Rh5BG061200

Protein of unknown function (DUF2921)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000420
Physical Location & Seq
Reverse (-)
5047489 .. 5050338
2850 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG061200.1

Sequence Viewer

Length: 2850 bp
ATGGAGACCAATTTAAAATCTAGGGTTGGGGCTCTTCCAATTTCCCCAACCACTCCTCTACAAACCCATAAATCCCTACTCACTCCCCTCCTCTTCTTCTTCTTCATCAATTTCACCCCCACAGCTTCAACCCTGAACCAAATCCCCAATCTTGATCAACTATACTCCAGACACTGCAACGACGTCGTTCCCAAATCGGAGACGCACCCCAAGTGGTTCCTCACCACCCTCTCCATCCAAGACCTCGGATTCCACAGCAGTTACTTCACCGGCGGCGATCGCCTTTTCAATCAGAGCTCCGGCGGCCGCAAAGCTCTCATCTTCCGGCCCAACTACGTAGCTCGAACCCTAGCCGATGGCATTTTCCATGTCAAAGGAAGTATGGATCTTCAATCTCTTCTGGGCTATTCGACTCGCCGGAATCTGAAGTTGGTTAAGCCCAGAATTCCGGTCCGGCGGGGCGTAGTGAGGTTTAGGCTTGATGGGTATTATTCAGAATCGTCACAGAAGCTGTGTATGATTGGGAAAGTTATGTCCATGTATAATGCAGGTAAGTTTGAGGCTAGTGTTGTTCTTAAGCTTAATTATCCGAAATGGTCTAGTGTTTATGATAGTTTGATTACTGGGAGTTTAGAGAGCGTTGTTATTGCTGAGGGCGATGATGGTAAGAATTATTTTGATCCCGTTTTGATGTTGGGTTTGTCTGTGAAACGTAGTTATGAGTATACATTTGTTGGGAAAGATAGGCTGAGTAGTTTTCCGAGTAGTGTTGATAGAGGAGAGAGTATAGCTTTGGAGAATTTGAATGATTCGAGACGTGATTTGTGTAGGTTGCTTGGCAACCGTCTTGAGAGATATGAATTGGTGTATGGGAGTGAGTGTGGGGGTGTGAATTGCAATCCTGTTGGTGGGGATGTTGGATATGTACCTGGCTCGTTGGTTTTTTATGGGACTCGGTGTACAGAAGGGAGGAAAGTGCAGATGTTGTTGGGGTTTTCCAACTCCAGTGTAGATGAATACATAAGATTTCCATTTGTGCCTAGCACGGCGTTGATTGCTGAAGGGGAATGGGATGAGAAGGGGAATCGTCTATGCGCGGTTGCGTGTAGGATTTTGAATTTTACAGAATCTTTGACTAGTGCTCGTGTTGGAGACTGCTCAACTAAACTCAGCTTGAGATTTCCAGTAAAGTTGTCTTTACGAAATAGGTCTGCTGTTGTGGGGCAAATTTGGAGCGACAAAAAAGTGAGTGATCCTGGTTACTTCAGTAAAATTGGGTTCCAAGGTTTGTCGGGATGGTTAGTGAAACTCTTAGGATACAAATATGAATATTCTGTGAACGACAACCTGAGAAAAACATGTGCGGAAAAGAGGGCTGGGAGAGGCAAAGGAAAGCGATATCCTGAGGAGTATTCATCAGATCTGAAATTTGACATGAGAGTGAGCAACAGCAAAGGACAAGTTGGATGGGGTTATTCATCGCCATTATTTGTTGATGATGACCGGATTTATGGACGCCGGTTTTGGGATAAACCACAGGAAACAGAAGTACCAAGTCGTCGGAAGAAAAGCCATAGCAGTCCGATGAACATTACCTATAAGTTGAGCTTCACACGTGGTGTTCAATTTTTTCATGATGTCTTCCCATCTAAAGCAGAACTAGCTGCTGAAGGAATATATGATAGAGATTATGGAAACCTATGCATGATAGGATGTAGGCATGTACCATCCAAGGAACAAAATTCGATTAAAAAGGATATGTTAGACTGTTCTGTTAAAATTGATGTACACTTTCCTCCATTGGATACCAAGGATGGCCAGAATGTCAAGGGAACTATTGAAAGCACGAGGAATAAGTCAGACCCTCTTTATTTTGAACGTATTGACATCTCTTCGACTTCAATTTACCATAACCAAGCTGCTGCATCCATTACGAGAATCGATTTTGAGATTGTTATGGTTTTGATTTCCAATACACTTGCATGTGCTTTTGTCGGATGGCAGCTCTATTTTGTGAAGAAAAACCCAGATGTGCTCCCATTCACTTCAATTGTCATGCTCATTGTTCTTACTCTGGGATACGTGATTCCTCTTCTGTTCAACTTTGAAGCAATGCTTGCACCAAACCAAAGCAAGCAGAGTACTTTTCTTGGGATTGGTGGATGGCTCCAGGTTAATGAAGTAATGGTCAGGGTGATAATGTTGGTAACTTTCATTTTGCAGCTGCTTCTTCTCCAACTGACCTGGTCATCGAGACAAGGTGATAATAGCCAGAAGAGCTTGATGGATTCTGAGAGGAAGGTTCTTTATGCCACCTTACTAGTGTACATAGCTGGTGCACTGATTACTTGCTCTGTCCACCAGTGGATGAGTACTCACCAGAACTCATACAGGCCATTTCATCGCAAGCTTCGGCGAGTGGCTAACCTATGGCATCTTGTTCACCCGCAACGCTCTCTCTGGGACGACCTCAAGTCTTATGCTGGATTCATCCTGGACGGTTTTCTACTCCCGCAAATATTGTTCAACTTATTCGTCAATTCAGGTGAAAAGGCTCTGTCCTGTGCCTTTTACTTTGGAACCACCATGATTCGTCTGCTGCCTCATGCTTATGATCTGTACAGAGCTCGCGCTTACACATGGTTACTTGCTTTCTCATATATTTATGCAAACCCCAAAACCGATTTTTTCTCCACTGCCTGGAACATAATCATACCCTGTTGTTGTCTGCTGCTTGCTGCCATTATTTTCTTGCAGCAAAGGTTTGGCGGCCGTTGCATCCTTCCCAAAATATTTGGGCATTCCACTGTATATGAAAAGGTCCCTGTCATCAGCAATGAGGAATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
DUF2921

Protein Analysis

949

Amino Acids

107.56

Weight (kDa)

9.26

Isoelectric Point (pI)

40.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2921_N PF25333 55 - 229 4.1e-18 DUF2921 lipocalin-like domain
DUF2921_N PF25333 253 - 425 4.4e-30 DUF2921 lipocalin-like domain
DUF2921_N PF25333 456 - 629 2e-33 DUF2921 lipocalin-like domain
DUF2921 PF11145 640 - 932 7.7e-59 Transmembrane E3 ligase/DUF2921 transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21700
fragaria_vesca FvH4_1g10360 FvH4_1g10760 FvH4_2g30370 FvH4_3g09240 FvH4_3g20050 FvH4_3g20280 FvH4_3g20290 FvH4_3g20291 FvH4_3g20340 FvH4_3g20350 FvH4_3g20351 FvH4_3g20380 FvH4_3g20390 FvH4_5g13190 FvH4_6g20070 FvH4_6g42890
malus_domestica MD03G1227000.v1.1 MD10G1294900.v1.1 MD11G1246300.v1.1 MD11G1246400.v1.1 MD11G1246500.v1.1 MD11G1246600.v1.1
prunus_persica Prupe.4G047300_v2.0.a1 Prupe.4G181700_v2.0.a1 Prupe.4G181900_v2.0.a1 Prupe.4G182000_v2.0.a1 Prupe.4G182200_v2.0.a1
pyrus_communis pycom03g17470 pycom10g24670 pycom11g21640
rosa_chinensis RchiOBHm_Chr5g0008381 RchiOBHm_Chr5g0008391 RchiOBHm_Chr5g0033881 RchiOBHm_Chr5g0034171 RchiOBHm_Chr5g0034181 RchiOBHm_Chr5g0034191 RchiOBHm_Chr5g0034201 RchiOBHm_Chr5g0034211 RchiOBHm_Chr5g0034221 RchiOBHm_Chr5g0034231 RchiOBHm_Chr5g0034271 RchiOBHm_Chr5g0034281
rosa_laevigata RLG00000031566 RLG00000031567 RLG00000033506 RLG00000033529 RLG00000033530 RLG00000033531
rosa_multiflora Rmu_co8445509.1_g000001 Rmu_co8470615.1_g000001 Rmu_sc0008805.1_g000003 Rmu_sc0008805.1_g000004 Rmu_ssc0000128.1_g000029 Rmu_ssc0000128.1_g000031 Rmu_ssc0000128.1_g000032 Rmu_ssc0000388.1_g000062 Rmu_ssc0000388.1_g000063
rosa_roxburghii Rroxscaffold_1G00046010 Rroxscaffold_1G00046020 Rroxscaffold_1G00046030 Rroxscaffold_1G00046040 Rroxscaffold_1G00046060 Rroxscaffold_1G00046070 Rroxscaffold_1G00046080 Rroxscaffold_1G00046090
