Rh5AG236000

Protein of unknown function (DUF2921)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000420
Physical Location & Seq
Reverse (-)
28654584 .. 28655228
645 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG236000.1

Sequence Viewer

Length: 645 bp
ATGTGCATGGTAGGCTGCCGAAATCTAGGCCAGATAGATCAACAGCCAACTGATGATTCTGTAGATTGTGAGATTCTACTCAAACTTCAGTTGCCTCCAGCAACTCCAGGGAGGAAGATCTCAGGTTATGTCAAGGGGAGCATTGAAAGCATACGGAAAAAGTCTGATCCTCTTCATTTTGAACGTTTAGACTTGTCTTCAGATGACATGTTTATGGCTGAAGCAGATCGATCCATTTGTAGGTTGGAGGTGGAGACCACCTTGGTTCTTATATCCACCACACTTGCATATGTTTTTGGGGCATTACAACTCTTCCATATGAACAAACGTCCAGATGTGCTTCCCTCCATTTCAATCTTGATGCTGCTAGTTCTAACCCTTGGGTTTTTGAAACCTCTTATATATTACTTTGAAGCCATCCTCACCTATAGCACCGATTACCAGGATGTGTTCATTGGAAGTGATGGATGGCTTCAAGTTGATCAGGCAAATCTGAGGGCAATAGCAGTTATGATAATGCTAGCTTTCTTGTTGCACTTCAACCTTTTGCAGCAAACATGGTCAGCAAGATCTGCGAAAGGGACCTTGAAGGACCTTTGGAATGAGAGAAGAAAGCTTTGTCAGTATATGCAGTTGGGTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
DUF2921

Protein Analysis

214

Amino Acids

24.36

Weight (kDa)

5.92

Isoelectric Point (pI)

