RLG00000033506

Protein of unknown function (DUF2921)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000420
Physical Location & Seq
Reverse (-)
28240013 .. 28243343
3331 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033506

Sequence Viewer

Length: 2472 bp
ATGACTATCTTCAGAAAGCCAAAGCTGGTCATATTCTTCTTCTGTGTTTTCATCACCCTGTCTTTGAACTCAATCTCATCCTTTGCTCTACCTAAGAAAATCGCTTATGCTGATAATTGTGCTTCAGTTGTTCCCGAGTCAACCCCGAAAGCGGACAGCAGAGATCATCGCCATGAGTATCTCCATACAGCTTACTACTACACTGGTGGTGACACTGGCAATGGTGGTCTTAGTCCATATGGTTATTATTATCAGCTCCAAAACTCAGTTGAATTCCACGTCTGGAGTTTCGAAGAAACTGCTGTACAAGGCTTGTTCAAGCTCCGAGCAAATCTTCAATTTCCAAAGGCCAGAACATATTACTACGTGGGAAACTCCACAAGTAGTAGTAGCAGCTCCAGGTCTCAATCAATTCATGCACATCCAAGGTGGTCACTCAACTTTAGACTCAACGGGTTCTGGTCAGAATCTTCAGGGAAGCTTTGTATGGTTGGATATGGTAGTTACTCGAAGAGAGGTACTTCACTCAGTTACCCTGCTATCCTTAAGCTCTATAATCTCATCAATTCCACTAGTATTACAAGTTTGATTACTGGAACCATAGAGTCTTTGATCCCGAGTTCTGATATTGTCAAGGATCCAAGATATTTTGATCCAACCTCTATTTTGATGCTTCCTCGGATGAACTACCAGTACACTTTGGTTTCAAATAACTCTAATGAAGACCCCTCTTTTGGTGACACTGATGATCATGCTCCCTCGAGTTCTTTGAAATTGGAGAACTTTTGTTCACAACTCTCAACAGTAGTACTAAAGAATGAGTTTGATCTCAAATATTCAAGCAAATGTGTTTCTGCAAGGAATTGTACTCCATTTGCTGGGGTAGATCCTTTGCCTCGACTCGTGTCGTTTAAGGATATTGATTGTAAGGAGAGAAATAGAAGATTGAGAGTTCTGGTGGAGTTCTCAGACAGCAGCAACAGATGGTTTCAAAGGCCTTTCAATCCCAACACAACTCTGGTTGCAGAAGGCTCATGGGATGCAAAGAAAAATCAACTGAAATTTGTTGCTTGTCATTTCTTAGAGGCAGCAACAGATTCTTTCAATAATTCTCATGTGGGTGACTGTTCAACAAGATTGAGCTTGAGATTTCCTGCAACATGGACAATCGGAAACACTAGTACCGTTGTGGGGCATATTTGGAGCAACAAATCCAAGACGGAGTCTGGTTACTTTGAAAAGATCAGATTCGAAACTTGTCAGCGTGAGGCTGGAAGGATTACAGTTCCAGGCACCAAATATGAGTACACAAAACTTGACCAAGTAAACAAGTTGTGCCCAAGAAAGAAGACTGCTGCTAATGGTCACAAAACCAATGTGTACCCAAGTCCAATTTCATATGACATGAGATTCGATATGTCAGCTAGAAGTTCTAAGGGACATTTTGAAGCATGGGGAAGTTCTGAACCTGTCTCTGTCGGCGATAGGTTGTACGAGGAGCCTTCTTCTTCAAGGCAATATAGTGGCTCAGCTGCAATCAATGAAGATGAAAATTACTCTCTAGGGATAATTTCCAGCTACAACAACAGCAACCCGTCCAATATTAGCTACAGAATAAGCATTCAATTGCTGGGGCGATATGCTGGAAAGTTGGGCAACACAACGCAAATGACGGAAATGCATATCTCTGCTGAAGGGATATATGATACAGAAGGAAGCCTGTGCATGGTGGGCTGCCGAAATGTTGGCTTAAACAGTGATCACCAGACCACAGAAGATTCTGTAGATTGTAAGATCCTTGTCAATTTCCAGTTCCCTCCAGACAATCAACACAGTGAAAATAAAGGTCATATCAAGGTGAGGATTGAAAGCACACGAGAAAAGTCTGATCCTCTTCATTTCGAGCGATTGGATTTGTCATCAGTTGCTGGTTACATAAGTGAAGCAGAACGATCCATCTGGAGGATGGACATGGAGATCATCTTGGTTCTTGTATCTACCACACTAGCATGTGTATTTGTGGCATTACAACAGTTTCATGTGAAAAAACATCCTGATGTGCTTCCCTCGATTTCCATCCTGATGCTACTGATTCTAACCGTTGGCTACATGATACCTCTTTTGCTAAACTTTGATGCCATGTTCACTGACAACACCAACCGCCAATATGTGTCACTGGGAAGTGGTGGATGGCTTGCAGTTCACGAGGTAATTGTAAGGGTTATAACAATGGTAGCTTTCTTGTTGCAATTCCGTCTTCTGCAGCAGACATGGTCGGCAAGATCAGCAAATGGGAAGCAAAAGGAGCTTTGGGATGTGGAGAAGAAGGTTTTACCGCTGTATCCAATTGGGTATTTAGTTGCTTGGGCAGTGCTTCCCATTTCTGTGGACTTGAAATCTTATGCTGTTCGACAGATTTGGAAGGGCAGATCAGAGCTCAACAACGCCCATGTCTCCACCAGTGTAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
DUF2921

Protein Analysis

824

Amino Acids

92.73

Weight (kDa)

8.65

Isoelectric Point (pI)

39.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2921_N PF25333 36 - 220 1.3e-18 DUF2921 lipocalin-like domain
DUF2921_N PF25333 245 - 415 6.9e-31 DUF2921 lipocalin-like domain
DUF2921_N PF25333 442 - 638 1.1e-30 DUF2921 lipocalin-like domain
DUF2921 PF11145 649 - 792 2.8e-30 Transmembrane E3 ligase/DUF2921 transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21700
fragaria_vesca FvH4_1g10360 FvH4_1g10760 FvH4_2g30370 FvH4_3g09240 FvH4_3g20050 FvH4_3g20280 FvH4_3g20290 FvH4_3g20291 FvH4_3g20340 FvH4_3g20350 FvH4_3g20351 FvH4_3g20380 FvH4_3g20390 FvH4_5g13190 FvH4_6g20070 FvH4_6g42890
malus_domestica MD03G1227000.v1.1 MD10G1294900.v1.1 MD11G1246300.v1.1 MD11G1246400.v1.1 MD11G1246500.v1.1 MD11G1246600.v1.1
prunus_persica Prupe.4G047300_v2.0.a1 Prupe.4G181700_v2.0.a1 Prupe.4G181900_v2.0.a1 Prupe.4G182000_v2.0.a1 Prupe.4G182200_v2.0.a1
pyrus_communis pycom03g17470 pycom10g24670 pycom11g21640
rosa_chinensis RchiOBHm_Chr5g0008381 RchiOBHm_Chr5g0008391 RchiOBHm_Chr5g0033881 RchiOBHm_Chr5g0034171 RchiOBHm_Chr5g0034181 RchiOBHm_Chr5g0034191 RchiOBHm_Chr5g0034201 RchiOBHm_Chr5g0034211 RchiOBHm_Chr5g0034221 RchiOBHm_Chr5g0034231 RchiOBHm_Chr5g0034271 RchiOBHm_Chr5g0034281