rosa_rugosa Rorug04G0435500 Rorug05G0142900 Rorug05G0144600 Rorug05G0144600 Rorug05G0144700 Rorug05G0144800
rosa_samantha Rh5AG233800 Rh5AG235900 Rh5AG236000 Rh5AG236100 Rh5AG236200 Rh5AG236300 Rh5AG236500 Rh5BG061200 Rh5BG061300 Rh5BG234300 Rh5BG236600 Rh5BG236800 Rh5CG071200 Rh5DG060000 Rh5DG060100 Rh5DG241300 Rh5DG243700 Rh5DG244000 Rh5DG244100
rosa_wichuraiana Rw3G018950 Rw5G005680 Rw5G021330 Rw5G021340 Rw5G021350 Rw5G021550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 186
Acc36I ACCTGC 1 cut(s) 539
AccB7I CCANNNNNTGG 1 cut(s) 2700
AccI GTMKAC 1 cut(s) 725
AccII CGCG 2 cut(s) 1097, 2631
AciI CCGC 9 cut(s) 273, 303, 307, 457, 1097, 1364, 2447, 2513, 2769
AclWI GGATC 3 cut(s) 393, 674, 1247
AcoI YGGCCR 3 cut(s) 304, 1816, 2770
AcsI RAATTY 6 cut(s) 444, 799, 1117, 1227, 1427, 1741
AcuI CTGAAG 4 cut(s) 446, 1080, 1249, 1689
AcvI CACGTG 1 cut(s) 1616
AcyI GRCGYC 2 cut(s) 183, 1516
AdeI CACNNNGTG 1 cut(s) 1619
AfaI GTAC 9 cut(s) 927, 961, 1551, 1725, 1788, 2143, 2327, 2374, 2621
AfiI CCNNNNNNNGG 3 cut(s) 908, 1525, 2700
AflII CTTAAG 1 cut(s) 575
AflIII ACRYGT 2 cut(s) 1358, 1613
AhdI GACNNNNNGTC 1 cut(s) 2473
AhlI ACTAGT 2 cut(s) 1136, 2320
AjiI CACGTC 1 cut(s) 818
AjnI CCWGG 6 cut(s) 928, 1255, 2169, 2243, 2493, 2699
AjuI GAANNNNNNNTTGG 2 cut(s) 413, 445
Alw21I GWGCWC 5 cut(s) 299, 1144, 2037, 2341, 2629
Alw26I GTCTC 4 cut(s) 194, 808, 1146, 2248
Alw44I GTGCAC 1 cut(s) 2337
AlwI GGATC 3 cut(s) 393, 674, 1247
AlwNI CAGNNNCTG 2 cut(s) 174, 511
AoxI GGCC 5 cut(s) 304, 326, 1816, 2393, 2770
ApaLI GTGCAC 1 cut(s) 2337
ApoI RAATTY 6 cut(s) 444, 799, 1117, 1227, 1427, 1741
ArsI GACNNNNNNTTYG 2 cut(s) 1506, 1538
AsiSI GCGATCGC 1 cut(s) 280
AspLEI GCGC 2 cut(s) 1097, 2633
AspS9I GGNCC 3 cut(s) 327, 451, 2821
AsuHPI GGTGA 8 cut(s) 106, 214, 259, 2206, 2273, 2369, 2435, 2558
AvaII GGWCC 2 cut(s) 451, 2821
AxyI CCTNAGG 1 cut(s) 1404
BaeGI GKGCMC 1 cut(s) 2341
BaeI ACNNNNGTAYC 2 cut(s) 1533, 1566
BalI TGGCCA 1 cut(s) 1818
BanII GRGCYC 3 cut(s) 34, 299, 2629
BauI CACGAG 2 cut(s) 1143, 1846
BbrPI CACGTG 1 cut(s) 1616
BbsI GAAGAC 1 cut(s) 1633
Bbv12I GWGCWC 5 cut(s) 299, 1144, 2037, 2341, 2629
BbvCI CCTCAGC 1 cut(s) 651
BceAI ACGGC 2 cut(s) 1062, 2757
BcgI CGANNNNNNTGC 2 cut(s) 166, 200
BciT130I CCWGG 6 cut(s) 930, 1257, 2171, 2245, 2495, 2701
BciVI GTATCC 3 cut(s) 1310, 1798, 2072
BclI TGATCA 1 cut(s) 154
BcoDI GTCTC 4 cut(s) 194, 808, 1146, 2248
BcuI ACTAGT 2 cut(s) 1136, 2320
BfaI CTAG 8 cut(s) 21, 350, 564, 600, 1041, 1137, 1661, 2321
BfrI CTTAAG 1 cut(s) 575
BfuAI ACCTGC 1 cut(s) 539
