Rroxscaffold_1G00046020

Protein of unknown function (DUF2921)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000420
Physical Location & Seq
Forward (+)
65177158 .. 65179863
2706 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00046020.1

Sequence Viewer

Length: 2706 bp
ATGACAATCTCCATGAAGCTTTTCATATCCTTCTTTGTTTTTACCACCATATCCTTCAAGTCGATGTCATTCTTTGCTTCAGCTGTTCAAATTTCTTATGCTGACCATTGTGCTTCAGTTGTTCCCGAGTCAACCGAAAAAATAAATGCACGATCTCACTTGGTACATCTGATTCACACAGGCTACTACTACACTAGTGGTGGCAGTGACATTCTTAATCTATACTCGCCAAATGATCAGGTCAAAAATTCTATTGGCTTCCAATGCTGGGACATTAGAGAAACTAATGTACAAGGCTTGTTCAAGGTTGAAGGAACTCTTGAGTTTCAAGTAACCCACAGGCATGAGCAGCAAAGCCTGCCAGTTCAAACATTTCCATGTTCCGTTTCCTTTAGACTAAATGGATTCTGGTCAGAATCTTCTGGGAAGCTTTGCATGGTCGGATCATCGTCCATTTACTCAGCACAAGGTAATCTTCTTGTTCTTAAGCTGTACAATCTCATGAATTCCACTAGTGTTACAAGTTTGATTAGTGGAACCTTGGAGCATGTCATGATATCTGAGAATGATCCCAAGTATTTAGGACCAATCTCTATTTTGCTATTCCCTCGCATGAACTACGAGTACACTTTGGTCTCAACTAATTATAATAACAGTTGTTCTGGTGGAAGTGAAGATACCCCAAGCTCTTTACAAGTAGAGAGATTTTGTTCACTACTATCATCAGTGGTACTAAATCATGACTTTCAACTCAAATATTCAAGCCACTGTGTTACTGCAAAGAACTGCAGCCCAATTTCTATGTCTGATAGGCCTCGGGCTGTATCGTTGAAGGACATTGTGTGTTCAGAGGATAAACGAAGGGTGAGAGTTCTGGTAGAATTTGTAGACGGTTGGCCTAACTGGCATCCAAGGCTCTTCAATCCTAGTACAAGTTTAGTTGGTGAAGGCTCATGGGATGCAGAGAAGAATCAGCTATGTATCATTGCTTGTCGAATCTTGAATGAAACAAATTCTTTGAATAATACTAATGTGGGTGATTGTTCAACAAGATTGAGCTTGAGATTTCCTGCAATAGGGACAATTGGAAGTATCAGCAGCATTGTGGGGCATATTTGGAGCACCAAAACTGTGACAGAGTTGGGCTACTTTGAAAAGATCACATTTGCCAGTGGGGACTGTAGTTATCAAGAGTGTCTGCTTCCAGGCCAGACATACGAGTATACTAAAATGGACAAAGTAACAGAGTTGTGCTCGAGAAAGAAGGCTGCTAATGACAAGGCCAGTATTTACCCAAATCTGTTGTTGCATGGCATGAGTTTCATTATGCATGCTGAAAATTCTGAAGGACAAGTTGGTACAGGCAATGGTGTTCCTCTTTCTGTTGGCGATCAATTTTACAAGACCGACTATTCAACTGTAAGCAATCAAGAATACTCTTTGGCTCCTCTAAGTCACAGCTACAACCACAGCAGCTCCTACAATATTAGCTACAAAATAGACCTCCATCTACAATCAAATCCAGCGTTGGGCAAACAGTCAGTTATATATGATATGCACATCTCTGCTGAAGGGATCTATGATGAAACAGAAGGAAGCCTGTGCATGGTAGGATGCCGCAATCTTGGCTCAAACAGTCAGCAGGCAACAAATGATGTAATAGATTGTGAGATTCTTGTCAATTTTCAGTTCCCACCAACAAATGCAAAGGATTCACATCATATCAAAGGAAGCATTGAAAGCACACGAAAGAAATCTGATCCTCTTCATTTTGAACGCTTGGATTTGTCTACAGCTTCCAAAGATGTAGGAAAGCGACCTATTTGGAGGATGGATGGGAAGATTACCTTCGTTCTTATATCCACCACACTTGCATGTGTTTTTGCTGCATTACAACTCTTCCATGTAAAAAGAAATCCTGAAGTCATTCCCTCTATTTCCATCTTAATGCATCTAATGCTAACCCTTGGCTACATGATACCTCTGGGGATAAATTTTCGAGCCATGTTCACACATGATATAAACAGCCAAAATGTGTACCTTGGAGGCGGTGGATGGCTTGAATATAATGAGATAATCATAAGAATAACAACATTGGTAGCTTTCTTGATGCAATTCCACCTTCTGAAGCTTACTTGGTCAGCAAAATCGGGAAATAGAACCCAAAAGGATCTCCAGGTCATGGAAAAGAAGGCTCTGTTTGTGGCTTTACAAGTGTATGCCGTAGGGGCTTTAGCAGCTTTGTTTTTGCATAGGCAGAACCTGAGGAAGAGGGACAATGGCAATGTGTTGCATTATGTAGAGTATTCCATCTTGGATGCCTTGATATCTTATGGTGGTCTGGTCTTGGGTGGTTTTTTGTTCCCTCAAATTCTGCTCAACATGTTCTGCAAATCAAGAGAAAATGCTCTATCAGTCTGGTTCTACTTTGGAACAACTTTTGTTCAAGCGCTGCCACATGCATATGACCTTTACAGGGCTCACAGCTATGCTGGTGACCATTTGGACAAGTACTCTCACATTGATCCAAGTCGTGCGGCAGATTATTTTCACTCCACAGCTTGGAATGCCATTGCAAGCAGCGGAGGTCTACTGTTTGCTAGTATCATTTACTTACAGCAGCAGTTTGGGGGTCGTTGCATTGCTCCTCGAAAGTTGCAAGAGTTGGGTGTATTTGAGAAGGTCCCTACAATTCATGAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
DUF2921

Protein Analysis

901

Amino Acids

100.76

Weight (kDa)

6.79

Isoelectric Point (pI)

38.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2921_N PF25333 33 - 198 8.1e-18 DUF2921 lipocalin-like domain
DUF2921_N PF25333 236 - 388 8.5e-28 DUF2921 lipocalin-like domain
DUF2921_N PF25333 419 - 595 3.4e-34 DUF2921 lipocalin-like domain
DUF2921 PF11145 607 - 885 1e-54 Transmembrane E3 ligase/DUF2921 transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21700
fragaria_vesca FvH4_1g10360 FvH4_1g10760 FvH4_2g30370 FvH4_3g09240 FvH4_3g20050 FvH4_3g20280 FvH4_3g20290 FvH4_3g20291 FvH4_3g20340 FvH4_3g20350 FvH4_3g20351 FvH4_3g20380 FvH4_3g20390 FvH4_5g13190 FvH4_6g20070 FvH4_6g42890
malus_domestica MD03G1227000.v1.1 MD10G1294900.v1.1 MD11G1246300.v1.1 MD11G1246400.v1.1 MD11G1246500.v1.1 MD11G1246600.v1.1
prunus_persica Prupe.4G047300_v2.0.a1 Prupe.4G181700_v2.0.a1 Prupe.4G181900_v2.0.a1 Prupe.4G182000_v2.0.a1 Prupe.4G182200_v2.0.a1
pyrus_communis pycom03g17470 pycom10g24670 pycom11g21640
