Rroxscaffold_1G00046010

Protein of unknown function (DUF2921)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000420
Physical Location & Seq
Forward (+)
65172855 .. 65175611
2757 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00046010.1

Sequence Viewer

Length: 2757 bp
ATGGCCATTTTCATGAAAATTTTCATTTTCTTCTTTGTTTTTGTCACACGTTCGTTAAACTCTGTTTCGTCCTCCACTTCAGATGCTCAAGTGTCTTTTGCTGATCATTGTGCTTCAATTGCTCCTCCTCACTACTCAACCCCACAAAGATATGCCGGACTTAATTCCTCTGTTCACCGCCTTACCGGTTTCTACTACAGTGCTGGTGAAACTAACATTCTTAGTCAGAACTCAATTGTATTCCGCTTCCGGAATGATGGAGCAGCGGATGAACAAGGCTTGTTCATGGTCCAAGGAAGTCTTCGATTTCCTAGTGACAGTACATATAACCTTGTGGGAAACTCTACAAGGAGCTGGCAATCCACCGACTTAAGAGTTCCATATGGACAGCGTCCCAGAAGCTTTAGGCTAAATGGGGTCTGGTCAGAATCTTCTGGGGAGCTTTGTATGGTTGGATCAGGTTTTAATTCCTCGGACCAAGGTAATTTACTGACCTTTCCTGTGGTTCTTAAACTACATAAACTCAAGAGTTCAACTAGTCTTACTAGTTTAATTAGTGGCACCTTAGAGAGCTTGGTAACTTCTAAGAATGAACCAAATTATTTTGGACCAATCTCTATATCATTGCTTCCTTCCATGAACTACCAGTACACTTTTGTTTCAAACAAATCTCATAACAGCTATTCGGATGGAAGTGTTGTTCCCCCAAGTTCTTTAAAAATAGGGAGATTTTGCAGGGTATTCTCAAGAGTAATACTATTCCGTGAGTTACAACTGAGATATTCAAGCCATTGTGGTTCTGCAAACAACTGCTCTCCTCTTGCTGTGTTTGATTTGCCTCGCGTCATGTCGTTGAATAATATTGAGTGTTTGGAGGATAGAAAAACTTTGCGACTTCTGGTAGATTTTGCAGCCAGTAGCATCAGTTACCAGAGGCCTTTCAATCCAACTACAACATTGGTTGCGGAAGGTTCATGGGATGCAAGGAAAAATCAGCTAAATGTTGTTGCTTGTCAGTTCGTGCGTGCAAGGAATTCTTTGAGTCCTAGTCATGTGGGTGATTGTTCAACAAGATTGACCATGGGATTTCCTTCAATATGGACAATTGGAAATTCTAGCTGCATTGTGGGGAAAATTTGGAGCAACAAATCCATGACAGAGGCAGGTTACTTCGAAAACATCACATTTGACAGTCCTCAAAGTGATTCAGCTGTATTTCAAGGCCAGAGGTATGAGTATACCAAAATAGACAAAGCAACAAAGTTGTGCCCAAGAAAGAAGACTGATGCTGATGACAAGAACAACATTTATCCAAATCCTTTTTCTTATTACATGGGATTGGATATTACAGCCAACAGTTCTACAGGAGAAGTTGGATGGGGCTATTCTGTCCCTCTCTCTGTTGATAACAAATTCTTCCAACCGTATTGGTACTCTCTGGAAGATGGAACTGAAGAATACCTTGGAGCTCCTGTGAGTGCTCCTGTGAGCGATAGAAACAGCAATAGCAACAGGTACAACATCAGCTACAGAATAAGCATTAAAGCAGTAGATGGGGTTAGGCTTCCCAGTGGAGTGTCCCAACTTAATTCATTATATGAGATGGGGATTTTTGCTGAAGGGATATATGATGGCAGAGATGGAAGCCTGTGTATGGTAGGTTGCCGATACTTGAGCTCAAACAGTCACCAACAGCCAACATATGATTCTGTAGACTGTGAGATTGTTGTCAATGTTCAGTTTCCTCCAACAAATCCAAACAGTACCTATTCGGGTTTTATCAAGGGAAGCATCGAAAGCACACGAGAAATGTCTGATCCTCTTTATTTTGAGGGATTGGATTTGACCTCAGCTGCTGGTAACATGGTCGAAGCAAGCCAATCCATTTGGAGAATGGATGTGGAGATCATATTGGTTGTTATATCCAACACACTCGCTTGTGTTTTTGGGGCATTACAACTCTTTCATGTGAAACGGAATCCGGATGTGCTCCCATCCATTTCTATCTTGATGCTGTATATTCTTGCTCTTGGCTACATGATACCTCTTATGCTAAACTTTGATGCCTTGTTCACACGACATGGTAGCCAAACTGTGTTCCTTGGAAGTGGATGGCTTGAACTCAATGAAGTAATTGTAAGGGTAGTAACAATGGTAGGTTTCTTGCTGCAAATTCGTCTTCTGCAGATAACAATGTCAGCAAAATCAGCAAATGGAAACCAAAAGGAGCTCTGGGCAGTGGAGAAGCTGGCTCTCTTTGTGGCTTTACCAGTATATGTTGGAGGGTCCTTGGTTGCTTTGCTTCTGATGAACTGGAGGAGGAACGATATTGCTGTGATGGTTTCGAGTGGTCAAAAACATCCCATTTTGGGTACTATTTTGAAATCTTGTGCTGGTTTGTTCTTGGATGCATTTTTGTTCCCTCAAATTCTTCTCAACATGTTCTCCAAATCAACGGAAAAGGCATTGTCAGTTTCGTTCTTCGTTGGAACAACTCTTGTTCGTGTGCTGCCACATGCATATGATCTTTACAGGGCTCATACCTCTAGTCACCAATTGAAAGATTCATACATTTATGCAAGCCCTGCAGCAGACTTTTACTCTACTGCTTGGAATGTCATTATTCCTTTTGGAGGTCTACTTTTTGCTGTGATAATCTACTTGCAGCAGCGCTTTGGGGGTTGCTGCATTCTTCCTCAGAAGTTCATAGAGTTGGGTGAATATGAGAAGGTGCCTATCGTTACTGAAGCGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
DUF2921

