Rw5G005680

Protein of unknown function (DUF2921)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000420
Physical Location & Seq
Forward (+)
6004163 .. 6007024
2862 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G005680.1

Sequence Viewer

Length: 2862 bp
ATGGAGACCAATTTAAAATCTAGGGTTAGGGCTCTTCCAATTTTCCCAACCACTCCTCCACAAACCCATAAATCCCTACTCACTCCCCTCCTCCTCCTCCTCTTCTTCTTCTTCTTCATCAATTTCACCCCCACAGCTTCAACCCCGAACCAAATCCCCAATCTTGATCAACTATACTCCAAACACTGCAACGACGTCGTTCCCAAATCGGAGACGCACCCCAAGTGGTTCCTCACCACCCTCTCCATCCAAGACCTCGGATTCCACAGCAGTTACTTCACCGGCGGCGATCGCCTTTTCAATCAGAGCTCCGGCGGCCGCAAAGCTCTCATCTTCCGGCCCAACTATGTAGCTCGAACCCTAGCGGATGGAATTTTCCATGTCAAAGGAAGTATGGATCTTCAATCTCTTCTGGGCTATTCGACTCGCCGGAATCTGAAGTTGGTTAAGCCCAGAATTCCGGTCCGGCGGGGCGTAGTGAGGTTTAGGCTTGATGGGTATTATTCAGAATCGTCAAAGAAGCTGTGTATGATTGGGAAAGTTATGTCCATGTATAATGCAGGTAAGTTTGAGGCTAGTGTTGTTCTTAAGCTTAATTATCCGAAATGGTCTAGTGTTTATGATAGTTTGATTACTGGGAGTTTAGAGAGCGTTGTTATTGCTGAGGGCGATGATGGTAAGAATTATTTTGATCCCGTTTTGATGTTGGGTTTGTCTGTGAAACGTAGTTATGAGTATACATTTGTTGGGAAAGATAGGCTGAGTAGTTTTCCGAGTAGTGTTGATAGAGGAGAGAGTATAGCTTTGGAGAATTTGAATGATTCGAGACGTGACTTGTGTAGGTTGCTTGGTAACCGTCTTGAGAGATATGAATTGGTGTATGGGAGTGAGTGTGGGGCTGTGAATTGCAATCCTCTTGGTGGGGATGTTGGATATGTACCCGGCTCGTTGGTTTTTTATGGGACTCGGTGTACAGAAGGGAGGAAAGTGCAGATGTTGTTGGGGTTTTCCAACTCCAGTGTAGATGAATACATAAGATTTCCATTTGTGCCTAGCACGACGTTCATTGCTGAAGGGGAATGGGATGAGAAGGGGAATCGTCTATGCGCGGTTGCATGTAGGATTTTGAATTTTACAGAATCTTTGACTAGTGCTCGTGTTGGAGACTGCTCAACTAAACTCAGCTTGAGATTTCCAGTAAAGTTGTCTTTACAAAATAGGTCTGCTGTTGTGGGGCAAATTTGGAGCGACAAAAAAGTGAGTGATCCTGGTTACTTCAGTAAAATTGGGTTCCAAGGTTTGTCGGGATGGTTAGTGAAACTCTTAGGATACAAATATGAATATTCTGTGAACGACAACCTGAGAAAAACATGTGCGGAAAAGAGGGCTGGGAGAGGCAAAGGAAAGCGATATCCTGAGGAGTATTCATCAGATCTGAAATTTGACATGAGAGTGAGCAACAGCAAAGGACAAGTTGCATGGGGTTATTCATCGCCATTATTTGTTGATGATGACCGGATTTATGGACGCCGGTTTTGGGATAAACCACAGGAAACAGAAGTACCAAGTCGTCGGAAGAAAAGCCATAGCAGTCCGATGAACATTACCTATAAGTTGAGCTTCACACGTGGTGTTCAATTTTTTCATGATGTCTTCCCATCTAAAGCAGAACTAGCTTCAGAAGGAATATATGATAGAGATTATGGAAACCTATGCATGATAGGATGTAGGCATGTACCATCCAAGGAACAAAATTCGATTAAAAAGGATATGTTAGACTGTTCTGTTAAAATTGATGTACACTTTCCTCCATTGGATACCAAGGATGGCCAGAATGTCAAGGGAACTATTGAAAGCACGAGGAATAAGTCAGACCCTCTTTATTTTGAACGTATTGACTTCTCTTCGACTTCAATTTACCATAACCAAGCTGCTGCATCCATTACGAGAATCGATTTTGAGATTGTTATGGTTTTGATTTCCAATACACTTGCATGTGCTTTTGTCGGATGGCAGCTCTATTTTGTGAAGAAAAACCCAGATGTGCTCCCATTCACTTCAATTGTCATGCTCATTGTTCTTACTCTGGGATACGTGATTCCTCTTCTGTTCAACTTTGAAGCAATGCTTGCACCAAACCAAAGCAAGCAGAGTACTTTTCTTGGGATTGGTGGATGGCTCCAGGTTAATGAAGTAATGGTCAGGGTGATAATGTTGGTAACTTTCATTTTGCAGCTGCTTCTTCTCCAACTGACCTGGTCATCGAGACAAGGTGATAATAACCAGAAGAGCTTGATGGATTCTGAGAGGAAGGTTCTTTATGCCACCTTACTATTGTACACAGCTGGTGCACTGATTACTTGCTCTGTCCACCAGTGGACGAGTACTCACCAGAACTCATACAGGCCATTTCATCGCAAGCTTCGGCGAGTGGCTAACCTATGGCATCTTGTTCACCCGCAACGCTCTCTCTGGGACGACCTCAAGTCTTATGCTGGATTCATCCTGGACGGTTTTCTACTCCCGCAAATATTGTTCAACTTATTCGTCAATTCAGGTGAAAAGGCTCTGTCCTGTGCCTTTTACTTTGGAACCACCATGATTCGTCTGCTGCCTCATGCTTATGATCTGTACAGAGCTCGCGCTTACACATGGTTACTTGCTTTCTCATATATTTATGCAAACCCCAGAACCGATTTTTTCTCCACTGCCTGGAACATAATCATACCCTGTTGTTGTCTGCTGCTTGCTGCCATTATTTTCTTGCAGCAAAGGTTTGGCGGCCGTTGCATCCTTCCCAAAATATCTGGGCACTCCACTGTATATGAAAAGGTCCCTGTCATCAGCAATGAGGAATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
DUF2921

Protein Analysis

953

Amino Acids

108.24

Weight (kDa)

9.28

Isoelectric Point (pI)

38.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2921_N PF25333 59 - 233 2.1e-18 DUF2921 lipocalin-like domain
DUF2921_N PF25333 259 - 429 1.3e-30 DUF2921 lipocalin-like domain
DUF2921_N PF25333 460 - 633 1.5e-32 DUF2921 lipocalin-like domain
DUF2921 PF11145 644 - 935 4.1e-59 Transmembrane E3 ligase/DUF2921 transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21700
fragaria_vesca FvH4_1g10360 FvH4_1g10760 FvH4_2g30370 FvH4_3g09240 FvH4_3g20050 FvH4_3g20280 FvH4_3g20290 FvH4_3g20291 FvH4_3g20340 FvH4_3g20350 FvH4_3g20351 FvH4_3g20380 FvH4_3g20390 FvH4_5g13190 FvH4_6g20070 FvH4_6g42890
malus_domestica MD03G1227000.v1.1 MD10G1294900.v1.1 MD11G1246300.v1.1 MD11G1246400.v1.1 MD11G1246500.v1.1 MD11G1246600.v1.1
prunus_persica Prupe.4G047300_v2.0.a1 Prupe.4G181700_v2.0.a1 Prupe.4G181900_v2.0.a1 Prupe.4G182000_v2.0.a1 Prupe.4G182200_v2.0.a1
pyrus_communis pycom03g17470 pycom10g24670 pycom11g21640
rosa_chinensis RchiOBHm_Chr5g0008381 RchiOBHm_Chr5g0008391 RchiOBHm_Chr5g0033881 RchiOBHm_Chr5g0034171 RchiOBHm_Chr5g0034181 RchiOBHm_Chr5g0034191 RchiOBHm_Chr5g0034201 RchiOBHm_Chr5g0034211 RchiOBHm_Chr5g0034221 RchiOBHm_Chr5g0034231 RchiOBHm_Chr5g0034271 RchiOBHm_Chr5g0034281
rosa_laevigata RLG00000031566 RLG00000031567 RLG00000033506 RLG00000033529 RLG00000033530 RLG00000033531
rosa_multiflora Rmu_co8445509.1_g000001 Rmu_co8470615.1_g000001 Rmu_sc0008805.1_g000003 Rmu_sc0008805.1_g000004 Rmu_ssc0000128.1_g000029 Rmu_ssc0000128.1_g000031 Rmu_ssc0000128.1_g000032 Rmu_ssc0000388.1_g000062 Rmu_ssc0000388.1_g000063
rosa_roxburghii Rroxscaffold_1G00046010 Rroxscaffold_1G00046020 Rroxscaffold_1G00046030 Rroxscaffold_1G00046040 Rroxscaffold_1G00046060 Rroxscaffold_1G00046070 Rroxscaffold_1G00046080 Rroxscaffold_1G00046090
rosa_rugosa Rorug04G0435500 Rorug05G0142900 Rorug05G0144600 Rorug05G0144600 Rorug05G0144700 Rorug05G0144800
