Rh5AG236500

Protein of unknown function (DUF2921)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000420
Physical Location & Seq
Reverse (-)
28690366 .. 28695565
5200 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG236500.1

Sequence Viewer

Length: 3210 bp
ATGGCCATTTTCATGAAAATTTTCATTTTCTTCTTTGTTTTTGTCACACGTTCGTTAAACTCTGTTTCGTCCTCCACTTCAGATGCTCAAGTGTATTTTGCTGATCATTGTGCTTCAATTGCTCCTCCTCACTACTCAACCCCACAAAGATATGCCGGACTTAATTCCTCTGTTCACCGCCTTACCGGTTTCTACTACAGTGCTGGTGAAACTAACATTCTTAGTCAGAACTCAATTGTATTCCGCTTCCGGAATGATGGAGCAGCGGATGAACAAGGCTTGTTCATGGTCCAAGGAAGTCTTCGATTTCCTAGTGACAGTACATATAACCTTGTGGGAAACTCTACAAGGAGCTGGCAATCCACCGACTTAAGAGTTCCATATGGACAGCGTCCCAGAAGCTTTAGGCTAAATGGGGTCTGGTCAGAATCTTCTGGGGAGCTTTGTATGGTTGGATCAGGTTCTAATTCCTCGGACCAAGGTAATTTACTGACCTTTCCTGTGGTTCTTAAACTACATAAACTCAAGAGTTCAAGTAGTCTTACTAGTTTAATTAGTGGCACCTTAGAGAGCTTGGTAACTTCTAAGAATGAACCAAATTATTTTGGACCAATCTCTATATCATTGCTTCCTTCCATGAACTACCAGTACACTTTTGTTTCAAACAAATCTCATAACAGCTATTCGGATGGAAGTGTTGTTCCCCCAAGTTCTTTAAAAATAGGGAGATTTTGCAGGGTATTCTCAAGAGCAATACTATTCCGTGAGTTACAACTGAGATATTCAAGCCATTGTGGTTCTGCAAACAACTGCTCTCCTCTTGCTGTGTCTGATTTGCCTCGCATCATGTCGTTGAATAATATTGAGTGTTTGGAGGATAGAAAAACATTGCGAGTTCTGGTAGATTTTGCAGCCAGTAGCATCAGTTACCAGAGGCCTTTCAATCCAACAACAACATTGGTTGCGGAAGGTTCATGGGATGCAAGGAAAAATCAGCTAAATGTTGTTGCTTGTCGGTTCGTGCATGCAAGGAATTCTTTTAGTCCTAGTCATGTGGGTGATTGTTCAACAAGATTGACAATGGGATTTCCTTCAATATGGACAATTGGAAATTCTAGCTGCATTGTGGGGAAAATTTGGAGCAACAAATCCGTGACAGAGGCAGGTTACTTCGAAAACATCACATTTGACAGTGCTCAAAGTGATTCAGCTGTATTTCCAGGCCAGAGGTATGAGTATACCAAAATAGACAAAGCAACAAAGTTGTGCCCAAGAAAGAAGACTGATGCTGATGACAAGAACAACATTTATCCAAATCCTTTTTCTTATTACATGGGATTGGATATTACAGCCAACAGTTCTACAGGAGAAGTTGGATGGGGCTATTCTGTCCCTCTCTCTGTTGATAACAAATTCTTCCAACCGTATTGGTACTCTCTGGAAGATGGAACTGAAGAATACCTTGGAGCTCCTGTGAGTGCTCCTGTGAGCGATAGAAACAACAATAGCAACAGGTACAACATCAGCTACAGAATAAGCATTAAAGCAGTAGATGGGGTTAGGCTTCCCAGTGGAGTGTCCCAACTTAATTCATTATATGAGATGGGGATTTTTGCTGAAGGGATATATGATGGCAGAGATGGAAGCCTGTGTATGGTAGGTTGCCGATACTTGAGCTCAAACAGTCACCAACAGCCAACATATGATTCTGTAGACTGTGAGATTGTTGTCAATGTTCAGTTTCCTCCAACAAATCCAAACAGTACCTATTCGGGTTTTATCAAGGGAAGCATCGAAAGCACACGAGAAATGTCTGATCCTCTTCATTTTGAGGGATTGGATTTGACCTCAGCTGCTGGTAACATGGTCGAAGCAAGCCAATCCATTTGGAGAATGGATGTGGAGATCATATTGGTTGTTATATCCAACACACTCGCTTGTGTTTTTGGGGCATTACAACTCTTTCATGTGAAACGGAATCCGGATGTGCTCCCATCCATTTCTATCTTGATGCTGTATATTCTTGCTCTTGGCTACATGATACCTCTTATGCTAAACTTTGATGCCTTGTTCACACAACATGGTAGCCAAACTGTGTTCCTTGGAAGTGGATGGCTTGAACTCAATGAAGTAATTGTAAGGGTAGTAACAATGGTAGGTTTCTTGCTGCAAATTCGTCTTCTGCAGATAACAATGTCAGCAAAATCAGCAAATGGAAACCAAAAGGAGCTCTGGGCAGTGGAGAAGCTGGCTCTCTTTGTGGCTTTACCAGTATATGTTGGAGGGTCCTTGGTTGCTTTGCTTCTGATGAACTGGAGGAGGAACGATATTGCTGTGATGGTTTCGAGTGGTCAAAAGCATCCCATTTTGGCTGTTCAAATTTCTTATGCTGACCATTGTGCTTCAGTTGTTCCTGAGTCAACCGAAAAAATAAATGCACGATCTCACTTGGTACATCTGATTCACACAGGTTACTACTACACTGGTGGTGGCAGTGACATTCTTAATCTATACTCGCCAAATGATCAGGTCAAAAATTCTATTGGCTTCCAATGCTGGGACATTAGAGAAACTAATGTACAAGGGTTGTTCAAGGTTGAAGGAACTCTTGAGTTTCAAGTAACCCACAGGCATGAGCAGCAAAGCCTGCCAGTTCAAACATTTCCAGGTTCCGTTTCCTTTAGACTAAATGGATTCTGGTCAGAATCTTCTGGGAAGCTTTGCATGGTCGGATCATCATCCATTTACTCAGCACAAGGTAAACTTCTTGTTCTTAAGCTGTACAATATCATGAATTCCACTAGTGTTACAAGTTTGATTAGTGGAACCTTCGAGCATGTCATGATAACTGAGAATGATCCCAAGTATTTGGGACCAATCTCTATTTTGCTATTCCCTCGCATGAACTACAAGTACACTTTGGTCTCAACTAATTCTAATAACAGTTGCTCTGGTGGAAGTGAAGATACCCCAAGCTCTTTACAAGTAGAGAGATTTTGTTCACTACTATCATCAGTGGTACTAAATCATGACTTTCAACTCAAATATTCAAGCCACTGTGTTACTGCAAAGAACTGCACCCCAATTTCTATGTCTGATTGGCCTTGGGCTGTATCGTTGAAGGACATTGTGTGTTCAGAGGATAAACGAAGGGGACAATTGGAAGTACCAGCAGCATTGTGGGGCATATTTGGAGCACCAAAACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
DUF2921

Protein Analysis

1069

Amino Acids

118.36

Weight (kDa)

7.05

Isoelectric Point (pI)

41.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2921_N PF25333 62 - 206 6.4e-18 DUF2921 lipocalin-like domain
DUF2921_N PF25333 227 - 394 1.8e-25 DUF2921 lipocalin-like domain
DUF2921_N PF25333 421 - 613 1.6e-31 DUF2921 lipocalin-like domain
DUF2921 PF11145 625 - 777 2.2e-29 Transmembrane E3 ligase/DUF2921 transmembrane domain
DUF2921_N PF25333 796 - 961 3.6e-18 DUF2921 lipocalin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21700
fragaria_vesca FvH4_1g10360 FvH4_1g10760 FvH4_2g30370 FvH4_3g09240 FvH4_3g20050 FvH4_3g20280 FvH4_3g20290 FvH4_3g20291 FvH4_3g20340 FvH4_3g20350 FvH4_3g20351 FvH4_3g20380 FvH4_3g20390 FvH4_5g13190 FvH4_6g20070 FvH4_6g42890
malus_domestica MD03G1227000.v1.1 MD10G1294900.v1.1 MD11G1246300.v1.1 MD11G1246400.v1.1 MD11G1246500.v1.1 MD11G1246600.v1.1
prunus_persica Prupe.4G047300_v2.0.a1 Prupe.4G181700_v2.0.a1 Prupe.4G181900_v2.0.a1 Prupe.4G182000_v2.0.a1 Prupe.4G182200_v2.0.a1
pyrus_communis pycom03g17470 pycom10g24670 pycom11g21640
