Rh2DG240200

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000321
Physical Location & Seq
Reverse (-)
24338698 .. 24345418
6721 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG240200.1

Sequence Viewer

Length: 2244 bp
ATGCCTGCATGTAACACATCACTGAGATCAACCATTGTTGAGTTTTGCAGCTTGGAAAATTCAGGGATGGGTTTACCAAAAACCAAGAATGCCTCTGCTTCGGAGGAGACTGATGACAATATAACTGCCTCAATCGGGCTAAAAAGGAAACGTGGAAGAACAAGTATGGAGCGCATAGTGAACCGAGCACTTAGAGGTAAGAAATCAGTAGTTGAGTTTAACCCAAAGGGCGTACCATTTGGGAAGGCAGCAGCAGAGATGGCTTCTTACATAGGGGTGATTGTGCGTACCACGGTTCCCATCATTGTTGAGAGCTGGCCTAAGGTGGAGAAGGATCTGAAAAATGAAATTTGGAAATCCGTAGAGATGGCATTCGTCTTAGCTCCTAGATGCAGAAAAATGGTTTTATCATCTGCAGCCAATAAGTGGAGGCAATTCAAGTCAGAATTGACAACAAAATACGTACTGCCATATAAAGATCAGCCTGATGCTTTAAAAGACCCACCAGAAGAATATGATTTCATTAAGCAACAAGATTGGGAACAATTCGTAAAGAGTCGCTTAACCACAGATTTTCAGAAACTCCACATGGAGCAGAAAGAGAGAAGGGGAAAGTTGCAGAATGCTCATAGAATGTCACGAAAAGGATATGCTGGTTTAGAGGCAGAACTAAAAAAAACTATGAATGAAGATGAACTTGATCTAGCTGTCCTCTGGAAGAAAGGACGTGAGGACAAAAATGGGAATATCTCCCATGAGACTGTAGGGGAGCAGGCTGCAGAAATGGATACTTTAATGAACAATGAAGGGGATATTTCCAACTCTAACAGTGGATCAAGTGATGATGTCAGGTCAATGGGGTTGGGGACTCCAGAACATTCTAGTAGGGTTAGAGGTGCAGGAGAGTCTCTCATGCTAAATGTTTCACCTCCTCAGTTGTTGCGAGAAAGTGTCATGGATGAGGTCAGAAAGATGATAGAGCAGCAAAGGTTATGGTTTGATGCCAAGATTTCTTTGTTAGAGGCGAAGATCAGTGGTGATTGCCCAGCTGCAAGTATCACACTTCCAACTCCTCTGCTTGCCAAGCCTTCAAAGAAGGGCAGGTGTTCTGGGAAGACAAACGTTGAGGATAATGAAATTGATTCGGAAGCTTTCTCATTTGTGGGCAGAAGAGAATTCATGAAGGGAAAATCTTGCAAGTTGGCTGTTGGATCAATCAACAATGTTGTTTCCCATGGTACAATAATTGAAATGGATGTGGCGAACCACACAGTCCATGGTGTGCCTTTGGGAGAGGGCAATATACGTGTTGCTATTGATACTGCCCTAGATGAACAAGCGCTGCTTCCCATACCAGTTACAGGGGAATTAGCAACTGTTGGGCAAGCTGTAGGTTCTCATGTGGCTTGGCCGAAACATCTTGTTAAGTTGATGAATGAAGAGGAACGTGGAAATTCTTCTATAAAACCTCGAGATTTACCCAATCAAGATGTGATCCTGCCCAAGTCCCTCAAGCTCCTATACCGTTATGCTGAGCGTGCCATGACGGATGGTGAGCCTATCAGTGTCTTTATGGAAGAAGCCGTATTTGGTATTGCCAAGACTCTCAACATTTTCAAAGAAGATGTTATGCAATTTATGGAAATGAAGGAAATCCCCCCTAGGTGCATAACAGTGTACATGAGGCACCTCTATGATATGCTAAAGCAATCCAACATGGCCAATATTGTTGGTTTGATGGACCCTTCAAGCATATCAGTTGGGGAAGGCAATTCTGACCACAAATCACAGGTGCTCGCAACCCGGTTGCAGCAAGGGTCTGCTGACCAAATACTTTTGGTACCTTACAATTCTGGTTATCATTGGATGCTGACCATCATCAGTGAAGACAAAGAAGTATGCTACTTTATGGACCCTCTTCAACGCTATTTCATGGACCCTCTCCGAAGAAGCATGCGTGAGGAAGAATGGAAATATGTTGTAAACAATGGGATTAGACAATTCAACATTGAAACGGGTAGGGGATTCCGAAAACAACCTCTATGGAAAGTATTGATGGGTCCTAAACAACCCTCAAACATGGAGTGTGGGTATTATGTGATGAGATATATGAAAGAAATCATAGAAGGTCATGACCTTTCCTTTGCAATGAAGTGGGATGGGAGGAAGCTAAATGCCTACACACAGACAGAACTGGATGAGGTGAGGTGCGAGTGGACGGACTTTGTCAGTAATTATGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 Transposase_24

Protein Analysis

747

Amino Acids

84.5

Weight (kDa)

6.92

Isoelectric Point (pI)

42.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transposase_24 PF03004 178 - 301 6.6e-08 Plant transposase (Ptta/En/Spm family)
DUF8039 PF26133 395 - 477 5.6e-14 Domain of unknown function (DUF8039)
Peptidase_C48 PF02902 611 - 738 9e-07 Ulp1 protease family, C-terminal catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000321)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221 FvH4_1g20221
prunus_persica Prupe.6G157700_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1 Prupe.6G193600_v2.0.a1
pyrus_communis pycom03g09490 pycom03g19580 pycom07g21940 pycom07g27440 pycom14g09710
rosa_chinensis RchiOBHm_Chr1g0318231 RchiOBHm_Chr1g0318241 RchiOBHm_Chr1g0325001 RchiOBHm_Chr1g0325021 RchiOBHm_Chr2g0112301 RchiOBHm_Chr3g0482281 RchiOBHm_Chr3g0482291 RchiOBHm_Chr4g0390561 RchiOBHm_Chr4g0390571 RchiOBHm_Chr4g0417951 RchiOBHm_Chr7g0232611 RchiOBHm_Chr7g0240601
rosa_laevigata RLG00000001069 RLG00000013674 RLG00000018830 RLG00000020747
rosa_multiflora Rmu_co8364947.1_g000001 Rmu_co8416715.1_g000001 Rmu_sc0001348.1_g000014 Rmu_sc0002986.1_g000026 Rmu_sc0004003.1_g000007 Rmu_sc0005137.1_g000014 Rmu_sc0006583.1_g000016
rosa_roxburghii Rroxscaffold_159G00432800 Rroxscaffold_174G00435170 Rroxscaffold_1G00001870 Rroxscaffold_1G00044480 Rroxscaffold_2G00111880 Rroxscaffold_2G00111890 Rroxscaffold_2G00119670 Rroxscaffold_2G00129450 Rroxscaffold_2G00131520 Rroxscaffold_3G00240880 Rroxscaffold_5G00337440 Rroxscaffold_5G00356220 Rroxscaffold_5G00362390 Rroxscaffold_5G00368260 Rroxscaffold_7G00196230 Rroxscaffold_7G00196240 Rroxscaffold_7G00202020
