FvH4_3g09240

Protein of unknown function (DUF2921)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000420
Physical Location & Seq
Forward (+)
5401875 .. 5404992
3118 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g09240.t1

Sequence Viewer

Length: 2880 bp
ATGGAGACCAATCCCCCATCCTTCCCCAAATCTAGGGTTAGGGCTTTCCCAATTTCCCCAACCACTCCTTCACAAACCCATAAATCCCAATCCACTCCCCTCCTCCTCCTCTTCTTCCTCCTCATCAATCTCACCCCCACAGCTTCACTCCTCAACCAAATCCCCAATCTTGATCACTTATACTCCGACCACTGCAACGACGTCGTTCCCAAATCGGAGACCCACCCAAAATGGTTCCTCACCACCCTCTCCGTCCAAGACATCGGATTCCACAGCAGCTACTTCACCGGCGGCGACCGGTTCTTCAATCACACCTCCGGCGACGACAGTAAAGCTCTCATCTTTCGGCCCAACTTCGTTGCCAGAACCCTGAAAGATGGAATCTTCCACGTCAGAGGGAGTATGGATCTTCAGTCTCTTTTGGGGGATTCGACTCGCCGGAATCTGAAGCTGGTAAACTTCCGGGGACACAGATTTCCGGTCAGGTGGGGCGTGGTGAGGTTCAGGCTTGATGGGTATTATTCAGAATCGGATAAGAAGCTGTGTATGATTGGGAAGAGTAGGTCCATGTATAATGCAGGTAACTTCGAGCCTAGTGTTGTTCTTAAGCTTAATTATCCGAAATGGTCTAGTATTTATGGTAGTTTGATTAGTGGGAGCTTAGGGAGTGTTGTTAGTGATAAGGATGATGGTGAGAATTATTTTGATGCTATTAGGGTGTTGGCTTTTTCTGTGAAGCATAGTTATGGGTATACATTTGTTGGGGAAGATAGGATGAGTAGTTTTCCGAGTAGTGTTGAGAGTGGAGAGAGTATAGGTTTGAGGAATTTGAATGATTCGAACGGTGGTTTGTGTAGGTTGCTTGGTAACCGTCTCGAGAGATTTGAACTGGTGTATGGGAGTGAGTGTGGTGGGGGTGTGAATTGCAATCCTCTTGGTGGGGATGTTGGATATGTACCGAGCTCGTTGGTTTTTTATGGGACTCGGTGTACAGAAGGGAGGAAAATGCAGATGCTGTTGGGGTTTTCCAACTCTAGTGGAGATGAATTCTTGAGATTTCCATTTGTACCTAGCACCACGTTGATTGCGGAAGGGGAATGGGATGAGAAGGGGAATCGTCTATGTGCAGTTGCATGTAGGATTTTGAATTTTACAGAGTCTTTGACTGGTGCACTTGTTGGAGACTGCTCAACTAAACTCAGCTTGAGATTTCCGGTGAGGTTATCTTTACAAAATAGGTCTGCTCTTGTGGGGCAAATTTGGAGCGACAAAAAAATGAGTGATTTAGGTTACTTCAGTAAAATTGGGTTCCAAGGTCTGTCAGGATGGTTAGTGAAACTCTTAGGATACAAATATGAATATTCTGTGACTGGCAACCTGAGAAAAACATGTGTGGAAAAGAGGGCTGGGATAGGCAAAGGAAAGAAATACCCTGAGGAGTATTCATCAGATATGAAATTTGACATGAGAGTGAGCAACAGCAGAGGACAAGTTGCGTGGGGTTATTCGTCCCCATTATTTGTTGATGATGACCGGATTTATGGACGCCGGTTTTGGGATAAACCACAGGAAACAGAAGTACCAAGTCGTCAGAAGAAAAGCCATAGCAGTCCAATGAACATTAGCTATAAGTTGAGCTTCACACGTGGTGCCCAATTTTTTCATGATGTCTTCCCATCTAAAGCAGAACTAGCTGCTGAAGGAATATATGATACAGATTACGGAAACCTATGCATGATAGGATGTAGGCATGTATCATCCAAGGAACAAAACTCGATTAACAAGGATATGTTAGACTGTTCTGTTAAAATTGATGTACACTTTCCTCCATTGGATACCAAGGATGGCCAGAATGTCAAGGGAATTATCGAAAGCATGAGGAATAAGTCAGACCCTCTATATTTTGATCATATTGAATTCTCTTCAACTTCAATTTACCATAACCAAGCTGCTGCTTCCATTACCAGAATTGATTTTGAGATTGTTATGGTTTTGATTTCTAATACCCTTATATGTGCTTTTGTGGGATGGCAACTCTATTTTGTGAAGAAAAACCCAGATGTGCTCCCATTCACTTCGCTTGTCATGCTCACTGTTCTTACTCTGGGATATGTGATTCCTCTTCTGTTCACCTTTGAGGCAATGCTTGCACCAAACCAAAGCAAGCAGAGTACTTTTCTTGGGATCGGTGGATGGCTCCAGGTTAATGAAGTAATGGTCAGACTGATATTGTTGGTAACTTTCATTTTGCACCTGCTTCTTCTTCAACTGACCTTGTCATCAAGACAAGGTGTTAATAGCCAGAAGAGCTTGATGGATTCTGAGAGGAAGGTTCTTTATGCCACATTAACACTGTACATAACTGGCGCACTGATTACTTGTTCTGTCCACCAGTGGATGAGTACTCACCAGAAATCATACAGGCCATTTCATCGCAACCTTCGGCGAGTGACTAACATGTGGCATCTTGTTCACCCGCAACGCTCTCTGTGGGATGACCTCAAGTCGTATGCTGGATTCATCCTGGATGGTTTTCTGCTCCCACAAATATTGTTCAACTTACTTGTGAATTCAGGTGAAAGGGCTCTGTCCTGTGCCTTTTACTTTGGAACCACTATGATTCGTCTGCTGCCTCATGCTTATGATCTGTACAGAGCTCGCGCTTACACACGGTTACTCGCTTTCTCATATATTTATGCAAACCCAAAAACAGATTTTTTCTCCACGGCCTGGAACATAATCATATCCTGTTGTTGTCTGCTGTTTGCTGCCATTATTTTCTTGCAGCAAAGGTGTGGCGGCCGTTGTATTCTTCCCAAAATATTTAGACATTCCACTGTATATGAAAAGGTCCCTGTCATCAGCAATGAGGAATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
DUF2921

Protein Analysis

960

Amino Acids

108.39

Weight (kDa)

9.05

Isoelectric Point (pI)

42.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2921_N PF25333 61 - 238 1.8e-18 DUF2921 lipocalin-like domain
DUF2921_N PF25333 296 - 435 4.3e-28 DUF2921 lipocalin-like domain
DUF2921_N PF25333 472 - 638 9.7e-32 DUF2921 lipocalin-like domain
DUF2921 PF11145 650 - 942 3.8e-55 Transmembrane E3 ligase/DUF2921 transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21700
fragaria_vesca FvH4_1g10360 FvH4_1g10760 FvH4_2g30370 FvH4_3g09240 FvH4_3g20050 FvH4_3g20280 FvH4_3g20290 FvH4_3g20291 FvH4_3g20340 FvH4_3g20350 FvH4_3g20351 FvH4_3g20380 FvH4_3g20390 FvH4_5g13190 FvH4_6g20070 FvH4_6g42890
malus_domestica MD03G1227000.v1.1 MD10G1294900.v1.1 MD11G1246300.v1.1 MD11G1246400.v1.1 MD11G1246500.v1.1 MD11G1246600.v1.1
prunus_persica Prupe.4G047300_v2.0.a1 Prupe.4G181700_v2.0.a1 Prupe.4G181900_v2.0.a1 Prupe.4G182000_v2.0.a1 Prupe.4G182200_v2.0.a1
pyrus_communis pycom03g17470 pycom10g24670 pycom11g21640
rosa_chinensis RchiOBHm_Chr5g0008381 RchiOBHm_Chr5g0008391 RchiOBHm_Chr5g0033881 RchiOBHm_Chr5g0034171 RchiOBHm_Chr5g0034181 RchiOBHm_Chr5g0034191 RchiOBHm_Chr5g0034201 RchiOBHm_Chr5g0034211 RchiOBHm_Chr5g0034221 RchiOBHm_Chr5g0034231 RchiOBHm_Chr5g0034271 RchiOBHm_Chr5g0034281
rosa_laevigata RLG00000031566 RLG00000031567 RLG00000033506 RLG00000033529 RLG00000033530 RLG00000033531
rosa_multiflora Rmu_co8445509.1_g000001 Rmu_co8470615.1_g000001 Rmu_sc0008805.1_g000003 Rmu_sc0008805.1_g000004 Rmu_ssc0000128.1_g000029 Rmu_ssc0000128.1_g000031 Rmu_ssc0000128.1_g000032 Rmu_ssc0000388.1_g000062 Rmu_ssc0000388.1_g000063
rosa_roxburghii Rroxscaffold_1G00046010 Rroxscaffold_1G00046020 Rroxscaffold_1G00046030 Rroxscaffold_1G00046040 Rroxscaffold_1G00046060 Rroxscaffold_1G00046070 Rroxscaffold_1G00046080 Rroxscaffold_1G00046090
rosa_rugosa Rorug04G0435500 Rorug05G0142900 Rorug05G0144600 Rorug05G0144600 Rorug05G0144700 Rorug05G0144800
rosa_samantha Rh5AG233800 Rh5AG235900 Rh5AG236000 Rh5AG236100 Rh5AG236200 Rh5AG236300 Rh5AG236500 Rh5BG061200 Rh5BG061300 Rh5BG234300 Rh5BG236600 Rh5BG236800 Rh5CG071200 Rh5DG060000 Rh5DG060100 Rh5DG241300 Rh5DG243700 Rh5DG244000 Rh5DG244100
rosa_wichuraiana Rw3G018950 Rw5G005680 Rw5G021330 Rw5G021340 Rw5G021350 Rw5G021550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2262
AatII GACGTC 1 cut(s) 204
Acc36I ACCTGC 2 cut(s) 569, 2262
AccB1I GGYRCC 1 cut(s) 1649
AccB7I CCANNNNNTGG 1 cut(s) 2730
AccI GTMKAC 1 cut(s) 752
AccII CGCG 1 cut(s) 2661
AciI CCGC 4 cut(s) 291, 1088, 2477, 2799
AclWI GGATC 2 cut(s) 414, 2192
AcoI YGGCCR 2 cut(s) 1846, 2800
AcsI RAATTY 7 cut(s) 826, 1046, 1147, 1257, 1457, 1916, 2569
AcuI CTGAAG 4 cut(s) 395, 467, 1279, 1719
AcvI CACGTG 1 cut(s) 1646
AcyI GRCGYC 2 cut(s) 201, 1546
AdeI CACNNNGTG 1 cut(s) 1649
AfaI GTAC 9 cut(s) 957, 991, 1068, 1581, 1818, 2173, 2357, 2404, 2651
AfiI CCNNNNNNNGG 4 cut(s) 33, 938, 1555, 2730
AflII CTTAAG 1 cut(s) 605
AflIII ACRYGT 3 cut(s) 1388, 1643, 2457
AgeI ACCGGT 1 cut(s) 297
AgsI TTSAA 9 cut(s) 307, 832, 887, 1147, 1916, 1926, 1932, 2267, 2557
AhdI GACNNNNNGTC 1 cut(s) 2503
AjiI CACGTC 1 cut(s) 391
AjnI CCWGG 3 cut(s) 2199, 2523, 2729
AjuI GAANNNNNNNTTGG 2 cut(s) 52, 84
Alw21I GWGCWC 4 cut(s) 965, 1174, 2067, 2659
Alw26I GTCTC 4 cut(s) 212, 420, 878, 1176
Alw44I GTGCAC 1 cut(s) 1170
AlwI GGATC 2 cut(s) 414, 2192
AlwNI CAGNNNCTG 1 cut(s) 1015
Ama87I CYCGRG 1 cut(s) 875
AoxI GGCC 5 cut(s) 347, 1846, 2423, 2727, 2800
ApaLI GTGCAC 1 cut(s) 1170
ApeKI GCWGC 7 cut(s) 276, 1694, 1949, 1952, 2629, 2768, 2785
ApoI RAATTY 7 cut(s) 826, 1046, 1147, 1257, 1457, 1916, 2569
ArsI GACNNNNNNTTYG 2 cut(s) 1536, 1568
AsiGI ACCGGT 1 cut(s) 297
AspLEI GCGC 2 cut(s) 2369, 2663
AspS9I GGNCC 3 cut(s) 348, 564, 2851
AsuC2I CCSGG 1 cut(s) 464
AsuII TTCGAA 1 cut(s) 839
AvaI CYCGRG 1 cut(s) 875
AvaII GGWCC 2 cut(s) 564, 2851
AxyI CCTNAGG 1 cut(s) 1434
BaeGI GKGCMC 2 cut(s) 1174, 1654
BaeI ACNNNNGTAYC 2 cut(s) 1563, 1596
BalI TGGCCA 1 cut(s) 1848
BanI GGYRCC 1 cut(s) 1649
BanII GRGCYC 3 cut(s) 965, 2587, 2659
BbrPI CACGTG 1 cut(s) 1646
BbsI GAAGAC 1 cut(s) 1663
Bbv12I GWGCWC 4 cut(s) 965, 1174, 2067, 2659
BbvI GCAGC 7 cut(s) 288, 1681, 1936, 1939, 2616, 2755, 2797
BceAI ACGGC 2 cut(s) 2742, 2787
BcgI CGANNNNNNTGC 2 cut(s) 184, 218
BciT130I CCWGG 3 cut(s) 2201, 2525, 2731
BciVI GTATCC 2 cut(s) 1340, 1828
BclI TGATCA 2 cut(s) 172, 1906
BcnI CCSGG 1 cut(s) 464
BcoDI GTCTC 4 cut(s) 212, 420, 878, 1176
