Rroxscaffold_1G00046090

Protein of unknown function (DUF2921)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000420
Physical Location & Seq
Forward (+)
65219513 .. 65223896
4384 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00046090.1

Sequence Viewer

Length: 2436 bp
ATGGCGCTTCTGGTGAATTCAAGTCACCAACAGCATCTCACTTTGGGTTCAAGCAGCTATGGTGCTTATGCTGGTTTGGTGTTGGATGGCTTTCAGTTTCCTCAAATTCTACTCAATGTGGTCTGCAAATCAAAGGAAAAATCTCTGTCAATTTGGTTCTACATCGAAACAACTTTTGTTCGAATCCTGCCACATGCATATGATCTTTACAGGACTAGCAGATATGATTTGGTCTTGGACGAGCCATATGTTTTTGCAACTCCTGTGGCTTGGATTTTCTCCACTGCTTGGGATGTCATTATTCCTATGGGAGCTCCATGTTTTCTGCAATCATTTTCTCACAGCAGAAGTTTGGGGGCCATTGCTTTCTTTCTCCAAAGTGGAGAGAGTTGGATACATATGAGAAGGTCCTCAAGTGACTCAAGCCTCATTCTGAAAGTGCACCAGAATCCAGCTGAAGCAGAAAATAGGGCTGTTCCTGGTGGCCATTGGGCAAGAATTATTTGTTTCTATATATATGCCAGTTTTGATCCCTCTGTCCTTAGCCATACAGGTTACTATTCTGGCGGTGGCAGTGGCATTCGTAGTCCAAAACGATCATATCAACCCAATTATGAGCCCTCATATCCTGTTGAGTTCAATGTCTGGAGCGTTGAAGAAACTGATGTACAAGACTTATTCGAGGTTTGTCATGGAAGTCTTCAATTTCAAAGAGACACTGACCACATGGGAAGCTTGACAAATCGCCTGCCTCAAAGTCCGATAAGATTTGCACTAAATGGATTCTGGTCACAATCTTCGGGAAAGCTTTGCATGGTGGGATCAGGTTCTATTTACTCCAAACAAGGTGACCTGCCTAGTGATGTTCCTGCTCTTCTCAAGTTGCATAATCTCATGAATTTCTCTAGTGTTACAAGTTTGATTAGTGGAACCCTGGAGAGCTTGATGATTTCTGAGAAAGATCCCAATTATTTTGAACCAATCTCTATTTTGATGTTTCCTCATATGAACTACCAGTACACTTTGGTTTCTAATAAATCTAATGATAATAGCTCCTCCAGTGAAAGTGATGCTCCCAAAGATTCTTTGCAAATGGAGAAATTTTGTTCTGTAATCTCAGTAGTACCAGAATATTATCTTCAGTATTCGCGCCATTGCCTTTCTGCAAAGAACTGCACTACTCCTCTTTTTGGGTCTGGGCTTTTCCCTAGCATTGTTGCATTAAGGGGTATCGAGTGTTCAGAAGATAACCAGAGGTTGAGAGTTCTGATAGAATTTGCAGACCAACGAAAACAATGGTATAACATGACTTTCAATCCCAATACAACTTTGGTTGGGGAGGGATCATGGGATGTAAACAAAACTCCGCTACAGCCTACAAGTTGTAGCATGTCATTTCTTGGACGCGAGAAATTGAGCTTGACATTTCCTGGAATATGGACAATTAAAGATACCAGAAATGCTGCAGGGCACATTTGGAGCAACAAAGCTGTGGCAGAGTCCGGTTACTTTGAAAAGATTACATTTGGTAGTCTTATGAATTATGATGGAGGCGTGCAACGAGCAGGTCAGAAATATGAGTATACCCAAATTTTAAAGGTAACCAAGTTCTGCCCCAGAGAGAAGAGCGCTGCTGCCACTGATTACAAGACCAAGGTATACCCAAATCCCTTTTTATATGACATGAGGTTCGATATGTTATCTAAAAGATCTAAAAGCATAGTGGATCAGGGCAGTTCTTATCCTCTTTCTGTTGGAAATCGGTTGTTCAGATATGGAACATCTATTGAAGATTCGTACTCACTTGCATATACCCACCGCGGCCCTTACAATATTAGCTTCTTTATATTCATAAATGTGAGTAATATGGCAATTGCAAATGTGATATCTGCTGAAGGAGTCTATGATGATACAGATGGAAGCATGTGCATGGTAGGCTGCCGAAATCTAGGCCAGATAGATCAACAGCCAACTGATGATTCTGTAGATTGTGAGATTCTCCTCAAACTTCAGTTGCCTCCAGCAACTCCAGGGAGGAAGATCTCAGGTTATGTCGAGGGGAGCATTGAAAGCATAAGGAAAAAGTCTGATCCTCTTCATTTTGAACGTTTAGACTTGTCTTCAGATGACATGTTTATGGTTGAAGCAGATCGATCCATTCGTAGGTTGGAGGTGGAGACCACCTTGGTTCTTATATCCACCACACTTGCATATGTTTTTGGGGCATTACAACTCTTCCATATGAAGAAACATCCAGATGCTGTTCACATTCTAAAGCATTTTGCTTGGGGTCGTTTAAGATCATATGCTGTTCTAGTACCCGATAGCTTTGTATTTCCTCTAGTCCTCCTCAACGTCTTGCAAAACTCAAAGGAAAATGCTCTTTCTTGTTACCTTGGAATTACATTTTATGTATGTGCTTCCACATGCGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
DUF2921

Protein Analysis

811

Amino Acids

91.15

Weight (kDa)

6.38

Isoelectric Point (pI)

49.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2921 PF11145 18 - 103 5.2e-15 Transmembrane E3 ligase/DUF2921 transmembrane domain
DUF2921_N PF25333 182 - 329 7.3e-17 DUF2921 lipocalin-like domain
DUF2921_N PF25333 402 - 508 6e-09 DUF2921 lipocalin-like domain
DUF2921_N PF25333 534 - 705 2.7e-27 DUF2921 lipocalin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21700
fragaria_vesca FvH4_1g10360 FvH4_1g10760 FvH4_2g30370 FvH4_3g09240 FvH4_3g20050 FvH4_3g20280 FvH4_3g20290 FvH4_3g20291 FvH4_3g20340 FvH4_3g20350 FvH4_3g20351 FvH4_3g20380 FvH4_3g20390 FvH4_5g13190 FvH4_6g20070 FvH4_6g42890
malus_domestica MD03G1227000.v1.1 MD10G1294900.v1.1 MD11G1246300.v1.1 MD11G1246400.v1.1 MD11G1246500.v1.1 MD11G1246600.v1.1
prunus_persica Prupe.4G047300_v2.0.a1 Prupe.4G181700_v2.0.a1 Prupe.4G181900_v2.0.a1 Prupe.4G182000_v2.0.a1 Prupe.4G182200_v2.0.a1
pyrus_communis pycom03g17470 pycom10g24670 pycom11g21640
rosa_chinensis RchiOBHm_Chr5g0008381 RchiOBHm_Chr5g0008391 RchiOBHm_Chr5g0033881 RchiOBHm_Chr5g0034171 RchiOBHm_Chr5g0034181 RchiOBHm_Chr5g0034191 RchiOBHm_Chr5g0034201 RchiOBHm_Chr5g0034211 RchiOBHm_Chr5g0034221 RchiOBHm_Chr5g0034231 RchiOBHm_Chr5g0034271 RchiOBHm_Chr5g0034281
rosa_laevigata RLG00000031566 RLG00000031567 RLG00000033506 RLG00000033529 RLG00000033530 RLG00000033531
