Rh5BG234300

Protein of unknown function (DUF2921)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000420
Physical Location & Seq
Reverse (-)
27932045 .. 27935404
3360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG234300.1

Sequence Viewer

Length: 2796 bp
ATGACTATCTTCAGAAAGCCAAAGCTGGCCATATTCTTCTTCTGTGTTTTCACCACCCTTTCTTTGAACTCAATCTCATCCTTTGCTCTACCTAAGAAAATCGCTTATGCTGATAATTGTGCTTCAGTTGTTCCCGAGTCAACCCTGAAAGCGGACAGCAGAGATCATCGCCATGAGTATCTCCATACAGGTTACTACTACACTGGTGGTGACGCTGGCAATGGTGGTCTTAGTGGTAACTCATCATATGGTTATCATTATCAGCTCCAAAACTCAGTTGAATTCCGTATCTGGAGTTTTGAAGAAACTGATGTACAAGGCTTGTTCAAGCTCCGAGCAAATCTTCAATTTCCAAAGGACAGAACGTATTACTACGTGGGGCCCTCCACAAGTAGTAGTAGCTCCAGGTCTCGATCAATTCATGCAGATGCAAGGTGGTCACTCAACTTTAGATTCAACGGGTTCTGGTCAGAATCTTCAGGGAAGCTTTGTATGGTTGGATATGGTAGTTACTCGAAGAAAGGTACTTCACTCAGTTACCCTGCTATCCTTAAGCTCTATAATCTCTTCAATTCCACTAGTATTACAAGTTTGATTACTGGAACCATAGAGTCTTTGATCCCGAGTTCTGATATTGTCAAGGATCCAAGATATTTTGATCCAACCTCTATTTTGATGCTTCCTCGGATGAACTACCAGTACACTTTGGTTTCAACTAACTCTATTGAAGACACCTCTTTTGGTGACACTGATGATCATGATCCCTCGAGTTCTTTGAAATTGGAGAACTTTTGTTCACAACTCTCAACAGTAGTACTAAAGAATGAGTTTGATCTCAAATATTCAAGCAAATGTGTTTCTGCAAGGAATTGTACTCCACTTGCTGGGGTAGATCCTTTGCCTCGACTCGTGTCGTTTAAGGATATTGATTGTAGGGAGAGAAATAGAAGATTGAGAGTGCTGGTGGAGTTCTCAGATAGCAGCAACATGTGGTTTCAAAGGCCTTTCAATCCCAACACAACTCTGGTAGCGGAAGGCTCATGGGATGCAAAGAAAAATCAACTGGAATTTGTTGCTTGTCATTTCTTAGAGGCAGCAACAGATTCTTTCAATAATTCTCATGTGGGTGACTGTTCAACAAGATTGAGCTTGAGATTTCCTGCAACATGGACAATCGGAAACACTAGTACCGTTGTGGGGCATATTTGGAGCAACAAATCCAAGACGGAGTCTGGTTACTTCGAAAAGATCAGATTCGAAACTTGTCAGCGTGAGGCTGGAAGGGTTGTAGTTCCAGGCACCAAATACGAGTATACAAAACTTGACCAAGTAAACAAGTTGTGCCCAAGAAAGAAGACTGCTGCTAATGGTCACAAAACCAATGTGTACCCAAGTCCATTTTCTTATGAGATGAGATTCCCTATGTCAGCTAGAAGATCTAAGGGATATTTAGATGCATGGGGAAGTTCTGAACCTGTCTCTGTCGGCGATAGGTTCTACGAGGAGCTTCCTTCTTCAACGCAATATAGTGGTTCAGCTGCAATCAATGAAGATGAAAATTACTCTCTAGGGATAATTTCCAGCTACAACAACAGCAACCCGTCCAATATTAGCTACAGAATCAGCATTGAATTGCTGGGGCGATCTGCTGGAAAGTTGGGCAACACAACGCATATGACGGAAATGCTGCAAATGCATATCTCTGCTGAAGGGATATATGATACAGAAGGAAGCCTGTGCATGGTAGGCTGCCGAAATGTTCGCTTAAACAGTGATCACCAGACCACAGAAGATTCTGTAGATTGTGGGATCCTTGTCAATTTCCAGTTCCCTCCAGACAATCAACACAGTAAATATTCAAGTCATATCAAGGTGAGGATTGAAAGCACACGAGAAAAGTCTGATCGTCTTCATTTCGAGCAATTGGATTTGTCATCAGTTGGTGGTTACATGATTTCAGCAGAACGATCCATCTGGAGGATGGACATGGAGATCATCTTGGTTCTTGTATCTACCACACTCGCATGTGTATTTGTGGTATTACAACATTTTCATGTGAAAAAACATCCTGATGTGCTTCCCTCGATTTCCATCCTGATGCTATTGACTCTAACCGTTGGCTACATGATACCTCTTATGCTAAACTTTGAAGCCATGTTCACTCACAACACCAACCGCCAAGATGTGTTACTGGGAAGTGGTGGATGGCTTGCAGTTCATGAGGTAATTGTAAGGGTTATAACAATGGTAGCTTTCTTGTTGCAATTCCGTCTTCTGCAGCAGACATGGTCGGCAAGATCAGCAAATGGGAAGCAAAAGGAGCTTTGGGATGTGGAGAAGAAGGTTTTACCGCTGTATCCAATTGGGTATTTAGTTGCTTGGGCAGTGCTTCCCATTTCTGTGGACTTGAAATCTTATGCTGGTTTGGTCTTGGACGGTTATCTGTTGCCTCAAATTCTACTCAACATGGTCTGCAAATCAAAGGAAAAGGCTCTGTCTGTTTCATTCTATATTGGAACAACTTTGGTTCGAGTGCTGCCACATGCTTATGACCTTTACAGGACTCACAGCTCTGTTCATCAACAACTTGATGAGTCATACATTTATGCCAGTCCTGCTGGAGATTTCTACTCTACTTCGTGGGATGTAATTATTCCTATTGGAGGTCTACTGTTTTCTCTGATCATTTTCTTGCAGCAGAAATTTGGAGGTCGTTGCTTTCTTCCTCAGAAGTTGAGAGAGCTGGGTTCATATGAGAAAGTCCCTTCGGTTAGTGAAGCCGAATTGCCAACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
DUF2921

Protein Analysis

931

Amino Acids

104.96

Weight (kDa)

7.98

Isoelectric Point (pI)

40.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2921_N PF25333 36 - 222 4.1e-18 DUF2921 lipocalin-like domain
DUF2921_N PF25333 249 - 417 3.5e-30 DUF2921 lipocalin-like domain
DUF2921_N PF25333 444 - 642 2.4e-29 DUF2921 lipocalin-like domain
DUF2921 PF11145 654 - 912 1.7e-62 Transmembrane E3 ligase/DUF2921 transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21700
fragaria_vesca FvH4_1g10360 FvH4_1g10760 FvH4_2g30370 FvH4_3g09240 FvH4_3g20050 FvH4_3g20280 FvH4_3g20290 FvH4_3g20291 FvH4_3g20340 FvH4_3g20350 FvH4_3g20351 FvH4_3g20380 FvH4_3g20390 FvH4_5g13190 FvH4_6g20070 FvH4_6g42890
malus_domestica MD03G1227000.v1.1 MD10G1294900.v1.1 MD11G1246300.v1.1 MD11G1246400.v1.1 MD11G1246500.v1.1 MD11G1246600.v1.1
prunus_persica Prupe.4G047300_v2.0.a1 Prupe.4G181700_v2.0.a1 Prupe.4G181900_v2.0.a1 Prupe.4G182000_v2.0.a1 Prupe.4G182200_v2.0.a1
pyrus_communis pycom03g17470 pycom10g24670 pycom11g21640
rosa_chinensis RchiOBHm_Chr5g0008381 RchiOBHm_Chr5g0008391 RchiOBHm_Chr5g0033881 RchiOBHm_Chr5g0034171 RchiOBHm_Chr5g0034181 RchiOBHm_Chr5g0034191 RchiOBHm_Chr5g0034201 RchiOBHm_Chr5g0034211 RchiOBHm_Chr5g0034221 RchiOBHm_Chr5g0034231 RchiOBHm_Chr5g0034271 RchiOBHm_Chr5g0034281
