MD11G1246600.v1.1

Protein of unknown function (DUF2921)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000420
Physical Location & Seq
Reverse (-)
35841661 .. 35844414
2754 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1246600.v1.1.491

Sequence Viewer

Length: 2754 bp
ATGACCAAAGAATCCATGGCTGTCTTTCTCATGTTCTTCTTTCTTTTCTTCGCCACCTCTTCTGCAGCCTCTTCTAAATTCGATCTCCAAACAGCTTACCCTGACCACTGTGCTTCATTTGTTCCCCAGTCTACCCCGAAAGGAGACGCGCAAGGACCGCCCGACCAACACCGCCACACAGGTTACTACACCCGTGGCGGTGGCAGTGACGTTCTTAGTCTTAGCTCATTGTATTATCCTAACGAAAACTCTGTTCAATTCGTTATCAAGAGCATTAGAGAAACTGATGTTCAAGGCCTGATCAAGCTTCAAGGGAGTTTGATATTTCCGAGAGATGCGTTTTACTTTGTGGGAAGTACTTCCACCACAAGTAAGATGTCCTCATCTCGATCAGTTCTTCGTCCAAGAAGATCAATAAGTGTTACACTTGGTGGGTTCTGGTCACAATCTTCCGGGAAGCTTTGTATGGTTGGTTCGGGTTATGATTACTTGGAAGGACGCAGTTATTTGCATCACATTCATTCTGTTCTTAAGCTCTACAATTTCGCGAACTCCACTAAAGCCACTAGTTTGATTAGCGGAACCTTAGAGAGCATGAACACTTCCGAGAATGATGCAGATTATTTCGAACCAATCTCTATTTTGATGCTTCCTAGCATGAACTACCAGTACACTCTGATTTCAAATAAATCTGAGACCATCAGTACTTCCGGTGGAACTGATAGTTCCAATCCCACAAGTTCCTTGAATAGAAAGAGATTTTGTTCTGTACTCTCAAACGAAGTGCCATACCATGCATTTGATCTCAAATACTCAAGCCGTTGCGTATCTGCGAACAACTGCACTCCAGTTGCTGCGTCTAGTTTGCCTCGGTTTATGTTCTTGAAGCTTACTGAATGTTTGGAGGATACACGAAGGTTGAGAGTCCTGGTGGAATTTGAAGACAGCAGCAACTTCTGGTATCGAACGTTCGATCCCAATACAACACTGGTTGGGGAAGGCTCATGGGATGCAGAGAAAAATCAGCTATCTTTTGTTGCATGTCAATTCTTGGTTGCAGCAGGCTCTTGGAACAATACTAGCGTCGGTGATTGTTCAATAAGATTGAGCTTGCGATTTTCAGCTGTTTGGACAATCGGAAACAGTAGTAGCATGGTGGGGAAGATTTGGAGCAACAAAACTGTGACAGAGTCGGGTTACTTTGAAAAGATCACGTTCGAAAGTCTTGAGGATGACATGGGAAGGACTCTGATTCCAGGCGCGAAGTATGAGTACACGAAAATCAACACCGTAAACAAGTTGTGCCCGAAAAAGGAGACTGCTCGTGGCAAGACTAATGTGTACGCGGATCCGTTTTCTTATGAAATGAGATTTGATGTGTCAGCTAAGAATTCTGAAGGACAAGTTGCATGGGGCAGTTCTGTCCCGATTTCTGTTGGGGATCAATTTTATCAGGCGTATCAGTATTCGATTGCAAGCGCTGAAGATGAAGATGAATCTACAGCTCCTGAGAGATACAGCTTCAACCACAGCAAAGCGTACAATATTAGCTACAAAATAAGCATCAACCGGCTATCTGATGCAAAGTTTGGAAATCAATCAGTTTTAAACGAAATGCAAATCTTTGCCGAAGGGATCTATGATGAAACCGAAGGCAGCCTGTGCATGGTAGGCTGCAGAAAACTGGCCTCAAAGGGTCAGCAGCCAACAAATGACTCTGTAGATTGCGAGATTGCTGTCAACTTTCAGTTCCCTACAACAAACCCAACCAAGAGTTCGGGTCTCATCAAGGGCAGCGTAAAAAGCACACGAAAAAAGTCCGACCCTCTTCATTTTGAAACTTGGGATTTAACCTCAGCTTCCTTTACCCTCGGCGAAGAAAGGCTGTCCATTTGGAGGATGGATGTGGAGATCATATTGGTTCTTATATCCACCACGCTGGCGTGCTTCTTTGTGGCGCGACAACTCTTCCATGTGAAAAAGTATCCAGATGTTATTCCCTCCATTTCCATTTTCATGCTACTAATTCTAACTCTTGGCTACATGATACCCCTTATGCTAAACTTTGAAGCCATGTTCACAAATAGCACCAACCGCCAAAATGTGCTCCTTGGAACCGGAGGATGGCTTAAAGTTAATGAGGTAATTGTGAGGGTGATAACAATGGTAGCGCTCTTGCTGCAAATCCGTCTTCTGCAGCTAACTTGGTCAGCAAGATCAAAAAATGGAAATCGCAATGAGCTCTGGCTCATGGAGAAGAAGACTCTCTTTGTTGCCTTACTACTATATGTTGCAGGGGCTTTCGCCATTTTGCTTCTGCATAAACAGATTTGGAAGAAGAGCAACTACGCCGAGCACTCAATTTTCAGCATTGCCTTCAAATCTTACTCCGGTTTGGTTATGGACGGTTTTTTGATGCCTCAAATTGTGCTCAATATGTTCTGCAAGTCAAAAGAAAATGCTCTATCCATTTGGTTCTACGTAGGAACAACTTTTGTTCGAGCGCTGCCACATGCATACGATCTTTACAGAGCTCACAACTTTGCTCATCCCCAATTCAACGAGTTGTACCAATATGCAAGCCCTGCAGCAGATTTTTACTCCACTTCTTGGGATGTCATAATTCCTTTTGGGGGTTTAGTGTTTGCTGGTATCATTTTCTTGCAGCAGAAGTTTGGGGGTCGTTGCTTTCTCCCCAAAAAGTTGATAGAGTTGGGTGAATATGAGAAGGTCCCTACTATTACCGAAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
DUF2921

Protein Analysis

918

Amino Acids

102.93

Weight (kDa)

8.16

Isoelectric Point (pI)

35.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2921_N PF25333 33 - 213 3.6e-19 DUF2921 lipocalin-like domain
DUF2921_N PF25333 248 - 405 2.6e-25 DUF2921 lipocalin-like domain
DUF2921_N PF25333 432 - 614 1.9e-35 DUF2921 lipocalin-like domain
DUF2921 PF11145 630 - 902 6.5e-66 Transmembrane E3 ligase/DUF2921 transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21700
fragaria_vesca FvH4_1g10360 FvH4_1g10760 FvH4_2g30370 FvH4_3g09240 FvH4_3g20050 FvH4_3g20280 FvH4_3g20290 FvH4_3g20291 FvH4_3g20340 FvH4_3g20350 FvH4_3g20351 FvH4_3g20380 FvH4_3g20390 FvH4_5g13190 FvH4_6g20070 FvH4_6g42890
malus_domestica MD03G1227000.v1.1 MD10G1294900.v1.1 MD11G1246300.v1.1 MD11G1246400.v1.1 MD11G1246500.v1.1 MD11G1246600.v1.1
prunus_persica Prupe.4G047300_v2.0.a1 Prupe.4G181700_v2.0.a1 Prupe.4G181900_v2.0.a1 Prupe.4G182000_v2.0.a1 Prupe.4G182200_v2.0.a1
pyrus_communis pycom03g17470 pycom10g24670 pycom11g21640
rosa_chinensis RchiOBHm_Chr5g0008381 RchiOBHm_Chr5g0008391 RchiOBHm_Chr5g0033881 RchiOBHm_Chr5g0034171 RchiOBHm_Chr5g0034181 RchiOBHm_Chr5g0034191 RchiOBHm_Chr5g0034201 RchiOBHm_Chr5g0034211 RchiOBHm_Chr5g0034221 RchiOBHm_Chr5g0034231 RchiOBHm_Chr5g0034271 RchiOBHm_Chr5g0034281
rosa_laevigata RLG00000031566 RLG00000031567 RLG00000033506 RLG00000033529 RLG00000033530 RLG00000033531
rosa_multiflora Rmu_co8445509.1_g000001 Rmu_co8470615.1_g000001 Rmu_sc0008805.1_g000003 Rmu_sc0008805.1_g000004 Rmu_ssc0000128.1_g000029 Rmu_ssc0000128.1_g000031 Rmu_ssc0000128.1_g000032 Rmu_ssc0000388.1_g000062 Rmu_ssc0000388.1_g000063
rosa_roxburghii Rroxscaffold_1G00046010 Rroxscaffold_1G00046020 Rroxscaffold_1G00046030 Rroxscaffold_1G00046040 Rroxscaffold_1G00046060 Rroxscaffold_1G00046070 Rroxscaffold_1G00046080 Rroxscaffold_1G00046090
rosa_rugosa Rorug04G0435500 Rorug05G0142900 Rorug05G0144600 Rorug05G0144600 Rorug05G0144700 Rorug05G0144800
rosa_samantha Rh5AG233800 Rh5AG235900 Rh5AG236000 Rh5AG236100 Rh5AG236200 Rh5AG236300 Rh5AG236500 Rh5BG061200 Rh5BG061300 Rh5BG234300 Rh5BG236600 Rh5BG236800 Rh5CG071200 Rh5DG060000 Rh5DG060100 Rh5DG241300 Rh5DG243700 Rh5DG244000 Rh5DG244100
rosa_wichuraiana Rw3G018950 Rw5G005680 Rw5G021330 Rw5G021340 Rw5G021350 Rw5G021550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 2610
AccI GTMKAC 1 cut(s) 131
AccII CGCG 5 cut(s) 149, 548, 1262, 1346, 1960
AciI CCGC 6 cut(s) 158, 172, 198, 579, 1346, 2095
AclI AACGTT 1 cut(s) 968
AclWI GGATC 5 cut(s) 968, 1343, 1356, 1449, 1643
AcsI RAATTY 3 cut(s) 77, 935, 1390
AcuI CTGAAG 2 cut(s) 1416, 1503
AdeI CACNNNGTG 1 cut(s) 431
AfaI GTAC 8 cut(s) 358, 671, 706, 771, 1274, 1343, 1541, 2570
AfeI AGCGCT 3 cut(s) 1480, 2172, 2505
AfiI CCNNNNNNNGG 6 cut(s) 1312, 1666, 1896, 2124, 2610, 2633
AflII CTTAAG 1 cut(s) 530
AhlI ACTAGT 1 cut(s) 566
AjnI CCWGG 2 cut(s) 927, 1255
AjuI GAANNNNNNNTTGG 2 cut(s) 1759, 1791
AloI GAACNNNNNNTCC 2 cut(s) 958, 990
Alw21I GWGCWC 5 cut(s) 2109, 2244, 2358, 2433, 2536
Alw26I GTCTC 4 cut(s) 138, 689, 1310, 1787
AlwI GGATC 5 cut(s) 968, 1343, 1356, 1449, 1643
AlwNI CAGNNNCTG 2 cut(s) 854, 1508
Aor51HI AGCGCT 3 cut(s) 1480, 2172, 2505
AoxI GGCC 2 cut(s) 295, 1686
ApoI RAATTY 3 cut(s) 77, 935, 1390
Asp700I GAANNNNTTC 1 cut(s) 358
AspLEI GCGC 6 cut(s) 151, 1262, 1481, 1960, 2173, 2506
AspS9I GGNCC 2 cut(s) 155, 2731
AsuC2I CCSGG 1 cut(s) 454
AsuHPI GGTGA 3 cut(s) 1100, 2167, 2729
AsuII TTCGAA 2 cut(s) 627, 1218
AvaII GGWCC 2 cut(s) 155, 2731
BaeGI GKGCMC 1 cut(s) 1307
BamHI GGATCC 1 cut(s) 1348
BanII GRGCYC 2 cut(s) 2244, 2536
BarI GAAGNNNNNNTAC 4 cut(s) 1256, 1288, 2330, 2362
