Prupe.4G047300_v2.0.a1

Protein of unknown function (DUF2921)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000420
Physical Location & Seq
Reverse (-)
2249105 .. 2251987
2883 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G047300.1

Sequence Viewer

Length: 2883 bp
ATGGAGTCCAATTCTTCATCATCTTCTTCGGCTCCATTGCATAAATCTAGGGTTAGGATTCATACAATTTCGCCAACGAGGTCACTCCTCTTATTCTTCTTCCTTTTCTTCTGCATCAATTTCACCACCACTGCCTCAAACTCCATAACCCAAAGCTCAGATTCTGAAACCTTATACTCCAAACACTGCAACGACGTCGTGCCGAAGTCGGACACAGACCCCACCAGGTGGTTTGTCACCAACCTCTCGATCCAAGACATTGGCTTCAGGAACGGTTACTTCACCGGCGGCGATCAGCTTTTCAAGCAAAACCTGAAAACTAGCGAGGTCGATGACCTGAAAGCTCTGTCCTTTGTGCCCTCTGATGTGGGTAAAACCCTCACCGAAAGCGTTTTCAAAGTCAGGGCAACCTTGCATCTTCGAGACTACTCCATCTTCCACAATTCTACGCGTCGGAATTTGCGGTTGGTTTATTTCAAAGGTCCGAGAAGCCATTTTCGGAAGGGCTTACTGAACTTTAGGCTTGATGGGTACTACTCAGAATCTTCTAAGAAGCTTTGTATGGTTGGAAATGGCGGGTCAGGTAATTTGAGGCCTCTTAGTGTTGTTCTTAAGCTTAATTATCCGCGAAGTTCAAGTATTTATGACAGTTTAATTACTGGGACTTTAGAGAGTTTGAGTGATAAGCATGATGGGGATTATTTTGAACCCCTTTTGATGTTGGGTTTATATCAGAGTTCTAGTTATGAATATAAATTAGCTGGGAAAGATAGTGAGAATGGTTGTTTGCGTGGTGACGATAGAGGAGAGAATTTAGGTGTGGGCAAATCGAAGCGTGGTTTGTGTATGTTGCTTGGTAAACTACATGAAAGCTTTGAATTGGAATATGGAAGTGATTGTGGCAGTGTGAATTGCAATCCTCTTGGTGGGAATGCTGGATATGTATCTTCTTTTGTTTATTATGGAACTCGGTGTGCAGATGGGAGGAAAATGCAGATGTTGTTGGGTTTTCCCAACTCTAGCTATTATGGCATCAAATTTCCGTTTGACCCTCACACAACCTTCATTACTGAAGGGGCATGGGATGAGAAGGAGAATCGTCTGTGTGCTGTCGCATGCCGGATTTTGAATTTTACAGAATCTTTGACTTATGCTTTTGTTGGAGATTGCTCAACTAAATTCAGCTTGAGATTGCCAACAAAGTTGTCTTTATGGAATAGGTCTACTGTTGTGGGGGAAATGTGGAGCATCAAAGAAGTGAATGACTCAGGTTACTTTGCTAAAATTGGGTTTCACACTTTGTCGGGATGGTTGATGAAACTCTTAGATTTCAAATATGAGTATTCTGAGAATGATGATATGAGAAAAACATGTGCAGAAAAGAAGGCTGGGAGAGGCAAAGGAAAGATATACCCTGATGAGTTTTCAGTGGACATGAAATTTGGCATGACAGTGAGAAACAGTAAAGGACAACAAGCATCTGGTTATTCATCCCCTTTATTTGTGGAGGATGAGCGGGTTTATGGACGCCGGTTTTGGGATAAATTGCCGCAAACAGAATCATCAATGCAACTGAACCAAAGCCATACCCATAGCAGTCCGATGAACGTCAGCTATAAGTTGTTCTTCATATCTGATTTCGGTTTTCGTCATGATGTTTTCCCATCTAAAGCAGAACTATCTGCTGAAGGAATATATGATAGGGATTATGGTAATCTGTGCATGATAGGATGTAGGCATGTACCAGTGAAAAACAAAACATTGATTAAACAGGATATGTTAGACTGTGCTATTAAAATCATTGTACATTTTTCTCCATTGGATACGAAGGATGGCCAGAATGTCAAGGGAACTATTGAAAGCACAAGGGGAAAGTTAGACCCTCTTTATTTTGAACCTATTGAGTTTTCTTCGAATTCTATTTACACTAGCCAAGCTGCAGCATCCATTTCAAGAATCGACTTTGAGATCAGTATGGTTCTGATTTCCAACACCCTTGCATGTGTCTTTGTGGGCTTGCAGCTATTATTTGTGAAGAAGCACCCAGATGTTCTCCCATTTGTTTCGATTGTAATGCTTATTGTTCTTAGTCTGGGATACATGATTCCACTTCTGGTCAACTTTGAAGCATTGTTTGTGCCAAATAAACACCACAGCCAGCAGGATACTTTTCTTGGGACTGGTGGATGGCTTCAAGTGAATGAAGTAGTAGTCAGGGTGTTAATGATGGTATCTCTTCTTTTGCAGCTACGTCTTCTCCAACTGACGTGGTCCTCAAGACAAGGTCATGGAAACCAGAAGAGCTTGAGAGATTCTGAGAGGAAGGTTCTTTATGCAACTTTACCATTGTACATAGCTGGTGCACTGATTGTTTGGTTTGTGAACCTTCGGAAAAACGCTTACCAGAGATCACACAGGCCATTTCAAAGACCACACCGCATGGCCTACCGAGTGTCTACTCTCCACCATCTTGCTTACCAGCAGCACTCTCTCCGGGAGGATCTCAGCTCTTATGCCGGCCTCGTACTGGATAGTTTTTTGCTCCCACAGATATTGTTCAACTTATTCTTAAATTCAGGAGAAAAGACTCTGGCGTGCGCATTTTACCTTGGAACCACCGTAATTCGTCTGCTGCCTCATGCATATGATCTTTACAGAGCTCAGACTGGTACATGGTTCCTTGACTTATCGTATATTTATGCAAACCATAAAATGGATTTTTACTCAACTGCCTGGAATATCATCATCCCCTGTGGTGGTCTGCTGTTTGCTGCCATTATTTTCTTGCAGCAGAGATTTGGTGGTCGTTTCATTCTTCCCAAAAGATTTAGTTTGACTTCTGTATATGAGAAAGTCCCTGTCATCAGCAATGAGGAATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
DUF2921

Protein Analysis

961

Amino Acids

108.84

Weight (kDa)

8.96

Isoelectric Point (pI)

39.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21700
fragaria_vesca FvH4_1g10360 FvH4_1g10760 FvH4_2g30370 FvH4_3g09240 FvH4_3g20050 FvH4_3g20280 FvH4_3g20290 FvH4_3g20291 FvH4_3g20340 FvH4_3g20350 FvH4_3g20351 FvH4_3g20380 FvH4_3g20390 FvH4_5g13190 FvH4_6g20070 FvH4_6g42890
malus_domestica MD03G1227000.v1.1 MD10G1294900.v1.1 MD11G1246300.v1.1 MD11G1246400.v1.1 MD11G1246500.v1.1 MD11G1246600.v1.1
prunus_persica Prupe.4G047300_v2.0.a1 Prupe.4G181700_v2.0.a1 Prupe.4G181900_v2.0.a1 Prupe.4G182000_v2.0.a1 Prupe.4G182200_v2.0.a1
pyrus_communis pycom03g17470 pycom10g24670 pycom11g21640
rosa_chinensis RchiOBHm_Chr5g0008381 RchiOBHm_Chr5g0008391 RchiOBHm_Chr5g0033881 RchiOBHm_Chr5g0034171 RchiOBHm_Chr5g0034181 RchiOBHm_Chr5g0034191 RchiOBHm_Chr5g0034201 RchiOBHm_Chr5g0034211 RchiOBHm_Chr5g0034221 RchiOBHm_Chr5g0034231 RchiOBHm_Chr5g0034271 RchiOBHm_Chr5g0034281
rosa_laevigata RLG00000031566 RLG00000031567 RLG00000033506 RLG00000033529 RLG00000033530 RLG00000033531
rosa_multiflora Rmu_co8445509.1_g000001 Rmu_co8470615.1_g000001 Rmu_sc0008805.1_g000003 Rmu_sc0008805.1_g000004 Rmu_ssc0000128.1_g000029 Rmu_ssc0000128.1_g000031 Rmu_ssc0000128.1_g000032 Rmu_ssc0000388.1_g000062 Rmu_ssc0000388.1_g000063
