Rh5AG236300

Protein of unknown function (DUF2921)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000420
Physical Location & Seq
Reverse (-)
28671088 .. 28676685
5598 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG236300.1

Sequence Viewer

Length: 3105 bp
ATGACCATCTCCATGAAGCTTTTCATATCCTTCTTTGTTTTTACCACCATTTCCTTCAATTCATTGTCGTTCTCTGCTTCAGCTTCTCTAATCTCTTATGCTGACCATTGTGCTTCAGTAGTTCCCGAGTCAACCGAAAATATAAATGCAGGATCTCAGTTTGTACATCTGATTCACACAGGCTACTACTACACTGGTGGTGGCAGTGACATTCTTAATCTATACTCGCCCGATGATCAGGTCAAAAATTCAGTTGGCTTCCAATGCTGGGATATTAGTGAAACTCATGTACAAGGCTTGTTTAAGGTCGAGGCAACTCTAGAGTTTCAAGTAACCAATATGATTGAGTTGCAAAGCATGCCGCCTAAAATAATTCCAGGTTCGGTTTCATTTAGACTCAATGGATTCTGGTCAAAATCTTCTGGGAAGCTCTGCATGGTTGGATCATCGTCCATTTACTCGAAACAAGGTAATCCACTTGTTGTCCTTAAGCTGTATAATCTCATCATGAATAACACTAGTGTTACAAGTTTGATTAGTGGAACCTTTGAGAATGTCATGATGATATCTGAGAATGATCCCAAGTCTTTGGGACCAATCTCTATTTTGATGTTTCCTCGCATGAACTACGCGTACACTTTGGTCTCAAATAGTACTAATAGCAGTTGCTCTAGTGGAAGTGAAGATACCCCACGCTCTTTACATGTTGAGAGATTTTGTTCACTACTATCATCAATAGTACTAAATCATGACTTTCCACTCAAATTTTCAAGCCACTGTATCACTCCAGAGAACTGCATTCCAATTTCTCTGTCTAATTTACCTCGGGCTGTGTTGTTTAAGGACATTGCATGCTCGGAGGATAATCGAAGGGTGAGAGTTCTGGTGGAATTTTCTGATGGTAGGCTAAACCAGTGCCCAACGCTCTTCAATCCTAATACAACTTTAGTTGGGGAAGGCTCATGGGATGCAAAGAAGAATCAGCTATGTGTTGTTGCTTGTCGATTCTTGAATACAACAAATTCTTTGAATAATACTAATGTGGGTGATTGTTCAACAAGATTGAGCTTGAGAATTCCCGCAACAGGGACAATTGGAAGTACTAGCAGCATTGTGGGGCATATTTGGAGCACCAAAACTGTGACAGAGTTGGGCTACTTTGAAAAGATCACATTTGCAAGTCGGGACTGTGGTTGTCGCAAGGGTCTACTTCCAGGCCAGACATATGAGTACACTAAAATGGAGAAAGTAACAAACTTGTGCTCGAGAAAGAAGACTGCAAATGATATGGCCAGCATTTACCCAAATCCATTTTCGCATGTCACGAGTTTCATTATGCATGCCGAAAATTCTGAAGGACAAGTTGGATTTGGCAATTGTGTTCCTCTCTCTGTTGACAATAGTATTTACCGGACTGATTGGTCTTCGGGGGAAGATGCATGTGCACCTGAAAGCAATCAAGAATACTCTGTGGCTCCTCTGACTTACAGCTACAACCACAGCAATCCCATCAATATTAGCTACAAAATAGATCTCCAACTACAATCTAATCCAAAGTGGGGCAAGCAGTCGGTTAAATATGAAATGCATATCAATGCTGAAGGGATCTATGATGGTACAGAAGGAAGCCTTTGCATGGTAGGCTGCCGCACACTTGTCTCAAACAGTCAGCAGGCAACAAATGATTCAATAGATTGTGAGATTCTTGTCCATTTTCAGTTCCCAGCAACAACTGCAAAGGATTTGCATCATATCAAAGGAAGCATTGAGAGCACTCGAAAGAAGTCCGATCCTCTCCATTTTGAACGCTTGGATTTGTCTACAGCTGCCAAGCATGTTGAAGAAAAGCGATCCATTTGGAGGATGGATGGGAAGATAACCTTGGTTCTTATATCAACCACACTTGCATGTGTTTTTGCTGCATTACAAATCTTCCATGTGAAAAGAAACCCTGAAGTCATTCCCTCTATTTCCATCTTAATGCTTCTAATTCTAACCCTTAGCTACATGTTACCTCTCGGGATTAATTTTGAAGCCATGTTCATGCATAATTTCAACCGCCAAAATGTGCACCTTGGAGGGGGTGGATGGCTTGAATTTAATGAGATAATCATAAGGGTGGCAACATTGGTAGCTTTCTTGATACAGTTCCACCTTCTGAAGCTAACTTGGTCAGCAAAATTGGGAAATGGAACCCGGAAGGATCTCCGGGTTATGGAAAAGAGGGCTCTCTTTGTGGCTTTACATGTGTATGCTGTAGGGGCTTTAGCTGCTTTGTTTCTGCATAGACAGAAACTGAGGAAGAGGGACGATCGCATTGAGTTGCCTCATCTAGAGAATACAGTTCTGGATGCCTTGACATCTTATGGTTGTTTGGTCTTGGATAGCTTTTTGTTCCCTCAAATTCTGCTCAACATATTCTGCCAATCGAAGGAAAATGCTCTATCAGTTTGGTTCTACATTGGAACAACTGTAGCTCGAGTTCTGCCACATGCACATGCTCTTTACAGGGCTCACAGCCCTGCCAATGACCAATTTGATGATTTCTCATACAATTATCCAGCTCCTGTGGCAGATTTATTCTCCATAGCTTGGAATGCCATCATTCCTTTAGAAGGTGTACTGTTTGCTGGTATCATATACTTGCAGCAGCGGTTTGGCGGTCGGTGCATTGCTCCTGGAAAGCTGCGAGAGCCTTATCCTGAGCCACAAAATTCAATTGAATTCAAACCCAGGCGTGTTGAAGAAACTGATGTACAAGGCTTATTCAAGGTTGAAGGAACTCTTAGATTTCCTAGAGCTAGTAGGAGGTACACCTATCTCAGAAGAGATTCCACAAGCCTGCCTCAATTTAACAGGTCTGCATCAAATCCTCCTCGACCAAGTTCAGTAAGCTTTTTCCTAACAGGGTTCTGGTCAGAATCTTCTGGGAAGCTTTGTATGGTGGGATCAGTCTGGTACCAGAGGCCTTTCAATCCCAATACCACCTTGGTTGGAGAAGGGTCATGGGATGCAAAGAAAACTCAGCTACAAGTTGTTGCTTGTCGATTTTTGAATGGAACAGACTCGTTGACAAATAGTATTCATGTGGGTGACGTTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
DUF2921

Protein Analysis

1034

Amino Acids

115.39

Weight (kDa)

8.15

Isoelectric Point (pI)

39.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2921_N PF25333 33 - 201 1.8e-16 DUF2921 lipocalin-like domain
DUF2921_N PF25333 308 - 391 1.9e-25 DUF2921 lipocalin-like domain
DUF2921_N PF25333 421 - 605 2.2e-32 DUF2921 lipocalin-like domain
DUF2921 PF11145 616 - 895 1.5e-54 Transmembrane E3 ligase/DUF2921 transmembrane domain
DUF2921_N PF25333 896 - 984 4.5e-10 DUF2921 lipocalin-like domain
DUF2921_N PF25333 986 - 1027 9.3e-06 DUF2921 lipocalin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21700
fragaria_vesca FvH4_1g10360 FvH4_1g10760 FvH4_2g30370 FvH4_3g09240 FvH4_3g20050 FvH4_3g20280 FvH4_3g20290 FvH4_3g20291 FvH4_3g20340 FvH4_3g20350 FvH4_3g20351 FvH4_3g20380 FvH4_3g20390 FvH4_5g13190 FvH4_6g20070 FvH4_6g42890
malus_domestica MD03G1227000.v1.1 MD10G1294900.v1.1 MD11G1246300.v1.1 MD11G1246400.v1.1 MD11G1246500.v1.1 MD11G1246600.v1.1
prunus_persica Prupe.4G047300_v2.0.a1 Prupe.4G181700_v2.0.a1 Prupe.4G181900_v2.0.a1 Prupe.4G182000_v2.0.a1 Prupe.4G182200_v2.0.a1
