MD11G1246400.v1.1

Protein of unknown function (DUF2921)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000420
Physical Location & Seq
Reverse (-)
35822127 .. 35824964
2838 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1246400.v1.1.491

Sequence Viewer

Length: 2838 bp
ATGTTCATGAAGAAACACCTGTCTTCTGCTACCACCTCTTTGAAGATGTGGTACTCATTGCCAAAGCTTCTCATGTTCTTCTTTGTTTTTGCTGCCTTTTCTTTTCCCTCCATTTCATCGCCTTATCCACCACCTTCTAATCACTTCTCTTATCCAGTTTCATCGCCTTCTCCACCTTATGATCAAAGCTCTTATTCTGATCACTGTGATTCAATTGTTCGCTGGTCAAAACCAAGAACATATGTAGCTTCTCAAATCCTCAACCGCCATACAGGTTACTACACTGGTGGAAGTGGAATTCTTGGTCAAAAATCATCATTTCCGCCTTTTCACGAGCCTGAAGATTCAATTCAGTTCAACTTCTGGAGTGTTCAAAAAACTTATGTACCAGGCTTGTTCATGATTGAAGGAAGTCTTCTATTTCCAAGAAGCAGTGTGTCTTACTATGTGGGAAATGTCTCAAAAAGTCATCGACCAAGTTCGGTAAGCTTTAAACTAAGTGGATTCTGGTCAGAATCTTCTGGGAAGATTTGCATGGTTGGATCAAGTTCTAATTACTTGGGACATGGTCGTTGGCTTTATTATTCTGCTGTTCTTAAGCTTTATAATGTCATGAATTCCACTAATGTTACTAGTTTGATTAGTGGAACCTTAGAGAGCCCGATGAAATCTAAGAGCAATCCACAGTATTTTGAACCAGTCTCGATTTTGATAATTCCTCGTATAAATTACGAGTACTCTTTGGTGTCAAATAAATCTGATAACAGTTGTTCTGGTGGAAGAAATATTCACCCAAGTTCTTTGCCGATAGAGACAGTTTGTCCAGTGCTTTCAAGAGTAAGAAGACATGAGTTTGAGCTCAAATACTCAAGTCATTGCCGTTCTGCAAAGAACTGCTCTCCGGTTGCTGCTTCTGATTTACCTCACATTTTGTCGTTGAAGGCTATTGAGTGCTCGGAGAACACAAAAAGGATGAGAGTTCAAGTTGAATTTGGAGACAGTAGCAATCCCTGGTACCGGAGGCCCTTTTATCCCAATACAACATTGGTTGGGGAAGCATCATGGGATGCAAAGAACAATCAGCTATGTGTTGTTGCTTGTCGAATCTTGAATGCAACAGATTCTATCACTAATAGTACTCATGTGGATGATTGTTCAACAAGATTGAGCTTGAGGTTTCCTGCAATATGGACGATCGGAAAGACAAGTAGCACTGTGGGCCATATTTGGAGCAACAAAAATGCGACAGAGTTGGGTTACTTTGAAAGCATCACATTTGAAAGTCCTCAGAATTATATTGGAAGGTTTCTACCTCCAGGCTTGAAATATGAGTATACTAAACTTGACAAAGTAACCAAGATGTGCCCGAGAAAGAAGGGTGCTCATCGCAAGACCTACAAATATCCAAAACCGCTTTCTTATGACATGCGATTTGGCATGCAAGTCAAATACAAAGGGGAGGTTGCATGGGGCAGTGCTGCTCCTGTCTCCATCGGAAACAGGTTTTACCATCGTTATGGATATTTAAATGGTGCAAGCAGGGAACGCTTTGTACCTCCTGTGAGCTACAGCTACAACGACATCCCTGTCAACATCAGCTACCAAATAAGCATCAATTTCAATCAGTTGGGAAATGTAATTTCTAAACTGAAAAGATCTTCAAATTCAAGTGAAGTGAAAATTTATGCTGAAGGGATCTACGATCCTACTGATGGATGCCTGTGTATGGTAGGATGCAGAAATCTGGGCCCAGACAGTCAACAAGAAGCAGATGATTCTGTAGATTGTGAGATTCTTGTCAAATTTCAGTTCCCTCCAACAAACAAAAAGAATGCAGATGTTATCAAGGGCAGCATTGAAAGCACACGGAAAGAGTCCGATCCTCTTCATTTTGGAAGTTGGGGTTTGTCTTCAGATTCTATTACTGTAGTTGTAGCAGAGCGATCCATTTGGAGGATGGATGCAGAGATTACCTTGGTTTTGATATCTACCACACTTGCATGTGTATTTGTGGCATTACAACTCTTCCATGTGAAAAAACATCCCGACGTGCTTCCCTCCATTTCCACTTTCATGCTACTAATTCTAAGTCTGGGCTACATGATACCTCTAATGCTAAACTTGAAAGCCATGTTCGTGAATAACACCAACGTTCAACATGTGCTCCTTGGAGGCGGTGGATGGCTTGAAGTTAACGGGGAAATTGTAAGAGCAGTAACAATGGTAGCTTTTTTGTTGCAAATCCGTCTTCTGCAGCTAACATTGGCAGAAAGATCACAAAATGAAACCCGAAAGGAGCTCTGGCTAAAGGAAAGGAAGTCTCTCTTTGTGGTTTTACCTGTATATGCTGCAGGGGCTTTAGCTGCTTTCCTTCTGCATAGACAGAAATGGACCAAAAGCCACAAAATTGGCGCCGCAACTTCCTATCCAGACCATTCAATTTTGGGTGCTGCCTTGAAGTCTTATGCCGGTTTAGTGATGGATGGTTTTCTGCTACCTCAGATACTGCTCAACATGTTCTGTGAATCGAAGGAAAAGGTTCTGTCTGTTTGGTTCTACATCGGAACAACTTTTGTTCGAGGTCTGCCGCATGCATATGATCTGTACAGGGCTCACAACTCCGCTCTTCGCCAATGGAATCATTCGCACATTTATGCAAGTTCTGCTGTAGACTTTTACTCCACTGCTTGGGATGTCCTTATTCCGTTCGGAGGTCTGCTGTTTGCTGGCATCATTTTCTTGCAGCAGAAGTTTGGGGGTCTTTGCGTTCTTCCTCCAAAGTTGAGAGATTTTATTGCATATGAGAAGGTTCCGACTGTCGCCGAAACTGAAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
DUF2921

Protein Analysis

946

Amino Acids

106.13

Weight (kDa)

9.23

Isoelectric Point (pI)

48.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2921_N PF25333 65 - 235 5.7e-18 DUF2921 lipocalin-like domain
DUF2921_N PF25333 289 - 425 1.3e-28 DUF2921 lipocalin-like domain
DUF2921_N PF25333 453 - 632 6.2e-32 DUF2921 lipocalin-like domain
DUF2921 PF11145 646 - 927 8e-61 Transmembrane E3 ligase/DUF2921 transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21700
fragaria_vesca FvH4_1g10360 FvH4_1g10760 FvH4_2g30370 FvH4_3g09240 FvH4_3g20050 FvH4_3g20280 FvH4_3g20290 FvH4_3g20291 FvH4_3g20340 FvH4_3g20350 FvH4_3g20351 FvH4_3g20380 FvH4_3g20390 FvH4_5g13190 FvH4_6g20070 FvH4_6g42890
malus_domestica MD03G1227000.v1.1 MD10G1294900.v1.1 MD11G1246300.v1.1 MD11G1246400.v1.1 MD11G1246500.v1.1 MD11G1246600.v1.1
prunus_persica Prupe.4G047300_v2.0.a1 Prupe.4G181700_v2.0.a1 Prupe.4G181900_v2.0.a1 Prupe.4G182000_v2.0.a1 Prupe.4G182200_v2.0.a1
pyrus_communis pycom03g17470 pycom10g24670 pycom11g21640
rosa_chinensis RchiOBHm_Chr5g0008381 RchiOBHm_Chr5g0008391 RchiOBHm_Chr5g0033881 RchiOBHm_Chr5g0034171 RchiOBHm_Chr5g0034181 RchiOBHm_Chr5g0034191 RchiOBHm_Chr5g0034201 RchiOBHm_Chr5g0034211 RchiOBHm_Chr5g0034221 RchiOBHm_Chr5g0034231 RchiOBHm_Chr5g0034271 RchiOBHm_Chr5g0034281
rosa_laevigata RLG00000031566 RLG00000031567 RLG00000033506 RLG00000033529 RLG00000033530 RLG00000033531
