FvH4_3g20340

Protein of unknown function (DUF2921)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000420
Physical Location & Seq
Reverse (-)
13399015 .. 13402105
3091 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g20340.t1

Sequence Viewer

Length: 2391 bp
ATGCAGGTAAAAAATTCAGTTGGCTTCCAATGCTTGGATATTAGAGAAACTCATGTACAAGGTTTGTTCAAGCTTGAGGGTACTCTTGATTTTCAAGTAACCAATAGGTTTCAAGCCGACAGCATGCCGCCTATAGTAACATCAGGTTCGGTTTCCTTTAGACTAAATGGATTCTGGTCTGAACCTTCTGGGAATCTTTGCATGGTTGGATCATCAACTTCCATGTACTCGAAACTAGGTAATCCACTTATTGTTCTTAAGCTGTATAATCTTATGAATTCCACTAGTATTACAAGTTTGATTAGCGGAACCTTTGGGAATGTCATGAATACTAAGAAAGATCTCAAGTATTTGGGACCAATCTCTGTTTTGATGTTTCCTCGCATAAACTACGCGTACACTTTGGTTTCAAGTACTACTGATAACAGTTCCTCTAGTCGAAGTGAAGACACCCCAAGCTCTATACAAGCACAGAGATACCCCAAGCTGTTTTGTTCACTACTTTCATCAGTAGTAATAAATCATGACTTCCCTCTCAAATTTTCAGGCCACTACTGCATTTTGGCAAAGAATTGCACTCCAATTTCTACGTCTGAATCACCTCGTGCTGTGTTGTTTAAGGACATATCGTGCTCGGAGGATAATGGAAGGGTGAGAGTTCTGGTAGAATTTACAGACGGTAGGCTAAACCAGTACCAGTGTCCAACGCTCTTCAATCCTAATACAACTTTAGTTGGTGAAGGCTCATGGGATGCAAAGAAGAATCAGCTTTATGTTGTTGCTTGTCGATTCTTGAATACGACACATTCTTTGAATAGTACTAATGTGGGTGATTGTTCAACAAGATTAAGCTTGAGAATTCATGCAGAAGGAACAATGGGAAGAACCAGCAACATTGTGGGTCAGATTTGGAGCACTAAAACTGTGACAGAGTTGGGCTACTTTGAGAAGATCACATTTGCAAGTCGGGAACGGTGGCTTCCGGTCAAGCAGCTGCATGGATTTGGCAGCGGTAGTAATCCGTGGCAGATGGATTGGTGGATGTCAGAAAGGATTTTAGGGTTGAGCTTGAGAATTCCTGCAAAAGGGACGATCGGAAGTACTAGCAGCATTGTGGGGGAGATTTGGAGCACCAAAACTGTGACAGAGTTGGGCTACTTCGATAAGATGACAATTGCAAGTCGGGACTGTGGTTGTCGAAAGGGTCTCCTTCCAGGCCAGACATATGAGTTTACTAAAACGGAGAAAGTAACAAAGTTGTGCTCAAGAAAGAAGTCCGCAAACAACAAGGCCGGCATATACCCAAATCCATTAACGCATGCCAGGAGTTTCATTATGTATGCCCAAAACCCTAAACCCAAGGACAAGTTGGGTTTGGCAATTGTGTTCCTCTCTCTGTTGACAATACAGGCAAAAAATGATTCGACAGATTGTGAGATTCTTGTCAATTTTCGGTTCCCAGCAACAAATGAAAGGGATTTGGGTCATATCAAAGGAAGCATTGAGAGCACACGAAAGAATTCTGACCCTCTTCATTTTGAATGCTTGGATTTGTCTACAGCTGCCAAGATTGATGAAGAACAGCGATCCATTTGGAGGATGGATGGAAAGTTAACTGTGGTTCTTATATCCACCAGTCTTGCATGTGTTTTTGCTGCATTACAACTCTTCCATGTGAAAAGAAATCCTGAAGTTATTCCCCCTATTTCCATCTTAATGCTTCTAATACTTACCCTTGGCTACATGTTACCTCTGGGGATAAACTTTGAAGCCATGTTCATACATAATTTCAACCGCCAGAATGTGCACCTTGGAGGGGGTGGATGGCTTAAATTTAATGAGATAATCATAAGGGTGACAACATTGGTAGCTTTCTTGATATTATTCCACCTTCTGAAGCTAACTTGGTCAGCAAAATCAGGAAATGGAACCCGAAAGGATATCTGGATCATGGAAAAGAAGGCTCTCTTTGTAGCTTTACGAGTGTATGCTGTTGGGGCTGTAGCTGCCTTGTTTCTGCGTAGACCGATCCTGAGGAAGAGGGACTACGGCATTGAGCTGCCTCATGAATCTAGAGAATACAGTTCGCTGGATGCCTTGACATGTTATGTTTGGTTCTACATTGGGACAACCGTAGCTCGAGTTCTGCCACATGCATATGATCTTTACAGGGCTCACAGCTCTGCTACTGACAATGATTACTCATACAATTATCCAGGTCCTCATGAAGATTTATACCCCATAGCTTGGAATACCATCATTCCTTTAGGAGGTGTACTATTTGCCAGTGTCATTTACTTGCAGCAGCGGTTTAGGGGTGGTGGCGTTACTCCTGCAAAGTTGCAAGAGGTGAGTACAGATGAGAAGCTCCCTGCCGTCCATGAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
DUF2921

Protein Analysis

797

Amino Acids

89.09

Weight (kDa)

9.14

Isoelectric Point (pI)

35.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2921_N PF25333 13 - 122 4.3e-10 DUF2921 lipocalin-like domain
DUF2921_N PF25333 153 - 319 1.4e-21 DUF2921 lipocalin-like domain
DUF2921_N PF25333 472 - 514 9.2e-11 DUF2921 lipocalin-like domain
DUF2921 PF11145 528 - 772 1.3e-35 Transmembrane E3 ligase/DUF2921 transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21700
fragaria_vesca FvH4_1g10360 FvH4_1g10760 FvH4_2g30370 FvH4_3g09240 FvH4_3g20050 FvH4_3g20280 FvH4_3g20290 FvH4_3g20291 FvH4_3g20340 FvH4_3g20350 FvH4_3g20351 FvH4_3g20380 FvH4_3g20390 FvH4_5g13190 FvH4_6g20070 FvH4_6g42890
malus_domestica MD03G1227000.v1.1 MD10G1294900.v1.1 MD11G1246300.v1.1 MD11G1246400.v1.1 MD11G1246500.v1.1 MD11G1246600.v1.1
prunus_persica Prupe.4G047300_v2.0.a1 Prupe.4G181700_v2.0.a1 Prupe.4G181900_v2.0.a1 Prupe.4G182000_v2.0.a1 Prupe.4G182200_v2.0.a1
pyrus_communis pycom03g17470 pycom10g24670 pycom11g21640
rosa_chinensis RchiOBHm_Chr5g0008381 RchiOBHm_Chr5g0008391 RchiOBHm_Chr5g0033881 RchiOBHm_Chr5g0034171 RchiOBHm_Chr5g0034181 RchiOBHm_Chr5g0034191 RchiOBHm_Chr5g0034201 RchiOBHm_Chr5g0034211 RchiOBHm_Chr5g0034221 RchiOBHm_Chr5g0034231 RchiOBHm_Chr5g0034271 RchiOBHm_Chr5g0034281
rosa_laevigata RLG00000031566 RLG00000031567 RLG00000033506 RLG00000033529 RLG00000033530 RLG00000033531
