Rw5G021350

Protein of unknown function (DUF2921)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000420
Physical Location & Seq
Forward (+)
28664730 .. 28684146
19417 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G021350.1

Sequence Viewer

Length: 1140 bp
ATGCACATCTCTGCTGAAGGGATCTATGATGATACAGAAGGAAGCCTGTGCATGGTAGGATGCCGCAATCTTGGCTCAAACAGTCAGCAGGCAACAAATGATTCAATAGATTGTGAGATTCTCAATTCACATCATATCAAAGGAAGCATTGAAAGCACACGAAAGAAATCTGATCCTCTTCATTTTGAACGCTTGGATTTGTCTACAGCTTCCAAGGATGTAGGAAGGCGATCTATTTGGAGGATGGATGGGAAGATTACCTTGGTTCTTATATCCACCACACTTGTATGTGTTTTTGCTGCATTACAACTCTTCCATGTAAAAAGAAATCCTGAAAAAAGCAGGAGGATGGATGGGAAGATAACCTTGGTTCTTATATCAACCACACTTGTATGTGTTTTTGCTGCATTACAAATCTTCCATGTGAAAAGAAACCCTGAAGTCATTCCCTCTGTTTCCATCTTAATGCTTCTAATTCTAACCCTTAGCTACATGTTACCTCTCGGGATTAATATTGAAGCCATGGTCATGCATAATTTGAACCGCCAAAATGTGCACCTTGGAGGGGGTGGATGGCTTGAATTTAATGAGATAATCATAAGGGTGGCAACATTGGTAGCTTTCTTGATACAGTTCCACCTTCTGAAGCTAACTTGGTCAGCAAAATTCGGAAATGGAACCCGGAAGGATCTCCGCGTTATGGAAAAGAGGGCTCTCTTTGTGGCTTTACAAGTGTATGCTGTAGGGGCTTTAGCTGCTTTGTTTCTGCATAGCCAGAAACTGAGGAAGAGGGACGATCGCATTGAGTTGCCTCATCTAGAGAATACAGTTCTGGATGCCTTGACATCTTATGTTTGGTTCTACATTGGAACAATTGTAGCTCGAGTTCTGTCACATGCACATGCTCTTTACAGGGCTCAAAGCCCGGCCAATGACCAATTTGATGATTTCTCATACAATTATCCAGCTCCTGTGGCAGATTTATACTCCATAGCTTGGAATGCCATCATTCCTTTAGAAGGTGTACTGTTTGCTGGTATCATATACTTGCAGCAGCGGTTTGGCGGTCGTTGCATTGCTCCTGGAAAGCTGCGAGAGGTGGGTATATATAAGAAGGTCCCTACAGTTCATGAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
DUF2921

Protein Analysis

379

Amino Acids

42.58

Weight (kDa)

9.04

Isoelectric Point (pI)

46.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2921_N PF25333 1 - 66 3.1e-16 DUF2921 lipocalin-like domain
DUF2921 PF11145 115 - 363 1.5e-33 Transmembrane E3 ligase/DUF2921 transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21700
fragaria_vesca FvH4_1g10360 FvH4_1g10760 FvH4_2g30370 FvH4_3g09240 FvH4_3g20050 FvH4_3g20280 FvH4_3g20290 FvH4_3g20291 FvH4_3g20340 FvH4_3g20350 FvH4_3g20351 FvH4_3g20380 FvH4_3g20390 FvH4_5g13190 FvH4_6g20070 FvH4_6g42890
malus_domestica MD03G1227000.v1.1 MD10G1294900.v1.1 MD11G1246300.v1.1 MD11G1246400.v1.1 MD11G1246500.v1.1 MD11G1246600.v1.1
prunus_persica Prupe.4G047300_v2.0.a1 Prupe.4G181700_v2.0.a1 Prupe.4G181900_v2.0.a1 Prupe.4G182000_v2.0.a1 Prupe.4G182200_v2.0.a1
pyrus_communis pycom03g17470 pycom10g24670 pycom11g21640
rosa_chinensis RchiOBHm_Chr5g0008381 RchiOBHm_Chr5g0008391 RchiOBHm_Chr5g0033881 RchiOBHm_Chr5g0034171 RchiOBHm_Chr5g0034181 RchiOBHm_Chr5g0034191 RchiOBHm_Chr5g0034201 RchiOBHm_Chr5g0034211 RchiOBHm_Chr5g0034221 RchiOBHm_Chr5g0034231 RchiOBHm_Chr5g0034271 RchiOBHm_Chr5g0034281
rosa_laevigata RLG00000031566 RLG00000031567 RLG00000033506 RLG00000033529 RLG00000033530 RLG00000033531
rosa_multiflora Rmu_co8445509.1_g000001 Rmu_co8470615.1_g000001 Rmu_sc0008805.1_g000003 Rmu_sc0008805.1_g000004 Rmu_ssc0000128.1_g000029 Rmu_ssc0000128.1_g000031 Rmu_ssc0000128.1_g000032 Rmu_ssc0000388.1_g000062 Rmu_ssc0000388.1_g000063
rosa_roxburghii Rroxscaffold_1G00046010 Rroxscaffold_1G00046020 Rroxscaffold_1G00046030 Rroxscaffold_1G00046040 Rroxscaffold_1G00046060 Rroxscaffold_1G00046070 Rroxscaffold_1G00046080 Rroxscaffold_1G00046090
rosa_rugosa Rorug04G0435500 Rorug05G0142900 Rorug05G0144600 Rorug05G0144600 Rorug05G0144700 Rorug05G0144800
rosa_samantha Rh5AG233800 Rh5AG235900 Rh5AG236000 Rh5AG236100 Rh5AG236200 Rh5AG236300 Rh5AG236500 Rh5BG061200 Rh5BG061300 Rh5BG234300 Rh5BG236600 Rh5BG236800 Rh5CG071200 Rh5DG060000 Rh5DG060100 Rh5DG241300 Rh5DG243700 Rh5DG244000 Rh5DG244100
rosa_wichuraiana Rw3G018950 Rw5G005680 Rw5G021330 Rw5G021340 Rw5G021350 Rw5G021550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 996
AccI GTMKAC 1 cut(s) 203
AccII CGCG 1 cut(s) 696
AciI CCGC 5 cut(s) 64, 544, 694, 1057, 1065
AclWI GGATC 3 cut(s) 29, 167, 696
AcoI YGGCCR 1 cut(s) 927
AcsI RAATTY 2 cut(s) 581, 665
AcuI CTGAAG 3 cut(s) 36, 459, 665
