Rh5BG061300

Protein of unknown function (DUF2921)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000420
Physical Location & Seq
Reverse (-)
5051171 .. 5053459
2289 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG061300.1

Sequence Viewer

Length: 2289 bp
ATGGTTGGTACTGTTTCTCACATCAGTTCCGAGTCTCTGGTTGTTCTGAAGCTTAATTATCCCAAAAATACTAGCATTTTCGATATTTTAGTGACCGGGACTTTGGAGAGTGTTTCTGATGAGATAGATTTTGAACCCATTTCGATACTGGGGTTATCTCGGAAATCTGGTTATCAGCCCACATTCATTGGGAATGGTCGTCTGAATGGCTCTTTGGTTGGTTATGATAGAGGGGAGCGTTTAGGTATGGATAGTTTAGCTCTAGAGAAATTTGGTCGTTCGAGCCGCAGTGTATGTAGCGTTGTTGGTGGTTATGGTTTTAGAGTAGAAAGATATGAATTGGAATATGAGAGTGGTAATGGCAATCCTCTTGGTGGAGATGTTGGATATGTACCTAAGTTCTTTGTTTTTCGTAGGACTCGGTGTGAAGATGGGAAGATGCAGATGTTGTTGGGGTTTCCCAACTCGAGTTTTCATGGACGGGGTAGTACTTTTCCCTTTGAACCTAGGACAAGTTTGATTGCTGAAGGGGAATGGATTGAGAAGGAGAATCGTGTGTTAGCTGTTGCGTGCCGGATTTTGAATTTTACGGAGTCATTGACTAATGCTTTTGTGGGAGATTGTTCGACTAGACTCAGCTTTAGGTTACCTGCACGGGTGTCTTTAAGAAATAGGTCTAGTATTGTGGGGCAAATTTGGAGCAACAGAACGGTGAATGACTCTGGTTACTTTGGTAAAATTGGGTTTCACAGAGTGTCGGAACAGTTGATGAAATTCTTACCTGGTTACAAATACGAATTCACTGAGTATGACACGGTGAGGAAAACTTGTGATGAAAAGAAGACCAATGAAGGAAAAGGAAAGAAATACCCTGATGAGCATTTGTTGGAAATGAGACTTCAAATGGCAGTGAGAAACAGTACAGGGCAAACTGAATGGGTTCATGTATCCCCATTGTTTGTGGATGGTGATCGAAGTCATGCTGGGCTTTGGGATAAACAGCTGAAGAAAAGCCATAGTAGTGTGGTGAATATGAGCTATGATTTAACCTTCAGTTTCATACATGGATATAATTTCTTTGATTATGGTACCTCAGTTTCAACAAAAGTATATGCTGAAGGAATATATGATAGGGATAATGGTAATCTATGCATGATAGCATGTAGGCATGTATTGTCAAAAGATCAAGAATTGATGGAAAAGGATATGCTAGACTGTGCTATCAAAATTAATCTCCAGTTTTCTCCATTGGATGCCAAGAATCGTAGAATTGTCAATGGGACTATTGAAAGCACAAGGAGAAAGTCAGACCCTCTTTATTTTGAGCCTATGGAGTTATTATACTCGGGTTCCATTACCGAAAGGCAAGCTGCAAAGTCCATTTCAAGAATGGACTTGGAGAATGTTATGGTTCTAATTTCCAACATCCTTGCATGTGTCTTTGTTGGCTGGCAGCTCTATTATGTGAAGAAACACCCAGATGTACTCCCGTTCATCTCCATTGTGATGCTTGTTGTTCTCATTCTGGGGTACACGACTCCTCTTCTGTCCAACTTTGAAGCAATGTTTATGCGAAACCGCAACAACTGGCAGGATAAGTTTCTTGGCACTGGTGAATGGCACAAGGTGAATGAAGTAATTGTCAGCGTGATGATGTTGGTAGTTTTCCTGCTGCAGCTACGGCTTCTCCAGCTGACCTGGTGCTCGAGACAAGGTAATGGAAGCCAGAAGTGCTTGAGGGATTCCGAGAGAAAGGTTCTTTATACCACTTTACCACTATACATAGCTGGTGCATTGATTTCTTGTTTCGACCACCAGTGGATCCATACTCACCACACCTCATACATACCACTACGCATCCATTTGCCTCTTCATCTTGTTCGCCAGCGACACAATCTCTGGGATGACCTCAAGCCTTACAGTGGCTTTGTCCTGGACGGTTTCCTGCTCCCACAGATACTGTTCAACTTGTTCTTCAATTCAGGAGAAAAGGCTCTCACCTGTGCATTCTATTTCGGAACTACCATAATCCGTCTGTTGCCTCATGCATATGATCTTTACAGGGCTCATACATTTCCTCGGCTCCCTGCAGCTCTCTATTTCTTTTATGCAAACCCGGCATCGGATTTTTTCTCCACTGCCTGGAACATCATCATTCCTTGCTGTGGTCTGTTGTTTGTCACCATTGTTTTCTTGCAGCAAAGGCTTGGTGGCCGTTGCATTCTTCCCAATAAATTTAGACAGACTTCTTGTGTATATGAGAGAGTCTCTGTCATCAGCAATGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