BauI CACGAG 1 cut(s) 1323
BbsI GAAGAC 3 cut(s) 948, 2183, 2267
Bbv12I GWGCWC 5 cut(s) 2109, 2244, 2358, 2433, 2536
BbvCI CCTCAGC 1 cut(s) 1855
BccI CCATC 3 cut(s) 707, 1894, 2118
BceAI ACGGC 1 cut(s) 804
BcgI CGANNNNNNTGC 2 cut(s) 596, 630
BciT130I CCWGG 2 cut(s) 929, 1257
BciVI GTATCC 2 cut(s) 901, 1995
BclI TGATCA 1 cut(s) 300
BcnI CCSGG 1 cut(s) 454
BcoDI GTCTC 4 cut(s) 138, 689, 1310, 1787
BcuI ACTAGT 1 cut(s) 566
BfaI CTAG 4 cut(s) 567, 654, 861, 1080
BfmI CTRYAG 6 cut(s) 63, 1500, 1675, 1719, 2195, 2586
BfoI RGCGCY 3 cut(s) 1482, 2174, 2507
BfrI CTTAAG 1 cut(s) 530
BfuI GTATCC 2 cut(s) 901, 1995
BmcAI AGTACT 2 cut(s) 358, 706
Bme1390I CCNGG 3 cut(s) 454, 929, 1257
Bme18I GGWCC 2 cut(s) 155, 2731
BmgT120I GGNCC 2 cut(s) 155, 2731
BmiI GGNNCC 4 cut(s) 583, 1350, 2116, 2733
BmrFI CCNGG 3 cut(s) 454, 929, 1257
BmrI ACTGGG 1 cut(s) 121
BmsI GCATC 8 cut(s) 325, 520, 604, 636, 1000, 1570, 1572, 2406
BmuI ACTGGG 1 cut(s) 121
BpiI GAAGAC 3 cut(s) 948, 2183, 2267
BpmI CTGGAG 1 cut(s) 831
Bpu10I CCTNAGC 1 cut(s) 1855
Bpu14I TTCGAA 2 cut(s) 627, 1218
BpuEI CTTGAG 2 cut(s) 799, 1247
BpuMI CCSGG 1 cut(s) 454
BsaAI YACGTR 1 cut(s) 2482
BsaI GGTCTC 2 cut(s) 689, 1787
BsaJI CCNNGG 5 cut(s) 15, 193, 869, 1870, 2110
BsaWI WCCGGW 3 cut(s) 710, 2117, 2390
Bsc4I CCNNNNNNNGG 6 cut(s) 1312, 1666, 1896, 2124, 2610, 2633
Bse118I RCCGGY 1 cut(s) 1569
Bse1I ACTGG 5 cut(s) 127, 667, 848, 993, 1689
Bse3DI GCAATG 2 cut(s) 2242, 2370
BseBI CCWGG 2 cut(s) 929, 1257
BseDI CCNNGG 5 cut(s) 15, 193, 869, 1870, 2110
BseGI GGATG 7 cut(s) 1015, 1237, 1905, 1909, 2129, 2548, 2620
BseLI CCNNNNNNNGG 6 cut(s) 1312, 1666, 1896, 2124, 2610, 2633
BseMI GCAATG 2 cut(s) 2242, 2370
BseMII CTCAG 3 cut(s) 684, 1500, 1869
BseNI ACTGG 5 cut(s) 127, 667, 848, 993, 1689
BseSI GKGCMC 1 cut(s) 1307
BsgI GTGCAG 1 cut(s) 826
Bsh1236I CGCG 5 cut(s) 149, 548, 1262, 1346, 1960
BshFI GGCC 2 cut(s) 297, 1688
BsiHKAI GWGCWC 5 cut(s) 2109, 2244, 2358, 2433, 2536
BsiSI CCGG 5 cut(s) 453, 711, 1570, 2118, 2391
BslFI GGGAC 2 cut(s) 1409, 2717
BslI CCNNNNNNNGG 6 cut(s) 1312, 1666, 1896, 2124, 2610, 2633
BsmAI GTCTC 4 cut(s) 138, 689, 1310, 1787
BsmBI CGTCTC 1 cut(s) 138
BsmFI GGGAC 2 cut(s) 1409, 2717
BsnI GGCC 2 cut(s) 297, 1688
Bso31I GGTCTC 2 cut(s) 689, 1787