rosa_chinensis RchiOBHm_Chr5g0008381 RchiOBHm_Chr5g0008391 RchiOBHm_Chr5g0033881 RchiOBHm_Chr5g0034171 RchiOBHm_Chr5g0034181 RchiOBHm_Chr5g0034191 RchiOBHm_Chr5g0034201 RchiOBHm_Chr5g0034211 RchiOBHm_Chr5g0034221 RchiOBHm_Chr5g0034231 RchiOBHm_Chr5g0034271 RchiOBHm_Chr5g0034281
rosa_laevigata RLG00000031566 RLG00000031567 RLG00000033506 RLG00000033529 RLG00000033530 RLG00000033531
rosa_multiflora Rmu_co8445509.1_g000001 Rmu_co8470615.1_g000001 Rmu_sc0008805.1_g000003 Rmu_sc0008805.1_g000004 Rmu_ssc0000128.1_g000029 Rmu_ssc0000128.1_g000031 Rmu_ssc0000128.1_g000032 Rmu_ssc0000388.1_g000062 Rmu_ssc0000388.1_g000063
rosa_roxburghii Rroxscaffold_1G00046010 Rroxscaffold_1G00046020 Rroxscaffold_1G00046030 Rroxscaffold_1G00046040 Rroxscaffold_1G00046060 Rroxscaffold_1G00046070 Rroxscaffold_1G00046080 Rroxscaffold_1G00046090
rosa_rugosa Rorug04G0435500 Rorug05G0142900 Rorug05G0144600 Rorug05G0144600 Rorug05G0144700 Rorug05G0144800
rosa_samantha Rh5AG233800 Rh5AG235900 Rh5AG236000 Rh5AG236100 Rh5AG236200 Rh5AG236300 Rh5AG236500 Rh5BG061200 Rh5BG061300 Rh5BG234300 Rh5BG236600 Rh5BG236800 Rh5CG071200 Rh5DG060000 Rh5DG060100 Rh5DG241300 Rh5DG243700 Rh5DG244000 Rh5DG244100
rosa_wichuraiana Rw3G018950 Rw5G005680 Rw5G021330 Rw5G021340 Rw5G021350 Rw5G021550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1154
AccB1I GGYRCC 1 cut(s) 560
AccI GTMKAC 2 cut(s) 1238, 1713
AccIII TCCGGA 2 cut(s) 249, 1981
AciI CCGC 4 cut(s) 178, 244, 266, 965
AclWI GGATC 4 cut(s) 463, 1811, 2740, 2852
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 9 cut(s) 18, 1033, 1111, 1134, 1412, 2172, 2379, 2536, 2794
AcuI CTGAAG 4 cut(s) 63, 1473, 1638, 2388
AfiI CCNNNNNNNGG 2 cut(s) 1654, 2557
AflII CTTAAG 2 cut(s) 370, 2774
AflIII ACRYGT 1 cut(s) 47
AgeI ACCGGT 1 cut(s) 185
AhlI ACTAGT 2 cut(s) 545, 2801
AjnI CCWGG 2 cut(s) 1219, 2665
AjuI GAANNNNNNNTTGG 8 cut(s) 285, 317, 1306, 1338, 1446, 1478, 1690, 1722
AloI GAACNNNNNNTCC 2 cut(s) 684, 716
Alw21I GWGCWC 7 cut(s) 1198, 1471, 1483, 1679, 1992, 2232, 3199
Alw26I GTCTC 1 cut(s) 2929
AlwI GGATC 4 cut(s) 463, 1811, 2740, 2852
AlwNI CAGNNNCTG 1 cut(s) 1856
Aor13HI TCCGGA 2 cut(s) 249, 1981
AoxI GGCC 4 cut(s) 3, 935, 1222, 3101
ApeKI GCWGC 7 cut(s) 263, 911, 1119, 1853, 2167, 2638, 3173
ApoI RAATTY 9 cut(s) 18, 1033, 1111, 1134, 1412, 2172, 2379, 2536, 2794
AsiGI ACCGGT 1 cut(s) 185
Asp700I GAANNNNTTC 1 cut(s) 20
