FvH4_3g20280

Protein of unknown function (DUF2921)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000420
Physical Location & Seq
Reverse (-)
13324628 .. 13327337
2710 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g20280.t1

Sequence Viewer

Length: 2598 bp
ATGTCCAGGGAGTATAAGTTTTCTGCTACCACCTCTGTAAATATGTGGTACACAGTGCCAAAGCCTCTCATATTCCTCTTTGTTTTCACCGCATCTTTGAACTCATTTTCATCTTCTGCTATACCTGATATACTGTCTTATGCTGATCATTGTGCTTCAACTGTTCCTGAATCAATTCCAATAAGATATGCCAGTTTTGATCCGTCTGTCCTTAGCCATACAGGTTATTATGCTGCTGGTGGCAGTAGCAGTGGCATTCGTAGTCCAAAGAGAGGATATCAACACAATCAAGAGCCATCATATCCAGTTGAGTTTGATGTCTGGAGTGTTGAAGAAACAGATGTACAAGACTTACTTGAGGTTCACGGAAGTCTTCAATTTCAAAGAGACACTGACCACATGGGAACCCTTAAAAACCGCCTGCCTCAAAGTCCTATAAGATTTGCACTGAATGGTTTCTGGTCATCATCTTCTGGAAAGCTTTGCATGGCTGGATCAGGTACTATCTACTCAAACCAAGGTGACCTGCTTAGTGATGTTCCTGCTCTTCTCAAGTTGCATAATCTCATGAATTTCACTAGTGTTACGAGTTTGATTAGTGGAACCCTGGAGAGCTTGATGAGTTCTCAGAAACAACCCAATTATTTTGAACCAGTCTCAGTTTTGATGCTTCCTCGTATGAACTATCAGAGCAGACCAGGATATTATCTTCAGTACTCGAGCCACTGCCTTTCTGCCAAGAACTGCACTACTCCTCTTTCTGGGTCTGCTTATTTCCCTAGCATTGTTGCATTAAGGGGCATTGAATGTTTGGAAGATAATCGGAGGTTGAGAGTTCTGGTAGAATTTGCAGACCAGAGAAAACACTGGTATGAGAAGAATTTCAATCCCAATACGACTTTGGTTGGGGAGGGATCATGGGATGTAAACAAAACTCAGCTACAAGTTGTTGCCTGTCATTTCTTGGATGCCACAAATTCATTCAACAACTCTCATGTAGGTGATTGCTCTACAAGACTGACCTTGACGTTTCCTGGAATATGGACTATCAGAGATACCAGAGAGGCTGCAGGACACATTTGGAGCAACAAAGCTGTGGTAGAGTCGGGTTACTTTGAAAATATTACATTTGGTAGTCTTATGAATTATGATGCAGTCTATGAACCTGCAGGTCTGAAATATGAGTATACCCAAATTGAGAAGGTAACCAAGCTGTGCCCCAGAGAGAAGAGCACTGCTCCCACTGATTACAAAACCAAGGTATACCCAAATCCCTTTCTATATGACATGAGGTTCCAAATTTTTTTCAAAAGATCTAAAAGCGAAGTGGATCGGGGCAGGTCTTATCCTCTTTCTGCAGGAAATTGGTTTTATAAATGGGGACATTATATTGAAGATGCACACTCACTTGCCTTTAACCAGCGGGGCCCCTATAATGTTAGCTTCATTGTATTCATCAATGTGAGTAATGTATCAATTGTAGATGTGATATCTGCTGAAGGGGTCTATGATGATACAGAAGGAAGCATGTGCATGGTAGGCTGCCGAAATATAGGCCCAATAAATCAACAGTCAACTGATGATTCTGTTGATTGTGAGATTTTACTCAAACTTCAGTTACCTCCAGCAACTCCAGAGAGGATGATCTCAGGTTATGTCAAGGGGAGCATTGAAAGCACACGGAAAAAGTCTGATCCTCTTCATTTTGAACGTTTGGATTTGTCTTCAGATGACATGTTCATGGCTGAAGCAGATCGATCCATTCATAGGATGGAGGTGGAGACAACCTTGGTTCTTATATCCACCGCACTTGCATATGTTTTTGGGGCATTACAACTCTTCCATGTGAAGAAACGTCCTGATATGCTTCCCTCAATTTCACTACTGATGCTGCTAATTCTAACCCTTGGGTACTTGAAACCTCTCATATATTACTTCAATGCCATCTTCAACTATAGCACCAACCACCAGGGCATGTTCATTGGAAGTGATGGATGGCTTCAAATTGATCAGGCATATCTGATGGCAATTACAGTTATGACAATGTTAGCTTTCTTGTTGCAATTCCGCCTTTTTCAGCAAACATGGTCAGCAAGATCTTCGAAAGATACCTTGAAGGAGCTTTGGGATGCGGAGAAGAAGGCTGTATCAGTATATGCGATTGGGATTTTAGCTGCTATGGCGCTTCTGATGATTTCAAGTCTAGAGTATCCCATTTTGGATTCAAGTAACTATGGTGCTTATGCTGGTTTGGTGTTGGATGGCTTTCTGGTTCCTCAAATTCTACTCAACATGGTCTGCAAATCAAAGGGAAAAGCTTTGTCAGCTGGATTCTACATCGGAACAACCATCATTCGAATCCTGCCACACGCATATGATCTTTACAGGACTGGCAGCTCTGATTTGGTCTTGGAAGAGCCATATGTTTTTGCAAGTCCTGTGGCTGGATTTTACTCCACTACATGGGATATCATTATTCCTATGGGAGCTCTACTGTTTTCTATGATCATCTTCTTGCAGCAGAAGTTTGGGGGTCGTTGCTTTCTTCCTCAAAAGTTGAGGGAGTTGGGTGATAAGAAGGTTCCAAAATGTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
DUF2921

Protein Analysis

866

Amino Acids

97.27

Weight (kDa)

6.43

Isoelectric Point (pI)

43.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2921_N PF25333 47 - 220 2.4e-19 DUF2921 lipocalin-like domain
DUF2921_N PF25333 234 - 375 3.4e-21 DUF2921 lipocalin-like domain
DUF2921_N PF25333 403 - 573 1.5e-26 DUF2921 lipocalin-like domain
DUF2921 PF11145 584 - 853 9.3e-55 Transmembrane E3 ligase/DUF2921 transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21700
fragaria_vesca FvH4_1g10360 FvH4_1g10760 FvH4_2g30370 FvH4_3g09240 FvH4_3g20050 FvH4_3g20280 FvH4_3g20290 FvH4_3g20291 FvH4_3g20340 FvH4_3g20350 FvH4_3g20351 FvH4_3g20380 FvH4_3g20390 FvH4_5g13190 FvH4_6g20070 FvH4_6g42890
malus_domestica MD03G1227000.v1.1 MD10G1294900.v1.1 MD11G1246300.v1.1 MD11G1246400.v1.1 MD11G1246500.v1.1 MD11G1246600.v1.1
prunus_persica Prupe.4G047300_v2.0.a1 Prupe.4G181700_v2.0.a1 Prupe.4G181900_v2.0.a1 Prupe.4G182000_v2.0.a1 Prupe.4G182200_v2.0.a1
pyrus_communis pycom03g17470 pycom10g24670 pycom11g21640
rosa_chinensis RchiOBHm_Chr5g0008381 RchiOBHm_Chr5g0008391 RchiOBHm_Chr5g0033881 RchiOBHm_Chr5g0034171 RchiOBHm_Chr5g0034181 RchiOBHm_Chr5g0034191 RchiOBHm_Chr5g0034201 RchiOBHm_Chr5g0034211 RchiOBHm_Chr5g0034221 RchiOBHm_Chr5g0034231 RchiOBHm_Chr5g0034271 RchiOBHm_Chr5g0034281
rosa_laevigata RLG00000031566 RLG00000031567 RLG00000033506 RLG00000033529 RLG00000033530 RLG00000033531