rosa_roxburghii Rroxscaffold_1G00046010 Rroxscaffold_1G00046020 Rroxscaffold_1G00046030 Rroxscaffold_1G00046040 Rroxscaffold_1G00046060 Rroxscaffold_1G00046070 Rroxscaffold_1G00046080 Rroxscaffold_1G00046090
rosa_rugosa Rorug04G0435500 Rorug05G0142900 Rorug05G0144600 Rorug05G0144600 Rorug05G0144700 Rorug05G0144800
rosa_samantha Rh5AG233800 Rh5AG235900 Rh5AG236000 Rh5AG236100 Rh5AG236200 Rh5AG236300 Rh5AG236500 Rh5BG061200 Rh5BG061300 Rh5BG234300 Rh5BG236600 Rh5BG236800 Rh5CG071200 Rh5DG060000 Rh5DG060100 Rh5DG241300 Rh5DG243700 Rh5DG244000 Rh5DG244100
rosa_wichuraiana Rw3G018950 Rw5G005680 Rw5G021330 Rw5G021340 Rw5G021350 Rw5G021550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 198
Acc16I TGCGCA 1 cut(s) 2599
AccB7I CCANNNNNTGG 2 cut(s) 228, 2713
AccBSI CCGCTC 1 cut(s) 1516
AccI GTMKAC 2 cut(s) 1223, 2456
AccII CGCG 2 cut(s) 451, 628
AciI CCGC 7 cut(s) 288, 463, 576, 626, 1516, 1550, 2437
AclWI GGATC 2 cut(s) 244, 2508
AcoI YGGCCR 1 cut(s) 1834
AcsI RAATTY 8 cut(s) 457, 811, 1037, 1129, 1178, 1439, 1915, 2572
AcuI CTGAAG 3 cut(s) 250, 1092, 1707
AcyI GRCGYC 2 cut(s) 195, 1528
AdeI CACNNNGTG 1 cut(s) 228
AfaI GTAC 6 cut(s) 533, 1743, 1806, 2351, 2526, 2671
AfiI CCNNNNNNNGG 6 cut(s) 228, 926, 1537, 2527, 2713, 2756
AflII CTTAAG 1 cut(s) 611
AflIII ACRYGT 2 cut(s) 449, 1370
AjiI CACGTC 1 cut(s) 2268
AjnI CCWGG 2 cut(s) 224, 2732
AjuI GAANNNNNNNTTGG 2 cut(s) 449, 481
Alw21I GWGCWC 2 cut(s) 2365, 2662
Alw26I GTCTC 1 cut(s) 417
Alw44I GTGCAC 1 cut(s) 2361
AlwI GGATC 2 cut(s) 244, 2508
AlwNI CAGNNNCTG 1 cut(s) 164
AoxI GGCC 5 cut(s) 593, 1834, 2417, 2442, 2518
ApaLI GTGCAC 1 cut(s) 2361
ApeKI GCWGC 8 cut(s) 1937, 1940, 2020, 2245, 2482, 2632, 2771, 2788
ApoI RAATTY 8 cut(s) 457, 811, 1037, 1129, 1178, 1439, 1915, 2572
ArsI GACNNNNNNTTYG 4 cut(s) 1424, 1456, 1518, 1550
AspLEI GCGC 1 cut(s) 2600
AspS9I GGNCC 2 cut(s) 482, 2271
AsuC2I CCSGG 1 cut(s) 2495
AsuHPI GGTGA 5 cut(s) 115, 229, 274, 373, 806
AsuII TTCGAA 1 cut(s) 1913
AvaII GGWCC 2 cut(s) 482, 2271
BaeGI GKGCMC 2 cut(s) 360, 2365
BalI TGGCCA 1 cut(s) 1836
BanII GRGCYC 1 cut(s) 2662
BbsI GAAGAC 1 cut(s) 2246
Bbv12I GWGCWC 2 cut(s) 2365, 2662
BbvI GCAGC 8 cut(s) 1924, 1952, 2032, 2257, 2494, 2619, 2758, 2800
