MD11G1246300.v1.1

Protein of unknown function (DUF2921)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000420
Physical Location & Seq
Reverse (-)
35817673 .. 35820462
2790 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1246300.v1.1.491

Sequence Viewer

Length: 2790 bp
ATGACCAAAGAATCCATGGCCATCTCCATGATCATCTTTTTACCCAATCTTCTTATGTTCGTCTTTCTTTTCTTCACCATCTCTTCTGCAGCTTCCTCTAAATACGATTTCCAAACCACTTACGCTGATCACTGTGCTTCATTTGTTCCCCAGTCTTTACCGAAAGGTTTTGCGCTAGGACCGCCCTACCAACACCTCCTCACGGGTTACTACACCGGTGGCGGTGGCCTTCTTAGTCCTATCTCATCGTCTAATCATAGTGAAAAATCTGTTCAATTCTTTATCTTGAAGACTAGAGAAACGGATGTTCCAGGCCTGATCAAGATTCGAGGGACTTTATTGTTTCCAGGTGATACACATTACTTTGTGGGGAATTCTTCCACCACAAGTAACAGTTTCTCATCTGGATCAGTTCCTCGTCCAAGAAGATTAATACTTAAACTTGAAGGGTTCTGGTTACAATCTTCCGGGAAGATTTGTATGGTTGGTTCCGGTTATGGTTACTCAAAAGGACGAAGTTATTCGCTCAACATTCATCCTATTTTTAAGCTCTACAACTTCATGAATTCCACTAGTGTCACGAGTTTGAGTAGCGGAACTTTGGAGAACATGATCAGTTCCAAGAATGATCCAGATTATTTTGAACCTATCTCTATTTTGATGCTTCCCGGTATGAACTACCAGTACACTCTGATTTCAAATAGTTCTGAAAACAGAAATACTTCCGGTGGAAGTGATAATTCCAATCCCACAAGTTCCTTGAATATAAAGAGATTTTGTTCTGTACTCTCAAGAGAAGTGCGAAATCATGGATTTGATCTCAAATACTCAAGCTTTTGCTCTTCTGCCAAGAACTGCACTCCTTTGGCCACGTCTGATTTGGCTCGTGTTGTTTCGTTGAAGCTTATCGGGTGTCGGAATGATGCGAAAAGGTTGAGAGTTCTGGTCGAATTTGTAGACAGCAGCAACATCTGGTATCGAAGGCTATTCGATCCCAATACAACATTGATTGGAGAAGGCTCATGGGATGCAGAAAAAAATCAGCTGTCTTGTGTTGCGTGTCGATTCTTGGATGCAGCAGGTTCTTGGAACAATACTCGTGTCGGTGATTGTTCAACAAGAATTAACTTGCGGTTTTCTGCAGTATGGACAATCGGAAACACAAGTAGCATTGTGGGGGAGATTTGGAGCAACAAAAATGTGACAGAGTCGGGTTACTTTGAAAAGATCACGTTCCAAAGTCCTCAGGATGACATTGGAAGGGTTCTGATTCCTGGTGTGAAGTATGAGTACACCAAAACCAAACAAGTAAGTAATTTGTGCCCAAAAAAGAAGACTTCTCATCCCAAGATCAACGTGTACCCGAATCCGTTTTCTTATGACATGAGATTTGATGTGTCAGCTAAGAATTCTAAAAGACAAGCTGTGTGGGGAAGTTCTGTTCCGATTTCTGTTGGGAATCAATTTTATCAGGCGTATTGGTATTCGATGCAAGGTGTGATTGCAACCACCGAATCTGAATCTACAGCTCCTGAGAGCTCTCCTGTGAGATACAGCTTCAACCACAGCAATCCGTACAATATTAGCTACAAAATAAGCATCCACCTGCTATCTCATGCAAAGTTTCAAAATGCATCAGTTTTAAACGAAATGCAGATCTTTGCTGAAGGGATCTATGATGAAACCGAAGGCAGCCTGTGCATGGTAGGCTGCAGAAACCTGGCCTCAAAGGGTCAGCAGCCAACAAATGATTCTGTAGATTGCGGGATTGCTGTCAATTTTGAGTTCCCCCCAACAAATCCAACCAACAATTCGGGTTTCATCAAGGGCAGCATAAAAAGCACGCGAAAAAAGTCTGACCCTCTTTATTTTGAAACTTGGGATTTAACCTCAGCTTCCTTTACCCTCGCTGAAGAAAGGCGGTCCATTTGGAGGATGGATGTGGAGATCATATTGGTTCTTATATCCACCACGCTGGCATGCTTCTTTGTGGTGCGACAACTCTTCCATGTGAAAAAGTATCCAGATGTTCTTCCCTCCATTTCCATTTTCATGCTACTAATTCTAACTCTTGGCTACATGATACCCCTTATGTTAAACTTCAAAGCCATGTTCACGAGTAGCACCAACCACCAAAACGTGTTCCTTGGAACCGGAGGATGGCTTGAAGTTAATGAGGTAGTTGTGAGGGTGATAACAATGGTAGCTTTCTTGCTGCAAATCCGTCTTCTGCAGCTAACTTGGTCAGCAAGATCAGCACATGGAAATCGCGACGAACTCTGGCTCATGGAGAAGAAGACTCTCTTTGTGGCCTTACTACTATATGTTGCAGGGGCTTTCGCTGCTTGGCTTCTGCATGAACAGATTTGGAAAAAGAGCTACTACGCTGATCACTCAATTTTCAGCATTGGCTTCAAATCTTATGCCGGTTTGGTATTGGATGGTTTTTTGCTGCCTCAAGTTGTGCTCAACATGTTTTGCAAATCAAAAGAAAATGCTCTATCCATTTCGTTCTATGTTGGAACAACTTTTGTTCGAGTGGTGCCACATGCATACGATCTTTACAGGGCTCACAAATTTGCTCACCGTCAATTCAACGAGTCGTACCTGTATGCAAGCCCTGCAGCAGATTTTTACTCCACTTCTTGGGATGTCATAATTCCTTTTGGGGGTTTAGTGTTTGCTGGCGTCGTTTTCTTGCAGCAGAAGTTTGGGGGTCGTTGCATTCTCCCTCAAAAGTTGATAGAGTTGGGTGAATATGAGAAGGTCCCTACTGTTACTGAAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
DUF2921

Protein Analysis

930

Amino Acids

104.14

Weight (kDa)

8.99

Isoelectric Point (pI)

37.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2921_N PF25333 41 - 218 2.3e-16 DUF2921 lipocalin-like domain
DUF2921_N PF25333 244 - 411 1.7e-26 DUF2921 lipocalin-like domain
DUF2921_N PF25333 441 - 626 2.4e-35 DUF2921 lipocalin-like domain
DUF2921 PF11145 640 - 914 4e-65 Transmembrane E3 ligase/DUF2921 transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21700
fragaria_vesca FvH4_1g10360 FvH4_1g10760 FvH4_2g30370 FvH4_3g09240 FvH4_3g20050 FvH4_3g20280 FvH4_3g20290 FvH4_3g20291 FvH4_3g20340 FvH4_3g20350 FvH4_3g20351 FvH4_3g20380 FvH4_3g20390 FvH4_5g13190 FvH4_6g20070 FvH4_6g42890
malus_domestica MD03G1227000.v1.1 MD10G1294900.v1.1 MD11G1246300.v1.1 MD11G1246400.v1.1 MD11G1246500.v1.1 MD11G1246600.v1.1
prunus_persica Prupe.4G047300_v2.0.a1 Prupe.4G181700_v2.0.a1 Prupe.4G181900_v2.0.a1 Prupe.4G182000_v2.0.a1 Prupe.4G182200_v2.0.a1
pyrus_communis pycom03g17470 pycom10g24670 pycom11g21640
rosa_chinensis RchiOBHm_Chr5g0008381 RchiOBHm_Chr5g0008391 RchiOBHm_Chr5g0033881 RchiOBHm_Chr5g0034171 RchiOBHm_Chr5g0034181 RchiOBHm_Chr5g0034191 RchiOBHm_Chr5g0034201 RchiOBHm_Chr5g0034211 RchiOBHm_Chr5g0034221 RchiOBHm_Chr5g0034231 RchiOBHm_Chr5g0034271 RchiOBHm_Chr5g0034281