rosa_rugosa Rorug01G0094700 Rorug02G0050500 Rorug02G0179800 Rorug05G0042800 Rorug05G0043000 Rorug05G0043100 Rorug05G0043200 Rorug06G0045300 Rorug06G0045600 Rorug06G0069400 Rorug07G0213000 Rorug07G0213000
rosa_samantha Rh1AG031600 Rh1DG131700 Rh1DG134800 Rh1DG288000 Rh1DG288100 Rh2AG218500 Rh2AG232500 Rh2BG245900 Rh2CG236700 Rh2DG224300 Rh2DG224400 Rh2DG240200 Rh3AG247300 Rh3BG126600 Rh3BG126700 Rh3BG333600 Rh5AG440500 Rh5AG440600 Rh5BG457800 Rh5DG472700 Rh5DG472800 Rh6BG138000 Rh6BG138100 Rh6BG171500 Rh6BG188400 Rh6BG230000 Rh6CG135600 Rh6CG135700 Rh6CG186300 Rh6CG450300 Rh7AG376100 Rh7CG394800 Rh7CG426100 Rh7CG426200 Rh7DG247700 Rh7DG322600 Rh7DG334300 Rh7DG364700
rosa_wichuraiana Rw2G018040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1092
Acc36I ACCTGC 1 cut(s) 1092
Acc65I GGTACC 1 cut(s) 1840
AccB1I GGYRCC 2 cut(s) 1686, 1840
AccB7I CCANNNNNTGG 1 cut(s) 426
AclI AACGTT 1 cut(s) 1122
AclWI GGATC 4 cut(s) 342, 841, 1219, 1489
AcoI YGGCCR 2 cut(s) 1409, 1719
AcsI RAATTY 4 cut(s) 58, 348, 1175, 1453
AfaI GTAC 6 cut(s) 234, 289, 465, 1240, 1679, 1842
AfeI AGCGCT 1 cut(s) 1341
AfiI CCNNNNNNNGG 3 cut(s) 135, 426, 1361
AflIII ACRYGT 1 cut(s) 1306
AgsI TTSAA 8 cut(s) 439, 1092, 1250, 1618, 1749, 1922, 2005, 2012
AjiI CACGTC 1 cut(s) 728
AjuI GAANNNNNNNTTGG 2 cut(s) 1572, 1604
AluBI AGCT 9 cut(s) 51, 315, 383, 707, 1049, 1151, 1388, 1516, 2170
AluI AGCT 9 cut(s) 51, 315, 383, 707, 1049, 1151, 1388, 1516, 2170
Alw21I GWGCWC 2 cut(s) 190, 1797
Alw26I GTCTC 3 cut(s) 101, 752, 912
AlwI GGATC 4 cut(s) 342, 841, 1219, 1489
Ama87I CYCGRG 1 cut(s) 1470
Aor51HI AGCGCT 1 cut(s) 1341
AoxI GGCC 3 cut(s) 317, 1409, 1719
ApeKI GCWGC 9 cut(s) 48, 248, 251, 416, 776, 982, 1049, 1342, 1810
ApoI RAATTY 4 cut(s) 58, 348, 1175, 1453
ArsI GACNNNNNNTTYG 2 cut(s) 2126, 2158
Asp700I GAANNNNTTC 1 cut(s) 1456
Asp718I GGTACC 1 cut(s) 1840
AspA2I CCTAGG 1 cut(s) 1661
AspLEI GCGC 2 cut(s) 174, 1342
AspS9I GGNCC 4 cut(s) 1741, 1912, 1936, 2060
AsuC2I CCSGG 1 cut(s) 1804
AsuHPI GGTGA 5 cut(s) 289, 918, 1049, 1565, 2215
AvaI CYCGRG 1 cut(s) 1470
AvaII GGWCC 4 cut(s) 1741, 1912, 1936, 2060