rosa_multiflora Rmu_co8445509.1_g000001 Rmu_co8470615.1_g000001 Rmu_sc0008805.1_g000003 Rmu_sc0008805.1_g000004 Rmu_ssc0000128.1_g000029 Rmu_ssc0000128.1_g000031 Rmu_ssc0000128.1_g000032 Rmu_ssc0000388.1_g000062 Rmu_ssc0000388.1_g000063
rosa_roxburghii Rroxscaffold_1G00046010 Rroxscaffold_1G00046020 Rroxscaffold_1G00046030 Rroxscaffold_1G00046040 Rroxscaffold_1G00046060 Rroxscaffold_1G00046070 Rroxscaffold_1G00046080 Rroxscaffold_1G00046090
rosa_rugosa Rorug04G0435500 Rorug05G0142900 Rorug05G0144600 Rorug05G0144600 Rorug05G0144700 Rorug05G0144800
rosa_samantha Rh5AG233800 Rh5AG235900 Rh5AG236000 Rh5AG236100 Rh5AG236200 Rh5AG236300 Rh5AG236500 Rh5BG061200 Rh5BG061300 Rh5BG234300 Rh5BG236600 Rh5BG236800 Rh5CG071200 Rh5DG060000 Rh5DG060100 Rh5DG241300 Rh5DG243700 Rh5DG244000 Rh5DG244100
rosa_wichuraiana Rw3G018950 Rw5G005680 Rw5G021330 Rw5G021340 Rw5G021350 Rw5G021550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 861, 1556
AccB7I CCANNNNNTGG 1 cut(s) 288
AccI GTMKAC 2 cut(s) 1583, 1659
AccII CGCG 3 cut(s) 1150, 1407, 1821
AciI CCGC 4 cut(s) 567, 1367, 1819, 1821
AclI AACGTT 1 cut(s) 2107
AclWI GGATC 7 cut(s) 524, 829, 956, 1351, 1734, 2084, 2148
AcoI YGGCCR 1 cut(s) 484
AcsI RAATTY 6 cut(s) 16, 105, 898, 1100, 1274, 1590
AcuI CTGAAG 5 cut(s) 477, 1124, 1914, 1994, 2106
AfaI GTAC 5 cut(s) 669, 1019, 1125, 1799, 2319
AfeI AGCGCT 1 cut(s) 1630
AfiI CCNNNNNNNGG 3 cut(s) 288, 1190, 2163
AflIII ACRYGT 1 cut(s) 2130
AjnI CCWGG 4 cut(s) 478, 933, 1429, 2029
AjuI GAANNNNNNNTTGG 2 cut(s) 1963, 1995
Alw21I GWGCWC 2 cut(s) 316, 444
Alw26I GTCTC 2 cut(s) 708, 2171
Alw44I GTGCAC 1 cut(s) 440
AlwI GGATC 7 cut(s) 524, 829, 956, 1351, 1734, 2084, 2148
AlwNI CAGNNNCTG 1 cut(s) 2261
Aor51HI AGCGCT 1 cut(s) 1630
AoxI GGCC 4 cut(s) 357, 484, 1822, 1951
ApaLI GTGCAC 1 cut(s) 440
ApeKI GCWGC 5 cut(s) 54, 1463, 1631, 1634, 1938
ApoI RAATTY 6 cut(s) 16, 105, 898, 1100, 1274, 1590
ArsI GACNNNNNNTTYG 2 cut(s) 1673, 1705
AspLEI GCGC 3 cut(s) 7, 1152, 1631
AspS9I GGNCC 3 cut(s) 357, 408, 1823
AsuHPI GGTGA 3 cut(s) 17, 25, 860
AsuII TTCGAA 1 cut(s) 181
AvaII GGWCC 1 cut(s) 408
BaeGI GKGCMC 2 cut(s) 444, 1473
BalI TGGCCA 1 cut(s) 486
BanII GRGCYC 2 cut(s) 316, 621
BbsI GAAGAC 2 cut(s) 692, 2112
Bbv12I GWGCWC 2 cut(s) 316, 444