AfaI GTAC 1 cut(s) 1026
AfiI CCNNNNNNNGG 5 cut(s) 52, 565, 700, 996, 1019
AflIII ACRYGT 1 cut(s) 492
AgsI TTSAA 6 cut(s) 105, 152, 188, 518, 541, 581
AjnI CCWGG 1 cut(s) 1081
AjuI GAANNNNNNNTTGG 4 cut(s) 245, 277, 350, 382
AluBI AGCT 9 cut(s) 209, 489, 620, 649, 755, 881, 968, 995, 1090
AluI AGCT 9 cut(s) 209, 489, 620, 649, 755, 881, 968, 995, 1090
Alw21I GWGCWC 1 cut(s) 558
Alw44I GTGCAC 1 cut(s) 554
AlwI GGATC 3 cut(s) 29, 167, 696
AlwNI CAGNNNCTG 2 cut(s) 781, 971
Ama87I CYCGRG 2 cut(s) 503, 882
AoxI GGCC 1 cut(s) 927
ApaLI GTGCAC 1 cut(s) 554
ApeKI GCWGC 6 cut(s) 299, 404, 755, 1051, 1054, 1090
ApoI RAATTY 2 cut(s) 581, 665
AseI ATTAAT 1 cut(s) 510
Asp700I GAANNNNTTC 1 cut(s) 444
AspS9I GGNCC 1 cut(s) 1117
AsuC2I CCSGG 2 cut(s) 682, 926
AvaI CYCGRG 2 cut(s) 503, 882
AvaII GGWCC 1 cut(s) 1117
BaeGI GKGCMC 1 cut(s) 558
BanII GRGCYC 2 cut(s) 715, 919
Bbv12I GWGCWC 1 cut(s) 558
BbvI GCAGC 6 cut(s) 286, 391, 742, 1063, 1066, 1077
BccI CCATC 7 cut(s) 238, 242, 343, 347, 467, 567, 1013
BciT130I CCWGG 1 cut(s) 1083
BcnI CCSGG 2 cut(s) 682, 926
BfaI CTAG 1 cut(s) 818
BfmI CTRYAG 3 cut(s) 204, 741, 1122
BisI GCNGC 7 cut(s) 64, 300, 405, 756, 1052, 1055, 1091
BlsI GCNGC 7 cut(s) 65, 301, 406, 757, 1053, 1056, 1092
Bme1390I CCNGG 3 cut(s) 682, 926, 1083
Bme18I GGWCC 1 cut(s) 1117
BmeT110I CYCGRG 2 cut(s) 503, 882
BmgT120I GGNCC 1 cut(s) 1117
BmiI GGNNCC 2 cut(s) 679, 1119
BmrFI CCNGG 3 cut(s) 682, 926, 1083
BmsI GCATC 2 cut(s) 50, 826
Bpu10I CCTNAGC 1 cut(s) 485
BpuMI CCSGG 2 cut(s) 682, 926
BsaJI CCNNGG 5 cut(s) 213, 261, 366, 522, 559
Bsc4I CCNNNNNNNGG 5 cut(s) 52, 565, 700, 996, 1019
Bse3DI GCAATG 1 cut(s) 1074
BseBI CCWGG 1 cut(s) 1083
BseDI CCNNGG 5 cut(s) 213, 261, 366, 522, 559
BseGI GGATG 8 cut(s) 65, 223, 249, 253, 354, 358, 578, 841
BseLI CCNNNNNNNGG 5 cut(s) 52, 565, 700, 996, 1019
BseMI GCAATG 1 cut(s) 1074
BseMII CTCAG 1 cut(s) 773
BseSI GKGCMC 1 cut(s) 558
BseXI GCAGC 6 cut(s) 286, 391, 742, 1063, 1066, 1077
Bsh1236I CGCG 1 cut(s) 696
Bsh1285I CGRYCG 2 cut(s) 799, 1069
BshFI GGCC 1 cut(s) 929
BsiEI CGRYCG 2 cut(s) 799, 1069
BsiHKAI GWGCWC 1 cut(s) 558
BsiHKCI CYCGRG 2 cut(s) 503, 882
BsiSI CCGG 2 cut(s) 682, 926
BslFI GGGAC 2 cut(s) 806, 1103
BslI CCNNNNNNNGG 5 cut(s) 52, 565, 700, 996, 1019
BsmFI GGGAC 2 cut(s) 806, 1103
BsmI GAATGC 1 cut(s) 1006
BsnI GGCC 1 cut(s) 929