AspS9I GGNCC 5 cut(s) 289, 475, 608, 2286, 2873
AsuHPI GGTGA 4 cut(s) 167, 218, 1070, 1679
AsuII TTCGAA 1 cut(s) 1173
AvaII GGWCC 5 cut(s) 289, 475, 608, 2286, 2873
BaeGI GKGCMC 1 cut(s) 1271
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 560
BanII GRGCYC 3 cut(s) 1471, 1679, 2232
BarI GAAGNNNNNNTAC 2 cut(s) 2950, 2982
BauI CACGAG 1 cut(s) 1803
BbsI GAAGAC 3 cut(s) 293, 1286, 2171
Bbv12I GWGCWC 7 cut(s) 1198, 1471, 1483, 1679, 1992, 2232, 3199
BbvCI CCTCAGC 1 cut(s) 1849
BbvI GCAGC 7 cut(s) 275, 923, 1106, 1840, 2154, 2650, 3185
BciT130I CCWGG 2 cut(s) 1221, 2667
BclI TGATCA 2 cut(s) 103, 2524
BcoDI GTCTC 1 cut(s) 2929
BcuI ACTAGT 2 cut(s) 545, 2801
BfaI CTAG 5 cut(s) 312, 546, 1047, 1116, 2802
BfmI CTRYAG 5 cut(s) 196, 1362, 1528, 1710, 2183
BfrI CTTAAG 2 cut(s) 370, 2774
BfuAI ACCTGC 1 cut(s) 1154
BisI GCNGC 7 cut(s) 264, 912, 1120, 1854, 2168, 2639, 3174
BlsI GCNGC 7 cut(s) 265, 913, 1121, 1855, 2169, 2640, 3175
Bme1390I CCNGG 2 cut(s) 1221, 2667
Bme18I GGWCC 5 cut(s) 289, 475, 608, 2286, 2873
BmgT120I GGNCC 5 cut(s) 289, 475, 608, 2286, 2873
BmiI GGNNCC 5 cut(s) 562, 2287, 2671, 2827, 2874
BmrFI CCNGG 2 cut(s) 1221, 2667
BmrI ACTGGG 1 cut(s) 1563
BmsI GCATC 9 cut(s) 73, 852, 930, 970, 1276, 1800, 2001, 2053, 2368
BmuI ACTGGG 1 cut(s) 1563
BpiI GAAGAC 3 cut(s) 293, 1286, 2171
BpmI CTGGAG 1 cut(s) 2335
Bpu10I CCTNAGC 1 cut(s) 1849
Bpu14I TTCGAA 1 cut(s) 1173
BpuEI CTTGAG 5 cut(s) 72, 509, 730, 1693, 2630
BsaBI GATNNNNATC 1 cut(s) 2737
BsaI GGTCTC 1 cut(s) 2929
BsaJI CCNNGG 7 cut(s) 292, 471, 478, 1462, 2101, 2289, 3104
BsaWI WCCGGW 3 cut(s) 185, 249, 1981
BsaXI ACNNNNNCTCC 2 cut(s) 1458, 1488
Bsc4I CCNNNNNNNGG 2 cut(s) 1654, 2557
Bse118I RCCGGY 1 cut(s) 185
Bse1I ACTGG 7 cut(s) 646, 915, 1569, 2270, 2318, 2488, 2651
Bse3DI GCAATG 2 cut(s) 623, 887
Bse8I GATNNNNATC 1 cut(s) 2737
BseAI TCCGGA 2 cut(s) 249, 1981
BseBI CCWGG 2 cut(s) 1221, 2667
BseDI CCNNGG 7 cut(s) 292, 471, 478, 1462, 2101, 2289, 3104
BseJI GATNNNNATC 1 cut(s) 2737
BseLI CCNNNNNNNGG 2 cut(s) 1654, 2557
BseMI GCAATG 2 cut(s) 623, 887
BseMII CTCAG 5 cut(s) 767, 1863, 2406, 2763, 2841
BseNI ACTGG 7 cut(s) 646, 915, 1569, 2270, 2318, 2488, 2651
BseRI GAGGAG 4 cut(s) 114, 117, 807, 2332
BseSI GKGCMC 1 cut(s) 1271
BseXI GCAGC 7 cut(s) 275, 923, 1106, 1840, 2154, 2650, 3185
BseYI CCCAGC 1 cut(s) 2556
BsgI GTGCAG 1 cut(s) 3061