rosa_laevigata RLG00000031566 RLG00000031567 RLG00000033506 RLG00000033529 RLG00000033530 RLG00000033531
rosa_multiflora Rmu_co8445509.1_g000001 Rmu_co8470615.1_g000001 Rmu_sc0008805.1_g000003 Rmu_sc0008805.1_g000004 Rmu_ssc0000128.1_g000029 Rmu_ssc0000128.1_g000031 Rmu_ssc0000128.1_g000032 Rmu_ssc0000388.1_g000062 Rmu_ssc0000388.1_g000063
rosa_roxburghii Rroxscaffold_1G00046010 Rroxscaffold_1G00046020 Rroxscaffold_1G00046030 Rroxscaffold_1G00046040 Rroxscaffold_1G00046060 Rroxscaffold_1G00046070 Rroxscaffold_1G00046080 Rroxscaffold_1G00046090
rosa_rugosa Rorug04G0435500 Rorug05G0142900 Rorug05G0144600 Rorug05G0144600 Rorug05G0144700 Rorug05G0144800
rosa_samantha Rh5AG233800 Rh5AG235900 Rh5AG236000 Rh5AG236100 Rh5AG236200 Rh5AG236300 Rh5AG236500 Rh5BG061200 Rh5BG061300 Rh5BG234300 Rh5BG236600 Rh5BG236800 Rh5CG071200 Rh5DG060000 Rh5DG060100 Rh5DG241300 Rh5DG243700 Rh5DG244000 Rh5DG244100
rosa_wichuraiana Rw3G018950 Rw5G005680 Rw5G021330 Rw5G021340 Rw5G021350 Rw5G021550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2240
AccB1I GGYRCC 1 cut(s) 1298
AccB7I CCANNNNNTGG 1 cut(s) 884
AccI GTMKAC 2 cut(s) 1313, 2668
AciI CCGC 4 cut(s) 152, 1031, 2176, 2351
AclWI GGATC 9 cut(s) 613, 638, 651, 653, 755, 887, 1804, 1817, 1962
AcoI YGGCCR 1 cut(s) 27
AcsI RAATTY 4 cut(s) 281, 1067, 2454, 2702
AcuI CTGAAG 3 cut(s) 108, 462, 1728
AfaI GTAC 7 cut(s) 315, 526, 701, 816, 874, 1189, 1388
AfiI CCNNNNNNNGG 6 cut(s) 151, 884, 1197, 1741, 1977, 2663
AflII CTTAAG 1 cut(s) 551
AflIII ACRYGT 1 cut(s) 987
AhlI ACTAGT 2 cut(s) 578, 1184
AjnI CCWGG 2 cut(s) 404, 1294
AjuI GAANNNNNNNTTGG 2 cut(s) 1384, 1416
Alw26I GTCTC 2 cut(s) 414, 1483
AlwI GGATC 9 cut(s) 613, 638, 651, 653, 755, 887, 1804, 1817, 1962
Ama87I CYCGRG 3 cut(s) 134, 622, 766
AoxI GGCC 3 cut(s) 27, 380, 1001
ApaI GGGCCC 1 cut(s) 384
ApeKI GCWGC 9 cut(s) 981, 1094, 1361, 1538, 1687, 1749, 2278, 2536, 2695
ApoI RAATTY 4 cut(s) 281, 1067, 2454, 2702
ArsI GACNNNNNNTTYG 2 cut(s) 722, 754
AspS9I GGNCC 2 cut(s) 380, 381
AsuHPI GGTGA 6 cut(s) 43, 221, 755, 1139, 1769, 1885
AsuII TTCGAA 2 cut(s) 1242, 1257