rosa_multiflora Rmu_co8445509.1_g000001 Rmu_co8470615.1_g000001 Rmu_sc0008805.1_g000003 Rmu_sc0008805.1_g000004 Rmu_ssc0000128.1_g000029 Rmu_ssc0000128.1_g000031 Rmu_ssc0000128.1_g000032 Rmu_ssc0000388.1_g000062 Rmu_ssc0000388.1_g000063
rosa_roxburghii Rroxscaffold_1G00046010 Rroxscaffold_1G00046020 Rroxscaffold_1G00046030 Rroxscaffold_1G00046040 Rroxscaffold_1G00046060 Rroxscaffold_1G00046070 Rroxscaffold_1G00046080 Rroxscaffold_1G00046090
rosa_rugosa Rorug04G0435500 Rorug05G0142900 Rorug05G0144600 Rorug05G0144600 Rorug05G0144700 Rorug05G0144800
rosa_samantha Rh5AG233800 Rh5AG235900 Rh5AG236000 Rh5AG236100 Rh5AG236200 Rh5AG236300 Rh5AG236500 Rh5BG061200 Rh5BG061300 Rh5BG234300 Rh5BG236600 Rh5BG236800 Rh5CG071200 Rh5DG060000 Rh5DG060100 Rh5DG241300 Rh5DG243700 Rh5DG244000 Rh5DG244100
rosa_wichuraiana Rw3G018950 Rw5G005680 Rw5G021330 Rw5G021340 Rw5G021350 Rw5G021550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 606
AasI GACNNNNNNGTC 1 cut(s) 1586
Acc65I GGTACC 1 cut(s) 1014
AccB1I GGYRCC 2 cut(s) 1014, 2411
AccB7I CCANNNNNTGG 1 cut(s) 2688
AccBSI CCGCTC 1 cut(s) 2624
AccI GTMKAC 2 cut(s) 1334, 2670
AciI CCGC 7 cut(s) 265, 323, 1412, 2175, 2415, 2588, 2622
AclI AACGTT 1 cut(s) 2151
AclWI GGATC 5 cut(s) 550, 1697, 1703, 1874, 1938
AcsI RAATTY 6 cut(s) 297, 616, 989, 1663, 1680, 1802
AcuI CTGAAG 3 cut(s) 360, 1710, 1896
AcyI GRCGYC 1 cut(s) 2412
AfaI GTAC 7 cut(s) 53, 387, 737, 1016, 1138, 1554, 2606
AfiI CCNNNNNNNGG 6 cut(s) 1017, 1712, 1726, 1953, 2688, 2711
AflII CTTAAG 1 cut(s) 596
AflIII ACRYGT 2 cut(s) 2158, 2514
AhdI GACNNNNNGTC 1 cut(s) 819
AhlI ACTAGT 1 cut(s) 632
AjiI CACGTC 1 cut(s) 2050
AjnI CCWGG 3 cut(s) 388, 1010, 1315
AjuI GAANNNNNNNTTGG 2 cut(s) 1399, 1431
Alw21I GWGCWC 5 cut(s) 861, 956, 1384, 2166, 2301
Alw26I GTCTC 6 cut(s) 463, 706, 806, 990, 1492, 2325
AlwI GGATC 5 cut(s) 550, 1697, 1703, 1874, 1938
AlwNI CAGNNNCTG 1 cut(s) 2506
Ama87I CYCGRG 1 cut(s) 1366
AoxI GGCC 3 cut(s) 1022, 1219, 1747
ApaI GGGCCC 1 cut(s) 1751
ApeKI GCWGC 9 cut(s) 92, 908, 1478, 1851, 2254, 2348, 2363, 2450, 2743
ApoI RAATTY 6 cut(s) 297, 616, 989, 1663, 1680, 1802
ArsI GACNNNNNNTTYG 2 cut(s) 1415, 1447
Asp700I GAANNNNTTC 1 cut(s) 2538
Asp718I GGTACC 1 cut(s) 1014
AspLEI GCGC 1 cut(s) 2414
AspS9I GGNCC 5 cut(s) 1023, 1219, 1747, 1748, 2391
AsuHPI GGTGA 1 cut(s) 782