BbvI GCAGC 5 cut(s) 66, 1450, 1618, 1621, 1925
BccI CCATC 3 cut(s) 80, 1541, 1910
BciT130I CCWGG 4 cut(s) 480, 935, 1431, 2031
BciVI GTATCC 1 cut(s) 387
BcoDI GTCTC 2 cut(s) 708, 2171
BfaI CTAG 7 cut(s) 216, 858, 906, 1209, 1949, 2315, 2342
BfmI CTRYAG 3 cut(s) 1370, 1464, 1983
BfoI RGCGCY 2 cut(s) 8, 1632
BfuAI ACCTGC 2 cut(s) 861, 1556
BfuI GTATCC 1 cut(s) 387
BglII AGATCT 2 cut(s) 1709, 2040
BisI GCNGC 6 cut(s) 55, 1464, 1632, 1635, 1822, 1939
BlsI GCNGC 6 cut(s) 56, 1465, 1633, 1636, 1823, 1940
Bme1390I CCNGG 4 cut(s) 480, 935, 1431, 2031
Bme18I GGWCC 1 cut(s) 408
BmgT120I GGNCC 3 cut(s) 357, 408, 1823
BmiI GGNNCC 2 cut(s) 358, 931
BmrFI CCNGG 4 cut(s) 480, 935, 1431, 2031
BmsI GCATC 3 cut(s) 43, 1060, 2248
BpiI GAAGAC 2 cut(s) 692, 2112
BpmI CTGGAG 5 cut(s) 667, 956, 1042, 2004, 2013
Bpu10I CCTNAGC 1 cut(s) 542
Bpu14I TTCGAA 1 cut(s) 181
BpuEI CTTGAG 3 cut(s) 397, 406, 863
Bsa29I ATCGAT 1 cut(s) 2152
BsaI GGTCTC 1 cut(s) 2171
BsaJI CCNNGG 6 cut(s) 933, 1653, 1819, 2030, 2184, 2395
BsaWI WCCGGW 1 cut(s) 1502
Bsc4I CCNNNNNNNGG 3 cut(s) 288, 1190, 2163
Bse1I ACTGG 3 cut(s) 522, 1015, 1059
Bse3DI GCAATG 2 cut(s) 360, 1153
BseBI CCWGG 4 cut(s) 480, 935, 1431, 2031
BseCI ATCGAT 1 cut(s) 2152
BseDI CCNNGG 6 cut(s) 933, 1653, 1819, 2030, 2184, 2395
BseGI GGATG 4 cut(s) 91, 298, 1357, 2251
BseLI CCNNNNNNNGG 3 cut(s) 288, 1190, 2163
BseMI GCAATG 2 cut(s) 360, 1153
BseMII CTCAG 3 cut(s) 945, 1131, 2058
BseNI ACTGG 3 cut(s) 522, 1015, 1059
BseRI GAGGAG 4 cut(s) 1045, 1173, 1991, 2339
BseSI GKGCMC 2 cut(s) 444, 1473
BseXI GCAGC 5 cut(s) 66, 1450, 1618, 1621, 1925
BsgI GTGCAG 1 cut(s) 1159
Bsh1236I CGCG 3 cut(s) 1150, 1407, 1821
BshFI GGCC 4 cut(s) 359, 486, 1824, 1953
BshVI ATCGAT 1 cut(s) 2152
BsiHKAI GWGCWC 2 cut(s) 316, 444
BsiSI CCGG 1 cut(s) 1503
BslI CCNNNNNNNGG 3 cut(s) 288, 1190, 2163
BsmAI GTCTC 2 cut(s) 708, 2171
BsmI GAATGC 1 cut(s) 579
BsnI GGCC 4 cut(s) 359, 486, 1824, 1953
Bso31I GGTCTC 1 cut(s) 2171
Bsp119I TTCGAA 1 cut(s) 181
Bsp1286I GDGCHC 4 cut(s) 316, 444, 621, 1473
Bsp1407I TGTACA 1 cut(s) 667
BspACI CCGC 4 cut(s) 567, 1367, 1819, 1821
BspANI GGCC 4 cut(s) 359, 486, 1824, 1953
