RLG00000033531

Protein of unknown function (DUF2921)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000420
Physical Location & Seq
Reverse (-)
28561865 .. 28569126
7262 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033531

Sequence Viewer

Length: 3978 bp
ATGACCATTTTCATGAAAATTTTCACTTTCTTCTTGGTTTTTGTCACACGTTCGTTAAACTCTGTTTCGTTCTCCACTTCAGATGCTCAAGTGTCTTTTGCTGATCATTGTGCTTCAATTGCTCCTCACTACTCAACCCCACAAAGATATGCCGGACTTAATTCCTCTGTTCACCGCCTCACCGGTTTCTACTACACTGCTGGTGAAACAAACATTCTTAGTCAGAACTCAATTGTATTCCGCTTCCAGTATGATGGAGCAGCTGATGAACAAGGCTTGTTCATGGTCCAAGGAAGTCTTCGATTTCCTAGTGACAGTACATATTACCTTGTGGGAAACTCTACAAGTAGCTGGCAATCCACTGACTTGAGAGTTCCATATGGACAGCGTCCGAGAAGCTTTAGGCTAAATGGGGTCTGGTCAGAATCTTCTGGGGAGCTTTGTATGGTTGGATCAGGTTCTAATTCCTCGGACCAAGGTAATTTACTGACCTTTCCTGTGGTTCTTAAACTACATAAACTCAAGAGTTCAAGTAGTCTTACTAGTTTAATTAGTGGCACCTTAGAGAGCTTGGTAACTTCTAAGAATGAACCAAATTATTTTGGACCAATCTCTATATCATTGCTTCCTTCCATGAACTACCAGTACACTTTTGTTTCAAACAAATCTCATAACAGCTATTCGGATGGAAGTGTTGTTCCCCCAAGTTCTTTAAAAATAGGGAGATTTTGCAGGGTATTCTCAAGAGCAATACTATTCCGCGAGTTACAACTGAGATATTCAAGCCATTGTGGTTCTGCAAAGAACTGCTTTCCTCTTGCTCTGTCTGATTTGCCTCGCGTCATGTCGTTGAATAATATTGAGTGTTTGGAGGATAGAAAAACGTTGCGAGTTCTGGTAGATTTTGCAGCCAGTAGCATCAGTTACCAGAGGCCTTTCAATCCAACTACAACAATGGTTGCAGAAGGTTCATGGGATGCAAGGAAAAATCAGCTAAATGTTGTTGCTTGTCGGTTCGTGCATGCAAGGAATTCTTTGAGTCCTAGTCATGTGGGTGATTGTTCAACAAGATTGACCATGGGATTTCCTTCAATATGGACAATTGGAAATTCTAGCTGCATTGTGGGGAAAATTTGGAGCAACAAATCTGCGACAGAGGCAGGTTACTTCAAAAGCATCACATTTGACAGTGCTCAAAGTGATTCATCTGTATTTCAAGGCCAGAGATATGAGTATACCAAAATAGACAAAGCAACAAAGTTGTGCCCAAGAAAGAAGACTGATGCTGATGACAAGAACAACATTTATCCAAATCCTTTTTCTTATTACATGGGATTGGATATTACAGCCAACAGTTCTACAGGAGAAGTTGGATGGGGCTATTCTGTTCCTCTCTCTGTTGATAACCAATTCTTCCAACCGTATTGGTACTCTCTGGAAGATGGAACTGAAGAATACCTTGTAGCTCCTGTGAGTGCTCCTGGGAGCGATAGAAAAAACAATAGCAACAGCTACAACATCAGCTACAGAATAAGCATCAAAGCAGTACATGAGGTTAAGTTTCCCAGTGGAGTGTCCCAACTTAATTCATTATATGAGATGGGGATTTTTGCTGAAGGGATATATGATGGCAGAGATGGAAGCCTGTGCATGGTAGGTTGCCGATATTTGAGCTCAAACAGTCACCAACAGCCAACATATGATTCTGTAGACTGTGAGATTGTTGTCAATGTTCAGTTTCCTCCAACAAATCCAAACAGTACCTATTCGAGTTTTATCAAGGGAAGCATCGAAAGCACACGAAAAATGTCTGATCCTCTTCATTTTGAGCGATTGGATTTGACCTCAGCTGCTGGTGACATGGTTGAAGCAAGCCAATCCCTTTGGAGAATGGATGTGGAGATCATATTGTTAACAATGTCAGCAAAATCAGCAAATGGAAACCAAAAGGAGCTCTGGGCAGTGGAGAAGCTGGCTCTCTTTGTGGCTTTACCAGTATATGTTGGAGGTTCCTTGGTTGCTTTGCGTCTGATGAACTGGAGGAGGAACAATACTGCTGTGATGGTTTCGAGTGGTCAAAAGCATCCCATTTTGGCTGTTCAAATTTCTTATGCTGACCATTGTGCTTCAGTTGTTCCCGAGTCAACCGAAAAGATAATTGCAGGATCTCGGTTTGTGCATCTGATTCACACAGGTTACTACTACATTGGTGGTGGCAGTGACATTCTTAATGTATACTCGCCAGATGATCAGCTGCAAACCCTGCCGGTTCAAACATTTCCTGGTTCGGTTTCCTTTAGACTCAATGGATTCTGGTCAGAATCTTCTGGGAAGCTTTGCATGGTTGGATCATCGTCCAGTCACTCGCAACAAGGTAATCCTCTTGTTCTTAAGCTGCATAATCTCATGAATTCCACTTGTGTTACAAGTTTGATTAGTGGAACCTTCGAGAATGTCATGATATCTGAGGATGATCCCAAGTATTTGGGACCAATCTCTATTTTGCTGTTTCCTCGCATGAACTACGAGTACACTTTGGTCTCAACTAATTCTAAAAACAGTTGCTCTAGTGGAAGTGAAGATACCCCAAGCTCTATACAAGTAGAGAGATTTTGTTCACTACTATCATCAGTAGTACTTAATCATGACTTTCAACGCAAATATTCAAGCCACTGTGTTACTGCAAAGAACTGCACTCCAATTTCTATGGCTGATTGGCCTCAGGCTGTATCGTTTAAGGGCATCTCATGTTCAGAGGATAAACAAAGGGTCAGAGTTCTGGTAGAATTTGTAGACAGTTGGCCAAACTGGCATCCAAGGCTCTTCAATGCTAGTAAGAATTTAGTTGGTGAAGGCTCATGGGATGCAGAGAAGAATCAGCTATGTATCATTGCTTGTCGAATCTTGACTTCAACAAAGTCTTTGAATAATACTAATGTGGGTGATTGTTCAACCAGATTGAGCTTGAGATTTCCTGCAACAGGAACAATTGGAAGTACTAGCAGCATTGTGGGGCATATGTGGAGCACCAAAACGGTGACAGAGTTGGGCTACTTTGAAAAGATCACATTTGCAAGTCGGGAATGTGATCGTCAAGAGTGTCTACTTCCAGGCCAGACATACAAGTATACTAAAATGGAGAAAGGAACAACGTTGTGCTCGAGAGAGAAGACAGCAAATGACCCTTCCAGCATTTACCCAAATCTGTTTTTGCATGTCATGAGTTTCTTTATGCATGCCGAAAATTCTGAAGGGCAAGTTGGTAGTGGCACTTGTGTTCCTCTCTCTGTTGACAATCAATTTTACAGCACTGATGATTCAACTGTAAGCAATCAAGAATACTCTGGGACTCCGCTGAGTGACAGCTACAACCACAGCAGCTCCTACAATATTAGCTACAAAATATACCTCCAACTACAATCTAATCCACAGTTGGGCGAACAGTCAGTTAGATATGAAATGCACATCTCTGCTGAAGGGATCTATGATGATACAAAAGGAAGCCTGTGCATCGTAGGCTGTCAAGATCTAGGCCCAATGGCCATGTTCACGCATAATATCAATCATCGAAATGTGTACCTTGGAGGAGGTGGATGGCTGGAATTTAATGAGATGGTCATAAGGGTGACAACATTTATAGCTTTCTTTATACAATTCCACCTTATGAAGCTAACTTGGTCAGCAAAATCAGGAAATGGAACCCGGAAGGATCTCTGGGTCATGGAAAAGAAGCCTCTGTTTGTGGCTTTACGTGTGTATGCTGTAGGGGCTTTAGCTGCTTTGTTTCTGCATAGACAGAACCTGAGGAAGAGGGACGATGGCATTGTGCTGCATTATGTGGAAGCTGGTTGGGTTTGGACTTCGGAGTGGCATAATTATTACATGCCCATTCGAAGCAAGGATTTGAGCTATGCGGCTAGGAAAGGCTCTGGGTCAGATGGCTTCAGCTATCTGGCTTTACTTACCGTTGTGGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
DUF2921

Protein Analysis

1326

Amino Acids

147.34

Weight (kDa)

7.49

Isoelectric Point (pI)

41.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2921_N PF25333 66 - 205 2.9e-17 DUF2921 lipocalin-like domain
DUF2921_N PF25333 226 - 394 1e-25 DUF2921 lipocalin-like domain
DUF2921_N PF25333 420 - 613 1.5e-32 DUF2921 lipocalin-like domain
DUF2921_N PF25333 755 - 833 2.4e-10 DUF2921 lipocalin-like domain
DUF2921_N PF25333 870 - 1023 1.9e-24 DUF2921 lipocalin-like domain
DUF2921_N PF25333 1056 - 1178 1.3e-12 DUF2921 lipocalin-like domain
DUF2921 PF11145 1168 - 1270 9.2e-06 Transmembrane E3 ligase/DUF2921 transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21700