rosa_samantha Rh5AG233800 Rh5AG235900 Rh5AG236000 Rh5AG236100 Rh5AG236200 Rh5AG236300 Rh5AG236500 Rh5BG061200 Rh5BG061300 Rh5BG234300 Rh5BG236600 Rh5BG236800 Rh5CG071200 Rh5DG060000 Rh5DG060100 Rh5DG241300 Rh5DG243700 Rh5DG244000 Rh5DG244100
rosa_wichuraiana Rw3G018950 Rw5G005680 Rw5G021330 Rw5G021340 Rw5G021350 Rw5G021550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 198
Acc36I ACCTGC 1 cut(s) 551
AccB7I CCANNNNNTGG 1 cut(s) 2712
AccI GTMKAC 1 cut(s) 737
AccII CGCG 2 cut(s) 1109, 2643
AclWI GGATC 3 cut(s) 405, 686, 1259
AcoI YGGCCR 3 cut(s) 316, 1828, 2782
AcsI RAATTY 7 cut(s) 372, 456, 811, 1129, 1239, 1439, 1753
AcuI CTGAAG 4 cut(s) 458, 1092, 1261, 1662
AcvI CACGTG 1 cut(s) 1628
AcyI GRCGYC 2 cut(s) 195, 1528
AdeI CACNNNGTG 1 cut(s) 1631
AfaI GTAC 9 cut(s) 939, 973, 1563, 1737, 1800, 2155, 2339, 2386, 2633
AfiI CCNNNNNNNGG 3 cut(s) 920, 1537, 2712
AflII CTTAAG 1 cut(s) 587
AflIII ACRYGT 2 cut(s) 1370, 1625
AhdI GACNNNNNGTC 1 cut(s) 2485
AhlI ACTAGT 1 cut(s) 1148
AjiI CACGTC 1 cut(s) 830
AjnI CCWGG 5 cut(s) 1267, 2181, 2255, 2505, 2711
AjuI GAANNNNNNNTTGG 2 cut(s) 425, 457
Alw21I GWGCWC 5 cut(s) 311, 1156, 2049, 2353, 2641
Alw26I GTCTC 4 cut(s) 206, 820, 1158, 2260
Alw44I GTGCAC 1 cut(s) 2349
AlwI GGATC 3 cut(s) 405, 686, 1259
AoxI GGCC 5 cut(s) 316, 338, 1828, 2405, 2782
ApaLI GTGCAC 1 cut(s) 2349
ApeKI GCWGC 9 cut(s) 1931, 1934, 2014, 2233, 2236, 2611, 2743, 2750, 2767
ApoI RAATTY 7 cut(s) 372, 456, 811, 1129, 1239, 1439, 1753
ArsI GACNNNNNNTTYG 2 cut(s) 1518, 1550
AsiSI GCGATCGC 1 cut(s) 292
AspLEI GCGC 2 cut(s) 1109, 2645
AspS9I GGNCC 3 cut(s) 339, 463, 2833
AsuC2I CCSGG 1 cut(s) 942
AsuHPI GGTGA 8 cut(s) 118, 226, 271, 2218, 2285, 2381, 2447, 2570
AvaII GGWCC 2 cut(s) 463, 2833
AxyI CCTNAGG 1 cut(s) 1416
BaeGI GKGCMC 2 cut(s) 2353, 2814
BaeI ACNNNNGTAYC 2 cut(s) 1545, 1578
BalI TGGCCA 1 cut(s) 1830
BanII GRGCYC 3 cut(s) 34, 311, 2641
BauI CACGAG 2 cut(s) 1155, 1858
BbrPI CACGTG 1 cut(s) 1628
BbsI GAAGAC 1 cut(s) 1645
Bbv12I GWGCWC 5 cut(s) 311, 1156, 2049, 2353, 2641
BbvCI CCTCAGC 1 cut(s) 663
BbvI GCAGC 9 cut(s) 1918, 1921, 2026, 2223, 2245, 2598, 2730, 2737, 2779
BceAI ACGGC 1 cut(s) 2769
BcgI CGANNNNNNTGC 2 cut(s) 178, 212
BciT130I CCWGG 5 cut(s) 1269, 2183, 2257, 2507, 2713
BciVI GTATCC 3 cut(s) 1322, 1810, 2084
BclI TGATCA 1 cut(s) 166
BcnI CCSGG 1 cut(s) 942
BcoDI GTCTC 4 cut(s) 206, 820, 1158, 2260
BcuI ACTAGT 1 cut(s) 1148
BfaI CTAG 7 cut(s) 21, 362, 576, 612, 1053, 1149, 1673
BfrI CTTAAG 1 cut(s) 587
BfuAI ACCTGC 1 cut(s) 551
