Rh5DG244100

Protein of unknown function (DUF2921)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000420
Physical Location & Seq
Reverse (-)
27913693 .. 27917330
3638 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG244100.1

Sequence Viewer

Length: 2685 bp
ATGCTGGAAGATGCTCAAGTGTATTTTGCTGATCATTGTGCTTCAATTGCTCCTCCTCACTACTCAACCCCACAAAGATATGCCGGACTTAATTCCTCTGTTCACCGCCTTACCGGTTTCTACTACAGTGCTGGTGAAACTAACATTCTTAGTCAGAACTCAATTGTATTCCGCTTCCGGAATGATGGAGTAGCGGATGAACAAGGCTTGTTCATGGTCCAAGGAAGTCTTCGATTTCCTAGTGACAGTACATATAACCTTGTGGGAAACTCTACAAGGAGCTGGCAATCCACCGACTTGAGAGTTCCATATGGACAGCGTCCCAGAAGCTTTAGGCTAAATGGGGTCTGGTCAGAATCTTCTGGGGAGCTTTGTATGGTTGGATCAGGTTCTAATTCCTCGGACCAAGGTAATTTACTGACCTTTCCTGTGGTTCTTAAACTACATAAACTCAAGAGTTCAAGTAGTCTTACTAGTTTAATTAGTGGCAGCTTAGAGAGCTTGGTAACTTCTAAGAATGAACCAAATTATTTTGGACCAGTCTCTATATCATTGCTTCCTTCCATGAACTACCAGTACACTTTTGTTTCAAACAAATCTCATAACAGCTATTCGGATGGAAGTGTTGTTCCCCCAAGTTCTTTAAAAATAGGGAGATTTTGCAGGGTTTTCTCAAAAGCAATACAATTCCGTGAGTTACAACTGAGATATTCAAGCCATTGTGGTTCTGCAAACAACTGCTCTCCTCTTGCTGTGTTTGATTTGCCTCGCGTCATGTCGTTGAATAATATTGAGTGTTTGGAGGATAGAAAAACGTTGCGAGTTCTGGTAGATTTTGCAGCCAGTAGCATCAGTTACCAGAGGCCTTTCAATCCAACTACAACATTGGTTGCCGAAGGTTCATGGGATGCAAGGAAAAATCAGCTAAATGTTGTTGCTTGTCGGTTCGTGCATGCAAGGAATTCTTTTAGTCCTAGTCATGTGGGTGATTGTTCAACACGATTGACAATGGGATTTCCTTCAATATGGACAATTGGAAATTCTAGCTGCATTGTGGGGAAAATTTGGAGCAACAAATCCGTGACAGAGGCAGGTTACTTCGAAAACATCACATTTGACAGTCCTCAAAGTGATTCAGCTGTATTTCCAGGCCAGAGGTATGAGTATACCAAAATAGACAAAGCAACAAAGTTGTGCCCAAGAAAGAAGACTGATGCTGATGACAAGAACAACATTTATCCAAATCCTTTTTCTTATTACATGGGATTGGATATTACAGCCAACAGTTCTATAGGAGAAGTTGGATGGGGCTATTCTATCCCTCTCTCTGTTGATAACAAATTCTTCCAACCGTATTGGTACTCTCTGGAAGATGGAACTGAAGAATACCTTGGAGCTCCTGTGAGTGCTCCTGTGAGCCATAGAAACAACAATAGCAACAGGTACAACATCAGCTACAGAATAAGCATTAAAGCAGTAGATGGGGATAGGCTTCCCAGTGGAGTGTCCCAACTTAATTCATTATATGAGATGGAGATTTTTGCTGAAGGGCTATATGATGGCGGAGATGGAAGCCTGTGTATGGTAGGTTGCCGATACTTGAGCTCAAACAGTCACCAACAGCCAACATATGATTCTGTAGACTGTGAGATTGTTGTTAAGGTTCAGTTTCCTCCAACAAATCCAAACAGTACCTATTTGGGTTTTATCAAGGGAAGCATCGAAAGCACAAGAGAAATGTCTGATCCTCTTCATTTTGAGGAATTGGATCTGACCTCAGCTGCTGGTAACGTGGTCGAAGCAAGCCAATCCATTTGGAGAATGGATGTGGAGATCATATTGGTTGTTATATCCAACACACTAGCTTGTGTTTTTGGGGCATTACAACTCTTTCATGTGAAACGGAATCCGGATGTGCTCCCATCCATTTCTATCTTGATGCTGTATATTCTTGCTCTTGGCTACATGATACCTCTTATGCTAAACTTTGATGCCTTGTTCACACAACATGGTAGCCAAACTGTGTTCCTTGGAAGTGGATGGCTTGAACTCAATGAAGTAATTGTAAGGGTAGTAACAATGGTAGGTTTCTTGCTGCAAATTCGTCTTCTGCAGATAACAATGTCAGCAAAATCAGCAAATGGAAACCAAAAGGAGCTCTGGGCAGTGGAGAAGCTGGCTCTCTTTGTGGCTTTACCAGTATATGTTGGAGGGTCCTTGGTTGCTTTGCTTCTGATGAACTGGAGGAGGAACGATATTGCTGTGACGGTTCCGAGTGGTCAAAAGCATCCCATTTTGGGTACTATTTTGAAATCTTGTGCTGGTTTGTTCTTGGATGCATTTTTGTTCCCTCAAATTCTTCTCAACATGTTCTCCAAATCAACGGAAAAGGCATTGTCAGTTTCGTTCTTCGTTGGAACAACTCTTGTTCGTGTGCTGCCACATGCATATGATCTTTACAGGGCTCATACCGCTGGTCACCAATTGAAAGATTCATACATTTATGCAAGCCCTGCAGCAGACTTTTACTCTACTGCTTGGAATGTCATTATTCCTTTTGGAGGTCTACTTTTTGCTGTGATAATCTACTTGCAGCAGCGCTTTGGGGGTTGCTGCATTCTTCCTCAGAAGTTCATAGAGTTGGTTGAATATGAGAAGGTGCCTGTCGTTACTGAAGCGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
DUF2921

Protein Analysis

894

Amino Acids

99.14

Weight (kDa)

6.51

Isoelectric Point (pI)

44.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2921_N PF25333 38 - 182 8.6e-17 DUF2921 lipocalin-like domain
DUF2921_N PF25333 203 - 371 3.7e-26 DUF2921 lipocalin-like domain
DUF2921_N PF25333 397 - 589 6e-32 DUF2921 lipocalin-like domain
DUF2921 PF11145 601 - 878 8.7e-61 Transmembrane E3 ligase/DUF2921 transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21700
fragaria_vesca FvH4_1g10360 FvH4_1g10760 FvH4_2g30370 FvH4_3g09240 FvH4_3g20050 FvH4_3g20280 FvH4_3g20290 FvH4_3g20291 FvH4_3g20340 FvH4_3g20350 FvH4_3g20351 FvH4_3g20380 FvH4_3g20390 FvH4_5g13190 FvH4_6g20070 FvH4_6g42890
malus_domestica MD03G1227000.v1.1 MD10G1294900.v1.1 MD11G1246300.v1.1 MD11G1246400.v1.1 MD11G1246500.v1.1 MD11G1246600.v1.1
prunus_persica Prupe.4G047300_v2.0.a1 Prupe.4G181700_v2.0.a1 Prupe.4G181900_v2.0.a1 Prupe.4G182000_v2.0.a1 Prupe.4G182200_v2.0.a1
pyrus_communis pycom03g17470 pycom10g24670 pycom11g21640
rosa_chinensis RchiOBHm_Chr5g0008381 RchiOBHm_Chr5g0008391 RchiOBHm_Chr5g0033881 RchiOBHm_Chr5g0034171 RchiOBHm_Chr5g0034181 RchiOBHm_Chr5g0034191 RchiOBHm_Chr5g0034201 RchiOBHm_Chr5g0034211 RchiOBHm_Chr5g0034221 RchiOBHm_Chr5g0034231 RchiOBHm_Chr5g0034271 RchiOBHm_Chr5g0034281