fragaria_vesca FvH4_1g10360 FvH4_1g10760 FvH4_2g30370 FvH4_3g09240 FvH4_3g20050 FvH4_3g20280 FvH4_3g20290 FvH4_3g20291 FvH4_3g20340 FvH4_3g20350 FvH4_3g20351 FvH4_3g20380 FvH4_3g20390 FvH4_5g13190 FvH4_6g20070 FvH4_6g42890
malus_domestica MD03G1227000.v1.1 MD10G1294900.v1.1 MD11G1246300.v1.1 MD11G1246400.v1.1 MD11G1246500.v1.1 MD11G1246600.v1.1
prunus_persica Prupe.4G047300_v2.0.a1 Prupe.4G181700_v2.0.a1 Prupe.4G181900_v2.0.a1 Prupe.4G182000_v2.0.a1 Prupe.4G182200_v2.0.a1
pyrus_communis pycom03g17470 pycom10g24670 pycom11g21640
rosa_chinensis RchiOBHm_Chr5g0008381 RchiOBHm_Chr5g0008391 RchiOBHm_Chr5g0033881 RchiOBHm_Chr5g0034171 RchiOBHm_Chr5g0034181 RchiOBHm_Chr5g0034191 RchiOBHm_Chr5g0034201 RchiOBHm_Chr5g0034211 RchiOBHm_Chr5g0034221 RchiOBHm_Chr5g0034231 RchiOBHm_Chr5g0034271 RchiOBHm_Chr5g0034281
rosa_laevigata RLG00000031566 RLG00000031567 RLG00000033506 RLG00000033529 RLG00000033530 RLG00000033531
rosa_multiflora Rmu_co8445509.1_g000001 Rmu_co8470615.1_g000001 Rmu_sc0008805.1_g000003 Rmu_sc0008805.1_g000004 Rmu_ssc0000128.1_g000029 Rmu_ssc0000128.1_g000031 Rmu_ssc0000128.1_g000032 Rmu_ssc0000388.1_g000062 Rmu_ssc0000388.1_g000063
rosa_roxburghii Rroxscaffold_1G00046010 Rroxscaffold_1G00046020 Rroxscaffold_1G00046030 Rroxscaffold_1G00046040 Rroxscaffold_1G00046060 Rroxscaffold_1G00046070 Rroxscaffold_1G00046080 Rroxscaffold_1G00046090
rosa_rugosa Rorug04G0435500 Rorug05G0142900 Rorug05G0144600 Rorug05G0144600 Rorug05G0144700 Rorug05G0144800
rosa_samantha Rh5AG233800 Rh5AG235900 Rh5AG236000 Rh5AG236100 Rh5AG236200 Rh5AG236300 Rh5AG236500 Rh5BG061200 Rh5BG061300 Rh5BG234300 Rh5BG236600 Rh5BG236800 Rh5CG071200 Rh5DG060000 Rh5DG060100 Rh5DG241300 Rh5DG243700 Rh5DG244000 Rh5DG244100
rosa_wichuraiana Rw3G018950 Rw5G005680 Rw5G021330 Rw5G021340 Rw5G021350 Rw5G021550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1151
AccB1I GGYRCC 1 cut(s) 557
AccI GTMKAC 6 cut(s) 1235, 1710, 2235, 2793, 3103, 3128
AccII CGCG 2 cut(s) 762, 840
AciI CCGC 5 cut(s) 175, 241, 760, 3353, 3914
AclI AACGTT 2 cut(s) 884, 3152
AclWI GGATC 7 cut(s) 460, 1808, 2173, 2356, 2468, 3488, 3717
AcoI YGGCCR 2 cut(s) 2801, 3540
AcuI CTGAAG 7 cut(s) 63, 1470, 1635, 2112, 3270, 3495, 3928
AfaI GTAC 9 cut(s) 319, 647, 1430, 1548, 1762, 2531, 2637, 2998, 3578
AfiI CCNNNNNNNGG 3 cut(s) 1651, 2981, 3434
AflII CTTAAG 1 cut(s) 2390
AflIII ACRYGT 2 cut(s) 47, 3751
AgeI ACCGGT 1 cut(s) 182