rosa_laevigata RLG00000031566 RLG00000031567 RLG00000033506 RLG00000033529 RLG00000033530 RLG00000033531
rosa_multiflora Rmu_co8445509.1_g000001 Rmu_co8470615.1_g000001 Rmu_sc0008805.1_g000003 Rmu_sc0008805.1_g000004 Rmu_ssc0000128.1_g000029 Rmu_ssc0000128.1_g000031 Rmu_ssc0000128.1_g000032 Rmu_ssc0000388.1_g000062 Rmu_ssc0000388.1_g000063
rosa_roxburghii Rroxscaffold_1G00046010 Rroxscaffold_1G00046020 Rroxscaffold_1G00046030 Rroxscaffold_1G00046040 Rroxscaffold_1G00046060 Rroxscaffold_1G00046070 Rroxscaffold_1G00046080 Rroxscaffold_1G00046090
rosa_rugosa Rorug04G0435500 Rorug05G0142900 Rorug05G0144600 Rorug05G0144600 Rorug05G0144700 Rorug05G0144800
rosa_samantha Rh5AG233800 Rh5AG235900 Rh5AG236000 Rh5AG236100 Rh5AG236200 Rh5AG236300 Rh5AG236500 Rh5BG061200 Rh5BG061300 Rh5BG234300 Rh5BG236600 Rh5BG236800 Rh5CG071200 Rh5DG060000 Rh5DG060100 Rh5DG241300 Rh5DG243700 Rh5DG244000 Rh5DG244100
rosa_wichuraiana Rw3G018950 Rw5G005680 Rw5G021330 Rw5G021340 Rw5G021350 Rw5G021550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2225
AccB1I GGYRCC 1 cut(s) 1292
AccB7I CCANNNNNTGG 1 cut(s) 878
AciI CCGC 3 cut(s) 152, 2161, 2336
AclWI GGATC 8 cut(s) 607, 632, 645, 647, 881, 1789, 1883, 1947
AcsI RAATTY 2 cut(s) 272, 1061
AcuI CTGAAG 3 cut(s) 108, 456, 1713
AfaI GTAC 9 cut(s) 306, 520, 695, 810, 868, 1183, 1307, 1382, 1493
AfiI CCNNNNNNNGG 6 cut(s) 151, 734, 878, 1191, 1726, 1962
AflII CTTAAG 1 cut(s) 545
AhlI ACTAGT 2 cut(s) 572, 1178
AjiI CACGTC 1 cut(s) 280
AjnI CCWGG 2 cut(s) 398, 1288
AjuI GAANNNNNNNTTGG 2 cut(s) 1378, 1410
Alw21I GWGCWC 1 cut(s) 2439
Alw26I GTCTC 3 cut(s) 408, 1477, 2458
AlwI GGATC 8 cut(s) 607, 632, 645, 647, 881, 1789, 1883, 1947
AlwNI CAGNNNCTG 1 cut(s) 1928
Ama87I CYCGRG 3 cut(s) 134, 616, 760
AoxI GGCC 2 cut(s) 348, 995
ApeKI GCWGC 7 cut(s) 393, 975, 1088, 1355, 1532, 1734, 2263
ApoI RAATTY 2 cut(s) 272, 1061
ArsI GACNNNNNNTTYG 4 cut(s) 716, 748, 1394, 1426
AsuHPI GGTGA 6 cut(s) 46, 221, 749, 1133, 1754, 1870
AsuII TTCGAA 2 cut(s) 291, 1251
AvaI CYCGRG 3 cut(s) 134, 616, 760
BaeGI GKGCMC 1 cut(s) 1340