AvaI CYCGRG 3 cut(s) 134, 622, 766
BaeGI GKGCMC 2 cut(s) 384, 1346
BaeI ACNNNNGTAYC 4 cut(s) 1171, 1204, 2339, 2372
BalI TGGCCA 1 cut(s) 29
BamHI GGATCC 2 cut(s) 643, 1809
BanI GGYRCC 1 cut(s) 1298
BanII GRGCYC 1 cut(s) 384
BauI CACGAG 2 cut(s) 908, 1890
BbsI GAAGAC 4 cut(s) 735, 1361, 1901, 2264
BbvI GCAGC 9 cut(s) 993, 1106, 1348, 1525, 1674, 1736, 2290, 2523, 2707
BccI CCATC 4 cut(s) 1975, 1979, 2099, 2199
BciT130I CCWGG 2 cut(s) 406, 1296
BciVI GTATCC 1 cut(s) 2367
BclI TGATCA 3 cut(s) 754, 1774, 2682
BcoDI GTCTC 2 cut(s) 414, 1483
BcuI ACTAGT 2 cut(s) 578, 1184
BfaI CTAG 4 cut(s) 579, 1185, 1431, 1568
BfmI CTRYAG 3 cut(s) 1615, 1797, 2276
BfrI CTTAAG 1 cut(s) 551
BfuI GTATCC 1 cut(s) 2367
BglII AGATCT 1 cut(s) 1436
BisI GCNGC 9 cut(s) 982, 1095, 1362, 1539, 1688, 1750, 2279, 2537, 2696
BlsI GCNGC 9 cut(s) 983, 1096, 1363, 1540, 1689, 1751, 2280, 2538, 2697
BmcAI AGTACT 1 cut(s) 816
Bme1390I CCNGG 2 cut(s) 406, 1296
BmeT110I CYCGRG 3 cut(s) 134, 622, 766
BmgT120I GGNCC 2 cut(s) 380, 381
BmiI GGNNCC 6 cut(s) 381, 382, 604, 645, 1300, 1811
BmrFI CCNGG 2 cut(s) 406, 1296
BmrI ACTGGG 1 cut(s) 2201
BmsI GCATC 5 cut(s) 418, 666, 1036, 1444, 2088
BmuI ACTGGG 1 cut(s) 2201
BoxI GACNNNNGTC 1 cut(s) 910
BpiI GAAGAC 4 cut(s) 735, 1361, 1901, 2264
BpmI CTGGAG 5 cut(s) 313, 388, 1818, 1996, 2640
Bpu14I TTCGAA 2 cut(s) 1242, 1257
BpuEI CTTGAG 1 cut(s) 1171
BsaAI YACGTR 1 cut(s) 376
BsaBI GATNNNNATC 2 cut(s) 759, 2090
BsaI GGTCTC 1 cut(s) 414
BsaJI CCNNGG 1 cut(s) 683
Bsc4I CCNNNNNNNGG 6 cut(s) 151, 884, 1197, 1741, 1977, 2663
Bse1I ACTGG 7 cut(s) 208, 604, 697, 1068, 1825, 2196, 2610
Bse3DI GCAATG 1 cut(s) 226
Bse8I GATNNNNATC 2 cut(s) 759, 2090
BseBI CCWGG 2 cut(s) 406, 1296
BseDI CCNNGG 1 cut(s) 683
BseGI GGATG 9 cut(s) 77, 693, 1051, 1986, 2065, 2091, 2210, 2335, 2650
BseJI GATNNNNATC 2 cut(s) 759, 2090
BseLI CCNNNNNNNGG 6 cut(s) 151, 884, 1197, 1741, 1977, 2663
BseMI GCAATG 1 cut(s) 226
BseMII CTCAG 4 cut(s) 288, 547, 987, 2741
BseNI ACTGG 7 cut(s) 208, 604, 697, 1068, 1825, 2196, 2610
BseRI GAGGAG 1 cut(s) 1517
BseSI GKGCMC 2 cut(s) 384, 1346
BseXI GCAGC 9 cut(s) 993, 1106, 1348, 1525, 1674, 1736, 2290, 2523, 2707