BspCNI CTCAG 3 cut(s) 946, 1130, 2057
BspDI ATCGAT 1 cut(s) 2152
BspFNI CGCG 3 cut(s) 1150, 1407, 1821
BspHI TCATGA 1 cut(s) 894
BspLI GGNNCC 2 cut(s) 358, 931
BspMAI CTGCAG 1 cut(s) 1468
BspMI ACCTGC 2 cut(s) 861, 1556
BspPI GGATC 7 cut(s) 524, 829, 956, 1351, 1734, 2084, 2148
BspQI GCTCTTC 2 cut(s) 879, 1619
BspT104I TTCGAA 1 cut(s) 181
BspTNI GGTCTC 1 cut(s) 2171
BsrDI GCAATG 2 cut(s) 360, 1153
BsrGI TGTACA 1 cut(s) 667
BsrI ACTGG 3 cut(s) 522, 1015, 1059
BssECI CCNNGG 6 cut(s) 933, 1653, 1819, 2030, 2184, 2395
BssNAI GTATAC 2 cut(s) 1584, 1660
BssT1I CCWWGG 3 cut(s) 1653, 2184, 2395
Bst1107I GTATAC 2 cut(s) 1584, 1660
Bst2UI CCWGG 4 cut(s) 480, 935, 1431, 2031
Bst6I CTCTTC 4 cut(s) 879, 1619, 2100, 2240
BstAUI TGTACA 1 cut(s) 667
BstBI TTCGAA 1 cut(s) 181
BstC8I GCNNGC 2 cut(s) 749, 1556
BstDEI CTNAG 4 cut(s) 542, 954, 1117, 2044
BstDSI CCRYGG 1 cut(s) 1819
BstEII GGTNACC 2 cut(s) 848, 1600
BstF5I GGATG 4 cut(s) 91, 298, 1357, 2251
BstFNI CGCG 3 cut(s) 1150, 1407, 1821
BstH2I RGCGCY 2 cut(s) 8, 1632
BstHHI GCGC 3 cut(s) 7, 1152, 1631
BstMAI GTCTC 2 cut(s) 708, 2171
BstMWI GCNNNNNNNGC 2 cut(s) 1935, 2070
BstNI CCWGG 4 cut(s) 480, 935, 1431, 2031
BstNSI RCATGY 5 cut(s) 197, 1393, 1927, 2134, 2430
BstPI GGTNACC 2 cut(s) 848, 1600
BstSCI CCNGG 4 cut(s) 478, 933, 1429, 2029
BstSFI CTRYAG 3 cut(s) 1370, 1464, 1983
BstSLI GKGCMC 2 cut(s) 444, 1473
BstUI CGCG 3 cut(s) 1150, 1407, 1821
BstV1I GCAGC 5 cut(s) 66, 1450, 1618, 1621, 1925
BstV2I GAAGAC 2 cut(s) 692, 2112
BstX2I RGATCY 3 cut(s) 961, 1709, 2040
BstYI RGATCY 3 cut(s) 961, 1709, 2040
BstZ17I GTATAC 2 cut(s) 1584, 1660
Bsu15I ATCGAT 1 cut(s) 2152
BsuI GTATCC 1 cut(s) 387
BsuRI GGCC 4 cut(s) 359, 486, 1824, 1953
BsuTUI ATCGAT 1 cut(s) 2152
BtgI CCRYGG 1 cut(s) 1819
BtsCI GGATG 4 cut(s) 91, 298, 1357, 2251
BtsI GCAGTG 2 cut(s) 282, 580
BtsIMutI CAGTG 5 cut(s) 282, 580, 717, 1066, 1638
BveI ACCTGC 2 cut(s) 861, 1556
Cac8I GCNNGC 2 cut(s) 749, 1556
CaiI CAGNNNCTG 1 cut(s) 2261
CciI TCATGA 1 cut(s) 894
CfoI GCGC 3 cut(s) 7, 1152, 1631
Cfr13I GGNCC 3 cut(s) 357, 408, 1823
Cfr42I CCGCGG 1 cut(s) 1822
ClaI ATCGAT 1 cut(s) 2152
CseI GACGC 1 cut(s) 1413