AhlI ACTAGT 1 cut(s) 542
AjnI CCWGG 3 cut(s) 1480, 2281, 3109
AjuI GAANNNNNNNTTGG 8 cut(s) 282, 314, 1303, 1335, 1687, 1719, 3243, 3275
AloI GAACNNNNNNTCC 2 cut(s) 681, 713
Alw21I GWGCWC 6 cut(s) 1195, 1480, 1676, 1956, 3029, 3161
Alw26I GTCTC 1 cut(s) 2545
AlwI GGATC 7 cut(s) 460, 1808, 2173, 2356, 2468, 3488, 3717
AlwNI CAGNNNCTG 2 cut(s) 1853, 3802
Ama87I CYCGRG 2 cut(s) 2138, 3160
AoxI GGCC 7 cut(s) 932, 1219, 2717, 2801, 3112, 3532, 3540
AsiGI ACCGGT 1 cut(s) 182
Asp700I GAANNNNTTC 1 cut(s) 20
AspS9I GGNCC 5 cut(s) 286, 472, 605, 2489, 3533
AsuC2I CCSGG 1 cut(s) 3703
AsuII TTCGAA 1 cut(s) 3892
AvaI CYCGRG 2 cut(s) 2138, 3160
AvaII GGWCC 4 cut(s) 286, 472, 605, 2489
AxyI CCTNAGG 2 cut(s) 2721, 3803
BaeGI GKGCMC 1 cut(s) 1268
BalI TGGCCA 2 cut(s) 2803, 3542
BanI GGYRCC 1 cut(s) 557
BanII GRGCYC 2 cut(s) 1676, 1956
BarI GAAGNNNNNNTAC 2 cut(s) 2566, 2598
BbsI GAAGAC 3 cut(s) 290, 1283, 3176
Bbv12I GWGCWC 6 cut(s) 1195, 1480, 1676, 1956, 3029, 3161
BbvCI CCTCAGC 1 cut(s) 1846
BciT130I CCWGG 3 cut(s) 1482, 2283, 3111
BclI TGATCA 2 cut(s) 103, 2248
BcnI CCSGG 1 cut(s) 3703
BcoDI GTCTC 1 cut(s) 2545
BcuI ACTAGT 1 cut(s) 542
BfaI CTAG 9 cut(s) 309, 543, 1044, 1113, 2568, 2832, 3000, 3530, 3918
BfmI CTRYAG 4 cut(s) 1359, 1525, 1707, 3762
BfrI CTTAAG 1 cut(s) 2390
BfuAI ACCTGC 1 cut(s) 1151
BglI GCCNNNNNGGC 1 cut(s) 2809
BglII AGATCT 1 cut(s) 3526
BmcAI AGTACT 2 cut(s) 2637, 2998
Bme1390I CCNGG 4 cut(s) 1482, 2283, 3111, 3703
Bme18I GGWCC 4 cut(s) 286, 472, 605, 2489
BmeT110I CYCGRG 2 cut(s) 2138, 3160
BmgT120I GGNCC 5 cut(s) 286, 472, 605, 2489, 3533
BmiI GGNNCC 5 cut(s) 559, 2011, 2443, 2490, 3700
BmrFI CCNGG 4 cut(s) 1482, 2283, 3111, 3703
BmrI ACTGGG 1 cut(s) 1560
BmuI ACTGGG 1 cut(s) 1560
BpiI GAAGAC 3 cut(s) 290, 1283, 3176
BpmI CTGGAG 1 cut(s) 2059
Bpu10I CCTNAGC 1 cut(s) 1846
Bpu14I TTCGAA 1 cut(s) 3892
BpuEI CTTGAG 5 cut(s) 72, 388, 506, 727, 2986
BpuMI CCSGG 1 cut(s) 3703
BsaAI YACGTR 1 cut(s) 3752
BsaI GGTCTC 1 cut(s) 2545
BsaJI CCNNGG 8 cut(s) 289, 468, 475, 1077, 1481, 2013, 2816, 3580
BsaWI WCCGGW 1 cut(s) 182
BsaXI ACNNNNNCTCC 2 cut(s) 3365, 3395
Bsc4I CCNNNNNNNGG 3 cut(s) 1651, 2981, 3434
Bse118I RCCGGY 2 cut(s) 182, 2266
Bse1I ACTGG 8 cut(s) 247, 643, 912, 1566, 1994, 2042, 2358, 2813
Bse21I CCTNAGG 2 cut(s) 2721, 3803
Bse3DI GCAATG 2 cut(s) 620, 2889
BseBI CCWGG 3 cut(s) 1482, 2283, 3111
BseDI CCNNGG 8 cut(s) 289, 468, 475, 1077, 1481, 2013, 2816, 3580
BseGI GGATG 9 cut(s) 691, 982, 1379, 1900, 2083, 2476, 2812, 2869, 3599