BfaI CTAG 6 cut(s) 33, 594, 630, 1035, 1071, 1691
BfrI CTTAAG 1 cut(s) 605
BfuAI ACCTGC 2 cut(s) 569, 2262
BfuI GTATCC 2 cut(s) 1340, 1828
BisI GCNGC 9 cut(s) 277, 292, 1695, 1950, 1953, 2630, 2769, 2786, 2800
BlsI GCNGC 9 cut(s) 278, 293, 1696, 1951, 1954, 2631, 2770, 2787, 2801
BmcAI AGTACT 2 cut(s) 2173, 2404
Bme1390I CCNGG 4 cut(s) 464, 2201, 2525, 2731
Bme18I GGWCC 2 cut(s) 564, 2851
BmeRI GACNNNNNGTC 1 cut(s) 2503
BmeT110I CYCGRG 1 cut(s) 875
BmgBI CACGTC 1 cut(s) 391
BmgT120I GGNCC 3 cut(s) 348, 564, 2851
BmiI GGNNCC 6 cut(s) 236, 1310, 1651, 2198, 2611, 2853
BmrFI CCNGG 4 cut(s) 464, 2201, 2525, 2731
BmsI GCATC 3 cut(s) 697, 1002, 2473
BpiI GAAGAC 1 cut(s) 1663
BpmI CTGGAG 1 cut(s) 2183
Bpu10I CCTNAGC 1 cut(s) 661
Bpu14I TTCGAA 1 cut(s) 839
BpuEI CTTGAG 3 cut(s) 1072, 1225, 2486
BpuMI CCSGG 1 cut(s) 464
BsaAI YACGTR 1 cut(s) 1646
BsaHI GRCGYC 2 cut(s) 201, 1546
BsaI GGTCTC 1 cut(s) 212
BsaJI CCNNGG 5 cut(s) 463, 1312, 1761, 1839, 2724
BsaWI WCCGGW 4 cut(s) 297, 478, 1213, 1533
BsaXI ACNNNNNCTCC 4 cut(s) 167, 197, 892, 922
Bsc4I CCNNNNNNNGG 4 cut(s) 33, 938, 1555, 2730
Bse118I RCCGGY 3 cut(s) 287, 297, 1548
Bse1I ACTGG 5 cut(s) 894, 1171, 1375, 2368, 2392
Bse21I CCTNAGG 1 cut(s) 1434
Bse3DI GCAATG 2 cut(s) 2148, 2872
BseBI CCWGG 3 cut(s) 2201, 2525, 2731
BseDI CCNNGG 5 cut(s) 463, 1312, 1761, 1839, 2724
BseLI CCNNNNNNNGG 4 cut(s) 33, 938, 1555, 2730
BseMI GCAATG 2 cut(s) 2148, 2872
BseMII CTCAG 4 cut(s) 1213, 1370, 1425, 2313
BseNI ACTGG 5 cut(s) 894, 1171, 1375, 2368, 2392
BseRI GAGGAG 6 cut(s) 92, 95, 98, 110, 140, 1451
BseSI GKGCMC 2 cut(s) 1174, 1654
BseX3I CGGCCG 1 cut(s) 2800
BseXI GCAGC 7 cut(s) 288, 1681, 1936, 1939, 2616, 2755, 2797
BseYI CCCAGC 1 cut(s) 1406
BsgI GTGCAG 1 cut(s) 1146
Bsh1236I CGCG 1 cut(s) 2661
Bsh1285I CGRYCG 2 cut(s) 298, 2803
BshFI GGCC 5 cut(s) 349, 1848, 2425, 2729, 2802
BshNI GGYRCC 1 cut(s) 1649
BshTI ACCGGT 1 cut(s) 297
BsiEI CGRYCG 2 cut(s) 298, 2803
BsiHKAI GWGCWC 4 cut(s) 965, 1174, 2067, 2659
BsiHKCI CYCGRG 1 cut(s) 875
BsiSI CCGG 9 cut(s) 288, 298, 318, 439, 463, 479, 1214, 1534, 1549
BslFI GGGAC 4 cut(s) 480, 994, 1495, 2837
BslI CCNNNNNNNGG 4 cut(s) 33, 938, 1555, 2730
BsmAI GTCTC 4 cut(s) 212, 420, 878, 1176
BsmBI CGTCTC 1 cut(s) 878
BsmFI GGGAC 4 cut(s) 480, 994, 1495, 2837
BsnI GGCC 5 cut(s) 349, 1848, 2425, 2729, 2802
Bso31I GGTCTC 1 cut(s) 212
BsoBI CYCGRG 1 cut(s) 875
Bsp119I TTCGAA 1 cut(s) 839
Bsp1286I GDGCHC 6 cut(s) 965, 1174, 1654, 2067, 2587, 2659
Bsp1407I TGTACA 4 cut(s) 989, 1816, 2355, 2649
Bsp143I GATC 5 cut(s) 172, 406, 1906, 2184, 2644
BspACI CCGC 4 cut(s) 291, 1088, 2477, 2799