rosa_multiflora Rmu_co8445509.1_g000001 Rmu_co8470615.1_g000001 Rmu_sc0008805.1_g000003 Rmu_sc0008805.1_g000004 Rmu_ssc0000128.1_g000029 Rmu_ssc0000128.1_g000031 Rmu_ssc0000128.1_g000032 Rmu_ssc0000388.1_g000062 Rmu_ssc0000388.1_g000063
rosa_roxburghii Rroxscaffold_1G00046010 Rroxscaffold_1G00046020 Rroxscaffold_1G00046030 Rroxscaffold_1G00046040 Rroxscaffold_1G00046060 Rroxscaffold_1G00046070 Rroxscaffold_1G00046080 Rroxscaffold_1G00046090
rosa_rugosa Rorug04G0435500 Rorug05G0142900 Rorug05G0144600 Rorug05G0144600 Rorug05G0144700 Rorug05G0144800
rosa_samantha Rh5AG233800 Rh5AG235900 Rh5AG236000 Rh5AG236100 Rh5AG236200 Rh5AG236300 Rh5AG236500 Rh5BG061200 Rh5BG061300 Rh5BG234300 Rh5BG236600 Rh5BG236800 Rh5CG071200 Rh5DG060000 Rh5DG060100 Rh5DG241300 Rh5DG243700 Rh5DG244000 Rh5DG244100
rosa_wichuraiana Rw3G018950 Rw5G005680 Rw5G021330 Rw5G021340 Rw5G021350 Rw5G021550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1374
Acc36I ACCTGC 4 cut(s) 534, 1160, 1174, 1329
AccB7I CCANNNNNTGG 2 cut(s) 2463, 2591
AccI GTMKAC 2 cut(s) 1187, 1263
AciI CCGC 6 cut(s) 90, 418, 1423, 1806, 2068, 2132
AclI AACGTT 1 cut(s) 1711
AclWI GGATC 6 cut(s) 194, 502, 922, 1338, 1688, 1752
AcsI RAATTY 6 cut(s) 571, 845, 880, 976, 1299, 2280
AcuI CTGAAG 5 cut(s) 695, 1518, 1598, 1710, 1767
AfaI GTAC 5 cut(s) 50, 345, 502, 716, 1913
AfiI CCNNNNNNNGG 6 cut(s) 272, 761, 1767, 2444, 2463, 2591
AflIII ACRYGT 1 cut(s) 1734
AhlI ACTAGT 1 cut(s) 578
AjnI CCWGG 5 cut(s) 5, 606, 697, 1033, 1968
Alw21I GWGCWC 2 cut(s) 1235, 2491
Alw26I GTCTC 3 cut(s) 381, 661, 1775
AlwI GGATC 6 cut(s) 194, 502, 922, 1338, 1688, 1752
Ama87I CYCGRG 1 cut(s) 718
AoxI GGCC 2 cut(s) 1426, 1555
ApaI GGGCCC 1 cut(s) 1430
ApeKI GCWGC 7 cut(s) 233, 1067, 1542, 1891, 2174, 2394, 2518
ApoI RAATTY 6 cut(s) 571, 845, 880, 976, 1299, 2280
Asp700I GAANNNNTTC 3 cut(s) 174, 455, 881
AspLEI GCGC 1 cut(s) 2185
AspS9I GGNCC 3 cut(s) 1426, 1427, 1556
AsuHPI GGTGA 4 cut(s) 79, 533, 1013, 2582
AsuII TTCGAA 2 cut(s) 2102, 2356
AvaI CYCGRG 1 cut(s) 718
BaeGI GKGCMC 2 cut(s) 1220, 1430
BanII GRGCYC 2 cut(s) 1430, 2491
BbsI GAAGAC 2 cut(s) 365, 1716
Bbv12I GWGCWC 2 cut(s) 1235, 2491
BbvI GCAGC 7 cut(s) 220, 1054, 1529, 1878, 2161, 2406, 2530