rosa_laevigata RLG00000031566 RLG00000031567 RLG00000033506 RLG00000033529 RLG00000033530 RLG00000033531
rosa_multiflora Rmu_co8445509.1_g000001 Rmu_co8470615.1_g000001 Rmu_sc0008805.1_g000003 Rmu_sc0008805.1_g000004 Rmu_ssc0000128.1_g000029 Rmu_ssc0000128.1_g000031 Rmu_ssc0000128.1_g000032 Rmu_ssc0000388.1_g000062 Rmu_ssc0000388.1_g000063
rosa_roxburghii Rroxscaffold_1G00046010 Rroxscaffold_1G00046020 Rroxscaffold_1G00046030 Rroxscaffold_1G00046040 Rroxscaffold_1G00046060 Rroxscaffold_1G00046070 Rroxscaffold_1G00046080 Rroxscaffold_1G00046090
rosa_rugosa Rorug04G0435500 Rorug05G0142900 Rorug05G0144600 Rorug05G0144600 Rorug05G0144700 Rorug05G0144800
rosa_samantha Rh5AG233800 Rh5AG235900 Rh5AG236000 Rh5AG236100 Rh5AG236200 Rh5AG236300 Rh5AG236500 Rh5BG061200 Rh5BG061300 Rh5BG234300 Rh5BG236600 Rh5BG236800 Rh5CG071200 Rh5DG060000 Rh5DG060100 Rh5DG241300 Rh5DG243700 Rh5DG244000 Rh5DG244100
rosa_wichuraiana Rw3G018950 Rw5G005680 Rw5G021330 Rw5G021340 Rw5G021350 Rw5G021550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1082
AccB1I GGYRCC 1 cut(s) 2659
AccI GTMKAC 3 cut(s) 1166, 1641, 2566
AccII CGCG 1 cut(s) 771
AccIII TCCGGA 2 cut(s) 177, 1909
AciI CCGC 5 cut(s) 106, 172, 194, 1563, 2472
AclI AACGTT 1 cut(s) 815
AclWI GGATC 3 cut(s) 391, 1739, 1776
AcsI RAATTY 6 cut(s) 961, 1039, 1062, 1340, 2100, 2355
AcuI CTGAAG 2 cut(s) 1401, 1566
AfaI GTAC 6 cut(s) 250, 578, 1361, 1445, 1693, 2302
AfeI AGCGCT 1 cut(s) 2600
AfiI CCNNNNNNNGG 3 cut(s) 1582, 2297, 2561
AflIII ACRYGT 1 cut(s) 2367
AgeI ACCGGT 1 cut(s) 113
AhlI ACTAGT 1 cut(s) 473
AjnI CCWGG 1 cut(s) 1147
AjuI GAANNNNNNNTTGG 8 cut(s) 213, 245, 1234, 1266, 1374, 1406, 1618, 1650
AloI GAACNNNNNNTCC 2 cut(s) 612, 644
Alw21I GWGCWC 5 cut(s) 1399, 1411, 1607, 1920, 2160
Alw26I GTCTC 1 cut(s) 547
AlwI GGATC 3 cut(s) 391, 1739, 1776
AlwNI CAGNNNCTG 1 cut(s) 1784
Aor13HI TCCGGA 2 cut(s) 177, 1909
Aor51HI AGCGCT 1 cut(s) 2600
AoxI GGCC 2 cut(s) 863, 1150
ApoI RAATTY 6 cut(s) 961, 1039, 1062, 1340, 2100, 2355
AsiGI ACCGGT 1 cut(s) 113
AspLEI GCGC 1 cut(s) 2601
