGG 3 cut(s) 302, 440, 1259
Bst4CI ACNGT 2 cut(s) 97, 268
Bst6I CTCTTC 1 cut(s) 474
BstC8I GCNNGC 7 cut(s) 107, 192, 223, 398, 628, 1421, 1433
BstDEI CTNAG 5 cut(s) 168, 522, 637, 882, 1157
BstDSI CCRYGG 2 cut(s) 137, 1033
BstF5I GGATG 4 cut(s) 13, 790, 961, 1210
BstFNI CGCG 3 cut(s) 225, 567, 726
BstH2I RGCGCY 1 cut(s) 397
BstHHI GCGC 3 cut(s) 204, 331, 396
BstKTI GATC 5 cut(s) 32, 237, 291, 991, 1327
BstMAI GTCTC 2 cut(s) 351, 602
BstMBI GATC 5 cut(s) 29, 234, 288, 988, 1324
BstMCI CGRYCG 2 cut(s) 291, 766
BstMWI GCNNNNNNNGC 7 cut(s) 142, 425, 434, 677, 1099, 1351, 1380
BstNI CCWGG 3 cut(s) 302, 440, 1259
BstNSI RCATGY 1 cut(s) 615
BstSCI CCNGG 7 cut(s) 54, 65, 85, 300, 438, 675, 1257
BstSFI CTRYAG 1 cut(s) 1377
BstUI CGCG 3 cut(s) 225, 567, 726
BstV1I GCAGC 5 cut(s) 129, 234, 610, 1062, 1383
BstX2I RGATCY 1 cut(s) 234
BstYI RGATCY 1 cut(s) 234
Bsu15I ATCGAT 1 cut(s) 381
BsuRI GGCC 5 cut(s) 85, 105, 136, 317, 428
BsuTUI ATCGAT 1 cut(s) 381
BtgI CCRYGG 2 cut(s) 137, 1033
BtrI CACGTC 1 cut(s) 696
BtsCI GGATG 4 cut(s) 13, 790, 961, 1210
BtsIMutI CAGTG 2 cut(s) 950, 999
BtuMI TCGCGA 1 cut(s) 567
Cac8I GCNNGC 7 cut(s) 107, 192, 223, 398, 628, 1421, 1433
CfoI GCGC 3 cut(s) 204, 331, 396
Cfr10I RCCGGY 2 cut(s) 396, 761
Cfr13I GGNCC 4 cut(s) 58, 69, 83, 104
ClaI ATCGAT 1 cut(s) 381
CseI GACGC 1 cut(s) 715
Csp6I GTAC 5 cut(s) 131, 256, 581, 708, 1134
CspAI ACCGGT 1 cut(s) 761
CviAII CATG 9 cut(s) 91, 124, 138, 199, 430, 612, 1016, 1034, 1300
CviQI GTAC 5 cut(s) 131, 256, 581, 708, 1134
DdeI CTNAG 5 cut(s) 168, 522, 637, 882, 1157
DpnI GATC 5 cut(s) 31, 236, 290, 990, 1326
DpnII GATC 5 cut(s) 29, 234, 288, 988, 1324
DrdI GACNNNNNNGTC 1 cut(s) 76
DseDI GACNNNNNNGTC 1 cut(s) 76
EaeI YGGCCR 1 cut(s) 134
Eam1104I CTCTTC 1 cut(s) 474
EarI CTCTTC 1 cut(s) 474
Eco130I CCWWGG 3 cut(s) 137, 423, 1033
Eco31I GGTCTC 1 cut(s) 351
Eco47I GGWCC 2 cut(s) 58, 69
Eco57I CTGAAG 1 cut(s) 374
EcoRI GAATTC 1 cut(s) 349
EcoRII CCWGG 3 cut(s) 300, 438, 1257
EcoT14I CCWWGG 3 cut(s) 137, 423, 1033
ErhI CCWWGG 3 cut(s) 137, 423, 1033
FaeI CATG 9 cut(s) 94, 127, 141, 202, 433, 615, 1019, 1037, 1303
FalI AAGNNNNNCTT 2 cut(s) 731, 763
FaqI GGGAC 1 cut(s) 550
FatI CATG 9 cut(s) 90, 123, 137, 198, 429, 611, 1015, 1033, 1299
FauI CCCGC 5 cut(s) 114, 226, 230, 252, 1020
FblI GTMKAC 2 cut(s) 214, 263
Fnu4HI GCNGC 6 cut(s) 103, 143, 248, 624, 1051, 1372
FokI GGATG 3 cut(s) 797, 968, 1217
Fsp4HI GCNGC 6 cut(s) 103, 143, 248, 624, 1051, 1372