DUF2921

Protein Analysis

762

Amino Acids

87.02

Weight (kDa)

8.83

Isoelectric Point (pI)

37.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2921_N PF25333 136 - 247 2.4e-21 DUF2921 lipocalin-like domain
DUF2921_N PF25333 275 - 444 5.2e-33 DUF2921 lipocalin-like domain
DUF2921 PF11145 457 - 746 5e-61 Transmembrane E3 ligase/DUF2921 transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21700
fragaria_vesca FvH4_1g10360 FvH4_1g10760 FvH4_2g30370 FvH4_3g09240 FvH4_3g20050 FvH4_3g20280 FvH4_3g20290 FvH4_3g20291 FvH4_3g20340 FvH4_3g20350 FvH4_3g20351 FvH4_3g20380 FvH4_3g20390 FvH4_5g13190 FvH4_6g20070 FvH4_6g42890
malus_domestica MD03G1227000.v1.1 MD10G1294900.v1.1 MD11G1246300.v1.1 MD11G1246400.v1.1 MD11G1246500.v1.1 MD11G1246600.v1.1
prunus_persica Prupe.4G047300_v2.0.a1 Prupe.4G181700_v2.0.a1 Prupe.4G181900_v2.0.a1 Prupe.4G182000_v2.0.a1 Prupe.4G182200_v2.0.a1
pyrus_communis pycom03g17470 pycom10g24670 pycom11g21640
rosa_chinensis RchiOBHm_Chr5g0008381 RchiOBHm_Chr5g0008391 RchiOBHm_Chr5g0033881 RchiOBHm_Chr5g0034171 RchiOBHm_Chr5g0034181 RchiOBHm_Chr5g0034191 RchiOBHm_Chr5g0034201 RchiOBHm_Chr5g0034211 RchiOBHm_Chr5g0034221 RchiOBHm_Chr5g0034231 RchiOBHm_Chr5g0034271 RchiOBHm_Chr5g0034281
rosa_laevigata RLG00000031566 RLG00000031567 RLG00000033506 RLG00000033529 RLG00000033530 RLG00000033531
rosa_multiflora Rmu_co8445509.1_g000001 Rmu_co8470615.1_g000001 Rmu_sc0008805.1_g000003 Rmu_sc0008805.1_g000004 Rmu_ssc0000128.1_g000029 Rmu_ssc0000128.1_g000031 Rmu_ssc0000128.1_g000032 Rmu_ssc0000388.1_g000062 Rmu_ssc0000388.1_g000063
rosa_roxburghii Rroxscaffold_1G00046010 Rroxscaffold_1G00046020 Rroxscaffold_1G00046030 Rroxscaffold_1G00046040 Rroxscaffold_1G00046060 Rroxscaffold_1G00046070 Rroxscaffold_1G00046080 Rroxscaffold_1G00046090
rosa_rugosa Rorug04G0435500 Rorug05G0142900 Rorug05G0144600 Rorug05G0144600 Rorug05G0144700 Rorug05G0144800
rosa_samantha Rh5AG233800 Rh5AG235900 Rh5AG236000 Rh5AG236100 Rh5AG236200 Rh5AG236300 Rh5AG236500 Rh5BG061200 Rh5BG061300 Rh5BG234300 Rh5BG236600 Rh5BG236800 Rh5CG071200 Rh5DG060000 Rh5DG060100 Rh5DG241300 Rh5DG243700 Rh5DG244000 Rh5DG244100
