MD03G1227000.v1.1

Protein of unknown function (DUF2921)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000420
Physical Location & Seq
Reverse (-)
31239150 .. 31242353
3204 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1227000.v1.1.491

Sequence Viewer

Length: 1284 bp
ATGCAACTGATTCCATTATTAATAGTACTCGTGGGTGATTGTTCAACAAGATTGAGCTTGAGATTTCCTGCAATATTGACAATTCAAAACACGAGTAGCAATGTGGGGCAGATTTGGAGCAAGAAAACTGTGTCAGAGTCAGGTTACTTCGAAAAGATCACATTTGAAAGTCCACAAAATGAAAATAGAGGGTTTCTACTTCCAGGCCAGAAATACGAGAACACGAAAATTGACAGAGTAAAGAAGCTATGTCCGAGAAAGGAAACTGGCGTTAGTGCTATTAACAAGGCTAACAGATACCCAAGTCCTTTTTCTTATGACATGAGATTCGACATGTCAGCAAAAAGTTCTAAAGGAGGAATCTCTTGGGGCAGTTCTGTCCCTTTCTTTGTTGGGAACCGGTTTTATAACCAGCATTGGTATTCAATGAAGAATGAAAACTCAGTTGCGAGCATTGAAGGGTACTTCCATATTTCTGCTGAAGGGATCTATGGTCAAACAGAAGGAAGCCTATGCATGGTAGGATGTAGAGTTCTTGGCTCAGAGAGTCCGCAACCAACTGATTTGGTTGATTGTGATATTGTTGTCAATTTTCAGTTCCGACCGTCAAAATCTTTTGGTTATATCAAAGGAAGCATTATAAGCACGCGAATAAAGTATGATCCTCTTTATTTTGAACATTTGGATTTGACCTCAGCTGCCGGTTTTGTGAAAGAAGTAAGTAGATCAATCTGGAGGATGAATGTGGAGGTCACATTGGTTCTTTTGTCCGCCACACTTGCATGCGTATTTGTGGCTATGTTCACAAATAACACCAAGCACCGAAAAGGATTCCTCGGAAGTGGTGGATGGCTTGAAGTTAATGAGGTGATTGTGAGGGCCTTAACCATCGTAGGTTTCTTGTTGAAACTGCGTCTTTTGCAGTTAGCATGGACTGCAAGATCTGAACACAACGAAAGCCAAAATGAGCTTTGGGTCATGGGGAAGAAGTCTTTCGTTGTGGCTTCAATAGTATATGCTGCGGGGGCTTTGGCCGCATTGTTCCTAATGGATTGGAGGAAGATTGGCACTGGTAATGATGTTAAGTTAAACGAGTCATACATTTATGCAAGTCCCGCAGATGATTTTTACTCCGCTGCATGGGATGTCATCGTACCTTTTGGAGGTCTACTGTTTGCCGGCATCATTTACTTGCAGCACAAGTTTGGGGCCCGTTGCATTCTTCCTCAAAAGGTGAGAGAGTTGTGTGAATATGCGAAGGTCGCTGCAGTTAATGAAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
DUF2921

Protein Analysis

428

Amino Acids

47.72

Weight (kDa)

9.01

Isoelectric Point (pI)