BseLI CCNNNNNNNGG 3 cut(s) 1651, 2981, 3434
BseMI GCAATG 2 cut(s) 620, 2889
BseMII CTCAG 6 cut(s) 764, 1860, 2457, 2735, 3347, 3794
BseNI ACTGG 8 cut(s) 247, 643, 912, 1566, 1994, 2042, 2358, 2813
BseRI GAGGAG 3 cut(s) 114, 2056, 3600
BseSI GKGCMC 1 cut(s) 1268
BsgI GTGCAG 1 cut(s) 2677
Bsh1236I CGCG 2 cut(s) 762, 840
BshFI GGCC 7 cut(s) 934, 1221, 2719, 2803, 3114, 3534, 3542
BshNI GGYRCC 1 cut(s) 557
BshTI ACCGGT 1 cut(s) 182
BsiHKAI GWGCWC 6 cut(s) 1195, 1480, 1676, 1956, 3029, 3161
BsiHKCI CYCGRG 2 cut(s) 2138, 3160
BsiSI CCGG 4 cut(s) 153, 183, 2267, 3703
BslFI GGGAC 4 cut(s) 1561, 2502, 3361, 3827
BslI CCNNNNNNNGG 3 cut(s) 1651, 2981, 3434
BsmAI GTCTC 1 cut(s) 2545
BsmFI GGGAC 4 cut(s) 1561, 2502, 3361, 3827
BsnI GGCC 7 cut(s) 934, 1221, 2719, 2803, 3114, 3534, 3542
Bso31I GGTCTC 1 cut(s) 2545
BsoBI CYCGRG 2 cut(s) 2138, 3160
Bsp119I TTCGAA 1 cut(s) 3892
Bsp1286I GDGCHC 7 cut(s) 1195, 1268, 1480, 1676, 1956, 3029, 3161
Bsp19I CCATGG 1 cut(s) 1077
BspACI CCGC 5 cut(s) 175, 241, 760, 3353, 3914
BspANI GGCC 7 cut(s) 934, 1221, 2719, 2803, 3114, 3534, 3542
BspCNI CTCAG 6 cut(s) 765, 1859, 2458, 2734, 3348, 3795
BspFNI CGCG 2 cut(s) 762, 840
BspHI TCATGA 5 cut(s) 12, 2406, 2457, 2644, 3219
BspLI GGNNCC 5 cut(s) 559, 2011, 2443, 2490, 3700
BspMI ACCTGC 1 cut(s) 1151
BspPI GGATC 7 cut(s) 460, 1808, 2173, 2356, 2468, 3488, 3717
BspQI GCTCTTC 1 cut(s) 2828
BspT104I TTCGAA 1 cut(s) 3892
BspT107I GGYRCC 1 cut(s) 557
BspTI CTTAAG 1 cut(s) 2390
BspTNI GGTCTC 1 cut(s) 2545
BsrDI GCAATG 2 cut(s) 620, 2889
BsrFI RCCGGY 2 cut(s) 182, 2266
BsrI ACTGG 8 cut(s) 247, 643, 912, 1566, 1994, 2042, 2358, 2813
BssAI RCCGGY 2 cut(s) 182, 2266
BssECI CCNNGG 8 cut(s) 289, 468, 475, 1077, 1481, 2013, 2816, 3580
BssNAI GTATAC 3 cut(s) 1236, 2236, 3129
BssT1I CCWWGG 6 cut(s) 289, 475, 1077, 2013, 2816, 3580
Bst1107I GTATAC 3 cut(s) 1236, 2236, 3129
Bst2UI CCWGG 3 cut(s) 1482, 2283, 3111
Bst6I CTCTTC 3 cut(s) 1824, 2828, 3803
BstAFI CTTAAG 1 cut(s) 2390
BstAPI GCANNNNNTGC 1 cut(s) 2263
BstBAI YACGTR 1 cut(s) 3752
BstBI TTCGAA 1 cut(s) 3892
BstC8I GCNNGC 5 cut(s) 353, 1023, 1873, 1974, 3237
BstDEI CTNAG 9 cut(s) 218, 562, 582, 773, 1846, 2466, 2721, 3356, 3803
BstDSI CCRYGG 1 cut(s) 1077
BstENI CCTNNNNNAGG 1 cut(s) 2979
BstF5I GGATG 9 cut(s) 691, 982, 1379, 1900, 2083, 2476, 2812, 2869, 3599
BstFNI CGCG 2 cut(s) 762, 840
BstMAI GTCTC 1 cut(s) 2545
BstNI CCWGG 3 cut(s) 1482, 2283, 3111
BstNSI RCATGY 4 cut(s) 1025, 3218, 3239, 3886