rosa_wichuraiana Rw3G018950 Rw5G005680 Rw5G021330 Rw5G021340 Rw5G021350 Rw5G021550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 658
Acc65I GGTACC 1 cut(s) 1088
AccB1I GGYRCC 1 cut(s) 1088
AccB7I CCANNNNNTGG 1 cut(s) 2142
AciI CCGC 2 cut(s) 286, 1579
AclWI GGATC 2 cut(s) 1816, 1829
AcoI YGGCCR 1 cut(s) 2212
AcsI RAATTY 6 cut(s) 269, 583, 693, 773, 797, 2234
AcuI CTGAAG 5 cut(s) 68, 546, 1025, 1036, 1137
AdeI CACNNNGTG 2 cut(s) 754, 1627
AfaI GTAC 7 cut(s) 10, 393, 490, 922, 1090, 1485, 1532
AfiI CCNNNNNNNGG 5 cut(s) 374, 1922, 2122, 2142, 2165
AjnI CCWGG 4 cut(s) 781, 1697, 1932, 2141
AjuI GAANNNNNNNTTGG 2 cut(s) 197, 229
Alw21I GWGCWC 1 cut(s) 1706
Alw26I GTCTC 4 cut(s) 39, 889, 1702, 2272
AlwI GGATC 2 cut(s) 1816, 1829
AlwNI CAGNNNCTG 1 cut(s) 1960
Ama87I CYCGRG 3 cut(s) 466, 1345, 1705
AoxI GGCC 1 cut(s) 2212
ApeKI GCWGC 6 cut(s) 1370, 1453, 1672, 1675, 2090, 2197
ApoI RAATTY 6 cut(s) 269, 583, 693, 773, 797, 2234
ArsI GACNNNNNNTTYG 2 cut(s) 85, 117
AseI ATTAAT 1 cut(s) 1230
Asp700I GAANNNNTTC 1 cut(s) 939
Asp718I GGTACC 1 cut(s) 1088
AspA2I CCTAGG 1 cut(s) 506
AsuC2I CCSGG 2 cut(s) 97, 2117
AsuHPI GGTGA 9 cut(s) 724, 829, 980, 1039, 1625, 1639, 1823, 1990, 2173
AvaI CYCGRG 3 cut(s) 466, 1345, 1705
AvrII CCTAGG 1 cut(s) 506
BaeI ACNNNNGTAYC 4 cut(s) 137, 170, 1080, 1113
BamHI GGATCC 1 cut(s) 1821
BanI GGYRCC 1 cut(s) 1088
BanII GRGCYC 1 cut(s) 2068
BbsI GAAGAC 1 cut(s) 848
Bbv12I GWGCWC 1 cut(s) 1706
BbvI GCAGC 6 cut(s) 1357, 1465, 1659, 1687, 2102, 2209
BccI CCATC 3 cut(s) 425, 959, 1189
BceAI ACGGC 2 cut(s) 1697, 2199
BciT130I CCWGG 4 cut(s) 783, 1699, 1934, 2143
BciVI GTATCC 1 cut(s) 958
BcnI CCSGG 2 cut(s) 97, 2117
BcoDI GTCTC 4 cut(s) 39, 889, 1702, 2272
BfaI CTAG 6 cut(s) 72, 263, 507, 630, 678, 1211
BfmI CTRYAG 2 cut(s) 1673, 2088
BfuAI ACCTGC 1 cut(s) 658
BfuI GTATCC 1 cut(s) 958
BisI GCNGC 7 cut(s) 286, 1371, 1454, 1673, 1676, 2091, 2198
BlnI CCTAGG 1 cut(s) 506
BlsI GCNGC 7 cut(s) 287, 1372, 1455, 1674, 1677, 2092, 2199
BmcAI AGTACT 1 cut(s) 490
Bme1390I CCNGG 6 cut(s) 97, 783, 1699, 1934, 2117, 2143
BmeT110I CYCGRG 3 cut(s) 466, 1345, 1705
BmiI GGNNCC 4 cut(s) 1090, 1351, 1823, 2084
BmrFI CCNGG 6 cut(s) 97, 783, 1699, 1934, 2117, 2143