Protein Analysis

918

Amino Acids

101.88

Weight (kDa)

7.78

Isoelectric Point (pI)

43.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2921_N PF25333 62 - 206 2.9e-17 DUF2921 lipocalin-like domain
DUF2921_N PF25333 227 - 395 5.6e-27 DUF2921 lipocalin-like domain
DUF2921_N PF25333 421 - 613 4.4e-32 DUF2921 lipocalin-like domain
DUF2921 PF11145 625 - 902 1.6e-60 Transmembrane E3 ligase/DUF2921 transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000420)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21700
fragaria_vesca FvH4_1g10360 FvH4_1g10760 FvH4_2g30370 FvH4_3g09240 FvH4_3g20050 FvH4_3g20280 FvH4_3g20290 FvH4_3g20291 FvH4_3g20340 FvH4_3g20350 FvH4_3g20351 FvH4_3g20380 FvH4_3g20390 FvH4_5g13190 FvH4_6g20070 FvH4_6g42890
malus_domestica MD03G1227000.v1.1 MD10G1294900.v1.1 MD11G1246300.v1.1 MD11G1246400.v1.1 MD11G1246500.v1.1 MD11G1246600.v1.1
prunus_persica Prupe.4G047300_v2.0.a1 Prupe.4G181700_v2.0.a1 Prupe.4G181900_v2.0.a1 Prupe.4G182000_v2.0.a1 Prupe.4G182200_v2.0.a1
pyrus_communis pycom03g17470 pycom10g24670 pycom11g21640
rosa_chinensis RchiOBHm_Chr5g0008381 RchiOBHm_Chr5g0008391 RchiOBHm_Chr5g0033881 RchiOBHm_Chr5g0034171 RchiOBHm_Chr5g0034181 RchiOBHm_Chr5g0034191 RchiOBHm_Chr5g0034201 RchiOBHm_Chr5g0034211 RchiOBHm_Chr5g0034221 RchiOBHm_Chr5g0034231 RchiOBHm_Chr5g0034271 RchiOBHm_Chr5g0034281
rosa_laevigata RLG00000031566 RLG00000031567 RLG00000033506 RLG00000033529 RLG00000033530 RLG00000033531
rosa_multiflora Rmu_co8445509.1_g000001 Rmu_co8470615.1_g000001 Rmu_sc0008805.1_g000003 Rmu_sc0008805.1_g000004 Rmu_ssc0000128.1_g000029 Rmu_ssc0000128.1_g000031 Rmu_ssc0000128.1_g000032 Rmu_ssc0000388.1_g000062 Rmu_ssc0000388.1_g000063
rosa_roxburghii Rroxscaffold_1G00046010 Rroxscaffold_1G00046020 Rroxscaffold_1G00046030 Rroxscaffold_1G00046040 Rroxscaffold_1G00046060 Rroxscaffold_1G00046070 Rroxscaffold_1G00046080 Rroxscaffold_1G00046090
rosa_rugosa Rorug04G0435500 Rorug05G0142900 Rorug05G0144600 Rorug05G0144600 Rorug05G0144700 Rorug05G0144800
rosa_samantha Rh5AG233800 Rh5AG235900 Rh5AG236000 Rh5AG236100 Rh5AG236200 Rh5AG236300 Rh5AG236500 Rh5BG061200 Rh5BG061300 Rh5BG234300 Rh5BG236600 Rh5BG236800 Rh5CG071200 Rh5DG060000 Rh5DG060100 Rh5DG241300 Rh5DG243700 Rh5DG244000 Rh5DG244100
rosa_wichuraiana Rw3G018950 Rw5G005680 Rw5G021330 Rw5G021340 Rw5G021350 Rw5G021550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1154
AccB1I GGYRCC 2 cut(s) 560, 2731
AccI GTMKAC 3 cut(s) 1238, 1713, 2638
AccII CGCG 1 cut(s) 843
AccIII TCCGGA 2 cut(s) 249, 1981