pyrus_communis pycom03g17470 pycom10g24670 pycom11g21640
rosa_chinensis RchiOBHm_Chr5g0008381 RchiOBHm_Chr5g0008391 RchiOBHm_Chr5g0033881 RchiOBHm_Chr5g0034171 RchiOBHm_Chr5g0034181 RchiOBHm_Chr5g0034191 RchiOBHm_Chr5g0034201 RchiOBHm_Chr5g0034211 RchiOBHm_Chr5g0034221 RchiOBHm_Chr5g0034231 RchiOBHm_Chr5g0034271 RchiOBHm_Chr5g0034281
rosa_laevigata RLG00000031566 RLG00000031567 RLG00000033506 RLG00000033529 RLG00000033530 RLG00000033531
rosa_multiflora Rmu_co8445509.1_g000001 Rmu_co8470615.1_g000001 Rmu_sc0008805.1_g000003 Rmu_sc0008805.1_g000004 Rmu_ssc0000128.1_g000029 Rmu_ssc0000128.1_g000031 Rmu_ssc0000128.1_g000032 Rmu_ssc0000388.1_g000062 Rmu_ssc0000388.1_g000063
rosa_roxburghii Rroxscaffold_1G00046010 Rroxscaffold_1G00046020 Rroxscaffold_1G00046030 Rroxscaffold_1G00046040 Rroxscaffold_1G00046060 Rroxscaffold_1G00046070 Rroxscaffold_1G00046080 Rroxscaffold_1G00046090
rosa_rugosa Rorug04G0435500 Rorug05G0142900 Rorug05G0144600 Rorug05G0144600 Rorug05G0144700 Rorug05G0144800
rosa_samantha Rh5AG233800 Rh5AG235900 Rh5AG236000 Rh5AG236100 Rh5AG236200 Rh5AG236300 Rh5AG236500 Rh5BG061200 Rh5BG061300 Rh5BG234300 Rh5BG236600 Rh5BG236800 Rh5CG071200 Rh5DG060000 Rh5DG060100 Rh5DG241300 Rh5DG243700 Rh5DG244000 Rh5DG244100
rosa_wichuraiana Rw3G018950 Rw5G005680 Rw5G021330 Rw5G021340 Rw5G021350 Rw5G021550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1420
Acc65I GGTACC 1 cut(s) 2958
AccB1I GGYRCC 1 cut(s) 2958
AccB7I CCANNNNNTGG 1 cut(s) 2592
AccI GTMKAC 2 cut(s) 1207, 1820
AccII CGCG 1 cut(s) 632
AciI CCGC 6 cut(s) 362, 1080, 1648, 2059, 2653, 2661
AclWI GGATC 8 cut(s) 160, 451, 572, 1613, 1784, 1845, 2211, 2956
AcoI YGGCCR 1 cut(s) 1290
AcuI CTGAAG 6 cut(s) 63, 99, 1374, 1620, 1974, 2180
AflII CTTAAG 1 cut(s) 488
AflIII ACRYGT 4 cut(s) 630, 703, 2007, 2245
AhlI ACTAGT 1 cut(s) 518
AjnI CCWGG 4 cut(s) 376, 1213, 2677, 2732
AjuI GAANNNNNNNTTGG 6 cut(s) 1297, 1329, 1347, 1379, 1865, 1897
Alw21I GWGCWC 5 cut(s) 1133, 1265, 1447, 1775, 2073
Alw26I GTCTC 2 cut(s) 649, 1663
Alw44I GTGCAC 2 cut(s) 1443, 2069
AlwI GGATC 8 cut(s) 160, 451, 572, 1613, 1784, 1845, 2211, 2956
AlwNI CAGNNNCTG 2 cut(s) 2296, 2567
Ama87I CYCGRG 5 cut(s) 125, 825, 1264, 2018, 2478
