RchiOBHm_Chr5g0008381

Protein of unknown function (DUF2921)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000420
Physical Location & Seq
Reverse (-)
5343163 .. 5346226
3064 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28936

Sequence Viewer

Length: 2841 bp
ATGGAGACCAATTTAAAATCTAGGGTGGTTTTCCCAACCACTCCTCCACAAACCCATAAATCCCTACTCACTCCCCTCCTCCTCCTCTTCTTCTTCATCAATTTCGCCCCCACAGCTTCAACCCTGAACCAAATCCCCAATCTTGATCAACTATACTCCAAACACTGCAACGACGTCGTTCCCAAATCGGAGACGCACCCCAAGTGGTTCCTCACCACCCTCTCCATCCAAGACCTCGGATTCCACAGCAGTTACTTCACCGGCGGCGATCGCCTTTTCAATCAGAGCTCCGGTGGCCGCAAAGCTCTCATCTTCCGGCCCAACTACGTAGCTCGAACCCTAGCCGATGGCATTTTCCATGTCAAAGGAAGTATGGATCTTCAATCTCTTCTGGGCTATTCGACTCGCCGGAATCTGAAGTTGGTTAAGCCCAGAATTCCGGTCCGGCGGGGCGTAGTGAGGTTTAGGCTTGATGGGTATTATTCAGAATCGTCAAAGAAGCTGTGTATGATTGGGAAAGTTATGTCCATGTATAATGCAGGTAAGTTTGAGGCTAGTGTTGTTCTTAAGCTTAATTATCCGAAATGGTCTAGTGTTTATGATAGTTTGATTACTGGGAGTTTAGAGAGCGTTGTTATTGCTGAGGGCGATGATGGTAAGAATTATTTTGATCCCGTTTTGATGTTGGGTTTGTCTGTGAAACGTAGTTATGAGTATACATTTGTTGGGAAAGATAGGCTGAGTAGTTTTCCGAGTAGTGTTGATAGAGGAGAGAGTATAGCTTTGGAGAATTTGAATGATTCAAGACGTGATTTGTGTAGGTTGCTTGGTAACCGTCTTGAGAGATATGAATTGGTGTATGGGAGTGAGTGTGGGGGTGTGAATTGCAATCCTCTTGGTGGGGATGTTGGATATGTACCTGGCTCGTTGGTTTTTTATGGGACTCGGTGTACAGAAGGGAGGAAAGTGCAGATGTTGTTGGGGTTTTCCAACTCCAGTGTAGATGAATACATAAGATTTCCATTTGTGCCTAGCACGACGTTCATTGCTGAAGGGGAATGGGATGAGAAGGGGAATCGTCTATGCGCGGTTGCATGTAGGATTCTGAATTTTACAGAATCTTTGACTAGTGCTCGTGTTGGAGACTGCTCAACTAAACTCAGCTTGAGATTTCCAGTAAAGTTGTCTTTACAAAATAGGTCTGCTGTTGTGGGGCAAATTTGGAGCGACAAAAAAGTGAGTGATCCTGGTTACTTCAGTAAAATTGGGTTCCAAGGTTTGTCGGGATGGTTAGTGAAACTCTTAGGATACAAATATGAATATTCTGTGAACGACAATCTGAGAAAAACATGTGCGGAAAAGAGGGCTGGGAGAGGCAAAGGAAAGCGATATCCTGAGGAGTATTCATCAGATCTGAAATTTGACATGAGAGTGAGCAACAGCAAAGGACAAGTTGCATGGGGTTATTCATCGCCATTATTTGTTGATGATGACCGGATTTATGGACGCCGGTTTTGGGATAAACCACAGGAAACAGAAGTACCAAGTCGTCGGAAGAAAAGCCATAGCAGTCCGATGAACATTACCTATAAGTTGAGCTTCACACGTGGTGTTCAATTTTTTCATGATGTCTTCCCATCTAAAGCAGAACTAGCTGCTGAAGGAATATATGATAGAGATTATGGAAACCTATGCATGATAGGATGTAGGCATGTACCATCCAAGGAACAAAATTCGATTAAAAAGGATATGTTAGACTGTTCTGTTAAAATTGATGTACACTTTCCTCCATTGGATACCAAGGATGGCCAGAATGTCAAGGGAACTATTGAAAGCACGAGGAATAAGTCAGACCCTCTTTATTTTGAACGTATTGACTTCTCTTCAACTTCAATTTACCATAACCAAGCTGCTGCATCCATTACGAGAATCGATTTTGAGATTGTTATGGTTTTGATTTCCAATACACTTGCATGTGCTTTTGTCGGATGGCAGCTCTATTTTGTGAAGAAAAACCCAGATGTGCTCCCATTCACTTCAATTGTCATGCTCATTGTTCTTACTCTGGGATACGTGATTCCTCTTCTGTTCAACTTTGAAGCAATGCTTGCACCAAACCAAAGCAAGCAGAGTACTTTTCTTGGGATTGGTGGATGGCTCCAGGTTAATGAAGTAATGGTCAGGGTGATAATGTTGGTAACTTTCATTTTGCAGCTGCTTCTTCTCCAACTGACCTGGTCATCGAGACAAGGTGATAATAGCCAGAAGAGCTTGATGGGTTCTGAGAGGAAGGTTCTTTATGCCACCTTACTATTGTACACAGCTGGTGCACTGATTACTTGCTCTGTCCACCAGTGGATGAGTACTCACCAGAACTCATACAGGCCATTTCATCGCAAGCTTCGGCGAGTGGCTAACCTATGGCATCTTGTTCACCCGCAACGCTCTCTCTGGGACGACCTCAAGTCTTATGCTGGATTCATCCTGGACGGTTTTCTACTCCCGCAAATATTGTTCAACTTATTCGTCAATTCAGGTGAAAAGGCTCTGTCCTGTGCCTTTTACTTTGGAACCACCATGATTCGTCTGCTGCCTCATGCTTATGATCTGTACAGAGCTCGCGCTTACACATGGTTACTTGCTTTCTCATATATTTATGCAAACCCCAGAACCGATTTTTTCTCCACTGCCTGGAACATAATCATACCCTGTTGTTGTCTGCTGCTTGCTGCCATTATTTTCTTGCAGCAAAGGTTTGGCGGCCGTTGCATCCTTCCCAAAATATCTGGGCACTCCACTGTATATGAAAAGGTCCCTGTCATCAGCAATGAGGAATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
DUF2921

Protein Analysis

946

Amino Acids

107.28

Weight (kDa)

9.28

Isoelectric Point (pI)

39.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2921_N PF25333 52 - 226 2e-18 DUF2921 lipocalin-like domain
DUF2921_N PF25333 250 - 422 8.3e-31 DUF2921 lipocalin-like domain
DUF2921_N PF25333 453 - 626 1.2e-33 DUF2921 lipocalin-like domain
DUF2921 PF11145 637 - 928 1.9e-58 Transmembrane E3 ligase/DUF2921 transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21700
fragaria_vesca FvH4_1g10360 FvH4_1g10760 FvH4_2g30370 FvH4_3g09240 FvH4_3g20050 FvH4_3g20280 FvH4_3g20290 FvH4_3g20291 FvH4_3g20340 FvH4_3g20350 FvH4_3g20351 FvH4_3g20380 FvH4_3g20390 FvH4_5g13190 FvH4_6g20070 FvH4_6g42890
malus_domestica MD03G1227000.v1.1 MD10G1294900.v1.1 MD11G1246300.v1.1 MD11G1246400.v1.1 MD11G1246500.v1.1 MD11G1246600.v1.1
prunus_persica Prupe.4G047300_v2.0.a1 Prupe.4G181700_v2.0.a1 Prupe.4G181900_v2.0.a1 Prupe.4G182000_v2.0.a1 Prupe.4G182200_v2.0.a1
pyrus_communis pycom03g17470 pycom10g24670 pycom11g21640
rosa_chinensis RchiOBHm_Chr5g0008381 RchiOBHm_Chr5g0008391 RchiOBHm_Chr5g0033881 RchiOBHm_Chr5g0034171 RchiOBHm_Chr5g0034181 RchiOBHm_Chr5g0034191 RchiOBHm_Chr5g0034201 RchiOBHm_Chr5g0034211 RchiOBHm_Chr5g0034221 RchiOBHm_Chr5g0034231 RchiOBHm_Chr5g0034271 RchiOBHm_Chr5g0034281
rosa_laevigata RLG00000031566 RLG00000031567 RLG00000033506 RLG00000033529 RLG00000033530 RLG00000033531
rosa_multiflora Rmu_co8445509.1_g000001 Rmu_co8470615.1_g000001 Rmu_sc0008805.1_g000003 Rmu_sc0008805.1_g000004 Rmu_ssc0000128.1_g000029 Rmu_ssc0000128.1_g000031 Rmu_ssc0000128.1_g000032 Rmu_ssc0000388.1_g000062 Rmu_ssc0000388.1_g000063
rosa_roxburghii Rroxscaffold_1G00046010 Rroxscaffold_1G00046020 Rroxscaffold_1G00046030 Rroxscaffold_1G00046040 Rroxscaffold_1G00046060 Rroxscaffold_1G00046070 Rroxscaffold_1G00046080 Rroxscaffold_1G00046090