41.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2921_N PF25333 1 - 64 4.6e-18 DUF2921 lipocalin-like domain
DUF2921 PF11145 76 - 206 5.1e-20 Transmembrane E3 ligase/DUF2921 transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21700
fragaria_vesca FvH4_1g10360 FvH4_1g10760 FvH4_2g30370 FvH4_3g09240 FvH4_3g20050 FvH4_3g20280 FvH4_3g20290 FvH4_3g20291 FvH4_3g20340 FvH4_3g20350 FvH4_3g20351 FvH4_3g20380 FvH4_3g20390 FvH4_5g13190 FvH4_6g20070 FvH4_6g42890
malus_domestica MD03G1227000.v1.1 MD10G1294900.v1.1 MD11G1246300.v1.1 MD11G1246400.v1.1 MD11G1246500.v1.1 MD11G1246600.v1.1
prunus_persica Prupe.4G047300_v2.0.a1 Prupe.4G181700_v2.0.a1 Prupe.4G181900_v2.0.a1 Prupe.4G182000_v2.0.a1 Prupe.4G182200_v2.0.a1
pyrus_communis pycom03g17470 pycom10g24670 pycom11g21640
rosa_chinensis RchiOBHm_Chr5g0008381 RchiOBHm_Chr5g0008391 RchiOBHm_Chr5g0033881 RchiOBHm_Chr5g0034171 RchiOBHm_Chr5g0034181 RchiOBHm_Chr5g0034191 RchiOBHm_Chr5g0034201 RchiOBHm_Chr5g0034211 RchiOBHm_Chr5g0034221 RchiOBHm_Chr5g0034231 RchiOBHm_Chr5g0034271 RchiOBHm_Chr5g0034281
rosa_laevigata RLG00000031566 RLG00000031567 RLG00000033506 RLG00000033529 RLG00000033530 RLG00000033531
rosa_multiflora Rmu_co8445509.1_g000001 Rmu_co8470615.1_g000001 Rmu_sc0008805.1_g000003 Rmu_sc0008805.1_g000004 Rmu_ssc0000128.1_g000029 Rmu_ssc0000128.1_g000031 Rmu_ssc0000128.1_g000032 Rmu_ssc0000388.1_g000062 Rmu_ssc0000388.1_g000063
rosa_roxburghii Rroxscaffold_1G00046010 Rroxscaffold_1G00046020 Rroxscaffold_1G00046030 Rroxscaffold_1G00046040 Rroxscaffold_1G00046060 Rroxscaffold_1G00046070 Rroxscaffold_1G00046080 Rroxscaffold_1G00046090
rosa_rugosa Rorug04G0435500 Rorug05G0142900 Rorug05G0144600 Rorug05G0144600 Rorug05G0144700 Rorug05G0144800
rosa_samantha Rh5AG233800 Rh5AG235900 Rh5AG236000 Rh5AG236100 Rh5AG236200 Rh5AG236300 Rh5AG236500 Rh5BG061200 Rh5BG061300 Rh5BG234300 Rh5BG236600 Rh5BG236800 Rh5CG071200 Rh5DG060000 Rh5DG060100 Rh5DG241300 Rh5DG243700 Rh5DG244000 Rh5DG244100
rosa_wichuraiana Rw3G018950 Rw5G005680 Rw5G021330 Rw5G021340 Rw5G021350 Rw5G021550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 184
AclWI GGATC 2 cut(s) 161, 225
AcuI CTGAAG 3 cut(s) 71, 183, 240
AfiI CCNNNNNNNGG 1 cut(s) 240
AflIII ACRYGT 1 cut(s) 207
AgsI TTSAA 8 cut(s) 146, 182, 354, 391, 413, 476, 541, 589
AjnI CCWGG 2 cut(s) 106, 441
AjuI GAANNNNNNNTTGG 2 cut(s) 40, 72
AluBI AGCT 2 cut(s) 524, 616
AluI AGCT 2 cut(s) 524, 616
Alw26I GTCTC 1 cut(s) 248
AlwI GGATC 2 cut(s) 161, 225
AoxI GGCC 1 cut(s) 28
ApeKI GCWGC 3 cut(s) 15, 364, 550
AspS9I GGNCC 2 cut(s) 582, 592
AsuHPI GGTGA 1 cut(s) 415
AsuNHI GCTAGC 1 cut(s) 520
AvaII GGWCC 2 cut(s) 582, 592
BbsI GAAGAC 1 cut(s) 189
BbvI GCAGC 3 cut(s) 2, 351, 562
BccI CCATC 3 cut(s) 425, 458, 462
BciT130I CCWGG 2 cut(s) 108, 443
BclI TGATCA 1 cut(s) 481