AvrII CCTAGG 1 cut(s) 1661
AxyI CCTNAGG 1 cut(s) 321
BalI TGGCCA 1 cut(s) 1721
BanI GGYRCC 2 cut(s) 1686, 1840
BbsI GAAGAC 2 cut(s) 1121, 1893
Bbv12I GWGCWC 2 cut(s) 190, 1797
BbvI GCAGC 9 cut(s) 60, 260, 263, 428, 763, 994, 1036, 1329, 1822
BccI CCATC 9 cut(s) 61, 253, 308, 361, 1544, 1732, 1883, 2050, 2153
BceAI ACGGC 1 cut(s) 1568
BciVI GTATCC 1 cut(s) 781
BcnI CCSGG 1 cut(s) 1804
BcoDI GTCTC 3 cut(s) 101, 752, 912
BfaI CTAG 5 cut(s) 387, 704, 882, 1328, 1662
BfmI CTRYAG 4 cut(s) 414, 762, 777, 1389
BfoI RGCGCY 1 cut(s) 1343
BfuAI ACCTGC 1 cut(s) 1092
BfuI GTATCC 1 cut(s) 781
BisI GCNGC 9 cut(s) 49, 249, 252, 417, 777, 983, 1050, 1343, 1811
BlnI CCTAGG 1 cut(s) 1661
BlpI GCTNAGC 1 cut(s) 1533
BlsI GCNGC 9 cut(s) 50, 250, 253, 418, 778, 984, 1051, 1344, 1812
Bme1390I CCNGG 1 cut(s) 1804
Bme18I GGWCC 4 cut(s) 1741, 1912, 1936, 2060
BmeT110I CYCGRG 1 cut(s) 1470
BmgBI CACGTC 1 cut(s) 728
BmgT120I GGNCC 4 cut(s) 1741, 1912, 1936, 2060
BmiI GGNNCC 7 cut(s) 297, 1688, 1743, 1842, 1914, 1938, 2061
BmrFI CCNGG 1 cut(s) 1804
BmsI GCATC 4 cut(s) 380, 478, 991, 1857
BpiI GAAGAC 2 cut(s) 1121, 1893
BplI GAGNNNNNCTC 2 cut(s) 894, 926
BpmI CTGGAG 1 cut(s) 855
Bpu1102I GCTNAGC 1 cut(s) 1533
BpuEI CTTGAG 1 cut(s) 1496
BpuMI CCSGG 1 cut(s) 1804
BsaAI YACGTR 2 cut(s) 463, 1307
BsaJI CCNNGG 4 cut(s) 291, 1234, 1276, 1661
BsaXI ACNNNNNCTCC 2 cut(s) 2075, 2105
Bsc4I CCNNNNNNNGG 3 cut(s) 135, 426, 1361
Bse1I ACTGG 2 cut(s) 1355, 2199
Bse21I CCTNAGG 1 cut(s) 321
Bse3DI GCAATG 1 cut(s) 2154
BseDI CCNNGG 4 cut(s) 291, 1234, 1276, 1661
BseGI GGATG 7 cut(s) 72, 964, 1261, 1555, 1872, 2164, 2203
BseLI CCNNNNNNNGG 3 cut(s) 135, 426, 1361
BseMI GCAATG 1 cut(s) 2154
BseMII CTCAG 3 cut(s) 14, 947, 1524
BseNI ACTGG 2 cut(s) 1355, 2199
BseRI GAGGAG 3 cut(s) 119, 921, 1062
BseXI GCAGC 9 cut(s) 60, 260, 263, 428, 763, 994, 1036, 1329, 1822
BseYI CCCAGC 1 cut(s) 1045
BsgI GTGCAG 1 cut(s) 918
BshFI GGCC 3 cut(s) 319, 1411, 1721
BshNI GGYRCC 2 cut(s) 1686, 1840
BsiHKAI GWGCWC 2 cut(s) 190, 1797
BsiHKCI CYCGRG 1 cut(s) 1470
BsiSI CCGG 1 cut(s) 1804
BslFI GGGAC 2 cut(s) 880, 1492