rosa_multiflora Rmu_co8445509.1_g000001 Rmu_co8470615.1_g000001 Rmu_sc0008805.1_g000003 Rmu_sc0008805.1_g000004 Rmu_ssc0000128.1_g000029 Rmu_ssc0000128.1_g000031 Rmu_ssc0000128.1_g000032 Rmu_ssc0000388.1_g000062 Rmu_ssc0000388.1_g000063
rosa_roxburghii Rroxscaffold_1G00046010 Rroxscaffold_1G00046020 Rroxscaffold_1G00046030 Rroxscaffold_1G00046040 Rroxscaffold_1G00046060 Rroxscaffold_1G00046070 Rroxscaffold_1G00046080 Rroxscaffold_1G00046090
rosa_rugosa Rorug04G0435500 Rorug05G0142900 Rorug05G0144600 Rorug05G0144600 Rorug05G0144700 Rorug05G0144800
rosa_samantha Rh5AG233800 Rh5AG235900 Rh5AG236000 Rh5AG236100 Rh5AG236200 Rh5AG236300 Rh5AG236500 Rh5BG061200 Rh5BG061300 Rh5BG234300 Rh5BG236600 Rh5BG236800 Rh5CG071200 Rh5DG060000 Rh5DG060100 Rh5DG241300 Rh5DG243700 Rh5DG244000 Rh5DG244100
rosa_wichuraiana Rw3G018950 Rw5G005680 Rw5G021330 Rw5G021340 Rw5G021350 Rw5G021550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 34, 2247
AccI GTMKAC 2 cut(s) 1556, 2023
AccII CGCG 1 cut(s) 395
AciI CCGC 6 cut(s) 128, 306, 1011, 1278, 1795, 2308
AclWI GGATC 4 cut(s) 217, 1581, 1955, 2023
AcsI RAATTY 8 cut(s) 13, 277, 539, 668, 858, 1074, 1519, 1832
AcuI CTGAAG 2 cut(s) 1710, 1916
AdeI CACNNNGTG 1 cut(s) 605
AfiI CCNNNNNNNGG 6 cut(s) 34, 1085, 1596, 1816, 2247, 2270
AflII CTTAAG 1 cut(s) 257
AflIII ACRYGT 3 cut(s) 393, 1743, 2102
AhlI ACTAGT 1 cut(s) 284
AjnI CCWGG 3 cut(s) 1213, 1322, 2215
Alw21I GWGCWC 6 cut(s) 635, 917, 1133, 1265, 1511, 1809
Alw26I GTCTC 1 cut(s) 1211
Alw44I GTGCAC 1 cut(s) 1805
AlwI GGATC 4 cut(s) 217, 1581, 1955, 2023
Ama87I CYCGRG 1 cut(s) 2139
AoxI GGCC 3 cut(s) 547, 1216, 1290
ApaLI GTGCAC 1 cut(s) 1805
ApoI RAATTY 8 cut(s) 13, 277, 539, 668, 858, 1074, 1519, 1832
ArsI GACNNNNNNTTYG 2 cut(s) 793, 825
Asp700I GAANNNNTTC 2 cut(s) 1519, 1695
AspS9I GGNCC 2 cut(s) 356, 2219
AsuHPI GGTGA 6 cut(s) 591, 664, 749, 842, 1867, 2362
AvaI CYCGRG 1 cut(s) 2139
AvaII GGWCC 2 cut(s) 356, 2219
AxyI CCTNAGG 1 cut(s) 2033
BaeGI GKGCMC 1 cut(s) 1809
BanII GRGCYC 1 cut(s) 2176
BarI GAAGNNNNNNTAC 2 cut(s) 2030, 2062
BauI CACGAG 1 cut(s) 603
BbsI GAAGAC 1 cut(s) 453
Bbv12I GWGCWC 6 cut(s) 635, 917, 1133, 1265, 1511, 1809