AvaI CYCGRG 1 cut(s) 1366
AvaII GGWCC 1 cut(s) 2391
BaeGI GKGCMC 2 cut(s) 1367, 1751
BaeI ACNNNNGTAYC 2 cut(s) 369, 402
BanI GGYRCC 2 cut(s) 1014, 2411
BanII GRGCYC 5 cut(s) 662, 861, 1751, 2301, 2614
BarI GAAGNNNNNNTAC 2 cut(s) 35, 67
BauI CACGAG 1 cut(s) 332
BbsI GAAGAC 5 cut(s) 15, 407, 850, 1902, 2240
Bbv12I GWGCWC 5 cut(s) 861, 956, 1384, 2166, 2301
BbvI GCAGC 9 cut(s) 79, 895, 1465, 1863, 2266, 2335, 2350, 2437, 2755
BccI CCATC 7 cut(s) 1499, 1518, 1706, 1951, 2175, 2473, 2477
BceAI ACGGC 1 cut(s) 864
BciT130I CCWGG 3 cut(s) 390, 1012, 1317
BclI TGATCA 2 cut(s) 181, 199
BcoDI GTCTC 6 cut(s) 463, 706, 806, 990, 1492, 2325
BcuI ACTAGT 1 cut(s) 632
BfaI CTAG 1 cut(s) 633
BfmI CTRYAG 6 cut(s) 1567, 1779, 1926, 2252, 2349, 2667
BfoI RGCGCY 1 cut(s) 2415
BfrI CTTAAG 1 cut(s) 596
BglII AGATCT 1 cut(s) 1655
BmcAI AGTACT 2 cut(s) 737, 1138
Bme1390I CCNGG 3 cut(s) 390, 1012, 1317
Bme18I GGWCC 1 cut(s) 2391
BmeRI GACNNNNNGTC 1 cut(s) 819
BmeT110I CYCGRG 1 cut(s) 1366
BmgBI CACGTC 1 cut(s) 2050
BmgT120I GGNCC 5 cut(s) 1023, 1219, 1747, 1748, 2391
BmiI GGNNCC 5 cut(s) 649, 1016, 1749, 2413, 2811
BmrFI CCNGG 3 cut(s) 390, 1012, 1317
BmsI GCATC 8 cut(s) 1057, 1067, 1278, 1620, 1706, 1724, 1951, 2739
BpiI GAAGAC 5 cut(s) 15, 407, 850, 1902, 2240
BpmI CTGGAG 2 cut(s) 385, 1299
BpuEI CTTGAG 2 cut(s) 853, 1192
BsaHI GRCGYC 1 cut(s) 2412
BsaJI CCNNGG 3 cut(s) 1010, 1974, 2167
BsaWI WCCGGW 2 cut(s) 901, 1017
BsaXI ACNNNNNCTCC 4 cut(s) 2148, 2178, 2663, 2693
Bsc4I CCNNNNNNNGG 6 cut(s) 1017, 1712, 1726, 1953, 2688, 2711
Bse118I RCCGGY 1 cut(s) 2468
Bse1I ACTGG 4 cut(s) 155, 289, 698, 824
Bse3DI GCAATG 2 cut(s) 56, 874
BseBI CCWGG 3 cut(s) 390, 1012, 1317
BseDI CCNNGG 3 cut(s) 1010, 1974, 2167
BseLI CCNNNNNNNGG 6 cut(s) 1017, 1712, 1726, 1953, 2688, 2711
BseMI GCAATG 2 cut(s) 56, 874
BseMII CTCAG 2 cut(s) 1301, 2513
BseNI ACTGG 4 cut(s) 155, 289, 698, 824
BseSI GKGCMC 2 cut(s) 1367, 1751
BseXI GCAGC 9 cut(s) 79, 895, 1465, 1863, 2266, 2335, 2350, 2437, 2755
Bsh1285I CGRYCG 1 cut(s) 1197
BshFI GGCC 3 cut(s) 1024, 1221, 1749
BshNI GGYRCC 2 cut(s) 1014, 2411
BsiEI CGRYCG 1 cut(s) 1197
BsiHKAI GWGCWC 5 cut(s) 861, 956, 1384, 2166, 2301
BsiHKCI CYCGRG 1 cut(s) 1366
BsiSI CCGG 3 cut(s) 902, 1018, 2469
BslFI GGGAC 1 cut(s) 576
BslI CCNNNNNNNGG 6 cut(s) 1017, 1712, 1726, 1953, 2688, 2711