BaeI ACNNNNGTAYC 4 cut(s) 1165, 1198, 2324, 2357
BamHI GGATCC 1 cut(s) 637
BanI GGYRCC 1 cut(s) 1292
BanII GRGCYC 1 cut(s) 2439
BauI CACGAG 3 cut(s) 902, 1875, 2204
BbsI GAAGAC 3 cut(s) 729, 1355, 2249
Bbv12I GWGCWC 1 cut(s) 2439
BbvI GCAGC 7 cut(s) 405, 987, 1100, 1342, 1519, 1721, 2275
BccI CCATC 5 cut(s) 978, 1960, 1964, 2084, 2184
BcgI CGANNNNNNTGC 2 cut(s) 281, 315
BciT130I CCWGG 2 cut(s) 400, 1290
BciVI GTATCC 1 cut(s) 2352
BclI TGATCA 2 cut(s) 748, 1759
BcoDI GTCTC 3 cut(s) 408, 1477, 2458
BcuI ACTAGT 2 cut(s) 572, 1178
BfaI CTAG 5 cut(s) 573, 1179, 1425, 1562, 2006
BfmI CTRYAG 3 cut(s) 1609, 1782, 2261
BfrI CTTAAG 1 cut(s) 545
BfuI GTATCC 1 cut(s) 2352
BisI GCNGC 7 cut(s) 394, 976, 1089, 1356, 1533, 1735, 2264
BlpI GCTNAGC 1 cut(s) 1528
BlsI GCNGC 7 cut(s) 395, 977, 1090, 1357, 1534, 1736, 2265
BmcAI AGTACT 1 cut(s) 810
Bme1390I CCNGG 2 cut(s) 400, 1290
BmeT110I CYCGRG 3 cut(s) 134, 616, 760
BmgBI CACGTC 1 cut(s) 280
BmiI GGNNCC 4 cut(s) 598, 639, 1294, 1500
BmrFI CCNGG 2 cut(s) 400, 1290
BmrI ACTGGG 1 cut(s) 2186
BmsI GCATC 4 cut(s) 660, 1030, 2073, 2125
BmuI ACTGGG 1 cut(s) 2186
BoxI GACNNNNGTC 1 cut(s) 904
BpiI GAAGAC 3 cut(s) 729, 1355, 2249
BpmI CTGGAG 4 cut(s) 304, 382, 1803, 1981
Bpu1102I GCTNAGC 1 cut(s) 1528
Bpu14I TTCGAA 2 cut(s) 291, 1251
BpuEI CTTGAG 1 cut(s) 1165
BsaAI YACGTR 1 cut(s) 367
BsaBI GATNNNNATC 1 cut(s) 2075
BsaI GGTCTC 1 cut(s) 408
BsaJI CCNNGG 2 cut(s) 425, 677
Bsc4I CCNNNNNNNGG 6 cut(s) 151, 734, 878, 1191, 1726, 1962
Bse1I ACTGG 7 cut(s) 208, 220, 598, 691, 1810, 2181, 2460
Bse3DI GCAATG 1 cut(s) 226
Bse8I GATNNNNATC 1 cut(s) 2075
BseBI CCWGG 2 cut(s) 400, 1290
BseDI CCNNGG 2 cut(s) 425, 677
BseGI GGATG 9 cut(s) 77, 421, 687, 1045, 1971, 2050, 2076, 2195, 2320
BseJI GATNNNNATC 1 cut(s) 2075
BseLI CCNNNNNNNGG 6 cut(s) 151, 734, 878, 1191, 1726, 1962
BseMI GCAATG 1 cut(s) 226
BseMII CTCAG 4 cut(s) 279, 541, 981, 1542
BseNI ACTGG 7 cut(s) 208, 220, 598, 691, 1810, 2181, 2460
BseRI GAGGAG 1 cut(s) 1511
BseSI GKGCMC 1 cut(s) 1340
BseXI GCAGC 7 cut(s) 405, 987, 1100, 1342, 1519, 1721, 2275
BseYI CCCAGC 2 cut(s) 878, 1630