BseYI CCCAGC 3 cut(s) 884, 1636, 2743
BshFI GGCC 3 cut(s) 29, 382, 1003
BshNI GGYRCC 1 cut(s) 1298
BsiHKCI CYCGRG 3 cut(s) 134, 622, 766
BslFI GGGAC 1 cut(s) 2747
BslI CCNNNNNNNGG 6 cut(s) 151, 884, 1197, 1741, 1977, 2663
BsmAI GTCTC 2 cut(s) 414, 1483
BsmFI GGGAC 1 cut(s) 2747
BsnI GGCC 3 cut(s) 29, 382, 1003
Bso31I GGTCTC 1 cut(s) 414
BsoBI CYCGRG 3 cut(s) 134, 622, 766
Bsp119I TTCGAA 2 cut(s) 1242, 1257
Bsp120I GGGCCC 1 cut(s) 380
Bsp1286I GDGCHC 2 cut(s) 384, 1346
Bsp1407I TGTACA 1 cut(s) 313
BspACI CCGC 4 cut(s) 152, 1031, 2176, 2351
BspANI GGCC 3 cut(s) 29, 382, 1003
BspCNI CTCAG 4 cut(s) 287, 546, 986, 2740
BspHI TCATGA 2 cut(s) 757, 2218
BspLI GGNNCC 6 cut(s) 381, 382, 604, 645, 1300, 1811
BspMAI CTGCAG 1 cut(s) 2280
BspPI GGATC 9 cut(s) 613, 638, 651, 653, 755, 887, 1804, 1817, 1962
BspT104I TTCGAA 2 cut(s) 1242, 1257
BspT107I GGYRCC 1 cut(s) 1298
BspTI CTTAAG 1 cut(s) 551
BspTNI GGTCTC 1 cut(s) 414
BsrDI GCAATG 1 cut(s) 226
BsrGI TGTACA 1 cut(s) 313
BsrI ACTGG 7 cut(s) 208, 604, 697, 1068, 1825, 2196, 2610
BssECI CCNNGG 1 cut(s) 683
BssNAI GTATAC 1 cut(s) 1314
BssSI CACGAG 2 cut(s) 908, 1890
Bst1107I GTATAC 1 cut(s) 1314
Bst2BI CACGAG 2 cut(s) 908, 1890
Bst2UI CCWGG 2 cut(s) 406, 1296
Bst4CI ACNGT 8 cut(s) 811, 1133, 1192, 1772, 1850, 2116, 2438, 2673
Bst6I CTCTTC 1 cut(s) 572
BstAFI CTTAAG 1 cut(s) 551
BstAUI TGTACA 1 cut(s) 313
BstBAI YACGTR 1 cut(s) 376
BstBI TTCGAA 2 cut(s) 1242, 1257
BstC8I GCNNGC 3 cut(s) 27, 217, 2211
BstDEI CTNAG 8 cut(s) 93, 230, 274, 533, 973, 1087, 1440, 2727
BstENI CCTNNNNNAGG 1 cut(s) 2661
BstF5I GGATG 9 cut(s) 77, 693, 1051, 1986, 2065, 2091, 2210, 2335, 2650
BstMAI GTCTC 2 cut(s) 414, 1483
BstMWI GCNNNNNNNGC 5 cut(s) 1693, 1746, 2300, 2320, 2615
BstNI CCWGG 2 cut(s) 406, 1296
BstNSI RCATGY 3 cut(s) 991, 2028, 2546
BstPAI GACNNNNGTC 1 cut(s) 910
BstSCI CCNGG 2 cut(s) 404, 1294
BstSFI CTRYAG 3 cut(s) 1615, 1797, 2276
BstSLI GKGCMC 2 cut(s) 384, 1346
BstV1I GCAGC 9 cut(s) 993, 1106, 1348, 1525, 1674, 1736, 2290, 2523, 2707
BstV2I GAAGAC 4 cut(s) 735, 1361, 1901, 2264
BstX2I RGATCY 4 cut(s) 643, 892, 1436, 1809
BstXI CCANNNNNNTGG 1 cut(s) 2401
BstYI RGATCY 4 cut(s) 643, 892, 1436, 1809
