Rh5AG233800

Protein of unknown function (DUF2921)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000420
Physical Location & Seq
Reverse (-)
28333313 .. 28336808
3496 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG233800.1

Sequence Viewer

Length: 2781 bp
ATGACTATCTTCAGAAAGCCAAAGCTGGTCATATTCTTCTTCTGTGTTTTCACCACCTTTTCTTTGAACTCAATCTCATCCTTTGCTCTACCTAAGAAAATCGCTTATGCTGATAATTGTGCTTCAGTTGTTCCCGAGTCAACCCTGAAAGCGGACAGCAGAGATCATCGCCATGAGTATCTCCATACAGGTTACTACTACACTGGTGGTGACACTGGCAATGGTGATCTTAGTGATCCATATGGTTATTATTATCAGCTCCAAAACTCAGTTGAATTCCACGTCTGGAGTTTCGAAGAAACTGCTGTACAAGGCTTGTTCAAGCTCCGAGCAAATCTTCAATTTCCAAAGACCAGAACATATTACTACGTGGGAAACTCCACAAGTAGTAGTAGCTCCAGGTCTCGATCAATTCATGCACATCCAAGGTGGTCACTCAACTTTAGACTCCACGGGTTCTGGTCAGAATCTTCTGGGAAGCTTTGTATGGTTGGATATGGTAGTTACTCGAAGAAAGGTACTTCACTCAGTTACCCTGCTATCCTTAAGCTCTATAATCTCATCAATTCCAGTAGTATTACAAGTTTGATTACTGGAACCATAGAGTCTTTGATCCCGAGTTCTGATATTGTCAAGGATCCAAGATATTTTGATCCAACCTCTATTTTGATGCTTCCTCGGATGAACTACCAGTACACTTTGGTTTCAACTAACTCTATTGAAGACACCTCTTTTGGTGACACTGATGATCATGATCCCTCGAGTTCTTTGAAATTAGAGAACTTTTGTTCACAACTCTCAACAGTAGTACTAAAGAATGAGTTTGATCTCAAATATTCAAGCAAATGTGTTTCTGCAAGGAATTGTACTCCACTTGCTGGGGTAGATCCTTTGCCTCGACTCGTGTCGTTTAAGGATATTGATTGTAGGGAGAGAAATAGAAGATTGAGAGTTCTGGTGGAGTTCGCAGACAGCAGCAACATGTGGTTTCAAAGGCCTTTCAATCCCAACACAACTCTGGTTGCGGAAGGCTCATGGGATGCAAAGAAAAATCAACTGGAATTTGTTGCTTGTCATTTCTTAGAGGCAGCAACAGATTCTTTCAATAATTCTCATGTGGGTGACTGTTCAACAAGATTGAGCTTGAGATTTCCTGCAACATGGACAATCGGAAACACTAGTACCGTTGTGGGGCATATTTGGAGCAACAAATCCAAGACGGAGTCTGGTTACTTCGAAAAGATCAGATTCGAAACTTGTCAGCGTGAGGCCGGAAGGGTTGTAGTTCCAGGCACCAAATATGAGTATACAAAACTTGACCAAGTAAACAAGTTGTGCTCAAGAAAGAAGACTGCTGCTAATGGTCACAAAACCAATGTGTACCCAAGTCCAATTTCTTATGAGATGAGATTCCGTATGTCAGCTAGAAGATCTAAGGGATATTTAGATGCATGGGGAAGTTCTGAACCTGTCTCTGTCGGCGATAAGATCTACGAGGAGCTTCCTTCTTCAACGCAATATAGTGGTTCAGCTGCAATCGATGAAGATGAAAATTACTCTCTAGGGATAATTTCCAGCTACAACAACAGCAACCCGTCCAATATTAGCTACAGAATCAGCATTCAATTGCTGGGGCGATCTGCTGGAAAGTTGGGCAACACAACGCATATGACGGAAATGCACATCTCTGCTGAAGGGATATATGATACAGAAGGAAGCCTGTGCATGGTAGGCTGCCGAAATGTTGGCTTAAACAGTGATCACCAGACCACAGAAGATTCTGTAGATTGTGAGATCCTTGTCAATTTCCAGTTCCCTCCAGACAATCAACACAGTGAAAATAAAGGTTATATCAAGGTGAGGATTGAAAGCACACGAAAAGAGTCTGATCCTCTTCATTTCGAGCGATTGGATTTGTCATCAGTTGCTGGTTACATGAGTGAAGCAGAACGATCCATCTGGAGGATGGACATGGAGATCATCTTGGTTCTTGTATCTACCACACTCGCATGTGTATTTGTGGCATTACAACAGTTTCATGTGAAAAAACATCCTGATGTGCTTCCCTCGATTTCCATCCTGATGCTACTGATTCTAACCGTTGGCTACATGATACCTCTTATGCTAAACTTTGATGCTATGTTCACTGACAAGACCAACCGCCAATATGTGCCACTGGGAAGTGGTGGATGGCTTGCAGTTCATGAGGTAATTGTAAGGGTTATAACAATGGTAGCTTTCTTGTTGCAATTCCGTCTTCTGCAGCAGACATGGTCGGCAAGATCAGCAAATGGGAAGCAAAAGGAGCTTTGGGATGTGGAGAAGAAGGTTTTACCGCTGTATCCAATTGGGTATTTAGTTGCTTGGGCAGTGATTCCCATTTCTATGGACTTGAAATCTTATGCTGGTTTGGTCTTGGACGGTTATCTGTTGCCTCAAATTCTACTCAACGTGGTCTGCAAATCAAAGGAAAGGGCTCTGTCTGTTTCATTCTATATTGGAACAACTTTGGTTCGAGTGCTGCCACATGCTTATGACCTTTACAGGACTCACAGCTCTGTTCATCAACAACTTGATGAGTCATACATTTATGCCAGTCCTGCTGGAGATTTCTACTCTACTTCGTGGGATGTAATTATTCCTATTGGAGGTCTACTGTTTTCTCTGATCATTTTCTTGCAGCAGAAATTTGGAGGTCGTTGCTTTCTTCCTCAGAAGTTGAGAGAGCTGGGTTCATATGAGAAAGTCCCTTCCGTTATTGAAGCCGAATTGCCAACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
DUF2921

Protein Analysis

926

Amino Acids

104.48

Weight (kDa)

7.54

Isoelectric Point (pI)

39.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2921_N PF25333 36 - 220 1.5e-18 DUF2921 lipocalin-like domain
DUF2921_N PF25333 247 - 415 3.4e-30 DUF2921 lipocalin-like domain
DUF2921_N PF25333 442 - 638 2.2e-29 DUF2921 lipocalin-like domain
DUF2921 PF11145 649 - 907 6.9e-63 Transmembrane E3 ligase/DUF2921 transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21700
fragaria_vesca FvH4_1g10360 FvH4_1g10760 FvH4_2g30370 FvH4_3g09240 FvH4_3g20050 FvH4_3g20280 FvH4_3g20290 FvH4_3g20291 FvH4_3g20340 FvH4_3g20350 FvH4_3g20351 FvH4_3g20380 FvH4_3g20390 FvH4_5g13190 FvH4_6g20070 FvH4_6g42890
malus_domestica MD03G1227000.v1.1 MD10G1294900.v1.1 MD11G1246300.v1.1 MD11G1246400.v1.1 MD11G1246500.v1.1 MD11G1246600.v1.1
prunus_persica Prupe.4G047300_v2.0.a1 Prupe.4G181700_v2.0.a1 Prupe.4G181900_v2.0.a1 Prupe.4G182000_v2.0.a1 Prupe.4G182200_v2.0.a1
pyrus_communis pycom03g17470 pycom10g24670 pycom11g21640