BcoDI GTCTC 1 cut(s) 248
BfaI CTAG 3 cut(s) 26, 368, 521
BfmI CTRYAG 2 cut(s) 60, 427
BglII AGATCT 2 cut(s) 117, 569
BisI GCNGC 3 cut(s) 16, 365, 551
BlsI GCNGC 3 cut(s) 17, 366, 552
Bme1390I CCNGG 2 cut(s) 108, 443
Bme18I GGWCC 2 cut(s) 582, 592
BmgT120I GGNCC 2 cut(s) 582, 592
BmiI GGNNCC 1 cut(s) 583
BmrFI CCNGG 2 cut(s) 108, 443
BmsI GCATC 1 cut(s) 351
BmtI GCTAGC 1 cut(s) 524
BpiI GAAGAC 1 cut(s) 189
BpmI CTGGAG 2 cut(s) 81, 90
Bsa29I ATCGAT 1 cut(s) 229
BsaI GGTCTC 1 cut(s) 248
BsaJI CCNNGG 3 cut(s) 107, 261, 379
Bsc4I CCNNNNNNNGG 1 cut(s) 240
BseBI CCWGG 2 cut(s) 108, 443
BseCI ATCGAT 1 cut(s) 229
BseDI CCNNGG 3 cut(s) 107, 261, 379
BseGI GGATG 3 cut(s) 417, 451, 473
BseLI CCNNNNNNNGG 1 cut(s) 240
BseMII CTCAG 2 cut(s) 135, 485
BseXI GCAGC 3 cut(s) 2, 351, 562
BshFI GGCC 1 cut(s) 30
BshVI ATCGAT 1 cut(s) 229
BslFI GGGAC 1 cut(s) 595
BslI CCNNNNNNNGG 1 cut(s) 240
BsmAI GTCTC 1 cut(s) 248
BsmFI GGGAC 1 cut(s) 595
BsnI GGCC 1 cut(s) 30
Bso31I GGTCTC 1 cut(s) 248
Bsp143I GATC 7 cut(s) 37, 117, 166, 226, 230, 481, 569
BspANI GGCC 1 cut(s) 30
BspCNI CTCAG 2 cut(s) 134, 486
BspDI ATCGAT 1 cut(s) 229
BspLI GGNNCC 1 cut(s) 583
BspOI GCTAGC 1 cut(s) 524
BspPI GGATC 2 cut(s) 161, 225
BspTNI GGTCTC 1 cut(s) 248
BssECI CCNNGG 3 cut(s) 107, 261, 379
BssMI GATC 7 cut(s) 37, 117, 166, 226, 230, 481, 569
BssT1I CCWWGG 2 cut(s) 261, 379
Bst2UI CCWGG 2 cut(s) 108, 443
Bst6I CTCTTC 2 cut(s) 177, 317
BstAPI GCANNNNNTGC 1 cut(s) 572
BstC8I GCNNGC 1 cut(s) 522
BstDEI CTNAG 2 cut(s) 121, 494
BstF5I GGATG 3 cut(s) 417, 451, 473
BstKTI GATC 7 cut(s) 40, 120, 169, 229, 233, 484, 572
BstMAI GTCTC 1 cut(s) 248
BstMBI GATC 7 cut(s) 37, 117, 166, 226, 230, 481, 569
BstMWI GCNNNNNNNGC 3 cut(s) 12, 147, 572
BstNI CCWGG 2 cut(s) 108, 443
BstNSI RCATGY 1 cut(s) 211
BstSCI CCNGG 2 cut(s) 106, 441
BstSFI CTRYAG 2 cut(s) 60, 427
BstV1I GCAGC 3 cut(s) 2, 351, 562
BstV2I GAAGAC 1 cut(s) 189
BstX2I RGATCY 2 cut(s) 117, 569
BstYI RGATCY 2 cut(s) 117, 569
Bsu15I ATCGAT 1 cut(s) 229
BsuRI GGCC 1 cut(s) 30
BsuTUI ATCGAT 1 cut(s) 229
BtsCI GGATG 3 cut(s) 417, 451, 473
Cac8I GCNNGC 1 cut(s) 522
Cfr13I GGNCC 2 cut(s) 582, 592
ClaI ATCGAT 1 cut(s) 229
CspCI CAANNNNNGTGG 2 cut(s) 265, 300
CviAII CATG 3 cut(s) 7, 208, 558
CviJI RGCY 8 cut(s) 15, 30, 46, 218, 416, 472, 524, 616
CviKI_1 RGCY 8 cut(s) 15, 30, 46, 218, 416, 472, 524, 616
DdeI CTNAG 2 cut(s) 121, 494
DpnI GATC 7 cut(s) 39, 119, 168, 228, 232, 483, 571
DpnII GATC 7 cut(s) 37, 117, 166, 226, 230, 481, 569
Eam1104I CTCTTC 2 cut(s) 177, 317
EarI CTCTTC 2 cut(s) 177, 317
Eco130I CCWWGG 2 cut(s) 261, 379
Eco31I GGTCTC 1 cut(s) 248
Eco47I GGWCC 2 cut(s) 582, 592
Eco57I CTGAAG 3 cut(s) 71, 183, 240
EcoO109I RGGNCCY 2 cut(s) 582, 592
EcoRII CCWGG 2 cut(s) 106, 441
EcoT14I CCWWGG 2 cut(s) 261, 379
ErhI CCWWGG 2 cut(s) 261, 379
