Rh5DG243700

Protein of unknown function (DUF2921)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000420
Physical Location & Seq
Reverse (-)
27888896 .. 27891631
2736 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG243700.1

Sequence Viewer

Length: 2736 bp
ATGACCATCTCCATGAAGCTTTTCATATCCTTCTTTGTTTTTACCACCATTTCCTTCAATTCATTGTCGTTCTCTGCTTCAGCTGCTCTAATCTCTTATGCTGACCATTGTGCTTCAGTTGTTCCCGAGTCAACCGAAAATATAAATGCAGGATCTCAGTTTGTACATCTGATTCACACAGGCTACTACTACACTGGTGGTGGCAGTGACATTCTTAATCTATACTCGCCCGATGATCAGGTCAAAAATTCAGTTGGCTTCCAATGCTGGGATATTAGTGAAACTCATGTACAAGGCTTGTTTAAGGTCGAGGCAACTCTAGAGTTTCAAGTAACCAATATGATTGAGTTGCAAAGCATGCCGCCTATAATAATTCCAGGTTCGGTTTCATTTAGACTGAATGGATTCTGGTCAAAATCTTCTGGGAAGCTCTGCATGGTTGGATCATCGTCCATTTACTCGAAACAAGGTAATCCACTTGTTGTTCTTAAGCTGTATAATCTCATCATGAATAACACTAGTGTTACAAGTTTGATTAGTGGAACCTTTGAGAATGTCAAGATGATATCTGAGAATGATCCCAAGTCTTTGGGACCAATCTCTATTTTGATGTTTCCTCGCATGAACTACGCGTACACTTTGGTCTCAAATAGTACTAATAGCAGTTGCTCTAGTGGAAGTGAAGATACCCCACGCTCTTTACATGTTGAGAGATTTTGTTCACTACTATCATCAATAGTACTAAATCATGACTTTCCACTCAAATTTTCAAGCCACTGTATCACTCCAGAGAACTGCATTCCAATTTCTATGTCTAATTTACCTCGGGCTGTGTTGTTTAAGGACATTGCATGCTCGGAGGATAATCGAAGGGTGAGAGTTCTGGTGGAATTTTCTGATGGTAGGCTAAACCAGTGCCCAACGCTCTTCAATCCTAATACAACTTTAGTTGGGGAAGGCTCATGGGATGCAAAGAAGAATCAGCTATGTGTTGTTGCTTGTCGATTCTTGAATACAACAAATTCTTTGAATAATACTAATGTGGGTGATTGTTCAACAAGATTGAGCTTGAGAATTCCTGCAACAGGGACAATTGGAAGTACTGGCAGCATTGTGGGGCATATTTGGAGCACCAAAACTGTGACAGAGTTGGGCTACTTTGAAAAGATCACATTTGCAAGTCGGGACTGTGGTTGTCGCAAAGGTCTACTTCCAGGCCAGACATATGAGTACACTAAAATGGAGAAAGTAACAAACTTGTGCTCGAGAAAGAAGACTGCAAATGACATGGCCAGCATTTACCCAAATCCATTTTTGCATGTCACGAGTTTCATTATGCATGCCGAAAATTCTGAAGGACAAGTTGGATTTGGCAATTGTGTTCCTCTCTCTGTTGACAATAGTATTTACCGGACTGATTGGTCTTCGGGTGAAGATGCATGTGCACCTGAAAGCAATCAAGAATACTCTGTGGCTCCTCTGACTTACAGCTACAACCACAGCAATCCCATCAACATTAGCTACAAAATAGATCTCCAACTACAATCTAATCCAAAGTGGGGCAAGCAGTCGGTTAAATATGAAATGCATATCAATGCTGAAGGGATCTATGATGGTACAGAAGGAAGCCTTTGCATGGTAGGCTGCCGCAAACTTGTCTCAAACAGTCAGCAGGCAACAAATGATTCAATAGATTGTGAGATTCTTGTCCATTTTCAGTTCCCAGCAACAACTGCAAACGATTTGCATCATATCAAAGGAAGCATTGAGAGCACTCGAAAGAAGTCCGATCCTCTCCATTTTGAACGCTTGGATTTGTCTACAGCTGCCAAGCATGATGAAGAAAAGCGATCCATTTGGAGGATGGATGGGAAGATAACCTTGGTTCTTATATCAACCACACTTGCATGTGTTTTTGCTGCATTACAAATCTTCCTTGTGAAAAGAAACCCTGAAGTCATTCCCTCTATTTCCATCTTAATGCTTCTAATTCTAACCCTTAGCTACATGTTACCTCTAGGGATTAATATTGAAGCCATGTTCATGCATAATTTCAACCGCCAAAATGTGCACCTTGGAGGGGGTGGATGGCTTGAATTTAATGAGATAATCATAAGGGTGGCAACATTGGTAGCTTTCTTGATACAGTTCCACCTTCTGAAGCTAACTTGGTCAGCAAAATTCGGAAATGGAACCCGGAAGGATCTCCGGGTTATGGAAAAGAGGGCTCTCTTTGTGGCTTTACAAGTGTATGCTGTAGGGGCTTTAGCTGCTTTGTTTCTGCATAGACAGAAACTGAGGAAGAGGGACGATCGCATTGAGTTGCCTCATCTAGAGAATACAGTTCTGGATGCCTTGACATCTTATGGTTGTTTGGTCTTGGATAGCTTTTTGTTCCCTCAAATTCTGCTCAACATATTCTGCCAATCGAAGGAAAATGCTCTATCAGTTTGGTTCTACATTGGAACAACTGTAGCTCGAGTTCTGCCACATGCACATGCTCTTTACAGGGATCACAGCCGGGCCAATGACCAATTTGATGATTTCTCATACAATTATCCAGCTCCTGTGGCAGATTTATACTCCATAGCTTGGAATGCCATCATTCCTTTAGAAGGTGTACTGTTTGCTGGTATCATATACTTGCAGCAGAGGTTTGGCGGTCGTTGCATTGCTCCTGGAAAGCTGCGAGAGGTGGGTATGTATAAGAAGGTCCCTACAGTTCATGAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
DUF2921

Protein Analysis

911

Amino Acids

101.57

Weight (kDa)

7.1

Isoelectric Point (pI)

39.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2921_N PF25333 33 - 201 6.6e-17 DUF2921 lipocalin-like domain
DUF2921_N PF25333 308 - 391 5.5e-25 DUF2921 lipocalin-like domain
DUF2921_N PF25333 421 - 605 2.1e-31 DUF2921 lipocalin-like domain
DUF2921 PF11145 616 - 895 6.4e-53 Transmembrane E3 ligase/DUF2921 transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21700
fragaria_vesca FvH4_1g10360 FvH4_1g10760 FvH4_2g30370 FvH4_3g09240 FvH4_3g20050 FvH4_3g20280 FvH4_3g20290 FvH4_3g20291 FvH4_3g20340 FvH4_3g20350 FvH4_3g20351 FvH4_3g20380 FvH4_3g20390 FvH4_5g13190 FvH4_6g20070 FvH4_6g42890
malus_domestica MD03G1227000.v1.1 MD10G1294900.v1.1 MD11G1246300.v1.1 MD11G1246400.v1.1 MD11G1246500.v1.1 MD11G1246600.v1.1
prunus_persica Prupe.4G047300_v2.0.a1 Prupe.4G181700_v2.0.a1 Prupe.4G181900_v2.0.a1 Prupe.4G182000_v2.0.a1 Prupe.4G182200_v2.0.a1
pyrus_communis pycom03g17470 pycom10g24670 pycom11g21640
rosa_chinensis RchiOBHm_Chr5g0008381 RchiOBHm_Chr5g0008391 RchiOBHm_Chr5g0033881 RchiOBHm_Chr5g0034171 RchiOBHm_Chr5g0034181 RchiOBHm_Chr5g0034191 RchiOBHm_Chr5g0034201 RchiOBHm_Chr5g0034211 RchiOBHm_Chr5g0034221 RchiOBHm_Chr5g0034231 RchiOBHm_Chr5g0034271 RchiOBHm_Chr5g0034281