BstSCI CCNGG 4 cut(s) 1480, 2281, 3109, 3701
BstSFI CTRYAG 4 cut(s) 1359, 1525, 1707, 3762
BstSLI GKGCMC 1 cut(s) 1268
BstUI CGCG 2 cut(s) 762, 840
BstV2I GAAGAC 3 cut(s) 290, 1283, 3176
BstX2I RGATCY 4 cut(s) 2165, 3480, 3526, 3709
BstXI CCANNNNNNTGG 2 cut(s) 254, 2485
BstYI RGATCY 4 cut(s) 2165, 3480, 3526, 3709
BstZ17I GTATAC 3 cut(s) 1236, 2236, 3129
Bsu36I CCTNAGG 2 cut(s) 2721, 3803
BsuRI GGCC 7 cut(s) 934, 1221, 2719, 2803, 3114, 3534, 3542
BtgI CCRYGG 1 cut(s) 1077
BtsCI GGATG 9 cut(s) 691, 982, 1379, 1900, 2083, 2476, 2812, 2869, 3599
BtsI GCAGTG 3 cut(s) 195, 1968, 2224
BtsIMutI CAGTG 8 cut(s) 195, 360, 1195, 1573, 1968, 2224, 2671, 3309
BveI ACCTGC 1 cut(s) 1151
Cac8I GCNNGC 5 cut(s) 353, 1023, 1873, 1974, 3237
CaiI CAGNNNCTG 2 cut(s) 1853, 3802
CciI TCATGA 5 cut(s) 12, 2406, 2457, 2644, 3219
Cfr10I RCCGGY 2 cut(s) 182, 2266
Cfr13I GGNCC 5 cut(s) 286, 472, 605, 2489, 3533
CseI GACGC 3 cut(s) 377, 829, 2015
Csp6I GTAC 9 cut(s) 318, 646, 1429, 1547, 1761, 2530, 2636, 2997, 3577
CspAI ACCGGT 1 cut(s) 182
CviQI GTAC 9 cut(s) 318, 646, 1429, 1547, 1761, 2530, 2636, 2997, 3577
DdeI CTNAG 9 cut(s) 218, 562, 582, 773, 1846, 2466, 2721, 3356, 3803
DraI TTTAAA 1 cut(s) 714
EaeI YGGCCR 2 cut(s) 2801, 3540
Eam1104I CTCTTC 3 cut(s) 1824, 2828, 3803
EarI CTCTTC 3 cut(s) 1824, 2828, 3803
Ecl136II GAGCTC 2 cut(s) 1674, 1954
Eco130I CCWWGG 6 cut(s) 289, 475, 1077, 2013, 2816, 3580
Eco147I AGGCCT 1 cut(s) 934
Eco24I GRGCYC 2 cut(s) 1676, 1956
Eco31I GGTCTC 1 cut(s) 2545
Eco32I GATATC 1 cut(s) 2463
Eco47I GGWCC 4 cut(s) 286, 472, 605, 2489
Eco53kI GAGCTC 2 cut(s) 1674, 1954
Eco57I CTGAAG 7 cut(s) 63, 1470, 1635, 2112, 3270, 3495, 3928
Eco81I CCTNAGG 2 cut(s) 2721, 3803
Eco88I CYCGRG 2 cut(s) 2138, 3160
EcoICRI GAGCTC 2 cut(s) 1674, 1954
EcoNI CCTNNNNNAGG 1 cut(s) 2979
EcoRI GAATTC 2 cut(s) 1030, 2410
EcoRII CCWGG 3 cut(s) 1480, 2281, 3109
EcoRV GATATC 1 cut(s) 2463
EcoT14I CCWWGG 6 cut(s) 289, 475, 1077, 2013, 2816, 3580
EcoT22I ATGCAT 1 cut(s) 3237
EcoT38I GRGCYC 2 cut(s) 1676, 1956
ErhI CCWWGG 6 cut(s) 289, 475, 1077, 2013, 2816, 3580
FalI AAGNNNNNCTT 8 cut(s) 282, 314, 794, 826, 1018, 1050, 1443, 1475
FaqI GGGAC 4 cut(s) 1561, 2502, 3361, 3827
FauNDI CATATG 3 cut(s) 379, 1699, 3018
FbaI TGATCA 2 cut(s) 103, 2248
FblI GTMKAC 6 cut(s) 1235, 1710, 2235, 2793, 3103, 3128
FokI GGATG 9 cut(s) 698, 989, 1386, 1907, 2070, 2483, 2799, 2876, 3606
FriOI GRGCYC 2 cut(s) 1676, 1956
FspBI CTAG 9 cut(s) 309, 543, 1044, 1113, 2568, 2832, 3000, 3530, 3918