BcgI CGANNNNNNTGC 4 cut(s) 178, 212, 2056, 2090
BciT130I CCWGG 2 cut(s) 226, 2734
BciVI GTATCC 3 cut(s) 1816, 2090, 2158
BcnI CCSGG 1 cut(s) 2495
BcoDI GTCTC 1 cut(s) 417
BfaI CTAG 5 cut(s) 48, 321, 741, 1020, 1929
BfmI CTRYAG 1 cut(s) 1938
BfrI CTTAAG 1 cut(s) 611
BfuI GTATCC 3 cut(s) 1816, 2090, 2158
Bme1390I CCNGG 3 cut(s) 226, 2495, 2734
Bme18I GGWCC 2 cut(s) 482, 2271
BmgBI CACGTC 1 cut(s) 2268
BmgT120I GGNCC 2 cut(s) 482, 2271
BmiI GGNNCC 3 cut(s) 33, 2614, 2678
BmrFI CCNGG 3 cut(s) 226, 2495, 2734
BmrI ACTGGG 1 cut(s) 669
BmsI GCATC 6 cut(s) 123, 424, 1041, 1257, 1487, 1952
BmuI ACTGGG 1 cut(s) 669
BpiI GAAGAC 1 cut(s) 2246
Bpu14I TTCGAA 1 cut(s) 1913
BpuEI CTTGAG 3 cut(s) 1207, 2260, 2326
BpuMI CCSGG 1 cut(s) 2495
BsaBI GATNNNNATC 1 cut(s) 943
BsaHI GRCGYC 2 cut(s) 195, 1528
BsaJI CCNNGG 1 cut(s) 2608
BsaXI ACNNNNNCTCC 2 cut(s) 2241, 2271
Bsc4I CCNNNNNNNGG 6 cut(s) 228, 926, 1537, 2527, 2713, 2756
Bse118I RCCGGY 3 cut(s) 284, 1530, 2516
Bse1I ACTGG 5 cut(s) 664, 1745, 2185, 2532, 2671
Bse3DI GCAATG 2 cut(s) 35, 2875
Bse8I GATNNNNATC 1 cut(s) 943
BseBI CCWGG 2 cut(s) 226, 2734
BseDI CCNNGG 1 cut(s) 2608
BseGI GGATG 9 cut(s) 1090, 1313, 1490, 1516, 1736, 1837, 1943, 2192, 2745
BseJI GATNNNNATC 1 cut(s) 943
BseLI CCNNNNNNNGG 6 cut(s) 228, 926, 1537, 2527, 2713, 2756
BseMI GCAATG 2 cut(s) 35, 2875
BseMII CTCAG 7 cut(s) 171, 552, 1281, 1338, 2307, 2518, 2675
BseNI ACTGG 5 cut(s) 664, 1745, 2185, 2532, 2671
BseRI GAGGAG 2 cut(s) 77, 819
BseSI GKGCMC 2 cut(s) 360, 2365
BseXI GCAGC 8 cut(s) 1924, 1952, 2032, 2257, 2494, 2619, 2758, 2800
BseYI CCCAGC 2 cut(s) 761, 1388
BsgI GTGCAG 2 cut(s) 996, 1395
Bsh1236I CGCG 2 cut(s) 451, 628
BshFI GGCC 5 cut(s) 595, 1836, 2419, 2444, 2520
BsiHKAI GWGCWC 2 cut(s) 2365, 2662
BsiSI CCGG 5 cut(s) 285, 1120, 1531, 2494, 2517
BslFI GGGAC 3 cut(s) 676, 2191, 2840
BslI CCNNNNNNNGG 6 cut(s) 228, 926, 1537, 2527, 2713, 2756
BsmAI GTCTC 1 cut(s) 417
BsmFI GGGAC 3 cut(s) 676, 2191, 2840
BsmI GAATGC 1 cut(s) 937
BsnI GGCC 5 cut(s) 595, 1836, 2419, 2444, 2520
Bsp119I TTCGAA 1 cut(s) 1913
Bsp1286I GDGCHC 3 cut(s) 360, 2365, 2662
Bsp1407I TGTACA 2 cut(s) 1804, 2349
Bsp143I GATC 6 cut(s) 249, 292, 1968, 2408, 2500, 2647
BspACI CCGC 7 cut(s) 288, 463, 576, 626, 1516, 1550, 2437
