Prupe.4G181700_v2.0.a1

Protein of unknown function (DUF2921)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000420
Physical Location & Seq
Forward (+)
10784667 .. 10787926
3260 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G181700.1

Sequence Viewer

Length: 2829 bp
ATGTTTGTCTCAAAACAGATGAACAAAGAACCCAAGTGCAAGCCTTCTTCTGTTATCACCACCTCTTTGAAGATGTGGTACAAACTCTCAAAGCTTGTCATATTCTTCTTTGTTTTCACCACACCCTCTGCAGCCTCTTCTAGATTCAACTACCAAACCGCTTACACTGATCATTGTGCTTCATTTGTTCCCGAGTCTGACCCGGAAGGTAACGTGCTAGGACCGCCCTATCAATACCGCCATACAGGTTACTACACTGGTGGTGGTAGTGGTGGCATTCTCAGTCCAAACTCATCAATTGACTTCTACACCAGGAGTATTATAGAAACTAAAGTGCAGGGCTTATTCAAGCTTCAAGGACGCATACGATTTCCAAGAGCCAGCACATATCACTTTGTGGGAAATTCCACAAGCAACAAGTATGGATCAGCTTCTCATCGACGGAGTTCAATAGCTTTTGCACTAGATGGGTTCTGGTCACAATCTTCTGGGAAGCTTTGTATGGTTGGATCAGCTTATGGTTATTTGCGTAATGTTCATTCTGTTCTTAAGCTCTATAATTTCATGAATTCCACTAGTATTACAAGTATGATCAGTGGAACCTTAGAGAGCTTGATGAGGTCTGAGAATGATCCAAATGATTTTGAGCCAATCTCTATTTTGATTTTTCCTAGTATGAACTACCAGTACACTCTGGTTTCAAATAAATCTGAAAACAGGAGTTCTTCTGGTGGAAGTGATGATTCCAATCCCACCAGTTCTTTGAAAATGGAGAGGTTTTGTTCAGTACTCTCAAGCGAAGTACTAAATCATGAATTTGATCTCAAATATTCAAGCGGTTGCGCTTCTGCTAAGAACTGCACTCCACTTGCTGTGTCTGATTTGCCTCGCGTTATGTCGTTGAAGGCTATTGAGTGTTTGGAGGATGAAAGAAGCTTGAGAGTTCTGGTGGAATTTGCAGAGAGTAACAGCCTCTGGTATCGAAGGCCTTTCAATCCCAATAAAACGTTGGTTGGGGAAGGCTCGTGGAATACTGAGAAAAACCAACTATCTGTTGTTGCTTGTCAGTTCTTGGATGCAGCAGGTTCTTGGAATAATGTTCGTGTGGGAAATTGTTCAACAAGATTGAGCTTGAAAATTCCTGCAATATGGACAATCGGAAACACTAGTAGCATTGTGGGGCATATTTGGAGCAACAAAACTGCGACAGAGTCAGGTTACCTTGAACAGATTACATTTGAAAGTCCTCAAGATGACGTTGGAAGGGTTCTGATTCCAGGCTTGAAATACAAGTATACGAAAATGGACAAAGTAACCAAGTTGTGCCCGAGAAAGAAGGCTGCTCATGACAAGGCCAATGTATACCCGAATCCCTTTTCTTATGAAATGCGATTCGATGTGTCAGCTAAAAATTTGAAAGGAGAACTTGCATGGGGCAGTTCTGTCCCACTCTCTGTTGGCAATCAGTTTTACCAGTCGTATTGGTATTCAACTGTAAGCACCAATGAGTCCTCTGTAGGTTTTGCTCCTGTGAGCTCTCCTGTGACTGTGAGCTACAGCAACAACCAGAGTAACCCCTACAATATTAGCTACACAATAAGAATCACATCGCTATCTTATGCAAAGTTGGGGAATGTATCAATTTTAAATGATACGCAGATTTTTGCAGAAGGGATCTATGATGAAACAGAAGGAAGCCTGTGCATGGTTGGCTGCAGAAATCTGGGCTCAAAGAATCAACAGCCAACAAATGATTCAGTAGATTGTGATATTGTTGTCAATTTTCAATTCCCTCCAACAAATCCAAGCAAGAAATGGAGTTTGATCAAGGGAAGCATCAAAAGCACACGAAAAAAGTCCGATCCTCTTCATTTTGAAAGTTGGGATTTGTCTTCAGCTTCCAGTTACCTGGTTGAAGAAAGGCGGTCCATTTGGAGGATGGATGTGGAGATCACCTTGGTTCTTGTATCCACCACACTTTCATGTGTTTTTGTGGCGCTACAACTCTTCCATGTGAAAAAGTACCCTGATGTTCTTCCCTCCATTTCCATTTTCATGCTACTAATTCTTACTCTGGGCTACATGATACCTCTTATGCTAAACTTTGAAGCCATGTTCGCGAATAGCACCAACCGCCGAAGTGTGTTCCTTGGAAGTGGTGGATGGCTTGAAGTTAATGAGGTGATTGTAAGAGTAATAACAATGGTAGCTTTCTTGCTGCAAATCCGTCTTCTGCAGCTAACATGGTCAGCAAGATCAGCAACTGGAACCCAAAAGGAGCTCTGGATCATGGAGAGGAAGACTCTCTTTGTGGTTTTACTAATATATGTTGCTGGGGCTTTGGCTGCTTTGCTTCTGCATACACTGAACTGGAGGAAGAGCCTTAATGATGGTTCCATAACTGCCTATCCAGGGGCAGGCCATCAGCAGCATTCACATTTGGGCACTGCCGTGAAATCTTATGCTGGTTTGGTCTTGGACGGTTTTTTGTTGCCTCAAATTCTGCTCAACATGTTCTGCAAATCAAGGGAAAAGGCTCTTTCTGTTTCATTTTACATCGGAACAACTTTTGTTCGAGCATTGCCACATGCGTATGATCTCTACAGGGCTCACAACTCTGCTCACCATCCGCTAGATGAGTCATACCTTTATGCAAGTCCTGTGGCAGACTTCTACTCTACTGCTTGGGATGTCATTATTCCTCTTGGAGGTTTACTGTTTGCTGGCATTATTTACTTGCAGCAGAGGTTTGGGGGTCTTTGCATTCTTCCCCAAAAGTTGAGAGAGTTGGGTGCATATGAGAAGGTCCCTACTGTTACTGAAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
DUF2921

Protein Analysis

943

Amino Acids

105.01

Weight (kDa)

8.94

Isoelectric Point (pI)

40.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21700
fragaria_vesca FvH4_1g10360 FvH4_1g10760 FvH4_2g30370 FvH4_3g09240 FvH4_3g20050 FvH4_3g20280 FvH4_3g20290 FvH4_3g20291 FvH4_3g20340 FvH4_3g20350 FvH4_3g20351 FvH4_3g20380 FvH4_3g20390 FvH4_5g13190 FvH4_6g20070 FvH4_6g42890
malus_domestica MD03G1227000.v1.1 MD10G1294900.v1.1 MD11G1246300.v1.1 MD11G1246400.v1.1 MD11G1246500.v1.1 MD11G1246600.v1.1
prunus_persica Prupe.4G047300_v2.0.a1 Prupe.4G181700_v2.0.a1 Prupe.4G181900_v2.0.a1 Prupe.4G182000_v2.0.a1 Prupe.4G182200_v2.0.a1
pyrus_communis pycom03g17470 pycom10g24670 pycom11g21640
rosa_chinensis RchiOBHm_Chr5g0008381 RchiOBHm_Chr5g0008391 RchiOBHm_Chr5g0033881 RchiOBHm_Chr5g0034171 RchiOBHm_Chr5g0034181 RchiOBHm_Chr5g0034191 RchiOBHm_Chr5g0034201 RchiOBHm_Chr5g0034211 RchiOBHm_Chr5g0034221 RchiOBHm_Chr5g0034231 RchiOBHm_Chr5g0034271 RchiOBHm_Chr5g0034281
rosa_laevigata RLG00000031566 RLG00000031567 RLG00000033506 RLG00000033529 RLG00000033530 RLG00000033531
rosa_multiflora Rmu_co8445509.1_g000001 Rmu_co8470615.1_g000001 Rmu_sc0008805.1_g000003 Rmu_sc0008805.1_g000004 Rmu_ssc0000128.1_g000029 Rmu_ssc0000128.1_g000031 Rmu_ssc0000128.1_g000032 Rmu_ssc0000388.1_g000062 Rmu_ssc0000388.1_g000063