BccI CCATC 8 cut(s) 304, 1765, 1952, 1985, 1989, 2017, 2255, 2357
BcgI CGANNNNNNTGC 2 cut(s) 2082, 2116
BciT130I CCWGG 5 cut(s) 7, 608, 699, 1035, 1970
BciVI GTATCC 1 cut(s) 2220
BclI TGATCA 3 cut(s) 145, 2008, 2505
BcoDI GTCTC 3 cut(s) 381, 661, 1775
BcuI ACTAGT 1 cut(s) 578
BfaI CTAG 3 cut(s) 579, 780, 2204
BfmI CTRYAG 4 cut(s) 1068, 1167, 1356, 1954
BfoI RGCGCY 1 cut(s) 2186
BfuAI ACCTGC 4 cut(s) 534, 1160, 1174, 1329
BfuI GTATCC 1 cut(s) 2220
BglII AGATCT 2 cut(s) 1313, 2096
BisI GCNGC 7 cut(s) 234, 1068, 1543, 1892, 2175, 2395, 2519
BlsI GCNGC 7 cut(s) 235, 1069, 1544, 1893, 2176, 2396, 2520
BmcAI AGTACT 1 cut(s) 716
Bme1390I CCNGG 5 cut(s) 7, 608, 699, 1035, 1970
BmeT110I CYCGRG 1 cut(s) 718
BmgT120I GGNCC 3 cut(s) 1426, 1427, 1556
BmiI GGNNCC 8 cut(s) 406, 604, 1295, 1427, 1428, 1429, 2274, 2583
BmrFI CCNGG 5 cut(s) 7, 608, 699, 1035, 1970
BmsI GCATC 7 cut(s) 101, 657, 958, 1141, 1387, 1878, 2119
BpiI GAAGAC 2 cut(s) 365, 1716
BplI GAGNNNNNCTC 2 cut(s) 1222, 1254
BpmI CTGGAG 4 cut(s) 343, 629, 1608, 1617
Bpu10I CCTNAGC 1 cut(s) 212
Bpu14I TTCGAA 2 cut(s) 2102, 2356
BpuEI CTTGAG 2 cut(s) 377, 536
Bsa29I ATCGAT 1 cut(s) 1756
BsaJI CCNNGG 7 cut(s) 6, 517, 606, 1257, 1788, 1906, 1969
BsaXI ACNNNNNCTCC 1 cut(s) 32
Bsc4I CCNNNNNNNGG 6 cut(s) 272, 761, 1767, 2444, 2463, 2591
Bse1I ACTGG 5 cut(s) 192, 305, 653, 872, 2395
BseBI CCWGG 5 cut(s) 7, 608, 699, 1035, 1970
BseCI ATCGAT 1 cut(s) 1756
BseDI CCNNGG 7 cut(s) 6, 517, 606, 1257, 1788, 1906, 1969
BseGI GGATG 7 cut(s) 928, 973, 1647, 1776, 2000, 2134, 2266
BseLI CCNNNNNNNGG 6 cut(s) 272, 761, 1767, 2444, 2463, 2591
BseMII CTCAG 4 cut(s) 641, 672, 950, 1662
BseNI ACTGG 5 cut(s) 192, 305, 653, 872, 2395
BseRI GAGGAG 1 cut(s) 744
BseSI GKGCMC 2 cut(s) 1220, 1430
BseXI GCAGC 7 cut(s) 220, 1054, 1529, 1878, 2161, 2406, 2530
BsgI GTGCAG 1 cut(s) 730
BshFI GGCC 2 cut(s) 1428, 1557
BshVI ATCGAT 1 cut(s) 1756
BsiHKAI GWGCWC 2 cut(s) 1235, 2491
BsiHKCI CYCGRG 1 cut(s) 718
BslFI GGGAC 1 cut(s) 1395
BslI CCNNNNNNNGG 6 cut(s) 272, 761, 1767, 2444, 2463, 2591
BsmAI GTCTC 3 cut(s) 381, 661, 1775
BsmFI GGGAC 1 cut(s) 1395
BsmI GAATGC 1 cut(s) 255
BsnI GGCC 2 cut(s) 1428, 1557