rosa_laevigata RLG00000031566 RLG00000031567 RLG00000033506 RLG00000033529 RLG00000033530 RLG00000033531
rosa_multiflora Rmu_co8445509.1_g000001 Rmu_co8470615.1_g000001 Rmu_sc0008805.1_g000003 Rmu_sc0008805.1_g000004 Rmu_ssc0000128.1_g000029 Rmu_ssc0000128.1_g000031 Rmu_ssc0000128.1_g000032 Rmu_ssc0000388.1_g000062 Rmu_ssc0000388.1_g000063
rosa_roxburghii Rroxscaffold_1G00046010 Rroxscaffold_1G00046020 Rroxscaffold_1G00046030 Rroxscaffold_1G00046040 Rroxscaffold_1G00046060 Rroxscaffold_1G00046070 Rroxscaffold_1G00046080 Rroxscaffold_1G00046090
rosa_rugosa Rorug04G0435500 Rorug05G0142900 Rorug05G0144600 Rorug05G0144600 Rorug05G0144700 Rorug05G0144800
rosa_samantha Rh5AG233800 Rh5AG235900 Rh5AG236000 Rh5AG236100 Rh5AG236200 Rh5AG236300 Rh5AG236500 Rh5BG061200 Rh5BG061300 Rh5BG234300 Rh5BG236600 Rh5BG236800 Rh5CG071200 Rh5DG060000 Rh5DG060100 Rh5DG241300 Rh5DG243700 Rh5DG244000 Rh5DG244100
rosa_wichuraiana Rw3G018950 Rw5G005680 Rw5G021330 Rw5G021340 Rw5G021350 Rw5G021550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1614
Acc36I ACCTGC 2 cut(s) 1070, 1614
AccB1I GGYRCC 1 cut(s) 2542
AccB7I CCANNNNNTGG 1 cut(s) 2646
AccI GTMKAC 1 cut(s) 957
AccII CGCG 2 cut(s) 1846, 2271
AciI CCGC 6 cut(s) 182, 222, 594, 1132, 1764, 1921
AclWI GGATC 4 cut(s) 415, 623, 986, 1679
AcoI YGGCCR 2 cut(s) 18, 867
AcsI RAATTY 5 cut(s) 373, 565, 950, 1408, 2576
AcuI CTGAAG 2 cut(s) 1686, 1932
AcyI GRCGYC 1 cut(s) 2688
AfaI GTAC 6 cut(s) 686, 786, 1292, 1361, 1577, 2606
AfiI CCNNNNNNNGG 6 cut(s) 202, 1702, 1932, 2160, 2646, 2669
AflIII ACRYGT 3 cut(s) 1356, 2139, 2472
AgeI ACCGGT 1 cut(s) 215
AhlI ACTAGT 1 cut(s) 572
AjiI CACGTC 1 cut(s) 873
AjnI CCWGG 4 cut(s) 310, 346, 1273, 1719
AjuI GAANNNNNNNTTGG 4 cut(s) 1735, 1767, 1795, 1827
AloI GAACNNNNNNTCC 2 cut(s) 1425, 1457
Alw21I GWGCWC 2 cut(s) 1541, 2469
AlwI GGATC 4 cut(s) 415, 623, 986, 1679
AlwNI CAGNNNCTG 1 cut(s) 1532
AoxI GGCC 6 cut(s) 18, 226, 313, 867, 1722, 2310
ApoI RAATTY 5 cut(s) 373, 565, 950, 1408, 2576
ArsI GACNNNNNNTTYG 2 cut(s) 1373, 1405
AseI ATTAAT 1 cut(s) 431
AsiGI ACCGGT 1 cut(s) 215
Asp700I GAANNNNTTC 2 cut(s) 520, 721
AspLEI GCGC 1 cut(s) 175
AspS9I GGNCC 3 cut(s) 179, 1923, 2767