rosa_roxburghii Rroxscaffold_1G00046010 Rroxscaffold_1G00046020 Rroxscaffold_1G00046030 Rroxscaffold_1G00046040 Rroxscaffold_1G00046060 Rroxscaffold_1G00046070 Rroxscaffold_1G00046080 Rroxscaffold_1G00046090
rosa_rugosa Rorug04G0435500 Rorug05G0142900 Rorug05G0144600 Rorug05G0144600 Rorug05G0144700 Rorug05G0144800
rosa_samantha Rh5AG233800 Rh5AG235900 Rh5AG236000 Rh5AG236100 Rh5AG236200 Rh5AG236300 Rh5AG236500 Rh5BG061200 Rh5BG061300 Rh5BG234300 Rh5BG236600 Rh5BG236800 Rh5CG071200 Rh5DG060000 Rh5DG060100 Rh5DG241300 Rh5DG243700 Rh5DG244000 Rh5DG244100
rosa_wichuraiana Rw3G018950 Rw5G005680 Rw5G021330 Rw5G021340 Rw5G021350 Rw5G021550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1073
AccI GTMKAC 2 cut(s) 1295, 1362
AccII CGCG 2 cut(s) 891, 2120
AciI CCGC 7 cut(s) 159, 224, 238, 837, 1924, 2134, 2630
AclI AACGTT 1 cut(s) 1007
AclWI GGATC 6 cut(s) 433, 517, 626, 1682, 1856, 2294
AcsI RAATTY 7 cut(s) 403, 568, 815, 953, 1137, 1411, 2499
AcuI CTGAAG 1 cut(s) 1878
AdeI CACNNNGTG 1 cut(s) 397
AfaI GTAC 5 cut(s) 80, 689, 789, 804, 2024
AfiI CCNNNNNNNGG 5 cut(s) 1705, 1935, 2412, 2417, 2708
AflII CTTAAG 1 cut(s) 548
AflIII ACRYGT 1 cut(s) 2511
AhlI ACTAGT 2 cut(s) 575, 1166
AjnI CCWGG 4 cut(s) 311, 1276, 1908, 2410
AjuI GAANNNNNNNTTGG 2 cut(s) 1738, 1770
AleI CACNNNNGTG 1 cut(s) 2450
Alw21I GWGCWC 2 cut(s) 1538, 2283
Alw26I GTCTC 1 cut(s) 13
AlwI GGATC 6 cut(s) 433, 517, 626, 1682, 1856, 2294
AlwNI CAGNNNCTG 2 cut(s) 975, 2264
Ama87I CYCGRG 2 cut(s) 191, 1327
AoxI GGCC 3 cut(s) 986, 1353, 2419
ApeKI GCWGC 9 cut(s) 131, 1079, 1340, 1713, 2218, 2236, 2345, 2428, 2740
ApoI RAATTY 7 cut(s) 403, 568, 815, 953, 1137, 1411, 2499
Asp700I GAANNNNTTC 1 cut(s) 1114
AspLEI GCGC 2 cut(s) 845, 1999
AspS9I GGNCC 3 cut(s) 221, 1926, 2806
AsuC2I CCSGG 1 cut(s) 203
AsuHPI GGTGA 5 cut(s) 49, 109, 1945, 2194, 2615
AvaI CYCGRG 2 cut(s) 191, 1327
AvaII GGWCC 3 cut(s) 221, 1926, 2806
BaeGI GKGCMC 2 cut(s) 1328, 2447
BanII GRGCYC 4 cut(s) 1538, 1730, 2283, 2611
BarI GAAGNNNNNNTAC 2 cut(s) 62, 94
BauI CACGAG 1 cut(s) 1024
BbsI GAAGAC 3 cut(s) 1884, 2222, 2306
Bbv12I GWGCWC 2 cut(s) 1538, 2283
BbvI GCAGC 9 cut(s) 143, 1091, 1327, 1700, 2205, 2248, 2332, 2440, 2752
BccI CCATC 6 cut(s) 461, 1933, 2157, 2384, 2430, 2634
BceAI ACGGC 1 cut(s) 2435
BciT130I CCWGG 4 cut(s) 313, 1278, 1910, 2412
BciVI GTATCC 1 cut(s) 1978