Csp6I GTAC 5 cut(s) 668, 1018, 1124, 1798, 2318
CspCI CAANNNNNGTGG 4 cut(s) 246, 281, 2188, 2223
CviQI GTAC 5 cut(s) 668, 1018, 1124, 1798, 2318
DdeI CTNAG 4 cut(s) 542, 954, 1117, 2044
DraI TTTAAA 1 cut(s) 1596
EaeI YGGCCR 1 cut(s) 484
Eam1104I CTCTTC 4 cut(s) 879, 1619, 2100, 2240
EarI CTCTTC 4 cut(s) 879, 1619, 2100, 2240
Ecl136II GAGCTC 1 cut(s) 314
Eco130I CCWWGG 3 cut(s) 1653, 2184, 2395
Eco24I GRGCYC 2 cut(s) 316, 621
Eco31I GGTCTC 1 cut(s) 2171
Eco32I GATATC 1 cut(s) 1887
Eco47I GGWCC 1 cut(s) 408
Eco47III AGCGCT 1 cut(s) 1630
Eco53kI GAGCTC 1 cut(s) 314
Eco57I CTGAAG 5 cut(s) 477, 1124, 1914, 1994, 2106
Eco91I GGTNACC 2 cut(s) 848, 1600
EcoICRI GAGCTC 1 cut(s) 314
EcoO109I RGGNCCY 1 cut(s) 408
EcoO65I GGTNACC 2 cut(s) 848, 1600
EcoRI GAATTC 1 cut(s) 16
EcoRII CCWGG 4 cut(s) 478, 933, 1429, 2029
EcoRV GATATC 1 cut(s) 1887
EcoT14I CCWWGG 3 cut(s) 1653, 2184, 2395
EcoT22I ATGCAT 1 cut(s) 199
EcoT38I GRGCYC 2 cut(s) 316, 621
ErhI CCWWGG 3 cut(s) 1653, 2184, 2395
FauNDI CATATG 7 cut(s) 199, 247, 399, 1005, 2212, 2241, 2305
FblI GTMKAC 2 cut(s) 1583, 1659
Fnu4HI GCNGC 6 cut(s) 55, 1464, 1632, 1635, 1822, 1939
FokI GGATG 4 cut(s) 98, 305, 1364, 2238
FriOI GRGCYC 2 cut(s) 316, 621
Fsp4HI GCNGC 6 cut(s) 55, 1464, 1632, 1635, 1822, 1939
FspBI CTAG 7 cut(s) 216, 858, 906, 1209, 1949, 2315, 2342
GlaI GCGC 3 cut(s) 6, 1151, 1630
GluI GCNGC 6 cut(s) 55, 1464, 1632, 1635, 1822, 1939
GsuI CTGGAG 5 cut(s) 667, 956, 1042, 2004, 2013
HaeII RGCGCY 2 cut(s) 8, 1632
HaeIII GGCC 4 cut(s) 359, 486, 1824, 1953
HapII CCGG 1 cut(s) 1503
HgaI GACGC 1 cut(s) 1413
HhaI GCGC 3 cut(s) 7, 1152, 1631
Hin6I GCGC 3 cut(s) 5, 1150, 1629
HinP1I GCGC 3 cut(s) 5, 1150, 1629
HindIII AAGCTT 2 cut(s) 733, 806
HpaII CCGG 1 cut(s) 1503
HphI GGTGA 3 cut(s) 17, 25, 860
Hpy166II GTNNAC 6 cut(s) 442, 1020, 1357, 1584, 1660, 2266
Hpy188I TCNGA 9 cut(s) 435, 762, 955, 1243, 1269, 1572, 1772, 2089, 2125
Hpy188III TCNNGA 4 cut(s) 646, 801, 895, 2255
Hpy8I GTNNAC 6 cut(s) 442, 1020, 1357, 1584, 1660, 2266
HpyAV CCTTC 2 cut(s) 399, 1889
HpyCH4IV ACGT 2 cut(s) 2107, 2355
HpyF10VI GCNNNNNNNGC 2 cut(s) 1935, 2070
HpyF3I CTNAG 4 cut(s) 542, 954, 1117, 2044