rosa_chinensis RchiOBHm_Chr5g0008381 RchiOBHm_Chr5g0008391 RchiOBHm_Chr5g0033881 RchiOBHm_Chr5g0034171 RchiOBHm_Chr5g0034181 RchiOBHm_Chr5g0034191 RchiOBHm_Chr5g0034201 RchiOBHm_Chr5g0034211 RchiOBHm_Chr5g0034221 RchiOBHm_Chr5g0034231 RchiOBHm_Chr5g0034271 RchiOBHm_Chr5g0034281
rosa_laevigata RLG00000031566 RLG00000031567 RLG00000033506 RLG00000033529 RLG00000033530 RLG00000033531
rosa_multiflora Rmu_co8445509.1_g000001 Rmu_co8470615.1_g000001 Rmu_sc0008805.1_g000003 Rmu_sc0008805.1_g000004 Rmu_ssc0000128.1_g000029 Rmu_ssc0000128.1_g000031 Rmu_ssc0000128.1_g000032 Rmu_ssc0000388.1_g000062 Rmu_ssc0000388.1_g000063
rosa_roxburghii Rroxscaffold_1G00046010 Rroxscaffold_1G00046020 Rroxscaffold_1G00046030 Rroxscaffold_1G00046040 Rroxscaffold_1G00046060 Rroxscaffold_1G00046070 Rroxscaffold_1G00046080 Rroxscaffold_1G00046090
rosa_rugosa Rorug04G0435500 Rorug05G0142900 Rorug05G0144600 Rorug05G0144600 Rorug05G0144700 Rorug05G0144800
rosa_samantha Rh5AG233800 Rh5AG235900 Rh5AG236000 Rh5AG236100 Rh5AG236200 Rh5AG236300 Rh5AG236500 Rh5BG061200 Rh5BG061300 Rh5BG234300 Rh5BG236600 Rh5BG236800 Rh5CG071200 Rh5DG060000 Rh5DG060100 Rh5DG241300 Rh5DG243700 Rh5DG244000 Rh5DG244100
rosa_wichuraiana Rw3G018950 Rw5G005680 Rw5G021330 Rw5G021340 Rw5G021350 Rw5G021550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2225
AccB1I GGYRCC 1 cut(s) 1292
AccB7I CCANNNNNTGG 1 cut(s) 878
AccI GTMKAC 2 cut(s) 1307, 2653
AciI CCGC 4 cut(s) 152, 1025, 2161, 2336
AcsI RAATTY 4 cut(s) 275, 1061, 2439, 2687
AcuI CTGAAG 2 cut(s) 108, 1713
AfaI GTAC 7 cut(s) 309, 520, 695, 810, 868, 1183, 1382
AfiI CCNNNNNNNGG 6 cut(s) 151, 878, 1191, 1726, 1962, 2648
AflII CTTAAG 1 cut(s) 545
AflIII ACRYGT 1 cut(s) 981
AhlI ACTAGT 1 cut(s) 1178
AjiI CACGTC 1 cut(s) 283
AjnI CCWGG 2 cut(s) 398, 1288
AjuI GAANNNNNNNTTGG 2 cut(s) 1378, 1410
Alw21I GWGCWC 1 cut(s) 1340
Alw26I GTCTC 2 cut(s) 408, 1477
AlwNI CAGNNNCTG 1 cut(s) 1928
Ama87I CYCGRG 3 cut(s) 134, 616, 760
AoxI GGCC 2 cut(s) 995, 1269
ApeKI GCWGC 8 cut(s) 975, 1088, 1355, 1532, 1734, 2263, 2521, 2680
ApoI RAATTY 4 cut(s) 275, 1061, 2439, 2687
ArsI GACNNNNNNTTYG 2 cut(s) 716, 748
AsuHPI GGTGA 7 cut(s) 43, 221, 236, 749, 1133, 1754, 1870
AsuII TTCGAA 3 cut(s) 294, 1236, 1251