rosa_rugosa Rorug04G0435500 Rorug05G0142900 Rorug05G0144600 Rorug05G0144600 Rorug05G0144700 Rorug05G0144800
rosa_samantha Rh5AG233800 Rh5AG235900 Rh5AG236000 Rh5AG236100 Rh5AG236200 Rh5AG236300 Rh5AG236500 Rh5BG061200 Rh5BG061300 Rh5BG234300 Rh5BG236600 Rh5BG236800 Rh5CG071200 Rh5DG060000 Rh5DG060100 Rh5DG241300 Rh5DG243700 Rh5DG244000 Rh5DG244100
rosa_wichuraiana Rw3G018950 Rw5G005680 Rw5G021330 Rw5G021340 Rw5G021350 Rw5G021550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 177
Acc36I ACCTGC 1 cut(s) 530
AccB7I CCANNNNNTGG 1 cut(s) 2691
AccI GTMKAC 1 cut(s) 716
AccII CGCG 2 cut(s) 1088, 2622
AciI CCGC 8 cut(s) 264, 298, 448, 1088, 1355, 2438, 2504, 2760
AclWI GGATC 3 cut(s) 384, 665, 1238
AcoI YGGCCR 3 cut(s) 295, 1807, 2761
AcsI RAATTY 6 cut(s) 435, 790, 1108, 1218, 1418, 1732
AcuI CTGAAG 4 cut(s) 437, 1071, 1240, 1680
AcvI CACGTG 1 cut(s) 1607
AcyI GRCGYC 2 cut(s) 174, 1507
AdeI CACNNNGTG 1 cut(s) 1610
AfaI GTAC 9 cut(s) 918, 952, 1542, 1716, 1779, 2134, 2318, 2365, 2612
AfiI CCNNNNNNNGG 3 cut(s) 899, 1516, 2691
AflII CTTAAG 1 cut(s) 566
AflIII ACRYGT 2 cut(s) 1349, 1604
AhdI GACNNNNNGTC 1 cut(s) 2464
AhlI ACTAGT 1 cut(s) 1127
AjiI CACGTC 1 cut(s) 809
AjnI CCWGG 6 cut(s) 919, 1246, 2160, 2234, 2484, 2690
AjuI GAANNNNNNNTTGG 2 cut(s) 404, 436
Alw21I GWGCWC 5 cut(s) 290, 1135, 2028, 2332, 2620
Alw26I GTCTC 3 cut(s) 185, 1137, 2239
Alw44I GTGCAC 1 cut(s) 2328
AlwI GGATC 3 cut(s) 384, 665, 1238
AoxI GGCC 5 cut(s) 295, 317, 1807, 2384, 2761
ApaLI GTGCAC 1 cut(s) 2328
ApoI RAATTY 6 cut(s) 435, 790, 1108, 1218, 1418, 1732
ArsI GACNNNNNNTTYG 2 cut(s) 1497, 1529
AsiSI GCGATCGC 1 cut(s) 271
AspLEI GCGC 2 cut(s) 1088, 2624
AspS9I GGNCC 3 cut(s) 318, 442, 2812
AsuHPI GGTGA 7 cut(s) 205, 250, 2197, 2264, 2360, 2426, 2549
AvaII GGWCC 2 cut(s) 442, 2812
AxyI CCTNAGG 1 cut(s) 1395
BaeGI GKGCMC 2 cut(s) 2332, 2793
BaeI ACNNNNGTAYC 2 cut(s) 1524, 1557
BalI TGGCCA 1 cut(s) 1809
BanII GRGCYC 2 cut(s) 290, 2620
BauI CACGAG 2 cut(s) 1134, 1837
BbrPI CACGTG 1 cut(s) 1607
BbsI GAAGAC 1 cut(s) 1624
Bbv12I GWGCWC 5 cut(s) 290, 1135, 2028, 2332, 2620
BbvCI CCTCAGC 1 cut(s) 642
BceAI ACGGC 1 cut(s) 2748
BcgI CGANNNNNNTGC 2 cut(s) 157, 191
BciT130I CCWGG 6 cut(s) 921, 1248, 2162, 2236, 2486, 2692
BciVI GTATCC 3 cut(s) 1301, 1789, 2063
BclI TGATCA 1 cut(s) 145
BcoDI GTCTC 3 cut(s) 185, 1137, 2239
BcuI ACTAGT 1 cut(s) 1127
BfaI CTAG 7 cut(s) 21, 341, 555, 591, 1032, 1128, 1652
BfrI CTTAAG 1 cut(s) 566