BfuI GTATCC 3 cut(s) 1322, 1810, 2084
BglII AGATCT 1 cut(s) 1432
BmcAI AGTACT 2 cut(s) 2155, 2386
Bme1390I CCNGG 6 cut(s) 942, 1269, 2183, 2257, 2507, 2713
Bme18I GGWCC 2 cut(s) 463, 2833
BmeRI GACNNNNNGTC 1 cut(s) 2485
BmgBI CACGTC 1 cut(s) 830
BmgT120I GGNCC 3 cut(s) 339, 463, 2833
BmiI GGNNCC 5 cut(s) 230, 1292, 2180, 2593, 2835
BmrFI CCNGG 6 cut(s) 942, 1269, 2183, 2257, 2507, 2713
BmrI ACTGGG 1 cut(s) 645
BmsI GCATC 3 cut(s) 1946, 2455, 2799
BmuI ACTGGG 1 cut(s) 645
BpiI GAAGAC 1 cut(s) 1645
BpmI CTGGAG 2 cut(s) 1000, 2165
Bpu10I CCTNAGC 1 cut(s) 663
BpuEI CTTGAG 3 cut(s) 881, 1207, 2468
BpuMI CCSGG 1 cut(s) 942
Bsa29I ATCGAT 1 cut(s) 1953
BsaAI YACGTR 2 cut(s) 1628, 2095
BsaHI GRCGYC 2 cut(s) 195, 1528
BsaJI CCNNGG 4 cut(s) 256, 1294, 1743, 1821
BsaWI WCCGGW 2 cut(s) 460, 1515
BsaXI ACNNNNNCTCC 4 cut(s) 40, 70, 877, 907
Bsc4I CCNNNNNNNGG 3 cut(s) 920, 1537, 2712
Bse118I RCCGGY 2 cut(s) 281, 1530
Bse1I ACTGG 4 cut(s) 640, 1017, 1196, 2374
Bse21I CCTNAGG 1 cut(s) 1416
Bse3DI GCAATG 3 cut(s) 1065, 2130, 2854
BseBI CCWGG 5 cut(s) 1269, 2183, 2257, 2507, 2713
BseCI ATCGAT 1 cut(s) 1953
BseDI CCNNGG 4 cut(s) 256, 1294, 1743, 1821
BseLI CCNNNNNNNGG 3 cut(s) 920, 1537, 2712
BseMI GCAATG 3 cut(s) 1065, 2130, 2854
BseMII CTCAG 6 cut(s) 654, 752, 1195, 1352, 1407, 2295
BseNI ACTGG 4 cut(s) 640, 1017, 1196, 2374
BseRI GAGGAG 7 cut(s) 45, 80, 83, 86, 89, 804, 1433
BseSI GKGCMC 2 cut(s) 2353, 2814
BseX3I CGGCCG 2 cut(s) 316, 2782
BseXI GCAGC 9 cut(s) 1918, 1921, 2026, 2223, 2245, 2598, 2730, 2737, 2779
BseYI CCCAGC 1 cut(s) 1388
BsgI GTGCAG 1 cut(s) 1010
Bsh1236I CGCG 2 cut(s) 1109, 2643
Bsh1285I CGRYCG 3 cut(s) 292, 319, 2785
BshFI GGCC 5 cut(s) 318, 340, 1830, 2407, 2784
BshVI ATCGAT 1 cut(s) 1953
BsiEI CGRYCG 3 cut(s) 292, 319, 2785
BsiHKAI GWGCWC 5 cut(s) 311, 1156, 2049, 2353, 2641
BsiSI CCGG 9 cut(s) 282, 312, 337, 430, 461, 466, 942, 1516, 1531
BslFI GGGAC 3 cut(s) 976, 2489, 2819
BslI CCNNNNNNNGG 3 cut(s) 920, 1537, 2712
BsmAI GTCTC 4 cut(s) 206, 820, 1158, 2260
BsmBI CGTCTC 2 cut(s) 206, 820
BsmFI GGGAC 3 cut(s) 976, 2489, 2819
BsnI GGCC 5 cut(s) 318, 340, 1830, 2407, 2784
Bsp1286I GDGCHC 7 cut(s) 34, 311, 1156, 2049, 2353, 2641, 2814
Bsp1407I TGTACA 4 cut(s) 971, 1798, 2337, 2631
Bsp143I GATC 7 cut(s) 166, 289, 397, 691, 1264, 1432, 2626
BspANI GGCC 5 cut(s) 318, 340, 1830, 2407, 2784
BspCNI CTCAG 6 cut(s) 655, 753, 1194, 1353, 1408, 2296
BspDI ATCGAT 1 cut(s) 1953
BspFNI CGCG 2 cut(s) 1109, 2643
BspHI TCATGA 1 cut(s) 1645
BspLI GGNNCC 5 cut(s) 230, 1292, 2180, 2593, 2835