rosa_chinensis RchiOBHm_Chr5g0008381 RchiOBHm_Chr5g0008391 RchiOBHm_Chr5g0033881 RchiOBHm_Chr5g0034171 RchiOBHm_Chr5g0034181 RchiOBHm_Chr5g0034191 RchiOBHm_Chr5g0034201 RchiOBHm_Chr5g0034211 RchiOBHm_Chr5g0034221 RchiOBHm_Chr5g0034231 RchiOBHm_Chr5g0034271 RchiOBHm_Chr5g0034281
rosa_laevigata RLG00000031566 RLG00000031567 RLG00000033506 RLG00000033529 RLG00000033530 RLG00000033531
rosa_multiflora Rmu_co8445509.1_g000001 Rmu_co8470615.1_g000001 Rmu_sc0008805.1_g000003 Rmu_sc0008805.1_g000004 Rmu_ssc0000128.1_g000029 Rmu_ssc0000128.1_g000031 Rmu_ssc0000128.1_g000032 Rmu_ssc0000388.1_g000062 Rmu_ssc0000388.1_g000063
rosa_roxburghii Rroxscaffold_1G00046010 Rroxscaffold_1G00046020 Rroxscaffold_1G00046030 Rroxscaffold_1G00046040 Rroxscaffold_1G00046060 Rroxscaffold_1G00046070 Rroxscaffold_1G00046080 Rroxscaffold_1G00046090
rosa_rugosa Rorug04G0435500 Rorug05G0142900 Rorug05G0144600 Rorug05G0144600 Rorug05G0144700 Rorug05G0144800
rosa_samantha Rh5AG233800 Rh5AG235900 Rh5AG236000 Rh5AG236100 Rh5AG236200 Rh5AG236300 Rh5AG236500 Rh5BG061200 Rh5BG061300 Rh5BG234300 Rh5BG236600 Rh5BG236800 Rh5CG071200 Rh5DG060000 Rh5DG060100 Rh5DG241300 Rh5DG243700 Rh5DG244000 Rh5DG244100
rosa_wichuraiana Rw3G018950 Rw5G005680 Rw5G021330 Rw5G021340 Rw5G021350 Rw5G021550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 648
AccB7I CCANNNNNTGG 2 cut(s) 2182, 2562
AccI GTMKAC 4 cut(s) 888, 1223, 1790, 2590
AciI CCGC 4 cut(s) 1618, 2049, 2537, 2583
AclWI GGATC 6 cut(s) 451, 563, 1583, 1754, 2178, 2519
AcsI RAATTY 8 cut(s) 90, 247, 505, 881, 1012, 1339, 1993, 2370
AcuI CTGAAG 6 cut(s) 63, 99, 1365, 1590, 1941, 2147
AfaI GTAC 9 cut(s) 165, 291, 494, 626, 732, 931, 1360, 2039, 2513
AfeI AGCGCT 1 cut(s) 2451
AfiI CCNNNNNNNGG 7 cut(s) 268, 1076, 1529, 1606, 2182, 2476, 2562
AflII CTTAAG 1 cut(s) 485
AflIII ACRYGT 1 cut(s) 2382
AhlI ACTAGT 2 cut(s) 194, 512
AjnI CCWGG 2 cut(s) 1204, 2175
AjuI GAANNNNNNNTTGG 2 cut(s) 1338, 1370
Alw21I GWGCWC 2 cut(s) 1124, 1256
Alw26I GTCTC 1 cut(s) 640
AlwI GGATC 6 cut(s) 451, 563, 1583, 1754, 2178, 2519
AlwNI CAGNNNCTG 1 cut(s) 2263
Ama87I CYCGRG 3 cut(s) 125, 816, 1255
Aor51HI AGCGCT 1 cut(s) 2451
AoxI GGCC 4 cut(s) 812, 896, 1207, 1281
ApoI RAATTY 8 cut(s) 90, 247, 505, 881, 1012, 1339, 1993, 2370
Asp700I GAANNNNTTC 2 cut(s) 20, 1926
AspLEI GCGC 1 cut(s) 2452
AspS9I GGNCC 2 cut(s) 584, 2683
AsuHPI GGTGA 4 cut(s) 877, 956, 1049, 2507
AvaI CYCGRG 3 cut(s) 125, 816, 1255
AvaII GGWCC 2 cut(s) 584, 2683
AxyI CCTNAGG 1 cut(s) 2264
BanII GRGCYC 1 cut(s) 2482
BarI GAAGNNNNNNTAC 2 cut(s) 661, 693
Bbv12I GWGCWC 2 cut(s) 1124, 1256
BccI CCATC 6 cut(s) 1515, 1825, 1829, 1949, 2049, 2318
BceAI ACGGC 1 cut(s) 2207
BciT130I CCWGG 2 cut(s) 1206, 2177
BclI TGATCA 1 cut(s) 235
BcoDI GTCTC 1 cut(s) 640
BcuI ACTAGT 2 cut(s) 194, 512
BfaI CTAG 4 cut(s) 195, 513, 927, 2601
BfmI CTRYAG 3 cut(s) 787, 1180, 1791
BfoI RGCGCY 1 cut(s) 2453
BfrI CTTAAG 1 cut(s) 485
BglI GCCNNNNNGGC 2 cut(s) 904, 2228
BmcAI AGTACT 1 cut(s) 2513
Bme1390I CCNGG 2 cut(s) 1206, 2177
Bme18I GGWCC 2 cut(s) 584, 2683
BmeT110I CYCGRG 3 cut(s) 125, 816, 1255
BmgT120I GGNCC 2 cut(s) 584, 2683
BmiI GGNNCC 3 cut(s) 538, 1446, 2685
BmrFI CCNGG 2 cut(s) 1206, 2177
BmsI GCATC 6 cut(s) 916, 949, 1604, 1960, 2100, 2308
BplI GAGNNNNNCTC 2 cut(s) 1238, 1270
BpmI CTGGAG 1 cut(s) 2159
BpuEI CTTGAG 2 cut(s) 341, 1081
BsaBI GATNNNNATC 1 cut(s) 1716
BsaI GGTCTC 1 cut(s) 640
BsaJI CCNNGG 5 cut(s) 540, 815, 911, 1966, 2041
BsaXI ACNNNNNCTCC 2 cut(s) 1460, 1490
Bsc4I CCNNNNNNNGG 7 cut(s) 268, 1076, 1529, 1606, 2182, 2476, 2562
Bse1I ACTGG 4 cut(s) 362, 908, 1170, 1284
Bse21I CCTNAGG 1 cut(s) 2264
Bse3DI GCAATG 5 cut(s) 984, 1372, 2290, 2571, 2640
Bse8I GATNNNNATC 1 cut(s) 1716
BseBI CCWGG 2 cut(s) 1206, 2177
BseDI CCNNGG 5 cut(s) 540, 815, 911, 1966, 2041
BseGI GGATG 7 cut(s) 907, 964, 1619, 1836, 1840, 2060, 2323
BseJI GATNNNNATC 1 cut(s) 1716
BseLI CCNNNNNNNGG 7 cut(s) 268, 1076, 1529, 1606, 2182, 2476, 2562
BseMI GCAATG 5 cut(s) 984, 1372, 2290, 2571, 2640
BseMII CTCAG 3 cut(s) 474, 552, 2255
BseNI ACTGG 4 cut(s) 362, 908, 1170, 1284
BseRI GAGGAG 2 cut(s) 1437, 2637
BseYI CCCAGC 1 cut(s) 267
BshFI GGCC 4 cut(s) 814, 898, 1209, 1283
BsiHKAI GWGCWC 2 cut(s) 1124, 1256
BsiHKCI CYCGRG 3 cut(s) 125, 816, 1255
BslFI GGGAC 5 cut(s) 284, 1093, 1190, 2288, 2669