AciI CCGC 4 cut(s) 178, 244, 266, 965
AclWI GGATC 2 cut(s) 463, 1811
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 7 cut(s) 18, 1033, 1111, 1134, 1412, 2172, 2427
AcuI CTGAAG 3 cut(s) 63, 1473, 1638
AfaI GTAC 6 cut(s) 322, 650, 1433, 1517, 1765, 2374
AfeI AGCGCT 1 cut(s) 2672
AfiI CCNNNNNNNGG 3 cut(s) 1654, 2369, 2633
AflII CTTAAG 1 cut(s) 370
AflIII ACRYGT 2 cut(s) 47, 2439
AgeI ACCGGT 1 cut(s) 185
AhlI ACTAGT 2 cut(s) 536, 545
AjuI GAANNNNNNNTTGG 8 cut(s) 285, 317, 1306, 1338, 1446, 1478, 1690, 1722
AloI GAACNNNNNNTCC 2 cut(s) 684, 716
Alw21I GWGCWC 5 cut(s) 1471, 1483, 1679, 1992, 2232
AlwI GGATC 2 cut(s) 463, 1811
AlwNI CAGNNNCTG 1 cut(s) 1856
Aor13HI TCCGGA 2 cut(s) 249, 1981
Aor51HI AGCGCT 1 cut(s) 2672
AoxI GGCC 3 cut(s) 3, 935, 1222
ApoI RAATTY 7 cut(s) 18, 1033, 1111, 1134, 1412, 2172, 2427
AsiGI ACCGGT 1 cut(s) 185
Asp700I GAANNNNTTC 1 cut(s) 20
AspLEI GCGC 1 cut(s) 2673
AspS9I GGNCC 4 cut(s) 289, 475, 608, 2286
AsuHPI GGTGA 6 cut(s) 167, 218, 1070, 1679, 2543, 2729
AsuII TTCGAA 1 cut(s) 1173
AvaII GGWCC 4 cut(s) 289, 475, 608, 2286
BaeGI GKGCMC 1 cut(s) 1271
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 560, 2731
BanII GRGCYC 4 cut(s) 1471, 1679, 2232, 2539
BauI CACGAG 1 cut(s) 1803
BbsI GAAGAC 3 cut(s) 293, 1286, 2171
Bbv12I GWGCWC 5 cut(s) 1471, 1483, 1679, 1992, 2232
BbvCI CCTCAGC 1 cut(s) 1849
BclI TGATCA 1 cut(s) 103
BcuI ACTAGT 2 cut(s) 536, 545
BfaI CTAG 6 cut(s) 312, 537, 546, 1047, 1116, 2547
BfmI CTRYAG 6 cut(s) 196, 1362, 1528, 1710, 2183, 2586
BfoI RGCGCY 1 cut(s) 2674
BfrI CTTAAG 1 cut(s) 370
BfuAI ACCTGC 1 cut(s) 1154
Bme18I GGWCC 4 cut(s) 289, 475, 608, 2286
BmgT120I GGNCC 4 cut(s) 289, 475, 608, 2286
BmiI GGNNCC 3 cut(s) 562, 2287, 2733
BmrI ACTGGG 1 cut(s) 1563
BmsI GCATC 8 cut(s) 73, 930, 970, 1276, 1800, 2001, 2053, 2398
BmuI ACTGGG 1 cut(s) 1563
BpiI GAAGAC 3 cut(s) 293, 1286, 2171
BpmI CTGGAG 1 cut(s) 2335
Bpu10I CCTNAGC 1 cut(s) 1849
Bpu14I TTCGAA 1 cut(s) 1173
BpuEI CTTGAG 4 cut(s) 72, 509, 730, 1693
BsaJI CCNNGG 7 cut(s) 292, 471, 478, 1080, 1462, 2101, 2289
BsaWI WCCGGW 3 cut(s) 185, 249, 1981
BsaXI ACNNNNNCTCC 4 cut(s) 1458, 1488, 2429, 2459
Bsc4I CCNNNNNNNGG 3 cut(s) 1654, 2369, 2633
Bse118I RCCGGY 1 cut(s) 185
Bse1I ACTGG 5 cut(s) 646, 915, 1569, 2270, 2318
Bse3DI GCAATG 1 cut(s) 623
BseAI TCCGGA 2 cut(s) 249, 1981
BseDI CCNNGG 7 cut(s) 292, 471, 478, 1080, 1462, 2101, 2289
BseLI CCNNNNNNNGG 3 cut(s) 1654, 2369, 2633
BseMI GCAATG 1 cut(s) 623
BseMII CTCAG 3 cut(s) 767, 1863, 2711