BstZ17I GTATAC 1 cut(s) 1314
BsuI GTATCC 1 cut(s) 2367
BsuRI GGCC 3 cut(s) 29, 382, 1003
BtgZI GCGATG 1 cut(s) 152
BtsCI GGATG 9 cut(s) 77, 693, 1051, 1986, 2065, 2091, 2210, 2335, 2650
BtsI GCAGTG 1 cut(s) 2391
BtsIMutI CAGTG 4 cut(s) 201, 747, 1777, 2391
Cac8I GCNNGC 3 cut(s) 27, 217, 2211
CciI TCATGA 2 cut(s) 757, 2218
Cfr13I GGNCC 2 cut(s) 380, 381
CseI GACGC 1 cut(s) 221
Csp6I GTAC 7 cut(s) 314, 525, 700, 815, 873, 1188, 1387
CviQI GTAC 7 cut(s) 314, 525, 700, 815, 873, 1188, 1387
DdeI CTNAG 8 cut(s) 93, 230, 274, 533, 973, 1087, 1440, 2727
EaeI YGGCCR 1 cut(s) 27
Eam1104I CTCTTC 1 cut(s) 572
EarI CTCTTC 1 cut(s) 572
Eco147I AGGCCT 1 cut(s) 1003
Eco24I GRGCYC 1 cut(s) 384
Eco31I GGTCTC 1 cut(s) 414
Eco57I CTGAAG 3 cut(s) 108, 462, 1728
Eco88I CYCGRG 3 cut(s) 134, 622, 766
EcoNI CCTNNNNNAGG 1 cut(s) 2661
EcoO109I RGGNCCY 2 cut(s) 380, 381
EcoRI GAATTC 1 cut(s) 281
EcoRII CCWGG 2 cut(s) 404, 1294
EcoT22I ATGCAT 2 cut(s) 1459, 1698
EcoT38I GRGCYC 1 cut(s) 384
FaqI GGGAC 1 cut(s) 2747
FauNDI CATATG 3 cut(s) 247, 1674, 2752
FbaI TGATCA 3 cut(s) 754, 1774, 2682
FblI GTMKAC 2 cut(s) 1313, 2668
Fnu4HI GCNGC 9 cut(s) 982, 1095, 1362, 1539, 1688, 1750, 2279, 2537, 2696
FokI GGATG 9 cut(s) 64, 700, 1058, 1993, 2052, 2078, 2217, 2342, 2657
FriOI GRGCYC 1 cut(s) 384
Fsp4HI GCNGC 9 cut(s) 982, 1095, 1362, 1539, 1688, 1750, 2279, 2537, 2696
FspBI CTAG 4 cut(s) 579, 1185, 1431, 1568
GluI GCNGC 9 cut(s) 982, 1095, 1362, 1539, 1688, 1750, 2279, 2537, 2696
GsaI CCCAGC 3 cut(s) 888, 1640, 2747
GsuI CTGGAG 5 cut(s) 313, 388, 1818, 1996, 2640
HaeIII GGCC 3 cut(s) 29, 382, 1003
HgaI GACGC 1 cut(s) 221
HincII GTYRAC 1 cut(s) 141
HindII GTYRAC 1 cut(s) 141
HindIII AAGCTT 1 cut(s) 485
HphI GGTGA 6 cut(s) 43, 221, 755, 1139, 1769, 1885
Hpy166II GTNNAC 9 cut(s) 141, 702, 797, 1314, 1333, 1387, 2160, 2404, 2669
Hpy8I GTNNAC 9 cut(s) 141, 702, 797, 1314, 1333, 1387, 2160, 2404, 2669
HpyAV CCTTC 7 cut(s) 1028, 1275, 1521, 1703, 1721, 2335, 2775
HpyCH4III ACNGT 8 cut(s) 811, 1133, 1192, 1772, 1850, 2116, 2438, 2673
HpyCH4IV ACGT 2 cut(s) 365, 375
HpyF10VI GCNNNNNNNGC 5 cut(s) 1693, 1746, 2300, 2320, 2615