rosa_laevigata RLG00000031566 RLG00000031567 RLG00000033506 RLG00000033529 RLG00000033530 RLG00000033531
rosa_multiflora Rmu_co8445509.1_g000001 Rmu_co8470615.1_g000001 Rmu_sc0008805.1_g000003 Rmu_sc0008805.1_g000004 Rmu_ssc0000128.1_g000029 Rmu_ssc0000128.1_g000031 Rmu_ssc0000128.1_g000032 Rmu_ssc0000388.1_g000062 Rmu_ssc0000388.1_g000063
rosa_roxburghii Rroxscaffold_1G00046010 Rroxscaffold_1G00046020 Rroxscaffold_1G00046030 Rroxscaffold_1G00046040 Rroxscaffold_1G00046060 Rroxscaffold_1G00046070 Rroxscaffold_1G00046080 Rroxscaffold_1G00046090
rosa_rugosa Rorug04G0435500 Rorug05G0142900 Rorug05G0144600 Rorug05G0144600 Rorug05G0144700 Rorug05G0144800
rosa_samantha Rh5AG233800 Rh5AG235900 Rh5AG236000 Rh5AG236100 Rh5AG236200 Rh5AG236300 Rh5AG236500 Rh5BG061200 Rh5BG061300 Rh5BG234300 Rh5BG236600 Rh5BG236800 Rh5CG071200 Rh5DG060000 Rh5DG060100 Rh5DG241300 Rh5DG243700 Rh5DG244000 Rh5DG244100
rosa_wichuraiana Rw3G018950 Rw5G005680 Rw5G021330 Rw5G021340 Rw5G021350 Rw5G021550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1420
AccB7I CCANNNNNTGG 1 cut(s) 2592
AccI GTMKAC 2 cut(s) 1207, 1820
AccII CGCG 1 cut(s) 632
AciI CCGC 4 cut(s) 362, 1648, 2059, 2661
AclWI GGATC 8 cut(s) 160, 451, 572, 1613, 1784, 1845, 2211, 2520
AcoI YGGCCR 1 cut(s) 1290
AcsI RAATTY 9 cut(s) 247, 764, 890, 1021, 1074, 1348, 2096, 2180, 2403
AcuI CTGAAG 6 cut(s) 63, 99, 1374, 1620, 1974, 2180
AfaI GTAC 9 cut(s) 165, 291, 635, 655, 741, 1102, 1232, 1618, 2622
AflII CTTAAG 1 cut(s) 488
AflIII ACRYGT 3 cut(s) 630, 703, 2007
AhlI ACTAGT 1 cut(s) 518
AjnI CCWGG 3 cut(s) 376, 1213, 2677
AjuI GAANNNNNNNTTGG 4 cut(s) 1347, 1379, 1865, 1897
Alw21I GWGCWC 5 cut(s) 1133, 1265, 1447, 1775, 2073
Alw26I GTCTC 2 cut(s) 649, 1663
Alw44I GTGCAC 2 cut(s) 1443, 2069
AlwI GGATC 8 cut(s) 160, 451, 572, 1613, 1784, 1845, 2211, 2520
AlwNI CAGNNNCTG 2 cut(s) 2296, 2567
Ama87I CYCGRG 4 cut(s) 125, 825, 1264, 2478
AoxI GGCC 3 cut(s) 1216, 1290, 2523
ApaLI GTGCAC 2 cut(s) 1443, 2069
ApeKI GCWGC 8 cut(s) 83, 1107, 1644, 1826, 1919, 2270, 2647, 2686
ApoI RAATTY 9 cut(s) 247, 764, 890, 1021, 1074, 1348, 2096, 2180, 2403
AseI ATTAAT 1 cut(s) 2025
Asp700I GAANNNNTTC 3 cut(s) 20, 404, 1959
AspS9I GGNCC 3 cut(s) 593, 2523, 2713