BslI CCNNNNNNNGG 7 cut(s) 268, 1076, 1529, 1606, 2182, 2476, 2562
BsmAI GTCTC 1 cut(s) 640
BsmFI GGGAC 5 cut(s) 284, 1093, 1190, 2288, 2669
BsmI GAATGC 1 cut(s) 2572
BsnI GGCC 4 cut(s) 814, 898, 1209, 1283
Bso31I GGTCTC 1 cut(s) 640
BsoBI CYCGRG 3 cut(s) 125, 816, 1255
Bsp1286I GDGCHC 3 cut(s) 1124, 1256, 2482
Bsp1407I TGTACA 2 cut(s) 289, 492
BspACI CCGC 4 cut(s) 1618, 2049, 2537, 2583
BspANI GGCC 4 cut(s) 814, 898, 1209, 1283
BspCNI CTCAG 3 cut(s) 473, 553, 2256
BspHI TCATGA 4 cut(s) 501, 552, 739, 2695
BspLI GGNNCC 3 cut(s) 538, 1446, 2685
BspMAI CTGCAG 1 cut(s) 791
BspPI GGATC 6 cut(s) 451, 563, 1583, 1754, 2178, 2519
BspQI GCTCTTC 1 cut(s) 923
BspTI CTTAAG 1 cut(s) 485
BspTNI GGTCTC 1 cut(s) 640
BsrDI GCAATG 5 cut(s) 984, 1372, 2290, 2571, 2640
BsrGI TGTACA 2 cut(s) 289, 492
BsrI ACTGG 4 cut(s) 362, 908, 1170, 1284
BssECI CCNNGG 5 cut(s) 540, 815, 911, 1966, 2041
BssNAI GTATAC 1 cut(s) 1224
BssT1I CCWWGG 4 cut(s) 540, 911, 1966, 2041
Bst1107I GTATAC 1 cut(s) 1224
Bst2UI CCWGG 2 cut(s) 1206, 2177
Bst4CI ACNGT 9 cut(s) 656, 770, 893, 1132, 1181, 1420, 1539, 1637, 2595
Bst6I CTCTTC 5 cut(s) 923, 1770, 1904, 2264, 2694
BstAFI CTTAAG 1 cut(s) 485
BstAPI GCANNNNNTGC 2 cut(s) 358, 1957
BstAUI TGTACA 2 cut(s) 289, 492
BstC8I GCNNGC 4 cut(s) 359, 1332, 1644, 2578
BstDEI CTNAG 4 cut(s) 460, 561, 1451, 2264
BstEII GGTNACC 1 cut(s) 2495
BstENI CCTNNNNNAGG 1 cut(s) 1074
BstF5I GGATG 7 cut(s) 907, 964, 1619, 1836, 1840, 2060, 2323
BstH2I RGCGCY 1 cut(s) 2453
BstHHI GCGC 1 cut(s) 2452
BstMAI GTCTC 1 cut(s) 640
BstNI CCWGG 2 cut(s) 1206, 2177
BstNSI RCATGY 5 cut(s) 551, 1334, 1878, 2386, 2462
BstPI GGTNACC 1 cut(s) 2495
BstSCI CCNGG 2 cut(s) 1204, 2175
BstSFI CTRYAG 3 cut(s) 787, 1180, 1791
BstX2I RGATCY 2 cut(s) 1575, 2170
BstYI RGATCY 2 cut(s) 1575, 2170
BstZ17I GTATAC 1 cut(s) 1224
Bsu36I CCTNAGG 1 cut(s) 2264
BsuRI GGCC 4 cut(s) 814, 898, 1209, 1283
BtsCI GGATG 7 cut(s) 907, 964, 1619, 1836, 1840, 2060, 2323
BtsI GCAGTG 1 cut(s) 211
BtsIMutI CAGTG 4 cut(s) 211, 732, 766, 1177
Cac8I GCNNGC 4 cut(s) 359, 1332, 1644, 2578
CaiI CAGNNNCTG 1 cut(s) 2263