BsmAI GTCTC 6 cut(s) 463, 706, 806, 990, 1492, 2325
BsmFI GGGAC 1 cut(s) 576
BsmI GAATGC 2 cut(s) 1117, 1837
BsnI GGCC 3 cut(s) 1024, 1221, 1749
BsoBI CYCGRG 1 cut(s) 1366
Bsp120I GGGCCC 1 cut(s) 1747
Bsp1286I GDGCHC 9 cut(s) 662, 861, 956, 1367, 1384, 1751, 2166, 2301, 2614
Bsp1407I TGTACA 1 cut(s) 2604
BspACI CCGC 7 cut(s) 265, 323, 1412, 2175, 2415, 2588, 2622
BspANI GGCC 3 cut(s) 1024, 1221, 1749
BspCNI CTCAG 2 cut(s) 1300, 2512
BspHI TCATGA 3 cut(s) 6, 399, 612
BspLI GGNNCC 5 cut(s) 649, 1016, 1749, 2413, 2811
BspMAI CTGCAG 2 cut(s) 2256, 2353
BspPI GGATC 5 cut(s) 550, 1697, 1703, 1874, 1938
BspQI GCTCTTC 1 cut(s) 2631
BspT107I GGYRCC 2 cut(s) 1014, 2411
BspTI CTTAAG 1 cut(s) 596
BsrBI CCGCTC 1 cut(s) 2624
BsrDI GCAATG 2 cut(s) 56, 874
BsrFI RCCGGY 1 cut(s) 2468
BsrGI TGTACA 1 cut(s) 2604
BsrI ACTGG 4 cut(s) 155, 289, 698, 824
BssAI RCCGGY 1 cut(s) 2468
BssECI CCNNGG 3 cut(s) 1010, 1974, 2167
BssNAI GTATAC 1 cut(s) 1335
BssNI GRCGYC 1 cut(s) 2412
BssSI CACGAG 1 cut(s) 332
BssT1I CCWWGG 2 cut(s) 1974, 2167
Bst1107I GTATAC 1 cut(s) 1335
Bst2BI CACGAG 1 cut(s) 332
Bst2UI CCWGG 3 cut(s) 390, 1012, 1317
Bst4CI ACNGT 9 cut(s) 206, 687, 767, 817, 1001, 1216, 1757, 1927, 2818
Bst6I CTCTTC 3 cut(s) 1890, 2030, 2631
BstACI GRCGYC 1 cut(s) 2412
BstAFI CTTAAG 1 cut(s) 596
BstAPI GCANNNNNTGC 1 cut(s) 2663
BstAUI TGTACA 1 cut(s) 2604
BstC8I GCNNGC 4 cut(s) 1439, 1537, 2592, 2728
BstDEI CTNAG 6 cut(s) 497, 652, 672, 1287, 2087, 2499
BstH2I RGCGCY 1 cut(s) 2415
BstHHI GCGC 1 cut(s) 2414
BstMAI GTCTC 6 cut(s) 463, 706, 806, 990, 1492, 2325
BstMCI CGRYCG 1 cut(s) 1197
BstMWI GCNNNNNNNGC 6 cut(s) 1218, 1545, 1860, 2354, 2363, 2663
BstNI CCWGG 3 cut(s) 390, 1012, 1317
BstNSI RCATGY 6 cut(s) 1429, 1441, 2004, 2162, 2518, 2594
BstSCI CCNGG 3 cut(s) 388, 1010, 1315
BstSFI CTRYAG 6 cut(s) 1567, 1779, 1926, 2252, 2349, 2667
BstSLI GKGCMC 2 cut(s) 1367, 1751
BstV1I GCAGC 9 cut(s) 79, 895, 1465, 1863, 2266, 2335, 2350, 2437, 2755
BstV2I GAAGAC 5 cut(s) 15, 407, 850, 1902, 2240
BstX2I RGATCY 2 cut(s) 1655, 1695
BstXI CCANNNNNNTGG 2 cut(s) 1517, 2408
BstYI RGATCY 2 cut(s) 1655, 1695
BstZ17I GTATAC 1 cut(s) 1335
BsuRI GGCC 3 cut(s) 1024, 1221, 1749
BtgZI GCGATG 3 cut(s) 102, 147, 1370
BtrI CACGTC 1 cut(s) 2050
BtsI GCAGTG 3 cut(s) 439, 1480, 2682