BfuI GTATCC 3 cut(s) 1310, 1798, 2072
BglII AGATCT 1 cut(s) 1420
BmcAI AGTACT 2 cut(s) 2143, 2374
Bme1390I CCNGG 6 cut(s) 930, 1257, 2171, 2245, 2495, 2701
Bme18I GGWCC 2 cut(s) 451, 2821
BmeRI GACNNNNNGTC 1 cut(s) 2473
BmgBI CACGTC 1 cut(s) 818
BmgT120I GGNCC 3 cut(s) 327, 451, 2821
BmiI GGNNCC 5 cut(s) 218, 1280, 2168, 2581, 2823
BmrFI CCNGG 6 cut(s) 930, 1257, 2171, 2245, 2495, 2701
BmrI ACTGGG 1 cut(s) 633
BmsI GCATC 3 cut(s) 1934, 2443, 2787
BmuI ACTGGG 1 cut(s) 633
BpiI GAAGAC 1 cut(s) 1633
BpmI CTGGAG 3 cut(s) 151, 988, 2153
Bpu10I CCTNAGC 1 cut(s) 651
BpuEI CTTGAG 3 cut(s) 869, 1195, 2456
Bsa29I ATCGAT 1 cut(s) 1941
BsaAI YACGTR 3 cut(s) 337, 1616, 2083
BsaHI GRCGYC 2 cut(s) 183, 1516
BsaJI CCNNGG 4 cut(s) 244, 1282, 1731, 1809
BsaWI WCCGGW 2 cut(s) 448, 1503
BsaXI ACNNNNNCTCC 2 cut(s) 865, 895
Bsc4I CCNNNNNNNGG 3 cut(s) 908, 1525, 2700
Bse118I RCCGGY 2 cut(s) 269, 1518
Bse1I ACTGG 4 cut(s) 628, 1005, 1184, 2362
Bse21I CCTNAGG 1 cut(s) 1404
Bse3DI GCAATG 2 cut(s) 2118, 2842
BseBI CCWGG 6 cut(s) 930, 1257, 2171, 2245, 2495, 2701
BseCI ATCGAT 1 cut(s) 1941
BseDI CCNNGG 4 cut(s) 244, 1282, 1731, 1809
BseLI CCNNNNNNNGG 3 cut(s) 908, 1525, 2700
BseMI GCAATG 2 cut(s) 2118, 2842
BseMII CTCAG 6 cut(s) 642, 740, 1183, 1340, 1395, 2283
BseNI ACTGG 4 cut(s) 628, 1005, 1184, 2362
BseRI GAGGAG 4 cut(s) 45, 80, 792, 1421
BseSI GKGCMC 1 cut(s) 2341
BseX3I CGGCCG 2 cut(s) 304, 2770
BseYI CCCAGC 1 cut(s) 1376
BsgI GTGCAG 1 cut(s) 998
Bsh1236I CGCG 2 cut(s) 1097, 2631
Bsh1285I CGRYCG 3 cut(s) 280, 307, 2773
BshFI GGCC 5 cut(s) 306, 328, 1818, 2395, 2772
BshVI ATCGAT 1 cut(s) 1941
BsiEI CGRYCG 3 cut(s) 280, 307, 2773
BsiHKAI GWGCWC 5 cut(s) 299, 1144, 2037, 2341, 2629
BsiSI CCGG 8 cut(s) 270, 300, 325, 418, 449, 454, 1504, 1519
BslFI GGGAC 3 cut(s) 964, 2477, 2807
BslI CCNNNNNNNGG 3 cut(s) 908, 1525, 2700
BsmAI GTCTC 4 cut(s) 194, 808, 1146, 2248
BsmBI CGTCTC 2 cut(s) 194, 808
BsmFI GGGAC 3 cut(s) 964, 2477, 2807
BsmI GAATGC 1 cut(s) 2800
BsnI GGCC 5 cut(s) 306, 328, 1818, 2395, 2772
Bsp1286I GDGCHC 6 cut(s) 34, 299, 1144, 2037, 2341, 2629
Bsp1407I TGTACA 4 cut(s) 959, 1786, 2325, 2619
Bsp143I GATC 7 cut(s) 154, 277, 385, 679, 1252, 1420, 2614
BspACI CCGC 9 cut(s) 273, 303, 307, 457, 1097, 1364, 2447, 2513, 2769
BspANI GGCC 5 cut(s) 306, 328, 1818, 2395, 2772
BspCNI CTCAG 6 cut(s) 643, 741, 1182, 1341, 1396, 2284
BspDI ATCGAT 1 cut(s) 1941
BspFNI CGCG 2 cut(s) 1097, 2631
BspHI TCATGA 1 cut(s) 1633
BspLI GGNNCC 5 cut(s) 218, 1280, 2168, 2581, 2823
BspMI ACCTGC 1 cut(s) 539