FspBI CTAG 4 cut(s) 665, 791, 803, 1047
FspI TGCGCA 1 cut(s) 330
GlaI GCGC 3 cut(s) 203, 330, 395
GluI GCNGC 6 cut(s) 103, 143, 248, 624, 1051, 1372
GsaI CCCAGC 1 cut(s) 516
GsuI CTGGAG 1 cut(s) 323
HaeII RGCGCY 1 cut(s) 397
HaeIII GGCC 5 cut(s) 85, 105, 136, 317, 428
HapII CCGG 9 cut(s) 39, 56, 67, 86, 205, 232, 397, 677, 762
HgaI GACGC 1 cut(s) 715
HhaI GCGC 3 cut(s) 204, 331, 396
Hin1II CATG 9 cut(s) 94, 127, 141, 202, 433, 615, 1019, 1037, 1303
Hin6I GCGC 3 cut(s) 202, 329, 394
HinP1I GCGC 3 cut(s) 202, 329, 394
HincII GTYRAC 2 cut(s) 264, 1245
HindII GTYRAC 2 cut(s) 264, 1245
HinfI GANTC 8 cut(s) 152, 370, 458, 562, 742, 934, 968, 1019
HpaII CCGG 9 cut(s) 39, 56, 67, 86, 205, 232, 397, 677, 762
HphI GGTGA 5 cut(s) 14, 379, 542, 665, 790
Hpy166II GTNNAC 6 cut(s) 10, 215, 264, 710, 1136, 1245
Hpy188I TCNGA 3 cut(s) 29, 185, 774
Hpy188III TCNNGA 7 cut(s) 232, 278, 354, 554, 566, 739, 1357
Hpy8I GTNNAC 6 cut(s) 10, 215, 264, 710, 1136, 1245
Hpy99I CGWCG 6 cut(s) 53, 101, 265, 268, 367, 700
HpyAV CCTTC 6 cut(s) 385, 527, 563, 571, 769, 977
HpyCH4III ACNGT 2 cut(s) 97, 268
HpyCH4IV ACGT 1 cut(s) 695
HpyCH4V TGCA 5 cut(s) 630, 1123, 1228, 1345, 1419
HpyF10VI GCNNNNNNNGC 7 cut(s) 142, 425, 434, 677, 1099, 1351, 1380
HpyF3I CTNAG 5 cut(s) 168, 522, 637, 882, 1157
HpySE526I ACGT 1 cut(s) 695
Hsp92II CATG 9 cut(s) 94, 127, 141, 202, 433, 615, 1019, 1037, 1303
HspAI GCGC 3 cut(s) 202, 329, 394
Kpn2I TCCGGA 1 cut(s) 231
KroI GCCGGC 1 cut(s) 396
KroNI GCCGGC 1 cut(s) 398
Kzo9I GATC 5 cut(s) 29, 234, 288, 988, 1324
LmnI GCTCC 6 cut(s) 150, 304, 661, 913, 1090, 1388
Lsp1109I GCAGC 5 cut(s) 129, 234, 610, 1062, 1383
LweI GCATC 1 cut(s) 946
MaeI CTAG 4 cut(s) 665, 791, 803, 1047
MaeII ACGT 1 cut(s) 695
MaeIII GTNAC 2 cut(s) 846, 1063
MalI GATC 5 cut(s) 31, 236, 290, 990, 1326
MboI GATC 5 cut(s) 29, 234, 288, 988, 1324
MboII GAAGA 4 cut(s) 127, 467, 491, 712
MflI RGATCY 1 cut(s) 234
MhlI GDGCHC 1 cut(s) 309
MluCI AATT 6 cut(s) 349, 715, 976, 1125, 1311, 1401
MluI ACGCGT 1 cut(s) 724
MlyI GAGTC 2 cut(s) 146, 751
MmeI TCCRAC 2 cut(s) 163, 1182
MroI TCCGGA 1 cut(s) 231
MroNI GCCGGC 1 cut(s) 396
MseI TTAA 2 cut(s) 297, 836
MspA1I CMGCKG 1 cut(s) 247
MspI CCGG 9 cut(s) 39, 56, 67, 86, 205, 232, 397, 677, 762
MspR9I CCNGG 7 cut(s) 56, 67, 87, 302, 440, 677, 1259
Mva1269I GAATGC 1 cut(s) 1347
MvaI CCWGG 3 cut(s) 302, 440, 1259
MvnI CGCG 3 cut(s) 225, 567, 726
MwoI GCNNNNNNNGC 7 cut(s) 142, 425, 434, 677, 1099, 1351, 1380
NaeI GCCGGC 1 cut(s) 398
NciI CCSGG 4 cut(s) 56, 67, 87, 677
NcoI CCATGG 2 cut(s) 137, 1033
NdeII GATC 5 cut(s) 29, 234, 288, 988, 1324
NgoMIV GCCGGC 1 cut(s) 396
NlaIII CATG 9 cut(s) 94, 127, 141, 202, 433, 615, 1019, 1037, 1303
NlaIV GGNNCC 4 cut(s) 60, 84, 236, 1384
NruI TCGCGA 1 cut(s) 567
NsbI TGCGCA 1 cut(s) 330
NspI RCATGY 1 cut(s) 615
PcsI WCGNNNNNNNCGW 1 cut(s) 260
PctI GAATGC 1 cut(s) 1347
PdiI GCCGGC 1 cut(s) 398
PfeI GAWTC 6 cut(s) 370, 458, 562, 934, 968, 1019
PflMI CCANNNNNTGG 1 cut(s) 1413
PinAI ACCGGT 1 cut(s) 761
PkrI GCNGC 6 cut(s) 104, 144, 249, 625, 1052, 1373
Ple19I CGATCG 1 cut(s) 291
PleI GAGTC 2 cut(s) 146, 750
PpsI GAGTC 2 cut(s) 146, 750
Psp6I CCWGG 3 cut(s) 300, 438, 1257
PspFI CCCAGC 1 cut(s) 512
PspGI CCWGG 3 cut(s) 300, 438, 1257
PspN4I GGNNCC 4 cut(s) 60, 84, 236, 1384
PspPI GGNCC 4 cut(s) 58, 69, 83, 104
PsuI RGATCY 1 cut(s) 234
PvuI CGATCG 1 cut(s) 291
RruI TCGCGA 1 cut(s) 567
RsaI GTAC 5 cut(s) 132, 257, 582, 709, 1135
RsaNI GTAC 5 cut(s) 131, 256, 581, 708, 1134
SalI GTCGAC 1 cut(s) 262
SaqAI TTAA 2 cut(s) 297, 836
SatI GCNGC 6 cut(s) 103, 143, 248, 624, 1051, 1372
Sau3AI GATC 5 cut(s) 29, 234, 288, 988, 1324
Sau96I GGNCC 4 cut(s) 58, 69, 83, 104
SchI GAGTC 2 cut(s) 146, 751
ScrFI CCNGG 7 cut(s) 56, 67, 87, 302, 440, 677, 1259
SduI GDGCHC 1 cut(s) 309
SfaNI GCATC 1 cut(s) 946
SfcI CTRYAG 1 cut(s) 1377
SgrDI CGTCGACG 1 cut(s) 262
SinI GGWCC 2 cut(s) 58, 69
Sse9I AATT 6 cut(s) 349, 715, 976, 1125, 1311, 1401
SsiI CCGC 6 cut(s) 102, 107, 219, 223, 245, 1013
SspI AATATT 1 cut(s) 860
SspMI CTAG 4 cut(s) 665, 791, 803, 1047
StyD4I CCNGG 7 cut(s) 54, 65, 85, 300, 438, 675, 1257
StyI CCWWGG 3 cut(s) 137, 423, 1033
TaaI ACNGT 2 cut(s) 97, 268
TaiI ACGT 1 cut(s) 698
TasI AATT 6 cut(s) 349, 715, 976, 1125, 1311, 1401
TatI WGTACW 1 cut(s) 707
TauI GCSGC 1 cut(s) 105
TfiI GAWTC 6 cut(s) 370, 458, 562, 934, 968, 1019
Tru1I TTAA 2 cut(s) 297, 836
Tru9I TTAA 2 cut(s) 297, 836
TscAI CASTG 2 cut(s) 957, 1006
TseI GCWGC 5 cut(s) 142, 247, 623, 1050, 1371
TspDTI ATGAA 8 cut(s) 367, 446, 660, 801, 911, 918, 1032, 1316
TspGWI ACGGA 4 cut(s) 127, 174, 719, 1375
TspRI CASTG 2 cut(s) 957, 1006
Van91I CCANNNNNTGG 1 cut(s) 1413
VpaK11BI GGWCC 2 cut(s) 58, 69
XapI RAATTY 2 cut(s) 349, 976
XceI RCATGY 1 cut(s) 615
XcmI CCANNNNNNNNNTGG 1 cut(s) 446
XmiI GTMKAC 2 cut(s) 214, 263
XspI CTAG 4 cut(s) 665, 791, 803, 1047
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.