BclI TGATCA 3 cut(s) 169, 591, 1824
BcnI CCSGG 1 cut(s) 203
BcoDI GTCTC 1 cut(s) 13
BcuI ACTAGT 2 cut(s) 575, 1166
BfaI CTAG 7 cut(s) 141, 218, 464, 576, 672, 1167, 2633
BfmI CTRYAG 6 cut(s) 129, 1515, 1555, 1714, 2234, 2602
BfoI RGCGCY 1 cut(s) 2000
BfrI CTTAAG 1 cut(s) 548
BfuAI ACCTGC 1 cut(s) 1073
BfuI GTATCC 1 cut(s) 1978
BisI GCNGC 9 cut(s) 132, 1080, 1341, 1714, 2219, 2237, 2346, 2429, 2741
BlsI GCNGC 9 cut(s) 133, 1081, 1342, 1715, 2220, 2238, 2347, 2430, 2742
BmcAI AGTACT 2 cut(s) 789, 804
Bme1390I CCNGG 5 cut(s) 203, 313, 1278, 1910, 2412
Bme18I GGWCC 3 cut(s) 221, 1926, 2806
BmeT110I CYCGRG 2 cut(s) 191, 1327
BmgT120I GGNCC 3 cut(s) 221, 1926, 2806
BmiI GGNNCC 4 cut(s) 601, 2269, 2395, 2808
BmrFI CCNGG 5 cut(s) 203, 313, 1278, 1910, 2412
BmsI GCATC 2 cut(s) 1066, 1845
BpiI GAAGAC 3 cut(s) 1884, 2222, 2306
BplI GAGNNNNNCTC 4 cut(s) 638, 670, 2287, 2319
BpmI CTGGAG 1 cut(s) 2392
BpuEI CTTGAG 3 cut(s) 778, 958, 1233
BpuMI CCSGG 1 cut(s) 203
BsaBI GATNNNNATC 1 cut(s) 747
BsaJI CCNNGG 3 cut(s) 1956, 2149, 2411
Bsc4I CCNNNNNNNGG 5 cut(s) 1705, 1935, 2412, 2417, 2708
Bse1I ACTGG 7 cut(s) 262, 685, 756, 1474, 1902, 2269, 2375
Bse3DI GCAATG 1 cut(s) 2579
Bse8I GATNNNNATC 1 cut(s) 747
BseBI CCWGG 4 cut(s) 313, 1278, 1910, 2412
BseDI CCNNGG 3 cut(s) 1956, 2149, 2411
BseGI GGATG 7 cut(s) 931, 1081, 1944, 1948, 2168, 2626, 2695
BseJI GATNNNNATC 1 cut(s) 747
BseLI CCNNNNNNNGG 5 cut(s) 1705, 1935, 2412, 2417, 2708
BseMI GCAATG 1 cut(s) 2579
BseMII CTCAG 3 cut(s) 295, 615, 1026
BseNI ACTGG 7 cut(s) 262, 685, 756, 1474, 1902, 2269, 2375
BseSI GKGCMC 2 cut(s) 1328, 2447
BseXI GCAGC 9 cut(s) 143, 1091, 1327, 1700, 2205, 2248, 2332, 2440, 2752
BseYI CCCAGC 1 cut(s) 2333
BsgI GTGCAG 2 cut(s) 356, 844
Bsh1236I CGCG 2 cut(s) 891, 2120
BshFI GGCC 3 cut(s) 988, 1355, 2421
BsiHKAI GWGCWC 2 cut(s) 1538, 2283
BsiHKCI CYCGRG 2 cut(s) 191, 1327
BsiSI CCGG 1 cut(s) 203
BslFI GGGAC 2 cut(s) 1430, 2792
BslI CCNNNNNNNGG 5 cut(s) 1705, 1935, 2412, 2417, 2708
BsmAI GTCTC 1 cut(s) 13
BsmFI GGGAC 2 cut(s) 1430, 2792
BsmI GAATGC 3 cut(s) 276, 2431, 2763
BsnI GGCC 3 cut(s) 988, 1355, 2421
BsoBI CYCGRG 2 cut(s) 191, 1327
Bsp1286I GDGCHC 6 cut(s) 1328, 1538, 1730, 2283, 2447, 2611
Bsp68I TCGCGA 1 cut(s) 2120
BspACI CCGC 7 cut(s) 159, 224, 238, 837, 1924, 2134, 2630