AsuC2I CCSGG 3 cut(s) 2197, 2210, 2522
AsuHPI GGTGA 3 cut(s) 886, 1058, 1442
AvaI CYCGRG 4 cut(s) 125, 825, 1264, 2478
AvaII GGWCC 2 cut(s) 593, 2713
BaeGI GKGCMC 3 cut(s) 920, 1447, 2073
BalI TGGCCA 1 cut(s) 1292
BanII GRGCYC 1 cut(s) 2230
BarI GAAGNNNNNNTAC 2 cut(s) 670, 702
BauI CACGAG 1 cut(s) 1324
BbsI GAAGAC 2 cut(s) 1280, 1416
Bbv12I GWGCWC 5 cut(s) 1133, 1265, 1447, 1775, 2073
BbvI GCAGC 8 cut(s) 70, 1119, 1631, 1813, 1906, 2257, 2659, 2673
BccI CCATC 9 cut(s) 14, 893, 1517, 1607, 1858, 1862, 1982, 2082, 2609
BciT130I CCWGG 3 cut(s) 378, 1215, 2679
BclI TGATCA 1 cut(s) 235
BcnI CCSGG 3 cut(s) 2197, 2210, 2522
BcoDI GTCTC 2 cut(s) 649, 1663
BcuI ACTAGT 1 cut(s) 518
BfaI CTAG 5 cut(s) 320, 519, 672, 2018, 2333
BfmI CTRYAG 4 cut(s) 1821, 2256, 2472, 2718
BfrI CTTAAG 1 cut(s) 488
BglII AGATCT 1 cut(s) 1531
BmcAI AGTACT 3 cut(s) 655, 741, 1102
Bme1390I CCNGG 6 cut(s) 378, 1215, 2197, 2210, 2522, 2679
Bme18I GGWCC 2 cut(s) 593, 2713
BmeT110I CYCGRG 4 cut(s) 125, 825, 1264, 2478
BmgT120I GGNCC 3 cut(s) 593, 2523, 2713
BmiI GGNNCC 5 cut(s) 544, 594, 1476, 2194, 2715
BmrFI CCNGG 6 cut(s) 378, 1215, 2197, 2210, 2522, 2679
BmsI GCATC 4 cut(s) 958, 1426, 1756, 2341
BpiI GAAGAC 2 cut(s) 1280, 1416
BpmI CTGGAG 1 cut(s) 771
Bpu10I CCTNAGC 1 cut(s) 2000
BpuEI CTTGAG 1 cut(s) 1090
BpuMI CCSGG 3 cut(s) 2197, 2210, 2522
BsaBI GATNNNNATC 1 cut(s) 1746
BsaI GGTCTC 1 cut(s) 649
BsaJI CCNNGG 3 cut(s) 824, 1881, 2074
BsaWI WCCGGW 1 cut(s) 1410
Bse1I ACTGG 3 cut(s) 199, 913, 1108
Bse3DI GCAATG 2 cut(s) 846, 2670
Bse8I GATNNNNATC 1 cut(s) 1746
BseBI CCWGG 3 cut(s) 378, 1215, 2679
BseDI CCNNGG 3 cut(s) 824, 1881, 2074
BseGI GGATG 5 cut(s) 973, 1869, 1873, 2093, 2356
BseJI GATNNNNATC 1 cut(s) 1746
BseMI GCAATG 2 cut(s) 846, 2670
BseMII CTCAG 3 cut(s) 170, 561, 2288
BseNI ACTGG 3 cut(s) 199, 913, 1108
BseRI GAGGAG 1 cut(s) 1467
BseSI GKGCMC 3 cut(s) 920, 1447, 2073
BseXI GCAGC 8 cut(s) 70, 1119, 1631, 1813, 1906, 2257, 2659, 2673
BseYI CCCAGC 2 cut(s) 267, 1723
Bsh1236I CGCG 1 cut(s) 632
Bsh1285I CGRYCG 2 cut(s) 2314, 2665
BshFI GGCC 3 cut(s) 1218, 1292, 2525
BsiEI CGRYCG 2 cut(s) 2314, 2665
BsiHKAI GWGCWC 5 cut(s) 1133, 1265, 1447, 1775, 2073
BsiHKCI CYCGRG 4 cut(s) 125, 825, 1264, 2478