Bsp119I TTCGAA 2 cut(s) 627, 1218
Bsp1286I GDGCHC 6 cut(s) 1307, 2109, 2244, 2358, 2433, 2536
Bsp19I CCATGG 1 cut(s) 15
Bsp68I TCGCGA 1 cut(s) 548
BspACI CCGC 6 cut(s) 158, 172, 198, 579, 1346, 2095
BspANI GGCC 2 cut(s) 297, 1688
BspCNI CTCAG 3 cut(s) 685, 1501, 1868
BspFNI CGCG 5 cut(s) 149, 548, 1262, 1346, 1960
BspLI GGNNCC 4 cut(s) 583, 1350, 2116, 2733
BspMAI CTGCAG 4 cut(s) 67, 1679, 2199, 2590
BspPI GGATC 5 cut(s) 968, 1343, 1356, 1449, 1643
BspQI GCTCTTC 1 cut(s) 2333
BspT104I TTCGAA 2 cut(s) 627, 1218
BspTI CTTAAG 1 cut(s) 530
BspTNI GGTCTC 2 cut(s) 689, 1787
BsrDI GCAATG 2 cut(s) 2242, 2370
BsrFI RCCGGY 1 cut(s) 1569
BsrI ACTGG 5 cut(s) 127, 667, 848, 993, 1689
BssAI RCCGGY 1 cut(s) 1569
BssECI CCNNGG 5 cut(s) 15, 193, 869, 1870, 2110
BssSI CACGAG 1 cut(s) 1323
BssT1I CCWWGG 2 cut(s) 15, 2110
Bst2BI CACGAG 1 cut(s) 1323
Bst2UI CCWGG 2 cut(s) 929, 1257
Bst4CI ACNGT 5 cut(s) 110, 1145, 1183, 1291, 2408
Bst6I CTCTTC 5 cut(s) 64, 76, 1833, 1973, 2333
BstAFI CTTAAG 1 cut(s) 530
BstAPI GCANNNNNTGC 2 cut(s) 1662, 2585
BstBAI YACGTR 1 cut(s) 2482
BstBI TTCGAA 2 cut(s) 627, 1218
BstC8I GCNNGC 6 cut(s) 1063, 1112, 1477, 1941, 1945, 2581
BstDEI CTNAG 7 cut(s) 215, 221, 586, 693, 1386, 1509, 1855
BstDSI CCRYGG 2 cut(s) 15, 193
BstF5I GGATG 7 cut(s) 1015, 1237, 1905, 1909, 2129, 2548, 2620
BstFNI CGCG 5 cut(s) 149, 548, 1262, 1346, 1960
BstH2I RGCGCY 3 cut(s) 1482, 2174, 2507
BstHHI GCGC 6 cut(s) 151, 1262, 1481, 1960, 2173, 2506
BstMAI GTCTC 4 cut(s) 138, 689, 1310, 1787
BstMWI GCNNNNNNNGC 7 cut(s) 157, 1662, 1671, 1803, 2094, 2179, 2585
BstNI CCWGG 2 cut(s) 929, 1257
BstNSI RCATGY 2 cut(s) 1044, 2516
BstSCI CCNGG 3 cut(s) 452, 927, 1255
BstSFI CTRYAG 6 cut(s) 63, 1500, 1675, 1719, 2195, 2586
BstSLI GKGCMC 1 cut(s) 1307
BstSNI TACGTA 1 cut(s) 2482
BstUI CGCG 5 cut(s) 149, 548, 1262, 1346, 1960
BstV2I GAAGAC 3 cut(s) 948, 2183, 2267
BstX2I RGATCY 2 cut(s) 1348, 1635
BstXI CCANNNNNNTGG 1 cut(s) 1939
BstYI RGATCY 2 cut(s) 1348, 1635
BsuI GTATCC 2 cut(s) 901, 1995
BsuRI GGCC 2 cut(s) 297, 1688
BtgI CCRYGG 2 cut(s) 15, 193
BtsCI GGATG 7 cut(s) 1015, 1237, 1905, 1909, 2129, 2548, 2620
BtsI GCAGTG 1 cut(s) 211
BtsIMutI CAGTG 3 cut(s) 106, 211, 986
BtuMI TCGCGA 1 cut(s) 548
Cac8I GCNNGC 6 cut(s) 1063, 1112, 1477, 1941, 1945, 2581