CciI TCATGA 4 cut(s) 501, 552, 739, 2695
CfoI GCGC 1 cut(s) 2452
Cfr13I GGNCC 2 cut(s) 584, 2683
Csp6I GTAC 9 cut(s) 164, 290, 493, 625, 731, 930, 1359, 2038, 2512
CspCI CAANNNNNGTGG 2 cut(s) 1852, 1887
CviQI GTAC 9 cut(s) 164, 290, 493, 625, 731, 930, 1359, 2038, 2512
DdeI CTNAG 4 cut(s) 460, 561, 1451, 2264
Eam1104I CTCTTC 5 cut(s) 923, 1770, 1904, 2264, 2694
EarI CTCTTC 5 cut(s) 923, 1770, 1904, 2264, 2694
Eco130I CCWWGG 4 cut(s) 540, 911, 1966, 2041
Eco147I AGGCCT 1 cut(s) 814
Eco24I GRGCYC 1 cut(s) 2482
Eco31I GGTCTC 1 cut(s) 640
Eco32I GATATC 2 cut(s) 558, 2328
Eco47I GGWCC 2 cut(s) 584, 2683
Eco47III AGCGCT 1 cut(s) 2451
Eco57I CTGAAG 6 cut(s) 63, 99, 1365, 1590, 1941, 2147
Eco81I CCTNAGG 1 cut(s) 2264
Eco88I CYCGRG 3 cut(s) 125, 816, 1255
Eco91I GGTNACC 1 cut(s) 2495
EcoNI CCTNNNNNAGG 1 cut(s) 1074
EcoO109I RGGNCCY 1 cut(s) 2683
EcoO65I GGTNACC 1 cut(s) 2495
EcoRI GAATTC 1 cut(s) 505
EcoRII CCWGG 2 cut(s) 1204, 2175
EcoRV GATATC 2 cut(s) 558, 2328
EcoT14I CCWWGG 4 cut(s) 540, 911, 1966, 2041
EcoT22I ATGCAT 3 cut(s) 1332, 1953, 2464
EcoT38I GRGCYC 1 cut(s) 2482
ErhI CCWWGG 4 cut(s) 540, 911, 1966, 2041
FalI AAGNNNNNCTT 6 cut(s) 303, 335, 459, 491, 1832, 1864
FaqI GGGAC 5 cut(s) 284, 1093, 1190, 2288, 2669
FauNDI CATATG 1 cut(s) 2464
FbaI TGATCA 1 cut(s) 235
FblI GTMKAC 4 cut(s) 888, 1223, 1790, 2590
FokI GGATG 7 cut(s) 894, 971, 1626, 1843, 1847, 2067, 2330
FriOI GRGCYC 1 cut(s) 2482
FspBI CTAG 4 cut(s) 195, 513, 927, 2601
GlaI GCGC 1 cut(s) 2451
GsaI CCCAGC 1 cut(s) 271
GsuI CTGGAG 1 cut(s) 2159
HaeII RGCGCY 1 cut(s) 2453
HaeIII GGCC 4 cut(s) 814, 898, 1209, 1283
HhaI GCGC 1 cut(s) 2452
Hin6I GCGC 1 cut(s) 2450
HinP1I GCGC 1 cut(s) 2450
HincII GTYRAC 1 cut(s) 132
HindII GTYRAC 1 cut(s) 132
HindIII AAGCTT 3 cut(s) 17, 428, 2129
HinfI GANTC 8 cut(s) 128, 172, 405, 416, 970, 996, 1672, 1712
HphI GGTGA 4 cut(s) 877, 956, 1049, 2507
Hpy166II GTNNAC 9 cut(s) 132, 627, 713, 889, 1224, 1791, 2010, 2038, 2591
Hpy188I TCNGA 9 cut(s) 171, 415, 443, 562, 808, 850, 1345, 1759, 2127
Hpy8I GTNNAC 9 cut(s) 132, 627, 713, 889, 1224, 1791, 2010, 2038, 2591