BccI CCATC 6 cut(s) 1024, 1594, 1598, 1718, 1818, 2264
BceAI ACGGC 2 cut(s) 2065, 2360
BciT130I CCWGG 3 cut(s) 1215, 1324, 2217
BcoDI GTCTC 1 cut(s) 1211
BcuI ACTAGT 1 cut(s) 284
BfaI CTAG 5 cut(s) 236, 285, 435, 1104, 2073
BfmI CTRYAG 3 cut(s) 132, 1557, 2001
BfrI CTTAAG 1 cut(s) 257
BglII AGATCT 1 cut(s) 340
BmcAI AGTACT 3 cut(s) 415, 820, 1102
Bme1390I CCNGG 3 cut(s) 1215, 1324, 2217
Bme18I GGWCC 2 cut(s) 356, 2219
BmeT110I CYCGRG 1 cut(s) 2139
BmgT120I GGNCC 2 cut(s) 356, 2219
BmiI GGNNCC 4 cut(s) 310, 357, 1457, 1930
BmrFI CCNGG 3 cut(s) 1215, 1324, 2217
BmsI GCATC 2 cut(s) 742, 2083
BpiI GAAGAC 1 cut(s) 453
BpuEI CTTGAG 5 cut(s) 95, 329, 874, 1090, 1249
BsaI GGTCTC 1 cut(s) 1211
BsaJI CCNNGG 4 cut(s) 1022, 1359, 1735, 1810
BsaWI WCCGGW 1 cut(s) 982
Bsc4I CCNNNNNNNGG 6 cut(s) 34, 1085, 1596, 1816, 2247, 2270
Bse118I RCCGGY 1 cut(s) 1292
Bse1I ACTGG 4 cut(s) 691, 697, 1635, 2286
Bse21I CCTNAGG 1 cut(s) 2033
BseBI CCWGG 3 cut(s) 1215, 1324, 2217
BseDI CCNNGG 4 cut(s) 1022, 1359, 1735, 1810
BseGI GGATG 6 cut(s) 757, 1047, 1605, 1609, 1829, 2098
BseLI CCNNNNNNNGG 6 cut(s) 34, 1085, 1596, 1816, 2247, 2270
BseMII CTCAG 1 cut(s) 2024
BseNI ACTGG 4 cut(s) 691, 697, 1635, 2286
BseSI GKGCMC 1 cut(s) 1809
BseYI CCCAGC 1 cut(s) 1459
Bsh1236I CGCG 1 cut(s) 395
Bsh1285I CGRYCG 1 cut(s) 1095
BshFI GGCC 3 cut(s) 549, 1218, 1292
BsiEI CGRYCG 1 cut(s) 1095
BsiHKAI GWGCWC 6 cut(s) 635, 917, 1133, 1265, 1511, 1809
BsiHKCI CYCGRG 1 cut(s) 2139
BsiSI CCGG 2 cut(s) 983, 1293
BslFI GGGAC 5 cut(s) 369, 1102, 1199, 2057, 2140
BslI CCNNNNNNNGG 6 cut(s) 34, 1085, 1596, 1816, 2247, 2270
BsmAI GTCTC 1 cut(s) 1211
BsmFI GGGAC 5 cut(s) 369, 1102, 1199, 2057, 2140
BsmI GAATGC 1 cut(s) 1547
BsnI GGCC 3 cut(s) 549, 1218, 1292
Bso31I GGTCTC 1 cut(s) 1211
BsoBI CYCGRG 1 cut(s) 2139
Bsp1286I GDGCHC 7 cut(s) 635, 917, 1133, 1265, 1511, 1809, 2176
Bsp1407I TGTACA 1 cut(s) 55
Bsp143I GATC 8 cut(s) 209, 340, 951, 1092, 1586, 1947, 2028, 2161
BspACI CCGC 6 cut(s) 128, 306, 1011, 1278, 1795, 2308
BspANI GGCC 3 cut(s) 549, 1218, 1292
BspCNI CTCAG 1 cut(s) 2025