HpyF3I CTNAG 8 cut(s) 93, 230, 274, 533, 973, 1087, 1440, 2727
HpySE526I ACGT 2 cut(s) 365, 375
Ksp22I TGATCA 3 cut(s) 754, 1774, 2682
LmnI GCTCC 6 cut(s) 270, 336, 407, 1209, 1504, 2320
Lsp1109I GCAGC 9 cut(s) 993, 1106, 1348, 1525, 1674, 1736, 2290, 2523, 2707
LweI GCATC 5 cut(s) 418, 666, 1036, 1444, 2088
MaeI CTAG 4 cut(s) 579, 1185, 1431, 1568
MaeII ACGT 2 cut(s) 365, 375
MfeI CAATTG 2 cut(s) 1922, 2361
MflI RGATCY 4 cut(s) 643, 892, 1436, 1809
MhlI GDGCHC 2 cut(s) 384, 1346
MlsI TGGCCA 1 cut(s) 29
MluNI TGGCCA 1 cut(s) 29
MlyI GAGTC 7 cut(s) 146, 620, 900, 1238, 2101, 2557, 2603
MmeI TCCRAC 2 cut(s) 478, 686
Mox20I TGGCCA 1 cut(s) 29
Mph1103I ATGCAT 2 cut(s) 1459, 1698
MscI TGGCCA 1 cut(s) 29
MseI TTAA 3 cut(s) 552, 918, 1766
MslI CAYNNNNRTG 9 cut(s) 171, 426, 1125, 2023, 2052, 2070, 2096, 2399, 2547
Msp20I TGGCCA 1 cut(s) 29
MspA1I CMGCKG 2 cut(s) 1538, 2353
MspCI CTTAAG 1 cut(s) 551
MspR9I CCNGG 2 cut(s) 406, 1296
MunI CAATTG 2 cut(s) 1922, 2361
MvaI CCWGG 2 cut(s) 406, 1296
MwoI GCNNNNNNNGC 5 cut(s) 1693, 1746, 2300, 2320, 2615
NdeI CATATG 3 cut(s) 247, 1674, 2752
NlaIV GGNNCC 6 cut(s) 381, 382, 604, 645, 1300, 1811
NmuCI GTSAC 5 cut(s) 209, 438, 743, 1127, 1370
NsiI ATGCAT 2 cut(s) 1459, 1698
NspI RCATGY 3 cut(s) 991, 2028, 2546
NspV TTCGAA 2 cut(s) 1242, 1257
PaeR7I CTCGAG 1 cut(s) 766
PagI TCATGA 2 cut(s) 757, 2218
PceI AGGCCT 1 cut(s) 1003
PciI ACATGT 1 cut(s) 987
PfeI GAWTC 7 cut(s) 453, 473, 1103, 1254, 1416, 1620, 1793
PflFI GACNNNGTC 2 cut(s) 1228, 2287
PflMI CCANNNNNTGG 1 cut(s) 884
PkrI GCNGC 9 cut(s) 983, 1096, 1363, 1540, 1689, 1751, 2280, 2538, 2697
PleI GAGTC 7 cut(s) 145, 619, 900, 1237, 2101, 2557, 2602
PpsI GAGTC 7 cut(s) 145, 619, 900, 1237, 2101, 2557, 2602
Ppu21I YACGTR 1 cut(s) 376
PscI ACATGT 1 cut(s) 987
PshAI GACNNNNGTC 1 cut(s) 910
PsiI TTATAA 1 cut(s) 2240
Psp6I CCWGG 2 cut(s) 404, 1294
PspFI CCCAGC 3 cut(s) 884, 1636, 2743
PspGI CCWGG 2 cut(s) 404, 1294
PspN4I GGNNCC 6 cut(s) 381, 382, 604, 645, 1300, 1811
PspOMI GGGCCC 1 cut(s) 380
PspPI GGNCC 2 cut(s) 380, 381
PspXI VCTCGAGB 1 cut(s) 766
PsrI GAACNNNNNNTAC 2 cut(s) 683, 715
PstI CTGCAG 1 cut(s) 2280
PsuI RGATCY 4 cut(s) 643, 892, 1436, 1809
PsyI GACNNNGTC 2 cut(s) 1228, 2287