34.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2921_N PF25333 11 - 54 3.4e-10 DUF2921 lipocalin-like domain
DUF2921_N PF25333 132 - 228 1.3e-20 DUF2921 lipocalin-like domain
DUF2921 PF11145 367 - 411 1.2e-06 Transmembrane E3 ligase/DUF2921 transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21700
fragaria_vesca FvH4_1g10360 FvH4_1g10760 FvH4_2g30370 FvH4_3g09240 FvH4_3g20050 FvH4_3g20280 FvH4_3g20290 FvH4_3g20291 FvH4_3g20340 FvH4_3g20350 FvH4_3g20351 FvH4_3g20380 FvH4_3g20390 FvH4_5g13190 FvH4_6g20070 FvH4_6g42890
malus_domestica MD03G1227000.v1.1 MD10G1294900.v1.1 MD11G1246300.v1.1 MD11G1246400.v1.1 MD11G1246500.v1.1 MD11G1246600.v1.1
prunus_persica Prupe.4G047300_v2.0.a1 Prupe.4G181700_v2.0.a1 Prupe.4G181900_v2.0.a1 Prupe.4G182000_v2.0.a1 Prupe.4G182200_v2.0.a1
pyrus_communis pycom03g17470 pycom10g24670 pycom11g21640
rosa_chinensis RchiOBHm_Chr5g0008381 RchiOBHm_Chr5g0008391 RchiOBHm_Chr5g0033881 RchiOBHm_Chr5g0034171 RchiOBHm_Chr5g0034181 RchiOBHm_Chr5g0034191 RchiOBHm_Chr5g0034201 RchiOBHm_Chr5g0034211 RchiOBHm_Chr5g0034221 RchiOBHm_Chr5g0034231 RchiOBHm_Chr5g0034271 RchiOBHm_Chr5g0034281
rosa_laevigata RLG00000031566 RLG00000031567 RLG00000033506 RLG00000033529 RLG00000033530 RLG00000033531
rosa_multiflora Rmu_co8445509.1_g000001 Rmu_co8470615.1_g000001 Rmu_sc0008805.1_g000003 Rmu_sc0008805.1_g000004 Rmu_ssc0000128.1_g000029 Rmu_ssc0000128.1_g000031 Rmu_ssc0000128.1_g000032 Rmu_ssc0000388.1_g000062 Rmu_ssc0000388.1_g000063
rosa_roxburghii Rroxscaffold_1G00046010 Rroxscaffold_1G00046020 Rroxscaffold_1G00046030 Rroxscaffold_1G00046040 Rroxscaffold_1G00046060 Rroxscaffold_1G00046070 Rroxscaffold_1G00046080 Rroxscaffold_1G00046090
rosa_rugosa Rorug04G0435500 Rorug05G0142900 Rorug05G0144600 Rorug05G0144600 Rorug05G0144700 Rorug05G0144800
rosa_samantha Rh5AG233800 Rh5AG235900 Rh5AG236000 Rh5AG236100 Rh5AG236200 Rh5AG236300 Rh5AG236500 Rh5BG061200 Rh5BG061300 Rh5BG234300 Rh5BG236600 Rh5BG236800 Rh5CG071200 Rh5DG060000 Rh5DG060100 Rh5DG241300 Rh5DG243700 Rh5DG244000 Rh5DG244100
rosa_wichuraiana Rw3G018950 Rw5G005680 Rw5G021330 Rw5G021340 Rw5G021350 Rw5G021550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 408, 641
AccI GTMKAC 1 cut(s) 1168
AccII CGCG 1 cut(s) 649
AciI CCGC 6 cut(s) 551, 771, 1022, 1035, 1116, 1134