BsoBI CYCGRG 2 cut(s) 503, 882
Bsp1286I GDGCHC 3 cut(s) 558, 715, 919
Bsp143I GATC 5 cut(s) 21, 172, 230, 688, 796
Bsp19I CCATGG 1 cut(s) 522
BspACI CCGC 5 cut(s) 64, 544, 694, 1057, 1065
BspANI GGCC 1 cut(s) 929
BspCNI CTCAG 1 cut(s) 774
BspFNI CGCG 1 cut(s) 696
BspHI TCATGA 1 cut(s) 1129
BspLI GGNNCC 2 cut(s) 679, 1119
BspPI GGATC 3 cut(s) 29, 167, 696
BsrDI GCAATG 1 cut(s) 1074
BssECI CCNNGG 5 cut(s) 213, 261, 366, 522, 559
BssMI GATC 5 cut(s) 21, 172, 230, 688, 796
BssT1I CCWWGG 5 cut(s) 213, 261, 366, 522, 559
Bst2UI CCWGG 1 cut(s) 1083
Bst4CI ACNGT 5 cut(s) 83, 633, 829, 1029, 1126
Bst6I CTCTTC 4 cut(s) 183, 317, 782, 1128
BstC8I GCNNGC 1 cut(s) 90
BstDEI CTNAG 2 cut(s) 485, 782
BstDSI CCRYGG 1 cut(s) 522
BstENI CCTNNNNNAGG 1 cut(s) 1017
BstF5I GGATG 8 cut(s) 65, 223, 249, 253, 354, 358, 578, 841
BstFNI CGCG 1 cut(s) 696
BstKTI GATC 5 cut(s) 24, 175, 233, 691, 799
BstMBI GATC 5 cut(s) 21, 172, 230, 688, 796
BstMCI CGRYCG 2 cut(s) 799, 1069
BstMWI GCNNNNNNNGC 7 cut(s) 72, 153, 746, 755, 974, 1001, 1071
BstNI CCWGG 1 cut(s) 1083
BstNSI RCATGY 3 cut(s) 496, 899, 905
BstSCI CCNGG 3 cut(s) 680, 924, 1081
BstSFI CTRYAG 3 cut(s) 204, 741, 1122
BstSLI GKGCMC 1 cut(s) 558
BstUI CGCG 1 cut(s) 696
BstV1I GCAGC 6 cut(s) 286, 391, 742, 1063, 1066, 1077
BstX2I RGATCY 2 cut(s) 21, 688
BstYI RGATCY 2 cut(s) 21, 688
BsuRI GGCC 1 cut(s) 929
BtgI CCRYGG 1 cut(s) 522
BtsCI GGATG 8 cut(s) 65, 223, 249, 253, 354, 358, 578, 841
Cac8I GCNNGC 1 cut(s) 90
CaiI CAGNNNCTG 2 cut(s) 781, 971
CciI TCATGA 1 cut(s) 1129
Cfr13I GGNCC 1 cut(s) 1117
Csp6I GTAC 1 cut(s) 1025
CspCI CAANNNNNGTGG 2 cut(s) 265, 300
CviAII CATG 9 cut(s) 52, 317, 422, 493, 523, 529, 896, 902, 1130
CviQI GTAC 1 cut(s) 1025
DdeI CTNAG 2 cut(s) 485, 782
DpnI GATC 5 cut(s) 23, 174, 232, 690, 798
DpnII GATC 5 cut(s) 21, 172, 230, 688, 796
EaeI YGGCCR 1 cut(s) 927
Eam1104I CTCTTC 4 cut(s) 183, 317, 782, 1128
EarI CTCTTC 4 cut(s) 183, 317, 782, 1128
Eco130I CCWWGG 5 cut(s) 213, 261, 366, 522, 559
Eco24I GRGCYC 2 cut(s) 715, 919
Eco47I GGWCC 1 cut(s) 1117
Eco57I CTGAAG 3 cut(s) 36, 459, 665
Eco88I CYCGRG 2 cut(s) 503, 882
EcoNI CCTNNNNNAGG 1 cut(s) 1017
EcoO109I RGGNCCY 1 cut(s) 1117
EcoRII CCWGG 1 cut(s) 1081
EcoT14I CCWWGG 5 cut(s) 213, 261, 366, 522, 559
EcoT22I ATGCAT 1 cut(s) 534
EcoT38I GRGCYC 2 cut(s) 715, 919
ErhI CCWWGG 5 cut(s) 213, 261, 366, 522, 559