BspANI GGCC 5 cut(s) 349, 1848, 2425, 2729, 2802
BspCNI CTCAG 4 cut(s) 1212, 1371, 1426, 2314
BspFNI CGCG 1 cut(s) 2661
BspHI TCATGA 1 cut(s) 1663
BspLI GGNNCC 6 cut(s) 236, 1310, 1651, 2198, 2611, 2853
BspMI ACCTGC 2 cut(s) 569, 2262
BspPI GGATC 2 cut(s) 414, 2192
BspQI GCTCTTC 1 cut(s) 2300
BspT104I TTCGAA 1 cut(s) 839
BspT107I GGYRCC 1 cut(s) 1649
BspTI CTTAAG 1 cut(s) 605
BspTNI GGTCTC 1 cut(s) 212
BsrDI GCAATG 2 cut(s) 2148, 2872
BsrFI RCCGGY 3 cut(s) 287, 297, 1548
BsrGI TGTACA 4 cut(s) 989, 1816, 2355, 2649
BsrI ACTGG 5 cut(s) 894, 1171, 1375, 2368, 2392
BssAI RCCGGY 3 cut(s) 287, 297, 1548
BssECI CCNNGG 5 cut(s) 463, 1312, 1761, 1839, 2724
BssMI GATC 5 cut(s) 172, 406, 1906, 2184, 2644
BssNAI GTATAC 1 cut(s) 753
BssNI GRCGYC 2 cut(s) 201, 1546
BssT1I CCWWGG 3 cut(s) 1312, 1761, 1839
Bst1107I GTATAC 1 cut(s) 753
Bst2UI CCWGG 3 cut(s) 2201, 2525, 2731
Bst4CI ACNGT 8 cut(s) 329, 845, 872, 1799, 2095, 2355, 2673, 2839
Bst6I CTCTTC 5 cut(s) 116, 551, 1927, 2127, 2300
BstACI GRCGYC 2 cut(s) 201, 1546
BstAFI CTTAAG 1 cut(s) 605
BstAPI GCANNNNNTGC 1 cut(s) 2147
BstAUI TGTACA 4 cut(s) 989, 1816, 2355, 2649
BstBAI YACGTR 1 cut(s) 1646
BstBI TTCGAA 1 cut(s) 839
BstC8I GCNNGC 3 cut(s) 2148, 2165, 2659
BstDEI CTNAG 6 cut(s) 661, 1199, 1342, 1379, 1434, 2322
BstDSI CCRYGG 1 cut(s) 2724
BstEII GGTNACC 1 cut(s) 866
BstFNI CGCG 1 cut(s) 2661
BstHHI GCGC 2 cut(s) 2369, 2663
BstKTI GATC 5 cut(s) 175, 409, 1909, 2187, 2647
BstMAI GTCTC 4 cut(s) 212, 420, 878, 1176
BstMBI GATC 5 cut(s) 172, 406, 1906, 2184, 2644
BstMCI CGRYCG 2 cut(s) 298, 2803
BstMWI GCNNNNNNNGC 4 cut(s) 1691, 2086, 2147, 2307
BstNI CCWGG 3 cut(s) 2201, 2525, 2731
BstNSI RCATGY 4 cut(s) 1137, 1392, 1754, 2461
BstPI GGTNACC 1 cut(s) 866
BstSCI CCNGG 4 cut(s) 462, 2199, 2523, 2729
BstSLI GKGCMC 2 cut(s) 1174, 1654
BstUI CGCG 1 cut(s) 2661
BstV1I GCAGC 7 cut(s) 288, 1681, 1936, 1939, 2616, 2755, 2797
BstV2I GAAGAC 1 cut(s) 1663
BstX2I RGATCY 1 cut(s) 406
BstYI RGATCY 1 cut(s) 406
BstZ17I GTATAC 1 cut(s) 753
BstZI CGGCCG 1 cut(s) 2800
Bsu36I CCTNAGG 1 cut(s) 1434
BsuI GTATCC 2 cut(s) 1340, 1828
BsuRI GGCC 5 cut(s) 349, 1848, 2425, 2729, 2802
BtgI CCRYGG 1 cut(s) 2724
BtgZI GCGATG 1 cut(s) 2417
BtrI CACGTC 1 cut(s) 391
BtsI GCAGTG 1 cut(s) 190
BtsIMutI CAGTG 6 cut(s) 190, 2091, 2351, 2369, 2399, 2835
BveI ACCTGC 2 cut(s) 569, 2262
Cac8I GCNNGC 3 cut(s) 2148, 2165, 2659
CaiI CAGNNNCTG 1 cut(s) 1015
CciI TCATGA 1 cut(s) 1663
CfoI GCGC 2 cut(s) 2369, 2663
Cfr10I RCCGGY 3 cut(s) 287, 297, 1548
Cfr13I GGNCC 3 cut(s) 348, 564, 2851