BslI CCNNNNNNNGG 3 cut(s) 135, 426, 1361
BsmAI GTCTC 3 cut(s) 101, 752, 912
BsmFI GGGAC 2 cut(s) 880, 1492
BsmI GAATGC 3 cut(s) 94, 371, 628
BsnI GGCC 3 cut(s) 319, 1411, 1721
BsoBI CYCGRG 1 cut(s) 1470
Bsp1286I GDGCHC 2 cut(s) 190, 1797
Bsp1407I TGTACA 1 cut(s) 1677
Bsp143I GATC 8 cut(s) 26, 334, 478, 700, 833, 1029, 1211, 1494
Bsp1720I GCTNAGC 1 cut(s) 1533
Bsp19I CCATGG 2 cut(s) 1234, 1276
BspANI GGCC 3 cut(s) 319, 1411, 1721
BspCNI CTCAG 3 cut(s) 15, 946, 1525
BspHI TCATGA 2 cut(s) 1179, 2131
BspLI GGNNCC 7 cut(s) 297, 1688, 1743, 1842, 1914, 1938, 2061
BspMAI CTGCAG 2 cut(s) 418, 781
BspMI ACCTGC 1 cut(s) 1092
BspPI GGATC 4 cut(s) 342, 841, 1219, 1489
BspT107I GGYRCC 2 cut(s) 1686, 1840
BsrDI GCAATG 1 cut(s) 2154
BsrGI TGTACA 1 cut(s) 1677
BsrI ACTGG 2 cut(s) 1355, 2199
BssECI CCNNGG 4 cut(s) 291, 1234, 1276, 1661
BssMI GATC 8 cut(s) 26, 334, 478, 700, 833, 1029, 1211, 1494
BssT1I CCWWGG 3 cut(s) 1234, 1276, 1661
Bst4CI ACNGT 7 cut(s) 295, 763, 830, 1273, 1378, 1526, 1675
Bst6I CTCTTC 3 cut(s) 1165, 1434, 1923
BstAUI TGTACA 1 cut(s) 1677
BstBAI YACGTR 2 cut(s) 463, 1307
BstC8I GCNNGC 8 cut(s) 6, 317, 774, 1080, 1386, 1539, 1797, 1955
BstDEI CTNAG 6 cut(s) 23, 191, 321, 379, 933, 1533
BstDSI CCRYGG 3 cut(s) 291, 1234, 1276
BstF5I GGATG 7 cut(s) 72, 964, 1261, 1555, 1872, 2164, 2203
BstH2I RGCGCY 1 cut(s) 1343
BstHHI GCGC 2 cut(s) 174, 1342
BstKTI GATC 8 cut(s) 29, 337, 481, 703, 836, 1032, 1214, 1497
BstMAI GTCTC 3 cut(s) 101, 752, 912
BstMBI GATC 8 cut(s) 26, 334, 478, 700, 833, 1029, 1211, 1494
BstMWI GCNNNNNNNGC 3 cut(s) 260, 1084, 1538
BstNSI RCATGY 2 cut(s) 12, 1957
BstSCI CCNGG 1 cut(s) 1802
BstSFI CTRYAG 4 cut(s) 414, 762, 777, 1389
BstSNI TACGTA 1 cut(s) 463
BstV1I GCAGC 9 cut(s) 60, 260, 263, 428, 763, 994, 1036, 1329, 1822
BstV2I GAAGAC 2 cut(s) 1121, 1893
BstX2I RGATCY 1 cut(s) 334
BstYI RGATCY 1 cut(s) 334
Bsu36I CCTNAGG 1 cut(s) 321
BsuI GTATCC 1 cut(s) 781
BsuRI GGCC 3 cut(s) 319, 1411, 1721
BtgI CCRYGG 3 cut(s) 291, 1234, 1276
BtrI CACGTC 1 cut(s) 728
BtsCI GGATG 7 cut(s) 72, 964, 1261, 1555, 1872, 2164, 2203
BtsIMutI CAGTG 6 cut(s) 20, 835, 1039, 1570, 1680, 1888