BshFI GGCC 2 cut(s) 350, 997
BshNI GGYRCC 1 cut(s) 1292
BsiHKAI GWGCWC 1 cut(s) 2439
BsiHKCI CYCGRG 3 cut(s) 134, 616, 760
BslFI GGGAC 1 cut(s) 1452
BslI CCNNNNNNNGG 6 cut(s) 151, 734, 878, 1191, 1726, 1962
BsmAI GTCTC 3 cut(s) 408, 1477, 2458
BsmFI GGGAC 1 cut(s) 1452
BsmI GAATGC 1 cut(s) 1620
BsnI GGCC 2 cut(s) 350, 997
Bso31I GGTCTC 1 cut(s) 408
BsoBI CYCGRG 3 cut(s) 134, 616, 760
Bsp119I TTCGAA 2 cut(s) 291, 1251
Bsp1286I GDGCHC 2 cut(s) 1340, 2439
Bsp1407I TGTACA 1 cut(s) 304
Bsp1720I GCTNAGC 1 cut(s) 1528
BspACI CCGC 3 cut(s) 152, 2161, 2336
BspANI GGCC 2 cut(s) 350, 997
BspCNI CTCAG 4 cut(s) 278, 540, 980, 1541
BspLI GGNNCC 4 cut(s) 598, 639, 1294, 1500
BspMAI CTGCAG 1 cut(s) 2265
BspPI GGATC 8 cut(s) 607, 632, 645, 647, 881, 1789, 1883, 1947
BspT104I TTCGAA 2 cut(s) 291, 1251
BspT107I GGYRCC 1 cut(s) 1292
BspTI CTTAAG 1 cut(s) 545
BspTNI GGTCTC 1 cut(s) 408
BsrDI GCAATG 1 cut(s) 226
BsrGI TGTACA 1 cut(s) 304
BsrI ACTGG 7 cut(s) 208, 220, 598, 691, 1810, 2181, 2460
BssECI CCNNGG 2 cut(s) 425, 677
BssSI CACGAG 3 cut(s) 902, 1875, 2204
BssT1I CCWWGG 1 cut(s) 425
Bst2BI CACGAG 3 cut(s) 902, 1875, 2204
Bst2UI CCWGG 2 cut(s) 400, 1290
Bst4CI ACNGT 8 cut(s) 805, 1127, 1186, 1285, 1757, 1835, 2034, 2101
Bst6I CTCTTC 2 cut(s) 506, 1899
BstAFI CTTAAG 1 cut(s) 545
BstAUI TGTACA 1 cut(s) 304
BstBAI YACGTR 1 cut(s) 367
BstBI TTCGAA 2 cut(s) 291, 1251
BstC8I GCNNGC 1 cut(s) 2196
BstDEI CTNAG 8 cut(s) 93, 230, 265, 527, 967, 1081, 1434, 1528
BstF5I GGATG 9 cut(s) 77, 421, 687, 1045, 1971, 2050, 2076, 2195, 2320
BstMAI GTCTC 3 cut(s) 408, 1477, 2458
BstMWI GCNNNNNNNGC 3 cut(s) 1731, 2285, 2305
BstNI CCWGG 2 cut(s) 400, 1290
BstNSI RCATGY 1 cut(s) 2013
BstPAI GACNNNNGTC 1 cut(s) 904
BstSCI CCNGG 2 cut(s) 398, 1288
BstSFI CTRYAG 3 cut(s) 1609, 1782, 2261
BstSLI GKGCMC 1 cut(s) 1340
BstV1I GCAGC 7 cut(s) 405, 987, 1100, 1342, 1519, 1721, 2275
BstV2I GAAGAC 3 cut(s) 729, 1355, 2249
BstX2I RGATCY 3 cut(s) 637, 886, 1794
BstXI CCANNNNNNTGG 1 cut(s) 2386
BstYI RGATCY 3 cut(s) 637, 886, 1794
BsuI GTATCC 1 cut(s) 2352