BspMI ACCTGC 1 cut(s) 551
BspPI GGATC 3 cut(s) 405, 686, 1259
BspQI GCTCTTC 2 cut(s) 39, 2282
BspTI CTTAAG 1 cut(s) 587
BsrDI GCAATG 3 cut(s) 1065, 2130, 2854
BsrFI RCCGGY 2 cut(s) 281, 1530
BsrGI TGTACA 4 cut(s) 971, 1798, 2337, 2631
BsrI ACTGG 4 cut(s) 640, 1017, 1196, 2374
BssAI RCCGGY 2 cut(s) 281, 1530
BssECI CCNNGG 4 cut(s) 256, 1294, 1743, 1821
BssMI GATC 7 cut(s) 166, 289, 397, 691, 1264, 1432, 2626
BssNAI GTATAC 1 cut(s) 738
BssNI GRCGYC 2 cut(s) 195, 1528
BssSI CACGAG 2 cut(s) 1155, 1858
BssT1I CCWWGG 3 cut(s) 1294, 1743, 1821
Bst1107I GTATAC 1 cut(s) 738
Bst2BI CACGAG 2 cut(s) 1155, 1858
Bst2UI CCWGG 5 cut(s) 1269, 2183, 2257, 2507, 2713
Bst4CI ACNGT 4 cut(s) 857, 1781, 2513, 2821
Bst6I CTCTTC 6 cut(s) 39, 107, 414, 1909, 2109, 2282
BstACI GRCGYC 2 cut(s) 195, 1528
BstAFI CTTAAG 1 cut(s) 587
BstAPI GCANNNNNTGC 1 cut(s) 2129
BstAUI TGTACA 4 cut(s) 971, 1798, 2337, 2631
BstBAI YACGTR 2 cut(s) 1628, 2095
BstC8I GCNNGC 5 cut(s) 2130, 2147, 2420, 2641, 2748
BstDEI CTNAG 7 cut(s) 663, 761, 1181, 1324, 1361, 1416, 2304
BstEII GGTNACC 1 cut(s) 851
BstFNI CGCG 2 cut(s) 1109, 2643
BstHHI GCGC 2 cut(s) 1109, 2645
BstKTI GATC 7 cut(s) 169, 292, 400, 694, 1267, 1435, 2629
BstMAI GTCTC 4 cut(s) 206, 820, 1158, 2260
BstMBI GATC 7 cut(s) 166, 289, 397, 691, 1264, 1432, 2626
BstMCI CGRYCG 3 cut(s) 292, 319, 2785
BstMWI GCNNNNNNNGC 5 cut(s) 291, 315, 1673, 2129, 2787
BstNI CCWGG 5 cut(s) 1269, 2183, 2257, 2507, 2713
BstNSI RCATGY 4 cut(s) 1119, 1374, 1736, 1998
BstPI GGTNACC 1 cut(s) 851
BstSCI CCNGG 6 cut(s) 940, 1267, 2181, 2255, 2505, 2711
BstSLI GKGCMC 2 cut(s) 2353, 2814
BstUI CGCG 2 cut(s) 1109, 2643
BstV1I GCAGC 9 cut(s) 1918, 1921, 2026, 2223, 2245, 2598, 2730, 2737, 2779
BstV2I GAAGAC 1 cut(s) 1645
BstX2I RGATCY 2 cut(s) 397, 1432
BstYI RGATCY 2 cut(s) 397, 1432
BstZ17I GTATAC 1 cut(s) 738
BstZI CGGCCG 2 cut(s) 316, 2782
Bsu15I ATCGAT 1 cut(s) 1953
Bsu36I CCTNAGG 1 cut(s) 1416
BsuI GTATCC 3 cut(s) 1322, 1810, 2084
BsuRI GGCC 5 cut(s) 318, 340, 1830, 2407, 2784
BsuTUI ATCGAT 1 cut(s) 1953
BtgZI GCGATG 3 cut(s) 684, 1476, 2399
BtrI CACGTC 1 cut(s) 830
BtsI GCAGTG 2 cut(s) 184, 2706
BtsIMutI CAGTG 6 cut(s) 184, 1024, 2351, 2381, 2706, 2817
BveI ACCTGC 1 cut(s) 551
Cac8I GCNNGC 5 cut(s) 2130, 2147, 2420, 2641, 2748
CciI TCATGA 1 cut(s) 1645
CciNI GCGGCCGC 1 cut(s) 316
CfoI GCGC 2 cut(s) 1109, 2645
Cfr10I RCCGGY 2 cut(s) 281, 1530
Cfr13I GGNCC 3 cut(s) 339, 463, 2833
ClaI ATCGAT 1 cut(s) 1953
CpoI CGGWCCG 1 cut(s) 463
CseI GACGC 2 cut(s) 223, 1536
CsiI ACCWGGT 1 cut(s) 2255