BtsIMutI CAGTG 7 cut(s) 202, 282, 439, 831, 1212, 1480, 2682
Cac8I GCNNGC 4 cut(s) 1439, 1537, 2592, 2728
CaiI CAGNNNCTG 1 cut(s) 2506
CciI TCATGA 3 cut(s) 6, 399, 612
CfoI GCGC 1 cut(s) 2414
Cfr10I RCCGGY 1 cut(s) 2468
Cfr13I GGNCC 5 cut(s) 1023, 1219, 1747, 1748, 2391
Csp6I GTAC 7 cut(s) 52, 386, 736, 1015, 1137, 1553, 2605
CviQI GTAC 7 cut(s) 52, 386, 736, 1015, 1137, 1553, 2605
DdeI CTNAG 6 cut(s) 497, 652, 672, 1287, 2087, 2499
DinI GGCGCC 1 cut(s) 2413
DraI TTTAAA 2 cut(s) 493, 1527
DrdI GACNNNNNNGTC 1 cut(s) 1586
DriI GACNNNNNGTC 1 cut(s) 819
DseDI GACNNNNNNGTC 1 cut(s) 1586
Eam1104I CTCTTC 3 cut(s) 1890, 2030, 2631
Eam1105I GACNNNNNGTC 1 cut(s) 819
EarI CTCTTC 3 cut(s) 1890, 2030, 2631
EciI GGCGGA 1 cut(s) 312
Ecl136II GAGCTC 2 cut(s) 859, 2299
Eco130I CCWWGG 2 cut(s) 1974, 2167
Eco24I GRGCYC 5 cut(s) 662, 861, 1751, 2301, 2614
Eco32I GATATC 1 cut(s) 1986
Eco47I GGWCC 1 cut(s) 2391
Eco53kI GAGCTC 2 cut(s) 859, 2299
Eco57I CTGAAG 3 cut(s) 360, 1710, 1896
Eco88I CYCGRG 1 cut(s) 1366
EcoICRI GAGCTC 2 cut(s) 859, 2299
EcoO109I RGGNCCY 1 cut(s) 1023
EcoRI GAATTC 2 cut(s) 297, 616
EcoRII CCWGG 3 cut(s) 388, 1010, 1315
EcoRV GATATC 1 cut(s) 1986
EcoT14I CCWWGG 2 cut(s) 1974, 2167
EcoT22I ATGCAT 1 cut(s) 2596
EcoT38I GRGCYC 5 cut(s) 662, 861, 1751, 2301, 2614
EgeI GGCGCC 1 cut(s) 2413
EheI GGCGCC 1 cut(s) 2413
ErhI CCWWGG 2 cut(s) 1974, 2167
FalI AAGNNNNNCTT 4 cut(s) 399, 431, 2309, 2341
FaqI GGGAC 1 cut(s) 576
FauNDI CATATG 3 cut(s) 241, 2596, 2800
FbaI TGATCA 2 cut(s) 181, 199
FblI GTMKAC 2 cut(s) 1334, 2670
FriOI GRGCYC 5 cut(s) 662, 861, 1751, 2301, 2614
FspBI CTAG 1 cut(s) 633
GlaI GCGC 1 cut(s) 2413
GsuI CTGGAG 2 cut(s) 385, 1299
HaeII RGCGCY 1 cut(s) 2415
HaeIII GGCC 3 cut(s) 1024, 1221, 1749
HapII CCGG 3 cut(s) 902, 1018, 2469
HhaI GCGC 1 cut(s) 2414
Hin1I GRCGYC 1 cut(s) 2412
Hin6I GCGC 1 cut(s) 2412
HinP1I GCGC 1 cut(s) 2412
HincII GTYRAC 3 cut(s) 1591, 1760, 2194
HindII GTYRAC 3 cut(s) 1591, 1760, 2194
HindIII AAGCTT 3 cut(s) 65, 487, 599
HpaI GTTAAC 1 cut(s) 2194
HpaII CCGG 3 cut(s) 902, 1018, 2469
HphI GGTGA 1 cut(s) 782
Hpy166II GTNNAC 5 cut(s) 1335, 1591, 1760, 2194, 2671
Hpy8I GTNNAC 5 cut(s) 1335, 1591, 1760, 2194, 2671
Hpy99I CGWCG 1 cut(s) 2051
HpyCH4III ACNGT 9 cut(s) 206, 687, 767, 817, 1001, 1216, 1757, 1927, 2818
HpyCH4IV ACGT 2 cut(s) 2049, 2151