HpyCH4III ACNGT 9 cut(s) 656, 770, 893, 1132, 1181, 1420, 1539, 1637, 2595
HpyF3I CTNAG 4 cut(s) 460, 561, 1451, 2264
HspAI GCGC 1 cut(s) 2450
Ksp22I TGATCA 1 cut(s) 235
LguI GCTCTTC 1 cut(s) 923
LmnI GCTCC 5 cut(s) 544, 1119, 1450, 1481, 2650
LweI GCATC 6 cut(s) 916, 949, 1604, 1960, 2100, 2308
MaeI CTAG 4 cut(s) 195, 513, 927, 2601
MaeIII GTNAC 8 cut(s) 206, 331, 517, 772, 1132, 1240, 1454, 2495
MboII GAAGA 9 cut(s) 411, 467, 686, 910, 979, 1757, 1852, 1891, 2281
MfeI CAATTG 1 cut(s) 1083
MflI RGATCY 2 cut(s) 1575, 2170
MhlI GDGCHC 3 cut(s) 1124, 1256, 2482
MlyI GAGTC 1 cut(s) 137
MmeI TCCRAC 1 cut(s) 421
Mph1103I ATGCAT 3 cut(s) 1332, 1953, 2464
MroXI GAANNNNTTC 2 cut(s) 20, 1926
MseI TTAA 3 cut(s) 216, 486, 1946
MslI CAYNNNNRTG 6 cut(s) 342, 376, 1873, 1946, 2463, 2487
MspA1I CMGCKG 2 cut(s) 83, 2583
MspCI CTTAAG 1 cut(s) 485
MspR9I CCNGG 2 cut(s) 1206, 2177
MunI CAATTG 1 cut(s) 1083
Mva1269I GAATGC 1 cut(s) 2572
MvaI CCWGG 2 cut(s) 1206, 2177
NdeI CATATG 1 cut(s) 2464
NlaIV GGNNCC 3 cut(s) 538, 1446, 2685
NmuCI GTSAC 4 cut(s) 206, 1132, 1454, 2495
NsiI ATGCAT 3 cut(s) 1332, 1953, 2464
NspI RCATGY 5 cut(s) 551, 1334, 1878, 2386, 2462
PaeI GCATGC 1 cut(s) 1334
PaeR7I CTCGAG 1 cut(s) 1255
PagI TCATGA 4 cut(s) 501, 552, 739, 2695
PceI AGGCCT 1 cut(s) 814
PciI ACATGT 1 cut(s) 2382
PciSI GCTCTTC 1 cut(s) 923
PctI GAATGC 1 cut(s) 2572
PdmI GAANNNNTTC 2 cut(s) 20, 1926
PfeI GAWTC 7 cut(s) 172, 405, 416, 970, 996, 1672, 1712
PflMI CCANNNNNTGG 2 cut(s) 2182, 2562
PleI GAGTC 1 cut(s) 136
PpsI GAGTC 1 cut(s) 136
PpuMI RGGWCCY 1 cut(s) 2683
PscI ACATGT 1 cut(s) 2382
PsiI TTATAA 1 cut(s) 648
Psp5II RGGWCCY 1 cut(s) 2683
Psp6I CCWGG 2 cut(s) 1204, 2175
PspEI GGTNACC 1 cut(s) 2495
PspFI CCCAGC 1 cut(s) 267
PspGI CCWGG 2 cut(s) 1204, 2175
PspN4I GGNNCC 3 cut(s) 538, 1446, 2685
PspPI GGNCC 2 cut(s) 584, 2683
PspPPI RGGWCCY 1 cut(s) 2683
PsrI GAACNNNNNNTAC 2 cut(s) 608, 640
PstI CTGCAG 1 cut(s) 791
PstNI CAGNNNCTG 1 cut(s) 2263
PsuI RGATCY 2 cut(s) 1575, 2170
PvuII CAGCTG 1 cut(s) 83
RsaI GTAC 9 cut(s) 165, 291, 494, 626, 732, 931, 1360, 2039, 2513