GsuI CTGGAG 1 cut(s) 2059
HaeIII GGCC 7 cut(s) 934, 1221, 2719, 2803, 3114, 3534, 3542
HapII CCGG 4 cut(s) 153, 183, 2267, 3703
HgaI GACGC 3 cut(s) 377, 829, 2015
HincII GTYRAC 3 cut(s) 1914, 2145, 3292
HindII GTYRAC 3 cut(s) 1914, 2145, 3292
HindIII AAGCTT 2 cut(s) 397, 2333
HpaI GTTAAC 1 cut(s) 1914
HpaII CCGG 4 cut(s) 153, 183, 2267, 3703
HpyCH4IV ACGT 4 cut(s) 49, 884, 3152, 3751
HpyF3I CTNAG 9 cut(s) 218, 562, 582, 773, 1846, 2466, 2721, 3356, 3803
HpySE526I ACGT 4 cut(s) 49, 884, 3152, 3751
Ksp22I TGATCA 2 cut(s) 103, 2248
KspAI GTTAAC 1 cut(s) 1914
LguI GCTCTTC 1 cut(s) 2828
MaeI CTAG 9 cut(s) 309, 543, 1044, 1113, 2568, 2832, 3000, 3530, 3918
MaeII ACGT 4 cut(s) 49, 884, 3152, 3751
MfeI CAATTG 4 cut(s) 117, 231, 1101, 2988
MflI RGATCY 4 cut(s) 2165, 3480, 3526, 3709
MhlI GDGCHC 7 cut(s) 1195, 1268, 1480, 1676, 1956, 3029, 3161
MlsI TGGCCA 2 cut(s) 2803, 3542
MluNI TGGCCA 2 cut(s) 2803, 3542
MlyI GAGTC 4 cut(s) 1048, 2150, 2295, 3343
MmeI TCCRAC 8 cut(s) 430, 968, 1351, 1441, 1769, 1984, 2326, 3436
Mox20I TGGCCA 2 cut(s) 2803, 3542
Mph1103I ATGCAT 1 cut(s) 3237
MroXI GAANNNNTTC 1 cut(s) 20
MscI TGGCCA 2 cut(s) 2803, 3542
MslI CAYNNNNRTG 4 cut(s) 11, 1053, 3570, 3623
Msp20I TGGCCA 2 cut(s) 2803, 3542
MspA1I CMGCKG 4 cut(s) 263, 1850, 2254, 3355
MspCI CTTAAG 1 cut(s) 2390
MspI CCGG 4 cut(s) 153, 183, 2267, 3703
MspR9I CCNGG 4 cut(s) 1482, 2283, 3111, 3703
MunI CAATTG 4 cut(s) 117, 231, 1101, 2988
MvaI CCWGG 3 cut(s) 1482, 2283, 3111
MvnI CGCG 2 cut(s) 762, 840
NciI CCSGG 1 cut(s) 3703
NcoI CCATGG 1 cut(s) 1077
NdeI CATATG 3 cut(s) 379, 1699, 3018
NlaIV GGNNCC 5 cut(s) 559, 2011, 2443, 2490, 3700
NmuCI GTSAC 9 cut(s) 43, 311, 1682, 1856, 2219, 2360, 3037, 3359, 3625
NsiI ATGCAT 1 cut(s) 3237
NspI RCATGY 4 cut(s) 1025, 3218, 3239, 3886
NspV TTCGAA 1 cut(s) 3892
PaeI GCATGC 2 cut(s) 1025, 3239
PaeR7I CTCGAG 1 cut(s) 3160
PagI TCATGA 5 cut(s) 12, 2406, 2457, 2644, 3219
PceI AGGCCT 1 cut(s) 934
PciSI GCTCTTC 1 cut(s) 2828
PcsI WCGNNNNNNNCGW 1 cut(s) 3158
PdmI GAANNNNTTC 1 cut(s) 20
PfeI GAWTC 9 cut(s) 425, 1202, 1703, 2185, 2310, 2321, 2875, 2901, 3317
PflFI GACNNNGTC 1 cut(s) 387
PinAI ACCGGT 1 cut(s) 182
PleI GAGTC 4 cut(s) 1047, 2149, 2295, 3343
PpsI GAGTC 4 cut(s) 1047, 2149, 2295, 3343
Ppu21I YACGTR 1 cut(s) 3752
Psp124BI GAGCTC 2 cut(s) 1676, 1956
Psp1406I AACGTT 2 cut(s) 884, 3152
Psp6I CCWGG 3 cut(s) 1480, 2281, 3109
PspGI CCWGG 3 cut(s) 1480, 2281, 3109
PspN4I GGNNCC 5 cut(s) 559, 2011, 2443, 2490, 3700