AsuC2I CCSGG 2 cut(s) 469, 669
AsuHPI GGTGA 6 cut(s) 67, 362, 1118, 2203, 2576, 2765
AvaII GGWCC 3 cut(s) 179, 1923, 2767
AxyI CCTNAGG 1 cut(s) 1245
BaeGI GKGCMC 1 cut(s) 1325
BalI TGGCCA 2 cut(s) 20, 869
BanI GGYRCC 1 cut(s) 2542
BanII GRGCYC 2 cut(s) 1541, 2572
BarI GAAGNNNNNNTAC 2 cut(s) 1274, 1306
BauI CACGAG 4 cut(s) 580, 885, 1098, 2116
BbsI GAAGAC 4 cut(s) 296, 1340, 2219, 2303
Bbv12I GWGCWC 2 cut(s) 1541, 2469
BbvCI CCTCAGC 1 cut(s) 1891
BccI CCATC 5 cut(s) 29, 86, 1930, 2154, 2435
BciT130I CCWGG 4 cut(s) 312, 348, 1275, 1721
BciVI GTATCC 1 cut(s) 2031
BclI TGATCA 5 cut(s) 30, 127, 318, 612, 2389
BcnI CCSGG 2 cut(s) 469, 669
BcuI ACTAGT 1 cut(s) 572
BfaI CTAG 3 cut(s) 176, 294, 573
BfmI CTRYAG 7 cut(s) 87, 1140, 1524, 1711, 1755, 2231, 2622
BfuAI ACCTGC 2 cut(s) 1070, 1614
BfuI GTATCC 1 cut(s) 2031
BglII AGATCT 1 cut(s) 1656
Bme1390I CCNGG 6 cut(s) 312, 348, 469, 669, 1275, 1721
Bme18I GGWCC 3 cut(s) 179, 1923, 2767
BmgBI CACGTC 1 cut(s) 873
BmgT120I GGNCC 3 cut(s) 179, 1923, 2767
BmiI GGNNCC 4 cut(s) 490, 2152, 2544, 2769
BmrFI CCNGG 6 cut(s) 312, 348, 469, 669, 1275, 1721
BmrI ACTGGG 1 cut(s) 145
BmsI GCATC 7 cut(s) 651, 913, 1018, 1063, 1479, 1608, 1643
BmuI ACTGGG 1 cut(s) 145
BpiI GAAGAC 4 cut(s) 296, 1340, 2219, 2303
Bpu10I CCTNAGC 1 cut(s) 1891
BpuEI CTTGAG 3 cut(s) 775, 814, 2442
BpuMI CCSGG 2 cut(s) 469, 669
BsaHI GRCGYC 1 cut(s) 2688
BsaJI CCNNGG 2 cut(s) 15, 2146
BsaWI WCCGGW 4 cut(s) 215, 491, 725, 2153
Bsc4I CCNNNNNNNGG 6 cut(s) 202, 1702, 1932, 2160, 2646, 2669
Bse118I RCCGGY 2 cut(s) 215, 2426
Bse1I ACTGG 2 cut(s) 151, 682
Bse21I CCTNAGG 1 cut(s) 1245
BseBI CCWGG 4 cut(s) 312, 348, 1275, 1721
BseDI CCNNGG 2 cut(s) 15, 2146
BseLI CCNNNNNNNGG 6 cut(s) 202, 1702, 1932, 2160, 2646, 2669
BseMII CTCAG 3 cut(s) 1259, 1524, 1905
BseNI ACTGG 2 cut(s) 151, 682
BseRI GAGGAG 1 cut(s) 188
BseSI GKGCMC 1 cut(s) 1325
BsgI GTGCAG 1 cut(s) 841
Bsh1236I CGCG 2 cut(s) 1846, 2271
BshFI GGCC 6 cut(s) 20, 228, 315, 869, 1724, 2312
BshNI GGYRCC 1 cut(s) 2542
BshTI ACCGGT 1 cut(s) 215
BsiHKAI GWGCWC 2 cut(s) 1541, 2469
BsiSI CCGG 7 cut(s) 216, 468, 492, 669, 726, 2154, 2427
BslFI GGGAC 2 cut(s) 346, 2753
BslI CCNNNNNNNGG 6 cut(s) 202, 1702, 1932, 2160, 2646, 2669