BsiSI CCGG 4 cut(s) 1411, 2197, 2209, 2521
BslFI GGGAC 5 cut(s) 606, 1102, 1199, 2321, 2699
BsmAI GTCTC 2 cut(s) 649, 1663
BsmFI GGGAC 5 cut(s) 606, 1102, 1199, 2321, 2699
BsmI GAATGC 2 cut(s) 798, 2602
BsnI GGCC 3 cut(s) 1218, 1292, 2525
Bso31I GGTCTC 1 cut(s) 649
BsoBI CYCGRG 4 cut(s) 125, 825, 1264, 2478
Bsp1286I GDGCHC 7 cut(s) 920, 1133, 1265, 1447, 1775, 2073, 2230
Bsp1407I TGTACA 2 cut(s) 163, 289
BspACI CCGC 4 cut(s) 362, 1648, 2059, 2661
BspANI GGCC 3 cut(s) 1218, 1292, 2525
BspCNI CTCAG 3 cut(s) 169, 562, 2289
BspFNI CGCG 1 cut(s) 632
BspHI TCATGA 3 cut(s) 507, 748, 2725
BspLI GGNNCC 5 cut(s) 544, 594, 1476, 2194, 2715
BspPI GGATC 8 cut(s) 160, 451, 572, 1613, 1784, 1845, 2211, 2520
BspQI GCTCTTC 1 cut(s) 932
BspTI CTTAAG 1 cut(s) 488
BspTNI GGTCTC 1 cut(s) 649
BsrDI GCAATG 2 cut(s) 846, 2670
BsrGI TGTACA 2 cut(s) 163, 289
BsrI ACTGG 3 cut(s) 199, 913, 1108
BssECI CCNNGG 3 cut(s) 824, 1881, 2074
BssSI CACGAG 1 cut(s) 1324
BssT1I CCWWGG 2 cut(s) 1881, 2074
Bst2BI CACGAG 1 cut(s) 1324
Bst2UI CCWGG 3 cut(s) 378, 1215, 2679
Bst4CI ACNGT 9 cut(s) 779, 1141, 1190, 1667, 2148, 2344, 2473, 2625, 2722
Bst6I CTCTTC 3 cut(s) 932, 2297, 2724
BstAFI CTTAAG 1 cut(s) 488
BstAPI GCANNNNNTGC 2 cut(s) 358, 1733
BstAUI TGTACA 2 cut(s) 163, 289
BstC8I GCNNGC 6 cut(s) 359, 853, 1294, 1341, 1565, 1674
BstDEI CTNAG 4 cut(s) 156, 570, 2000, 2297
BstENI CCTNNNNNAGG 2 cut(s) 1083, 2613
BstF5I GGATG 5 cut(s) 973, 1869, 1873, 2093, 2356
BstFNI CGCG 1 cut(s) 632
BstMAI GTCTC 2 cut(s) 649, 1663
BstMCI CGRYCG 2 cut(s) 2314, 2665
BstNI CCWGG 3 cut(s) 378, 1215, 2679
BstSCI CCNGG 6 cut(s) 376, 1213, 2195, 2208, 2520, 2677
BstSFI CTRYAG 4 cut(s) 1821, 2256, 2472, 2718
BstSLI GKGCMC 3 cut(s) 920, 1447, 2073
BstUI CGCG 1 cut(s) 632
BstV1I GCAGC 8 cut(s) 70, 1119, 1631, 1813, 1906, 2257, 2659, 2673
BstV2I GAAGAC 2 cut(s) 1280, 1416
BstX2I RGATCY 4 cut(s) 152, 1531, 1605, 2203
BstXI CCANNNNNNTGG 1 cut(s) 589
BstYI RGATCY 4 cut(s) 152, 1531, 1605, 2203
BsuRI GGCC 3 cut(s) 1218, 1292, 2525
BtsCI GGATG 5 cut(s) 973, 1869, 1873, 2093, 2356
BtsI GCAGTG 1 cut(s) 211
BtsIMutI CAGTG 4 cut(s) 192, 211, 775, 920
Cac8I GCNNGC 6 cut(s) 359, 853, 1294, 1341, 1565, 1674