AvaI CYCGRG 3 cut(s) 134, 616, 760
BaeI ACNNNNGTAYC 4 cut(s) 1165, 1198, 2324, 2357
BamHI GGATCC 1 cut(s) 637
BanI GGYRCC 1 cut(s) 1292
BanII GRGCYC 1 cut(s) 2479
BauI CACGAG 1 cut(s) 902
BbsI GAAGAC 3 cut(s) 729, 1355, 2249
Bbv12I GWGCWC 1 cut(s) 1340
BbvI GCAGC 8 cut(s) 987, 1100, 1342, 1519, 1721, 2275, 2508, 2692
BccI CCATC 4 cut(s) 1960, 1964, 2084, 2184
BcgI CGANNNNNNTGC 2 cut(s) 284, 318
BciT130I CCWGG 2 cut(s) 400, 1290
BciVI GTATCC 1 cut(s) 2352
BclI TGATCA 3 cut(s) 748, 1759, 2667
BcoDI GTCTC 2 cut(s) 408, 1477
BcuI ACTAGT 1 cut(s) 1178
BfaI CTAG 3 cut(s) 1179, 1425, 1562
BfmI CTRYAG 3 cut(s) 1609, 1782, 2261
BfrI CTTAAG 1 cut(s) 545
BfuI GTATCC 1 cut(s) 2352
BglII AGATCT 2 cut(s) 1430, 1488
BisI GCNGC 8 cut(s) 976, 1089, 1356, 1533, 1735, 2264, 2522, 2681
BlsI GCNGC 8 cut(s) 977, 1090, 1357, 1534, 1736, 2265, 2523, 2682
BmcAI AGTACT 1 cut(s) 810
Bme1390I CCNGG 2 cut(s) 400, 1290
BmeT110I CYCGRG 3 cut(s) 134, 616, 760
BmgBI CACGTC 1 cut(s) 283
BmiI GGNNCC 3 cut(s) 598, 639, 1294
BmrFI CCNGG 2 cut(s) 400, 1290
BmrI ACTGGG 1 cut(s) 2186
BmsI GCATC 5 cut(s) 660, 1030, 1438, 2073, 2125
BmuI ACTGGG 1 cut(s) 2186
BoxI GACNNNNGTC 1 cut(s) 904
BpiI GAAGAC 3 cut(s) 729, 1355, 2249
BpmI CTGGAG 5 cut(s) 307, 382, 1803, 1981, 2625
Bpu14I TTCGAA 3 cut(s) 294, 1236, 1251
BpuEI CTTGAG 2 cut(s) 1165, 1324
Bsa29I ATCGAT 1 cut(s) 1539
BsaAI YACGTR 1 cut(s) 370
BsaBI GATNNNNATC 2 cut(s) 753, 2075
BsaI GGTCTC 1 cut(s) 408
BsaJI CCNNGG 3 cut(s) 425, 451, 677
Bsc4I CCNNNNNNNGG 6 cut(s) 151, 878, 1191, 1726, 1962, 2648
Bse1I ACTGG 9 cut(s) 208, 220, 570, 598, 691, 1062, 1810, 2181, 2595
Bse3DI GCAATG 1 cut(s) 226
Bse8I GATNNNNATC 2 cut(s) 753, 2075
BseBI CCWGG 2 cut(s) 400, 1290
BseCI ATCGAT 1 cut(s) 1539
BseDI CCNNGG 3 cut(s) 425, 451, 677
BseJI GATNNNNATC 2 cut(s) 753, 2075
BseLI CCNNNNNNNGG 6 cut(s) 151, 878, 1191, 1726, 1962, 2648
BseMI GCAATG 1 cut(s) 226
BseMII CTCAG 3 cut(s) 282, 541, 2726
BseNI ACTGG 9 cut(s) 208, 220, 570, 598, 691, 1062, 1810, 2181, 2595
BseRI GAGGAG 1 cut(s) 1511
BseXI GCAGC 8 cut(s) 987, 1100, 1342, 1519, 1721, 2275, 2508, 2692