BshFI GGCC 4 cut(s) 5, 937, 1224, 3103
BshNI GGYRCC 1 cut(s) 560
BshTI ACCGGT 1 cut(s) 185
BsiHKAI GWGCWC 7 cut(s) 1198, 1471, 1483, 1679, 1992, 2232, 3199
BsiSI CCGG 4 cut(s) 156, 186, 250, 1982
BslFI GGGAC 6 cut(s) 378, 1376, 1564, 2573, 2886, 3168
BslI CCNNNNNNNGG 2 cut(s) 1654, 2557
BsmAI GTCTC 1 cut(s) 2929
BsmFI GGGAC 6 cut(s) 378, 1376, 1564, 2573, 2886, 3168
BsnI GGCC 4 cut(s) 5, 937, 1224, 3103
Bso31I GGTCTC 1 cut(s) 2929
Bsp119I TTCGAA 1 cut(s) 1173
Bsp1286I GDGCHC 8 cut(s) 1198, 1271, 1471, 1483, 1679, 1992, 2232, 3199
Bsp13I TCCGGA 2 cut(s) 249, 1981
Bsp1407I TGTACA 2 cut(s) 2578, 2781
Bsp143I GATC 8 cut(s) 103, 455, 1816, 1905, 2441, 2524, 2732, 2857
BspACI CCGC 4 cut(s) 178, 244, 266, 965
BspANI GGCC 4 cut(s) 5, 937, 1224, 3103
BspCNI CTCAG 5 cut(s) 768, 1862, 2407, 2762, 2842
BspEI TCCGGA 2 cut(s) 249, 1981
BspHI TCATGA 4 cut(s) 12, 2790, 2841, 3028
BspLI GGNNCC 5 cut(s) 562, 2287, 2671, 2827, 2874
BspMAI CTGCAG 1 cut(s) 2187
BspMI ACCTGC 1 cut(s) 1154
BspPI GGATC 4 cut(s) 463, 1811, 2740, 2852
BspT104I TTCGAA 1 cut(s) 1173
BspT107I GGYRCC 1 cut(s) 560
BspTI CTTAAG 2 cut(s) 370, 2774
BspTNI GGTCTC 1 cut(s) 2929
BsrDI GCAATG 2 cut(s) 623, 887
BsrFI RCCGGY 1 cut(s) 185
BsrGI TGTACA 2 cut(s) 2578, 2781
BsrI ACTGG 7 cut(s) 646, 915, 1569, 2270, 2318, 2488, 2651
BssAI RCCGGY 1 cut(s) 185
BssECI CCNNGG 7 cut(s) 292, 471, 478, 1462, 2101, 2289, 3104
BssMI GATC 8 cut(s) 103, 455, 1816, 1905, 2441, 2524, 2732, 2857
BssNAI GTATAC 1 cut(s) 1239
BssSI CACGAG 1 cut(s) 1803
BssT1I CCWWGG 6 cut(s) 292, 478, 1462, 2101, 2289, 3104
Bst1107I GTATAC 1 cut(s) 1239
Bst2BI CACGAG 1 cut(s) 1803
Bst2UI CCWGG 2 cut(s) 1221, 2667
Bst6I CTCTTC 1 cut(s) 1827
BstAFI CTTAAG 2 cut(s) 370, 2774
BstAPI GCANNNNNTGC 1 cut(s) 2647
BstAUI TGTACA 2 cut(s) 2578, 2781
BstBI TTCGAA 1 cut(s) 1173
BstC8I GCNNGC 5 cut(s) 356, 1026, 1876, 2250, 2648
BstDEI CTNAG 8 cut(s) 221, 565, 585, 776, 1849, 2415, 2749, 2850
BstKTI GATC 8 cut(s) 106, 458, 1819, 1908, 2444, 2527, 2735, 2860
BstMAI GTCTC 1 cut(s) 2929
BstMBI GATC 8 cut(s) 103, 455, 1816, 1905, 2441, 2524, 2732, 2857
BstMWI GCNNNNNNNGC 6 cut(s) 119, 1797, 2207, 2553, 2638, 2647
BstNI CCWGG 2 cut(s) 1221, 2667
BstNSI RCATGY 2 cut(s) 1028, 2840
BstSCI CCNGG 2 cut(s) 1219, 2665
BstSFI CTRYAG 5 cut(s) 196, 1362, 1528, 1710, 2183
BstSLI GKGCMC 1 cut(s) 1271