AclWI GGATC 2 cut(s) 494, 656
AcoI YGGCCR 1 cut(s) 1032
AcuI CTGAAG 1 cut(s) 501
AfaI GTAC 3 cut(s) 27, 464, 1155
AfiI CCNNNNNNNGG 3 cut(s) 517, 1140, 1163
AflIII ACRYGT 1 cut(s) 333
AgeI ACCGGT 1 cut(s) 399
AgsI TTSAA 9 cut(s) 45, 86, 167, 426, 458, 677, 857, 907, 1008
AjnI CCWGG 1 cut(s) 202
AjuI GAANNNNNNNTTGG 2 cut(s) 295, 327
AloI GAACNNNNNNTCC 2 cut(s) 516, 548
AluBI AGCT 4 cut(s) 57, 247, 698, 970
AluI AGCT 4 cut(s) 57, 247, 698, 970
AlwI GGATC 2 cut(s) 494, 656
AoxI GGCC 4 cut(s) 205, 879, 1032, 1209
ApaI GGGCCC 1 cut(s) 1213
ApeKI GCWGC 5 cut(s) 698, 1019, 1136, 1195, 1265
ArsI GACNNNNNNTTYG 2 cut(s) 311, 343
AseI ATTAAT 1 cut(s) 20
AsiGI ACCGGT 1 cut(s) 399
Asp700I GAANNNNTTC 1 cut(s) 992
AspS9I GGNCC 3 cut(s) 879, 1209, 1210
AsuHPI GGTGA 3 cut(s) 47, 880, 1246
AsuII TTCGAA 1 cut(s) 150
BaeGI GKGCMC 1 cut(s) 1213
BaeI ACNNNNGTAYC 2 cut(s) 289, 322
BanII GRGCYC 1 cut(s) 1213
BauI CACGAG 2 cut(s) 29, 91
BbvCI CCTCAGC 1 cut(s) 694
BbvI GCAGC 5 cut(s) 685, 1006, 1123, 1207, 1252
BccI CCATC 2 cut(s) 843, 896
BciT130I CCWGG 1 cut(s) 204
BfmI CTRYAG 1 cut(s) 1266
BglII AGATCT 1 cut(s) 941
BisI GCNGC 6 cut(s) 699, 1020, 1035, 1137, 1196, 1266
BlsI GCNGC 6 cut(s) 700, 1021, 1036, 1138, 1197, 1267
BmcAI AGTACT 1 cut(s) 27
Bme1390I CCNGG 1 cut(s) 204
BmgT120I GGNCC 3 cut(s) 879, 1209, 1210
BmiI GGNNCC 3 cut(s) 398, 1210, 1211
BmrFI CCNGG 1 cut(s) 204
BmsI GCATC 1 cut(s) 1191
BpmI CTGGAG 1 cut(s) 754
Bpu10I CCTNAGC 1 cut(s) 694
Bpu14I TTCGAA 1 cut(s) 150
BpuEI CTTGAG 1 cut(s) 79
BsaJI CCNNGG 1 cut(s) 835
BsaWI WCCGGW 1 cut(s) 399
Bsc4I CCNNNNNNNGG 3 cut(s) 517, 1140, 1163
Bse118I RCCGGY 3 cut(s) 399, 701, 1178
Bse1I ACTGG 2 cut(s) 271, 1075
Bse3DI GCAATG 1 cut(s) 106
BseBI CCWGG 1 cut(s) 204
BseDI CCNNGG 1 cut(s) 835
BseGI GGATG 4 cut(s) 530, 744, 854, 1150
BseLI CCNNNNNNNGG 3 cut(s) 517, 1140, 1163
BseMI GCAATG 1 cut(s) 106
BseMII CTCAG 3 cut(s) 456, 555, 708
BseNI ACTGG 2 cut(s) 271, 1075
BseSI GKGCMC 1 cut(s) 1213
BseXI GCAGC 5 cut(s) 685, 1006, 1123, 1207, 1252
Bsh1236I CGCG 1 cut(s) 649
Bsh1285I CGRYCG 1 cut(s) 605
BshFI GGCC 4 cut(s) 207, 881, 1034, 1211
BshTI ACCGGT 1 cut(s) 399
BsiEI CGRYCG 1 cut(s) 605
BsiSI CCGG 3 cut(s) 400, 702, 1179
BslFI GGGAC 2 cut(s) 365, 1098
BslI CCNNNNNNNGG 3 cut(s) 517, 1140, 1163