BfuAI ACCTGC 1 cut(s) 530
BfuI GTATCC 3 cut(s) 1301, 1789, 2063
BglII AGATCT 1 cut(s) 1411
BmcAI AGTACT 2 cut(s) 2134, 2365
Bme1390I CCNGG 6 cut(s) 921, 1248, 2162, 2236, 2486, 2692
Bme18I GGWCC 2 cut(s) 442, 2812
BmeRI GACNNNNNGTC 1 cut(s) 2464
BmgBI CACGTC 1 cut(s) 809
BmgT120I GGNCC 3 cut(s) 318, 442, 2812
BmiI GGNNCC 5 cut(s) 209, 1271, 2159, 2572, 2814
BmrFI CCNGG 6 cut(s) 921, 1248, 2162, 2236, 2486, 2692
BmrI ACTGGG 1 cut(s) 624
BmsI GCATC 3 cut(s) 1925, 2434, 2778
BmuI ACTGGG 1 cut(s) 624
BpiI GAAGAC 1 cut(s) 1624
BpmI CTGGAG 2 cut(s) 979, 2144
Bpu10I CCTNAGC 1 cut(s) 642
BpuEI CTTGAG 3 cut(s) 860, 1186, 2447
Bsa29I ATCGAT 1 cut(s) 1932
BsaAI YACGTR 3 cut(s) 328, 1607, 2074
BsaHI GRCGYC 2 cut(s) 174, 1507
BsaJI CCNNGG 4 cut(s) 235, 1273, 1722, 1800
BsaWI WCCGGW 3 cut(s) 290, 439, 1494
BsaXI ACNNNNNCTCC 4 cut(s) 28, 58, 856, 886
Bsc4I CCNNNNNNNGG 3 cut(s) 899, 1516, 2691
Bse118I RCCGGY 2 cut(s) 260, 1509
Bse1I ACTGG 4 cut(s) 619, 996, 1175, 2353
Bse21I CCTNAGG 1 cut(s) 1395
Bse3DI GCAATG 3 cut(s) 1044, 2109, 2833
BseBI CCWGG 6 cut(s) 921, 1248, 2162, 2236, 2486, 2692
BseCI ATCGAT 1 cut(s) 1932
BseDI CCNNGG 4 cut(s) 235, 1273, 1722, 1800
BseLI CCNNNNNNNGG 3 cut(s) 899, 1516, 2691
BseMI GCAATG 3 cut(s) 1044, 2109, 2833
BseMII CTCAG 6 cut(s) 633, 731, 1174, 1331, 1386, 2274
BseNI ACTGG 4 cut(s) 619, 996, 1175, 2353
BseRI GAGGAG 6 cut(s) 33, 68, 71, 74, 783, 1412
BseSI GKGCMC 2 cut(s) 2332, 2793
BseX3I CGGCCG 1 cut(s) 2761
BseYI CCCAGC 1 cut(s) 1367
BsgI GTGCAG 1 cut(s) 989
Bsh1236I CGCG 2 cut(s) 1088, 2622
Bsh1285I CGRYCG 2 cut(s) 271, 2764
BshFI GGCC 5 cut(s) 297, 319, 1809, 2386, 2763
BshVI ATCGAT 1 cut(s) 1932
BsiEI CGRYCG 2 cut(s) 271, 2764
BsiHKAI GWGCWC 5 cut(s) 290, 1135, 2028, 2332, 2620
BsiSI CCGG 8 cut(s) 261, 291, 316, 409, 440, 445, 1495, 1510
BslFI GGGAC 3 cut(s) 955, 2468, 2798
BslI CCNNNNNNNGG 3 cut(s) 899, 1516, 2691
BsmAI GTCTC 3 cut(s) 185, 1137, 2239
BsmBI CGTCTC 1 cut(s) 185
BsmFI GGGAC 3 cut(s) 955, 2468, 2798
BsnI GGCC 5 cut(s) 297, 319, 1809, 2386, 2763
Bsp1286I GDGCHC 6 cut(s) 290, 1135, 2028, 2332, 2620, 2793
Bsp1407I TGTACA 4 cut(s) 950, 1777, 2316, 2610
Bsp143I GATC 7 cut(s) 145, 268, 376, 670, 1243, 1411, 2605
BspACI CCGC 8 cut(s) 264, 298, 448, 1088, 1355, 2438, 2504, 2760
BspANI GGCC 5 cut(s) 297, 319, 1809, 2386, 2763
BspCNI CTCAG 6 cut(s) 634, 732, 1173, 1332, 1387, 2275
BspDI ATCGAT 1 cut(s) 1932
BspFNI CGCG 2 cut(s) 1088, 2622
BspHI TCATGA 1 cut(s) 1624
BspLI GGNNCC 5 cut(s) 209, 1271, 2159, 2572, 2814