AspS9I GGNCC 4 cut(s) 217, 403, 536, 2214
AsuHPI GGTGA 5 cut(s) 95, 146, 998, 1607, 2471
AsuII TTCGAA 1 cut(s) 1101
AvaII GGWCC 4 cut(s) 217, 403, 536, 2214
BaeGI GKGCMC 1 cut(s) 1199
BanI GGYRCC 1 cut(s) 2659
BanII GRGCYC 4 cut(s) 1399, 1607, 2160, 2467
BbsI GAAGAC 3 cut(s) 221, 1214, 2099
Bbv12I GWGCWC 5 cut(s) 1399, 1411, 1607, 1920, 2160
BbvCI CCTCAGC 1 cut(s) 1777
BciT130I CCWGG 1 cut(s) 1149
BclI TGATCA 1 cut(s) 31
BcoDI GTCTC 1 cut(s) 547
BcuI ACTAGT 1 cut(s) 473
BfaI CTAG 5 cut(s) 240, 474, 975, 1044, 1862
BfmI CTRYAG 6 cut(s) 124, 1290, 1456, 1638, 2111, 2514
BfoI RGCGCY 1 cut(s) 2602
BfuAI ACCTGC 1 cut(s) 1082
Bme1390I CCNGG 1 cut(s) 1149
Bme18I GGWCC 4 cut(s) 217, 403, 536, 2214
BmgT120I GGNCC 4 cut(s) 217, 403, 536, 2214
BmiI GGNNCC 3 cut(s) 2215, 2271, 2661
BmrFI CCNGG 1 cut(s) 1149
BmrI ACTGGG 1 cut(s) 1491
BmsI GCATC 8 cut(s) 858, 898, 1204, 1728, 1929, 1981, 2296, 2326
BmuI ACTGGG 1 cut(s) 1491
BpiI GAAGAC 3 cut(s) 221, 1214, 2099
BpmI CTGGAG 1 cut(s) 2263
Bpu10I CCTNAGC 1 cut(s) 1777
Bpu14I TTCGAA 1 cut(s) 1101
BpuEI CTTGAG 3 cut(s) 319, 437, 1621
BsaJI CCNNGG 6 cut(s) 220, 399, 406, 1390, 2029, 2217
BsaWI WCCGGW 3 cut(s) 113, 177, 1909
BsaXI ACNNNNNCTCC 4 cut(s) 1386, 1416, 2357, 2387
Bsc4I CCNNNNNNNGG 3 cut(s) 1582, 2297, 2561
Bse118I RCCGGY 1 cut(s) 113
Bse1I ACTGG 6 cut(s) 539, 574, 843, 1497, 2198, 2246
Bse3DI GCAATG 1 cut(s) 551
BseAI TCCGGA 2 cut(s) 177, 1909
BseBI CCWGG 1 cut(s) 1149
BseDI CCNNGG 6 cut(s) 220, 399, 406, 1390, 2029, 2217
BseLI CCNNNNNNNGG 3 cut(s) 1582, 2297, 2561
BseMI GCAATG 1 cut(s) 551
BseMII CTCAG 3 cut(s) 695, 1791, 2639
BseNI ACTGG 6 cut(s) 539, 574, 843, 1497, 2198, 2246
BseRI GAGGAG 4 cut(s) 42, 45, 735, 2260
BseSI GKGCMC 1 cut(s) 1199
Bsh1236I CGCG 1 cut(s) 771
BshFI GGCC 2 cut(s) 865, 1152
BshNI GGYRCC 1 cut(s) 2659
BshTI ACCGGT 1 cut(s) 113
BsiHKAI GWGCWC 5 cut(s) 1399, 1411, 1607, 1920, 2160
BsiSI CCGG 4 cut(s) 84, 114, 178, 1910
BslFI GGGAC 2 cut(s) 306, 1492
BslI CCNNNNNNNGG 3 cut(s) 1582, 2297, 2561
BsmAI GTCTC 1 cut(s) 547
BsmFI GGGAC 2 cut(s) 306, 1492
BsmI GAATGC 1 cut(s) 2616
BsnI GGCC 2 cut(s) 865, 1152