BseYI CCCAGC 3 cut(s) 878, 1630, 2728
BshFI GGCC 2 cut(s) 997, 1271
BshNI GGYRCC 1 cut(s) 1292
BshVI ATCGAT 1 cut(s) 1539
BsiHKAI GWGCWC 1 cut(s) 1340
BsiHKCI CYCGRG 3 cut(s) 134, 616, 760
BsiSI CCGG 1 cut(s) 1272
BslFI GGGAC 1 cut(s) 2732
BslI CCNNNNNNNGG 6 cut(s) 151, 878, 1191, 1726, 1962, 2648
BsmAI GTCTC 2 cut(s) 408, 1477
BsmFI GGGAC 1 cut(s) 2732
BsmI GAATGC 1 cut(s) 1620
BsnI GGCC 2 cut(s) 997, 1271
Bso31I GGTCTC 1 cut(s) 408
BsoBI CYCGRG 3 cut(s) 134, 616, 760
Bsp119I TTCGAA 3 cut(s) 294, 1236, 1251
Bsp1286I GDGCHC 2 cut(s) 1340, 2479
Bsp1407I TGTACA 1 cut(s) 307
BspACI CCGC 4 cut(s) 152, 1025, 2161, 2336
BspANI GGCC 2 cut(s) 997, 1271
BspCNI CTCAG 3 cut(s) 281, 540, 2725
BspDI ATCGAT 1 cut(s) 1539
BspHI TCATGA 2 cut(s) 751, 2203
BspLI GGNNCC 3 cut(s) 598, 639, 1294
BspMAI CTGCAG 1 cut(s) 2265
BspT104I TTCGAA 3 cut(s) 294, 1236, 1251
BspT107I GGYRCC 1 cut(s) 1292
BspTI CTTAAG 1 cut(s) 545
BspTNI GGTCTC 1 cut(s) 408
BsrDI GCAATG 1 cut(s) 226
BsrGI TGTACA 1 cut(s) 307
BsrI ACTGG 9 cut(s) 208, 220, 570, 598, 691, 1062, 1810, 2181, 2595
BssECI CCNNGG 3 cut(s) 425, 451, 677
BssNAI GTATAC 1 cut(s) 1308
BssSI CACGAG 1 cut(s) 902
BssT1I CCWWGG 1 cut(s) 425
Bst1107I GTATAC 1 cut(s) 1308
Bst2BI CACGAG 1 cut(s) 902
Bst2UI CCWGG 2 cut(s) 400, 1290
Bst4CI ACNGT 9 cut(s) 805, 1127, 1186, 1757, 1835, 2034, 2101, 2423, 2658
Bst6I CTCTTC 1 cut(s) 1899
BstAFI CTTAAG 1 cut(s) 545
BstAUI TGTACA 1 cut(s) 307
BstBAI YACGTR 1 cut(s) 370
BstBI TTCGAA 3 cut(s) 294, 1236, 1251
BstC8I GCNNGC 1 cut(s) 2196
BstDEI CTNAG 7 cut(s) 93, 230, 268, 527, 1081, 1434, 2712
BstDSI CCRYGG 1 cut(s) 451
BstENI CCTNNNNNAGG 1 cut(s) 2646
BstMAI GTCTC 2 cut(s) 408, 1477
BstMWI GCNNNNNNNGC 4 cut(s) 1731, 2285, 2305, 2600
BstNI CCWGG 2 cut(s) 400, 1290
BstNSI RCATGY 3 cut(s) 985, 2013, 2531
BstPAI GACNNNNGTC 1 cut(s) 904
BstSCI CCNGG 2 cut(s) 398, 1288
BstSFI CTRYAG 3 cut(s) 1609, 1782, 2261
BstV1I GCAGC 8 cut(s) 987, 1100, 1342, 1519, 1721, 2275, 2508, 2692
BstV2I GAAGAC 3 cut(s) 729, 1355, 2249
BstX2I RGATCY 5 cut(s) 637, 886, 1430, 1488, 1794
BstXI CCANNNNNNTGG 1 cut(s) 2386
BstYI RGATCY 5 cut(s) 637, 886, 1430, 1488, 1794