FaeI CATG 9 cut(s) 55, 320, 425, 496, 526, 532, 899, 905, 1133
FalI AAGNNNNNCTT 4 cut(s) 245, 277, 350, 382
FaqI GGGAC 2 cut(s) 806, 1103
FatI CATG 9 cut(s) 51, 316, 421, 492, 522, 528, 895, 901, 1129
FblI GTMKAC 1 cut(s) 203
Fnu4HI GCNGC 7 cut(s) 64, 300, 405, 756, 1052, 1055, 1091
FokI GGATG 8 cut(s) 72, 230, 256, 260, 361, 365, 585, 848
FriOI GRGCYC 2 cut(s) 715, 919
Fsp4HI GCNGC 7 cut(s) 64, 300, 405, 756, 1052, 1055, 1091
FspBI CTAG 1 cut(s) 818
GluI GCNGC 7 cut(s) 64, 300, 405, 756, 1052, 1055, 1091
HaeIII GGCC 1 cut(s) 929
HapII CCGG 2 cut(s) 682, 926
Hin1II CATG 9 cut(s) 55, 320, 425, 496, 526, 532, 899, 905, 1133
HinfI GANTC 2 cut(s) 101, 118
HpaII CCGG 2 cut(s) 682, 926
Hpy166II GTNNAC 3 cut(s) 204, 556, 1025
Hpy188I TCNGA 3 cut(s) 172, 645, 671
Hpy188III TCNNGA 6 cut(s) 332, 505, 625, 818, 833, 1130
Hpy8I GTNNAC 3 cut(s) 204, 556, 1025
HpyAV CCTTC 7 cut(s) 11, 32, 219, 650, 679, 1013, 1108
HpyCH4III ACNGT 5 cut(s) 83, 633, 829, 1029, 1126
HpyF10VI GCNNNNNNNGC 7 cut(s) 72, 153, 746, 755, 974, 1001, 1071
HpyF3I CTNAG 2 cut(s) 485, 782
Hsp92II CATG 9 cut(s) 55, 320, 425, 496, 526, 532, 899, 905, 1133
Kzo9I GATC 5 cut(s) 21, 172, 230, 688, 796
LmnI GCTCC 2 cut(s) 973, 1084
Lsp1109I GCAGC 6 cut(s) 286, 391, 742, 1063, 1066, 1077
LweI GCATC 2 cut(s) 50, 826
MaeI CTAG 1 cut(s) 818
MaeIII GTNAC 2 cut(s) 495, 891
MalI GATC 5 cut(s) 23, 174, 232, 690, 798
MboI GATC 5 cut(s) 21, 172, 230, 688, 796
MboII GAAGA 6 cut(s) 170, 265, 304, 370, 409, 799
MfeI CAATTG 1 cut(s) 873
MflI RGATCY 2 cut(s) 21, 688
MhlI GDGCHC 3 cut(s) 558, 715, 919
MluCI AATT 8 cut(s) 124, 474, 535, 581, 665, 873, 938, 958
Mph1103I ATGCAT 1 cut(s) 534
MroXI GAANNNNTTC 1 cut(s) 444
MseI TTAA 3 cut(s) 464, 510, 585
MslI CAYNNNNRTG 6 cut(s) 286, 391, 464, 527, 602, 900
MspA1I CMGCKG 1 cut(s) 1057
MspI CCGG 2 cut(s) 682, 926
MspR9I CCNGG 3 cut(s) 682, 926, 1083
MunI CAATTG 1 cut(s) 873
Mva1269I GAATGC 1 cut(s) 1006
MvaI CCWGG 1 cut(s) 1083
MvnI CGCG 1 cut(s) 696
MwoI GCNNNNNNNGC 7 cut(s) 72, 153, 746, 755, 974, 1001, 1071
NciI CCSGG 2 cut(s) 682, 926
NcoI CCATGG 1 cut(s) 522
NdeII GATC 5 cut(s) 21, 172, 230, 688, 796
NlaIII CATG 9 cut(s) 55, 320, 425, 496, 526, 532, 899, 905, 1133
NlaIV GGNNCC 2 cut(s) 679, 1119
NmuCI GTSAC 1 cut(s) 891
NsiI ATGCAT 1 cut(s) 534
NspI RCATGY 3 cut(s) 496, 899, 905
PaeR7I CTCGAG 1 cut(s) 882