BsmFI GGGAC 2 cut(s) 346, 2753
BsmI GAATGC 1 cut(s) 2724
BsnI GGCC 6 cut(s) 20, 228, 315, 869, 1724, 2312
Bsp1286I GDGCHC 4 cut(s) 1325, 1541, 2469, 2572
Bsp19I CCATGG 1 cut(s) 15
Bsp68I TCGCGA 1 cut(s) 2271
BspACI CCGC 6 cut(s) 182, 222, 594, 1132, 1764, 1921
BspANI GGCC 6 cut(s) 20, 228, 315, 869, 1724, 2312
BspCNI CTCAG 3 cut(s) 1258, 1525, 1904
BspFNI CGCG 2 cut(s) 1846, 2271
BspHI TCATGA 1 cut(s) 561
BspLI GGNNCC 4 cut(s) 490, 2152, 2544, 2769
BspMAI CTGCAG 5 cut(s) 91, 1144, 1715, 2235, 2626
BspMI ACCTGC 2 cut(s) 1070, 1614
BspPI GGATC 4 cut(s) 415, 623, 986, 1679
BspQI GCTCTTC 1 cut(s) 847
BspT107I GGYRCC 1 cut(s) 2542
BsrFI RCCGGY 2 cut(s) 215, 2426
BsrI ACTGG 2 cut(s) 151, 682
BssAI RCCGGY 2 cut(s) 215, 2426
BssECI CCNNGG 2 cut(s) 15, 2146
BssNI GRCGYC 1 cut(s) 2688
BssSI CACGAG 4 cut(s) 580, 885, 1098, 2116
BssT1I CCWWGG 2 cut(s) 15, 2146
Bst2BI CACGAG 4 cut(s) 580, 885, 1098, 2116
Bst2UI CCWGG 4 cut(s) 312, 348, 1275, 1721
Bst4CI ACNGT 4 cut(s) 134, 395, 2588, 2776
Bst6I CTCTTC 3 cut(s) 88, 847, 2009
BstACI GRCGYC 1 cut(s) 2688
BstAPI GCANNNNNTGC 2 cut(s) 1698, 2621
BstC8I GCNNGC 5 cut(s) 1844, 1977, 1981, 2617, 2686
BstDEI CTNAG 5 cut(s) 233, 1245, 1404, 1533, 1891
BstDSI CCRYGG 1 cut(s) 15
BstFNI CGCG 2 cut(s) 1846, 2271
BstHHI GCGC 1 cut(s) 175
BstMWI GCNNNNNNNGC 7 cut(s) 181, 1698, 1707, 1839, 2255, 2342, 2621
BstNI CCWGG 4 cut(s) 312, 348, 1275, 1721
BstNSI RCATGY 3 cut(s) 1983, 2476, 2552
BstSCI CCNGG 6 cut(s) 310, 346, 467, 667, 1273, 1719
BstSFI CTRYAG 7 cut(s) 87, 1140, 1524, 1711, 1755, 2231, 2622
BstSLI GKGCMC 1 cut(s) 1325
BstUI CGCG 2 cut(s) 1846, 2271
BstV2I GAAGAC 4 cut(s) 296, 1340, 2219, 2303
BstX2I RGATCY 2 cut(s) 1656, 1671
BstXI CCANNNNNNTGG 1 cut(s) 1975
BstYI RGATCY 2 cut(s) 1656, 1671
Bsu36I CCTNAGG 1 cut(s) 1245
BsuI GTATCC 1 cut(s) 2031
BsuRI GGCC 6 cut(s) 20, 228, 315, 869, 1724, 2312
BtgI CCRYGG 1 cut(s) 15
BtrI CACGTC 1 cut(s) 873
BtsIMutI CAGTG 1 cut(s) 130
BtuMI TCGCGA 1 cut(s) 2271
BveI ACCTGC 2 cut(s) 1070, 1614
Cac8I GCNNGC 5 cut(s) 1844, 1977, 1981, 2617, 2686
CaiI CAGNNNCTG 1 cut(s) 1532
CciI TCATGA 1 cut(s) 561
CfoI GCGC 1 cut(s) 175
Cfr10I RCCGGY 2 cut(s) 215, 2426