AoxI GGCC 3 cut(s) 1216, 1290, 2966
ApaLI GTGCAC 2 cut(s) 1443, 2069
ApeKI GCWGC 8 cut(s) 1107, 1644, 1826, 1919, 2270, 2647, 2650, 2686
AseI ATTAAT 1 cut(s) 2025
Asp700I GAANNNNTTC 3 cut(s) 20, 1959, 2830
Asp718I GGTACC 1 cut(s) 2958
AspS9I GGNCC 1 cut(s) 593
AsuC2I CCSGG 2 cut(s) 2197, 2210
AsuHPI GGTGA 3 cut(s) 886, 1058, 3104
AvaI CYCGRG 5 cut(s) 125, 825, 1264, 2018, 2478
AvaII GGWCC 1 cut(s) 593
BaeGI GKGCMC 3 cut(s) 920, 1447, 2073
BalI TGGCCA 1 cut(s) 1292
BanI GGYRCC 1 cut(s) 2958
BanII GRGCYC 2 cut(s) 2230, 2515
BarI GAAGNNNNNNTAC 2 cut(s) 670, 702
BauI CACGAG 1 cut(s) 1324
BbsI GAAGAC 2 cut(s) 1280, 1416
Bbv12I GWGCWC 5 cut(s) 1133, 1265, 1447, 1775, 2073
BbvI GCAGC 8 cut(s) 1119, 1631, 1813, 1906, 2257, 2659, 2662, 2673
BccI CCATC 9 cut(s) 14, 893, 1517, 1607, 1858, 1862, 1982, 2082, 2609
BciT130I CCWGG 4 cut(s) 378, 1215, 2679, 2734
BclI TGATCA 1 cut(s) 235
BcnI CCSGG 2 cut(s) 2197, 2210
BcoDI GTCTC 2 cut(s) 649, 1663
BcuI ACTAGT 1 cut(s) 518
BfaI CTAG 7 cut(s) 320, 519, 672, 1104, 2333, 2796, 2802
BfmI CTRYAG 3 cut(s) 1821, 2256, 2472
BfrI CTTAAG 1 cut(s) 488
BglII AGATCT 1 cut(s) 1531
BmcAI AGTACT 3 cut(s) 655, 741, 1102
Bme1390I CCNGG 6 cut(s) 378, 1215, 2197, 2210, 2679, 2734
Bme18I GGWCC 1 cut(s) 593
BmeT110I CYCGRG 5 cut(s) 125, 825, 1264, 2018, 2478
BmgT120I GGNCC 1 cut(s) 593
BmiI GGNNCC 5 cut(s) 544, 594, 1476, 2194, 2960
BmrFI CCNGG 6 cut(s) 378, 1215, 2197, 2210, 2679, 2734
BmsI GCATC 6 cut(s) 958, 1426, 1756, 2341, 2873, 3001
BpiI GAAGAC 2 cut(s) 1280, 1416
BpmI CTGGAG 1 cut(s) 771
Bpu10I CCTNAGC 2 cut(s) 2000, 2703
BpuEI CTTGAG 1 cut(s) 1090
BpuMI CCSGG 2 cut(s) 2197, 2210
BsaBI GATNNNNATC 1 cut(s) 1746
BsaI GGTCTC 1 cut(s) 649
BsaJI CCNNGG 5 cut(s) 824, 1881, 2074, 2732, 2988
BsaWI WCCGGW 1 cut(s) 1410
Bse1I ACTGG 2 cut(s) 199, 913
Bse3DI GCAATG 2 cut(s) 846, 2670
Bse8I GATNNNNATC 1 cut(s) 1746
BseBI CCWGG 4 cut(s) 378, 1215, 2679, 2734
BseDI CCNNGG 5 cut(s) 824, 1881, 2074, 2732, 2988
BseGI GGATG 6 cut(s) 973, 1869, 1873, 2093, 2356, 3016
BseJI GATNNNNATC 1 cut(s) 1746
BseMI GCAATG 2 cut(s) 846, 2670
BseMII CTCAG 6 cut(s) 170, 561, 2288, 2694, 2836, 3038
BseNI ACTGG 2 cut(s) 199, 913
BseRI GAGGAG 2 cut(s) 1467, 2865
BseSI GKGCMC 3 cut(s) 920, 1447, 2073
BseXI GCAGC 8 cut(s) 1119, 1631, 1813, 1906, 2257, 2659, 2662, 2673