BspMI ACCTGC 1 cut(s) 530
BspPI GGATC 3 cut(s) 384, 665, 1238
BspQI GCTCTTC 1 cut(s) 2261
BspTI CTTAAG 1 cut(s) 566
BsrDI GCAATG 3 cut(s) 1044, 2109, 2833
BsrFI RCCGGY 2 cut(s) 260, 1509
BsrGI TGTACA 4 cut(s) 950, 1777, 2316, 2610
BsrI ACTGG 4 cut(s) 619, 996, 1175, 2353
BssAI RCCGGY 2 cut(s) 260, 1509
BssECI CCNNGG 4 cut(s) 235, 1273, 1722, 1800
BssMI GATC 7 cut(s) 145, 268, 376, 670, 1243, 1411, 2605
BssNAI GTATAC 1 cut(s) 717
BssNI GRCGYC 2 cut(s) 174, 1507
BssSI CACGAG 2 cut(s) 1134, 1837
BssT1I CCWWGG 3 cut(s) 1273, 1722, 1800
Bst1107I GTATAC 1 cut(s) 717
Bst2BI CACGAG 2 cut(s) 1134, 1837
Bst2UI CCWGG 6 cut(s) 921, 1248, 2162, 2236, 2486, 2692
Bst4CI ACNGT 4 cut(s) 836, 1760, 2492, 2800
Bst6I CTCTTC 5 cut(s) 92, 393, 1888, 2088, 2261
BstACI GRCGYC 2 cut(s) 174, 1507
BstAFI CTTAAG 1 cut(s) 566
BstAPI GCANNNNNTGC 1 cut(s) 2108
BstAUI TGTACA 4 cut(s) 950, 1777, 2316, 2610
BstBAI YACGTR 3 cut(s) 328, 1607, 2074
BstC8I GCNNGC 5 cut(s) 2109, 2126, 2399, 2620, 2727
BstDEI CTNAG 7 cut(s) 642, 740, 1160, 1303, 1340, 1395, 2283
BstEII GGTNACC 1 cut(s) 830
BstFNI CGCG 2 cut(s) 1088, 2622
BstHHI GCGC 2 cut(s) 1088, 2624
BstKTI GATC 7 cut(s) 148, 271, 379, 673, 1246, 1414, 2608
BstMAI GTCTC 3 cut(s) 185, 1137, 2239
BstMBI GATC 7 cut(s) 145, 268, 376, 670, 1243, 1411, 2605
BstMCI CGRYCG 2 cut(s) 271, 2764
BstMWI GCNNNNNNNGC 7 cut(s) 113, 270, 294, 1652, 2108, 2268, 2766
BstNI CCWGG 6 cut(s) 921, 1248, 2162, 2236, 2486, 2692
BstNSI RCATGY 4 cut(s) 1098, 1353, 1715, 1977
BstPI GGTNACC 1 cut(s) 830
BstSCI CCNGG 6 cut(s) 919, 1246, 2160, 2234, 2484, 2690
BstSLI GKGCMC 2 cut(s) 2332, 2793
BstSNI TACGTA 1 cut(s) 328
BstUI CGCG 2 cut(s) 1088, 2622
BstV2I GAAGAC 1 cut(s) 1624
BstX2I RGATCY 2 cut(s) 376, 1411
BstYI RGATCY 2 cut(s) 376, 1411
BstZ17I GTATAC 1 cut(s) 717
BstZI CGGCCG 1 cut(s) 2761
Bsu15I ATCGAT 1 cut(s) 1932
Bsu36I CCTNAGG 1 cut(s) 1395
BsuI GTATCC 3 cut(s) 1301, 1789, 2063
BsuRI GGCC 5 cut(s) 297, 319, 1809, 2386, 2763
BsuTUI ATCGAT 1 cut(s) 1932
BtgZI GCGATG 3 cut(s) 663, 1455, 2378
BtrI CACGTC 1 cut(s) 809
BtsI GCAGTG 2 cut(s) 163, 2685
BtsIMutI CAGTG 6 cut(s) 163, 1003, 2330, 2360, 2685, 2796
BveI ACCTGC 1 cut(s) 530
Cac8I GCNNGC 5 cut(s) 2109, 2126, 2399, 2620, 2727
CciI TCATGA 1 cut(s) 1624
CfoI GCGC 2 cut(s) 1088, 2624
Cfr10I RCCGGY 2 cut(s) 260, 1509
Cfr13I GGNCC 3 cut(s) 318, 442, 2812
ClaI ATCGAT 1 cut(s) 1932
CpoI CGGWCCG 1 cut(s) 442
CseI GACGC 2 cut(s) 202, 1515
CsiI ACCWGGT 1 cut(s) 2234