BspPI GGATC 3 cut(s) 393, 674, 1247
BspQI GCTCTTC 2 cut(s) 39, 2270
BspTI CTTAAG 1 cut(s) 575
BsrDI GCAATG 2 cut(s) 2118, 2842
BsrFI RCCGGY 2 cut(s) 269, 1518
BsrGI TGTACA 4 cut(s) 959, 1786, 2325, 2619
BsrI ACTGG 4 cut(s) 628, 1005, 1184, 2362
BssAI RCCGGY 2 cut(s) 269, 1518
BssECI CCNNGG 4 cut(s) 244, 1282, 1731, 1809
BssMI GATC 7 cut(s) 154, 277, 385, 679, 1252, 1420, 2614
BssNAI GTATAC 1 cut(s) 726
BssNI GRCGYC 2 cut(s) 183, 1516
BssSI CACGAG 2 cut(s) 1143, 1846
BssT1I CCWWGG 3 cut(s) 1282, 1731, 1809
Bst1107I GTATAC 1 cut(s) 726
Bst2BI CACGAG 2 cut(s) 1143, 1846
Bst2UI CCWGG 6 cut(s) 930, 1257, 2171, 2245, 2495, 2701
Bst4CI ACNGT 4 cut(s) 845, 1769, 2501, 2809
Bst6I CTCTTC 6 cut(s) 39, 98, 402, 1897, 2097, 2270
BstACI GRCGYC 2 cut(s) 183, 1516
BstAFI CTTAAG 1 cut(s) 575
BstAPI GCANNNNNTGC 1 cut(s) 2117
BstAUI TGTACA 4 cut(s) 959, 1786, 2325, 2619
BstBAI YACGTR 3 cut(s) 337, 1616, 2083
BstC8I GCNNGC 5 cut(s) 2118, 2135, 2408, 2629, 2736
BstDEI CTNAG 7 cut(s) 651, 749, 1169, 1312, 1349, 1404, 2292
BstFNI CGCG 2 cut(s) 1097, 2631
BstHHI GCGC 2 cut(s) 1097, 2633
BstKTI GATC 7 cut(s) 157, 280, 388, 682, 1255, 1423, 2617
BstMAI GTCTC 4 cut(s) 194, 808, 1146, 2248
BstMBI GATC 7 cut(s) 154, 277, 385, 679, 1252, 1420, 2614
BstMCI CGRYCG 3 cut(s) 280, 307, 2773
BstMWI GCNNNNNNNGC 7 cut(s) 279, 303, 1055, 1661, 2117, 2277, 2775
BstNI CCWGG 6 cut(s) 930, 1257, 2171, 2245, 2495, 2701
BstNSI RCATGY 3 cut(s) 1362, 1724, 1986
BstSCI CCNGG 6 cut(s) 928, 1255, 2169, 2243, 2493, 2699
BstSLI GKGCMC 1 cut(s) 2341
BstSNI TACGTA 1 cut(s) 337
BstUI CGCG 2 cut(s) 1097, 2631
BstV2I GAAGAC 1 cut(s) 1633
BstX2I RGATCY 2 cut(s) 385, 1420
BstYI RGATCY 2 cut(s) 385, 1420
BstZ17I GTATAC 1 cut(s) 726
BstZI CGGCCG 2 cut(s) 304, 2770
Bsu15I ATCGAT 1 cut(s) 1941
Bsu36I CCTNAGG 1 cut(s) 1404
BsuI GTATCC 3 cut(s) 1310, 1798, 2072
BsuRI GGCC 5 cut(s) 306, 328, 1818, 2395, 2772
BsuTUI ATCGAT 1 cut(s) 1941
BtgZI GCGATG 3 cut(s) 672, 1464, 2387
BtrI CACGTC 1 cut(s) 818
BtsI GCAGTG 2 cut(s) 172, 2694
BtsIMutI CAGTG 6 cut(s) 172, 1012, 2339, 2369, 2694, 2805
BveI ACCTGC 1 cut(s) 539
Cac8I GCNNGC 5 cut(s) 2118, 2135, 2408, 2629, 2736
CaiI CAGNNNCTG 2 cut(s) 174, 511
CciI TCATGA 1 cut(s) 1633
CciNI GCGGCCGC 1 cut(s) 304
CfoI GCGC 2 cut(s) 1097, 2633
Cfr10I RCCGGY 2 cut(s) 269, 1518
Cfr13I GGNCC 3 cut(s) 327, 451, 2821
ClaI ATCGAT 1 cut(s) 1941
CpoI CGGWCCG 1 cut(s) 451
CseI GACGC 2 cut(s) 211, 1524
CsiI ACCWGGT 1 cut(s) 2243
Csp6I GTAC 9 cut(s) 926, 960, 1550, 1724, 1787, 2142, 2326, 2373, 2620