BseNI ACTGG 5 cut(s) 646, 915, 1569, 2270, 2318
BseRI GAGGAG 4 cut(s) 114, 117, 807, 2332
BseSI GKGCMC 1 cut(s) 1271
Bsh1236I CGCG 1 cut(s) 843
BshFI GGCC 3 cut(s) 5, 937, 1224
BshNI GGYRCC 2 cut(s) 560, 2731
BshTI ACCGGT 1 cut(s) 185
BsiHKAI GWGCWC 5 cut(s) 1471, 1483, 1679, 1992, 2232
BsiSI CCGG 4 cut(s) 156, 186, 250, 1982
BslFI GGGAC 3 cut(s) 378, 1376, 1564
BslI CCNNNNNNNGG 3 cut(s) 1654, 2369, 2633
BsmFI GGGAC 3 cut(s) 378, 1376, 1564
BsmI GAATGC 1 cut(s) 2688
BsnI GGCC 3 cut(s) 5, 937, 1224
Bsp119I TTCGAA 1 cut(s) 1173
Bsp1286I GDGCHC 7 cut(s) 1271, 1471, 1483, 1679, 1992, 2232, 2539
Bsp13I TCCGGA 2 cut(s) 249, 1981
Bsp143I GATC 5 cut(s) 103, 455, 1816, 1905, 2524
Bsp19I CCATGG 1 cut(s) 1080
BspACI CCGC 4 cut(s) 178, 244, 266, 965
BspANI GGCC 3 cut(s) 5, 937, 1224
BspCNI CTCAG 3 cut(s) 768, 1862, 2710
BspEI TCCGGA 2 cut(s) 249, 1981
BspFNI CGCG 1 cut(s) 843
BspHI TCATGA 1 cut(s) 12
BspLI GGNNCC 3 cut(s) 562, 2287, 2733
BspMAI CTGCAG 2 cut(s) 2187, 2590
BspMI ACCTGC 1 cut(s) 1154
BspPI GGATC 2 cut(s) 463, 1811
BspT104I TTCGAA 1 cut(s) 1173
BspT107I GGYRCC 2 cut(s) 560, 2731
BspTI CTTAAG 1 cut(s) 370
BsrDI GCAATG 1 cut(s) 623
BsrFI RCCGGY 1 cut(s) 185
BsrI ACTGG 5 cut(s) 646, 915, 1569, 2270, 2318
BssAI RCCGGY 1 cut(s) 185
BssECI CCNNGG 7 cut(s) 292, 471, 478, 1080, 1462, 2101, 2289
BssMI GATC 5 cut(s) 103, 455, 1816, 1905, 2524
BssNAI GTATAC 1 cut(s) 1239
BssSI CACGAG 1 cut(s) 1803
BssT1I CCWWGG 6 cut(s) 292, 478, 1080, 1462, 2101, 2289
Bst1107I GTATAC 1 cut(s) 1239
Bst2BI CACGAG 1 cut(s) 1803
Bst4CI ACNGT 9 cut(s) 200, 320, 1193, 1358, 1425, 1685, 1718, 1763, 2095
BstAFI CTTAAG 1 cut(s) 370
BstAPI GCANNNNNTGC 1 cut(s) 2585
BstBI TTCGAA 1 cut(s) 1173
BstC8I GCNNGC 5 cut(s) 356, 1026, 1876, 2250, 2581
BstDEI CTNAG 6 cut(s) 221, 565, 585, 776, 1849, 2697
BstDSI CCRYGG 1 cut(s) 1080
BstENI CCTNNNNNAGG 1 cut(s) 2631
BstFNI CGCG 1 cut(s) 843
BstH2I RGCGCY 1 cut(s) 2674
BstHHI GCGC 1 cut(s) 2673
BstKTI GATC 5 cut(s) 106, 458, 1819, 1908, 2527
BstMBI GATC 5 cut(s) 103, 455, 1816, 1905, 2524
BstMWI GCNNNNNNNGC 4 cut(s) 119, 1797, 2207, 2585
BstNSI RCATGY 2 cut(s) 2443, 2519
BstSFI CTRYAG 6 cut(s) 196, 1362, 1528, 1710, 2183, 2586
BstSLI GKGCMC 1 cut(s) 1271
BstUI CGCG 1 cut(s) 843
BstV2I GAAGAC 3 cut(s) 293, 1286, 2171
BstZ17I GTATAC 1 cut(s) 1239
BsuRI GGCC 3 cut(s) 5, 937, 1224
BtgI CCRYGG 1 cut(s) 1080
BtsI GCAGTG 1 cut(s) 2244
BtsIMutI CAGTG 3 cut(s) 205, 1576, 2244
BveI ACCTGC 1 cut(s) 1154
Cac8I GCNNGC 5 cut(s) 356, 1026, 1876, 2250, 2581