BsuRI GGCC 2 cut(s) 350, 997
BtgZI GCGATG 1 cut(s) 152
BtrI CACGTC 1 cut(s) 280
BtsCI GGATG 9 cut(s) 77, 421, 687, 1045, 1971, 2050, 2076, 2195, 2320
BtsI GCAGTG 1 cut(s) 2376
BtsIMutI CAGTG 9 cut(s) 201, 213, 741, 1762, 1840, 2145, 2174, 2376, 2467
Cac8I GCNNGC 1 cut(s) 2196
CaiI CAGNNNCTG 1 cut(s) 1928
Csp6I GTAC 9 cut(s) 305, 519, 694, 809, 867, 1182, 1306, 1381, 1492
CviQI GTAC 9 cut(s) 305, 519, 694, 809, 867, 1182, 1306, 1381, 1492
DdeI CTNAG 8 cut(s) 93, 230, 265, 527, 967, 1081, 1434, 1528
Eam1104I CTCTTC 2 cut(s) 506, 1899
EarI CTCTTC 2 cut(s) 506, 1899
Ecl136II GAGCTC 1 cut(s) 2437
Eco130I CCWWGG 1 cut(s) 425
Eco147I AGGCCT 1 cut(s) 997
Eco24I GRGCYC 1 cut(s) 2439
Eco31I GGTCTC 1 cut(s) 408
Eco53kI GAGCTC 1 cut(s) 2437
Eco57I CTGAAG 3 cut(s) 108, 456, 1713
Eco88I CYCGRG 3 cut(s) 134, 616, 760
EcoICRI GAGCTC 1 cut(s) 2437
EcoRI GAATTC 1 cut(s) 272
EcoRII CCWGG 2 cut(s) 398, 1288
EcoT14I CCWWGG 1 cut(s) 425
EcoT22I ATGCAT 1 cut(s) 1683
EcoT38I GRGCYC 1 cut(s) 2439
ErhI CCWWGG 1 cut(s) 425
FaqI GGGAC 1 cut(s) 1452
FauNDI CATATG 2 cut(s) 238, 1399
FbaI TGATCA 2 cut(s) 748, 1759
Fnu4HI GCNGC 7 cut(s) 394, 976, 1089, 1356, 1533, 1735, 2264
FokI GGATG 9 cut(s) 64, 408, 694, 1052, 1978, 2037, 2063, 2202, 2327
FriOI GRGCYC 1 cut(s) 2439
Fsp4HI GCNGC 7 cut(s) 394, 976, 1089, 1356, 1533, 1735, 2264
FspBI CTAG 5 cut(s) 573, 1179, 1425, 1562, 2006
GluI GCNGC 7 cut(s) 394, 976, 1089, 1356, 1533, 1735, 2264
GsaI CCCAGC 2 cut(s) 882, 1634
GsuI CTGGAG 4 cut(s) 304, 382, 1803, 1981
HaeIII GGCC 2 cut(s) 350, 997
HincII GTYRAC 1 cut(s) 141
HindII GTYRAC 1 cut(s) 141
HindIII AAGCTT 1 cut(s) 479
HphI GGTGA 6 cut(s) 46, 221, 749, 1133, 1754, 1870
Hpy166II GTNNAC 9 cut(s) 141, 696, 791, 1308, 1327, 1381, 2145, 2203, 2389
Hpy188III TCNNGA 8 cut(s) 134, 283, 616, 1820, 1960, 2054, 2080, 2204
Hpy8I GTNNAC 9 cut(s) 141, 696, 791, 1308, 1327, 1381, 2145, 2203, 2389
HpyAV CCTTC 7 cut(s) 1022, 1269, 1512, 1688, 1706, 2320, 2416
HpyCH4III ACNGT 8 cut(s) 805, 1127, 1186, 1285, 1757, 1835, 2034, 2101
HpyCH4IV ACGT 2 cut(s) 279, 366