Bsp119I TTCGAA 1 cut(s) 1101
Bsp1286I GDGCHC 7 cut(s) 1199, 1399, 1411, 1607, 1920, 2160, 2467
Bsp13I TCCGGA 2 cut(s) 177, 1909
Bsp143I GATC 6 cut(s) 31, 383, 1744, 1768, 1833, 2452
BspACI CCGC 5 cut(s) 106, 172, 194, 1563, 2472
BspANI GGCC 2 cut(s) 865, 1152
BspCNI CTCAG 3 cut(s) 696, 1790, 2638
BspEI TCCGGA 2 cut(s) 177, 1909
BspFNI CGCG 1 cut(s) 771
BspLI GGNNCC 3 cut(s) 2215, 2271, 2661
BspMAI CTGCAG 2 cut(s) 2115, 2518
BspMI ACCTGC 1 cut(s) 1082
BspPI GGATC 3 cut(s) 391, 1739, 1776
BspT104I TTCGAA 1 cut(s) 1101
BspT107I GGYRCC 1 cut(s) 2659
BsrDI GCAATG 1 cut(s) 551
BsrFI RCCGGY 1 cut(s) 113
BsrI ACTGG 6 cut(s) 539, 574, 843, 1497, 2198, 2246
BssAI RCCGGY 1 cut(s) 113
BssECI CCNNGG 6 cut(s) 220, 399, 406, 1390, 2029, 2217
BssMI GATC 6 cut(s) 31, 383, 1744, 1768, 1833, 2452
BssNAI GTATAC 1 cut(s) 1167
BssT1I CCWWGG 5 cut(s) 220, 406, 1390, 2029, 2217
Bst1107I GTATAC 1 cut(s) 1167
Bst2UI CCWGG 1 cut(s) 1149
Bst6I CTCTTC 1 cut(s) 1755
BstAPI GCANNNNNTGC 1 cut(s) 2513
BstBI TTCGAA 1 cut(s) 1101
BstC8I GCNNGC 5 cut(s) 284, 954, 1804, 2178, 2509
BstDEI CTNAG 6 cut(s) 149, 493, 513, 704, 1777, 2625
BstEII GGTNACC 1 cut(s) 2477
BstENI CCTNNNNNAGG 1 cut(s) 2559
BstFNI CGCG 1 cut(s) 771
BstH2I RGCGCY 1 cut(s) 2602
BstHHI GCGC 1 cut(s) 2601
BstKTI GATC 6 cut(s) 34, 386, 1747, 1771, 1836, 2455
BstMAI GTCTC 1 cut(s) 547
BstMBI GATC 6 cut(s) 31, 383, 1744, 1768, 1833, 2452
BstMWI GCNNNNNNNGC 6 cut(s) 47, 498, 1725, 2135, 2471, 2513
BstNI CCWGG 1 cut(s) 1149
BstNSI RCATGY 3 cut(s) 956, 2371, 2447
BstPI GGTNACC 1 cut(s) 2477
BstSCI CCNGG 1 cut(s) 1147
BstSFI CTRYAG 6 cut(s) 124, 1290, 1456, 1638, 2111, 2514
BstSLI GKGCMC 1 cut(s) 1199
BstUI CGCG 1 cut(s) 771
BstV2I GAAGAC 3 cut(s) 221, 1214, 2099
BstX2I RGATCY 1 cut(s) 1768
BstYI RGATCY 1 cut(s) 1768
BstZ17I GTATAC 1 cut(s) 1167
BsuRI GGCC 2 cut(s) 865, 1152
BtsI GCAGTG 1 cut(s) 2172
BtsIMutI CAGTG 3 cut(s) 133, 1504, 2172
BveI ACCTGC 1 cut(s) 1082
Cac8I GCNNGC 5 cut(s) 284, 954, 1804, 2178, 2509
CaiI CAGNNNCTG 1 cut(s) 1784
CfoI GCGC 1 cut(s) 2601
Cfr10I RCCGGY 1 cut(s) 113
Cfr13I GGNCC 4 cut(s) 217, 403, 536, 2214
CseI GACGC 3 cut(s) 308, 760, 2667