BspFNI CGCG 1 cut(s) 395
BspHI TCATGA 4 cut(s) 324, 523, 2065, 2224
BspLI GGNNCC 4 cut(s) 310, 357, 1457, 1930
BspPI GGATC 4 cut(s) 217, 1581, 1955, 2023
BspQI GCTCTTC 1 cut(s) 716
BspTI CTTAAG 1 cut(s) 257
BspTNI GGTCTC 1 cut(s) 1211
BsrFI RCCGGY 1 cut(s) 1292
BsrGI TGTACA 1 cut(s) 55
BsrI ACTGG 4 cut(s) 691, 697, 1635, 2286
BssAI RCCGGY 1 cut(s) 1292
BssECI CCNNGG 4 cut(s) 1022, 1359, 1735, 1810
BssMI GATC 8 cut(s) 209, 340, 951, 1092, 1586, 1947, 2028, 2161
BssSI CACGAG 1 cut(s) 603
BssT1I CCWWGG 3 cut(s) 1359, 1735, 1810
Bst2BI CACGAG 1 cut(s) 603
Bst2UI CCWGG 3 cut(s) 1215, 1324, 2217
Bst4CI ACNGT 9 cut(s) 428, 680, 925, 975, 1141, 1190, 1618, 2084, 2134
Bst6I CTCTTC 5 cut(s) 716, 1536, 1673, 2033, 2379
BstAFI CTTAAG 1 cut(s) 257
BstAUI TGTACA 1 cut(s) 55
BstC8I GCNNGC 3 cut(s) 125, 1294, 1320
BstDEI CTNAG 2 cut(s) 333, 2033
BstDSI CCRYGG 1 cut(s) 1022
BstENI CCTNNNNNAGG 2 cut(s) 1083, 2268
BstF5I GGATG 6 cut(s) 757, 1047, 1605, 1609, 1829, 2098
BstFNI CGCG 1 cut(s) 395
BstKTI GATC 8 cut(s) 212, 343, 954, 1095, 1589, 1950, 2031, 2164
BstMAI GTCTC 1 cut(s) 1211
BstMBI GATC 8 cut(s) 209, 340, 951, 1092, 1586, 1947, 2028, 2161
BstMCI CGRYCG 1 cut(s) 1095
BstMWI GCNNNNNNNGC 5 cut(s) 30, 555, 1506, 1997, 2006
BstNI CCWGG 3 cut(s) 1215, 1324, 2217
BstNSI RCATGY 6 cut(s) 127, 1322, 1647, 1747, 2106, 2156
BstSCI CCNGG 3 cut(s) 1213, 1322, 2215
BstSFI CTRYAG 3 cut(s) 132, 1557, 2001
BstSLI GKGCMC 1 cut(s) 1809
BstUI CGCG 1 cut(s) 395
BstV2I GAAGAC 1 cut(s) 453
BstX2I RGATCY 1 cut(s) 340
BstYI RGATCY 1 cut(s) 340
Bsu36I CCTNAGG 1 cut(s) 2033
BsuRI GGCC 3 cut(s) 549, 1218, 1292
BtgI CCRYGG 1 cut(s) 1022
BtsCI GGATG 6 cut(s) 757, 1047, 1605, 1609, 1829, 2098
BtsIMutI CAGTG 2 cut(s) 704, 2293
Cac8I GCNNGC 3 cut(s) 125, 1294, 1320
CciI TCATGA 4 cut(s) 324, 523, 2065, 2224
Cfr10I RCCGGY 1 cut(s) 1292
Cfr13I GGNCC 2 cut(s) 356, 2219
CspCI CAANNNNNGTGG 2 cut(s) 1621, 1656
DdeI CTNAG 2 cut(s) 333, 2033
DpnI GATC 8 cut(s) 211, 342, 953, 1094, 1588, 1949, 2030, 2163
DpnII GATC 8 cut(s) 209, 340, 951, 1092, 1586, 1947, 2028, 2161
DraIII CACNNNGTG 1 cut(s) 605