BmrI ACTGGG 1 cut(s) 158
BmsI GCATC 5 cut(s) 429, 1243, 1497, 1866, 2129
BmuI ACTGGG 1 cut(s) 158
BpiI GAAGAC 1 cut(s) 848
BplI GAGNNNNNCTC 2 cut(s) 2252, 2284
BpmI CTGGAG 2 cut(s) 1220, 1673
BpuEI CTTGAG 2 cut(s) 1756, 1895
BpuMI CCSGG 2 cut(s) 97, 2117
BsaBI GATNNNNATC 1 cut(s) 969
BsaJI CCNNGG 2 cut(s) 506, 2078
BsaXI ACNNNNNCTCC 2 cut(s) 1671, 1701
Bsc4I CCNNNNNNNGG 5 cut(s) 374, 1922, 2122, 2142, 2165
Bse1I ACTGG 5 cut(s) 153, 1237, 1592, 1615, 1816
Bse3DI GCAATG 2 cut(s) 1569, 2287
Bse8I GATNNNNATC 1 cut(s) 969
BseBI CCWGG 4 cut(s) 783, 1699, 1934, 2143
BseDI CCNNGG 2 cut(s) 506, 2078
BseGI GGATG 5 cut(s) 970, 1258, 1425, 1857, 1909
BseJI GATNNNNATC 1 cut(s) 969
BseLI CCNNNNNNNGG 5 cut(s) 374, 1922, 2122, 2142, 2165
BseMI GCAATG 2 cut(s) 1569, 2287
BseMII CTCAG 3 cut(s) 649, 795, 1107
BseNI ACTGG 5 cut(s) 153, 1237, 1592, 1615, 1816
BseRI GAGGAG 1 cut(s) 1530
BseXI GCAGC 6 cut(s) 1357, 1465, 1659, 1687, 2102, 2209
BseYI CCCAGC 1 cut(s) 983
BsgI GTGCAG 1 cut(s) 636
BshFI GGCC 1 cut(s) 2214
BshNI GGYRCC 1 cut(s) 1088
BsiHKAI GWGCWC 1 cut(s) 1706
BsiHKCI CYCGRG 3 cut(s) 466, 1345, 1705
BsiSI CCGG 3 cut(s) 96, 574, 2117
BslFI GGGAC 2 cut(s) 112, 1294
BslI CCNNNNNNNGG 5 cut(s) 374, 1922, 2122, 2142, 2165
BsmAI GTCTC 4 cut(s) 39, 889, 1702, 2272
BsmFI GGGAC 2 cut(s) 112, 1294
BsmI GAATGC 2 cut(s) 2006, 2220
BsnI GGCC 1 cut(s) 2214
BsoBI CYCGRG 3 cut(s) 466, 1345, 1705
Bsp1286I GDGCHC 2 cut(s) 1706, 2068
Bsp143I GATC 4 cut(s) 970, 1183, 1821, 2053
BspACI CCGC 2 cut(s) 286, 1579
BspANI GGCC 1 cut(s) 2214
BspCNI CTCAG 3 cut(s) 648, 796, 1106
BspLI GGNNCC 4 cut(s) 1090, 1351, 1823, 2084
BspMAI CTGCAG 2 cut(s) 1677, 2092
BspMI ACCTGC 1 cut(s) 658
BspPI GGATC 2 cut(s) 1816, 1829
BspT107I GGYRCC 1 cut(s) 1088
BsrDI GCAATG 2 cut(s) 1569, 2287
BsrI ACTGG 5 cut(s) 153, 1237, 1592, 1615, 1816
BssECI CCNNGG 2 cut(s) 506, 2078
BssMI GATC 4 cut(s) 970, 1183, 1821, 2053
BssT1I CCWWGG 1 cut(s) 506
Bst2UI CCWGG 4 cut(s) 783, 1699, 1934, 2143
Bst4CI ACNGT 9 cut(s) 13, 712, 765, 817, 920, 1217, 1922, 1940, 1962
Bst6I CTCTTC 2 cut(s) 1548, 1875
BstC8I GCNNGC 4 cut(s) 571, 1368, 1451, 1886
BstDEI CTNAG 4 cut(s) 396, 635, 804, 1093
BstEII GGTNACC 1 cut(s) 645
BstF5I GGATG 5 cut(s) 970, 1258, 1425, 1857, 1909
BstKTI GATC 4 cut(s) 973, 1186, 1824, 2056
BstMAI GTCTC 4 cut(s) 39, 889, 1702, 2272