HpyF10VI GCNNNNNNNGC 6 cut(s) 1218, 1545, 1860, 2354, 2363, 2663
HpyF3I CTNAG 6 cut(s) 497, 652, 672, 1287, 2087, 2499
HpySE526I ACGT 2 cut(s) 2049, 2151
Hsp92I GRCGYC 1 cut(s) 2412
HspAI GCGC 1 cut(s) 2412
KasI GGCGCC 1 cut(s) 2411
KpnI GGTACC 1 cut(s) 1018
Ksp22I TGATCA 2 cut(s) 181, 199
KspAI GTTAAC 1 cut(s) 2194
LguI GCTCTTC 1 cut(s) 2631
LmnI GCTCC 4 cut(s) 1230, 1486, 2169, 2296
Lsp1109I GCAGC 9 cut(s) 79, 895, 1465, 1863, 2266, 2335, 2350, 2437, 2755
LweI GCATC 8 cut(s) 1057, 1067, 1278, 1620, 1706, 1724, 1951, 2739
MaeI CTAG 1 cut(s) 633
MaeII ACGT 2 cut(s) 2049, 2151
MaeIII GTNAC 5 cut(s) 275, 628, 1256, 1351, 2215
MbiI CCGCTC 1 cut(s) 2624
MfeI CAATTG 1 cut(s) 213
MflI RGATCY 2 cut(s) 1655, 1695
MhlI GDGCHC 9 cut(s) 662, 861, 956, 1367, 1384, 1751, 2166, 2301, 2614
Mly113I GGCGCC 1 cut(s) 2412
MlyI GAGTC 1 cut(s) 1883
MmeI TCCRAC 3 cut(s) 520, 1841, 2837
Mph1103I ATGCAT 1 cut(s) 2596
MroXI GAANNNNTTC 1 cut(s) 2538
MseI TTAA 4 cut(s) 492, 597, 1526, 2193
MslI CAYNNNNRTG 7 cut(s) 1146, 1515, 1999, 2072, 2135, 2595, 2652
MspCI CTTAAG 1 cut(s) 596
MspI CCGG 3 cut(s) 902, 1018, 2469
MspR9I CCNGG 3 cut(s) 390, 1012, 1317
MunI CAATTG 1 cut(s) 213
Mva1269I GAATGC 2 cut(s) 1117, 1837
MvaI CCWGG 3 cut(s) 390, 1012, 1317
MwoI GCNNNNNNNGC 6 cut(s) 1218, 1545, 1860, 2354, 2363, 2663
NarI GGCGCC 1 cut(s) 2412
NdeI CATATG 3 cut(s) 241, 2596, 2800
NlaIV GGNNCC 5 cut(s) 649, 1016, 1749, 2413, 2811
NsiI ATGCAT 1 cut(s) 2596
NspI RCATGY 6 cut(s) 1429, 1441, 2004, 2162, 2518, 2594
PaeI GCATGC 2 cut(s) 1441, 2594
PagI TCATGA 3 cut(s) 6, 399, 612
PciI ACATGT 2 cut(s) 2158, 2514
PciSI GCTCTTC 1 cut(s) 2631
PctI GAATGC 2 cut(s) 1117, 1837
PdmI GAANNNNTTC 1 cut(s) 2538
PflFI GACNNNGTC 1 cut(s) 567
PflMI CCANNNNNTGG 1 cut(s) 2688
Ple19I CGATCG 1 cut(s) 1197
PleI GAGTC 1 cut(s) 1882
PluTI GGCGCC 1 cut(s) 2415
PpsI GAGTC 1 cut(s) 1882
PscI ACATGT 2 cut(s) 2158, 2514
PsiI TTATAA 1 cut(s) 606
Psp124BI GAGCTC 2 cut(s) 861, 2301
Psp1406I AACGTT 1 cut(s) 2151
Psp6I CCWGG 3 cut(s) 388, 1010, 1315
PspGI CCWGG 3 cut(s) 388, 1010, 1315
PspN4I GGNNCC 5 cut(s) 649, 1016, 1749, 2413, 2811
PspOMI GGGCCC 1 cut(s) 1747
PspPI GGNCC 5 cut(s) 1023, 1219, 1747, 1748, 2391
PsrI GAACNNNNNNTAC 2 cut(s) 1536, 1568
PstI CTGCAG 2 cut(s) 2256, 2353
PstNI CAGNNNCTG 1 cut(s) 2506