PagI TCATGA 1 cut(s) 1129
PciI ACATGT 1 cut(s) 492
PctI GAATGC 1 cut(s) 1006
PdmI GAANNNNTTC 1 cut(s) 444
PfeI GAWTC 2 cut(s) 101, 118
PflMI CCANNNNNTGG 1 cut(s) 996
PfoI TCCNGGA 1 cut(s) 1081
PkrI GCNGC 7 cut(s) 65, 301, 406, 757, 1053, 1056, 1092
Ple19I CGATCG 1 cut(s) 799
PpuMI RGGWCCY 1 cut(s) 1117
PscI ACATGT 1 cut(s) 492
PshBI ATTAAT 1 cut(s) 510
Psp5II RGGWCCY 1 cut(s) 1117
Psp6I CCWGG 1 cut(s) 1081
PspGI CCWGG 1 cut(s) 1081
PspN4I GGNNCC 2 cut(s) 679, 1119
PspPI GGNCC 1 cut(s) 1117
PspPPI RGGWCCY 1 cut(s) 1117
PspXI VCTCGAGB 1 cut(s) 882
PsrI GAACNNNNNNTAC 2 cut(s) 870, 902
PstNI CAGNNNCTG 2 cut(s) 781, 971
PsuI RGATCY 2 cut(s) 21, 688
PvuI CGATCG 1 cut(s) 799
RsaI GTAC 1 cut(s) 1026
RsaNI GTAC 1 cut(s) 1025
RseI CAYNNNNRTG 6 cut(s) 286, 391, 464, 527, 602, 900
SaqAI TTAA 3 cut(s) 464, 510, 585
SatI GCNGC 7 cut(s) 64, 300, 405, 756, 1052, 1055, 1091
Sau3AI GATC 5 cut(s) 21, 172, 230, 688, 796
Sau96I GGNCC 1 cut(s) 1117
ScrFI CCNGG 3 cut(s) 682, 926, 1083
SduI GDGCHC 3 cut(s) 558, 715, 919
SfaNI GCATC 2 cut(s) 50, 826
SfcI CTRYAG 3 cut(s) 204, 741, 1122
Sfr274I CTCGAG 1 cut(s) 882
SinI GGWCC 1 cut(s) 1117
SlaI CTCGAG 1 cut(s) 882
SmiMI CAYNNNNRTG 6 cut(s) 286, 391, 464, 527, 602, 900
SmlI CTYRAG 1 cut(s) 882
SmoI CTYRAG 1 cut(s) 882
Sse9I AATT 8 cut(s) 124, 474, 535, 581, 665, 873, 938, 958
SsiI CCGC 5 cut(s) 64, 544, 694, 1057, 1065
SspI AATATT 1 cut(s) 514
SspMI CTAG 1 cut(s) 818
StyD4I CCNGG 3 cut(s) 680, 924, 1081
StyI CCWWGG 5 cut(s) 213, 261, 366, 522, 559
TaaI ACNGT 5 cut(s) 83, 633, 829, 1029, 1126
TaqI TCGA 1 cut(s) 883
TasI AATT 8 cut(s) 124, 474, 535, 581, 665, 873, 938, 958
TatI WGTACW 1 cut(s) 1024
TauI GCSGC 1 cut(s) 66
TfiI GAWTC 2 cut(s) 101, 118
Tru1I TTAA 3 cut(s) 464, 510, 585
Tru9I TTAA 3 cut(s) 464, 510, 585
TseFI GTSAC 1 cut(s) 891
TseI GCWGC 6 cut(s) 299, 404, 755, 1051, 1054, 1090
Tsp45I GTSAC 1 cut(s) 891
TspDTI ATGAA 2 cut(s) 170, 1118
Van91I CCANNNNNTGG 1 cut(s) 996
VneI GTGCAC 1 cut(s) 554
VpaK11BI GGWCC 1 cut(s) 1117
VspI ATTAAT 1 cut(s) 510
XagI CCTNNNNNAGG 1 cut(s) 1017
XapI RAATTY 2 cut(s) 581, 665
XbaI TCTAGA 1 cut(s) 817
XceI RCATGY 3 cut(s) 496, 899, 905
XhoI CTCGAG 1 cut(s) 882
XmiI GTMKAC 1 cut(s) 203
XmnI GAANNNNTTC 1 cut(s) 444
XspI CTAG 1 cut(s) 818
Zsp2I ATGCAT 1 cut(s) 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.