BstMBI GATC 4 cut(s) 970, 1183, 1821, 2053
BstMWI GCNNNNNNNGC 5 cut(s) 1681, 1690, 1731, 2117, 2203
BstNI CCWGG 4 cut(s) 783, 1699, 1934, 2143
BstNSI RCATGY 3 cut(s) 1164, 1172, 1437
BstPI GGTNACC 1 cut(s) 645
BstSCI CCNGG 6 cut(s) 95, 781, 1697, 1932, 2115, 2141
BstSFI CTRYAG 2 cut(s) 1673, 2088
BstV1I GCAGC 6 cut(s) 1357, 1465, 1659, 1687, 2102, 2209
BstV2I GAAGAC 1 cut(s) 848
BstX2I RGATCY 1 cut(s) 1821
BstYI RGATCY 1 cut(s) 1821
BsuI GTATCC 1 cut(s) 958
BsuRI GGCC 1 cut(s) 2214
BtsCI GGATG 5 cut(s) 970, 1258, 1425, 1857, 1909
BtsI GCAGTG 3 cut(s) 295, 915, 2136
BtsIMutI CAGTG 7 cut(s) 295, 801, 915, 1608, 1823, 1927, 2136
BveI ACCTGC 1 cut(s) 658
Cac8I GCNNGC 4 cut(s) 571, 1368, 1451, 1886
CaiI CAGNNNCTG 1 cut(s) 1960
CsiI ACCWGGT 2 cut(s) 781, 1697
Csp6I GTAC 7 cut(s) 9, 392, 489, 921, 1089, 1484, 1531
CspCI CAANNNNNGTGG 2 cut(s) 169, 204
CviAII CATG 9 cut(s) 476, 944, 980, 1064, 1153, 1161, 1169, 1434, 2045
CviQI GTAC 7 cut(s) 9, 392, 489, 921, 1089, 1484, 1531
DdeI CTNAG 4 cut(s) 396, 635, 804, 1093
DpnI GATC 4 cut(s) 972, 1185, 1823, 2055
DpnII GATC 4 cut(s) 970, 1183, 1821, 2053
DraIII CACNNNGTG 2 cut(s) 754, 1627
EaeI YGGCCR 1 cut(s) 2212
Eam1104I CTCTTC 2 cut(s) 1548, 1875
EarI CTCTTC 2 cut(s) 1548, 1875
Eco130I CCWWGG 1 cut(s) 506
Eco24I GRGCYC 1 cut(s) 2068
Eco57I CTGAAG 5 cut(s) 68, 546, 1025, 1036, 1137
Eco88I CYCGRG 3 cut(s) 466, 1345, 1705
Eco91I GGTNACC 1 cut(s) 645
EcoO65I GGTNACC 1 cut(s) 645
EcoRI GAATTC 1 cut(s) 797
EcoRII CCWGG 4 cut(s) 781, 1697, 1932, 2141
EcoT14I CCWWGG 1 cut(s) 506
EcoT22I ATGCAT 2 cut(s) 1154, 2050
EcoT38I GRGCYC 1 cut(s) 2068
ErhI CCWWGG 1 cut(s) 506
FaeI CATG 9 cut(s) 479, 947, 983, 1067, 1156, 1164, 1172, 1437, 2048
FaqI GGGAC 2 cut(s) 112, 1294
FatI CATG 9 cut(s) 475, 943, 979, 1063, 1152, 1160, 1168, 1433, 2044
FauNDI CATATG 1 cut(s) 2050
Fnu4HI GCNGC 7 cut(s) 286, 1371, 1454, 1673, 1676, 2091, 2198
FokI GGATG 5 cut(s) 977, 1265, 1412, 1844, 1916
FriOI GRGCYC 1 cut(s) 2068
Fsp4HI GCNGC 7 cut(s) 286, 1371, 1454, 1673, 1676, 2091, 2198
FspBI CTAG 6 cut(s) 72, 263, 507, 630, 678, 1211
GluI GCNGC 7 cut(s) 286, 1371, 1454, 1673, 1676, 2091, 2198
GsaI CCCAGC 1 cut(s) 987
GsuI CTGGAG 2 cut(s) 1220, 1673
HaeIII GGCC 1 cut(s) 2214
HapII CCGG 3 cut(s) 96, 574, 2117
Hin1II CATG 9 cut(s) 479, 947, 983, 1067, 1156, 1164, 1172, 1437, 2048