BspANI GGCC 5 cut(s) 595, 1836, 2419, 2444, 2520
BspCNI CTCAG 7 cut(s) 170, 551, 1280, 1339, 2308, 2517, 2674
BspFNI CGCG 2 cut(s) 451, 628
BspHI TCATGA 1 cut(s) 1651
BspLI GGNNCC 3 cut(s) 33, 2614, 2678
BspMAI CTGCAG 1 cut(s) 1942
BspPI GGATC 2 cut(s) 244, 2508
BspQI GCTCTTC 1 cut(s) 2294
BspT104I TTCGAA 1 cut(s) 1913
BspTI CTTAAG 1 cut(s) 611
BsrBI CCGCTC 1 cut(s) 1516
BsrDI GCAATG 2 cut(s) 35, 2875
BsrFI RCCGGY 3 cut(s) 284, 1530, 2516
BsrGI TGTACA 2 cut(s) 1804, 2349
BsrI ACTGG 5 cut(s) 664, 1745, 2185, 2532, 2671
BssAI RCCGGY 3 cut(s) 284, 1530, 2516
BssECI CCNNGG 1 cut(s) 2608
BssMI GATC 6 cut(s) 249, 292, 1968, 2408, 2500, 2647
BssNI GRCGYC 2 cut(s) 195, 1528
BssT1I CCWWGG 1 cut(s) 2608
Bst2UI CCWGG 2 cut(s) 226, 2734
Bst4CI ACNGT 7 cut(s) 275, 650, 1228, 1453, 1463, 1787, 2620
Bst6I CTCTTC 2 cut(s) 2241, 2294
BstACI GRCGYC 2 cut(s) 195, 1528
BstAFI CTTAAG 1 cut(s) 611
BstAUI TGTACA 2 cut(s) 1804, 2349
BstBI TTCGAA 1 cut(s) 1913
BstC8I GCNNGC 5 cut(s) 1117, 2018, 2159, 2518, 2596
BstF5I GGATG 9 cut(s) 1090, 1313, 1490, 1516, 1736, 1837, 1943, 2192, 2745
BstFNI CGCG 2 cut(s) 451, 628
BstHHI GCGC 1 cut(s) 2600
BstKTI GATC 6 cut(s) 252, 295, 1971, 2411, 2503, 2650
BstMAI GTCTC 1 cut(s) 417
BstMBI GATC 6 cut(s) 249, 292, 1968, 2408, 2500, 2647
BstMWI GCNNNNNNNGC 2 cut(s) 304, 1029
BstNI CCWGG 2 cut(s) 226, 2734
BstNSI RCATGY 4 cut(s) 1119, 1374, 1742, 2004
BstSCI CCNGG 3 cut(s) 224, 2493, 2732
BstSFI CTRYAG 1 cut(s) 1938
BstSLI GKGCMC 2 cut(s) 360, 2365
BstUI CGCG 2 cut(s) 451, 628
BstV1I GCAGC 8 cut(s) 1924, 1952, 2032, 2257, 2494, 2619, 2758, 2800
BstV2I GAAGAC 1 cut(s) 2246
BstX2I RGATCY 1 cut(s) 2500
BstXI CCANNNNNNTGG 1 cut(s) 260
BstYI RGATCY 1 cut(s) 2500
BsuI GTATCC 3 cut(s) 1816, 2090, 2158
BsuRI GGCC 5 cut(s) 595, 1836, 2419, 2444, 2520
BtrI CACGTC 1 cut(s) 2268
BtsCI GGATG 9 cut(s) 1090, 1313, 1490, 1516, 1736, 1837, 1943, 2192, 2745
BtsI GCAGTG 3 cut(s) 129, 184, 910
BtsIMutI CAGTG 7 cut(s) 129, 184, 910, 1434, 1458, 1752, 2363
Cac8I GCNNGC 5 cut(s) 1117, 2018, 2159, 2518, 2596
CaiI CAGNNNCTG 1 cut(s) 164
CciI TCATGA 1 cut(s) 1651
CfoI GCGC 1 cut(s) 2600
Cfr10I RCCGGY 3 cut(s) 284, 1530, 2516
Cfr13I GGNCC 2 cut(s) 482, 2271
CseI GACGC 2 cut(s) 440, 1536
CsiI ACCWGGT 1 cut(s) 224
Csp6I GTAC 6 cut(s) 532, 1742, 1805, 2350, 2525, 2670