BseYI CCCAGC 2 cut(s) 267, 1723
Bsh1236I CGCG 1 cut(s) 632
Bsh1285I CGRYCG 2 cut(s) 2314, 2665
BshFI GGCC 3 cut(s) 1218, 1292, 2968
BshNI GGYRCC 1 cut(s) 2958
BsiEI CGRYCG 2 cut(s) 2314, 2665
BsiHKAI GWGCWC 5 cut(s) 1133, 1265, 1447, 1775, 2073
BsiHKCI CYCGRG 5 cut(s) 125, 825, 1264, 2018, 2478
BsiSI CCGG 3 cut(s) 1411, 2197, 2209
BslFI GGGAC 4 cut(s) 606, 1102, 1199, 2321
BsmAI GTCTC 2 cut(s) 649, 1663
BsmFI GGGAC 4 cut(s) 606, 1102, 1199, 2321
BsmI GAATGC 2 cut(s) 798, 2602
BsnI GGCC 3 cut(s) 1218, 1292, 2968
Bso31I GGTCTC 1 cut(s) 649
BsoBI CYCGRG 5 cut(s) 125, 825, 1264, 2018, 2478
Bsp1286I GDGCHC 8 cut(s) 920, 1133, 1265, 1447, 1775, 2073, 2230, 2515
Bsp1407I TGTACA 3 cut(s) 163, 289, 2755
BspACI CCGC 6 cut(s) 362, 1080, 1648, 2059, 2653, 2661
BspANI GGCC 3 cut(s) 1218, 1292, 2968
BspCNI CTCAG 6 cut(s) 169, 562, 2289, 2695, 2835, 3037
BspFNI CGCG 1 cut(s) 632
BspHI TCATGA 3 cut(s) 507, 558, 748
BspLI GGNNCC 5 cut(s) 544, 594, 1476, 2194, 2960
BspPI GGATC 8 cut(s) 160, 451, 572, 1613, 1784, 1845, 2211, 2956
BspQI GCTCTTC 1 cut(s) 932
BspT107I GGYRCC 1 cut(s) 2958
BspTI CTTAAG 1 cut(s) 488
BspTNI GGTCTC 1 cut(s) 649
BsrDI GCAATG 2 cut(s) 846, 2670
BsrGI TGTACA 3 cut(s) 163, 289, 2755
BsrI ACTGG 2 cut(s) 199, 913
BssECI CCNNGG 5 cut(s) 824, 1881, 2074, 2732, 2988
BssSI CACGAG 1 cut(s) 1324
BssT1I CCWWGG 3 cut(s) 1881, 2074, 2988
Bst2BI CACGAG 1 cut(s) 1324
Bst2UI CCWGG 4 cut(s) 378, 1215, 2679, 2734
Bst4CI ACNGT 8 cut(s) 779, 1141, 1190, 1667, 2148, 2344, 2473, 2625
Bst6I CTCTTC 3 cut(s) 932, 2297, 2821
BstAFI CTTAAG 1 cut(s) 488
BstAPI GCANNNNNTGC 2 cut(s) 358, 1733
BstAUI TGTACA 3 cut(s) 163, 289, 2755
BstC8I GCNNGC 7 cut(s) 359, 853, 1294, 1341, 1565, 1674, 2843
BstDEI CTNAG 8 cut(s) 156, 570, 2000, 2297, 2703, 2786, 2822, 3024
BstENI CCTNNNNNAGG 1 cut(s) 2613
BstF5I GGATG 6 cut(s) 973, 1869, 1873, 2093, 2356, 3016
BstFNI CGCG 1 cut(s) 632
BstMAI GTCTC 2 cut(s) 649, 1663
BstMCI CGRYCG 2 cut(s) 2314, 2665
BstNI CCWGG 4 cut(s) 378, 1215, 2679, 2734
BstSCI CCNGG 6 cut(s) 376, 1213, 2195, 2208, 2677, 2732
BstSFI CTRYAG 3 cut(s) 1821, 2256, 2472
BstSLI GKGCMC 3 cut(s) 920, 1447, 2073
BstUI CGCG 1 cut(s) 632
BstV1I GCAGC 8 cut(s) 1119, 1631, 1813, 1906, 2257, 2659, 2662, 2673