HindIII AAGCTT 1 cut(s) 50
HpaII CCGG 3 cut(s) 96, 574, 2117
HphI GGTGA 9 cut(s) 724, 829, 980, 1039, 1625, 1639, 1823, 1990, 2173
Hpy166II GTNNAC 1 cut(s) 1533
Hpy188III TCNNGA 5 cut(s) 263, 1187, 1386, 1707, 1983
Hpy8I GTNNAC 1 cut(s) 1533
HpyAV CCTTC 5 cut(s) 521, 538, 845, 1060, 1112
HpyCH4III ACNGT 9 cut(s) 13, 712, 765, 817, 920, 1217, 1922, 1940, 1962
HpyF10VI GCNNNNNNNGC 5 cut(s) 1681, 1690, 1731, 2117, 2203
HpyF3I CTNAG 4 cut(s) 396, 635, 804, 1093
Hsp92II CATG 9 cut(s) 479, 947, 983, 1067, 1156, 1164, 1172, 1437, 2048
KpnI GGTACC 1 cut(s) 1092
Kzo9I GATC 4 cut(s) 970, 1183, 1821, 2053
LmnI GCTCC 4 cut(s) 235, 699, 1953, 2088
Lsp1109I GCAGC 6 cut(s) 1357, 1465, 1659, 1687, 2102, 2209
LweI GCATC 5 cut(s) 429, 1243, 1497, 1866, 2129
MabI ACCWGGT 2 cut(s) 781, 1697
MaeI CTAG 6 cut(s) 72, 263, 507, 630, 678, 1211
MaeIII GTNAC 5 cut(s) 91, 645, 725, 785, 2179
MalI GATC 4 cut(s) 972, 1185, 1823, 2055
MboI GATC 4 cut(s) 970, 1183, 1821, 2053
MboII GAAGA 9 cut(s) 440, 448, 853, 1018, 1480, 1535, 1862, 1966, 2216
MflI RGATCY 1 cut(s) 1821
MhlI GDGCHC 2 cut(s) 1706, 2068
MlyI GAGTC 7 cut(s) 41, 412, 602, 627, 713, 1531, 2274
MmeI TCCRAC 5 cut(s) 364, 738, 867, 1446, 1575
Mph1103I ATGCAT 2 cut(s) 1154, 2050
MroXI GAANNNNTTC 1 cut(s) 939
MseI TTAA 4 cut(s) 54, 665, 1046, 1230
MslI CAYNNNNRTG 4 cut(s) 1020, 1479, 1505, 2049
MspA1I CMGCKG 2 cut(s) 1003, 1693
MspI CCGG 3 cut(s) 96, 574, 2117
MspR9I CCNGG 6 cut(s) 97, 783, 1699, 1934, 2117, 2143
Mva1269I GAATGC 2 cut(s) 2006, 2220
MvaI CCWGG 4 cut(s) 783, 1699, 1934, 2143
MwoI GCNNNNNNNGC 5 cut(s) 1681, 1690, 1731, 2117, 2203
NciI CCSGG 2 cut(s) 97, 2117
NdeI CATATG 1 cut(s) 2050
NdeII GATC 4 cut(s) 970, 1183, 1821, 2053
NlaIII CATG 9 cut(s) 479, 947, 983, 1067, 1156, 1164, 1172, 1437, 2048
NlaIV GGNNCC 4 cut(s) 1090, 1351, 1823, 2084
NmeAIII GCCGAG 1 cut(s) 2059
NmuCI GTSAC 2 cut(s) 91, 2179
NsiI ATGCAT 2 cut(s) 1154, 2050
NspI RCATGY 3 cut(s) 1164, 1172, 1437
PaeR7I CTCGAG 2 cut(s) 466, 1705
PctI GAATGC 2 cut(s) 2006, 2220
PdmI GAANNNNTTC 1 cut(s) 939
PfeI GAWTC 3 cut(s) 550, 1261, 1742
PflMI CCANNNNNTGG 1 cut(s) 2142
PfoI TCCNGGA 1 cut(s) 1932
PkrI GCNGC 7 cut(s) 287, 1372, 1455, 1674, 1677, 2092, 2199
PleI GAGTC 7 cut(s) 40, 412, 601, 627, 713, 1531, 2273
PpsI GAGTC 7 cut(s) 40, 412, 601, 627, 713, 1531, 2273
PshBI ATTAAT 1 cut(s) 1230