BstZ17I GTATAC 1 cut(s) 1308
Bsu15I ATCGAT 1 cut(s) 1539
BsuI GTATCC 1 cut(s) 2352
BsuRI GGCC 2 cut(s) 997, 1271
BsuTUI ATCGAT 1 cut(s) 1539
BtgI CCRYGG 1 cut(s) 451
BtgZI GCGATG 1 cut(s) 152
BtrI CACGTC 1 cut(s) 283
BtsI GCAGTG 1 cut(s) 2376
BtsIMutI CAGTG 8 cut(s) 201, 213, 741, 1762, 1840, 2145, 2174, 2376
Cac8I GCNNGC 1 cut(s) 2196
CaiI CAGNNNCTG 1 cut(s) 1928
CciI TCATGA 2 cut(s) 751, 2203
ClaI ATCGAT 1 cut(s) 1539
Csp6I GTAC 7 cut(s) 308, 519, 694, 809, 867, 1182, 1381
CviQI GTAC 7 cut(s) 308, 519, 694, 809, 867, 1182, 1381
DdeI CTNAG 7 cut(s) 93, 230, 268, 527, 1081, 1434, 2712
Eam1104I CTCTTC 1 cut(s) 1899
EarI CTCTTC 1 cut(s) 1899
Eco130I CCWWGG 1 cut(s) 425
Eco147I AGGCCT 1 cut(s) 997
Eco24I GRGCYC 1 cut(s) 2479
Eco31I GGTCTC 1 cut(s) 408
Eco57I CTGAAG 2 cut(s) 108, 1713
Eco88I CYCGRG 3 cut(s) 134, 616, 760
EcoNI CCTNNNNNAGG 1 cut(s) 2646
EcoRI GAATTC 1 cut(s) 275
EcoRII CCWGG 2 cut(s) 398, 1288
EcoT14I CCWWGG 1 cut(s) 425
EcoT22I ATGCAT 1 cut(s) 1453
EcoT38I GRGCYC 1 cut(s) 2479
ErhI CCWWGG 1 cut(s) 425
FaqI GGGAC 1 cut(s) 2732
FauNDI CATATG 3 cut(s) 241, 1668, 2737
FbaI TGATCA 3 cut(s) 748, 1759, 2667
FblI GTMKAC 2 cut(s) 1307, 2653
Fnu4HI GCNGC 8 cut(s) 976, 1089, 1356, 1533, 1735, 2264, 2522, 2681
FriOI GRGCYC 1 cut(s) 2479
Fsp4HI GCNGC 8 cut(s) 976, 1089, 1356, 1533, 1735, 2264, 2522, 2681
FspBI CTAG 3 cut(s) 1179, 1425, 1562
GluI GCNGC 8 cut(s) 976, 1089, 1356, 1533, 1735, 2264, 2522, 2681
GsaI CCCAGC 3 cut(s) 882, 1634, 2732
GsuI CTGGAG 5 cut(s) 307, 382, 1803, 1981, 2625
HaeIII GGCC 2 cut(s) 997, 1271
HapII CCGG 1 cut(s) 1272
HincII GTYRAC 1 cut(s) 141
HindII GTYRAC 1 cut(s) 141
HindIII AAGCTT 1 cut(s) 479
HpaII CCGG 1 cut(s) 1272
HphI GGTGA 7 cut(s) 43, 221, 236, 749, 1133, 1754, 1870
Hpy166II GTNNAC 8 cut(s) 141, 696, 791, 1308, 1327, 1381, 2145, 2654
Hpy8I GTNNAC 8 cut(s) 141, 696, 791, 1308, 1327, 1381, 2145, 2654
HpyAV CCTTC 7 cut(s) 1022, 1269, 1515, 1688, 1706, 2320, 2760
HpyCH4III ACNGT 9 cut(s) 805, 1127, 1186, 1757, 1835, 2034, 2101, 2423, 2658
HpyCH4IV ACGT 3 cut(s) 282, 369, 2451
HpyF10VI GCNNNNNNNGC 4 cut(s) 1731, 2285, 2305, 2600