CaiI CAGNNNCTG 2 cut(s) 854, 1508
CfoI GCGC 6 cut(s) 151, 1262, 1481, 1960, 2173, 2506
Cfr10I RCCGGY 1 cut(s) 1569
Cfr13I GGNCC 2 cut(s) 155, 2731
CseI GACGC 4 cut(s) 155, 507, 846, 1072
Csp6I GTAC 8 cut(s) 357, 670, 705, 770, 1273, 1342, 1540, 2569
CviQI GTAC 8 cut(s) 357, 670, 705, 770, 1273, 1342, 1540, 2569
DdeI CTNAG 7 cut(s) 215, 221, 586, 693, 1386, 1509, 1855
DraI TTTAAA 1 cut(s) 1608
DraIII CACNNNGTG 1 cut(s) 431
Eam1104I CTCTTC 5 cut(s) 64, 76, 1833, 1973, 2333
EarI CTCTTC 5 cut(s) 64, 76, 1833, 1973, 2333
Ecl136II GAGCTC 2 cut(s) 2242, 2534
Eco105I TACGTA 1 cut(s) 2482
Eco130I CCWWGG 2 cut(s) 15, 2110
Eco147I AGGCCT 1 cut(s) 297
Eco24I GRGCYC 2 cut(s) 2244, 2536
Eco31I GGTCTC 2 cut(s) 689, 1787
Eco47I GGWCC 2 cut(s) 155, 2731
Eco47III AGCGCT 3 cut(s) 1480, 2172, 2505
Eco53kI GAGCTC 2 cut(s) 2242, 2534
Eco57I CTGAAG 2 cut(s) 1416, 1503
EcoICRI GAGCTC 2 cut(s) 2242, 2534
EcoO109I RGGNCCY 1 cut(s) 2731
EcoRI GAATTC 1 cut(s) 1390
EcoRII CCWGG 2 cut(s) 927, 1255
EcoT14I CCWWGG 2 cut(s) 15, 2110
EcoT22I ATGCAT 2 cut(s) 799, 2518
EcoT38I GRGCYC 2 cut(s) 2244, 2536
ErhI CCWWGG 2 cut(s) 15, 2110
Esp3I CGTCTC 1 cut(s) 138
FalI AAGNNNNNCTT 2 cut(s) 2252, 2284
FaqI GGGAC 2 cut(s) 1409, 2717
FbaI TGATCA 1 cut(s) 300
FblI GTMKAC 1 cut(s) 131
FokI GGATG 7 cut(s) 1022, 1244, 1912, 1916, 2136, 2535, 2627
FriOI GRGCYC 2 cut(s) 2244, 2536
FspBI CTAG 4 cut(s) 567, 654, 861, 1080
GlaI GCGC 6 cut(s) 150, 1261, 1480, 1959, 2172, 2505
GsuI CTGGAG 1 cut(s) 831
HaeII RGCGCY 3 cut(s) 1482, 2174, 2507
HaeIII GGCC 2 cut(s) 297, 1688
HapII CCGG 5 cut(s) 453, 711, 1570, 2118, 2391
HgaI GACGC 4 cut(s) 155, 507, 846, 1072
HhaI GCGC 6 cut(s) 151, 1262, 1481, 1960, 2173, 2506
Hin6I GCGC 6 cut(s) 149, 1260, 1479, 1958, 2171, 2504
HinP1I GCGC 6 cut(s) 149, 1260, 1479, 1958, 2171, 2504
HincII GTYRAC 1 cut(s) 1741
HindII GTYRAC 1 cut(s) 1741
HindIII AAGCTT 3 cut(s) 305, 458, 887
HinfI GANTC 8 cut(s) 11, 924, 1190, 1246, 1252, 1496, 1715, 2263
HpaII CCGG 5 cut(s) 453, 711, 1570, 2118, 2391
HphI GGTGA 3 cut(s) 1100, 2167, 2729
Hpy166II GTNNAC 7 cut(s) 132, 672, 1275, 1294, 1342, 1741, 2079
Hpy188I TCNGA 9 cut(s) 330, 607, 678, 694, 1139, 1251, 1396, 1579, 1822
Hpy188III TCNNGA 8 cut(s) 268, 387, 547, 883, 1226, 1426, 1508, 1988