BsoBI CYCGRG 1 cut(s) 718
Bsp119I TTCGAA 2 cut(s) 2102, 2356
Bsp120I GGGCCC 1 cut(s) 1426
Bsp1286I GDGCHC 4 cut(s) 1220, 1235, 1430, 2491
Bsp1407I TGTACA 1 cut(s) 343
BspACI CCGC 6 cut(s) 90, 418, 1423, 1806, 2068, 2132
BspANI GGCC 2 cut(s) 1428, 1557
BspCNI CTCAG 4 cut(s) 640, 671, 949, 1661
BspDI ATCGAT 1 cut(s) 1756
BspHI TCATGA 1 cut(s) 567
BspLI GGNNCC 8 cut(s) 406, 604, 1295, 1427, 1428, 1429, 2274, 2583
BspMAI CTGCAG 3 cut(s) 1072, 1171, 1360
BspMI ACCTGC 4 cut(s) 534, 1160, 1174, 1329
BspPI GGATC 6 cut(s) 194, 502, 922, 1338, 1688, 1752
BspQI GCTCTTC 3 cut(s) 552, 1223, 2409
BspT104I TTCGAA 2 cut(s) 2102, 2356
BsrGI TGTACA 1 cut(s) 343
BsrI ACTGG 5 cut(s) 192, 305, 653, 872, 2395
BssECI CCNNGG 7 cut(s) 6, 517, 606, 1257, 1788, 1906, 1969
BssNAI GTATAC 2 cut(s) 1188, 1264
BssT1I CCWWGG 4 cut(s) 517, 1257, 1788, 1906
Bst1107I GTATAC 2 cut(s) 1188, 1264
Bst2UI CCWGG 5 cut(s) 7, 608, 699, 1035, 1970
Bst4CI ACNGT 6 cut(s) 55, 135, 163, 1572, 2035, 2496
Bst6I CTCTTC 5 cut(s) 552, 1223, 1704, 1844, 2409
BstAUI TGTACA 1 cut(s) 343
BstBI TTCGAA 2 cut(s) 2102, 2356
BstC8I GCNNGC 1 cut(s) 422
BstDEI CTNAG 6 cut(s) 212, 530, 627, 658, 936, 1648
BstEII GGTNACC 2 cut(s) 521, 1204
BstF5I GGATG 7 cut(s) 928, 973, 1647, 1776, 2000, 2134, 2266
BstH2I RGCGCY 1 cut(s) 2186
BstHHI GCGC 1 cut(s) 2185
BstMAI GTCTC 3 cut(s) 381, 661, 1775
BstMWI GCNNNNNNNGC 4 cut(s) 1539, 1674, 2180, 2324
BstNI CCWGG 5 cut(s) 7, 608, 699, 1035, 1970
BstNSI RCATGY 3 cut(s) 1531, 1738, 1978
BstPI GGTNACC 2 cut(s) 521, 1204
BstSCI CCNGG 5 cut(s) 5, 606, 697, 1033, 1968
BstSFI CTRYAG 4 cut(s) 1068, 1167, 1356, 1954
BstSLI GKGCMC 2 cut(s) 1220, 1430
BstV1I GCAGC 7 cut(s) 220, 1054, 1529, 1878, 2161, 2406, 2530
BstV2I GAAGAC 2 cut(s) 365, 1716
BstX2I RGATCY 2 cut(s) 1313, 2096
BstYI RGATCY 2 cut(s) 1313, 2096
BstZ17I GTATAC 2 cut(s) 1188, 1264
Bsu15I ATCGAT 1 cut(s) 1756
BsuI GTATCC 1 cut(s) 2220
BsuRI GGCC 2 cut(s) 1428, 1557
BsuTUI ATCGAT 1 cut(s) 1756
BtsCI GGATG 7 cut(s) 928, 973, 1647, 1776, 2000, 2134, 2266
BtsI GCAGTG 3 cut(s) 256, 724, 1233
BtsIMutI CAGTG 8 cut(s) 60, 256, 390, 446, 724, 865, 1233, 1242
BveI ACCTGC 4 cut(s) 534, 1160, 1174, 1329
Cac8I GCNNGC 1 cut(s) 422