BstV2I GAAGAC 2 cut(s) 1280, 1416
BstX2I RGATCY 4 cut(s) 152, 1531, 1605, 2203
BstXI CCANNNNNNTGG 1 cut(s) 589
BstYI RGATCY 4 cut(s) 152, 1531, 1605, 2203
BsuRI GGCC 3 cut(s) 1218, 1292, 2968
BtsCI GGATG 6 cut(s) 973, 1869, 1873, 2093, 2356, 3016
BtsI GCAGTG 1 cut(s) 211
BtsIMutI CAGTG 4 cut(s) 192, 211, 775, 920
Cac8I GCNNGC 7 cut(s) 359, 853, 1294, 1341, 1565, 1674, 2843
CaiI CAGNNNCTG 2 cut(s) 2296, 2567
CciI TCATGA 3 cut(s) 507, 558, 748
Cfr13I GGNCC 1 cut(s) 593
CspCI CAANNNNNGTGG 2 cut(s) 2974, 3009
DdeI CTNAG 8 cut(s) 156, 570, 2000, 2297, 2703, 2786, 2822, 3024
DrdI GACNNNNNNGTC 1 cut(s) 1420
DseDI GACNNNNNNGTC 1 cut(s) 1420
EaeI YGGCCR 1 cut(s) 1290
Eam1104I CTCTTC 3 cut(s) 932, 2297, 2821
EarI CTCTTC 3 cut(s) 932, 2297, 2821
Eco130I CCWWGG 3 cut(s) 1881, 2074, 2988
Eco147I AGGCCT 1 cut(s) 2968
Eco24I GRGCYC 2 cut(s) 2230, 2515
Eco31I GGTCTC 1 cut(s) 649
Eco32I GATATC 1 cut(s) 567
Eco47I GGWCC 1 cut(s) 593
Eco57I CTGAAG 6 cut(s) 63, 99, 1374, 1620, 1974, 2180
Eco88I CYCGRG 5 cut(s) 125, 825, 1264, 2018, 2478
EcoNI CCTNNNNNAGG 1 cut(s) 2613
EcoRI GAATTC 2 cut(s) 1074, 2723
EcoRII CCWGG 4 cut(s) 376, 1213, 2677, 2732
EcoRV GATATC 1 cut(s) 567
EcoT14I CCWWGG 3 cut(s) 1881, 2074, 2988
EcoT22I ATGCAT 4 cut(s) 1341, 1441, 1590, 2049
EcoT38I GRGCYC 2 cut(s) 2230, 2515
ErhI CCWWGG 3 cut(s) 1881, 2074, 2988
FalI AAGNNNNNCTT 6 cut(s) 1614, 1646, 1865, 1897, 2769, 2801
FaqI GGGAC 4 cut(s) 606, 1102, 1199, 2321
FauI CCCGC 1 cut(s) 1087
FauNDI CATATG 1 cut(s) 1225
FbaI TGATCA 1 cut(s) 235
FblI GTMKAC 2 cut(s) 1207, 1820
FokI GGATG 6 cut(s) 980, 1876, 1880, 2100, 2363, 3023
FriOI GRGCYC 2 cut(s) 2230, 2515
FspBI CTAG 7 cut(s) 320, 519, 672, 1104, 2333, 2796, 2802
GsaI CCCAGC 2 cut(s) 271, 1727
GsuI CTGGAG 1 cut(s) 771
HaeIII GGCC 3 cut(s) 1218, 1292, 2968
HapII CCGG 3 cut(s) 1411, 2197, 2209
HincII GTYRAC 3 cut(s) 132, 1396, 3072
HindII GTYRAC 3 cut(s) 132, 1396, 3072
HindIII AAGCTT 3 cut(s) 17, 2893, 2933
HpaII CCGG 3 cut(s) 1411, 2197, 2209
HphI GGTGA 3 cut(s) 886, 1058, 3104
HpyCH4III ACNGT 8 cut(s) 779, 1141, 1190, 1667, 2148, 2344, 2473, 2625
HpyCH4IV ACGT 1 cut(s) 3096
HpyF3I CTNAG 8 cut(s) 156, 570, 2000, 2297, 2703, 2786, 2822, 3024
HpySE526I ACGT 1 cut(s) 3096
KpnI GGTACC 1 cut(s) 2962