Eam1104I CTCTTC 5 cut(s) 716, 1536, 1673, 2033, 2379
EarI CTCTTC 5 cut(s) 716, 1536, 1673, 2033, 2379
Eco130I CCWWGG 3 cut(s) 1359, 1735, 1810
Eco24I GRGCYC 1 cut(s) 2176
Eco31I GGTCTC 1 cut(s) 1211
Eco32I GATATC 1 cut(s) 1942
Eco47I GGWCC 2 cut(s) 356, 2219
Eco57I CTGAAG 2 cut(s) 1710, 1916
Eco81I CCTNAGG 1 cut(s) 2033
Eco88I CYCGRG 1 cut(s) 2139
EcoNI CCTNNNNNAGG 2 cut(s) 1083, 2268
EcoO109I RGGNCCY 1 cut(s) 2219
EcoRI GAATTC 4 cut(s) 277, 858, 1074, 1519
EcoRII CCWGG 3 cut(s) 1213, 1322, 2215
EcoRV GATATC 1 cut(s) 1942
EcoT14I CCWWGG 3 cut(s) 1359, 1735, 1810
EcoT22I ATGCAT 1 cut(s) 2158
EcoT38I GRGCYC 1 cut(s) 2176
ErhI CCWWGG 3 cut(s) 1359, 1735, 1810
FalI AAGNNNNNCTT 2 cut(s) 1952, 1984
FaqI GGGAC 5 cut(s) 369, 1102, 1199, 2057, 2140
FauNDI CATATG 2 cut(s) 1225, 2158
FblI GTMKAC 2 cut(s) 1556, 2023
FokI GGATG 6 cut(s) 764, 1054, 1612, 1616, 1836, 2105
FriOI GRGCYC 1 cut(s) 2176
FspBI CTAG 5 cut(s) 236, 285, 435, 1104, 2073
GsaI CCCAGC 1 cut(s) 1463
HaeIII GGCC 3 cut(s) 549, 1218, 1292
HapII CCGG 2 cut(s) 983, 1293
HincII GTYRAC 2 cut(s) 1401, 1614
HindII GTYRAC 2 cut(s) 1401, 1614
HindIII AAGCTT 2 cut(s) 71, 850
HinfI GANTC 8 cut(s) 171, 193, 596, 763, 789, 1421, 1438, 2069
HpaI GTTAAC 1 cut(s) 1614
HpaII CCGG 2 cut(s) 983, 1293
HphI GGTGA 6 cut(s) 591, 664, 749, 842, 1867, 2362
Hpy166II GTNNAC 9 cut(s) 399, 497, 1233, 1401, 1557, 1614, 1807, 2024, 2276
Hpy188I TCNGA 8 cut(s) 181, 595, 637, 906, 1048, 1097, 1525, 1896
Hpy8I GTNNAC 9 cut(s) 399, 497, 1233, 1401, 1557, 1614, 1807, 2024, 2276
HpyAV CCTTC 7 cut(s) 195, 642, 734, 863, 1220, 1901, 1954
HpyCH4III ACNGT 9 cut(s) 428, 680, 925, 975, 1141, 1190, 1618, 2084, 2134
HpyCH4IV ACGT 1 cut(s) 590
HpyF10VI GCNNNNNNNGC 5 cut(s) 30, 555, 1506, 1997, 2006
HpyF3I CTNAG 2 cut(s) 333, 2033
HpySE526I ACGT 1 cut(s) 590
KroI GCCGGC 1 cut(s) 1292
KroNI GCCGGC 1 cut(s) 1294
KspAI GTTAAC 1 cut(s) 1614
Kzo9I GATC 8 cut(s) 209, 340, 951, 1092, 1586, 1947, 2028, 2161
LguI GCTCTTC 1 cut(s) 716
LmnI GCTCC 3 cut(s) 912, 1128, 2373
LweI GCATC 2 cut(s) 742, 2083
MaeI CTAG 5 cut(s) 236, 285, 435, 1104, 2073