Csp6I GTAC 6 cut(s) 249, 577, 1360, 1444, 1692, 2301
CspAI ACCGGT 1 cut(s) 113
CviQI GTAC 6 cut(s) 249, 577, 1360, 1444, 1692, 2301
DdeI CTNAG 6 cut(s) 149, 493, 513, 704, 1777, 2625
DpnI GATC 6 cut(s) 33, 385, 1746, 1770, 1835, 2454
DpnII GATC 6 cut(s) 31, 383, 1744, 1768, 1833, 2452
DraI TTTAAA 1 cut(s) 645
Eam1104I CTCTTC 1 cut(s) 1755
EarI CTCTTC 1 cut(s) 1755
EciI GGCGGA 1 cut(s) 1578
Ecl136II GAGCTC 3 cut(s) 1397, 1605, 2158
Eco130I CCWWGG 5 cut(s) 220, 406, 1390, 2029, 2217
Eco147I AGGCCT 1 cut(s) 865
Eco24I GRGCYC 4 cut(s) 1399, 1607, 2160, 2467
Eco47I GGWCC 4 cut(s) 217, 403, 536, 2214
Eco47III AGCGCT 1 cut(s) 2600
Eco53kI GAGCTC 3 cut(s) 1397, 1605, 2158
Eco57I CTGAAG 2 cut(s) 1401, 1566
Eco91I GGTNACC 1 cut(s) 2477
EcoICRI GAGCTC 3 cut(s) 1397, 1605, 2158
EcoNI CCTNNNNNAGG 1 cut(s) 2559
EcoO109I RGGNCCY 1 cut(s) 2214
EcoO65I GGTNACC 1 cut(s) 2477
EcoRI GAATTC 1 cut(s) 961
EcoRII CCWGG 1 cut(s) 1147
EcoT14I CCWWGG 5 cut(s) 220, 406, 1390, 2029, 2217
EcoT22I ATGCAT 2 cut(s) 2341, 2449
EcoT38I GRGCYC 4 cut(s) 1399, 1607, 2160, 2467
ErhI CCWWGG 5 cut(s) 220, 406, 1390, 2029, 2217
FalI AAGNNNNNCTT 6 cut(s) 213, 245, 949, 981, 1374, 1406
FaqI GGGAC 2 cut(s) 306, 1492
FauNDI CATATG 3 cut(s) 310, 1630, 2449
FbaI TGATCA 1 cut(s) 31
FblI GTMKAC 3 cut(s) 1166, 1641, 2566
FriOI GRGCYC 4 cut(s) 1399, 1607, 2160, 2467
FspBI CTAG 5 cut(s) 240, 474, 975, 1044, 1862
GlaI GCGC 1 cut(s) 2600
GsuI CTGGAG 1 cut(s) 2263
HaeII RGCGCY 1 cut(s) 2602
HaeIII GGCC 2 cut(s) 865, 1152
HapII CCGG 4 cut(s) 84, 114, 178, 1910
HgaI GACGC 3 cut(s) 308, 760, 2667
HhaI GCGC 1 cut(s) 2601
Hin6I GCGC 1 cut(s) 2599
HinP1I GCGC 1 cut(s) 2599
HindIII AAGCTT 1 cut(s) 328
HinfI GANTC 5 cut(s) 356, 1133, 1634, 1906, 2492
HpaII CCGG 4 cut(s) 84, 114, 178, 1910
HphI GGTGA 5 cut(s) 95, 146, 998, 1607, 2471
Hpy166II GTNNAC 6 cut(s) 103, 579, 1167, 1642, 2001, 2567
Hpy188I TCNGA 9 cut(s) 156, 355, 403, 616, 1744, 1773, 2235, 2274, 2628
Hpy188III TCNNGA 5 cut(s) 178, 454, 1367, 1910, 1936
Hpy8I GTNNAC 6 cut(s) 103, 579, 1167, 1642, 2001, 2567
HpyAV CCTTC 5 cut(s) 570, 890, 1029, 1541, 2650