Cfr13I GGNCC 3 cut(s) 179, 1923, 2767
CseI GACGC 1 cut(s) 2677
Csp6I GTAC 6 cut(s) 685, 785, 1291, 1360, 1576, 2605
CspAI ACCGGT 1 cut(s) 215
CspCI CAANNNNNGTGG 2 cut(s) 1409, 1444
CviQI GTAC 6 cut(s) 685, 785, 1291, 1360, 1576, 2605
DdeI CTNAG 5 cut(s) 233, 1245, 1404, 1533, 1891
DraI TTTAAA 1 cut(s) 1644
EaeI YGGCCR 2 cut(s) 18, 867
Eam1104I CTCTTC 3 cut(s) 88, 847, 2009
EarI CTCTTC 3 cut(s) 88, 847, 2009
Ecl136II GAGCTC 1 cut(s) 1539
Eco130I CCWWGG 2 cut(s) 15, 2146
Eco147I AGGCCT 1 cut(s) 315
Eco24I GRGCYC 2 cut(s) 1541, 2572
Eco47I GGWCC 3 cut(s) 179, 1923, 2767
Eco53kI GAGCTC 1 cut(s) 1539
Eco57I CTGAAG 2 cut(s) 1686, 1932
Eco81I CCTNAGG 1 cut(s) 1245
EcoICRI GAGCTC 1 cut(s) 1539
EcoO109I RGGNCCY 1 cut(s) 2767
EcoRI GAATTC 3 cut(s) 373, 565, 1408
EcoRII CCWGG 4 cut(s) 310, 346, 1273, 1719
EcoT14I CCWWGG 2 cut(s) 15, 2146
EcoT22I ATGCAT 2 cut(s) 1636, 2554
EcoT38I GRGCYC 2 cut(s) 1541, 2572
ErhI CCWWGG 2 cut(s) 15, 2146
FalI AAGNNNNNCTT 2 cut(s) 2288, 2320
FaqI GGGAC 2 cut(s) 346, 2753
FauI CCCGC 1 cut(s) 1757
FbaI TGATCA 5 cut(s) 30, 127, 318, 612, 2389
FblI GTMKAC 1 cut(s) 957
FriOI GRGCYC 2 cut(s) 1541, 2572
FspBI CTAG 3 cut(s) 176, 294, 573
GlaI GCGC 1 cut(s) 174
HaeIII GGCC 6 cut(s) 20, 228, 315, 869, 1724, 2312
HapII CCGG 7 cut(s) 216, 468, 492, 669, 726, 2154, 2427
HgaI GACGC 1 cut(s) 2677
HhaI GCGC 1 cut(s) 175
Hin1I GRCGYC 1 cut(s) 2688
Hin6I GCGC 1 cut(s) 173
HinP1I GCGC 1 cut(s) 173
HindIII AAGCTT 2 cut(s) 832, 902
HpaII CCGG 7 cut(s) 216, 468, 492, 669, 726, 2154, 2427
HphI GGTGA 6 cut(s) 67, 362, 1118, 2203, 2576, 2765
Hpy166II GTNNAC 5 cut(s) 687, 958, 1293, 1360, 2115
Hpy188I TCNGA 9 cut(s) 693, 709, 877, 918, 1157, 1269, 1446, 1519, 1858
Hpy8I GTNNAC 5 cut(s) 687, 958, 1293, 1360, 2115
Hpy99I CGWCG 2 cut(s) 2276, 2693
HpyAV CCTTC 9 cut(s) 239, 440, 975, 1010, 1254, 1661, 1682, 2758, 2777
HpyCH4III ACNGT 4 cut(s) 134, 395, 2588, 2776
HpyCH4IV ACGT 4 cut(s) 872, 1232, 1356, 2139
HpyF10VI GCNNNNNNNGC 7 cut(s) 181, 1698, 1707, 1839, 2255, 2342, 2621
HpyF3I CTNAG 5 cut(s) 233, 1245, 1404, 1533, 1891
HpySE526I ACGT 4 cut(s) 872, 1232, 1356, 2139
Hsp92I GRCGYC 1 cut(s) 2688
HspAI GCGC 1 cut(s) 173
Ksp22I TGATCA 5 cut(s) 30, 127, 318, 612, 2389