Hpy8I GTNNAC 7 cut(s) 132, 672, 1275, 1294, 1342, 1741, 2079
Hpy99I CGWCG 1 cut(s) 1088
HpyCH4III ACNGT 5 cut(s) 110, 1145, 1183, 1291, 2408
HpyCH4IV ACGT 4 cut(s) 210, 968, 1214, 2481
HpyF10VI GCNNNNNNNGC 7 cut(s) 157, 1662, 1671, 1803, 2094, 2179, 2585
HpyF3I CTNAG 7 cut(s) 215, 221, 586, 693, 1386, 1509, 1855
HpySE526I ACGT 4 cut(s) 210, 968, 1214, 2481
HspAI GCGC 6 cut(s) 149, 1260, 1479, 1958, 2171, 2504
Ksp22I TGATCA 1 cut(s) 300
LguI GCTCTTC 1 cut(s) 2333
LmnI GCTCC 3 cut(s) 1170, 1510, 2112
LweI GCATC 8 cut(s) 325, 520, 604, 636, 1000, 1570, 1572, 2406
MaeI CTAG 4 cut(s) 567, 654, 861, 1080
MaeII ACGT 4 cut(s) 210, 968, 1214, 2481
MaeIII GTNAC 6 cut(s) 182, 206, 421, 441, 1183, 1196
MflI RGATCY 2 cut(s) 1348, 1635
MhlI GDGCHC 6 cut(s) 1307, 2109, 2244, 2358, 2433, 2536
MlyI GAGTC 5 cut(s) 933, 1199, 1240, 1709, 2257
MmeI TCCRAC 1 cut(s) 1845
Mph1103I ATGCAT 2 cut(s) 799, 2518
MroXI GAANNNNTTC 1 cut(s) 358
MseI TTAA 5 cut(s) 531, 1607, 1850, 2130, 2136
MslI CAYNNNNRTG 1 cut(s) 2015
MspA1I CMGCKG 1 cut(s) 1124
MspCI CTTAAG 1 cut(s) 530
MspI CCGG 5 cut(s) 453, 711, 1570, 2118, 2391
MspR9I CCNGG 3 cut(s) 454, 929, 1257
MvaI CCWGG 2 cut(s) 929, 1257
MvnI CGCG 5 cut(s) 149, 548, 1262, 1346, 1960
MwoI GCNNNNNNNGC 7 cut(s) 157, 1662, 1671, 1803, 2094, 2179, 2585
NciI CCSGG 1 cut(s) 454
NcoI CCATGG 1 cut(s) 15
NlaIV GGNNCC 4 cut(s) 583, 1350, 2116, 2733
NmeAIII GCCGAG 2 cut(s) 1851, 2377
NmuCI GTSAC 3 cut(s) 206, 441, 1183
NruI TCGCGA 1 cut(s) 548
NsiI ATGCAT 2 cut(s) 799, 2518
NspI RCATGY 2 cut(s) 1044, 2516
NspV TTCGAA 2 cut(s) 627, 1218
PceI AGGCCT 1 cut(s) 297
PciSI GCTCTTC 1 cut(s) 2333
PdmI GAANNNNTTC 1 cut(s) 358
PfeI GAWTC 3 cut(s) 11, 1252, 1496
PflFI GACNNNGTC 1 cut(s) 1189
PflMI CCANNNNNTGG 1 cut(s) 2610
PfoI TCCNGGA 1 cut(s) 452
PleI GAGTC 5 cut(s) 932, 1198, 1240, 1709, 2257
PpsI GAGTC 5 cut(s) 932, 1198, 1240, 1709, 2257
Ppu21I YACGTR 1 cut(s) 2482
PpuMI RGGWCCY 1 cut(s) 2731
Psp124BI GAGCTC 2 cut(s) 2244, 2536
Psp1406I AACGTT 1 cut(s) 968
Psp5II RGGWCCY 1 cut(s) 2731
Psp6I CCWGG 2 cut(s) 927, 1255
PspGI CCWGG 2 cut(s) 927, 1255
PspN4I GGNNCC 4 cut(s) 583, 1350, 2116, 2733
PspPI GGNCC 2 cut(s) 155, 2731
PspPPI RGGWCCY 1 cut(s) 2731