CseI GACGC 1 cut(s) 1554
Csp6I GTAC 9 cut(s) 956, 990, 1067, 1580, 1817, 2172, 2356, 2403, 2650
CspAI ACCGGT 1 cut(s) 297
CspCI CAANNNNNGTGG 8 cut(s) 1018, 1053, 1066, 1101, 1478, 1513, 2532, 2567
CviQI GTAC 9 cut(s) 956, 990, 1067, 1580, 1817, 2172, 2356, 2403, 2650
DdeI CTNAG 6 cut(s) 661, 1199, 1342, 1379, 1434, 2322
DpnI GATC 5 cut(s) 174, 408, 1908, 2186, 2646
DpnII GATC 5 cut(s) 172, 406, 1906, 2184, 2644
DraIII CACNNNGTG 1 cut(s) 1649
DriI GACNNNNNGTC 1 cut(s) 2503
EaeI YGGCCR 2 cut(s) 1846, 2800
EagI CGGCCG 1 cut(s) 2800
Eam1104I CTCTTC 5 cut(s) 116, 551, 1927, 2127, 2300
Eam1105I GACNNNNNGTC 1 cut(s) 2503
EarI CTCTTC 5 cut(s) 116, 551, 1927, 2127, 2300
Ecl136II GAGCTC 2 cut(s) 963, 2657
EclXI CGGCCG 1 cut(s) 2800
Eco130I CCWWGG 3 cut(s) 1312, 1761, 1839
Eco24I GRGCYC 3 cut(s) 965, 2587, 2659
Eco31I GGTCTC 1 cut(s) 212
Eco47I GGWCC 2 cut(s) 564, 2851
Eco52I CGGCCG 1 cut(s) 2800
Eco53kI GAGCTC 2 cut(s) 963, 2657
Eco57I CTGAAG 4 cut(s) 395, 467, 1279, 1719
Eco72I CACGTG 1 cut(s) 1646
Eco81I CCTNAGG 1 cut(s) 1434
Eco88I CYCGRG 1 cut(s) 875
Eco91I GGTNACC 1 cut(s) 866
EcoICRI GAGCTC 2 cut(s) 963, 2657
EcoO109I RGGNCCY 1 cut(s) 2851
EcoO65I GGTNACC 1 cut(s) 866
EcoRI GAATTC 3 cut(s) 1046, 1916, 2569
EcoRII CCWGG 3 cut(s) 2199, 2523, 2729
EcoT14I CCWWGG 3 cut(s) 1312, 1761, 1839
EcoT22I ATGCAT 1 cut(s) 1736
EcoT38I GRGCYC 3 cut(s) 965, 2587, 2659
ErhI CCWWGG 3 cut(s) 1312, 1761, 1839
Esp3I CGTCTC 1 cut(s) 878
FalI AAGNNNNNCTT 2 cut(s) 1622, 1654
FaqI GGGAC 4 cut(s) 480, 994, 1495, 2837
FauI CCCGC 1 cut(s) 2484
FbaI TGATCA 2 cut(s) 172, 1906
FblI GTMKAC 1 cut(s) 752
Fnu4HI GCNGC 9 cut(s) 277, 292, 1695, 1950, 1953, 2630, 2769, 2786, 2800
FriOI GRGCYC 3 cut(s) 965, 2587, 2659
Fsp4HI GCNGC 9 cut(s) 277, 292, 1695, 1950, 1953, 2630, 2769, 2786, 2800
FspBI CTAG 6 cut(s) 33, 594, 630, 1035, 1071, 1691
GlaI GCGC 2 cut(s) 2368, 2662
GluI GCNGC 9 cut(s) 277, 292, 1695, 1950, 1953, 2630, 2769, 2786, 2800
GsaI CCCAGC 1 cut(s) 1410
GsuI CTGGAG 1 cut(s) 2183
HaeIII GGCC 5 cut(s) 349, 1848, 2425, 2729, 2802
HapII CCGG 9 cut(s) 288, 298, 318, 439, 463, 479, 1214, 1534, 1549
HgaI GACGC 1 cut(s) 1554
HhaI GCGC 2 cut(s) 2369, 2663
Hin1I GRCGYC 2 cut(s) 201, 1546
Hin6I GCGC 2 cut(s) 2367, 2661
HinP1I GCGC 2 cut(s) 2367, 2661
HindIII AAGCTT 1 cut(s) 608
HpaII CCGG 9 cut(s) 288, 298, 318, 439, 463, 479, 1214, 1534, 1549
Hpy166II GTNNAC 8 cut(s) 457, 753, 990, 1172, 1819, 2130, 2389, 2473
Hpy188III TCNNGA 7 cut(s) 170, 875, 877, 1051, 1323, 1664, 2283
Hpy8I GTNNAC 8 cut(s) 457, 753, 990, 1172, 1819, 2130, 2389, 2473