Csp6I GTAC 9 cut(s) 938, 972, 1562, 1736, 1799, 2154, 2338, 2385, 2632
CspI CGGWCCG 1 cut(s) 463
CviQI GTAC 9 cut(s) 938, 972, 1562, 1736, 1799, 2154, 2338, 2385, 2632
DdeI CTNAG 7 cut(s) 663, 761, 1181, 1324, 1361, 1416, 2304
DpnI GATC 7 cut(s) 168, 291, 399, 693, 1266, 1434, 2628
DpnII GATC 7 cut(s) 166, 289, 397, 691, 1264, 1432, 2626
DraI TTTAAA 1 cut(s) 15
DraIII CACNNNGTG 1 cut(s) 1631
DriI GACNNNNNGTC 1 cut(s) 2485
EaeI YGGCCR 3 cut(s) 316, 1828, 2782
EagI CGGCCG 2 cut(s) 316, 2782
Eam1104I CTCTTC 6 cut(s) 39, 107, 414, 1909, 2109, 2282
Eam1105I GACNNNNNGTC 1 cut(s) 2485
EarI CTCTTC 6 cut(s) 39, 107, 414, 1909, 2109, 2282
Ecl136II GAGCTC 2 cut(s) 309, 2639
EclXI CGGCCG 2 cut(s) 316, 2782
Eco130I CCWWGG 3 cut(s) 1294, 1743, 1821
Eco24I GRGCYC 3 cut(s) 34, 311, 2641
Eco32I GATATC 1 cut(s) 1412
Eco47I GGWCC 2 cut(s) 463, 2833
Eco52I CGGCCG 2 cut(s) 316, 2782
Eco53kI GAGCTC 2 cut(s) 309, 2639
Eco57I CTGAAG 4 cut(s) 458, 1092, 1261, 1662
Eco72I CACGTG 1 cut(s) 1628
Eco81I CCTNAGG 1 cut(s) 1416
Eco91I GGTNACC 1 cut(s) 851
EcoICRI GAGCTC 2 cut(s) 309, 2639
EcoO109I RGGNCCY 1 cut(s) 2833
EcoO65I GGTNACC 1 cut(s) 851
EcoRI GAATTC 1 cut(s) 456
EcoRII CCWGG 5 cut(s) 1267, 2181, 2255, 2505, 2711
EcoRV GATATC 1 cut(s) 1412
EcoT14I CCWWGG 3 cut(s) 1294, 1743, 1821
EcoT22I ATGCAT 1 cut(s) 1718
EcoT38I GRGCYC 3 cut(s) 34, 311, 2641
ErhI CCWWGG 3 cut(s) 1294, 1743, 1821
Esp3I CGTCTC 2 cut(s) 206, 820
FalI AAGNNNNNCTT 4 cut(s) 1604, 1636, 2112, 2144
FaqI GGGAC 3 cut(s) 976, 2489, 2819
FauI CCCGC 3 cut(s) 462, 2466, 2532
FbaI TGATCA 1 cut(s) 166
FblI GTMKAC 1 cut(s) 737
FriOI GRGCYC 3 cut(s) 34, 311, 2641
FspBI CTAG 7 cut(s) 21, 362, 576, 612, 1053, 1149, 1673
GlaI GCGC 2 cut(s) 1108, 2644
GsaI CCCAGC 1 cut(s) 1392
GsuI CTGGAG 2 cut(s) 1000, 2165
HaeIII GGCC 5 cut(s) 318, 340, 1830, 2407, 2784
HapII CCGG 9 cut(s) 282, 312, 337, 430, 461, 466, 942, 1516, 1531
HgaI GACGC 2 cut(s) 223, 1536
HhaI GCGC 2 cut(s) 1109, 2645
Hin1I GRCGYC 2 cut(s) 195, 1528
Hin6I GCGC 2 cut(s) 1107, 2643
HinP1I GCGC 2 cut(s) 1107, 2643
HindIII AAGCTT 2 cut(s) 590, 2420
HpaII CCGG 9 cut(s) 282, 312, 337, 430, 461, 466, 942, 1516, 1531
HphI GGTGA 8 cut(s) 118, 226, 271, 2218, 2285, 2381, 2447, 2570
Hpy166II GTNNAC 9 cut(s) 738, 972, 1351, 1801, 2340, 2351, 2371, 2379, 2455
Hpy188III TCNNGA 7 cut(s) 164, 825, 860, 1305, 1415, 1646, 2265
Hpy8I GTNNAC 9 cut(s) 738, 972, 1351, 1801, 2340, 2351, 2371, 2379, 2455
Hpy99I CGWCG 4 cut(s) 197, 200, 1063, 1575
HpyAV CCTTC 6 cut(s) 971, 1067, 1084, 1676, 2305, 2804
HpyCH4III ACNGT 4 cut(s) 857, 1781, 2513, 2821
