FvH4_3g20050

Protein of unknown function (DUF2921)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000420
Physical Location & Seq
Reverse (-)
13186621 .. 13189362
2742 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g20050.t1

Sequence Viewer

Length: 2742 bp
ATGACTATCTTCAGAAAGCCAAAGCTTGTCATATTCTTCTTCTGTGTTTTCAACACCCTTTCTTTGAACTCAATCTCATCCTTTGCTGTACCTAAGAAAATCTCTTATGCTGATCACTGTGCTTCATTTGTTCCCGAATCAACCCTGAAAACGGACCGCAGTTATCATCCCCATGATGAATATCATCATACAGGTTACTACTACACTGTGGGTGACACTGTCAATGGTGGTCTTAGTCGGAACTCATCATATGGTCATTATCAGCTCGGAAACTCGGTTGAATTCCGTGTCTTGAGTTTTGAAGAAACTGATGTACAAGGCTTGTTCAAGCTCCAAGCAAATGTTCTATTTCCAAAGGGCAGAACATACTCCACAACTAGGAGTAGCTTCAGGGCTCGATCAAGTCATGCAGATCCAAGATGGTTTAGACTCCACGGGTTCTGGTCAGAATCTTCAGGGAAGCTTTGCATGGTTGGATATGGTAGGTACACGAATCAAGGTACTTCACTTAATCAACCTGCTATCCTTAAGCTCTATAATCTCACCAATTCCACCACTATTACAAGTTTGATTAGTGGAACCATAGAGTCTCTGATCCCGAGTTCTGATATTGTCAAGGATCCAAGATATTTTGATCCAATCTCTATTTTGATGCTTCCTCGGATGAACTACCAGTACACTATCTCTACTGAGGACACCTCTTTCGGTGAGACTGATGATCATGATCCCTCTAGTTCTTTGAAATTAGAGAGATTTTGTTCACAACTCTCAGTAGTACATTATAATGAGTTTGATCTCAAATATTCAAGCAAATGTGTCTCGGCAAGGAATTGTACTCCGCTTGCAGTGCTTGATCAATTGCCTCGACTCATGTCGATCAAGTCAATTGATTGCTGGGAAAGTGAAAGAAGATTGAGGGTTCTGCTGGAATTCACAGACAGCAGCAACATATGGTTTCAAAGGCCTCTCAATACCAACACAACTTTGGTTGCGGAAGGCTCATGGGATGCAAAGAAAAATCAGTTACAATTTATTGCTTGCCATTTCTTAGAGGGTTCAACAGATTCTTTCAATAACTCTCATGTGGGTGATTGCTCAACAAGATTGAGCTTGAGGTTTCCTACAACATGGACAATCGAAAACACTAGTAGCGTTGTGGGGCATATTTGGAGCAACAAATCCAAGACGGAGTCTGGTTACTTCCCAAAGATCACATTCAAAACTCAACAGAGTGAGGCTGGAAGGGTTCTAATTCCTGGCACCAAATATGAGTATACAAAACTTGATCAAGTAAACAAGTTGTGCCCAAGAAAGAAATCCGCTCCTAACAAGACCATTGCTTACCCAAGTCCTATTTCTTATGAAATGAGATTTGATATGTCAGCTAGCAGTTCCAAGGGTTTAGATGCATGGGGATATTCTGAACCTGTCTCTGTCGGCAATAAGTTGTACGACGAGCTTCTTTCTTCAACGCAATATACAAACTCAGCTGCAATCAATGAAGATGAAGATGACTCTCTGGCTCCTTTGAGCTCTGTTATGATGATTTCCAGCTACAACCACAGCATCCCCACCAATATTAGCTACAGAATAAGCATTCAAATGCTAGAGAGATCTGTTCAAAATTCGAGCAATATCATAAAACACAGGTCAACGCATATGCAGATTTCTGCTGAAGGGATATATGACACAGAAGGAAGCCTGTGCATGGTAGGCTGCCGAAATGTTGGCTCAAACAATTATCACCAGACTACAGAGGATTCTGTAGATTGTGACATCCTTATCAATTTCCAGTTCCCTCCAGAAAATCAGCACAGTAATAATACAGGTTTTATCAAGGTGAGCATTGAAAGCACACGAAAAAAGTCTGATCCTCTTCATTTCGAGCGATTGGATTTGTCATCAGCTGCCAGTTACATGATTGAAGCAGAACGGTCCATCTGGAGGATGGACATGGAGATCATCTTGGTGCTTGTATCTACCACTCTTGCATGTGTATTTGTGGCATTACAACAGTTTCATGTGAAAAAACATCCTGATGTTCTTCCCGCAATTTCCATCCTGATGCTACTGACTCTAACCCTTGGCTACATGATACCTCTTATGCTAAACTTTGATGCCATGTTCACTCACAACACCAACCACAAAGATGTGTTACTGCAAAGTGGTGGATGGCTTGCAGTTCATGAGGTAATTGTAAGGGTTATAACAATGGTAGCTTTCTTGTTGCAATTCCGTCTTCTGCAGCAGACATGGTCGGCAAGATCAGCAAATGGGATGCAAAAGGAGCTTTGGGATGTGGAGAAGAAGGCTTTACCGCTGTATCTCATTGGATTTTTTGGTGCTTCGATGGACTTAAACTCTTATGCTGGTTTGGTCTTGGACGGTTTTCTGTTACCTCAAATTCTACTCAACTTGGTCCGCAAATCAAAGGAAAAGGCTCTGTCTATTCCGTTCTACATTGGAACGACTTTGGTTCGAGTGCTGCCACATGCTTATGACCTTTACAGGACTCACAGCTCTCATCAACAACTTGATGAGTCGTACATTTATGCCAGTCCTGTTGCAGATTTCTACTCTACTTCTTGGGATGTAATTATTCCTATTGGAGGTCTACTGTTTTCTCTGATCATTTTCTTGCAGCAGAAGTTTGGGGGTCGCTGCTTTCTTCCTAAAAAGTTGAGAGAGCAGGGTTTGTATGAGGAAGTCCCTTCAGTTAGCGAAGATGAGTTGCCAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
DUF2921

Protein Analysis

914

Amino Acids

103.49

Weight (kDa)

6.72

Isoelectric Point (pI)

43.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2921_N PF25333 36 - 215 2.8e-16 DUF2921 lipocalin-like domain
DUF2921_N PF25333 243 - 405 4.6e-27 DUF2921 lipocalin-like domain
DUF2921_N PF25333 431 - 632 2.4e-31 DUF2921 lipocalin-like domain
DUF2921 PF11145 643 - 894 1.1e-60 Transmembrane E3 ligase/DUF2921 transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21700
fragaria_vesca FvH4_1g10360 FvH4_1g10760 FvH4_2g30370 FvH4_3g09240 FvH4_3g20050 FvH4_3g20280 FvH4_3g20290 FvH4_3g20291 FvH4_3g20340 FvH4_3g20350 FvH4_3g20351 FvH4_3g20380 FvH4_3g20390 FvH4_5g13190 FvH4_6g20070 FvH4_6g42890
malus_domestica MD03G1227000.v1.1 MD10G1294900.v1.1 MD11G1246300.v1.1 MD11G1246400.v1.1 MD11G1246500.v1.1 MD11G1246600.v1.1
prunus_persica Prupe.4G047300_v2.0.a1 Prupe.4G181700_v2.0.a1 Prupe.4G181900_v2.0.a1 Prupe.4G182000_v2.0.a1 Prupe.4G182200_v2.0.a1
pyrus_communis pycom03g17470 pycom10g24670 pycom11g21640