CciI TCATGA 1 cut(s) 567
CfoI GCGC 1 cut(s) 2185
Cfr13I GGNCC 3 cut(s) 1426, 1427, 1556
ClaI ATCGAT 1 cut(s) 1756
Csp6I GTAC 5 cut(s) 49, 344, 501, 715, 1912
CspCI CAANNNNNGTGG 4 cut(s) 1792, 1827, 2421, 2456
CviQI GTAC 5 cut(s) 49, 344, 501, 715, 1912
DdeI CTNAG 6 cut(s) 212, 530, 627, 658, 936, 1648
Eam1104I CTCTTC 5 cut(s) 552, 1223, 1704, 1844, 2409
EarI CTCTTC 5 cut(s) 552, 1223, 1704, 1844, 2409
EciI GGCGGA 1 cut(s) 2057
Ecl136II GAGCTC 1 cut(s) 2489
Eco130I CCWWGG 4 cut(s) 517, 1257, 1788, 1906
Eco24I GRGCYC 2 cut(s) 1430, 2491
Eco32I GATATC 3 cut(s) 278, 1491, 2470
Eco53kI GAGCTC 1 cut(s) 2489
Eco57I CTGAAG 5 cut(s) 695, 1518, 1598, 1710, 1767
Eco88I CYCGRG 1 cut(s) 718
Eco91I GGTNACC 2 cut(s) 521, 1204
EcoICRI GAGCTC 1 cut(s) 2489
EcoO109I RGGNCCY 2 cut(s) 1426, 1427
EcoO65I GGTNACC 2 cut(s) 521, 1204
EcoRII CCWGG 5 cut(s) 5, 606, 697, 1033, 1968
EcoRV GATATC 3 cut(s) 278, 1491, 2470
EcoT14I CCWWGG 4 cut(s) 517, 1257, 1788, 1906
EcoT38I GRGCYC 2 cut(s) 1430, 2491
ErhI CCWWGG 4 cut(s) 517, 1257, 1788, 1906
FalI AAGNNNNNCTT 2 cut(s) 339, 371
FaqI GGGAC 1 cut(s) 1395
FauI CCCGC 1 cut(s) 1416
FauNDI CATATG 3 cut(s) 1816, 2374, 2422
FbaI TGATCA 3 cut(s) 145, 2008, 2505
FblI GTMKAC 2 cut(s) 1187, 1263
Fnu4HI GCNGC 7 cut(s) 234, 1068, 1543, 1892, 2175, 2395, 2519
FokI GGATG 7 cut(s) 935, 980, 1654, 1783, 2007, 2141, 2273
FriOI GRGCYC 2 cut(s) 1430, 2491
Fsp4HI GCNGC 7 cut(s) 234, 1068, 1543, 1892, 2175, 2395, 2519
FspBI CTAG 3 cut(s) 579, 780, 2204
GlaI GCGC 1 cut(s) 2184
GluI GCNGC 7 cut(s) 234, 1068, 1543, 1892, 2175, 2395, 2519
GsuI CTGGAG 4 cut(s) 343, 629, 1608, 1617
HaeII RGCGCY 1 cut(s) 2186
HaeIII GGCC 2 cut(s) 1428, 1557
HhaI GCGC 1 cut(s) 2185
Hin6I GCGC 1 cut(s) 2183
HinP1I GCGC 1 cut(s) 2183
HincII GTYRAC 1 cut(s) 1575
HindII GTYRAC 1 cut(s) 1575
HindIII AAGCTT 2 cut(s) 479, 2316
HinfI GANTC 6 cut(s) 170, 1103, 1583, 2222, 2331, 2358
HphI GGTGA 4 cut(s) 79, 533, 1013, 2582
Hpy166II GTNNAC 6 cut(s) 51, 364, 928, 1188, 1264, 1575
Hpy188III TCNNGA 8 cut(s) 167, 290, 322, 474, 568, 1634, 1859, 2204
Hpy8I GTNNAC 6 cut(s) 51, 364, 928, 1188, 1264, 1575
HpyAV CCTTC 6 cut(s) 1195, 1493, 1514, 2110, 2134, 2572