BveI ACCTGC 1 cut(s) 1092
Cac8I GCNNGC 8 cut(s) 6, 317, 774, 1080, 1386, 1539, 1797, 1955
CciI TCATGA 2 cut(s) 1179, 2131
CfoI GCGC 2 cut(s) 174, 1342
Cfr13I GGNCC 4 cut(s) 1741, 1912, 1936, 2060
Csp6I GTAC 6 cut(s) 233, 288, 464, 1239, 1678, 1841
CviQI GTAC 6 cut(s) 233, 288, 464, 1239, 1678, 1841
DdeI CTNAG 6 cut(s) 23, 191, 321, 379, 933, 1533
DpnI GATC 8 cut(s) 28, 336, 480, 702, 835, 1031, 1213, 1496
DpnII GATC 8 cut(s) 26, 334, 478, 700, 833, 1029, 1211, 1494
DraI TTTAAA 1 cut(s) 495
EaeI YGGCCR 2 cut(s) 1409, 1719
Eam1104I CTCTTC 3 cut(s) 1165, 1434, 1923
EarI CTCTTC 3 cut(s) 1165, 1434, 1923
Eco105I TACGTA 1 cut(s) 463
Eco130I CCWWGG 3 cut(s) 1234, 1276, 1661
Eco47I GGWCC 4 cut(s) 1741, 1912, 1936, 2060
Eco47III AGCGCT 1 cut(s) 1341
Eco81I CCTNAGG 1 cut(s) 321
Eco88I CYCGRG 1 cut(s) 1470
EcoO109I RGGNCCY 1 cut(s) 2060
EcoRI GAATTC 1 cut(s) 1175
EcoT14I CCWWGG 3 cut(s) 1234, 1276, 1661
ErhI CCWWGG 3 cut(s) 1234, 1276, 1661
FalI AAGNNNNNCTT 6 cut(s) 545, 577, 681, 713, 1329, 1361
FaqI GGGAC 2 cut(s) 880, 1492
Fnu4HI GCNGC 9 cut(s) 49, 249, 252, 417, 777, 983, 1050, 1343, 1811
FokI GGATG 7 cut(s) 79, 971, 1268, 1562, 1879, 2171, 2210
Fsp4HI GCNGC 9 cut(s) 49, 249, 252, 417, 777, 983, 1050, 1343, 1811
FspBI CTAG 5 cut(s) 387, 704, 882, 1328, 1662
GlaI GCGC 2 cut(s) 173, 1341
GluI GCNGC 9 cut(s) 49, 249, 252, 417, 777, 983, 1050, 1343, 1811
GsaI CCCAGC 1 cut(s) 1049
GsuI CTGGAG 1 cut(s) 855
HaeII RGCGCY 1 cut(s) 1343
HaeIII GGCC 3 cut(s) 319, 1411, 1721
HapII CCGG 1 cut(s) 1804
HhaI GCGC 2 cut(s) 174, 1342
Hin6I GCGC 2 cut(s) 172, 1340
HinP1I GCGC 2 cut(s) 172, 1340
HindIII AAGCTT 1 cut(s) 1149
HinfI GANTC 6 cut(s) 556, 868, 905, 1142, 1603, 2025
HpaII CCGG 1 cut(s) 1804
HphI GGTGA 5 cut(s) 289, 918, 1049, 1565, 2215
Hpy166II GTNNAC 5 cut(s) 74, 181, 1678, 1984, 2217
Hpy188I TCNGA 9 cut(s) 103, 339, 445, 579, 968, 1147, 1777, 1946, 2030
Hpy188III TCNNGA 7 cut(s) 639, 715, 872, 1180, 1472, 1487, 2132
Hpy8I GTNNAC 5 cut(s) 74, 181, 1678, 1984, 2217
HpyCH4III ACNGT 7 cut(s) 295, 763, 830, 1273, 1378, 1526, 1675
HpyCH4IV ACGT 6 cut(s) 151, 462, 727, 1122, 1306, 1447