rosa_chinensis RchiOBHm_Chr5g0008381 RchiOBHm_Chr5g0008391 RchiOBHm_Chr5g0033881 RchiOBHm_Chr5g0034171 RchiOBHm_Chr5g0034181 RchiOBHm_Chr5g0034191 RchiOBHm_Chr5g0034201 RchiOBHm_Chr5g0034211 RchiOBHm_Chr5g0034221 RchiOBHm_Chr5g0034231 RchiOBHm_Chr5g0034271 RchiOBHm_Chr5g0034281
rosa_laevigata RLG00000031566 RLG00000031567 RLG00000033506 RLG00000033529 RLG00000033530 RLG00000033531
rosa_multiflora Rmu_co8445509.1_g000001 Rmu_co8470615.1_g000001 Rmu_sc0008805.1_g000003 Rmu_sc0008805.1_g000004 Rmu_ssc0000128.1_g000029 Rmu_ssc0000128.1_g000031 Rmu_ssc0000128.1_g000032 Rmu_ssc0000388.1_g000062 Rmu_ssc0000388.1_g000063
rosa_roxburghii Rroxscaffold_1G00046010 Rroxscaffold_1G00046020 Rroxscaffold_1G00046030 Rroxscaffold_1G00046040 Rroxscaffold_1G00046060 Rroxscaffold_1G00046070 Rroxscaffold_1G00046080 Rroxscaffold_1G00046090
rosa_rugosa Rorug04G0435500 Rorug05G0142900 Rorug05G0144600 Rorug05G0144600 Rorug05G0144700 Rorug05G0144800
rosa_samantha Rh5AG233800 Rh5AG235900 Rh5AG236000 Rh5AG236100 Rh5AG236200 Rh5AG236300 Rh5AG236500 Rh5BG061200 Rh5BG061300 Rh5BG234300 Rh5BG236600 Rh5BG236800 Rh5CG071200 Rh5DG060000 Rh5DG060100 Rh5DG241300 Rh5DG243700 Rh5DG244000 Rh5DG244100
rosa_wichuraiana Rw3G018950 Rw5G005680 Rw5G021330 Rw5G021340 Rw5G021350 Rw5G021550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 783, 2207
Acc36I ACCTGC 1 cut(s) 526
AccB1I GGYRCC 1 cut(s) 1259
AccBSI CCGCTC 1 cut(s) 1322
AccI GTMKAC 2 cut(s) 1274, 2614
AciI CCGC 7 cut(s) 157, 839, 992, 1320, 2049, 2318, 2422
AclWI GGATC 7 cut(s) 407, 589, 614, 627, 629, 719, 1865
AcsI RAATTY 4 cut(s) 281, 929, 1624, 2403
AcuI CTGAAG 4 cut(s) 373, 438, 1695, 2697
AfaI GTAC 9 cut(s) 90, 315, 488, 502, 677, 777, 835, 1451, 2546
AfiI CCNNNNNNNGG 5 cut(s) 151, 378, 1708, 1944, 2609
AflII CTTAAG 1 cut(s) 527
AhlI ACTAGT 1 cut(s) 1145
AjnI CCWGG 1 cut(s) 1255
AjuI GAANNNNNNNTTGG 4 cut(s) 327, 359, 1339, 1371
Alw21I GWGCWC 1 cut(s) 1535
Alw26I GTCTC 4 cut(s) 594, 704, 823, 1435
AlwI GGATC 7 cut(s) 407, 589, 614, 627, 629, 719, 1865
Ama87I CYCGRG 1 cut(s) 598
AoxI GGCC 1 cut(s) 962
ApeKI GCWGC 8 cut(s) 942, 1490, 1716, 1907, 2245, 2485, 2641, 2661