HpyCH4III ACNGT 6 cut(s) 55, 135, 163, 1572, 2035, 2496
HpyCH4IV ACGT 3 cut(s) 1028, 1711, 1855
HpyF10VI GCNNNNNNNGC 4 cut(s) 1539, 1674, 2180, 2324
HpyF3I CTNAG 6 cut(s) 212, 530, 627, 658, 936, 1648
HpySE526I ACGT 3 cut(s) 1028, 1711, 1855
HspAI GCGC 1 cut(s) 2183
Ksp22I TGATCA 3 cut(s) 145, 2008, 2505
LguI GCTCTTC 3 cut(s) 552, 1223, 2409
LmnI GCTCC 5 cut(s) 1083, 1243, 1665, 2119, 2486
Lsp1109I GCAGC 7 cut(s) 220, 1054, 1529, 1878, 2161, 2406, 2530
LweI GCATC 7 cut(s) 101, 657, 958, 1141, 1387, 1878, 2119
MaeI CTAG 3 cut(s) 579, 780, 2204
MaeII ACGT 3 cut(s) 1028, 1711, 1855
MaeIII GTNAC 6 cut(s) 521, 583, 1109, 1204, 1617, 2228
MfeI CAATTG 1 cut(s) 1476
MflI RGATCY 2 cut(s) 1313, 2096
MhlI GDGCHC 4 cut(s) 1220, 1235, 1430, 2491
MlyI GAGTC 1 cut(s) 1112
MmeI TCCRAC 1 cut(s) 2238
MroXI GAANNNNTTC 3 cut(s) 174, 455, 881
MseI TTAA 3 cut(s) 411, 794, 1416
MslI CAYNNNNRTG 6 cut(s) 870, 999, 1460, 1532, 1739, 2373
MspA1I CMGCKG 2 cut(s) 1423, 2327
MspR9I CCNGG 5 cut(s) 7, 608, 699, 1035, 1970
MunI CAATTG 1 cut(s) 1476
Mva1269I GAATGC 1 cut(s) 255
MvaI CCWGG 5 cut(s) 7, 608, 699, 1035, 1970
MwoI GCNNNNNNNGC 4 cut(s) 1539, 1674, 2180, 2324
NdeI CATATG 3 cut(s) 1816, 2374, 2422
NlaIV GGNNCC 8 cut(s) 406, 604, 1295, 1427, 1428, 1429, 2274, 2583
NmuCI GTSAC 1 cut(s) 521
NspI RCATGY 3 cut(s) 1531, 1738, 1978
NspV TTCGAA 2 cut(s) 2102, 2356
PaeR7I CTCGAG 1 cut(s) 718
PagI TCATGA 1 cut(s) 567
PciI ACATGT 1 cut(s) 1734
PciSI GCTCTTC 3 cut(s) 552, 1223, 2409
PctI GAATGC 1 cut(s) 255
PdmI GAANNNNTTC 3 cut(s) 174, 455, 881
PfeI GAWTC 5 cut(s) 170, 1583, 2222, 2331, 2358
PflMI CCANNNNNTGG 2 cut(s) 2463, 2591
PfoI TCCNGGA 1 cut(s) 1033
PkrI GCNGC 7 cut(s) 235, 1069, 1544, 1893, 2176, 2396, 2520
PleI GAGTC 1 cut(s) 1111
PpsI GAGTC 1 cut(s) 1111
PscI ACATGT 1 cut(s) 1734
PsiI TTATAA 1 cut(s) 1374
Psp124BI GAGCTC 1 cut(s) 2491
Psp1406I AACGTT 1 cut(s) 1711
Psp6I CCWGG 5 cut(s) 5, 606, 697, 1033, 1968
PspEI GGTNACC 2 cut(s) 521, 1204
PspGI CCWGG 5 cut(s) 5, 606, 697, 1033, 1968
PspN4I GGNNCC 8 cut(s) 406, 604, 1295, 1427, 1428, 1429, 2274, 2583
PspOMI GGGCCC 1 cut(s) 1426
PspPI GGNCC 3 cut(s) 1426, 1427, 1556
PspXI VCTCGAGB 1 cut(s) 718