Csp6I GTAC 9 cut(s) 917, 951, 1541, 1715, 1778, 2133, 2317, 2364, 2611
CspI CGGWCCG 1 cut(s) 442
CviQI GTAC 9 cut(s) 917, 951, 1541, 1715, 1778, 2133, 2317, 2364, 2611
DdeI CTNAG 7 cut(s) 642, 740, 1160, 1303, 1340, 1395, 2283
DpnI GATC 7 cut(s) 147, 270, 378, 672, 1245, 1413, 2607
DpnII GATC 7 cut(s) 145, 268, 376, 670, 1243, 1411, 2605
DraI TTTAAA 1 cut(s) 15
DraIII CACNNNGTG 1 cut(s) 1610
DriI GACNNNNNGTC 1 cut(s) 2464
EaeI YGGCCR 3 cut(s) 295, 1807, 2761
EagI CGGCCG 1 cut(s) 2761
Eam1104I CTCTTC 5 cut(s) 92, 393, 1888, 2088, 2261
Eam1105I GACNNNNNGTC 1 cut(s) 2464
EarI CTCTTC 5 cut(s) 92, 393, 1888, 2088, 2261
Ecl136II GAGCTC 2 cut(s) 288, 2618
EclXI CGGCCG 1 cut(s) 2761
Eco105I TACGTA 1 cut(s) 328
Eco130I CCWWGG 3 cut(s) 1273, 1722, 1800
Eco24I GRGCYC 2 cut(s) 290, 2620
Eco32I GATATC 1 cut(s) 1391
Eco47I GGWCC 2 cut(s) 442, 2812
Eco52I CGGCCG 1 cut(s) 2761
Eco53kI GAGCTC 2 cut(s) 288, 2618
Eco57I CTGAAG 4 cut(s) 437, 1071, 1240, 1680
Eco72I CACGTG 1 cut(s) 1607
Eco81I CCTNAGG 1 cut(s) 1395
Eco91I GGTNACC 1 cut(s) 830
EcoICRI GAGCTC 2 cut(s) 288, 2618
EcoO109I RGGNCCY 1 cut(s) 2812
EcoO65I GGTNACC 1 cut(s) 830
EcoRI GAATTC 1 cut(s) 435
EcoRII CCWGG 6 cut(s) 919, 1246, 2160, 2234, 2484, 2690
EcoRV GATATC 1 cut(s) 1391
EcoT14I CCWWGG 3 cut(s) 1273, 1722, 1800
EcoT22I ATGCAT 1 cut(s) 1697
EcoT38I GRGCYC 2 cut(s) 290, 2620
ErhI CCWWGG 3 cut(s) 1273, 1722, 1800
Esp3I CGTCTC 1 cut(s) 185
FalI AAGNNNNNCTT 4 cut(s) 1583, 1615, 2091, 2123
FaqI GGGAC 3 cut(s) 955, 2468, 2798
FauI CCCGC 3 cut(s) 441, 2445, 2511
FbaI TGATCA 1 cut(s) 145
FblI GTMKAC 1 cut(s) 716
FriOI GRGCYC 2 cut(s) 290, 2620
FspBI CTAG 7 cut(s) 21, 341, 555, 591, 1032, 1128, 1652
GlaI GCGC 2 cut(s) 1087, 2623
GsaI CCCAGC 1 cut(s) 1371
GsuI CTGGAG 2 cut(s) 979, 2144
HaeIII GGCC 5 cut(s) 297, 319, 1809, 2386, 2763
HapII CCGG 8 cut(s) 261, 291, 316, 409, 440, 445, 1495, 1510
HgaI GACGC 2 cut(s) 202, 1515
HhaI GCGC 2 cut(s) 1088, 2624
Hin1I GRCGYC 2 cut(s) 174, 1507
Hin6I GCGC 2 cut(s) 1086, 2622
HinP1I GCGC 2 cut(s) 1086, 2622
HindIII AAGCTT 2 cut(s) 569, 2399
HpaII CCGG 8 cut(s) 261, 291, 316, 409, 440, 445, 1495, 1510
HphI GGTGA 7 cut(s) 205, 250, 2197, 2264, 2360, 2426, 2549
Hpy166II GTNNAC 8 cut(s) 717, 951, 1330, 1780, 2319, 2330, 2350, 2434
Hpy188III TCNNGA 7 cut(s) 143, 804, 839, 1284, 1394, 1625, 2244
Hpy8I GTNNAC 8 cut(s) 717, 951, 1330, 1780, 2319, 2330, 2350, 2434
Hpy99I CGWCG 4 cut(s) 176, 179, 1042, 1554
HpyAV CCTTC 6 cut(s) 950, 1046, 1063, 1655, 2284, 2783