HpyF3I CTNAG 7 cut(s) 93, 230, 268, 527, 1081, 1434, 2712
HpySE526I ACGT 3 cut(s) 282, 369, 2451
Ksp22I TGATCA 3 cut(s) 748, 1759, 2667
LmnI GCTCC 6 cut(s) 264, 330, 401, 1203, 1498, 2305
Lsp1109I GCAGC 8 cut(s) 987, 1100, 1342, 1519, 1721, 2275, 2508, 2692
LweI GCATC 5 cut(s) 660, 1030, 1438, 2073, 2125
MaeI CTAG 3 cut(s) 1179, 1425, 1562
MaeII ACGT 3 cut(s) 282, 369, 2451
MfeI CAATTG 2 cut(s) 1625, 2346
MflI RGATCY 5 cut(s) 637, 886, 1430, 1488, 1794
MhlI GDGCHC 2 cut(s) 1340, 2479
MlyI GAGTC 8 cut(s) 146, 441, 614, 894, 1232, 1892, 2542, 2588
MmeI TCCRAC 2 cut(s) 472, 680
Mph1103I ATGCAT 1 cut(s) 1453
MseI TTAA 3 cut(s) 546, 912, 1751
MslI CAYNNNNRTG 7 cut(s) 171, 1119, 2008, 2055, 2081, 2384, 2532
MspA1I CMGCKG 2 cut(s) 1532, 2338
MspCI CTTAAG 1 cut(s) 545
MspI CCGG 1 cut(s) 1272
MspR9I CCNGG 2 cut(s) 400, 1290
MunI CAATTG 2 cut(s) 1625, 2346
Mva1269I GAATGC 1 cut(s) 1620
MvaI CCWGG 2 cut(s) 400, 1290
MwoI GCNNNNNNNGC 4 cut(s) 1731, 2285, 2305, 2600
NdeI CATATG 3 cut(s) 241, 1668, 2737
NlaIV GGNNCC 3 cut(s) 598, 639, 1294
NmuCI GTSAC 5 cut(s) 209, 432, 737, 1121, 1364
NsiI ATGCAT 1 cut(s) 1453
NspI RCATGY 3 cut(s) 985, 2013, 2531
NspV TTCGAA 3 cut(s) 294, 1236, 1251
PaeR7I CTCGAG 1 cut(s) 760
PagI TCATGA 2 cut(s) 751, 2203
PceI AGGCCT 1 cut(s) 997
PciI ACATGT 1 cut(s) 981
PctI GAATGC 1 cut(s) 1620
PfeI GAWTC 8 cut(s) 467, 1097, 1248, 1410, 1614, 1778, 2092, 2374
PflFI GACNNNGTC 2 cut(s) 1222, 2272
PflMI CCANNNNNTGG 1 cut(s) 878
PkrI GCNGC 8 cut(s) 977, 1090, 1357, 1534, 1736, 2265, 2523, 2682
PleI GAGTC 8 cut(s) 145, 441, 613, 894, 1231, 1891, 2542, 2587
PpsI GAGTC 8 cut(s) 145, 441, 613, 894, 1231, 1891, 2542, 2587
Ppu21I YACGTR 1 cut(s) 370
PscI ACATGT 1 cut(s) 981
PshAI GACNNNNGTC 1 cut(s) 904
PsiI TTATAA 1 cut(s) 2225
Psp6I CCWGG 2 cut(s) 398, 1288
PspFI CCCAGC 3 cut(s) 878, 1630, 2728
PspGI CCWGG 2 cut(s) 398, 1288
PspN4I GGNNCC 3 cut(s) 598, 639, 1294
PspXI VCTCGAGB 1 cut(s) 760
PsrI GAACNNNNNNTAC 2 cut(s) 677, 709
PstI CTGCAG 1 cut(s) 2265
PstNI CAGNNNCTG 1 cut(s) 1928
PsuI RGATCY 5 cut(s) 637, 886, 1430, 1488, 1794
PsyI GACNNNGTC 2 cut(s) 1222, 2272