BspANI GGCC 3 cut(s) 988, 1355, 2421
BspCNI CTCAG 3 cut(s) 294, 616, 1027
BspFNI CGCG 2 cut(s) 891, 2120
BspHI TCATGA 3 cut(s) 564, 811, 1345
BspLI GGNNCC 4 cut(s) 601, 2269, 2395, 2808
BspMAI CTGCAG 3 cut(s) 133, 1718, 2238
BspMI ACCTGC 1 cut(s) 1073
BspPI GGATC 6 cut(s) 433, 517, 626, 1682, 1856, 2294
BspQI GCTCTTC 1 cut(s) 2372
BspTI CTTAAG 1 cut(s) 548
BsrDI GCAATG 1 cut(s) 2579
BsrI ACTGG 7 cut(s) 262, 685, 756, 1474, 1902, 2269, 2375
BssECI CCNNGG 3 cut(s) 1956, 2149, 2411
BssNAI GTATAC 2 cut(s) 1296, 1363
BssSI CACGAG 1 cut(s) 1024
BssT1I CCWWGG 2 cut(s) 1956, 2149
Bst1107I GTATAC 2 cut(s) 1296, 1363
Bst2BI CACGAG 1 cut(s) 1024
Bst2UI CCWGG 4 cut(s) 313, 1278, 1910, 2412
Bst4CI ACNGT 5 cut(s) 1495, 1549, 2483, 2718, 2815
Bst6I CTCTTC 4 cut(s) 142, 1872, 2012, 2372
BstAFI CTTAAG 1 cut(s) 548
BstC8I GCNNGC 4 cut(s) 41, 382, 2419, 2725
BstDEI CTNAG 5 cut(s) 281, 604, 624, 852, 1035
BstEII GGTNACC 1 cut(s) 1217
BstENI CCTNNNNNAGG 1 cut(s) 2706
BstF5I GGATG 7 cut(s) 931, 1081, 1944, 1948, 2168, 2626, 2695
BstFNI CGCG 2 cut(s) 891, 2120
BstH2I RGCGCY 1 cut(s) 2000
BstHHI GCGC 2 cut(s) 845, 1999
BstMAI GTCTC 1 cut(s) 13
BstMWI GCNNNNNNNGC 7 cut(s) 223, 1710, 1842, 2117, 2133, 2258, 2345
BstNI CCWGG 4 cut(s) 313, 1278, 1910, 2412
BstNSI RCATGY 2 cut(s) 2515, 2591
BstPI GGTNACC 1 cut(s) 1217
BstSCI CCNGG 5 cut(s) 201, 311, 1276, 1908, 2410
BstSFI CTRYAG 6 cut(s) 129, 1515, 1555, 1714, 2234, 2602
BstSLI GKGCMC 2 cut(s) 1328, 2447
BstUI CGCG 2 cut(s) 891, 2120
BstV1I GCAGC 9 cut(s) 143, 1091, 1327, 1700, 2205, 2248, 2332, 2440, 2752
BstV2I GAAGAC 3 cut(s) 1884, 2222, 2306
BstX2I RGATCY 1 cut(s) 1674
BstXI CCANNNNNNTGG 1 cut(s) 1909
BstYI RGATCY 1 cut(s) 1674
BstZ17I GTATAC 2 cut(s) 1296, 1363
BsuI GTATCC 1 cut(s) 1978
BsuRI GGCC 3 cut(s) 988, 1355, 2421
BtgZI GCGATG 1 cut(s) 1593
BtsCI GGATG 7 cut(s) 931, 1081, 1944, 1948, 2168, 2626, 2695
BtsI GCAGTG 1 cut(s) 2445
BtsIMutI CAGTG 5 cut(s) 165, 255, 601, 2363, 2445
BtuMI TCGCGA 1 cut(s) 2120
BveI ACCTGC 1 cut(s) 1073
Cac8I GCNNGC 4 cut(s) 41, 382, 2419, 2725
CaiI CAGNNNCTG 2 cut(s) 975, 2264
CciI TCATGA 3 cut(s) 564, 811, 1345
CfoI GCGC 2 cut(s) 845, 1999
Cfr13I GGNCC 3 cut(s) 221, 1926, 2806
CseI GACGC 1 cut(s) 369
CsiI ACCWGGT 1 cut(s) 1908
Csp6I GTAC 5 cut(s) 79, 688, 788, 803, 2023