HpyF10VI GCNNNNNNNGC 3 cut(s) 260, 1084, 1538
HpyF3I CTNAG 6 cut(s) 23, 191, 321, 379, 933, 1533
HpySE526I ACGT 6 cut(s) 151, 462, 727, 1122, 1306, 1447
HspAI GCGC 2 cut(s) 172, 1340
KpnI GGTACC 1 cut(s) 1844
Kzo9I GATC 8 cut(s) 26, 334, 478, 700, 833, 1029, 1211, 1494
LmnI GCTCC 5 cut(s) 169, 388, 592, 769, 1521
Lsp1109I GCAGC 9 cut(s) 60, 260, 263, 428, 763, 994, 1036, 1329, 1822
LweI GCATC 4 cut(s) 380, 478, 991, 1857
MaeI CTAG 5 cut(s) 387, 704, 882, 1328, 1662
MaeII ACGT 6 cut(s) 151, 462, 727, 1122, 1306, 1447
MaeIII GTNAC 3 cut(s) 11, 636, 1357
MalI GATC 8 cut(s) 28, 336, 480, 702, 835, 1031, 1213, 1496
MboI GATC 8 cut(s) 26, 334, 478, 700, 833, 1029, 1211, 1494
MflI RGATCY 1 cut(s) 334
MhlI GDGCHC 2 cut(s) 190, 1797
MlsI TGGCCA 1 cut(s) 1721
MluNI TGGCCA 1 cut(s) 1721
MlyI GAGTC 4 cut(s) 565, 862, 914, 1597
MmeI TCCRAC 4 cut(s) 843, 1091, 1189, 1737
Mox20I TGGCCA 1 cut(s) 1721
MroXI GAANNNNTTC 1 cut(s) 1456
MscI TGGCCA 1 cut(s) 1721
MseI TTAA 6 cut(s) 219, 494, 525, 563, 794, 1425
MslI CAYNNNNRTG 3 cut(s) 275, 1673, 1692
Msp20I TGGCCA 1 cut(s) 1721
MspA1I CMGCKG 1 cut(s) 1049
MspI CCGG 1 cut(s) 1804
MspR9I CCNGG 1 cut(s) 1804
Mva1269I GAATGC 3 cut(s) 94, 371, 628
MwoI GCNNNNNNNGC 3 cut(s) 260, 1084, 1538
NciI CCSGG 1 cut(s) 1804
NcoI CCATGG 2 cut(s) 1234, 1276
NdeII GATC 8 cut(s) 26, 334, 478, 700, 833, 1029, 1211, 1494
NlaIV GGNNCC 7 cut(s) 297, 1688, 1743, 1842, 1914, 1938, 2061
NmuCI GTSAC 1 cut(s) 636
NspI RCATGY 2 cut(s) 12, 1957
PaeI GCATGC 1 cut(s) 1957
PaeR7I CTCGAG 1 cut(s) 1470
PagI TCATGA 2 cut(s) 1179, 2131
PaqCI CACCTGC 1 cut(s) 1092
PctI GAATGC 3 cut(s) 94, 371, 628
PdmI GAANNNNTTC 1 cut(s) 1456
PfeI GAWTC 2 cut(s) 1142, 2025
PflFI GACNNNGTC 1 cut(s) 2225
PflMI CCANNNNNTGG 1 cut(s) 426
PkrI GCNGC 9 cut(s) 50, 250, 253, 418, 778, 984, 1051, 1344, 1812
PleI GAGTC 4 cut(s) 564, 862, 913, 1597
PpsI GAGTC 4 cut(s) 564, 862, 913, 1597
Ppu21I YACGTR 2 cut(s) 463, 1307
PpuMI RGGWCCY 1 cut(s) 2060
Psp1406I AACGTT 1 cut(s) 1122
Psp5II RGGWCCY 1 cut(s) 2060
PspFI CCCAGC 1 cut(s) 1045
PspN4I GGNNCC 7 cut(s) 297, 1688, 1743, 1842, 1914, 1938, 2061