Psp6I CCWGG 4 cut(s) 781, 1697, 1932, 2141
PspEI GGTNACC 1 cut(s) 645
PspFI CCCAGC 1 cut(s) 983
PspGI CCWGG 4 cut(s) 781, 1697, 1932, 2141
PspN4I GGNNCC 4 cut(s) 1090, 1351, 1823, 2084
PspXI VCTCGAGB 1 cut(s) 466
PstI CTGCAG 2 cut(s) 1677, 2092
PstNI CAGNNNCTG 1 cut(s) 1960
PsuI RGATCY 1 cut(s) 1821
PvuII CAGCTG 2 cut(s) 1003, 1693
RsaI GTAC 7 cut(s) 10, 393, 490, 922, 1090, 1485, 1532
RsaNI GTAC 7 cut(s) 9, 392, 489, 921, 1089, 1484, 1531
RseI CAYNNNNRTG 4 cut(s) 1020, 1479, 1505, 2049
SaqAI TTAA 4 cut(s) 54, 665, 1046, 1230
SatI GCNGC 7 cut(s) 286, 1371, 1454, 1673, 1676, 2091, 2198
Sau3AI GATC 4 cut(s) 970, 1183, 1821, 2053
ScaI AGTACT 1 cut(s) 490
SchI GAGTC 7 cut(s) 41, 412, 602, 627, 713, 1531, 2274
ScrFI CCNGG 6 cut(s) 97, 783, 1699, 1934, 2117, 2143
SduI GDGCHC 2 cut(s) 1706, 2068
SexAI ACCWGGT 2 cut(s) 781, 1697
SfaNI GCATC 5 cut(s) 429, 1243, 1497, 1866, 2129
SfcI CTRYAG 2 cut(s) 1673, 2088
Sfr274I CTCGAG 2 cut(s) 466, 1705
SlaI CTCGAG 2 cut(s) 466, 1705
SmiMI CAYNNNNRTG 4 cut(s) 1020, 1479, 1505, 2049
SmlI CTYRAG 4 cut(s) 466, 1705, 1735, 1910
SmoI CTYRAG 4 cut(s) 466, 1705, 1735, 1910
SsiI CCGC 2 cut(s) 286, 1579
SspMI CTAG 6 cut(s) 72, 263, 507, 630, 678, 1211
StyD4I CCNGG 6 cut(s) 95, 781, 1697, 1932, 2115, 2141
StyI CCWWGG 1 cut(s) 506
TaaI ACNGT 9 cut(s) 13, 712, 765, 817, 920, 1217, 1922, 1940, 1962
TaqI TCGA 8 cut(s) 81, 143, 281, 467, 626, 973, 1706, 1809
TatI WGTACW 3 cut(s) 488, 920, 1483
TauI GCSGC 1 cut(s) 288
TfiI GAWTC 3 cut(s) 550, 1261, 1742
Tru1I TTAA 4 cut(s) 54, 665, 1046, 1230
Tru9I TTAA 4 cut(s) 54, 665, 1046, 1230
TscAI CASTG 7 cut(s) 295, 808, 915, 1615, 1823, 1927, 2143
TseFI GTSAC 2 cut(s) 91, 2179
TseI GCWGC 6 cut(s) 1370, 1453, 1672, 1675, 2090, 2197
Tsp45I GTSAC 2 cut(s) 91, 2179
TspGWI ACGGA 2 cut(s) 605, 2021
TspRI CASTG 7 cut(s) 295, 808, 915, 1615, 1823, 1927, 2143
Van91I CCANNNNNTGG 1 cut(s) 2142
VspI ATTAAT 1 cut(s) 1230
XapI RAATTY 6 cut(s) 269, 583, 693, 773, 797, 2234
XbaI TCTAGA 1 cut(s) 262
XceI RCATGY 3 cut(s) 1164, 1172, 1437
XcmI CCANNNNNNNNNTGG 2 cut(s) 145, 1387
XhoI CTCGAG 2 cut(s) 466, 1705
XmaJI CCTAGG 1 cut(s) 506
XmnI GAANNNNTTC 1 cut(s) 939
XspI CTAG 6 cut(s) 72, 263, 507, 630, 678, 1211
ZrmI AGTACT 1 cut(s) 490
Zsp2I ATGCAT 2 cut(s) 1154, 2050
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.