BsmFI GGGAC 2 cut(s) 365, 1098
BsmI GAATGC 1 cut(s) 1218
BsnI GGCC 4 cut(s) 207, 881, 1034, 1211
Bsp119I TTCGAA 1 cut(s) 150
Bsp120I GGGCCC 1 cut(s) 1209
Bsp1286I GDGCHC 1 cut(s) 1213
Bsp143I GATC 5 cut(s) 156, 486, 661, 725, 941
BspACI CCGC 6 cut(s) 551, 771, 1022, 1035, 1116, 1134
BspANI GGCC 4 cut(s) 207, 881, 1034, 1211
BspCNI CTCAG 3 cut(s) 455, 554, 707
BspFNI CGCG 1 cut(s) 649
BspLI GGNNCC 3 cut(s) 398, 1210, 1211
BspMAI CTGCAG 1 cut(s) 1270
BspPI GGATC 2 cut(s) 494, 656
BspT104I TTCGAA 1 cut(s) 150
BsrDI GCAATG 1 cut(s) 106
BsrFI RCCGGY 3 cut(s) 399, 701, 1178
BsrI ACTGG 2 cut(s) 271, 1075
BssAI RCCGGY 3 cut(s) 399, 701, 1178
BssECI CCNNGG 1 cut(s) 835
BssMI GATC 5 cut(s) 156, 486, 661, 725, 941
BssSI CACGAG 2 cut(s) 29, 91
Bst2BI CACGAG 2 cut(s) 29, 91
Bst2UI CCWGG 1 cut(s) 204
Bst4CI ACNGT 3 cut(s) 130, 606, 1173
BstAPI GCANNNNNTGC 1 cut(s) 935
BstBI TTCGAA 1 cut(s) 150
BstC8I GCNNGC 4 cut(s) 451, 647, 784, 1180
BstDEI CTNAG 3 cut(s) 442, 541, 694
BstENI CCTNNNNNAGG 1 cut(s) 1161
BstF5I GGATG 4 cut(s) 530, 744, 854, 1150
BstFNI CGCG 1 cut(s) 649
BstKTI GATC 5 cut(s) 159, 489, 664, 728, 944
BstMBI GATC 5 cut(s) 156, 486, 661, 725, 941
BstMCI CGRYCG 1 cut(s) 605
BstMWI GCNNNNNNNGC 8 cut(s) 642, 779, 919, 935, 1025, 1034, 1115, 1262
BstNI CCWGG 1 cut(s) 204
BstNSI RCATGY 2 cut(s) 337, 786
BstSCI CCNGG 1 cut(s) 202
BstSFI CTRYAG 1 cut(s) 1266
BstSLI GKGCMC 1 cut(s) 1213
BstUI CGCG 1 cut(s) 649
BstV1I GCAGC 5 cut(s) 685, 1006, 1123, 1207, 1252
BstX2I RGATCY 2 cut(s) 486, 941
BstYI RGATCY 2 cut(s) 486, 941
BsuRI GGCC 4 cut(s) 207, 881, 1034, 1211
BtsCI GGATG 4 cut(s) 530, 744, 854, 1150
BtsIMutI CAGTG 1 cut(s) 1068
Cac8I GCNNGC 4 cut(s) 451, 647, 784, 1180
Cfr10I RCCGGY 3 cut(s) 399, 701, 1178
Cfr13I GGNCC 3 cut(s) 879, 1209, 1210
CseI GACGC 1 cut(s) 902
Csp6I GTAC 3 cut(s) 26, 463, 1154
CspAI ACCGGT 1 cut(s) 399
CviAII CATG 7 cut(s) 322, 334, 517, 783, 930, 979, 1140
CviQI GTAC 3 cut(s) 26, 463, 1154
DdeI CTNAG 3 cut(s) 442, 541, 694
DpnI GATC 5 cut(s) 158, 488, 663, 727, 943
DpnII GATC 5 cut(s) 156, 486, 661, 725, 941
EaeI YGGCCR 1 cut(s) 1032
EciI GGCGGA 1 cut(s) 760
Eco24I GRGCYC 1 cut(s) 1213
Eco57I CTGAAG 1 cut(s) 501
EcoNI CCTNNNNNAGG 1 cut(s) 1161
EcoO109I RGGNCCY 2 cut(s) 879, 1209