CspCI CAANNNNNGTGG 2 cut(s) 2643, 2678
CviQI GTAC 5 cut(s) 79, 688, 788, 803, 2023
DdeI CTNAG 5 cut(s) 281, 604, 624, 852, 1035
DraI TTTAAA 1 cut(s) 1647
DraIII CACNNNGTG 1 cut(s) 397
Eam1104I CTCTTC 4 cut(s) 142, 1872, 2012, 2372
EarI CTCTTC 4 cut(s) 142, 1872, 2012, 2372
Ecl136II GAGCTC 2 cut(s) 1536, 2281
Eco130I CCWWGG 2 cut(s) 1956, 2149
Eco147I AGGCCT 1 cut(s) 988
Eco24I GRGCYC 4 cut(s) 1538, 1730, 2283, 2611
Eco47I GGWCC 3 cut(s) 221, 1926, 2806
Eco53kI GAGCTC 2 cut(s) 1536, 2281
Eco57I CTGAAG 1 cut(s) 1878
Eco88I CYCGRG 2 cut(s) 191, 1327
Eco91I GGTNACC 1 cut(s) 1217
EcoICRI GAGCTC 2 cut(s) 1536, 2281
EcoNI CCTNNNNNAGG 1 cut(s) 2706
EcoO109I RGGNCCY 1 cut(s) 2806
EcoO65I GGTNACC 1 cut(s) 1217
EcoRI GAATTC 1 cut(s) 568
EcoRII CCWGG 4 cut(s) 311, 1276, 1908, 2410
EcoT14I CCWWGG 2 cut(s) 1956, 2149
EcoT38I GRGCYC 4 cut(s) 1538, 1730, 2283, 2611
ErhI CCWWGG 2 cut(s) 1956, 2149
FalI AAGNNNNNCTT 4 cut(s) 1410, 1442, 2291, 2323
FaqI GGGAC 2 cut(s) 1430, 2792
FauNDI CATATG 1 cut(s) 2797
FbaI TGATCA 3 cut(s) 169, 591, 1824
FblI GTMKAC 2 cut(s) 1295, 1362
Fnu4HI GCNGC 9 cut(s) 132, 1080, 1341, 1714, 2219, 2237, 2346, 2429, 2741
FokI GGATG 7 cut(s) 938, 1088, 1951, 1955, 2175, 2613, 2702
FriOI GRGCYC 4 cut(s) 1538, 1730, 2283, 2611
Fsp4HI GCNGC 9 cut(s) 132, 1080, 1341, 1714, 2219, 2237, 2346, 2429, 2741
FspBI CTAG 7 cut(s) 141, 218, 464, 576, 672, 1167, 2633
GlaI GCGC 2 cut(s) 844, 1998
GluI GCNGC 9 cut(s) 132, 1080, 1341, 1714, 2219, 2237, 2346, 2429, 2741
GsaI CCCAGC 1 cut(s) 2337
GsuI CTGGAG 1 cut(s) 2392
HaeII RGCGCY 1 cut(s) 2000
HaeIII GGCC 3 cut(s) 988, 1355, 2421
HapII CCGG 1 cut(s) 203
HgaI GACGC 1 cut(s) 369
HhaI GCGC 2 cut(s) 845, 1999
Hin6I GCGC 2 cut(s) 843, 1997
HinP1I GCGC 2 cut(s) 843, 1997
HindIII AAGCTT 4 cut(s) 92, 350, 494, 934
HpaII CCGG 1 cut(s) 203
HphI GGTGA 5 cut(s) 49, 109, 1945, 2194, 2615
Hpy166II GTNNAC 4 cut(s) 690, 1296, 1363, 2714
Hpy188I TCNGA 8 cut(s) 199, 625, 712, 880, 1160, 1272, 1861, 2561
Hpy188III TCNNGA 8 cut(s) 141, 191, 565, 812, 1250, 1346, 2119, 2284
Hpy8I GTNNAC 4 cut(s) 690, 1296, 1363, 2714
Hpy99I CGWCG 1 cut(s) 444
HpyCH4III ACNGT 5 cut(s) 1495, 1549, 2483, 2718, 2815
HpyCH4IV ACGT 3 cut(s) 213, 1007, 1257
HpyF10VI GCNNNNNNNGC 7 cut(s) 223, 1710, 1842, 2117, 2133, 2258, 2345