LguI GCTCTTC 1 cut(s) 847
LmnI GCTCC 2 cut(s) 1188, 1534
LweI GCATC 7 cut(s) 651, 913, 1018, 1063, 1479, 1608, 1643
MaeI CTAG 3 cut(s) 176, 294, 573
MaeII ACGT 4 cut(s) 872, 1232, 1356, 2139
MaeIII GTNAC 8 cut(s) 206, 389, 456, 500, 577, 1201, 1214, 2776
MflI RGATCY 2 cut(s) 1656, 1671
MhlI GDGCHC 4 cut(s) 1325, 1541, 2469, 2572
MlsI TGGCCA 2 cut(s) 20, 869
MluNI TGGCCA 2 cut(s) 20, 869
MlyI GAGTC 3 cut(s) 1217, 2293, 2609
MmeI TCCRAC 3 cut(s) 896, 1826, 2500
Mox20I TGGCCA 2 cut(s) 20, 869
Mph1103I ATGCAT 2 cut(s) 1636, 2554
MroXI GAANNNNTTC 2 cut(s) 520, 721
MscI TGGCCA 2 cut(s) 20, 869
MseI TTAA 8 cut(s) 431, 438, 546, 1125, 1643, 1886, 2096, 2172
MslI CAYNNNNRTG 2 cut(s) 26, 2051
Msp20I TGGCCA 2 cut(s) 20, 869
MspA1I CMGCKG 1 cut(s) 1045
MspI CCGG 7 cut(s) 216, 468, 492, 669, 726, 2154, 2427
MspR9I CCNGG 6 cut(s) 312, 348, 469, 669, 1275, 1721
Mva1269I GAATGC 1 cut(s) 2724
MvaI CCWGG 4 cut(s) 312, 348, 1275, 1721
MvnI CGCG 2 cut(s) 1846, 2271
MwoI GCNNNNNNNGC 7 cut(s) 181, 1698, 1707, 1839, 2255, 2342, 2621
NciI CCSGG 2 cut(s) 469, 669
NcoI CCATGG 1 cut(s) 15
NlaIV GGNNCC 4 cut(s) 490, 2152, 2544, 2769
NmuCI GTSAC 2 cut(s) 577, 1201
NruI TCGCGA 1 cut(s) 2271
NsiI ATGCAT 2 cut(s) 1636, 2554
NspI RCATGY 3 cut(s) 1983, 2476, 2552
PaeI GCATGC 1 cut(s) 1983
PagI TCATGA 1 cut(s) 561
PaqCI CACCTGC 1 cut(s) 1614
PceI AGGCCT 1 cut(s) 315
PciI ACATGT 1 cut(s) 2472
PciSI GCTCTTC 1 cut(s) 847
PctI GAATGC 1 cut(s) 2724
PdmI GAANNNNTTC 2 cut(s) 520, 721
PfeI GAWTC 9 cut(s) 11, 325, 1065, 1270, 1366, 1459, 1514, 1520, 1751
PflFI GACNNNGTC 1 cut(s) 1207
PflMI CCANNNNNTGG 1 cut(s) 2646
PfoI TCCNGGA 1 cut(s) 467
PinAI ACCGGT 1 cut(s) 215
PleI GAGTC 3 cut(s) 1216, 2293, 2608
PpsI GAGTC 3 cut(s) 1216, 2293, 2608
PpuMI RGGWCCY 1 cut(s) 2767
PscI ACATGT 1 cut(s) 2472
PshBI ATTAAT 1 cut(s) 431
Psp124BI GAGCTC 1 cut(s) 1541
Psp5II RGGWCCY 1 cut(s) 2767
Psp6I CCWGG 4 cut(s) 310, 346, 1273, 1719
PspGI CCWGG 4 cut(s) 310, 346, 1273, 1719
PspN4I GGNNCC 4 cut(s) 490, 2152, 2544, 2769
PspPI GGNCC 3 cut(s) 179, 1923, 2767
PspPPI RGGWCCY 1 cut(s) 2767
PsrI GAACNNNNNNTAC 4 cut(s) 472, 504, 668, 700
PstI CTGCAG 5 cut(s) 91, 1144, 1715, 2235, 2626
PstNI CAGNNNCTG 1 cut(s) 1532