FaeI CATG 3 cut(s) 10, 211, 561
FaqI GGGAC 1 cut(s) 595
FatI CATG 3 cut(s) 6, 207, 557
FauNDI CATATG 2 cut(s) 289, 318
FbaI TGATCA 1 cut(s) 481
Fnu4HI GCNGC 3 cut(s) 16, 365, 551
FokI GGATG 3 cut(s) 404, 458, 480
Fsp4HI GCNGC 3 cut(s) 16, 365, 551
FspBI CTAG 3 cut(s) 26, 368, 521
GluI GCNGC 3 cut(s) 16, 365, 551
GsuI CTGGAG 2 cut(s) 81, 90
HaeIII GGCC 1 cut(s) 30
Hin1II CATG 3 cut(s) 10, 211, 561
HindIII AAGCTT 1 cut(s) 614
HinfI GANTC 2 cut(s) 56, 73
HphI GGTGA 1 cut(s) 415
Hpy188I TCNGA 3 cut(s) 166, 202, 495
Hpy188III TCNNGA 2 cut(s) 332, 358
HpyAV CCTTC 1 cut(s) 583
HpyCH4IV ACGT 2 cut(s) 184, 328
HpyCH4V TGCA 5 cut(s) 6, 287, 535, 550, 631
HpyF10VI GCNNNNNNNGC 3 cut(s) 12, 147, 572
HpyF3I CTNAG 2 cut(s) 121, 494
HpySE526I ACGT 2 cut(s) 184, 328
Hsp92II CATG 3 cut(s) 10, 211, 561
Ksp22I TGATCA 1 cut(s) 481
Kzo9I GATC 7 cut(s) 37, 117, 166, 226, 230, 481, 569
LmnI GCTCC 1 cut(s) 138
LpnPI CCDG 9 cut(s) 44, 93, 108, 111, 120, 345, 428, 455, 470
Lsp1109I GCAGC 3 cut(s) 2, 351, 562
LweI GCATC 1 cut(s) 351
MaeI CTAG 3 cut(s) 26, 368, 521
MaeII ACGT 2 cut(s) 184, 328
MalI GATC 7 cut(s) 39, 119, 168, 228, 232, 483, 571
MboI GATC 7 cut(s) 37, 117, 166, 226, 230, 481, 569
MboII GAAGA 5 cut(s) 127, 164, 189, 304, 621
MflI RGATCY 2 cut(s) 117, 569
MmeI TCCRAC 1 cut(s) 225
MnlI CCTC 8 cut(s) 105, 105, 180, 241, 355, 405, 431, 489
MslI CAYNNNNRTG 1 cut(s) 212
MspR9I CCNGG 2 cut(s) 108, 443
MvaI CCWGG 2 cut(s) 108, 443
MwoI GCNNNNNNNGC 3 cut(s) 12, 147, 572
NdeI CATATG 2 cut(s) 289, 318
NdeII GATC 7 cut(s) 37, 117, 166, 226, 230, 481, 569
NheI GCTAGC 1 cut(s) 520
NlaIII CATG 3 cut(s) 10, 211, 561
NlaIV GGNNCC 1 cut(s) 583
NspI RCATGY 1 cut(s) 211
PciI ACATGT 1 cut(s) 207
PfeI GAWTC 2 cut(s) 56, 73
PkrI GCNGC 3 cut(s) 17, 366, 552
PpuMI RGGWCCY 2 cut(s) 582, 592
PscI ACATGT 1 cut(s) 207
Psp1406I AACGTT 1 cut(s) 184
Psp5II RGGWCCY 2 cut(s) 582, 592
Psp6I CCWGG 2 cut(s) 106, 441
PspGI CCWGG 2 cut(s) 106, 441
PspN4I GGNNCC 1 cut(s) 583
PspPI GGNCC 2 cut(s) 582, 592
PspPPI RGGWCCY 2 cut(s) 582, 592
PsuI RGATCY 2 cut(s) 117, 569
RseI CAYNNNNRTG 1 cut(s) 212
SatI GCNGC 3 cut(s) 16, 365, 551
Sau3AI GATC 7 cut(s) 37, 117, 166, 226, 230, 481, 569
Sau96I GGNCC 2 cut(s) 582, 592
ScrFI CCNGG 2 cut(s) 108, 443
SfaNI GCATC 1 cut(s) 351
SfcI CTRYAG 2 cut(s) 60, 427
SinI GGWCC 2 cut(s) 582, 592
SmiMI CAYNNNNRTG 1 cut(s) 212
SspMI CTAG 3 cut(s) 26, 368, 521
StyD4I CCNGG 2 cut(s) 106, 441
StyI CCWWGG 2 cut(s) 261, 379
TaiI ACGT 2 cut(s) 187, 331
TaqI TCGA 1 cut(s) 229
TfiI GAWTC 2 cut(s) 56, 73
TseI GCWGC 3 cut(s) 15, 364, 550
TspDTI ATGAA 3 cut(s) 164, 335, 442
TspGWI ACGGA 1 cut(s) 169
VpaK11BI GGWCC 2 cut(s) 582, 592
XceI RCATGY 1 cut(s) 211
XcmI CCANNNNNNNNNTGG 1 cut(s) 241
XspI CTAG 3 cut(s) 26, 368, 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.