HpyCH4III ACNGT 4 cut(s) 836, 1760, 2492, 2800
HpyCH4IV ACGT 8 cut(s) 174, 327, 703, 808, 1040, 1606, 1870, 2073
HpyF10VI GCNNNNNNNGC 7 cut(s) 113, 270, 294, 1652, 2108, 2268, 2766
HpyF3I CTNAG 7 cut(s) 642, 740, 1160, 1303, 1340, 1395, 2283
HpySE526I ACGT 8 cut(s) 174, 327, 703, 808, 1040, 1606, 1870, 2073
Hsp92I GRCGYC 2 cut(s) 174, 1507
HspAI GCGC 2 cut(s) 1086, 2622
Ksp22I TGATCA 1 cut(s) 145
Kzo9I GATC 7 cut(s) 145, 268, 376, 670, 1243, 1411, 2605
LguI GCTCTTC 1 cut(s) 2261
LmnI GCTCC 4 cut(s) 293, 1224, 2031, 2163
LweI GCATC 3 cut(s) 1925, 2434, 2778
MabI ACCWGGT 1 cut(s) 2234
MaeI CTAG 7 cut(s) 21, 341, 555, 591, 1032, 1128, 1652
MaeII ACGT 8 cut(s) 174, 327, 703, 808, 1040, 1606, 1870, 2073
MaeIII GTNAC 5 cut(s) 251, 830, 1250, 2197, 2634
MalI GATC 7 cut(s) 147, 270, 378, 672, 1245, 1413, 2607
MboI GATC 7 cut(s) 145, 268, 376, 670, 1243, 1411, 2605
MfeI CAATTG 1 cut(s) 2040
MflI RGATCY 2 cut(s) 376, 1411
MhlI GDGCHC 6 cut(s) 290, 1135, 2028, 2332, 2620, 2793
MlsI TGGCCA 1 cut(s) 1809
MluNI TGGCCA 1 cut(s) 1809
MlyI GAGTC 2 cut(s) 397, 937
MmeI TCCRAC 6 cut(s) 889, 1014, 1120, 1532, 1966, 2251
Mox20I TGGCCA 1 cut(s) 1809
Mph1103I ATGCAT 1 cut(s) 1697
MscI TGGCCA 1 cut(s) 1809
MseI TTAA 7 cut(s) 14, 426, 567, 573, 1740, 1767, 2166
MslI CAYNNNNRTG 3 cut(s) 1430, 1972, 2601
Msp20I TGGCCA 1 cut(s) 1809
MspA1I CMGCKG 2 cut(s) 2215, 2324
MspCI CTTAAG 1 cut(s) 566
MspI CCGG 8 cut(s) 261, 291, 316, 409, 440, 445, 1495, 1510
MspR9I CCNGG 6 cut(s) 921, 1248, 2162, 2236, 2486, 2692
MunI CAATTG 1 cut(s) 2040
MvaI CCWGG 6 cut(s) 921, 1248, 2162, 2236, 2486, 2692
MvnI CGCG 2 cut(s) 1088, 2622
MwoI GCNNNNNNNGC 7 cut(s) 113, 270, 294, 1652, 2108, 2268, 2766
NdeII GATC 7 cut(s) 145, 268, 376, 670, 1243, 1411, 2605
NlaIV GGNNCC 5 cut(s) 209, 1271, 2159, 2572, 2814
NsiI ATGCAT 1 cut(s) 1697
NspI RCATGY 4 cut(s) 1098, 1353, 1715, 1977
PagI TCATGA 1 cut(s) 1624
PciI ACATGT 1 cut(s) 1349
PciSI GCTCTTC 1 cut(s) 2261
PflFI GACNNNGTC 1 cut(s) 2236
PflMI CCANNNNNTGG 1 cut(s) 2691
PfoI TCCNGGA 1 cut(s) 2484
Ple19I CGATCG 1 cut(s) 271
PleI GAGTC 2 cut(s) 397, 937
PmaCI CACGTG 1 cut(s) 1607
PmlI CACGTG 1 cut(s) 1607
PpsI GAGTC 2 cut(s) 397, 937
Ppu21I YACGTR 3 cut(s) 328, 1607, 2074
PpuMI RGGWCCY 1 cut(s) 2812
PscI ACATGT 1 cut(s) 1349
Psp124BI GAGCTC 2 cut(s) 290, 2620
Psp5II RGGWCCY 1 cut(s) 2812
Psp6I CCWGG 6 cut(s) 919, 1246, 2160, 2234, 2484, 2690
PspCI CACGTG 1 cut(s) 1607
PspEI GGTNACC 1 cut(s) 830
PspFI CCCAGC 1 cut(s) 1367