CspCI CAANNNNNGTGG 4 cut(s) 2249, 2284, 2535, 2570
CviQI GTAC 6 cut(s) 532, 1742, 1805, 2350, 2525, 2670
DpnI GATC 6 cut(s) 251, 294, 1970, 2410, 2502, 2649
DpnII GATC 6 cut(s) 249, 292, 1968, 2408, 2500, 2647
DraIII CACNNNGTG 1 cut(s) 228
EaeI YGGCCR 1 cut(s) 1834
Eam1104I CTCTTC 2 cut(s) 2241, 2294
EarI CTCTTC 2 cut(s) 2241, 2294
Ecl136II GAGCTC 1 cut(s) 2660
Eco130I CCWWGG 1 cut(s) 2608
Eco147I AGGCCT 1 cut(s) 595
Eco24I GRGCYC 1 cut(s) 2662
Eco47I GGWCC 2 cut(s) 482, 2271
Eco53kI GAGCTC 1 cut(s) 2660
Eco57I CTGAAG 3 cut(s) 250, 1092, 1707
EcoICRI GAGCTC 1 cut(s) 2660
EcoRI GAATTC 1 cut(s) 1915
EcoRII CCWGG 2 cut(s) 224, 2732
EcoT14I CCWWGG 1 cut(s) 2608
EcoT22I ATGCAT 1 cut(s) 2644
EcoT38I GRGCYC 1 cut(s) 2662
ErhI CCWWGG 1 cut(s) 2608
FalI AAGNNNNNCTT 2 cut(s) 1610, 1642
FaqI GGGAC 3 cut(s) 676, 2191, 2840
FauI CCCGC 2 cut(s) 569, 1509
FauNDI CATATG 1 cut(s) 2644
FblI GTMKAC 2 cut(s) 1223, 2456
FokI GGATG 9 cut(s) 1097, 1320, 1477, 1523, 1743, 1844, 1930, 2199, 2732
FriOI GRGCYC 1 cut(s) 2662
FspAI RTGCGCAY 1 cut(s) 2599
FspBI CTAG 5 cut(s) 48, 321, 741, 1020, 1929
FspI TGCGCA 1 cut(s) 2599
GlaI GCGC 1 cut(s) 2599
GsaI CCCAGC 2 cut(s) 765, 1392
HaeIII GGCC 5 cut(s) 595, 1836, 2419, 2444, 2520
HapII CCGG 5 cut(s) 285, 1120, 1531, 2494, 2517
HgaI GACGC 2 cut(s) 440, 1536
HhaI GCGC 1 cut(s) 2600
Hin1I GRCGYC 2 cut(s) 195, 1528
Hin6I GCGC 1 cut(s) 2598
HinP1I GCGC 1 cut(s) 2598
HincII GTYRAC 1 cut(s) 2119
HindII GTYRAC 1 cut(s) 2119
HindIII AAGCTT 3 cut(s) 554, 614, 871
HpaII CCGG 5 cut(s) 285, 1120, 1531, 2494, 2517
HphI GGTGA 5 cut(s) 115, 229, 274, 373, 806
Hpy166II GTNNAC 7 cut(s) 860, 1224, 1432, 2119, 2363, 2383, 2457
Hpy188III TCNNGA 8 cut(s) 247, 268, 422, 1305, 1652, 1953, 2277, 2577
Hpy8I GTNNAC 7 cut(s) 860, 1224, 1432, 2119, 2363, 2383, 2457
Hpy99I CGWCG 3 cut(s) 197, 200, 456
HpyAV CCTTC 9 cut(s) 496, 1067, 1072, 1084, 1378, 1682, 1822, 2317, 2396
HpyCH4III ACNGT 7 cut(s) 275, 650, 1228, 1453, 1463, 1787, 2620
HpyCH4IV ACGT 4 cut(s) 195, 1608, 2251, 2267
HpyF10VI GCNNNNNNNGC 2 cut(s) 304, 1029
HpySE526I ACGT 4 cut(s) 195, 1608, 2251, 2267
Hsp92I GRCGYC 2 cut(s) 195, 1528
HspAI GCGC 1 cut(s) 2598
KroI GCCGGC 1 cut(s) 2516
KroNI GCCGGC 1 cut(s) 2518