HpySE526I ACGT 2 cut(s) 2107, 2355
HspAI GCGC 3 cut(s) 5, 1150, 1629
KspI CCGCGG 1 cut(s) 1822
LguI GCTCTTC 2 cut(s) 879, 1619
LmnI GCTCC 7 cut(s) 311, 319, 648, 1058, 1078, 1479, 2061
Lsp1109I GCAGC 5 cut(s) 66, 1450, 1618, 1621, 1925
LweI GCATC 3 cut(s) 43, 1060, 2248
MaeI CTAG 7 cut(s) 216, 858, 906, 1209, 1949, 2315, 2342
MaeII ACGT 2 cut(s) 2107, 2355
MaeIII GTNAC 9 cut(s) 23, 416, 554, 789, 848, 910, 1505, 1600, 2390
MfeI CAATTG 1 cut(s) 1872
MflI RGATCY 3 cut(s) 961, 1709, 2040
MhlI GDGCHC 4 cut(s) 316, 444, 621, 1473
MlsI TGGCCA 1 cut(s) 486
MluNI TGGCCA 1 cut(s) 486
MlyI GAGTC 3 cut(s) 413, 1508, 1908
MmeI TCCRAC 4 cut(s) 63, 371, 1735, 2148
Mox20I TGGCCA 1 cut(s) 486
Mph1103I ATGCAT 1 cut(s) 199
MscI TGGCCA 1 cut(s) 486
MseI TTAA 4 cut(s) 1223, 1446, 1595, 2297
MslI CAYNNNNRTG 6 cut(s) 198, 1856, 1928, 2135, 2256, 2431
Msp20I TGGCCA 1 cut(s) 486
MspA1I CMGCKG 2 cut(s) 455, 1821
MspI CCGG 1 cut(s) 1503
MspR9I CCNGG 4 cut(s) 480, 935, 1431, 2031
MunI CAATTG 1 cut(s) 1872
Mva1269I GAATGC 1 cut(s) 579
MvaI CCWGG 4 cut(s) 480, 935, 1431, 2031
MvnI CGCG 3 cut(s) 1150, 1407, 1821
MwoI GCNNNNNNNGC 2 cut(s) 1935, 2070
NdeI CATATG 7 cut(s) 199, 247, 399, 1005, 2212, 2241, 2305
NlaIV GGNNCC 2 cut(s) 358, 931
NmuCI GTSAC 4 cut(s) 23, 416, 789, 848
NsiI ATGCAT 1 cut(s) 199
NspI RCATGY 5 cut(s) 197, 1393, 1927, 2134, 2430
NspV TTCGAA 1 cut(s) 181
PagI TCATGA 1 cut(s) 894
PciI ACATGT 1 cut(s) 2130
PciSI GCTCTTC 2 cut(s) 879, 1619
PcsI WCGNNNNNNNCGW 1 cut(s) 2158
PctI GAATGC 1 cut(s) 579
PfeI GAWTC 7 cut(s) 183, 448, 783, 1082, 1793, 1979, 1996
PflMI CCANNNNNTGG 1 cut(s) 288
PfoI TCCNGGA 1 cut(s) 1429
PkrI GCNGC 6 cut(s) 56, 1465, 1633, 1636, 1823, 1940
PleI GAGTC 3 cut(s) 413, 1507, 1907
PpsI GAGTC 3 cut(s) 413, 1507, 1907
PpuMI RGGWCCY 1 cut(s) 408
PscI ACATGT 1 cut(s) 2130
Psp124BI GAGCTC 1 cut(s) 316
Psp1406I AACGTT 1 cut(s) 2107
Psp5II RGGWCCY 1 cut(s) 408
Psp6I CCWGG 4 cut(s) 478, 933, 1429, 2029
PspEI GGTNACC 2 cut(s) 848, 1600
PspGI CCWGG 4 cut(s) 478, 933, 1429, 2029
PspN4I GGNNCC 2 cut(s) 358, 931
PspPI GGNCC 3 cut(s) 357, 408, 1823
PspPPI RGGWCCY 1 cut(s) 408
PsrI GAACNNNNNNTAC 4 cut(s) 1001, 1033, 1222, 1254