CspI CGGWCCG 1 cut(s) 451
CviQI GTAC 9 cut(s) 926, 960, 1550, 1724, 1787, 2142, 2326, 2373, 2620
DdeI CTNAG 7 cut(s) 651, 749, 1169, 1312, 1349, 1404, 2292
DpnI GATC 7 cut(s) 156, 279, 387, 681, 1254, 1422, 2616
DpnII GATC 7 cut(s) 154, 277, 385, 679, 1252, 1420, 2614
DraI TTTAAA 1 cut(s) 15
DraIII CACNNNGTG 1 cut(s) 1619
DriI GACNNNNNGTC 1 cut(s) 2473
EaeI YGGCCR 3 cut(s) 304, 1816, 2770
EagI CGGCCG 2 cut(s) 304, 2770
Eam1104I CTCTTC 6 cut(s) 39, 98, 402, 1897, 2097, 2270
Eam1105I GACNNNNNGTC 1 cut(s) 2473
EarI CTCTTC 6 cut(s) 39, 98, 402, 1897, 2097, 2270
Ecl136II GAGCTC 2 cut(s) 297, 2627
EclXI CGGCCG 2 cut(s) 304, 2770
Eco105I TACGTA 1 cut(s) 337
Eco130I CCWWGG 3 cut(s) 1282, 1731, 1809
Eco24I GRGCYC 3 cut(s) 34, 299, 2629
Eco32I GATATC 1 cut(s) 1400
Eco47I GGWCC 2 cut(s) 451, 2821
Eco52I CGGCCG 2 cut(s) 304, 2770
Eco53kI GAGCTC 2 cut(s) 297, 2627
Eco57I CTGAAG 4 cut(s) 446, 1080, 1249, 1689
Eco72I CACGTG 1 cut(s) 1616
Eco81I CCTNAGG 1 cut(s) 1404
EcoICRI GAGCTC 2 cut(s) 297, 2627
EcoO109I RGGNCCY 1 cut(s) 2821
EcoRI GAATTC 1 cut(s) 444
EcoRII CCWGG 6 cut(s) 928, 1255, 2169, 2243, 2493, 2699
EcoRV GATATC 1 cut(s) 1400
EcoT14I CCWWGG 3 cut(s) 1282, 1731, 1809
EcoT22I ATGCAT 1 cut(s) 1706
EcoT38I GRGCYC 3 cut(s) 34, 299, 2629
ErhI CCWWGG 3 cut(s) 1282, 1731, 1809
Esp3I CGTCTC 2 cut(s) 194, 808
FalI AAGNNNNNCTT 4 cut(s) 1592, 1624, 2100, 2132
FaqI GGGAC 3 cut(s) 964, 2477, 2807
FauI CCCGC 3 cut(s) 450, 2454, 2520
FbaI TGATCA 1 cut(s) 154
FblI GTMKAC 1 cut(s) 725
FriOI GRGCYC 3 cut(s) 34, 299, 2629
FspBI CTAG 8 cut(s) 21, 350, 564, 600, 1041, 1137, 1661, 2321
GlaI GCGC 2 cut(s) 1096, 2632
GsaI CCCAGC 1 cut(s) 1380
GsuI CTGGAG 3 cut(s) 151, 988, 2153
HaeIII GGCC 5 cut(s) 306, 328, 1818, 2395, 2772
HapII CCGG 8 cut(s) 270, 300, 325, 418, 449, 454, 1504, 1519
HgaI GACGC 2 cut(s) 211, 1524
HhaI GCGC 2 cut(s) 1097, 2633
Hin1I GRCGYC 2 cut(s) 183, 1516
Hin6I GCGC 2 cut(s) 1095, 2631
HinP1I GCGC 2 cut(s) 1095, 2631
HindIII AAGCTT 2 cut(s) 578, 2408
HpaII CCGG 8 cut(s) 270, 300, 325, 418, 449, 454, 1504, 1519
HphI GGTGA 8 cut(s) 106, 214, 259, 2206, 2273, 2369, 2435, 2558
Hpy166II GTNNAC 8 cut(s) 726, 960, 1339, 1789, 2326, 2339, 2359, 2443
Hpy188III TCNNGA 8 cut(s) 152, 168, 813, 848, 1293, 1403, 1634, 2253
Hpy8I GTNNAC 8 cut(s) 726, 960, 1339, 1789, 2326, 2339, 2359, 2443
Hpy99I CGWCG 3 cut(s) 185, 188, 1563
HpyAV CCTTC 6 cut(s) 959, 1055, 1072, 1664, 2293, 2792
HpyCH4III ACNGT 4 cut(s) 845, 1769, 2501, 2809
HpyCH4IV ACGT 7 cut(s) 183, 336, 712, 817, 1615, 1879, 2082