PspPI GGNCC 5 cut(s) 286, 472, 605, 2489, 3533
PsrI GAACNNNNNNTAC 4 cut(s) 629, 661, 2513, 2545
PstNI CAGNNNCTG 2 cut(s) 1853, 3802
PsuI RGATCY 4 cut(s) 2165, 3480, 3526, 3709
PsyI GACNNNGTC 1 cut(s) 387
PvuII CAGCTG 3 cut(s) 263, 1850, 2254
RsaI GTAC 9 cut(s) 319, 647, 1430, 1548, 1762, 2531, 2637, 2998, 3578
RsaNI GTAC 9 cut(s) 318, 646, 1429, 1547, 1761, 2530, 2636, 2997, 3577
RseI CAYNNNNRTG 4 cut(s) 11, 1053, 3570, 3623
SacI GAGCTC 2 cut(s) 1676, 1956
SapI GCTCTTC 1 cut(s) 2828
Sau96I GGNCC 5 cut(s) 286, 472, 605, 2489, 3533
ScaI AGTACT 2 cut(s) 2637, 2998
SchI GAGTC 4 cut(s) 1048, 2150, 2295, 3343
ScrFI CCNGG 4 cut(s) 1482, 2283, 3111, 3703
SduI GDGCHC 7 cut(s) 1195, 1268, 1480, 1676, 1956, 3029, 3161
SfcI CTRYAG 4 cut(s) 1359, 1525, 1707, 3762
Sfr274I CTCGAG 1 cut(s) 3160
SfuI TTCGAA 1 cut(s) 3892
SinI GGWCC 4 cut(s) 286, 472, 605, 2489
SlaI CTCGAG 1 cut(s) 3160
SmiMI CAYNNNNRTG 4 cut(s) 11, 1053, 3570, 3623
SmlI CTYRAG 7 cut(s) 87, 367, 521, 742, 2390, 2965, 3160
SmoI CTYRAG 7 cut(s) 87, 367, 521, 742, 2390, 2965, 3160
SpeI ACTAGT 1 cut(s) 542
SphI GCATGC 2 cut(s) 1025, 3239
SseBI AGGCCT 1 cut(s) 934
SsiI CCGC 5 cut(s) 175, 241, 760, 3353, 3914
SspI AATATT 3 cut(s) 859, 2663, 3391
SspMI CTAG 9 cut(s) 309, 543, 1044, 1113, 2568, 2832, 3000, 3530, 3918
SstI GAGCTC 2 cut(s) 1676, 1956
StuI AGGCCT 1 cut(s) 934
StyD4I CCNGG 4 cut(s) 1480, 2281, 3109, 3701
StyI CCWWGG 6 cut(s) 289, 475, 1077, 2013, 2816, 3580
TaiI ACGT 4 cut(s) 52, 887, 3155, 3754
TaqI TCGA 9 cut(s) 301, 1769, 1791, 2069, 2448, 2899, 3161, 3568, 3892
TatI WGTACW 6 cut(s) 317, 645, 1546, 2529, 2635, 2996
TauI GCSGC 1 cut(s) 3917
TfiI GAWTC 9 cut(s) 425, 1202, 1703, 2185, 2310, 2321, 2875, 2901, 3317
TscAI CASTG 8 cut(s) 202, 367, 1195, 1573, 1968, 2224, 2678, 3316
TseFI GTSAC 9 cut(s) 43, 311, 1682, 1856, 2219, 2360, 3037, 3359, 3625
Tsp45I GTSAC 9 cut(s) 43, 311, 1682, 1856, 2219, 2360, 3037, 3359, 3625
TspRI CASTG 8 cut(s) 202, 367, 1195, 1573, 1968, 2224, 2678, 3316
Tth111I GACNNNGTC 1 cut(s) 387
Vha464I CTTAAG 1 cut(s) 2390
VpaK11BI GGWCC 4 cut(s) 286, 472, 605, 2489
XagI CCTNNNNNAGG 1 cut(s) 2979
XceI RCATGY 4 cut(s) 1025, 3218, 3239, 3886
XhoI CTCGAG 1 cut(s) 3160
XmiI GTMKAC 6 cut(s) 1235, 1710, 2235, 2793, 3103, 3128
XmnI GAANNNNTTC 1 cut(s) 20
XspI CTAG 9 cut(s) 309, 543, 1044, 1113, 2568, 2832, 3000, 3530, 3918
ZrmI AGTACT 2 cut(s) 2637, 2998
Zsp2I ATGCAT 1 cut(s) 3237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.