MaeII ACGT 1 cut(s) 590
MaeIII GTNAC 8 cut(s) 97, 136, 925, 1141, 1249, 1746, 1855, 2326
MalI GATC 8 cut(s) 211, 342, 953, 1094, 1588, 1949, 2030, 2163
MboI GATC 8 cut(s) 209, 340, 951, 1092, 1586, 1947, 2028, 2161
MfeI CAATTG 2 cut(s) 1173, 1380
MflI RGATCY 1 cut(s) 340
MhlI GDGCHC 7 cut(s) 635, 917, 1133, 1265, 1511, 1809, 2176
MluI ACGCGT 1 cut(s) 393
MmeI TCCRAC 2 cut(s) 187, 728
Mph1103I ATGCAT 1 cut(s) 2158
MroNI GCCGGC 1 cut(s) 1292
MroXI GAANNNNTTC 2 cut(s) 1519, 1695
MseI TTAA 8 cut(s) 258, 618, 848, 1313, 1613, 1715, 1830, 1836
MslI CAYNNNNRTG 3 cut(s) 1715, 1853, 2157
MspA1I CMGCKG 4 cut(s) 994, 1011, 1562, 2308
MspCI CTTAAG 1 cut(s) 257
MspI CCGG 2 cut(s) 983, 1293
MspR9I CCNGG 3 cut(s) 1215, 1324, 2217
MunI CAATTG 2 cut(s) 1173, 1380
Mva1269I GAATGC 1 cut(s) 1547
MvaI CCWGG 3 cut(s) 1215, 1324, 2217
MvnI CGCG 1 cut(s) 395
MwoI GCNNNNNNNGC 5 cut(s) 30, 555, 1506, 1997, 2006
NaeI GCCGGC 1 cut(s) 1294
NdeI CATATG 2 cut(s) 1225, 2158
NdeII GATC 8 cut(s) 209, 340, 951, 1092, 1586, 1947, 2028, 2161
NgoMIV GCCGGC 1 cut(s) 1292
NlaIV GGNNCC 4 cut(s) 310, 357, 1457, 1930
NmuCI GTSAC 3 cut(s) 925, 1141, 1855
NsiI ATGCAT 1 cut(s) 2158
NspI RCATGY 6 cut(s) 127, 1322, 1647, 1747, 2106, 2156
PaeI GCATGC 2 cut(s) 127, 1322
PaeR7I CTCGAG 1 cut(s) 2139
PagI TCATGA 4 cut(s) 324, 523, 2065, 2224
PciI ACATGT 2 cut(s) 1743, 2102
PciSI GCTCTTC 1 cut(s) 716
PctI GAATGC 1 cut(s) 1547
PdiI GCCGGC 1 cut(s) 1294
PdmI GAANNNNTTC 2 cut(s) 1519, 1695
PfeI GAWTC 8 cut(s) 171, 193, 596, 763, 789, 1421, 1438, 2069
PflMI CCANNNNNTGG 2 cut(s) 34, 2247
Ple19I CGATCG 1 cut(s) 1095
PpuMI RGGWCCY 1 cut(s) 2219
PscI ACATGT 2 cut(s) 1743, 2102
Psp5II RGGWCCY 1 cut(s) 2219
Psp6I CCWGG 3 cut(s) 1213, 1322, 2215
PspFI CCCAGC 1 cut(s) 1459
PspGI CCWGG 3 cut(s) 1213, 1322, 2215
PspN4I GGNNCC 4 cut(s) 310, 357, 1457, 1930
PspPI GGNCC 2 cut(s) 356, 2219
PspPPI RGGWCCY 1 cut(s) 2219
PspXI VCTCGAGB 1 cut(s) 2139
PsrI GAACNNNNNNTAC 2 cut(s) 2127, 2159
PsuI RGATCY 1 cut(s) 340
PvuI CGATCG 1 cut(s) 1095
PvuII CAGCTG 2 cut(s) 994, 1562
RseI CAYNNNNRTG 3 cut(s) 1715, 1853, 2157