PsuI RGATCY 2 cut(s) 1656, 1671
PsyI GACNNNGTC 1 cut(s) 1207
PvuII CAGCTG 1 cut(s) 1045
RruI TCGCGA 1 cut(s) 2271
RsaI GTAC 6 cut(s) 686, 786, 1292, 1361, 1577, 2606
RsaNI GTAC 6 cut(s) 685, 785, 1291, 1360, 1576, 2605
RseI CAYNNNNRTG 2 cut(s) 26, 2051
SacI GAGCTC 1 cut(s) 1541
SapI GCTCTTC 1 cut(s) 847
SaqAI TTAA 8 cut(s) 431, 438, 546, 1125, 1643, 1886, 2096, 2172
Sau96I GGNCC 3 cut(s) 179, 1923, 2767
SchI GAGTC 3 cut(s) 1217, 2293, 2609
ScrFI CCNGG 6 cut(s) 312, 348, 469, 669, 1275, 1721
SduI GDGCHC 4 cut(s) 1325, 1541, 2469, 2572
SfaNI GCATC 7 cut(s) 651, 913, 1018, 1063, 1479, 1608, 1643
SfcI CTRYAG 7 cut(s) 87, 1140, 1524, 1711, 1755, 2231, 2622
SgrAI CRCCGGYG 1 cut(s) 215
SinI GGWCC 3 cut(s) 179, 1923, 2767
SmiMI CAYNNNNRTG 2 cut(s) 26, 2051
SmlI CTYRAG 3 cut(s) 790, 829, 2457
SmoI CTYRAG 3 cut(s) 790, 829, 2457
SpeI ACTAGT 1 cut(s) 572
SphI GCATGC 1 cut(s) 1983
SseBI AGGCCT 1 cut(s) 315
SsiI CCGC 6 cut(s) 182, 222, 594, 1132, 1764, 1921
SspI AATATT 1 cut(s) 1582
SspMI CTAG 3 cut(s) 176, 294, 573
SstI GAGCTC 1 cut(s) 1541
StuI AGGCCT 1 cut(s) 315
StyD4I CCNGG 6 cut(s) 310, 346, 467, 667, 1273, 1719
StyI CCWWGG 2 cut(s) 15, 2146
TaaI ACNGT 4 cut(s) 134, 395, 2588, 2776
TaiI ACGT 4 cut(s) 875, 1235, 1359, 2142
TaqI TCGA 7 cut(s) 328, 948, 979, 990, 1063, 1487, 2536
TatI WGTACW 3 cut(s) 684, 784, 1290
TfiI GAWTC 9 cut(s) 11, 325, 1065, 1270, 1366, 1459, 1514, 1520, 1751
Tru1I TTAA 8 cut(s) 431, 438, 546, 1125, 1643, 1886, 2096, 2172
Tru9I TTAA 8 cut(s) 431, 438, 546, 1125, 1643, 1886, 2096, 2172
TscAI CASTG 1 cut(s) 137
TseFI GTSAC 2 cut(s) 577, 1201
Tsp45I GTSAC 2 cut(s) 577, 1201
TspDTI ATGAA 9 cut(s) 129, 524, 550, 578, 689, 1695, 1810, 2041, 2373
TspGWI ACGGA 4 cut(s) 317, 1359, 1563, 2213
TspRI CASTG 1 cut(s) 137
Tth111I GACNNNGTC 1 cut(s) 1207
Van91I CCANNNNNTGG 1 cut(s) 2646
VpaK11BI GGWCC 3 cut(s) 179, 1923, 2767
VspI ATTAAT 1 cut(s) 431
XapI RAATTY 5 cut(s) 373, 565, 950, 1408, 2576
XceI RCATGY 3 cut(s) 1983, 2476, 2552
XcmI CCANNNNNNNNNTGG 3 cut(s) 13, 877, 1933
XmiI GTMKAC 1 cut(s) 957
XmnI GAANNNNTTC 2 cut(s) 520, 721
XspI CTAG 3 cut(s) 176, 294, 573
Zsp2I ATGCAT 2 cut(s) 1636, 2554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.