EcoRII CCWGG 1 cut(s) 202
EcoT22I ATGCAT 1 cut(s) 518
EcoT38I GRGCYC 1 cut(s) 1213
FaeI CATG 7 cut(s) 325, 337, 520, 786, 933, 982, 1143
FaqI GGGAC 2 cut(s) 365, 1098
FatI CATG 7 cut(s) 321, 333, 516, 782, 929, 978, 1139
FauI CCCGC 2 cut(s) 1015, 1123
FblI GTMKAC 1 cut(s) 1168
Fnu4HI GCNGC 6 cut(s) 699, 1020, 1035, 1137, 1196, 1266
FokI GGATG 4 cut(s) 537, 751, 861, 1157
FriOI GRGCYC 1 cut(s) 1213
Fsp4HI GCNGC 6 cut(s) 699, 1020, 1035, 1137, 1196, 1266
GluI GCNGC 6 cut(s) 699, 1020, 1035, 1137, 1196, 1266
GsuI CTGGAG 1 cut(s) 754
HaeIII GGCC 4 cut(s) 207, 881, 1034, 1211
HapII CCGG 3 cut(s) 400, 702, 1179
HgaI GACGC 1 cut(s) 902
Hin1II CATG 7 cut(s) 325, 337, 520, 786, 933, 982, 1143
HinfI GANTC 7 cut(s) 10, 137, 327, 360, 547, 831, 1094
HpaII CCGG 3 cut(s) 400, 702, 1179
HphI GGTGA 3 cut(s) 47, 880, 1246
Hpy166II GTNNAC 3 cut(s) 173, 804, 1169
Hpy188I TCNGA 6 cut(s) 136, 255, 544, 602, 839, 946
Hpy188III TCNNGA 1 cut(s) 733
Hpy8I GTNNAC 3 cut(s) 173, 804, 1169
HpyAV CCTTC 5 cut(s) 452, 476, 497, 1252, 1271
HpyCH4III ACNGT 3 cut(s) 130, 606, 1173
HpyF10VI GCNNNNNNNGC 8 cut(s) 642, 779, 919, 935, 1025, 1034, 1115, 1262
HpyF3I CTNAG 3 cut(s) 442, 541, 694
Hsp92II CATG 7 cut(s) 325, 337, 520, 786, 933, 982, 1143
KroI GCCGGC 1 cut(s) 1178
KroNI GCCGGC 1 cut(s) 1180
Kzo9I GATC 5 cut(s) 156, 486, 661, 725, 941
LmnI GCTCC 1 cut(s) 117
Lsp1109I GCAGC 5 cut(s) 685, 1006, 1123, 1207, 1252
LweI GCATC 1 cut(s) 1191
MaeIII GTNAC 2 cut(s) 143, 751
MalI GATC 5 cut(s) 158, 488, 663, 727, 943
MboI GATC 5 cut(s) 156, 486, 661, 725, 941
MboII GAAGA 4 cut(s) 442, 997, 1072, 1214
MflI RGATCY 2 cut(s) 486, 941
MhlI GDGCHC 1 cut(s) 1213
MluCI AATT 3 cut(s) 81, 228, 589
MlyI GAGTC 3 cut(s) 146, 556, 1103
MmeI TCCRAC 1 cut(s) 625
Mph1103I ATGCAT 1 cut(s) 518
MroNI GCCGGC 1 cut(s) 1178
MroXI GAANNNNTTC 1 cut(s) 992
MseI TTAA 7 cut(s) 20, 282, 861, 884, 1083, 1088, 1272
MslI CAYNNNNRTG 1 cut(s) 781
MspA1I CMGCKG 2 cut(s) 698, 1136
MspI CCGG 3 cut(s) 400, 702, 1179
MspR9I CCNGG 1 cut(s) 204
Mva1269I GAATGC 1 cut(s) 1218
MvaI CCWGG 1 cut(s) 204
MvnI CGCG 1 cut(s) 649
MwoI GCNNNNNNNGC 8 cut(s) 642, 779, 919, 935, 1025, 1034, 1115, 1262
NaeI GCCGGC 1 cut(s) 1180
NdeII GATC 5 cut(s) 156, 486, 661, 725, 941
NgoMIV GCCGGC 1 cut(s) 1178