Kzo9I GATC 6 cut(s) 249, 292, 1968, 2408, 2500, 2647
LguI GCTCTTC 1 cut(s) 2294
LmnI GCTCC 3 cut(s) 37, 1245, 2547
Lsp1109I GCAGC 8 cut(s) 1924, 1952, 2032, 2257, 2494, 2619, 2758, 2800
LweI GCATC 6 cut(s) 123, 424, 1041, 1257, 1487, 1952
MabI ACCWGGT 1 cut(s) 224
MaeI CTAG 5 cut(s) 48, 321, 741, 1020, 1929
MaeII ACGT 4 cut(s) 195, 1608, 2251, 2267
MaeIII GTNAC 5 cut(s) 81, 235, 275, 794, 1271
MalI GATC 6 cut(s) 251, 294, 1970, 2410, 2502, 2649
MbiI CCGCTC 1 cut(s) 1516
MboI GATC 6 cut(s) 249, 292, 1968, 2408, 2500, 2647
MflI RGATCY 1 cut(s) 2500
MhlI GDGCHC 3 cut(s) 360, 2365, 2662
MlsI TGGCCA 1 cut(s) 1836
MluI ACGCGT 1 cut(s) 449
MluNI TGGCCA 1 cut(s) 1836
MlyI GAGTC 3 cut(s) 14, 1259, 2581
MmeI TCCRAC 6 cut(s) 189, 434, 547, 1141, 2013, 2284
Mox20I TGGCCA 1 cut(s) 1836
Mph1103I ATGCAT 1 cut(s) 2644
MroNI GCCGGC 1 cut(s) 2516
MscI TGGCCA 1 cut(s) 1836
MseI TTAA 7 cut(s) 612, 618, 653, 1767, 1794, 2222, 2570
MslI CAYNNNNRTG 3 cut(s) 1451, 2046, 2643
Msp20I TGGCCA 1 cut(s) 1836
MspCI CTTAAG 1 cut(s) 611
MspI CCGG 5 cut(s) 285, 1120, 1531, 2494, 2517
MspR9I CCNGG 3 cut(s) 226, 2495, 2734
Mva1269I GAATGC 1 cut(s) 937
MvaI CCWGG 2 cut(s) 226, 2734
MvnI CGCG 2 cut(s) 451, 628
MwoI GCNNNNNNNGC 2 cut(s) 304, 1029
NaeI GCCGGC 1 cut(s) 2518
NciI CCSGG 1 cut(s) 2495
NdeI CATATG 1 cut(s) 2644
NdeII GATC 6 cut(s) 249, 292, 1968, 2408, 2500, 2647
NgoMIV GCCGGC 1 cut(s) 2516
NlaIV GGNNCC 3 cut(s) 33, 2614, 2678
NmuCI GTSAC 3 cut(s) 81, 235, 794
NsbI TGCGCA 1 cut(s) 2599
NsiI ATGCAT 1 cut(s) 2644
NspI RCATGY 4 cut(s) 1119, 1374, 1742, 2004
NspV TTCGAA 1 cut(s) 1913
PaeI GCATGC 1 cut(s) 1119
PagI TCATGA 1 cut(s) 1651
PceI AGGCCT 1 cut(s) 595
PciI ACATGT 1 cut(s) 1370
PciSI GCTCTTC 1 cut(s) 2294
PctI GAATGC 1 cut(s) 937
PdiI GCCGGC 1 cut(s) 2518
PfeI GAWTC 9 cut(s) 58, 161, 542, 1096, 1139, 1559, 1956, 2104, 2312
PflFI GACNNNGTC 2 cut(s) 2269, 2283
PflMI CCANNNNNTGG 2 cut(s) 228, 2713
PfoI TCCNGGA 1 cut(s) 2493
PleI GAGTC 3 cut(s) 13, 1259, 2581
PpsI GAGTC 3 cut(s) 13, 1259, 2581
PscI ACATGT 1 cut(s) 1370
Psp124BI GAGCTC 1 cut(s) 2662
Psp6I CCWGG 2 cut(s) 224, 2732
PspFI CCCAGC 2 cut(s) 761, 1388
PspGI CCWGG 2 cut(s) 224, 2732
PspN4I GGNNCC 3 cut(s) 33, 2614, 2678
PspPI GGNCC 2 cut(s) 482, 2271
PstI CTGCAG 1 cut(s) 1942
PstNI CAGNNNCTG 1 cut(s) 164