Ksp22I TGATCA 1 cut(s) 235
LguI GCTCTTC 1 cut(s) 932
LmnI GCTCC 4 cut(s) 1128, 1480, 2569, 2680
Lsp1109I GCAGC 8 cut(s) 1119, 1631, 1813, 1906, 2257, 2659, 2662, 2673
LweI GCATC 6 cut(s) 958, 1426, 1756, 2341, 2873, 3001
MaeI CTAG 7 cut(s) 320, 519, 672, 1104, 2333, 2796, 2802
MaeII ACGT 1 cut(s) 3096
MaeIII GTNAC 8 cut(s) 206, 331, 523, 1141, 1249, 1321, 2010, 3092
MfeI CAATTG 3 cut(s) 1092, 1375, 2718
MflI RGATCY 4 cut(s) 152, 1531, 1605, 2203
MhlI GDGCHC 8 cut(s) 920, 1133, 1265, 1447, 1775, 2073, 2230, 2515
MlsI TGGCCA 1 cut(s) 1292
MluI ACGCGT 1 cut(s) 630
MluNI TGGCCA 1 cut(s) 1292
MlyI GAGTC 3 cut(s) 137, 390, 3059
MmeI TCCRAC 4 cut(s) 421, 1345, 1561, 2974
Mox20I TGGCCA 1 cut(s) 1292
Mph1103I ATGCAT 4 cut(s) 1341, 1441, 1590, 2049
MroXI GAANNNNTTC 3 cut(s) 20, 1959, 2830
MscI TGGCCA 1 cut(s) 1292
MseI TTAA 9 cut(s) 216, 303, 489, 840, 1575, 1979, 2025, 2100, 2853
Msp20I TGGCCA 1 cut(s) 1292
MspA1I CMGCKG 2 cut(s) 1826, 2653
MspCI CTTAAG 1 cut(s) 488
MspI CCGG 3 cut(s) 1411, 2197, 2209
MspR9I CCNGG 6 cut(s) 378, 1215, 2197, 2210, 2679, 2734
MunI CAATTG 3 cut(s) 1092, 1375, 2718
Mva1269I GAATGC 2 cut(s) 798, 2602
MvaI CCWGG 4 cut(s) 378, 1215, 2679, 2734
MvnI CGCG 1 cut(s) 632
NciI CCSGG 2 cut(s) 2197, 2210
NdeI CATATG 1 cut(s) 1225
NlaIV GGNNCC 5 cut(s) 544, 594, 1476, 2194, 2960
NmuCI GTSAC 4 cut(s) 206, 1141, 1321, 3092
NsiI ATGCAT 4 cut(s) 1341, 1441, 1590, 2049
PaeI GCATGC 3 cut(s) 361, 855, 1343
PaeR7I CTCGAG 2 cut(s) 1264, 2478
PagI TCATGA 3 cut(s) 507, 558, 748
PceI AGGCCT 1 cut(s) 2968
PciI ACATGT 3 cut(s) 703, 2007, 2245
PciSI GCTCTTC 1 cut(s) 932
PcsI WCGNNNNNNNCGW 1 cut(s) 1322
PctI GAATGC 2 cut(s) 798, 2602
PdmI GAANNNNTTC 3 cut(s) 20, 1959, 2830
PfeI GAWTC 8 cut(s) 172, 405, 979, 1005, 1685, 1702, 2831, 2921
PflMI CCANNNNNTGG 1 cut(s) 2592
PfoI TCCNGGA 1 cut(s) 2677
Ple19I CGATCG 1 cut(s) 2314
PleI GAGTC 3 cut(s) 136, 390, 3059
PpsI GAGTC 3 cut(s) 136, 390, 3059
PscI ACATGT 3 cut(s) 703, 2007, 2245
PshBI ATTAAT 1 cut(s) 2025
Psp6I CCWGG 4 cut(s) 376, 1213, 2677, 2732
PspFI CCCAGC 2 cut(s) 267, 1723
PspGI CCWGG 4 cut(s) 376, 1213, 2677, 2732
PspN4I GGNNCC 5 cut(s) 544, 594, 1476, 2194, 2960
PspPI GGNCC 1 cut(s) 593
PspXI VCTCGAGB 1 cut(s) 2478
PsrI GAACNNNNNNTAC 4 cut(s) 617, 649, 2466, 2498