PsrI GAACNNNNNNTAC 6 cut(s) 457, 489, 653, 685, 953, 985
PstI CTGCAG 4 cut(s) 67, 1679, 2199, 2590
PstNI CAGNNNCTG 2 cut(s) 854, 1508
PsuI RGATCY 2 cut(s) 1348, 1635
PsyI GACNNNGTC 1 cut(s) 1189
PvuII CAGCTG 1 cut(s) 1124
RruI TCGCGA 1 cut(s) 548
RsaI GTAC 8 cut(s) 358, 671, 706, 771, 1274, 1343, 1541, 2570
RsaNI GTAC 8 cut(s) 357, 670, 705, 770, 1273, 1342, 1540, 2569
RseI CAYNNNNRTG 1 cut(s) 2015
SacI GAGCTC 2 cut(s) 2244, 2536
SapI GCTCTTC 1 cut(s) 2333
SaqAI TTAA 5 cut(s) 531, 1607, 1850, 2130, 2136
Sau96I GGNCC 2 cut(s) 155, 2731
ScaI AGTACT 2 cut(s) 358, 706
SchI GAGTC 5 cut(s) 933, 1199, 1240, 1709, 2257
ScrFI CCNGG 3 cut(s) 454, 929, 1257
SduI GDGCHC 6 cut(s) 1307, 2109, 2244, 2358, 2433, 2536
SfaNI GCATC 8 cut(s) 325, 520, 604, 636, 1000, 1570, 1572, 2406
SfcI CTRYAG 6 cut(s) 63, 1500, 1675, 1719, 2195, 2586
SfuI TTCGAA 2 cut(s) 627, 1218
SinI GGWCC 2 cut(s) 155, 2731
SmiMI CAYNNNNRTG 1 cut(s) 2015
SmlI CTYRAG 3 cut(s) 530, 814, 1226
SmoI CTYRAG 3 cut(s) 530, 814, 1226
SnaBI TACGTA 1 cut(s) 2482
SpeI ACTAGT 1 cut(s) 566
SseBI AGGCCT 1 cut(s) 297
SsiI CCGC 6 cut(s) 158, 172, 198, 579, 1346, 2095
SspI AATATT 1 cut(s) 1546
SspMI CTAG 4 cut(s) 567, 654, 861, 1080
SstI GAGCTC 2 cut(s) 2244, 2536
StuI AGGCCT 1 cut(s) 297
StyD4I CCNGG 3 cut(s) 452, 927, 1255
StyI CCWWGG 2 cut(s) 15, 2110
TaaI ACNGT 5 cut(s) 110, 1145, 1183, 1291, 2408
TaiI ACGT 4 cut(s) 213, 971, 1217, 2484
TaqI TCGA 8 cut(s) 81, 388, 627, 964, 972, 1218, 1469, 2500
TatI WGTACW 5 cut(s) 356, 669, 704, 769, 1272
TfiI GAWTC 3 cut(s) 11, 1252, 1496
Tru1I TTAA 5 cut(s) 531, 1607, 1850, 2130, 2136
Tru9I TTAA 5 cut(s) 531, 1607, 1850, 2130, 2136
TscAI CASTG 3 cut(s) 113, 211, 993
TseFI GTSAC 3 cut(s) 206, 441, 1183
Tsp45I GTSAC 3 cut(s) 206, 441, 1183
TspGWI ACGGA 2 cut(s) 1341, 2177
TspRI CASTG 3 cut(s) 113, 211, 993
Tth111I GACNNNGTC 1 cut(s) 1189
Van91I CCANNNNNTGG 1 cut(s) 2610
Vha464I CTTAAG 1 cut(s) 530
VpaK11BI GGWCC 2 cut(s) 155, 2731
XapI RAATTY 3 cut(s) 77, 935, 1390
XceI RCATGY 2 cut(s) 1044, 2516
XcmI CCANNNNNNNNNTGG 3 cut(s) 13, 985, 1897
XmiI GTMKAC 1 cut(s) 131
XmnI GAANNNNTTC 1 cut(s) 358
XspI CTAG 4 cut(s) 567, 654, 861, 1080
ZrmI AGTACT 2 cut(s) 358, 706
Zsp2I ATGCAT 2 cut(s) 799, 2518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.