CaiI CAGNNNCTG 2 cut(s) 2296, 2567
CciI TCATGA 3 cut(s) 507, 748, 2725
Cfr13I GGNCC 3 cut(s) 593, 2523, 2713
Csp6I GTAC 9 cut(s) 164, 290, 634, 654, 740, 1101, 1231, 1617, 2621
CviQI GTAC 9 cut(s) 164, 290, 634, 654, 740, 1101, 1231, 1617, 2621
DdeI CTNAG 4 cut(s) 156, 570, 2000, 2297
DrdI GACNNNNNNGTC 1 cut(s) 1420
DseDI GACNNNNNNGTC 1 cut(s) 1420
EaeI YGGCCR 1 cut(s) 1290
Eam1104I CTCTTC 3 cut(s) 932, 2297, 2724
EarI CTCTTC 3 cut(s) 932, 2297, 2724
Eco130I CCWWGG 2 cut(s) 1881, 2074
Eco24I GRGCYC 1 cut(s) 2230
Eco31I GGTCTC 1 cut(s) 649
Eco32I GATATC 1 cut(s) 567
Eco47I GGWCC 2 cut(s) 593, 2713
Eco57I CTGAAG 6 cut(s) 63, 99, 1374, 1620, 1974, 2180
Eco88I CYCGRG 4 cut(s) 125, 825, 1264, 2478
EcoNI CCTNNNNNAGG 2 cut(s) 1083, 2613
EcoO109I RGGNCCY 1 cut(s) 2713
EcoRI GAATTC 1 cut(s) 1074
EcoRII CCWGG 3 cut(s) 376, 1213, 2677
EcoRV GATATC 1 cut(s) 567
EcoT14I CCWWGG 2 cut(s) 1881, 2074
EcoT22I ATGCAT 4 cut(s) 1341, 1441, 1590, 2049
EcoT38I GRGCYC 1 cut(s) 2230
ErhI CCWWGG 2 cut(s) 1881, 2074
FalI AAGNNNNNCTT 6 cut(s) 1194, 1226, 1614, 1646, 1865, 1897
FaqI GGGAC 5 cut(s) 606, 1102, 1199, 2321, 2699
FauNDI CATATG 1 cut(s) 1225
FbaI TGATCA 1 cut(s) 235
FblI GTMKAC 2 cut(s) 1207, 1820
FokI GGATG 5 cut(s) 980, 1876, 1880, 2100, 2363
FriOI GRGCYC 1 cut(s) 2230
FspBI CTAG 5 cut(s) 320, 519, 672, 2018, 2333
GsaI CCCAGC 2 cut(s) 271, 1727
GsuI CTGGAG 1 cut(s) 771
HaeIII GGCC 3 cut(s) 1218, 1292, 2525
HapII CCGG 4 cut(s) 1411, 2197, 2209, 2521
HincII GTYRAC 2 cut(s) 132, 1396
HindII GTYRAC 2 cut(s) 132, 1396
HindIII AAGCTT 1 cut(s) 17
HinfI GANTC 7 cut(s) 128, 172, 405, 979, 1005, 1685, 1702
HpaII CCGG 4 cut(s) 1411, 2197, 2209, 2521
HphI GGTGA 3 cut(s) 886, 1058, 1442
Hpy188I TCNGA 9 cut(s) 171, 571, 859, 898, 1354, 1482, 1789, 2160, 2186
HpyCH4III ACNGT 9 cut(s) 779, 1141, 1190, 1667, 2148, 2344, 2473, 2625, 2722
HpyF3I CTNAG 4 cut(s) 156, 570, 2000, 2297
Ksp22I TGATCA 1 cut(s) 235
LguI GCTCTTC 1 cut(s) 932
LmnI GCTCC 4 cut(s) 1128, 1480, 2569, 2680
Lsp1109I GCAGC 8 cut(s) 70, 1119, 1631, 1813, 1906, 2257, 2659, 2673
LweI GCATC 4 cut(s) 958, 1426, 1756, 2341
MaeI CTAG 5 cut(s) 320, 519, 672, 2018, 2333
MaeIII GTNAC 7 cut(s) 206, 331, 523, 1141, 1249, 1321, 2010