HpyCH4IV ACGT 2 cut(s) 815, 1791
HpyF10VI GCNNNNNNNGC 6 cut(s) 47, 498, 1725, 2135, 2471, 2513
HpyF3I CTNAG 6 cut(s) 149, 493, 513, 704, 1777, 2625
HpySE526I ACGT 2 cut(s) 815, 1791
HspAI GCGC 1 cut(s) 2599
Kpn2I TCCGGA 2 cut(s) 177, 1909
Ksp22I TGATCA 1 cut(s) 31
Kzo9I GATC 6 cut(s) 31, 383, 1744, 1768, 1833, 2452
LmnI GCTCC 9 cut(s) 55, 279, 367, 1068, 1394, 1402, 1414, 1923, 2155
LweI GCATC 8 cut(s) 858, 898, 1204, 1728, 1929, 1981, 2296, 2326
MaeI CTAG 5 cut(s) 240, 474, 975, 1044, 1862
MaeII ACGT 2 cut(s) 815, 1791
MalI GATC 6 cut(s) 33, 385, 1746, 1770, 1835, 2454
MboI GATC 6 cut(s) 31, 383, 1744, 1768, 1833, 2452
MfeI CAATTG 4 cut(s) 45, 162, 1032, 2483
MflI RGATCY 1 cut(s) 1768
MhlI GDGCHC 7 cut(s) 1199, 1399, 1411, 1607, 1920, 2160, 2467
MmeI TCCRAC 8 cut(s) 361, 899, 1282, 1372, 1700, 1878, 2188, 2395
Mph1103I ATGCAT 2 cut(s) 2341, 2449
MroI TCCGGA 2 cut(s) 177, 1909
MseI TTAA 8 cut(s) 90, 438, 479, 644, 1470, 1515, 1659, 2683
MslI CAYNNNNRTG 2 cut(s) 984, 2448
MspA1I CMGCKG 3 cut(s) 1139, 1781, 2474
MspI CCGG 4 cut(s) 84, 114, 178, 1910
MspR9I CCNGG 1 cut(s) 1149
MunI CAATTG 4 cut(s) 45, 162, 1032, 2483
Mva1269I GAATGC 1 cut(s) 2616
MvaI CCWGG 1 cut(s) 1149
MvnI CGCG 1 cut(s) 771
MwoI GCNNNNNNNGC 6 cut(s) 47, 498, 1725, 2135, 2471, 2513
NdeI CATATG 3 cut(s) 310, 1630, 2449
NdeII GATC 6 cut(s) 31, 383, 1744, 1768, 1833, 2452
NlaIV GGNNCC 3 cut(s) 2215, 2271, 2661
NmuCI GTSAC 5 cut(s) 242, 1081, 1613, 2263, 2477
NsiI ATGCAT 2 cut(s) 2341, 2449
NspI RCATGY 3 cut(s) 956, 2371, 2447
NspV TTCGAA 1 cut(s) 1101
PaeI GCATGC 1 cut(s) 956
PceI AGGCCT 1 cut(s) 865
PciI ACATGT 1 cut(s) 2367
PctI GAATGC 1 cut(s) 2616
PfeI GAWTC 5 cut(s) 356, 1133, 1634, 1906, 2492
PflFI GACNNNGTC 1 cut(s) 318
PinAI ACCGGT 1 cut(s) 113
PpuMI RGGWCCY 1 cut(s) 2214
PscI ACATGT 1 cut(s) 2367
Psp124BI GAGCTC 3 cut(s) 1399, 1607, 2160
Psp1406I AACGTT 1 cut(s) 815
Psp5II RGGWCCY 1 cut(s) 2214
Psp6I CCWGG 1 cut(s) 1147
PspEI GGTNACC 1 cut(s) 2477
PspGI CCWGG 1 cut(s) 1147
PspN4I GGNNCC 3 cut(s) 2215, 2271, 2661
PspPI GGNCC 4 cut(s) 217, 403, 536, 2214
PspPPI RGGWCCY 1 cut(s) 2214
PsrI GAACNNNNNNTAC 2 cut(s) 560, 592