HpyF10VI GCNNNNNNNGC 3 cut(s) 1731, 2285, 2305
HpyF3I CTNAG 8 cut(s) 93, 230, 265, 527, 967, 1081, 1434, 1528
HpySE526I ACGT 2 cut(s) 279, 366
Ksp22I TGATCA 2 cut(s) 748, 1759
LmnI GCTCC 7 cut(s) 261, 327, 401, 760, 1203, 1498, 2305
Lsp1109I GCAGC 7 cut(s) 405, 987, 1100, 1342, 1519, 1721, 2275
LweI GCATC 4 cut(s) 660, 1030, 2073, 2125
MaeI CTAG 5 cut(s) 573, 1179, 1425, 1562, 2006
MaeII ACGT 2 cut(s) 279, 366
MfeI CAATTG 2 cut(s) 1625, 2346
MflI RGATCY 3 cut(s) 637, 886, 1794
MhlI GDGCHC 2 cut(s) 1340, 2439
MlyI GAGTC 5 cut(s) 146, 441, 614, 894, 1232
MmeI TCCRAC 2 cut(s) 472, 680
Mph1103I ATGCAT 1 cut(s) 1683
MseI TTAA 3 cut(s) 546, 912, 1751
MslI CAYNNNNRTG 6 cut(s) 171, 1119, 2008, 2055, 2081, 2384
MspA1I CMGCKG 2 cut(s) 1532, 2338
MspCI CTTAAG 1 cut(s) 545
MspR9I CCNGG 2 cut(s) 400, 1290
MunI CAATTG 2 cut(s) 1625, 2346
Mva1269I GAATGC 1 cut(s) 1620
MvaI CCWGG 2 cut(s) 400, 1290
MwoI GCNNNNNNNGC 3 cut(s) 1731, 2285, 2305
NdeI CATATG 2 cut(s) 238, 1399
NlaIV GGNNCC 4 cut(s) 598, 639, 1294, 1500
NmuCI GTSAC 6 cut(s) 209, 432, 737, 1121, 1364, 2172
NsiI ATGCAT 1 cut(s) 1683
NspI RCATGY 1 cut(s) 2013
NspV TTCGAA 2 cut(s) 291, 1251
PaeR7I CTCGAG 1 cut(s) 760
PceI AGGCCT 1 cut(s) 997
PctI GAATGC 1 cut(s) 1620
PfeI GAWTC 6 cut(s) 467, 1097, 1248, 1410, 1778, 2092
PflFI GACNNNGTC 2 cut(s) 1222, 2272
PflMI CCANNNNNTGG 1 cut(s) 878
PkrI GCNGC 7 cut(s) 395, 977, 1090, 1357, 1534, 1736, 2265
PleI GAGTC 5 cut(s) 145, 441, 613, 894, 1231
PpsI GAGTC 5 cut(s) 145, 441, 613, 894, 1231
Ppu21I YACGTR 1 cut(s) 367
PshAI GACNNNNGTC 1 cut(s) 904
PsiI TTATAA 1 cut(s) 2225
Psp124BI GAGCTC 1 cut(s) 2439
Psp6I CCWGG 2 cut(s) 398, 1288
PspFI CCCAGC 2 cut(s) 878, 1630
PspGI CCWGG 2 cut(s) 398, 1288
PspN4I GGNNCC 4 cut(s) 598, 639, 1294, 1500
PspXI VCTCGAGB 1 cut(s) 760
PsrI GAACNNNNNNTAC 2 cut(s) 677, 709
PstI CTGCAG 1 cut(s) 2265
PstNI CAGNNNCTG 1 cut(s) 1928
PsuI RGATCY 3 cut(s) 637, 886, 1794
PsyI GACNNNGTC 2 cut(s) 1222, 2272
PvuII CAGCTG 1 cut(s) 1532
RsaI GTAC 9 cut(s) 306, 520, 695, 810, 868, 1183, 1307, 1382, 1493