HpyF10VI GCNNNNNNNGC 7 cut(s) 279, 303, 1055, 1661, 2117, 2277, 2775
HpyF3I CTNAG 7 cut(s) 651, 749, 1169, 1312, 1349, 1404, 2292
HpySE526I ACGT 7 cut(s) 183, 336, 712, 817, 1615, 1879, 2082
Hsp92I GRCGYC 2 cut(s) 183, 1516
HspAI GCGC 2 cut(s) 1095, 2631
Ksp22I TGATCA 1 cut(s) 154
Kzo9I GATC 7 cut(s) 154, 277, 385, 679, 1252, 1420, 2614
LguI GCTCTTC 2 cut(s) 39, 2270
LmnI GCTCC 4 cut(s) 302, 1233, 2040, 2172
LweI GCATC 3 cut(s) 1934, 2443, 2787
MabI ACCWGGT 1 cut(s) 2243
MaeI CTAG 8 cut(s) 21, 350, 564, 600, 1041, 1137, 1661, 2321
MaeII ACGT 7 cut(s) 183, 336, 712, 817, 1615, 1879, 2082
MaeIII GTNAC 5 cut(s) 260, 501, 1259, 2206, 2643
MalI GATC 7 cut(s) 156, 279, 387, 681, 1254, 1422, 2616
MboI GATC 7 cut(s) 154, 277, 385, 679, 1252, 1420, 2614
MfeI CAATTG 1 cut(s) 2049
MflI RGATCY 2 cut(s) 385, 1420
MhlI GDGCHC 6 cut(s) 34, 299, 1144, 2037, 2341, 2629
MlsI TGGCCA 1 cut(s) 1818
MluNI TGGCCA 1 cut(s) 1818
MlyI GAGTC 2 cut(s) 406, 946
MmeI TCCRAC 7 cut(s) 898, 1023, 1129, 1444, 1541, 1975, 2260
Mox20I TGGCCA 1 cut(s) 1818
Mph1103I ATGCAT 1 cut(s) 1706
MscI TGGCCA 1 cut(s) 1818
MseI TTAA 7 cut(s) 14, 435, 576, 582, 1749, 1776, 2175
MslI CAYNNNNRTG 3 cut(s) 1439, 1981, 2610
Msp20I TGGCCA 1 cut(s) 1818
MspA1I CMGCKG 1 cut(s) 2224
MspCI CTTAAG 1 cut(s) 575
MspI CCGG 8 cut(s) 270, 300, 325, 418, 449, 454, 1504, 1519
MspR9I CCNGG 6 cut(s) 930, 1257, 2171, 2245, 2495, 2701
MunI CAATTG 1 cut(s) 2049
Mva1269I GAATGC 1 cut(s) 2800
MvaI CCWGG 6 cut(s) 930, 1257, 2171, 2245, 2495, 2701
MvnI CGCG 2 cut(s) 1097, 2631
MwoI GCNNNNNNNGC 7 cut(s) 279, 303, 1055, 1661, 2117, 2277, 2775
NdeII GATC 7 cut(s) 154, 277, 385, 679, 1252, 1420, 2614
NlaIV GGNNCC 5 cut(s) 218, 1280, 2168, 2581, 2823
NmuCI GTSAC 1 cut(s) 501
NotI GCGGCCGC 1 cut(s) 304
NsiI ATGCAT 1 cut(s) 1706
NspI RCATGY 3 cut(s) 1362, 1724, 1986
PagI TCATGA 1 cut(s) 1633
PciI ACATGT 1 cut(s) 1358
PciSI GCTCTTC 2 cut(s) 39, 2270
PctI GAATGC 1 cut(s) 2800
PflFI GACNNNGTC 1 cut(s) 2245
PflMI CCANNNNNTGG 1 cut(s) 2700
PfoI TCCNGGA 1 cut(s) 2493
Ple19I CGATCG 1 cut(s) 280
PleI GAGTC 2 cut(s) 406, 946
PmaCI CACGTG 1 cut(s) 1616
PmlI CACGTG 1 cut(s) 1616
PpsI GAGTC 2 cut(s) 406, 946
Ppu21I YACGTR 3 cut(s) 337, 1616, 2083
PpuMI RGGWCCY 1 cut(s) 2821
PscI ACATGT 1 cut(s) 1358
Psp124BI GAGCTC 2 cut(s) 299, 2629
Psp5II RGGWCCY 1 cut(s) 2821
Psp6I CCWGG 6 cut(s) 928, 1255, 2169, 2243, 2493, 2699
PspCI CACGTG 1 cut(s) 1616
PspFI CCCAGC 1 cut(s) 1376
PspGI CCWGG 6 cut(s) 928, 1255, 2169, 2243, 2493, 2699
PspN4I GGNNCC 5 cut(s) 218, 1280, 2168, 2581, 2823