BstV1I GCAGC 7 cut(s) 275, 923, 1106, 1840, 2154, 2650, 3185
BstV2I GAAGAC 3 cut(s) 293, 1286, 2171
BstXI CCANNNNNNTGG 1 cut(s) 2869
BstZ17I GTATAC 1 cut(s) 1239
BsuRI GGCC 4 cut(s) 5, 937, 1224, 3103
BtsI GCAGTG 2 cut(s) 2244, 2500
BtsIMutI CAGTG 8 cut(s) 205, 1198, 1576, 2244, 2481, 2500, 3021, 3055
BveI ACCTGC 1 cut(s) 1154
Cac8I GCNNGC 5 cut(s) 356, 1026, 1876, 2250, 2648
CaiI CAGNNNCTG 1 cut(s) 1856
CciI TCATGA 4 cut(s) 12, 2790, 2841, 3028
Cfr10I RCCGGY 1 cut(s) 185
Cfr13I GGNCC 5 cut(s) 289, 475, 608, 2286, 2873
CseI GACGC 1 cut(s) 380
CspAI ACCGGT 1 cut(s) 185
DdeI CTNAG 8 cut(s) 221, 565, 585, 776, 1849, 2415, 2749, 2850
DpnI GATC 8 cut(s) 105, 457, 1818, 1907, 2443, 2526, 2734, 2859
DpnII GATC 8 cut(s) 103, 455, 1816, 1905, 2441, 2524, 2732, 2857
DraI TTTAAA 1 cut(s) 717
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 1827
EarI CTCTTC 1 cut(s) 1827
Ecl136II GAGCTC 3 cut(s) 1469, 1677, 2230
Eco130I CCWWGG 6 cut(s) 292, 478, 1462, 2101, 2289, 3104
Eco147I AGGCCT 1 cut(s) 937
Eco24I GRGCYC 3 cut(s) 1471, 1679, 2232
Eco31I GGTCTC 1 cut(s) 2929
Eco47I GGWCC 5 cut(s) 289, 475, 608, 2286, 2873
Eco53kI GAGCTC 3 cut(s) 1469, 1677, 2230
Eco57I CTGAAG 4 cut(s) 63, 1473, 1638, 2388
EcoICRI GAGCTC 3 cut(s) 1469, 1677, 2230
EcoO109I RGGNCCY 1 cut(s) 2286
EcoRI GAATTC 2 cut(s) 1033, 2794
EcoRII CCWGG 2 cut(s) 1219, 2665
EcoT14I CCWWGG 6 cut(s) 292, 478, 1462, 2101, 2289, 3104
EcoT38I GRGCYC 3 cut(s) 1471, 1679, 2232
ErhI CCWWGG 6 cut(s) 292, 478, 1462, 2101, 2289, 3104
FaqI GGGAC 6 cut(s) 378, 1376, 1564, 2573, 2886, 3168
FauNDI CATATG 2 cut(s) 382, 1702
FbaI TGATCA 2 cut(s) 103, 2524
FblI GTMKAC 2 cut(s) 1238, 1713
Fnu4HI GCNGC 7 cut(s) 264, 912, 1120, 1854, 2168, 2639, 3174
FriOI GRGCYC 3 cut(s) 1471, 1679, 2232
Fsp4HI GCNGC 7 cut(s) 264, 912, 1120, 1854, 2168, 2639, 3174
FspBI CTAG 5 cut(s) 312, 546, 1047, 1116, 2802
GluI GCNGC 7 cut(s) 264, 912, 1120, 1854, 2168, 2639, 3174
GsaI CCCAGC 1 cut(s) 2560
GsuI CTGGAG 1 cut(s) 2335
HaeIII GGCC 4 cut(s) 5, 937, 1224, 3103
HapII CCGG 4 cut(s) 156, 186, 250, 1982
HgaI GACGC 1 cut(s) 380
HincII GTYRAC 1 cut(s) 2421
HindII GTYRAC 1 cut(s) 2421
HindIII AAGCTT 2 cut(s) 400, 2717
HinfI GANTC 8 cut(s) 428, 1205, 1706, 1978, 2417, 2461, 2694, 2705
HpaII CCGG 4 cut(s) 156, 186, 250, 1982
HphI GGTGA 4 cut(s) 167, 218, 1070, 1679