Hpy99I CGWCG 3 cut(s) 203, 206, 326
HpyAV CCTTC 8 cut(s) 31, 78, 989, 1085, 1102, 1694, 2323, 2450
HpyCH4III ACNGT 8 cut(s) 329, 845, 872, 1799, 2095, 2355, 2673, 2839
HpyCH4IV ACGT 4 cut(s) 201, 390, 1079, 1645
HpyF10VI GCNNNNNNNGC 4 cut(s) 1691, 2086, 2147, 2307
HpyF3I CTNAG 6 cut(s) 661, 1199, 1342, 1379, 1434, 2322
HpySE526I ACGT 4 cut(s) 201, 390, 1079, 1645
Hsp92I GRCGYC 2 cut(s) 201, 1546
HspAI GCGC 2 cut(s) 2367, 2661
Ksp22I TGATCA 2 cut(s) 172, 1906
Kzo9I GATC 5 cut(s) 172, 406, 1906, 2184, 2644
LguI GCTCTTC 1 cut(s) 2300
LmnI GCTCC 5 cut(s) 657, 1263, 2070, 2202, 2544
Lsp1109I GCAGC 7 cut(s) 288, 1681, 1936, 1939, 2616, 2755, 2797
LweI GCATC 3 cut(s) 697, 1002, 2473
MaeI CTAG 6 cut(s) 33, 594, 630, 1035, 1071, 1691
MaeII ACGT 4 cut(s) 201, 390, 1079, 1645
MaeIII GTNAC 7 cut(s) 581, 866, 1289, 1366, 2236, 2449, 2673
MalI GATC 5 cut(s) 174, 408, 1908, 2186, 2646
MboI GATC 5 cut(s) 172, 406, 1906, 2184, 2644
MflI RGATCY 1 cut(s) 406
MhlI GDGCHC 6 cut(s) 965, 1174, 1654, 2067, 2587, 2659
MlsI TGGCCA 1 cut(s) 1848
MluNI TGGCCA 1 cut(s) 1848
MlyI GAGTC 3 cut(s) 427, 976, 1166
MmeI TCCRAC 4 cut(s) 210, 928, 1053, 1159
Mox20I TGGCCA 1 cut(s) 1848
Mph1103I ATGCAT 1 cut(s) 1736
MscI TGGCCA 1 cut(s) 1848
MseI TTAA 7 cut(s) 606, 612, 1779, 1806, 2205, 2295, 2348
MslI CAYNNNNRTG 3 cut(s) 744, 1469, 2640
Msp20I TGGCCA 1 cut(s) 1848
MspCI CTTAAG 1 cut(s) 605
MspI CCGG 9 cut(s) 288, 298, 318, 439, 463, 479, 1214, 1534, 1549
MspR9I CCNGG 4 cut(s) 464, 2201, 2525, 2731
MvaI CCWGG 3 cut(s) 2201, 2525, 2731
MvnI CGCG 1 cut(s) 2661
MwoI GCNNNNNNNGC 4 cut(s) 1691, 2086, 2147, 2307
NciI CCSGG 1 cut(s) 464
NdeII GATC 5 cut(s) 172, 406, 1906, 2184, 2644
NlaIV GGNNCC 6 cut(s) 236, 1310, 1651, 2198, 2611, 2853
NmuCI GTSAC 2 cut(s) 1366, 2449
NsiI ATGCAT 1 cut(s) 1736
NspI RCATGY 4 cut(s) 1137, 1392, 1754, 2461
NspV TTCGAA 1 cut(s) 839
PaeR7I CTCGAG 1 cut(s) 875
PagI TCATGA 1 cut(s) 1663
PaqCI CACCTGC 1 cut(s) 2262
PciI ACATGT 2 cut(s) 1388, 2457
PciSI GCTCTTC 1 cut(s) 2300
PflFI GACNNNGTC 1 cut(s) 2275
PflMI CCANNNNNTGG 1 cut(s) 2730
PfoI TCCNGGA 1 cut(s) 2523
PinAI ACCGGT 1 cut(s) 297
PkrI GCNGC 9 cut(s) 278, 293, 1696, 1951, 1954, 2631, 2770, 2787, 2801
PleI GAGTC 3 cut(s) 427, 976, 1165
PmaCI CACGTG 1 cut(s) 1646
PmlI CACGTG 1 cut(s) 1646
PpsI GAGTC 3 cut(s) 427, 976, 1165
Ppu21I YACGTR 1 cut(s) 1646
PpuMI RGGWCCY 1 cut(s) 2851
PscI ACATGT 2 cut(s) 1388, 2457
Psp124BI GAGCTC 2 cut(s) 965, 2659
Psp5II RGGWCCY 1 cut(s) 2851
Psp6I CCWGG 3 cut(s) 2199, 2523, 2729
PspCI CACGTG 1 cut(s) 1646
PspEI GGTNACC 1 cut(s) 866
PspFI CCCAGC 1 cut(s) 1406