NlaIII CATG 7 cut(s) 325, 337, 520, 786, 933, 982, 1143
NlaIV GGNNCC 3 cut(s) 398, 1210, 1211
NmuCI GTSAC 1 cut(s) 751
NsiI ATGCAT 1 cut(s) 518
NspI RCATGY 2 cut(s) 337, 786
NspV TTCGAA 1 cut(s) 150
PaeI GCATGC 1 cut(s) 786
PciI ACATGT 1 cut(s) 333
PctI GAATGC 1 cut(s) 1218
PdiI GCCGGC 1 cut(s) 1180
PdmI GAANNNNTTC 1 cut(s) 992
PfeI GAWTC 4 cut(s) 10, 327, 360, 831
PinAI ACCGGT 1 cut(s) 399
PkrI GCNGC 6 cut(s) 700, 1021, 1036, 1138, 1197, 1267
PleI GAGTC 3 cut(s) 145, 555, 1102
PpsI GAGTC 3 cut(s) 145, 555, 1102
PscI ACATGT 1 cut(s) 333
PshBI ATTAAT 1 cut(s) 20
PsiI TTATAA 2 cut(s) 408, 641
Psp6I CCWGG 1 cut(s) 202
PspGI CCWGG 1 cut(s) 202
PspN4I GGNNCC 3 cut(s) 398, 1210, 1211
PspOMI GGGCCC 1 cut(s) 1209
PspPI GGNCC 3 cut(s) 879, 1209, 1210
PstI CTGCAG 1 cut(s) 1270
PsuI RGATCY 2 cut(s) 486, 941
PvuII CAGCTG 1 cut(s) 698
RsaI GTAC 3 cut(s) 27, 464, 1155
RsaNI GTAC 3 cut(s) 26, 463, 1154
RseI CAYNNNNRTG 1 cut(s) 781
SaqAI TTAA 7 cut(s) 20, 282, 861, 884, 1083, 1088, 1272
SatI GCNGC 6 cut(s) 699, 1020, 1035, 1137, 1196, 1266
Sau3AI GATC 5 cut(s) 156, 486, 661, 725, 941
Sau96I GGNCC 3 cut(s) 879, 1209, 1210
ScaI AGTACT 1 cut(s) 27
SchI GAGTC 3 cut(s) 146, 556, 1103
ScrFI CCNGG 1 cut(s) 204
SduI GDGCHC 1 cut(s) 1213
SfaNI GCATC 1 cut(s) 1191
SfcI CTRYAG 1 cut(s) 1266
SfuI TTCGAA 1 cut(s) 150
SmiMI CAYNNNNRTG 1 cut(s) 781
SmlI CTYRAG 1 cut(s) 58
SmoI CTYRAG 1 cut(s) 58
SphI GCATGC 1 cut(s) 786
Sse9I AATT 3 cut(s) 81, 228, 589
SsiI CCGC 6 cut(s) 551, 771, 1022, 1035, 1116, 1134
SspI AATATT 1 cut(s) 75
StyD4I CCNGG 1 cut(s) 202
TaaI ACNGT 3 cut(s) 130, 606, 1173
TaqI TCGA 2 cut(s) 150, 330
TasI AATT 3 cut(s) 81, 228, 589
TatI WGTACW 1 cut(s) 25
TauI GCSGC 1 cut(s) 1037
TfiI GAWTC 4 cut(s) 10, 327, 360, 831
Tru1I TTAA 7 cut(s) 20, 282, 861, 884, 1083, 1088, 1272
Tru9I TTAA 7 cut(s) 20, 282, 861, 884, 1083, 1088, 1272
TscAI CASTG 1 cut(s) 1075
TseFI GTSAC 1 cut(s) 751
TseI GCWGC 5 cut(s) 698, 1019, 1136, 1195, 1265
Tsp45I GTSAC 1 cut(s) 751
TspDTI ATGAA 4 cut(s) 195, 443, 450, 755
TspRI CASTG 1 cut(s) 1075
VspI ATTAAT 1 cut(s) 20
XagI CCTNNNNNAGG 1 cut(s) 1161
XceI RCATGY 2 cut(s) 337, 786
XmiI GTMKAC 1 cut(s) 1168
XmnI GAANNNNTTC 1 cut(s) 992
ZrmI AGTACT 1 cut(s) 27
Zsp2I ATGCAT 1 cut(s) 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.