HpyCH4IV ACGT 7 cut(s) 195, 724, 829, 1061, 1627, 1891, 2094
HpyF10VI GCNNNNNNNGC 5 cut(s) 291, 315, 1673, 2129, 2787
HpyF3I CTNAG 7 cut(s) 663, 761, 1181, 1324, 1361, 1416, 2304
HpySE526I ACGT 7 cut(s) 195, 724, 829, 1061, 1627, 1891, 2094
Hsp92I GRCGYC 2 cut(s) 195, 1528
HspAI GCGC 2 cut(s) 1107, 2643
Ksp22I TGATCA 1 cut(s) 166
Kzo9I GATC 7 cut(s) 166, 289, 397, 691, 1264, 1432, 2626
LguI GCTCTTC 2 cut(s) 39, 2282
LmnI GCTCC 4 cut(s) 314, 1245, 2052, 2184
Lsp1109I GCAGC 9 cut(s) 1918, 1921, 2026, 2223, 2245, 2598, 2730, 2737, 2779
LweI GCATC 3 cut(s) 1946, 2455, 2799
MabI ACCWGGT 1 cut(s) 2255
MaeI CTAG 7 cut(s) 21, 362, 576, 612, 1053, 1149, 1673
MaeII ACGT 7 cut(s) 195, 724, 829, 1061, 1627, 1891, 2094
MaeIII GTNAC 6 cut(s) 272, 830, 851, 1271, 2218, 2655
MalI GATC 7 cut(s) 168, 291, 399, 693, 1266, 1434, 2628
MboI GATC 7 cut(s) 166, 289, 397, 691, 1264, 1432, 2626
MfeI CAATTG 1 cut(s) 2061
MflI RGATCY 2 cut(s) 397, 1432
MhlI GDGCHC 7 cut(s) 34, 311, 1156, 2049, 2353, 2641, 2814
MlsI TGGCCA 1 cut(s) 1830
MluNI TGGCCA 1 cut(s) 1830
MlyI GAGTC 2 cut(s) 418, 958
MmeI TCCRAC 6 cut(s) 910, 1035, 1141, 1553, 1987, 2272
Mox20I TGGCCA 1 cut(s) 1830
Mph1103I ATGCAT 1 cut(s) 1718
MscI TGGCCA 1 cut(s) 1830
MseI TTAA 7 cut(s) 14, 447, 588, 594, 1761, 1788, 2187
MslI CAYNNNNRTG 3 cut(s) 1451, 1993, 2622
Msp20I TGGCCA 1 cut(s) 1830
MspA1I CMGCKG 2 cut(s) 2236, 2345
MspCI CTTAAG 1 cut(s) 587
MspI CCGG 9 cut(s) 282, 312, 337, 430, 461, 466, 942, 1516, 1531
MspR9I CCNGG 6 cut(s) 942, 1269, 2183, 2257, 2507, 2713
MunI CAATTG 1 cut(s) 2061
MvaI CCWGG 5 cut(s) 1269, 2183, 2257, 2507, 2713
MvnI CGCG 2 cut(s) 1109, 2643
MwoI GCNNNNNNNGC 5 cut(s) 291, 315, 1673, 2129, 2787
NciI CCSGG 1 cut(s) 942
NdeII GATC 7 cut(s) 166, 289, 397, 691, 1264, 1432, 2626
NlaIV GGNNCC 5 cut(s) 230, 1292, 2180, 2593, 2835
NmuCI GTSAC 1 cut(s) 830
NotI GCGGCCGC 1 cut(s) 316
NsiI ATGCAT 1 cut(s) 1718
NspI RCATGY 4 cut(s) 1119, 1374, 1736, 1998
PagI TCATGA 1 cut(s) 1645
PciI ACATGT 1 cut(s) 1370
PciSI GCTCTTC 2 cut(s) 39, 2282
PflFI GACNNNGTC 1 cut(s) 2257
PflMI CCANNNNNTGG 1 cut(s) 2712
PfoI TCCNGGA 1 cut(s) 2505
Ple19I CGATCG 1 cut(s) 292
PleI GAGTC 2 cut(s) 418, 958
PmaCI CACGTG 1 cut(s) 1628
PmlI CACGTG 1 cut(s) 1628
PpsI GAGTC 2 cut(s) 418, 958
Ppu21I YACGTR 2 cut(s) 1628, 2095
PpuMI RGGWCCY 1 cut(s) 2833
PscI ACATGT 1 cut(s) 1370
Psp124BI GAGCTC 2 cut(s) 311, 2641
Psp5II RGGWCCY 1 cut(s) 2833
Psp6I CCWGG 5 cut(s) 1267, 2181, 2255, 2505, 2711
PspCI CACGTG 1 cut(s) 1628
PspEI GGTNACC 1 cut(s) 851
PspFI CCCAGC 1 cut(s) 1388