PspGI CCWGG 3 cut(s) 2199, 2523, 2729
PspN4I GGNNCC 6 cut(s) 236, 1310, 1651, 2198, 2611, 2853
PspPI GGNCC 3 cut(s) 348, 564, 2851
PspPPI RGGWCCY 1 cut(s) 2851
PstNI CAGNNNCTG 1 cut(s) 1015
PsuI RGATCY 1 cut(s) 406
PsyI GACNNNGTC 1 cut(s) 2275
RsaI GTAC 9 cut(s) 957, 991, 1068, 1581, 1818, 2173, 2357, 2404, 2651
RsaNI GTAC 9 cut(s) 956, 990, 1067, 1580, 1817, 2172, 2356, 2403, 2650
RseI CAYNNNNRTG 3 cut(s) 744, 1469, 2640
SacI GAGCTC 2 cut(s) 965, 2659
SapI GCTCTTC 1 cut(s) 2300
SaqAI TTAA 7 cut(s) 606, 612, 1779, 1806, 2205, 2295, 2348
SatI GCNGC 9 cut(s) 277, 292, 1695, 1950, 1953, 2630, 2769, 2786, 2800
Sau3AI GATC 5 cut(s) 172, 406, 1906, 2184, 2644
Sau96I GGNCC 3 cut(s) 348, 564, 2851
ScaI AGTACT 2 cut(s) 2173, 2404
SchI GAGTC 3 cut(s) 427, 976, 1166
ScrFI CCNGG 4 cut(s) 464, 2201, 2525, 2731
SduI GDGCHC 6 cut(s) 965, 1174, 1654, 2067, 2587, 2659
SfaNI GCATC 3 cut(s) 697, 1002, 2473
Sfr274I CTCGAG 1 cut(s) 875
SfuI TTCGAA 1 cut(s) 839
SgrAI CRCCGGYG 1 cut(s) 287
SinI GGWCC 2 cut(s) 564, 2851
SlaI CTCGAG 1 cut(s) 875
SmiMI CAYNNNNRTG 3 cut(s) 744, 1469, 2640
SmlI CTYRAG 5 cut(s) 605, 875, 1051, 1204, 2501
SmoI CTYRAG 5 cut(s) 605, 875, 1051, 1204, 2501
SsiI CCGC 4 cut(s) 291, 1088, 2477, 2799
SspI AATATT 3 cut(s) 1361, 2550, 2823
SspMI CTAG 6 cut(s) 33, 594, 630, 1035, 1071, 1691
SstI GAGCTC 2 cut(s) 965, 2659
StyD4I CCNGG 4 cut(s) 462, 2199, 2523, 2729
StyI CCWWGG 3 cut(s) 1312, 1761, 1839
TaaI ACNGT 8 cut(s) 329, 845, 872, 1799, 2095, 2355, 2673, 2839
TaiI ACGT 4 cut(s) 204, 393, 1082, 1648
TaqI TCGA 6 cut(s) 431, 588, 839, 876, 1775, 1869
TatI WGTACW 6 cut(s) 989, 1816, 2171, 2355, 2402, 2649
TauI GCSGC 2 cut(s) 294, 2802
Tru1I TTAA 7 cut(s) 606, 612, 1779, 1806, 2205, 2295, 2348
Tru9I TTAA 7 cut(s) 606, 612, 1779, 1806, 2205, 2295, 2348
TscAI CASTG 6 cut(s) 197, 2098, 2358, 2376, 2399, 2842
TseFI GTSAC 2 cut(s) 1366, 2449
TseI GCWGC 7 cut(s) 276, 1694, 1949, 1952, 2629, 2768, 2785
Tsp45I GTSAC 2 cut(s) 1366, 2449
TspGWI ACGGA 2 cut(s) 241, 1737
TspRI CASTG 6 cut(s) 197, 2098, 2358, 2376, 2399, 2842
Tth111I GACNNNGTC 1 cut(s) 2275
Van91I CCANNNNNTGG 1 cut(s) 2730
Vha464I CTTAAG 1 cut(s) 605
VneI GTGCAC 1 cut(s) 1170
VpaK11BI GGWCC 2 cut(s) 564, 2851
XapI RAATTY 7 cut(s) 826, 1046, 1147, 1257, 1457, 1916, 2569
XceI RCATGY 4 cut(s) 1137, 1392, 1754, 2461
XhoI CTCGAG 1 cut(s) 875
XmiI GTMKAC 1 cut(s) 752
XspI CTAG 6 cut(s) 33, 594, 630, 1035, 1071, 1691
ZraI GACGTC 1 cut(s) 202
ZrmI AGTACT 2 cut(s) 2173, 2404
Zsp2I ATGCAT 1 cut(s) 1736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.