PstI CTGCAG 2 cut(s) 2115, 2518
PstNI CAGNNNCTG 1 cut(s) 1784
PsuI RGATCY 1 cut(s) 1768
PsyI GACNNNGTC 1 cut(s) 318
PvuII CAGCTG 2 cut(s) 1139, 1781
RsaI GTAC 6 cut(s) 250, 578, 1361, 1445, 1693, 2302
RsaNI GTAC 6 cut(s) 249, 577, 1360, 1444, 1692, 2301
RseI CAYNNNNRTG 2 cut(s) 984, 2448
SacI GAGCTC 3 cut(s) 1399, 1607, 2160
SaqAI TTAA 8 cut(s) 90, 438, 479, 644, 1470, 1515, 1659, 2683
Sau3AI GATC 6 cut(s) 31, 383, 1744, 1768, 1833, 2452
Sau96I GGNCC 4 cut(s) 217, 403, 536, 2214
ScrFI CCNGG 1 cut(s) 1149
SduI GDGCHC 7 cut(s) 1199, 1399, 1411, 1607, 1920, 2160, 2467
SfaNI GCATC 8 cut(s) 858, 898, 1204, 1728, 1929, 1981, 2296, 2326
SfcI CTRYAG 6 cut(s) 124, 1290, 1456, 1638, 2111, 2514
SfuI TTCGAA 1 cut(s) 1101
SinI GGWCC 4 cut(s) 217, 403, 536, 2214
SmiMI CAYNNNNRTG 2 cut(s) 984, 2448
SmlI CTYRAG 4 cut(s) 15, 298, 452, 1600
SmoI CTYRAG 4 cut(s) 15, 298, 452, 1600
SpeI ACTAGT 1 cut(s) 473
SphI GCATGC 1 cut(s) 956
SseBI AGGCCT 1 cut(s) 865
SsiI CCGC 5 cut(s) 106, 172, 194, 1563, 2472
SspI AATATT 1 cut(s) 790
SspMI CTAG 5 cut(s) 240, 474, 975, 1044, 1862
SstI GAGCTC 3 cut(s) 1399, 1607, 2160
StuI AGGCCT 1 cut(s) 865
StyD4I CCNGG 1 cut(s) 1147
StyI CCWWGG 5 cut(s) 220, 406, 1390, 2029, 2217
TaiI ACGT 2 cut(s) 818, 1794
TaqI TCGA 4 cut(s) 232, 1101, 1722, 1797
TatI WGTACW 2 cut(s) 248, 576
TfiI GAWTC 5 cut(s) 356, 1133, 1634, 1906, 2492
Tru1I TTAA 8 cut(s) 90, 438, 479, 644, 1470, 1515, 1659, 2683
Tru9I TTAA 8 cut(s) 90, 438, 479, 644, 1470, 1515, 1659, 2683
TscAI CASTG 3 cut(s) 133, 1504, 2172
TseFI GTSAC 5 cut(s) 242, 1081, 1613, 2263, 2477
Tsp45I GTSAC 5 cut(s) 242, 1081, 1613, 2263, 2477
TspGWI ACGGA 4 cut(s) 680, 1069, 1918, 2399
TspRI CASTG 3 cut(s) 133, 1504, 2172
Tth111I GACNNNGTC 1 cut(s) 318
VpaK11BI GGWCC 4 cut(s) 217, 403, 536, 2214
XagI CCTNNNNNAGG 1 cut(s) 2559
XapI RAATTY 6 cut(s) 961, 1039, 1062, 1340, 2100, 2355
XceI RCATGY 3 cut(s) 956, 2371, 2447
XcmI CCANNNNNNNNNTGG 1 cut(s) 1819
XmiI GTMKAC 3 cut(s) 1166, 1641, 2566
XspI CTAG 5 cut(s) 240, 474, 975, 1044, 1862
Zsp2I ATGCAT 2 cut(s) 2341, 2449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.