HpyF3I CTNAG 5 cut(s) 281, 604, 624, 852, 1035
HpySE526I ACGT 3 cut(s) 213, 1007, 1257
HspAI GCGC 2 cut(s) 843, 1997
Ksp22I TGATCA 3 cut(s) 169, 591, 1824
LguI GCTCTTC 1 cut(s) 2372
LmnI GCTCC 3 cut(s) 1191, 1531, 2278
Lsp1109I GCAGC 9 cut(s) 143, 1091, 1327, 1700, 2205, 2248, 2332, 2440, 2752
LweI GCATC 2 cut(s) 1066, 1845
MabI ACCWGGT 1 cut(s) 1908
MaeI CTAG 7 cut(s) 141, 218, 464, 576, 672, 1167, 2633
MaeII ACGT 3 cut(s) 213, 1007, 1257
MfeI CAATTG 1 cut(s) 297
MflI RGATCY 1 cut(s) 1674
MhlI GDGCHC 6 cut(s) 1328, 1538, 1730, 2283, 2447, 2611
MlyI GAGTC 5 cut(s) 203, 1220, 1517, 2296, 2648
MmeI TCCRAC 3 cut(s) 487, 1240, 1820
MroXI GAANNNNTTC 1 cut(s) 1114
MseI TTAA 4 cut(s) 549, 1646, 2175, 2385
MslI CAYNNNNRTG 4 cut(s) 1981, 2054, 2450, 2592
MspCI CTTAAG 1 cut(s) 548
MspI CCGG 1 cut(s) 203
MspR9I CCNGG 5 cut(s) 203, 313, 1278, 1910, 2412
MunI CAATTG 1 cut(s) 297
Mva1269I GAATGC 3 cut(s) 276, 2431, 2763
MvaI CCWGG 4 cut(s) 313, 1278, 1910, 2412
MvnI CGCG 2 cut(s) 891, 2120
MwoI GCNNNNNNNGC 7 cut(s) 223, 1710, 1842, 2117, 2133, 2258, 2345
NciI CCSGG 1 cut(s) 203
NdeI CATATG 1 cut(s) 2797
NlaIV GGNNCC 4 cut(s) 601, 2269, 2395, 2808
NmuCI GTSAC 2 cut(s) 477, 1543
NruI TCGCGA 1 cut(s) 2120
NspI RCATGY 2 cut(s) 2515, 2591
OliI CACNNNNGTG 1 cut(s) 2450
PagI TCATGA 3 cut(s) 564, 811, 1345
PceI AGGCCT 1 cut(s) 988
PciI ACATGT 1 cut(s) 2511
PciSI GCTCTTC 1 cut(s) 2372
PctI GAATGC 3 cut(s) 276, 2431, 2763
PdmI GAANNNNTTC 1 cut(s) 1114
PfeI GAWTC 8 cut(s) 144, 743, 1273, 1369, 1392, 1602, 1735, 1754
PflFI GACNNNGTC 1 cut(s) 1210
PkrI GCNGC 9 cut(s) 133, 1081, 1342, 1715, 2220, 2238, 2347, 2430, 2742
PleI GAGTC 5 cut(s) 202, 1219, 1516, 2296, 2647
PpsI GAGTC 5 cut(s) 202, 1219, 1516, 2296, 2647
PpuMI RGGWCCY 1 cut(s) 2806
PscI ACATGT 1 cut(s) 2511
Psp124BI GAGCTC 2 cut(s) 1538, 2283
Psp1406I AACGTT 1 cut(s) 1007
Psp5II RGGWCCY 1 cut(s) 2806
Psp6I CCWGG 4 cut(s) 311, 1276, 1908, 2410
PspEI GGTNACC 1 cut(s) 1217
PspFI CCCAGC 1 cut(s) 2333
PspGI CCWGG 4 cut(s) 311, 1276, 1908, 2410
PspN4I GGNNCC 4 cut(s) 601, 2269, 2395, 2808
PspPI GGNCC 3 cut(s) 221, 1926, 2806
PspPPI RGGWCCY 1 cut(s) 2806
PsrI GAACNNNNNNTAC 2 cut(s) 671, 703
PstI CTGCAG 3 cut(s) 133, 1718, 2238
PstNI CAGNNNCTG 2 cut(s) 975, 2264
PsuI RGATCY 1 cut(s) 1674