MfeI CAATTG 2 cut(s) 1092, 1375
MflI RGATCY 4 cut(s) 152, 1531, 1605, 2203
MhlI GDGCHC 7 cut(s) 920, 1133, 1265, 1447, 1775, 2073, 2230
MlsI TGGCCA 1 cut(s) 1292
MluI ACGCGT 1 cut(s) 630
MluNI TGGCCA 1 cut(s) 1292
MlyI GAGTC 1 cut(s) 137
MmeI TCCRAC 3 cut(s) 421, 1345, 1561
Mox20I TGGCCA 1 cut(s) 1292
Mph1103I ATGCAT 4 cut(s) 1341, 1441, 1590, 2049
MroXI GAANNNNTTC 3 cut(s) 20, 404, 1959
MscI TGGCCA 1 cut(s) 1292
MseI TTAA 8 cut(s) 216, 303, 489, 840, 1575, 1979, 2025, 2100
MslI CAYNNNNRTG 8 cut(s) 11, 1238, 1593, 1906, 1979, 2042, 2117, 2496
Msp20I TGGCCA 1 cut(s) 1292
MspA1I CMGCKG 2 cut(s) 83, 1826
MspCI CTTAAG 1 cut(s) 488
MspI CCGG 4 cut(s) 1411, 2197, 2209, 2521
MspR9I CCNGG 6 cut(s) 378, 1215, 2197, 2210, 2522, 2679
MunI CAATTG 2 cut(s) 1092, 1375
Mva1269I GAATGC 2 cut(s) 798, 2602
MvaI CCWGG 3 cut(s) 378, 1215, 2679
MvnI CGCG 1 cut(s) 632
NciI CCSGG 3 cut(s) 2197, 2210, 2522
NdeI CATATG 1 cut(s) 1225
NlaIV GGNNCC 5 cut(s) 544, 594, 1476, 2194, 2715
NmuCI GTSAC 3 cut(s) 206, 1141, 1321
NsiI ATGCAT 4 cut(s) 1341, 1441, 1590, 2049
PaeI GCATGC 3 cut(s) 361, 855, 1343
PaeR7I CTCGAG 2 cut(s) 1264, 2478
PagI TCATGA 3 cut(s) 507, 748, 2725
PciI ACATGT 2 cut(s) 703, 2007
PciSI GCTCTTC 1 cut(s) 932
PctI GAATGC 2 cut(s) 798, 2602
PdmI GAANNNNTTC 3 cut(s) 20, 404, 1959
PfeI GAWTC 6 cut(s) 172, 405, 979, 1005, 1685, 1702
PflMI CCANNNNNTGG 1 cut(s) 2592
PfoI TCCNGGA 1 cut(s) 2677
Ple19I CGATCG 1 cut(s) 2314
PleI GAGTC 1 cut(s) 136
PpsI GAGTC 1 cut(s) 136
PpuMI RGGWCCY 1 cut(s) 2713
PscI ACATGT 2 cut(s) 703, 2007
PshBI ATTAAT 1 cut(s) 2025
Psp5II RGGWCCY 1 cut(s) 2713
Psp6I CCWGG 3 cut(s) 376, 1213, 2677
PspFI CCCAGC 2 cut(s) 267, 1723
PspGI CCWGG 3 cut(s) 376, 1213, 2677
PspN4I GGNNCC 5 cut(s) 544, 594, 1476, 2194, 2715
PspPI GGNCC 3 cut(s) 593, 2523, 2713
PspPPI RGGWCCY 1 cut(s) 2713
PspXI VCTCGAGB 1 cut(s) 2478
PsrI GAACNNNNNNTAC 4 cut(s) 617, 649, 2466, 2498
PstNI CAGNNNCTG 2 cut(s) 2296, 2567
PsuI RGATCY 4 cut(s) 152, 1531, 1605, 2203
PvuI CGATCG 1 cut(s) 2314
PvuII CAGCTG 2 cut(s) 83, 1826
RsaI GTAC 9 cut(s) 165, 291, 635, 655, 741, 1102, 1232, 1618, 2622
RsaNI GTAC 9 cut(s) 164, 290, 634, 654, 740, 1101, 1231, 1617, 2621