Hpy166II GTNNAC 9 cut(s) 175, 651, 1239, 1714, 2073, 2421, 2762, 2916, 3002
Hpy8I GTNNAC 9 cut(s) 175, 651, 1239, 1714, 2073, 2421, 2762, 2916, 3002
HpyAV CCTTC 8 cut(s) 642, 962, 1101, 1613, 2594, 2839, 3115, 3144
HpyCH4IV ACGT 1 cut(s) 49
HpyF10VI GCNNNNNNNGC 6 cut(s) 119, 1797, 2207, 2553, 2638, 2647
HpyF3I CTNAG 8 cut(s) 221, 565, 585, 776, 1849, 2415, 2749, 2850
HpySE526I ACGT 1 cut(s) 49
Kpn2I TCCGGA 2 cut(s) 249, 1981
Ksp22I TGATCA 2 cut(s) 103, 2524
Kzo9I GATC 8 cut(s) 103, 455, 1816, 1905, 2441, 2524, 2732, 2857
Lsp1109I GCAGC 7 cut(s) 275, 923, 1106, 1840, 2154, 2650, 3185
LweI GCATC 9 cut(s) 73, 852, 930, 970, 1276, 1800, 2001, 2053, 2368
MaeI CTAG 5 cut(s) 312, 546, 1047, 1116, 2802
MaeII ACGT 1 cut(s) 49
MalI GATC 8 cut(s) 105, 457, 1818, 1907, 2443, 2526, 2734, 2859
MboI GATC 8 cut(s) 103, 455, 1816, 1905, 2441, 2524, 2732, 2857
MfeI CAATTG 4 cut(s) 117, 234, 1104, 3158
MhlI GDGCHC 8 cut(s) 1198, 1271, 1471, 1483, 1679, 1992, 2232, 3199
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 2426
MmeI TCCRAC 8 cut(s) 433, 971, 1354, 1444, 1772, 1950, 2260, 2710
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 2 cut(s) 249, 1981
MroXI GAANNNNTTC 1 cut(s) 20
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 3 cut(s) 11, 1056, 2631
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 3 cut(s) 266, 1211, 1853
MspCI CTTAAG 2 cut(s) 370, 2774
MspI CCGG 4 cut(s) 156, 186, 250, 1982
MspR9I CCNGG 2 cut(s) 1221, 2667
MunI CAATTG 4 cut(s) 117, 234, 1104, 3158
MvaI CCWGG 2 cut(s) 1221, 2667
MwoI GCNNNNNNNGC 6 cut(s) 119, 1797, 2207, 2553, 2638, 2647
NdeI CATATG 2 cut(s) 382, 1702
NdeII GATC 8 cut(s) 103, 455, 1816, 1905, 2441, 2524, 2732, 2857
NlaIV GGNNCC 5 cut(s) 562, 2287, 2671, 2827, 2874
NmuCI GTSAC 5 cut(s) 43, 314, 1153, 1685, 2495
NspI RCATGY 2 cut(s) 1028, 2840
NspV TTCGAA 1 cut(s) 1173
PaeI GCATGC 1 cut(s) 1028
PagI TCATGA 4 cut(s) 12, 2790, 2841, 3028
PceI AGGCCT 1 cut(s) 937
PdmI GAANNNNTTC 1 cut(s) 20
PfeI GAWTC 7 cut(s) 428, 1205, 1706, 1978, 2461, 2694, 2705
PflFI GACNNNGTC 1 cut(s) 390
PinAI ACCGGT 1 cut(s) 185
PkrI GCNGC 7 cut(s) 265, 913, 1121, 1855, 2169, 2640, 3175
PleI GAGTC 1 cut(s) 2425
PpsI GAGTC 1 cut(s) 2425
PpuMI RGGWCCY 1 cut(s) 2286
Psp124BI GAGCTC 3 cut(s) 1471, 1679, 2232
Psp5II RGGWCCY 1 cut(s) 2286
Psp6I CCWGG 2 cut(s) 1219, 2665
PspFI CCCAGC 1 cut(s) 2556