SapI GCTCTTC 1 cut(s) 716
SaqAI TTAA 8 cut(s) 258, 618, 848, 1313, 1613, 1715, 1830, 1836
Sau3AI GATC 8 cut(s) 209, 340, 951, 1092, 1586, 1947, 2028, 2161
Sau96I GGNCC 2 cut(s) 356, 2219
ScaI AGTACT 3 cut(s) 415, 820, 1102
ScrFI CCNGG 3 cut(s) 1215, 1324, 2217
SduI GDGCHC 7 cut(s) 635, 917, 1133, 1265, 1511, 1809, 2176
SfaNI GCATC 2 cut(s) 742, 2083
SfcI CTRYAG 3 cut(s) 132, 1557, 2001
Sfr274I CTCGAG 1 cut(s) 2139
SinI GGWCC 2 cut(s) 356, 2219
SlaI CTCGAG 1 cut(s) 2139
SmiMI CAYNNNNRTG 3 cut(s) 1715, 1853, 2157
SmlI CTYRAG 7 cut(s) 74, 257, 344, 853, 1069, 1264, 2139
SmoI CTYRAG 7 cut(s) 74, 257, 344, 853, 1069, 1264, 2139
SpeI ACTAGT 1 cut(s) 284
SphI GCATGC 2 cut(s) 127, 1322
SsiI CCGC 6 cut(s) 128, 306, 1011, 1278, 1795, 2308
SspMI CTAG 5 cut(s) 236, 285, 435, 1104, 2073
StyD4I CCNGG 3 cut(s) 1213, 1322, 2215
StyI CCWWGG 3 cut(s) 1359, 1735, 1810
TaaI ACNGT 9 cut(s) 428, 680, 925, 975, 1141, 1190, 1618, 2084, 2134
TaiI ACGT 1 cut(s) 593
TaqI TCGA 7 cut(s) 230, 439, 787, 1161, 1198, 1424, 2140
TaqII GACCGA 1 cut(s) 2041
TatI WGTACW 7 cut(s) 55, 225, 413, 818, 1100, 2275, 2354
TauI GCSGC 1 cut(s) 130
TfiI GAWTC 8 cut(s) 171, 193, 596, 763, 789, 1421, 1438, 2069
Tru1I TTAA 8 cut(s) 258, 618, 848, 1313, 1613, 1715, 1830, 1836
Tru9I TTAA 8 cut(s) 258, 618, 848, 1313, 1613, 1715, 1830, 1836
TscAI CASTG 2 cut(s) 704, 2293
TseFI GTSAC 3 cut(s) 925, 1141, 1855
Tsp45I GTSAC 3 cut(s) 925, 1141, 1855
TspGWI ACGGA 2 cut(s) 1011, 1256
TspRI CASTG 2 cut(s) 704, 2293
Van91I CCANNNNNTGG 2 cut(s) 34, 2247
Vha464I CTTAAG 1 cut(s) 257
VneI GTGCAC 1 cut(s) 1805
VpaK11BI GGWCC 2 cut(s) 356, 2219
XagI CCTNNNNNAGG 2 cut(s) 1083, 2268
XapI RAATTY 8 cut(s) 13, 277, 539, 668, 858, 1074, 1519, 1832
XbaI TCTAGA 1 cut(s) 2072
XceI RCATGY 6 cut(s) 127, 1322, 1647, 1747, 2106, 2156
XcmI CCANNNNNNNNNTGG 3 cut(s) 895, 1366, 1597
XhoI CTCGAG 1 cut(s) 2139
XmiI GTMKAC 2 cut(s) 1556, 2023
XmnI GAANNNNTTC 2 cut(s) 1519, 1695
XspI CTAG 5 cut(s) 236, 285, 435, 1104, 2073
ZrmI AGTACT 3 cut(s) 415, 820, 1102
Zsp2I ATGCAT 1 cut(s) 2158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.