ApoI RAATTY 4 cut(s) 281, 929, 1624, 2403
ArsI GACNNNNNNTTYG 2 cut(s) 686, 718
AspS9I GGNCC 3 cut(s) 154, 1935, 2419
AsuHPI GGTGA 6 cut(s) 224, 535, 719, 1100, 1736, 1852
AsuNHI GCTAGC 1 cut(s) 1385
AvaI CYCGRG 1 cut(s) 598
AvaII GGWCC 3 cut(s) 154, 1935, 2419
BaeGI GKGCMC 1 cut(s) 1307
BaeI ACNNNNGTAYC 2 cut(s) 2306, 2339
BamHI GGATCC 1 cut(s) 619
BanI GGYRCC 1 cut(s) 1259
BanII GRGCYC 2 cut(s) 397, 1535
BbsI GAAGAC 1 cut(s) 2231
Bbv12I GWGCWC 1 cut(s) 1535
BbvI GCAGC 8 cut(s) 954, 1477, 1703, 1894, 2257, 2472, 2648, 2653
BccI CCATC 6 cut(s) 414, 1942, 1946, 2066, 2166, 2344
BciT130I CCWGG 1 cut(s) 1257
BclI TGATCA 5 cut(s) 112, 718, 853, 1285, 2628
BcoDI GTCTC 4 cut(s) 594, 704, 823, 1435
BcuI ACTAGT 1 cut(s) 1145
BfaI CTAG 5 cut(s) 378, 732, 1146, 1386, 1607
BfmI CTRYAG 4 cut(s) 1585, 1752, 1764, 2243
BfrI CTTAAG 1 cut(s) 527
BfuAI ACCTGC 1 cut(s) 526
BglII AGATCT 1 cut(s) 1613
BisI GCNGC 8 cut(s) 943, 1491, 1717, 1908, 2246, 2486, 2642, 2662
BlsI GCNGC 8 cut(s) 944, 1492, 1718, 1909, 2247, 2487, 2643, 2663
Bme1390I CCNGG 1 cut(s) 1257
Bme18I GGWCC 3 cut(s) 154, 1935, 2419
BmeT110I CYCGRG 1 cut(s) 598
BmgT120I GGNCC 3 cut(s) 154, 1935, 2419
BmiI GGNNCC 4 cut(s) 580, 621, 1261, 1524
BmrFI CCNGG 1 cut(s) 1257
BmsI GCATC 7 cut(s) 642, 997, 1396, 1575, 2055, 2107, 2268
BmtI GCTAGC 1 cut(s) 1389
BoxI GACNNNNGTC 1 cut(s) 871
BpiI GAAGAC 1 cut(s) 2231
BplI GAGNNNNNCTC 2 cut(s) 683, 715
BpmI CTGGAG 2 cut(s) 1785, 1963
BpuEI CTTGAG 2 cut(s) 313, 1132
BsaBI GATNNNNATC 2 cut(s) 180, 723
BsaJI CCNNGG 5 cut(s) 433, 659, 1395, 2083, 2735
Bsc4I CCNNNNNNNGG 5 cut(s) 151, 378, 1708, 1944, 2609
Bse1I ACTGG 4 cut(s) 673, 1792, 1911, 2556
Bse3DI GCAATG 1 cut(s) 1335
Bse8I GATNNNNATC 2 cut(s) 180, 723
BseBI CCWGG 1 cut(s) 1257
BseDI CCNNGG 5 cut(s) 433, 659, 1395, 2083, 2735
BseJI GATNNNNATC 2 cut(s) 180, 723
BseLI CCNNNNNNNGG 5 cut(s) 151, 378, 1708, 1944, 2609
BseMI GCAATG 1 cut(s) 1335
BseMII CTCAG 3 cut(s) 681, 783, 1500
BseNI ACTGG 4 cut(s) 673, 1792, 1911, 2556
BseSI GKGCMC 1 cut(s) 1307
BseXI GCAGC 8 cut(s) 954, 1477, 1703, 1894, 2257, 2472, 2648, 2653
BseYI CCCAGC 1 cut(s) 894
BshFI GGCC 1 cut(s) 964
BshNI GGYRCC 1 cut(s) 1259
BsiHKAI GWGCWC 1 cut(s) 1535
BsiHKCI CYCGRG 1 cut(s) 598
BslFI GGGAC 1 cut(s) 2693
BslI CCNNNNNNNGG 5 cut(s) 151, 378, 1708, 1944, 2609
BsmAI GTCTC 4 cut(s) 594, 704, 823, 1435
BsmFI GGGAC 1 cut(s) 2693
BsmI GAATGC 1 cut(s) 1596
BsnI GGCC 1 cut(s) 964
BsoBI CYCGRG 1 cut(s) 598
Bsp1286I GDGCHC 3 cut(s) 397, 1307, 1535
Bsp1407I TGTACA 1 cut(s) 313
BspACI CCGC 7 cut(s) 157, 839, 992, 1320, 2049, 2318, 2422
BspANI GGCC 1 cut(s) 964
BspCNI CTCAG 3 cut(s) 682, 782, 1499
BspHI TCATGA 2 cut(s) 721, 2185
BspLI GGNNCC 4 cut(s) 580, 621, 1261, 1524
BspMAI CTGCAG 1 cut(s) 2247
BspMI ACCTGC 1 cut(s) 526
BspOI GCTAGC 1 cut(s) 1389
BspPI GGATC 7 cut(s) 407, 589, 614, 627, 629, 719, 1865
BspT107I GGYRCC 1 cut(s) 1259
BspTI CTTAAG 1 cut(s) 527
BsrBI CCGCTC 1 cut(s) 1322
BsrDI GCAATG 1 cut(s) 1335
BsrGI TGTACA 1 cut(s) 313
BsrI ACTGG 4 cut(s) 673, 1792, 1911, 2556
BssECI CCNNGG 5 cut(s) 433, 659, 1395, 2083, 2735
BssNAI GTATAC 1 cut(s) 1275
BssT1I CCWWGG 3 cut(s) 1395, 2083, 2735
Bst1107I GTATAC 1 cut(s) 1275
Bst2UI CCWGG 1 cut(s) 1257
Bst4CI ACNGT 8 cut(s) 119, 208, 220, 1817, 1935, 2016, 2387, 2619
Bst6I CTCTTC 1 cut(s) 1881
BstAFI CTTAAG 1 cut(s) 527
BstAUI TGTACA 1 cut(s) 313
BstC8I GCNNGC 4 cut(s) 843, 1039, 1387, 2178
BstDEI CTNAG 6 cut(s) 93, 233, 690, 769, 1048, 1486
BstDSI CCRYGG 1 cut(s) 433
BstENI CCTNNNNNAGG 1 cut(s) 2607
BstMAI GTCTC 4 cut(s) 594, 704, 823, 1435
BstMWI GCNNNNNNNGC 5 cut(s) 847, 1713, 1851, 2267, 2287
BstNI CCWGG 1 cut(s) 1257
BstNSI RCATGY 2 cut(s) 1995, 2495
BstPAI GACNNNNGTC 1 cut(s) 871
BstSCI CCNGG 1 cut(s) 1255
BstSFI CTRYAG 4 cut(s) 1585, 1752, 1764, 2243
BstSLI GKGCMC 1 cut(s) 1307
BstV1I GCAGC 8 cut(s) 954, 1477, 1703, 1894, 2257, 2472, 2648, 2653
BstV2I GAAGAC 1 cut(s) 2231
BstX2I RGATCY 3 cut(s) 412, 619, 1613
BstYI RGATCY 3 cut(s) 412, 619, 1613
BstZ17I GTATAC 1 cut(s) 1275
BsuRI GGCC 1 cut(s) 964
BtgI CCRYGG 1 cut(s) 433
BtsI GCAGTG 1 cut(s) 852
BtsIMutI CAGTG 4 cut(s) 115, 204, 216, 852
BveI ACCTGC 1 cut(s) 526