PvuII CAGCTG 1 cut(s) 1532
RsaI GTAC 7 cut(s) 309, 520, 695, 810, 868, 1183, 1382
RsaNI GTAC 7 cut(s) 308, 519, 694, 809, 867, 1182, 1381
RseI CAYNNNNRTG 7 cut(s) 171, 1119, 2008, 2055, 2081, 2384, 2532
SaqAI TTAA 3 cut(s) 546, 912, 1751
SatI GCNGC 8 cut(s) 976, 1089, 1356, 1533, 1735, 2264, 2522, 2681
ScaI AGTACT 1 cut(s) 810
SchI GAGTC 8 cut(s) 146, 441, 614, 894, 1232, 1892, 2542, 2588
ScrFI CCNGG 2 cut(s) 400, 1290
SduI GDGCHC 2 cut(s) 1340, 2479
SfaNI GCATC 5 cut(s) 660, 1030, 1438, 2073, 2125
SfcI CTRYAG 3 cut(s) 1609, 1782, 2261
Sfr274I CTCGAG 1 cut(s) 760
SfuI TTCGAA 3 cut(s) 294, 1236, 1251
SlaI CTCGAG 1 cut(s) 760
SmiMI CAYNNNNRTG 7 cut(s) 171, 1119, 2008, 2055, 2081, 2384, 2532
SmlI CTYRAG 4 cut(s) 545, 760, 1144, 1339
SmoI CTYRAG 4 cut(s) 545, 760, 1144, 1339
SpeI ACTAGT 1 cut(s) 1178
SseBI AGGCCT 1 cut(s) 997
SsiI CCGC 4 cut(s) 152, 1025, 2161, 2336
SspI AATATT 2 cut(s) 836, 1603
SspMI CTAG 3 cut(s) 1179, 1425, 1562
StuI AGGCCT 1 cut(s) 997
StyD4I CCNGG 2 cut(s) 398, 1288
StyI CCWWGG 1 cut(s) 425
TaaI ACNGT 9 cut(s) 805, 1127, 1186, 1757, 1835, 2034, 2101, 2423, 2658
TaiI ACGT 3 cut(s) 285, 372, 2454
TatI WGTACW 4 cut(s) 307, 693, 808, 866
TfiI GAWTC 8 cut(s) 467, 1097, 1248, 1410, 1614, 1778, 2092, 2374
Tru1I TTAA 3 cut(s) 546, 912, 1751
Tru9I TTAA 3 cut(s) 546, 912, 1751
TscAI CASTG 8 cut(s) 208, 220, 748, 1762, 1840, 2152, 2181, 2376
TseFI GTSAC 5 cut(s) 209, 432, 737, 1121, 1364
TseI GCWGC 8 cut(s) 975, 1088, 1355, 1532, 1734, 2263, 2521, 2680
Tsp45I GTSAC 5 cut(s) 209, 432, 737, 1121, 1364
TspGWI ACGGA 5 cut(s) 1235, 1403, 1688, 2243, 2743
TspRI CASTG 8 cut(s) 208, 220, 748, 1762, 1840, 2152, 2181, 2376
Tth111I GACNNNGTC 2 cut(s) 1222, 2272
Van91I CCANNNNNTGG 1 cut(s) 878
Vha464I CTTAAG 1 cut(s) 545
XagI CCTNNNNNAGG 1 cut(s) 2646
XapI RAATTY 4 cut(s) 275, 1061, 2439, 2687
XceI RCATGY 3 cut(s) 985, 2013, 2531
XcmI CCANNNNNNNNNTGG 2 cut(s) 1015, 1963
XhoI CTCGAG 1 cut(s) 760
XmiI GTMKAC 2 cut(s) 1307, 2653
XspI CTAG 3 cut(s) 1179, 1425, 1562
ZrmI AGTACT 1 cut(s) 810
Zsp2I ATGCAT 1 cut(s) 1453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.