PstI CTGCAG 3 cut(s) 1072, 1171, 1360
PsuI RGATCY 2 cut(s) 1313, 2096
PvuII CAGCTG 1 cut(s) 2327
RsaI GTAC 5 cut(s) 50, 345, 502, 716, 1913
RsaNI GTAC 5 cut(s) 49, 344, 501, 715, 1912
RseI CAYNNNNRTG 6 cut(s) 870, 999, 1460, 1532, 1739, 2373
SacI GAGCTC 1 cut(s) 2491
SapI GCTCTTC 3 cut(s) 552, 1223, 2409
SaqAI TTAA 3 cut(s) 411, 794, 1416
SatI GCNGC 7 cut(s) 234, 1068, 1543, 1892, 2175, 2395, 2519
Sau96I GGNCC 3 cut(s) 1426, 1427, 1556
SbfI CCTGCAGG 1 cut(s) 1171
ScaI AGTACT 1 cut(s) 716
SchI GAGTC 1 cut(s) 1112
ScrFI CCNGG 5 cut(s) 7, 608, 699, 1035, 1970
SdaI CCTGCAGG 1 cut(s) 1171
SduI GDGCHC 4 cut(s) 1220, 1235, 1430, 2491
SfaNI GCATC 7 cut(s) 101, 657, 958, 1141, 1387, 1878, 2119
SfcI CTRYAG 4 cut(s) 1068, 1167, 1356, 1954
Sfr274I CTCGAG 1 cut(s) 718
SfuI TTCGAA 2 cut(s) 2102, 2356
SlaI CTCGAG 1 cut(s) 718
SmiMI CAYNNNNRTG 6 cut(s) 870, 999, 1460, 1532, 1739, 2373
SmlI CTYRAG 3 cut(s) 356, 551, 718
SmoI CTYRAG 3 cut(s) 356, 551, 718
SpeI ACTAGT 1 cut(s) 578
Sse8387I CCTGCAGG 1 cut(s) 1171
SsiI CCGC 6 cut(s) 90, 418, 1423, 1806, 2068, 2132
SspI AATATT 1 cut(s) 1123
SspMI CTAG 3 cut(s) 579, 780, 2204
SstI GAGCTC 1 cut(s) 2491
StyD4I CCNGG 5 cut(s) 5, 606, 697, 1033, 1968
StyI CCWWGG 4 cut(s) 517, 1257, 1788, 1906
TaaI ACNGT 6 cut(s) 55, 135, 163, 1572, 2035, 2496
TaiI ACGT 3 cut(s) 1031, 1714, 1858
TaqI TCGA 4 cut(s) 719, 1756, 2102, 2356
TatI WGTACW 2 cut(s) 343, 714
TfiI GAWTC 5 cut(s) 170, 1583, 2222, 2331, 2358
Tru1I TTAA 3 cut(s) 411, 794, 1416
Tru9I TTAA 3 cut(s) 411, 794, 1416
TscAI CASTG 8 cut(s) 60, 256, 397, 453, 731, 872, 1240, 1249
TseFI GTSAC 1 cut(s) 521
TseI GCWGC 7 cut(s) 233, 1067, 1542, 1891, 2174, 2394, 2518
Tsp45I GTSAC 1 cut(s) 521
TspGWI ACGGA 3 cut(s) 192, 381, 1696
TspRI CASTG 8 cut(s) 60, 256, 397, 453, 731, 872, 1240, 1249
Van91I CCANNNNNTGG 2 cut(s) 2463, 2591
XapI RAATTY 6 cut(s) 571, 845, 880, 976, 1299, 2280
XbaI TCTAGA 1 cut(s) 2203
XceI RCATGY 3 cut(s) 1531, 1738, 1978
XcmI CCANNNNNNNNNTGG 2 cut(s) 898, 1768
XhoI CTCGAG 1 cut(s) 718
XmiI GTMKAC 2 cut(s) 1187, 1263
XmnI GAANNNNTTC 3 cut(s) 174, 455, 881
XspI CTAG 3 cut(s) 579, 780, 2204
ZrmI AGTACT 1 cut(s) 716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.