Cac8I GCNNGC 4 cut(s) 843, 1039, 1387, 2178
CciI TCATGA 2 cut(s) 721, 2185
Cfr13I GGNCC 3 cut(s) 154, 1935, 2419
CpoI CGGWCCG 1 cut(s) 154
Csp6I GTAC 9 cut(s) 89, 314, 487, 501, 676, 776, 834, 1450, 2545
CspI CGGWCCG 1 cut(s) 154
CviQI GTAC 9 cut(s) 89, 314, 487, 501, 676, 776, 834, 1450, 2545
DdeI CTNAG 6 cut(s) 93, 233, 690, 769, 1048, 1486
Eam1104I CTCTTC 1 cut(s) 1881
EarI CTCTTC 1 cut(s) 1881
Ecl136II GAGCTC 1 cut(s) 1533
Eco130I CCWWGG 3 cut(s) 1395, 2083, 2735
Eco147I AGGCCT 1 cut(s) 964
Eco24I GRGCYC 2 cut(s) 397, 1535
Eco47I GGWCC 3 cut(s) 154, 1935, 2419
Eco53kI GAGCTC 1 cut(s) 1533
Eco57I CTGAAG 4 cut(s) 373, 438, 1695, 2697
Eco88I CYCGRG 1 cut(s) 598
EcoICRI GAGCTC 1 cut(s) 1533
EcoNI CCTNNNNNAGG 1 cut(s) 2607
EcoRI GAATTC 2 cut(s) 281, 929
EcoRII CCWGG 1 cut(s) 1255
EcoT14I CCWWGG 3 cut(s) 1395, 2083, 2735
EcoT22I ATGCAT 1 cut(s) 1411
EcoT38I GRGCYC 2 cut(s) 397, 1535
ErhI CCWWGG 3 cut(s) 1395, 2083, 2735
FaqI GGGAC 1 cut(s) 2693
FauI CCCGC 1 cut(s) 2056
FauNDI CATATG 3 cut(s) 250, 950, 1659
FbaI TGATCA 5 cut(s) 112, 718, 853, 1285, 2628
FblI GTMKAC 2 cut(s) 1274, 2614
Fnu4HI GCNGC 8 cut(s) 943, 1491, 1717, 1908, 2246, 2486, 2642, 2662
FriOI GRGCYC 2 cut(s) 397, 1535
Fsp4HI GCNGC 8 cut(s) 943, 1491, 1717, 1908, 2246, 2486, 2642, 2662
FspBI CTAG 5 cut(s) 378, 732, 1146, 1386, 1607
GluI GCNGC 8 cut(s) 943, 1491, 1717, 1908, 2246, 2486, 2642, 2662
GsaI CCCAGC 1 cut(s) 898
GsuI CTGGAG 2 cut(s) 1785, 1963
HaeIII GGCC 1 cut(s) 964
HincII GTYRAC 1 cut(s) 1653
HindII GTYRAC 1 cut(s) 1653
HindIII AAGCTT 2 cut(s) 23, 461
HphI GGTGA 6 cut(s) 224, 535, 719, 1100, 1736, 1852
Hpy166II GTNNAC 8 cut(s) 489, 678, 761, 1275, 1294, 1653, 2127, 2615
Hpy188III TCNNGA 9 cut(s) 134, 292, 598, 722, 1802, 1942, 2036, 2062, 2186
Hpy8I GTNNAC 8 cut(s) 489, 678, 761, 1275, 1294, 1653, 2127, 2615
Hpy99I CGWCG 1 cut(s) 1457
HpyAV CCTTC 6 cut(s) 989, 1236, 1670, 1688, 2302, 2721
HpyCH4III ACNGT 8 cut(s) 119, 208, 220, 1817, 1935, 2016, 2387, 2619
HpyF10VI GCNNNNNNNGC 5 cut(s) 847, 1713, 1851, 2267, 2287
HpyF3I CTNAG 6 cut(s) 93, 233, 690, 769, 1048, 1486