RsaNI GTAC 9 cut(s) 305, 519, 694, 809, 867, 1182, 1306, 1381, 1492
RseI CAYNNNNRTG 6 cut(s) 171, 1119, 2008, 2055, 2081, 2384
SacI GAGCTC 1 cut(s) 2439
SaqAI TTAA 3 cut(s) 546, 912, 1751
SatI GCNGC 7 cut(s) 394, 976, 1089, 1356, 1533, 1735, 2264
ScaI AGTACT 1 cut(s) 810
SchI GAGTC 5 cut(s) 146, 441, 614, 894, 1232
ScrFI CCNGG 2 cut(s) 400, 1290
SduI GDGCHC 2 cut(s) 1340, 2439
SfaNI GCATC 4 cut(s) 660, 1030, 2073, 2125
SfcI CTRYAG 3 cut(s) 1609, 1782, 2261
Sfr274I CTCGAG 1 cut(s) 760
SfuI TTCGAA 2 cut(s) 291, 1251
SlaI CTCGAG 1 cut(s) 760
SmiMI CAYNNNNRTG 6 cut(s) 171, 1119, 2008, 2055, 2081, 2384
SmlI CTYRAG 3 cut(s) 545, 760, 1144
SmoI CTYRAG 3 cut(s) 545, 760, 1144
SpeI ACTAGT 2 cut(s) 572, 1178
SseBI AGGCCT 1 cut(s) 997
SsiI CCGC 3 cut(s) 152, 2161, 2336
SspI AATATT 2 cut(s) 836, 1603
SspMI CTAG 5 cut(s) 573, 1179, 1425, 1562, 2006
SstI GAGCTC 1 cut(s) 2439
StuI AGGCCT 1 cut(s) 997
StyD4I CCNGG 2 cut(s) 398, 1288
StyI CCWWGG 1 cut(s) 425
TaaI ACNGT 8 cut(s) 805, 1127, 1186, 1285, 1757, 1835, 2034, 2101
TaiI ACGT 2 cut(s) 282, 369
TaqI TCGA 9 cut(s) 291, 509, 761, 898, 1251, 1413, 1902, 2069, 2410
TatI WGTACW 5 cut(s) 304, 693, 808, 866, 1305
TfiI GAWTC 6 cut(s) 467, 1097, 1248, 1410, 1778, 2092
Tru1I TTAA 3 cut(s) 546, 912, 1751
Tru9I TTAA 3 cut(s) 546, 912, 1751
TscAI CASTG 9 cut(s) 208, 220, 748, 1762, 1840, 2152, 2181, 2376, 2467
TseFI GTSAC 6 cut(s) 209, 432, 737, 1121, 1364, 2172
TseI GCWGC 7 cut(s) 393, 975, 1088, 1355, 1532, 1734, 2263
Tsp45I GTSAC 6 cut(s) 209, 432, 737, 1121, 1364, 2172
TspDTI ATGAA 9 cut(s) 40, 404, 698, 735, 1386, 1557, 1563, 1886, 2027
TspGWI ACGGA 3 cut(s) 1235, 1688, 2243
TspRI CASTG 9 cut(s) 208, 220, 748, 1762, 1840, 2152, 2181, 2376, 2467
Tth111I GACNNNGTC 2 cut(s) 1222, 2272
Van91I CCANNNNNTGG 1 cut(s) 878
Vha464I CTTAAG 1 cut(s) 545
XapI RAATTY 2 cut(s) 272, 1061
XceI RCATGY 1 cut(s) 2013
XcmI CCANNNNNNNNNTGG 2 cut(s) 1015, 1963
XhoI CTCGAG 1 cut(s) 760
XspI CTAG 5 cut(s) 573, 1179, 1425, 1562, 2006
ZrmI AGTACT 1 cut(s) 810
Zsp2I ATGCAT 1 cut(s) 1683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.