PspGI CCWGG 6 cut(s) 919, 1246, 2160, 2234, 2484, 2690
PspN4I GGNNCC 5 cut(s) 209, 1271, 2159, 2572, 2814
PspPI GGNCC 3 cut(s) 318, 442, 2812
PspPPI RGGWCCY 1 cut(s) 2812
PsuI RGATCY 2 cut(s) 376, 1411
PsyI GACNNNGTC 1 cut(s) 2236
PvuI CGATCG 1 cut(s) 271
PvuII CAGCTG 2 cut(s) 2215, 2324
RgaI GCGATCGC 1 cut(s) 271
RsaI GTAC 9 cut(s) 918, 952, 1542, 1716, 1779, 2134, 2318, 2365, 2612
RsaNI GTAC 9 cut(s) 917, 951, 1541, 1715, 1778, 2133, 2317, 2364, 2611
RseI CAYNNNNRTG 3 cut(s) 1430, 1972, 2601
Rsr2I CGGWCCG 1 cut(s) 442
RsrII CGGWCCG 1 cut(s) 442
SacI GAGCTC 2 cut(s) 290, 2620
SapI GCTCTTC 1 cut(s) 2261
SaqAI TTAA 7 cut(s) 14, 426, 567, 573, 1740, 1767, 2166
Sau3AI GATC 7 cut(s) 145, 268, 376, 670, 1243, 1411, 2605
Sau96I GGNCC 3 cut(s) 318, 442, 2812
ScaI AGTACT 2 cut(s) 2134, 2365
SchI GAGTC 2 cut(s) 397, 937
ScrFI CCNGG 6 cut(s) 921, 1248, 2162, 2236, 2486, 2692
SduI GDGCHC 6 cut(s) 290, 1135, 2028, 2332, 2620, 2793
SexAI ACCWGGT 1 cut(s) 2234
SfaAI GCGATCGC 1 cut(s) 271
SfaNI GCATC 3 cut(s) 1925, 2434, 2778
SgfI GCGATCGC 1 cut(s) 271
SgrAI CRCCGGYG 1 cut(s) 260
SinI GGWCC 2 cut(s) 442, 2812
SmiMI CAYNNNNRTG 3 cut(s) 1430, 1972, 2601
SmlI CTYRAG 4 cut(s) 566, 839, 1165, 2462
SmoI CTYRAG 4 cut(s) 566, 839, 1165, 2462
SnaBI TACGTA 1 cut(s) 328
SpeI ACTAGT 1 cut(s) 1127
SsiI CCGC 8 cut(s) 264, 298, 448, 1088, 1355, 2438, 2504, 2760
SspI AATATT 2 cut(s) 1322, 2511
SspMI CTAG 7 cut(s) 21, 341, 555, 591, 1032, 1128, 1652
SstI GAGCTC 2 cut(s) 290, 2620
StyD4I CCNGG 6 cut(s) 919, 1246, 2160, 2234, 2484, 2690
StyI CCWWGG 3 cut(s) 1273, 1722, 1800
TaaI ACNGT 4 cut(s) 836, 1760, 2492, 2800
TaiI ACGT 8 cut(s) 177, 330, 706, 811, 1043, 1609, 1873, 2076
TaqI TCGA 5 cut(s) 334, 401, 1736, 1932, 2243
TatI WGTACW 6 cut(s) 950, 1777, 2132, 2316, 2363, 2610
TauI GCSGC 3 cut(s) 267, 300, 2763
Tru1I TTAA 7 cut(s) 14, 426, 567, 573, 1740, 1767, 2166
Tru9I TTAA 7 cut(s) 14, 426, 567, 573, 1740, 1767, 2166
TscAI CASTG 6 cut(s) 170, 1003, 2337, 2360, 2692, 2803
TspRI CASTG 6 cut(s) 170, 1003, 2337, 2360, 2692, 2803
Tth111I GACNNNGTC 1 cut(s) 2236
Van91I CCANNNNNTGG 1 cut(s) 2691
Vha464I CTTAAG 1 cut(s) 566
VneI GTGCAC 1 cut(s) 2328
VpaK11BI GGWCC 2 cut(s) 442, 2812
XapI RAATTY 6 cut(s) 435, 790, 1108, 1218, 1418, 1732
XceI RCATGY 4 cut(s) 1098, 1353, 1715, 1977
XmiI GTMKAC 1 cut(s) 716
XspI CTAG 7 cut(s) 21, 341, 555, 591, 1032, 1128, 1652
ZraI GACGTC 1 cut(s) 175
ZrmI AGTACT 2 cut(s) 2134, 2365
Zsp2I ATGCAT 1 cut(s) 1697
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.