RseI CAYNNNNRTG 8 cut(s) 11, 1238, 1593, 1906, 1979, 2042, 2117, 2496
SapI GCTCTTC 1 cut(s) 932
SaqAI TTAA 8 cut(s) 216, 303, 489, 840, 1575, 1979, 2025, 2100
Sau96I GGNCC 3 cut(s) 593, 2523, 2713
ScaI AGTACT 3 cut(s) 655, 741, 1102
SchI GAGTC 1 cut(s) 137
ScrFI CCNGG 6 cut(s) 378, 1215, 2197, 2210, 2522, 2679
SduI GDGCHC 7 cut(s) 920, 1133, 1265, 1447, 1775, 2073, 2230
SfaNI GCATC 4 cut(s) 958, 1426, 1756, 2341
SfcI CTRYAG 4 cut(s) 1821, 2256, 2472, 2718
Sfr274I CTCGAG 2 cut(s) 1264, 2478
SinI GGWCC 2 cut(s) 593, 2713
SlaI CTCGAG 2 cut(s) 1264, 2478
SmiMI CAYNNNNRTG 8 cut(s) 11, 1238, 1593, 1906, 1979, 2042, 2117, 2496
SmlI CTYRAG 4 cut(s) 488, 1069, 1264, 2478
SmoI CTYRAG 4 cut(s) 488, 1069, 1264, 2478
SpeI ACTAGT 1 cut(s) 518
SphI GCATGC 3 cut(s) 361, 855, 1343
SsiI CCGC 4 cut(s) 362, 1648, 2059, 2661
SspI AATATT 1 cut(s) 2029
SspMI CTAG 5 cut(s) 320, 519, 672, 2018, 2333
StyD4I CCNGG 6 cut(s) 376, 1213, 2195, 2208, 2520, 2677
StyI CCWWGG 2 cut(s) 1881, 2074
TaaI ACNGT 9 cut(s) 779, 1141, 1190, 1667, 2148, 2344, 2473, 2625, 2722
TaqI TCGA 8 cut(s) 309, 461, 868, 1003, 1265, 1777, 2429, 2479
TatI WGTACW 7 cut(s) 163, 289, 653, 739, 1100, 1230, 2620
TauI GCSGC 2 cut(s) 364, 1650
TfiI GAWTC 6 cut(s) 172, 405, 979, 1005, 1685, 1702
Tru1I TTAA 8 cut(s) 216, 303, 489, 840, 1575, 1979, 2025, 2100
Tru9I TTAA 8 cut(s) 216, 303, 489, 840, 1575, 1979, 2025, 2100
TscAI CASTG 4 cut(s) 199, 211, 782, 920
TseFI GTSAC 3 cut(s) 206, 1141, 1321
TseI GCWGC 8 cut(s) 83, 1107, 1644, 1826, 1919, 2270, 2647, 2686
Tsp45I GTSAC 3 cut(s) 206, 1141, 1321
TspRI CASTG 4 cut(s) 199, 211, 782, 920
Van91I CCANNNNNTGG 1 cut(s) 2592
Vha464I CTTAAG 1 cut(s) 488
VneI GTGCAC 2 cut(s) 1443, 2069
VpaK11BI GGWCC 2 cut(s) 593, 2713
VspI ATTAAT 1 cut(s) 2025
XagI CCTNNNNNAGG 2 cut(s) 1083, 2613
XapI RAATTY 9 cut(s) 247, 764, 890, 1021, 1074, 1348, 2096, 2180, 2403
XbaI TCTAGA 2 cut(s) 319, 2332
XcmI CCANNNNNNNNNTGG 1 cut(s) 1861
XhoI CTCGAG 2 cut(s) 1264, 2478
XmiI GTMKAC 2 cut(s) 1207, 1820
XmnI GAANNNNTTC 3 cut(s) 20, 404, 1959
XspI CTAG 5 cut(s) 320, 519, 672, 2018, 2333
ZrmI AGTACT 3 cut(s) 655, 741, 1102
Zsp2I ATGCAT 4 cut(s) 1341, 1441, 1590, 2049
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.