PspPI GGNCC 3 cut(s) 327, 451, 2821
PspPPI RGGWCCY 1 cut(s) 2821
PstNI CAGNNNCTG 2 cut(s) 174, 511
PsuI RGATCY 2 cut(s) 385, 1420
PsyI GACNNNGTC 1 cut(s) 2245
PvuI CGATCG 1 cut(s) 280
PvuII CAGCTG 1 cut(s) 2224
RgaI GCGATCGC 1 cut(s) 280
RsaI GTAC 9 cut(s) 927, 961, 1551, 1725, 1788, 2143, 2327, 2374, 2621
RsaNI GTAC 9 cut(s) 926, 960, 1550, 1724, 1787, 2142, 2326, 2373, 2620
RseI CAYNNNNRTG 3 cut(s) 1439, 1981, 2610
Rsr2I CGGWCCG 1 cut(s) 451
RsrII CGGWCCG 1 cut(s) 451
SacI GAGCTC 2 cut(s) 299, 2629
SapI GCTCTTC 2 cut(s) 39, 2270
SaqAI TTAA 7 cut(s) 14, 435, 576, 582, 1749, 1776, 2175
Sau3AI GATC 7 cut(s) 154, 277, 385, 679, 1252, 1420, 2614
Sau96I GGNCC 3 cut(s) 327, 451, 2821
ScaI AGTACT 2 cut(s) 2143, 2374
SchI GAGTC 2 cut(s) 406, 946
ScrFI CCNGG 6 cut(s) 930, 1257, 2171, 2245, 2495, 2701
SduI GDGCHC 6 cut(s) 34, 299, 1144, 2037, 2341, 2629
SexAI ACCWGGT 1 cut(s) 2243
SfaAI GCGATCGC 1 cut(s) 280
SfaNI GCATC 3 cut(s) 1934, 2443, 2787
SgfI GCGATCGC 1 cut(s) 280
SgrAI CRCCGGYG 1 cut(s) 269
SinI GGWCC 2 cut(s) 451, 2821
SmiMI CAYNNNNRTG 3 cut(s) 1439, 1981, 2610
SmlI CTYRAG 4 cut(s) 575, 848, 1174, 2471
SmoI CTYRAG 4 cut(s) 575, 848, 1174, 2471
SnaBI TACGTA 1 cut(s) 337
SpeI ACTAGT 2 cut(s) 1136, 2320
SsiI CCGC 9 cut(s) 273, 303, 307, 457, 1097, 1364, 2447, 2513, 2769
SspI AATATT 3 cut(s) 1331, 2520, 2793
SspMI CTAG 8 cut(s) 21, 350, 564, 600, 1041, 1137, 1661, 2321
SstI GAGCTC 2 cut(s) 299, 2629
StyD4I CCNGG 6 cut(s) 928, 1255, 2169, 2243, 2493, 2699
StyI CCWWGG 3 cut(s) 1282, 1731, 1809
TaaI ACNGT 4 cut(s) 845, 1769, 2501, 2809
TaiI ACGT 7 cut(s) 186, 339, 715, 820, 1618, 1882, 2085
TaqI TCGA 7 cut(s) 343, 410, 812, 1745, 1895, 1941, 2252
TatI WGTACW 6 cut(s) 959, 1786, 2141, 2325, 2372, 2619
TauI GCSGC 4 cut(s) 276, 306, 309, 2772
Tru1I TTAA 7 cut(s) 14, 435, 576, 582, 1749, 1776, 2175
Tru9I TTAA 7 cut(s) 14, 435, 576, 582, 1749, 1776, 2175
TscAI CASTG 6 cut(s) 179, 1012, 2346, 2369, 2701, 2812
TseFI GTSAC 1 cut(s) 501
Tsp45I GTSAC 1 cut(s) 501
TspRI CASTG 6 cut(s) 179, 1012, 2346, 2369, 2701, 2812
Tth111I GACNNNGTC 1 cut(s) 2245
Van91I CCANNNNNTGG 1 cut(s) 2700
Vha464I CTTAAG 1 cut(s) 575
VneI GTGCAC 1 cut(s) 2337
VpaK11BI GGWCC 2 cut(s) 451, 2821
XapI RAATTY 6 cut(s) 444, 799, 1117, 1227, 1427, 1741
XceI RCATGY 3 cut(s) 1362, 1724, 1986
XmiI GTMKAC 1 cut(s) 725
XspI CTAG 8 cut(s) 21, 350, 564, 600, 1041, 1137, 1661, 2321
ZraI GACGTC 1 cut(s) 184
ZrmI AGTACT 2 cut(s) 2143, 2374
Zsp2I ATGCAT 1 cut(s) 1706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.