PsuI RGATCY 2 cut(s) 1655, 1695
PsyI GACNNNGTC 1 cut(s) 567
PvuI CGATCG 1 cut(s) 1197
RsaI GTAC 7 cut(s) 53, 387, 737, 1016, 1138, 1554, 2606
RsaNI GTAC 7 cut(s) 52, 386, 736, 1015, 1137, 1553, 2605
RseI CAYNNNNRTG 7 cut(s) 1146, 1515, 1999, 2072, 2135, 2595, 2652
SacI GAGCTC 2 cut(s) 861, 2301
SapI GCTCTTC 1 cut(s) 2631
SaqAI TTAA 4 cut(s) 492, 597, 1526, 2193
Sau96I GGNCC 5 cut(s) 1023, 1219, 1747, 1748, 2391
ScaI AGTACT 2 cut(s) 737, 1138
SchI GAGTC 1 cut(s) 1883
ScrFI CCNGG 3 cut(s) 390, 1012, 1317
SduI GDGCHC 9 cut(s) 662, 861, 956, 1367, 1384, 1751, 2166, 2301, 2614
SfaNI GCATC 8 cut(s) 1057, 1067, 1278, 1620, 1706, 1724, 1951, 2739
SfcI CTRYAG 6 cut(s) 1567, 1779, 1926, 2252, 2349, 2667
SfoI GGCGCC 1 cut(s) 2413
SinI GGWCC 1 cut(s) 2391
SmiI ATTTAAAT 1 cut(s) 1527
SmiMI CAYNNNNRTG 7 cut(s) 1146, 1515, 1999, 2072, 2135, 2595, 2652
SmlI CTYRAG 3 cut(s) 596, 868, 1171
SmoI CTYRAG 3 cut(s) 596, 868, 1171
SpeI ACTAGT 1 cut(s) 632
SphI GCATGC 2 cut(s) 1441, 2594
SsiI CCGC 7 cut(s) 265, 323, 1412, 2175, 2415, 2588, 2622
SspDI GGCGCC 1 cut(s) 2411
SspI AATATT 1 cut(s) 787
SspMI CTAG 1 cut(s) 633
SstI GAGCTC 2 cut(s) 861, 2301
StyD4I CCNGG 3 cut(s) 388, 1010, 1315
StyI CCWWGG 2 cut(s) 1974, 2167
SwaI ATTTAAAT 1 cut(s) 1527
TaaI ACNGT 9 cut(s) 206, 687, 767, 817, 1001, 1216, 1757, 1927, 2818
TaiI ACGT 2 cut(s) 2052, 2154
TaqI TCGA 5 cut(s) 472, 704, 1102, 2528, 2578
TatI WGTACW 3 cut(s) 735, 1136, 2604
TauI GCSGC 2 cut(s) 2417, 2590
Tru1I TTAA 4 cut(s) 492, 597, 1526, 2193
Tru9I TTAA 4 cut(s) 492, 597, 1526, 2193
TscAI CASTG 7 cut(s) 209, 289, 439, 831, 1219, 1480, 2689
TseI GCWGC 9 cut(s) 92, 908, 1478, 1851, 2254, 2348, 2363, 2450, 2743
TspDTI ATGAA 9 cut(s) 23, 105, 150, 388, 629, 680, 1877, 2062, 2298
TspGWI ACGGA 3 cut(s) 1882, 2234, 2694
TspRI CASTG 7 cut(s) 209, 289, 439, 831, 1219, 1480, 2689
Tth111I GACNNNGTC 1 cut(s) 567
Van91I CCANNNNNTGG 1 cut(s) 2688
Vha464I CTTAAG 1 cut(s) 596
VpaK11BI GGWCC 1 cut(s) 2391
XapI RAATTY 6 cut(s) 297, 616, 989, 1663, 1680, 1802
XceI RCATGY 6 cut(s) 1429, 1441, 2004, 2162, 2518, 2594
XcmI CCANNNNNNNNNTGG 2 cut(s) 1042, 1954
XmiI GTMKAC 2 cut(s) 1334, 2670
XmnI GAANNNNTTC 1 cut(s) 2538
XspI CTAG 1 cut(s) 633
ZrmI AGTACT 2 cut(s) 737, 1138
Zsp2I ATGCAT 1 cut(s) 2596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.