RsaNI GTAC 9 cut(s) 164, 290, 493, 625, 731, 930, 1359, 2038, 2512
RseI CAYNNNNRTG 6 cut(s) 342, 376, 1873, 1946, 2463, 2487
SapI GCTCTTC 1 cut(s) 923
SaqAI TTAA 3 cut(s) 216, 486, 1946
Sau96I GGNCC 2 cut(s) 584, 2683
ScaI AGTACT 1 cut(s) 2513
SchI GAGTC 1 cut(s) 137
ScrFI CCNGG 2 cut(s) 1206, 2177
SduI GDGCHC 3 cut(s) 1124, 1256, 2482
SfaNI GCATC 6 cut(s) 916, 949, 1604, 1960, 2100, 2308
SfcI CTRYAG 3 cut(s) 787, 1180, 1791
Sfr274I CTCGAG 1 cut(s) 1255
SinI GGWCC 2 cut(s) 584, 2683
SlaI CTCGAG 1 cut(s) 1255
SmiMI CAYNNNNRTG 6 cut(s) 342, 376, 1873, 1946, 2463, 2487
SmlI CTYRAG 4 cut(s) 320, 485, 1060, 1255
SmoI CTYRAG 4 cut(s) 320, 485, 1060, 1255
SpeI ACTAGT 2 cut(s) 194, 512
SphI GCATGC 1 cut(s) 1334
SseBI AGGCCT 1 cut(s) 814
SsiI CCGC 4 cut(s) 1618, 2049, 2537, 2583
SspI AATATT 2 cut(s) 758, 1486
SspMI CTAG 4 cut(s) 195, 513, 927, 2601
StuI AGGCCT 1 cut(s) 814
StyD4I CCNGG 2 cut(s) 1204, 2175
StyI CCWWGG 4 cut(s) 540, 911, 1966, 2041
TaaI ACNGT 9 cut(s) 656, 770, 893, 1132, 1181, 1420, 1539, 1637, 2595
TaqI TCGA 5 cut(s) 62, 994, 1256, 1999, 2650
TaqII GACCGA 1 cut(s) 1421
TatI WGTACW 5 cut(s) 289, 492, 624, 929, 2511
TauI GCSGC 2 cut(s) 1620, 2540
TfiI GAWTC 7 cut(s) 172, 405, 416, 970, 996, 1672, 1712
Tru1I TTAA 3 cut(s) 216, 486, 1946
Tru9I TTAA 3 cut(s) 216, 486, 1946
TscAI CASTG 4 cut(s) 211, 732, 773, 1177
TseFI GTSAC 4 cut(s) 206, 1132, 1454, 2495
Tsp45I GTSAC 4 cut(s) 206, 1132, 1454, 2495
TspDTI ATGAA 9 cut(s) 13, 29, 518, 629, 1020, 1312, 1599, 1757, 2684
TspGWI ACGGA 1 cut(s) 373
TspRI CASTG 4 cut(s) 211, 732, 773, 1177
Van91I CCANNNNNTGG 2 cut(s) 2182, 2562
Vha464I CTTAAG 1 cut(s) 485
VpaK11BI GGWCC 2 cut(s) 584, 2683
XagI CCTNNNNNAGG 1 cut(s) 1074
XapI RAATTY 8 cut(s) 90, 247, 505, 881, 1012, 1339, 1993, 2370
XceI RCATGY 5 cut(s) 551, 1334, 1878, 2386, 2462
XhoI CTCGAG 1 cut(s) 1255
XmiI GTMKAC 4 cut(s) 888, 1223, 1790, 2590
XmnI GAANNNNTTC 2 cut(s) 20, 1926
XspI CTAG 4 cut(s) 195, 513, 927, 2601
ZrmI AGTACT 1 cut(s) 2513
Zsp2I ATGCAT 3 cut(s) 1332, 1953, 2464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.