Ksp22I TGATCA 5 cut(s) 112, 718, 853, 1285, 2628
LmnI GCTCC 5 cut(s) 336, 1170, 1327, 1528, 2287
Lsp1109I GCAGC 8 cut(s) 954, 1477, 1703, 1894, 2257, 2472, 2648, 2653
LweI GCATC 7 cut(s) 642, 997, 1396, 1575, 2055, 2107, 2268
MaeI CTAG 5 cut(s) 378, 732, 1146, 1386, 1607
MaeIII GTNAC 8 cut(s) 194, 212, 1023, 1196, 1772, 1913, 2154, 2394
MbiI CCGCTC 1 cut(s) 1322
MfeI CAATTG 2 cut(s) 857, 885
MflI RGATCY 3 cut(s) 412, 619, 1613
MhlI GDGCHC 3 cut(s) 397, 1307, 1535
MlyI GAGTC 8 cut(s) 423, 596, 861, 1199, 1508, 2068, 2506, 2549
MmeI TCCRAC 2 cut(s) 218, 454
Mph1103I ATGCAT 1 cut(s) 1411
MseI TTAA 3 cut(s) 510, 528, 2357
MslI CAYNNNNRTG 9 cut(s) 171, 783, 1086, 1601, 1967, 2037, 2063, 2148, 2496
MspA1I CMGCKG 3 cut(s) 1490, 1907, 2320
MspCI CTTAAG 1 cut(s) 527
MspR9I CCNGG 1 cut(s) 1257
MunI CAATTG 2 cut(s) 857, 885
Mva1269I GAATGC 1 cut(s) 1596
MvaI CCWGG 1 cut(s) 1257
MwoI GCNNNNNNNGC 5 cut(s) 847, 1713, 1851, 2267, 2287
NdeI CATATG 3 cut(s) 250, 950, 1659
NheI GCTAGC 1 cut(s) 1385
NlaIV GGNNCC 4 cut(s) 580, 621, 1261, 1524
NmeAIII GCCGAG 1 cut(s) 800
NmuCI GTSAC 2 cut(s) 212, 1772
NsiI ATGCAT 1 cut(s) 1411
NspI RCATGY 2 cut(s) 1995, 2495
PagI TCATGA 2 cut(s) 721, 2185
PceI AGGCCT 1 cut(s) 964
PctI GAATGC 1 cut(s) 1596
PfeI GAWTC 5 cut(s) 137, 449, 493, 1064, 1760
PflFI GACNNNGTC 3 cut(s) 218, 1189, 2254
PkrI GCNGC 8 cut(s) 944, 1492, 1718, 1909, 2247, 2487, 2643, 2663
PleI GAGTC 8 cut(s) 423, 595, 861, 1198, 1508, 2068, 2506, 2548
PpsI GAGTC 8 cut(s) 423, 595, 861, 1198, 1508, 2068, 2506, 2548
PshAI GACNNNNGTC 1 cut(s) 871
PsiI TTATAA 2 cut(s) 783, 2207
Psp124BI GAGCTC 1 cut(s) 1535
Psp6I CCWGG 1 cut(s) 1255
PspFI CCCAGC 1 cut(s) 894
PspGI CCWGG 1 cut(s) 1255
PspN4I GGNNCC 4 cut(s) 580, 621, 1261, 1524
PspPI GGNCC 3 cut(s) 154, 1935, 2419
PsrI GAACNNNNNNTAC 2 cut(s) 659, 691
PstI CTGCAG 1 cut(s) 2247
PsuI RGATCY 3 cut(s) 412, 619, 1613
PsyI GACNNNGTC 3 cut(s) 218, 1189, 2254
PvuII CAGCTG 2 cut(s) 1490, 1907
RsaI GTAC 9 cut(s) 90, 315, 488, 502, 677, 777, 835, 1451, 2546
RsaNI GTAC 9 cut(s) 89, 314, 487, 501, 676, 776, 834, 1450, 2545