PspGI CCWGG 2 cut(s) 1219, 2665
PspN4I GGNNCC 5 cut(s) 562, 2287, 2671, 2827, 2874
PspPI GGNCC 5 cut(s) 289, 475, 608, 2286, 2873
PspPPI RGGWCCY 1 cut(s) 2286
PsrI GAACNNNNNNTAC 4 cut(s) 632, 664, 2897, 2929
PstI CTGCAG 1 cut(s) 2187
PstNI CAGNNNCTG 1 cut(s) 1856
PsyI GACNNNGTC 1 cut(s) 390
PvuII CAGCTG 2 cut(s) 1211, 1853
RseI CAYNNNNRTG 3 cut(s) 11, 1056, 2631
SacI GAGCTC 3 cut(s) 1471, 1679, 2232
SatI GCNGC 7 cut(s) 264, 912, 1120, 1854, 2168, 2639, 3174
Sau3AI GATC 8 cut(s) 103, 455, 1816, 1905, 2441, 2524, 2732, 2857
Sau96I GGNCC 5 cut(s) 289, 475, 608, 2286, 2873
SchI GAGTC 1 cut(s) 2426
ScrFI CCNGG 2 cut(s) 1221, 2667
SduI GDGCHC 8 cut(s) 1198, 1271, 1471, 1483, 1679, 1992, 2232, 3199
SfaNI GCATC 9 cut(s) 73, 852, 930, 970, 1276, 1800, 2001, 2053, 2368
SfcI CTRYAG 5 cut(s) 196, 1362, 1528, 1710, 2183
SfuI TTCGAA 1 cut(s) 1173
SinI GGWCC 5 cut(s) 289, 475, 608, 2286, 2873
SmiMI CAYNNNNRTG 3 cut(s) 11, 1056, 2631
SmlI CTYRAG 7 cut(s) 87, 370, 524, 745, 1672, 2609, 2774
SmoI CTYRAG 7 cut(s) 87, 370, 524, 745, 1672, 2609, 2774
SpeI ACTAGT 2 cut(s) 545, 2801
SphI GCATGC 1 cut(s) 1028
SseBI AGGCCT 1 cut(s) 937
SsiI CCGC 4 cut(s) 178, 244, 266, 965
SspI AATATT 2 cut(s) 862, 3047
SspMI CTAG 5 cut(s) 312, 546, 1047, 1116, 2802
SstI GAGCTC 3 cut(s) 1471, 1679, 2232
StuI AGGCCT 1 cut(s) 937
StyD4I CCNGG 2 cut(s) 1219, 2665
StyI CCWWGG 6 cut(s) 292, 478, 1462, 2101, 2289, 3104
TaiI ACGT 1 cut(s) 52
TaqI TCGA 6 cut(s) 304, 1173, 1794, 1869, 2345, 2832
TatI WGTACW 5 cut(s) 320, 648, 2578, 2781, 2913
TfiI GAWTC 7 cut(s) 428, 1205, 1706, 1978, 2461, 2694, 2705
TscAI CASTG 8 cut(s) 205, 1198, 1576, 2244, 2488, 2500, 3021, 3062
TseFI GTSAC 5 cut(s) 43, 314, 1153, 1685, 2495
TseI GCWGC 7 cut(s) 263, 911, 1119, 1853, 2167, 2638, 3173
Tsp45I GTSAC 5 cut(s) 43, 314, 1153, 1685, 2495
TspGWI ACGGA 4 cut(s) 752, 1141, 1990, 2662
TspRI CASTG 8 cut(s) 205, 1198, 1576, 2244, 2488, 2500, 3021, 3062
Tth111I GACNNNGTC 1 cut(s) 390
Vha464I CTTAAG 2 cut(s) 370, 2774
VpaK11BI GGWCC 5 cut(s) 289, 475, 608, 2286, 2873
XapI RAATTY 9 cut(s) 18, 1033, 1111, 1134, 1412, 2172, 2379, 2536, 2794
XceI RCATGY 2 cut(s) 1028, 2840
XcmI CCANNNNNNNNNTGG 2 cut(s) 1891, 3177
XmiI GTMKAC 2 cut(s) 1238, 1713
XmnI GAANNNNTTC 1 cut(s) 20
XspI CTAG 5 cut(s) 312, 546, 1047, 1116, 2802
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.