PspGI CCWGG 5 cut(s) 1267, 2181, 2255, 2505, 2711
PspN4I GGNNCC 5 cut(s) 230, 1292, 2180, 2593, 2835
PspPI GGNCC 3 cut(s) 339, 463, 2833
PspPPI RGGWCCY 1 cut(s) 2833
PsuI RGATCY 2 cut(s) 397, 1432
PsyI GACNNNGTC 1 cut(s) 2257
PvuI CGATCG 1 cut(s) 292
PvuII CAGCTG 2 cut(s) 2236, 2345
RgaI GCGATCGC 1 cut(s) 292
RsaI GTAC 9 cut(s) 939, 973, 1563, 1737, 1800, 2155, 2339, 2386, 2633
RsaNI GTAC 9 cut(s) 938, 972, 1562, 1736, 1799, 2154, 2338, 2385, 2632
RseI CAYNNNNRTG 3 cut(s) 1451, 1993, 2622
Rsr2I CGGWCCG 1 cut(s) 463
RsrII CGGWCCG 1 cut(s) 463
SacI GAGCTC 2 cut(s) 311, 2641
SapI GCTCTTC 2 cut(s) 39, 2282
SaqAI TTAA 7 cut(s) 14, 447, 588, 594, 1761, 1788, 2187
Sau3AI GATC 7 cut(s) 166, 289, 397, 691, 1264, 1432, 2626
Sau96I GGNCC 3 cut(s) 339, 463, 2833
ScaI AGTACT 2 cut(s) 2155, 2386
SchI GAGTC 2 cut(s) 418, 958
ScrFI CCNGG 6 cut(s) 942, 1269, 2183, 2257, 2507, 2713
SduI GDGCHC 7 cut(s) 34, 311, 1156, 2049, 2353, 2641, 2814
SexAI ACCWGGT 1 cut(s) 2255
SfaAI GCGATCGC 1 cut(s) 292
SfaNI GCATC 3 cut(s) 1946, 2455, 2799
SgfI GCGATCGC 1 cut(s) 292
SgrAI CRCCGGYG 1 cut(s) 281
SinI GGWCC 2 cut(s) 463, 2833
SmiMI CAYNNNNRTG 3 cut(s) 1451, 1993, 2622
SmlI CTYRAG 4 cut(s) 587, 860, 1186, 2483
SmoI CTYRAG 4 cut(s) 587, 860, 1186, 2483
SpeI ACTAGT 1 cut(s) 1148
SspI AATATT 2 cut(s) 1343, 2532
SspMI CTAG 7 cut(s) 21, 362, 576, 612, 1053, 1149, 1673
SstI GAGCTC 2 cut(s) 311, 2641
StyD4I CCNGG 6 cut(s) 940, 1267, 2181, 2255, 2505, 2711
StyI CCWWGG 3 cut(s) 1294, 1743, 1821
TaaI ACNGT 4 cut(s) 857, 1781, 2513, 2821
TaiI ACGT 7 cut(s) 198, 727, 832, 1064, 1630, 1894, 2097
TaqI TCGA 7 cut(s) 355, 422, 824, 1757, 1907, 1953, 2264
TatI WGTACW 6 cut(s) 971, 1798, 2153, 2337, 2384, 2631
TauI GCSGC 4 cut(s) 288, 318, 321, 2784
Tru1I TTAA 7 cut(s) 14, 447, 588, 594, 1761, 1788, 2187
Tru9I TTAA 7 cut(s) 14, 447, 588, 594, 1761, 1788, 2187
TscAI CASTG 6 cut(s) 191, 1024, 2358, 2381, 2713, 2824
TseFI GTSAC 1 cut(s) 830
TseI GCWGC 9 cut(s) 1931, 1934, 2014, 2233, 2236, 2611, 2743, 2750, 2767
Tsp45I GTSAC 1 cut(s) 830
TspRI CASTG 6 cut(s) 191, 1024, 2358, 2381, 2713, 2824
Tth111I GACNNNGTC 1 cut(s) 2257
Van91I CCANNNNNTGG 1 cut(s) 2712
Vha464I CTTAAG 1 cut(s) 587
VneI GTGCAC 1 cut(s) 2349
VpaK11BI GGWCC 2 cut(s) 463, 2833
XapI RAATTY 7 cut(s) 372, 456, 811, 1129, 1239, 1439, 1753
XceI RCATGY 4 cut(s) 1119, 1374, 1736, 1998
XmiI GTMKAC 1 cut(s) 737
XspI CTAG 7 cut(s) 21, 362, 576, 612, 1053, 1149, 1673
ZraI GACGTC 1 cut(s) 196
ZrmI AGTACT 2 cut(s) 2155, 2386
Zsp2I ATGCAT 1 cut(s) 1718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.