PvuII CAGCTG 1 cut(s) 1538
RsaI GTAC 7 cut(s) 315, 526, 701, 816, 874, 1189, 1388
RsaNI GTAC 7 cut(s) 314, 525, 700, 815, 873, 1188, 1387
RseI CAYNNNNRTG 9 cut(s) 171, 426, 1125, 2023, 2052, 2070, 2096, 2399, 2547
SaqAI TTAA 3 cut(s) 552, 918, 1766
SatI GCNGC 9 cut(s) 982, 1095, 1362, 1539, 1688, 1750, 2279, 2537, 2696
Sau96I GGNCC 2 cut(s) 380, 381
ScaI AGTACT 1 cut(s) 816
SchI GAGTC 7 cut(s) 146, 620, 900, 1238, 2101, 2557, 2603
ScrFI CCNGG 2 cut(s) 406, 1296
SduI GDGCHC 2 cut(s) 384, 1346
SfaNI GCATC 5 cut(s) 418, 666, 1036, 1444, 2088
SfcI CTRYAG 3 cut(s) 1615, 1797, 2276
Sfr274I CTCGAG 1 cut(s) 766
SfuI TTCGAA 2 cut(s) 1242, 1257
SlaI CTCGAG 1 cut(s) 766
SmiMI CAYNNNNRTG 9 cut(s) 171, 426, 1125, 2023, 2052, 2070, 2096, 2399, 2547
SmlI CTYRAG 3 cut(s) 551, 766, 1150
SmoI CTYRAG 3 cut(s) 551, 766, 1150
SpeI ACTAGT 2 cut(s) 578, 1184
SseBI AGGCCT 1 cut(s) 1003
SsiI CCGC 4 cut(s) 152, 1031, 2176, 2351
SspI AATATT 3 cut(s) 842, 1609, 1856
SspMI CTAG 4 cut(s) 579, 1185, 1431, 1568
StuI AGGCCT 1 cut(s) 1003
StyD4I CCNGG 2 cut(s) 404, 1294
TaaI ACNGT 8 cut(s) 811, 1133, 1192, 1772, 1850, 2116, 2438, 2673
TaiI ACGT 2 cut(s) 368, 378
TaqI TCGA 9 cut(s) 412, 515, 767, 904, 1242, 1257, 1917, 2084, 2530
TatI WGTACW 4 cut(s) 313, 699, 814, 872
TfiI GAWTC 7 cut(s) 453, 473, 1103, 1254, 1416, 1620, 1793
Tru1I TTAA 3 cut(s) 552, 918, 1766
Tru9I TTAA 3 cut(s) 552, 918, 1766
TscAI CASTG 4 cut(s) 208, 754, 1777, 2391
TseFI GTSAC 5 cut(s) 209, 438, 743, 1127, 1370
TseI GCWGC 9 cut(s) 981, 1094, 1361, 1538, 1687, 1749, 2278, 2536, 2695
Tsp45I GTSAC 5 cut(s) 209, 438, 743, 1127, 1370
TspGWI ACGGA 4 cut(s) 275, 1241, 1694, 2258
TspRI CASTG 4 cut(s) 208, 754, 1777, 2391
Tth111I GACNNNGTC 2 cut(s) 1228, 2287
Van91I CCANNNNNTGG 1 cut(s) 884
Vha464I CTTAAG 1 cut(s) 551
XagI CCTNNNNNAGG 1 cut(s) 2661
XapI RAATTY 4 cut(s) 281, 1067, 2454, 2702
XceI RCATGY 3 cut(s) 991, 2028, 2546
XcmI CCANNNNNNNNNTGG 2 cut(s) 1021, 1978
XhoI CTCGAG 1 cut(s) 766
XmiI GTMKAC 2 cut(s) 1313, 2668
XspI CTAG 4 cut(s) 579, 1185, 1431, 1568
ZrmI AGTACT 1 cut(s) 816
Zsp2I ATGCAT 2 cut(s) 1459, 1698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.