PsuI RGATCY 1 cut(s) 2500
PsyI GACNNNGTC 2 cut(s) 2269, 2283
RsaI GTAC 6 cut(s) 533, 1743, 1806, 2351, 2526, 2671
RsaNI GTAC 6 cut(s) 532, 1742, 1805, 2350, 2525, 2670
RseI CAYNNNNRTG 3 cut(s) 1451, 2046, 2643
SacI GAGCTC 1 cut(s) 2662
SapI GCTCTTC 1 cut(s) 2294
SaqAI TTAA 7 cut(s) 612, 618, 653, 1767, 1794, 2222, 2570
Sau3AI GATC 6 cut(s) 249, 292, 1968, 2408, 2500, 2647
Sau96I GGNCC 2 cut(s) 482, 2271
SchI GAGTC 3 cut(s) 14, 1259, 2581
ScrFI CCNGG 3 cut(s) 226, 2495, 2734
SduI GDGCHC 3 cut(s) 360, 2365, 2662
SexAI ACCWGGT 1 cut(s) 224
SfaNI GCATC 6 cut(s) 123, 424, 1041, 1257, 1487, 1952
SfcI CTRYAG 1 cut(s) 1938
SfuI TTCGAA 1 cut(s) 1913
SgrAI CRCCGGYG 1 cut(s) 284
SinI GGWCC 2 cut(s) 482, 2271
SmiMI CAYNNNNRTG 3 cut(s) 1451, 2046, 2643
SmlI CTYRAG 4 cut(s) 611, 1186, 2275, 2305
SmoI CTYRAG 4 cut(s) 611, 1186, 2275, 2305
SphI GCATGC 1 cut(s) 1119
SseBI AGGCCT 1 cut(s) 595
SsiI CCGC 7 cut(s) 288, 463, 576, 626, 1516, 1550, 2437
SspMI CTAG 5 cut(s) 48, 321, 741, 1020, 1929
SstI GAGCTC 1 cut(s) 2662
StuI AGGCCT 1 cut(s) 595
StyD4I CCNGG 3 cut(s) 224, 2493, 2732
StyI CCWWGG 1 cut(s) 2608
TaaI ACNGT 7 cut(s) 275, 650, 1228, 1453, 1463, 1787, 2620
TaiI ACGT 4 cut(s) 198, 1611, 2254, 2270
TaqI TCGA 7 cut(s) 248, 330, 421, 830, 1913, 1959, 2066
TatI WGTACW 2 cut(s) 1804, 2349
TauI GCSGC 2 cut(s) 291, 1552
TfiI GAWTC 9 cut(s) 58, 161, 542, 1096, 1139, 1559, 1956, 2104, 2312
Tru1I TTAA 7 cut(s) 612, 618, 653, 1767, 1794, 2222, 2570
Tru9I TTAA 7 cut(s) 612, 618, 653, 1767, 1794, 2222, 2570
TscAI CASTG 7 cut(s) 136, 191, 910, 1434, 1458, 1752, 2370
TseFI GTSAC 3 cut(s) 81, 235, 794
TseI GCWGC 8 cut(s) 1937, 1940, 2020, 2245, 2482, 2632, 2771, 2788
Tsp45I GTSAC 3 cut(s) 81, 235, 794
TspGWI ACGGA 1 cut(s) 1032
TspRI CASTG 7 cut(s) 136, 191, 910, 1434, 1458, 1752, 2370
Tth111I GACNNNGTC 2 cut(s) 2269, 2283
Van91I CCANNNNNTGG 2 cut(s) 228, 2713
Vha464I CTTAAG 1 cut(s) 611
VneI GTGCAC 1 cut(s) 2361
VpaK11BI GGWCC 2 cut(s) 482, 2271
XapI RAATTY 8 cut(s) 457, 811, 1037, 1129, 1178, 1439, 1915, 2572
XceI RCATGY 4 cut(s) 1119, 1374, 1742, 2004
XmiI GTMKAC 2 cut(s) 1223, 2456
XspI CTAG 5 cut(s) 48, 321, 741, 1020, 1929
ZraI GACGTC 1 cut(s) 196
Zsp2I ATGCAT 1 cut(s) 2644
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.