PspPI GGNCC 4 cut(s) 1741, 1912, 1936, 2060
PspPPI RGGWCCY 1 cut(s) 2060
PstI CTGCAG 2 cut(s) 418, 781
PsuI RGATCY 1 cut(s) 334
PsyI GACNNNGTC 1 cut(s) 2225
PvuII CAGCTG 1 cut(s) 1049
RsaI GTAC 6 cut(s) 234, 289, 465, 1240, 1679, 1842
RsaNI GTAC 6 cut(s) 233, 288, 464, 1239, 1678, 1841
RseI CAYNNNNRTG 3 cut(s) 275, 1673, 1692
SaqAI TTAA 6 cut(s) 219, 494, 525, 563, 794, 1425
SatI GCNGC 9 cut(s) 49, 249, 252, 417, 777, 983, 1050, 1343, 1811
Sau3AI GATC 8 cut(s) 26, 334, 478, 700, 833, 1029, 1211, 1494
Sau96I GGNCC 4 cut(s) 1741, 1912, 1936, 2060
SchI GAGTC 4 cut(s) 565, 862, 914, 1597
ScrFI CCNGG 1 cut(s) 1804
SduI GDGCHC 2 cut(s) 190, 1797
SfaNI GCATC 4 cut(s) 380, 478, 991, 1857
SfcI CTRYAG 4 cut(s) 414, 762, 777, 1389
Sfr274I CTCGAG 1 cut(s) 1470
SinI GGWCC 4 cut(s) 1741, 1912, 1936, 2060
SlaI CTCGAG 1 cut(s) 1470
SmiMI CAYNNNNRTG 3 cut(s) 275, 1673, 1692
SmlI CTYRAG 2 cut(s) 1470, 1511
SmoI CTYRAG 2 cut(s) 1470, 1511
SnaBI TACGTA 1 cut(s) 463
SphI GCATGC 1 cut(s) 1957
SspI AATATT 1 cut(s) 1726
SspMI CTAG 5 cut(s) 387, 704, 882, 1328, 1662
StyD4I CCNGG 1 cut(s) 1802
StyI CCWWGG 3 cut(s) 1234, 1276, 1661
TaaI ACNGT 7 cut(s) 295, 763, 830, 1273, 1378, 1526, 1675
TaiI ACGT 6 cut(s) 154, 465, 730, 1125, 1309, 1450
TaqI TCGA 1 cut(s) 1471
TatI WGTACW 1 cut(s) 1677
TfiI GAWTC 2 cut(s) 1142, 2025
Tru1I TTAA 6 cut(s) 219, 494, 525, 563, 794, 1425
Tru9I TTAA 6 cut(s) 219, 494, 525, 563, 794, 1425
TscAI CASTG 6 cut(s) 27, 835, 1039, 1570, 1680, 1888
TseFI GTSAC 1 cut(s) 636
TseI GCWGC 9 cut(s) 48, 248, 251, 416, 776, 982, 1049, 1342, 1810
Tsp45I GTSAC 1 cut(s) 636
TspGWI ACGGA 3 cut(s) 349, 1562, 2234
TspRI CASTG 6 cut(s) 27, 835, 1039, 1570, 1680, 1888
Tth111I GACNNNGTC 1 cut(s) 2225
Van91I CCANNNNNTGG 1 cut(s) 426
VpaK11BI GGWCC 4 cut(s) 1741, 1912, 1936, 2060
XapI RAATTY 4 cut(s) 58, 348, 1175, 1453
XceI RCATGY 2 cut(s) 12, 1957
XcmI CCANNNNNNNNNTGG 1 cut(s) 1274
XhoI CTCGAG 1 cut(s) 1470
XmaJI CCTAGG 1 cut(s) 1661
XmnI GAANNNNTTC 1 cut(s) 1456
XspI CTAG 5 cut(s) 387, 704, 882, 1328, 1662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.