PsyI GACNNNGTC 1 cut(s) 1210
RruI TCGCGA 1 cut(s) 2120
RsaI GTAC 5 cut(s) 80, 689, 789, 804, 2024
RsaNI GTAC 5 cut(s) 79, 688, 788, 803, 2023
RseI CAYNNNNRTG 4 cut(s) 1981, 2054, 2450, 2592
SacI GAGCTC 2 cut(s) 1538, 2283
SapI GCTCTTC 1 cut(s) 2372
SaqAI TTAA 4 cut(s) 549, 1646, 2175, 2385
SatI GCNGC 9 cut(s) 132, 1080, 1341, 1714, 2219, 2237, 2346, 2429, 2741
Sau96I GGNCC 3 cut(s) 221, 1926, 2806
ScaI AGTACT 2 cut(s) 789, 804
SchI GAGTC 5 cut(s) 203, 1220, 1517, 2296, 2648
ScrFI CCNGG 5 cut(s) 203, 313, 1278, 1910, 2412
SduI GDGCHC 6 cut(s) 1328, 1538, 1730, 2283, 2447, 2611
SexAI ACCWGGT 1 cut(s) 1908
SfaNI GCATC 2 cut(s) 1066, 1845
SfcI CTRYAG 6 cut(s) 129, 1515, 1555, 1714, 2234, 2602
SinI GGWCC 3 cut(s) 221, 1926, 2806
SmiMI CAYNNNNRTG 4 cut(s) 1981, 2054, 2450, 2592
SmlI CTYRAG 4 cut(s) 548, 793, 937, 1248
SmoI CTYRAG 4 cut(s) 548, 793, 937, 1248
SpeI ACTAGT 2 cut(s) 575, 1166
SseBI AGGCCT 1 cut(s) 988
SsiI CCGC 7 cut(s) 159, 224, 238, 837, 1924, 2134, 2630
SspI AATATT 2 cut(s) 830, 1585
SspMI CTAG 7 cut(s) 141, 218, 464, 576, 672, 1167, 2633
SstI GAGCTC 2 cut(s) 1538, 2283
StuI AGGCCT 1 cut(s) 988
StyD4I CCNGG 5 cut(s) 201, 311, 1276, 1908, 2410
StyI CCWWGG 2 cut(s) 1956, 2149
TaaI ACNGT 5 cut(s) 1495, 1549, 2483, 2718, 2815
TaiI ACGT 3 cut(s) 216, 1010, 1260
TaqI TCGA 4 cut(s) 439, 982, 1395, 2575
TatI WGTACW 3 cut(s) 687, 787, 802
TfiI GAWTC 8 cut(s) 144, 743, 1273, 1369, 1392, 1602, 1735, 1754
Tru1I TTAA 4 cut(s) 549, 1646, 2175, 2385
Tru9I TTAA 4 cut(s) 549, 1646, 2175, 2385
TscAI CASTG 5 cut(s) 172, 262, 601, 2370, 2452
TseFI GTSAC 2 cut(s) 477, 1543
TseI GCWGC 9 cut(s) 131, 1079, 1340, 1713, 2218, 2236, 2345, 2428, 2740
Tsp45I GTSAC 2 cut(s) 477, 1543
TspGWI ACGGA 2 cut(s) 457, 2216
TspRI CASTG 5 cut(s) 172, 262, 601, 2370, 2452
Tth111I GACNNNGTC 1 cut(s) 1210
Vha464I CTTAAG 1 cut(s) 548
VpaK11BI GGWCC 3 cut(s) 221, 1926, 2806
XagI CCTNNNNNAGG 1 cut(s) 2706
XapI RAATTY 7 cut(s) 403, 568, 815, 953, 1137, 1411, 2499
XbaI TCTAGA 1 cut(s) 140
XceI RCATGY 2 cut(s) 2515, 2591
XcmI CCANNNNNNNNNTGG 3 cut(s) 1006, 1812, 1936
XmiI GTMKAC 2 cut(s) 1295, 1362
XmnI GAANNNNTTC 1 cut(s) 1114
XspI CTAG 7 cut(s) 141, 218, 464, 576, 672, 1167, 2633
ZrmI AGTACT 2 cut(s) 789, 804
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.