PstNI CAGNNNCTG 2 cut(s) 2296, 2567
PsuI RGATCY 4 cut(s) 152, 1531, 1605, 2203
PvuI CGATCG 1 cut(s) 2314
PvuII CAGCTG 1 cut(s) 1826
SapI GCTCTTC 1 cut(s) 932
SaqAI TTAA 9 cut(s) 216, 303, 489, 840, 1575, 1979, 2025, 2100, 2853
Sau96I GGNCC 1 cut(s) 593
ScaI AGTACT 3 cut(s) 655, 741, 1102
SchI GAGTC 3 cut(s) 137, 390, 3059
ScrFI CCNGG 6 cut(s) 378, 1215, 2197, 2210, 2679, 2734
SduI GDGCHC 8 cut(s) 920, 1133, 1265, 1447, 1775, 2073, 2230, 2515
SfaNI GCATC 6 cut(s) 958, 1426, 1756, 2341, 2873, 3001
SfcI CTRYAG 3 cut(s) 1821, 2256, 2472
Sfr274I CTCGAG 2 cut(s) 1264, 2478
SinI GGWCC 1 cut(s) 593
SlaI CTCGAG 2 cut(s) 1264, 2478
SmlI CTYRAG 4 cut(s) 488, 1069, 1264, 2478
SmoI CTYRAG 4 cut(s) 488, 1069, 1264, 2478
SpeI ACTAGT 1 cut(s) 518
SphI GCATGC 3 cut(s) 361, 855, 1343
SseBI AGGCCT 1 cut(s) 2968
SsiI CCGC 6 cut(s) 362, 1080, 1648, 2059, 2653, 2661
SspI AATATT 1 cut(s) 1516
SspMI CTAG 7 cut(s) 320, 519, 672, 1104, 2333, 2796, 2802
StuI AGGCCT 1 cut(s) 2968
StyD4I CCNGG 6 cut(s) 376, 1213, 2195, 2208, 2677, 2732
StyI CCWWGG 3 cut(s) 1881, 2074, 2988
TaaI ACNGT 8 cut(s) 779, 1141, 1190, 1667, 2148, 2344, 2473, 2625
TaiI ACGT 1 cut(s) 3099
TatI WGTACW 8 cut(s) 163, 289, 653, 739, 1100, 1230, 2620, 2755
TauI GCSGC 2 cut(s) 364, 1650
TfiI GAWTC 8 cut(s) 172, 405, 979, 1005, 1685, 1702, 2831, 2921
Tru1I TTAA 9 cut(s) 216, 303, 489, 840, 1575, 1979, 2025, 2100, 2853
Tru9I TTAA 9 cut(s) 216, 303, 489, 840, 1575, 1979, 2025, 2100, 2853
TscAI CASTG 4 cut(s) 199, 211, 782, 920
TseFI GTSAC 4 cut(s) 206, 1141, 1321, 3092
TseI GCWGC 8 cut(s) 1107, 1644, 1826, 1919, 2270, 2647, 2650, 2686
Tsp45I GTSAC 4 cut(s) 206, 1141, 1321, 3092
TspRI CASTG 4 cut(s) 199, 211, 782, 920
Van91I CCANNNNNTGG 1 cut(s) 2592
Vha464I CTTAAG 1 cut(s) 488
VneI GTGCAC 2 cut(s) 1443, 2069
VpaK11BI GGWCC 1 cut(s) 593
VspI ATTAAT 1 cut(s) 2025
XagI CCTNNNNNAGG 1 cut(s) 2613
XbaI TCTAGA 2 cut(s) 319, 2332
XcmI CCANNNNNNNNNTGG 2 cut(s) 1861, 2986
XhoI CTCGAG 2 cut(s) 1264, 2478
XmiI GTMKAC 2 cut(s) 1207, 1820
XmnI GAANNNNTTC 3 cut(s) 20, 1959, 2830
XspI CTAG 7 cut(s) 320, 519, 672, 1104, 2333, 2796, 2802
ZrmI AGTACT 3 cut(s) 655, 741, 1102
Zsp2I ATGCAT 4 cut(s) 1341, 1441, 1590, 2049
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.