RseI CAYNNNNRTG 9 cut(s) 171, 783, 1086, 1601, 1967, 2037, 2063, 2148, 2496
Rsr2I CGGWCCG 1 cut(s) 154
RsrII CGGWCCG 1 cut(s) 154
SacI GAGCTC 1 cut(s) 1535
SaqAI TTAA 3 cut(s) 510, 528, 2357
SatI GCNGC 8 cut(s) 943, 1491, 1717, 1908, 2246, 2486, 2642, 2662
Sau96I GGNCC 3 cut(s) 154, 1935, 2419
SchI GAGTC 8 cut(s) 423, 596, 861, 1199, 1508, 2068, 2506, 2549
ScrFI CCNGG 1 cut(s) 1257
SduI GDGCHC 3 cut(s) 397, 1307, 1535
SfaNI GCATC 7 cut(s) 642, 997, 1396, 1575, 2055, 2107, 2268
SfcI CTRYAG 4 cut(s) 1585, 1752, 1764, 2243
SinI GGWCC 3 cut(s) 154, 1935, 2419
SmiMI CAYNNNNRTG 9 cut(s) 171, 783, 1086, 1601, 1967, 2037, 2063, 2148, 2496
SmlI CTYRAG 3 cut(s) 292, 527, 1111
SmoI CTYRAG 3 cut(s) 292, 527, 1111
SpeI ACTAGT 1 cut(s) 1145
SseBI AGGCCT 1 cut(s) 964
SsiI CCGC 7 cut(s) 157, 839, 992, 1320, 2049, 2318, 2422
SspI AATATT 2 cut(s) 803, 1579
SspMI CTAG 5 cut(s) 378, 732, 1146, 1386, 1607
SstI GAGCTC 1 cut(s) 1535
StuI AGGCCT 1 cut(s) 964
StyD4I CCNGG 1 cut(s) 1255
StyI CCWWGG 3 cut(s) 1395, 2083, 2735
TaaI ACNGT 8 cut(s) 119, 208, 220, 1817, 1935, 2016, 2387, 2619
TaqI TCGA 8 cut(s) 397, 865, 875, 1137, 1628, 1884, 2348, 2479
TatI WGTACW 4 cut(s) 313, 675, 775, 833
TfiI GAWTC 5 cut(s) 137, 449, 493, 1064, 1760
Tru1I TTAA 3 cut(s) 510, 528, 2357
Tru9I TTAA 3 cut(s) 510, 528, 2357
TscAI CASTG 4 cut(s) 122, 211, 223, 852
TseFI GTSAC 2 cut(s) 212, 1772
TseI GCWGC 8 cut(s) 942, 1490, 1716, 1907, 2245, 2485, 2641, 2661
Tsp45I GTSAC 2 cut(s) 212, 1772
TspDTI ATGAA 9 cut(s) 114, 192, 680, 1377, 1515, 1521, 1868, 2009, 2174
TspGWI ACGGA 5 cut(s) 167, 275, 1202, 2225, 2442
TspRI CASTG 4 cut(s) 122, 211, 223, 852
Tth111I GACNNNGTC 3 cut(s) 218, 1189, 2254
Vha464I CTTAAG 1 cut(s) 527
VpaK11BI GGWCC 3 cut(s) 154, 1935, 2419
XagI CCTNNNNNAGG 1 cut(s) 2607
XapI RAATTY 4 cut(s) 281, 929, 1624, 2403
XceI RCATGY 2 cut(s) 1995, 2495
XcmI CCANNNNNNNNNTGG 2 cut(s) 982, 1945
XmiI GTMKAC 2 cut(s) 1274, 2614
XspI CTAG 5 cut(s) 378, 732, 1146, 1386, 1607
Zsp2I ATGCAT 1 cut(s) 1411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.