PstI CTGCAG 1 cut(s) 1468
PstNI CAGNNNCTG 1 cut(s) 2261
PsuI RGATCY 3 cut(s) 961, 1709, 2040
PvuII CAGCTG 1 cut(s) 455
RsaI GTAC 5 cut(s) 669, 1019, 1125, 1799, 2319
RsaNI GTAC 5 cut(s) 668, 1018, 1124, 1798, 2318
RseI CAYNNNNRTG 6 cut(s) 198, 1856, 1928, 2135, 2256, 2431
SacI GAGCTC 1 cut(s) 316
SacII CCGCGG 1 cut(s) 1822
SapI GCTCTTC 2 cut(s) 879, 1619
SaqAI TTAA 4 cut(s) 1223, 1446, 1595, 2297
SatI GCNGC 6 cut(s) 55, 1464, 1632, 1635, 1822, 1939
Sau96I GGNCC 3 cut(s) 357, 408, 1823
SchI GAGTC 3 cut(s) 413, 1508, 1908
ScrFI CCNGG 4 cut(s) 480, 935, 1431, 2031
SduI GDGCHC 4 cut(s) 316, 444, 621, 1473
SfaNI GCATC 3 cut(s) 43, 1060, 2248
SfcI CTRYAG 3 cut(s) 1370, 1464, 1983
Sfr303I CCGCGG 1 cut(s) 1822
SfuI TTCGAA 1 cut(s) 181
SgrBI CCGCGG 1 cut(s) 1822
SinI GGWCC 1 cut(s) 408
SmiMI CAYNNNNRTG 6 cut(s) 198, 1856, 1928, 2135, 2256, 2431
SmlI CTYRAG 3 cut(s) 412, 421, 878
SmoI CTYRAG 3 cut(s) 412, 421, 878
SsiI CCGC 4 cut(s) 567, 1367, 1819, 1821
SspI AATATT 2 cut(s) 1133, 1834
SspMI CTAG 7 cut(s) 216, 858, 906, 1209, 1949, 2315, 2342
SstI GAGCTC 1 cut(s) 316
StyD4I CCNGG 4 cut(s) 478, 933, 1429, 2029
StyI CCWWGG 3 cut(s) 1653, 2184, 2395
TaiI ACGT 2 cut(s) 2110, 2358
TaqI TCGA 7 cut(s) 165, 181, 681, 1233, 1692, 2055, 2152
TatI WGTACW 2 cut(s) 667, 1017
TauI GCSGC 1 cut(s) 1824
TfiI GAWTC 7 cut(s) 183, 448, 783, 1082, 1793, 1979, 1996
Tru1I TTAA 4 cut(s) 1223, 1446, 1595, 2297
Tru9I TTAA 4 cut(s) 1223, 1446, 1595, 2297
TscAI CASTG 5 cut(s) 289, 580, 724, 1066, 1645
TseFI GTSAC 4 cut(s) 23, 416, 789, 848
TseI GCWGC 5 cut(s) 54, 1463, 1631, 1634, 1938
Tsp45I GTSAC 4 cut(s) 23, 416, 789, 848
TspDTI ATGAA 6 cut(s) 911, 1022, 1553, 1840, 2087, 2258
TspRI CASTG 5 cut(s) 289, 580, 724, 1066, 1645
Van91I CCANNNNNTGG 1 cut(s) 288
VneI GTGCAC 1 cut(s) 440
VpaK11BI GGWCC 1 cut(s) 408
XapI RAATTY 6 cut(s) 16, 105, 898, 1100, 1274, 1590
XceI RCATGY 5 cut(s) 197, 1393, 1927, 2134, 2430
XcmI CCANNNNNNNNNTGG 2 cut(s) 1327, 2164
XmiI GTMKAC 2 cut(s) 1583, 1659
XspI CTAG 7 cut(s) 216, 858, 906, 1209, 1949, 2315, 2342
Zsp2I ATGCAT 1 cut(s) 199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.