CaiI CAGNNNCTG 1 cut(s) 1856
CciI TCATGA 1 cut(s) 12
CfoI GCGC 1 cut(s) 2673
Cfr10I RCCGGY 1 cut(s) 185
Cfr13I GGNCC 4 cut(s) 289, 475, 608, 2286
CseI GACGC 3 cut(s) 380, 832, 2739
Csp6I GTAC 6 cut(s) 321, 649, 1432, 1516, 1764, 2373
CspAI ACCGGT 1 cut(s) 185
CviQI GTAC 6 cut(s) 321, 649, 1432, 1516, 1764, 2373
DdeI CTNAG 6 cut(s) 221, 565, 585, 776, 1849, 2697
DpnI GATC 5 cut(s) 105, 457, 1818, 1907, 2526
DpnII GATC 5 cut(s) 103, 455, 1816, 1905, 2524
DraI TTTAAA 1 cut(s) 717
EaeI YGGCCR 1 cut(s) 3
Ecl136II GAGCTC 3 cut(s) 1469, 1677, 2230
Eco130I CCWWGG 6 cut(s) 292, 478, 1080, 1462, 2101, 2289
Eco147I AGGCCT 1 cut(s) 937
Eco24I GRGCYC 4 cut(s) 1471, 1679, 2232, 2539
Eco47I GGWCC 4 cut(s) 289, 475, 608, 2286
Eco47III AGCGCT 1 cut(s) 2672
Eco53kI GAGCTC 3 cut(s) 1469, 1677, 2230
Eco57I CTGAAG 3 cut(s) 63, 1473, 1638
EcoICRI GAGCTC 3 cut(s) 1469, 1677, 2230
EcoNI CCTNNNNNAGG 1 cut(s) 2631
EcoO109I RGGNCCY 1 cut(s) 2286
EcoRI GAATTC 1 cut(s) 1033
EcoT14I CCWWGG 6 cut(s) 292, 478, 1080, 1462, 2101, 2289
EcoT22I ATGCAT 2 cut(s) 2413, 2521
EcoT38I GRGCYC 4 cut(s) 1471, 1679, 2232, 2539
ErhI CCWWGG 6 cut(s) 292, 478, 1080, 1462, 2101, 2289
FalI AAGNNNNNCTT 6 cut(s) 285, 317, 1021, 1053, 1446, 1478
FaqI GGGAC 3 cut(s) 378, 1376, 1564
FauNDI CATATG 3 cut(s) 382, 1702, 2521
FbaI TGATCA 1 cut(s) 103
FblI GTMKAC 3 cut(s) 1238, 1713, 2638
FriOI GRGCYC 4 cut(s) 1471, 1679, 2232, 2539
FspBI CTAG 6 cut(s) 312, 537, 546, 1047, 1116, 2547
GlaI GCGC 1 cut(s) 2672
GsuI CTGGAG 1 cut(s) 2335
HaeII RGCGCY 1 cut(s) 2674
HaeIII GGCC 3 cut(s) 5, 937, 1224
HapII CCGG 4 cut(s) 156, 186, 250, 1982
HgaI GACGC 3 cut(s) 380, 832, 2739
HhaI GCGC 1 cut(s) 2673
Hin6I GCGC 1 cut(s) 2671
HinP1I GCGC 1 cut(s) 2671
HindIII AAGCTT 1 cut(s) 400
HinfI GANTC 6 cut(s) 428, 1042, 1205, 1706, 1978, 2564
HpaII CCGG 4 cut(s) 156, 186, 250, 1982
HphI GGTGA 6 cut(s) 167, 218, 1070, 1679, 2543, 2729
Hpy166II GTNNAC 6 cut(s) 175, 651, 1239, 1714, 2073, 2639
Hpy188I TCNGA 8 cut(s) 82, 228, 427, 475, 688, 1816, 2307, 2700
Hpy188III TCNNGA 7 cut(s) 13, 250, 526, 747, 1439, 1982, 2008
Hpy8I GTNNAC 6 cut(s) 175, 651, 1239, 1714, 2073, 2639
HpyAV CCTTC 5 cut(s) 642, 962, 1101, 1613, 2722
HpyCH4III ACNGT 9 cut(s) 200, 320, 1193, 1358, 1425, 1685, 1718, 1763, 2095
HpyCH4IV ACGT 1 cut(s) 49
HpyF10VI GCNNNNNNNGC 4 cut(s) 119, 1797, 2207, 2585
HpyF3I CTNAG 6 cut(s) 221, 565, 585, 776, 1849, 2697
HpySE526I ACGT 1 cut(s) 49
HspAI GCGC 1 cut(s) 2671
Kpn2I TCCGGA 2 cut(s) 249, 1981
Ksp22I TGATCA 1 cut(s) 103
Kzo9I GATC 5 cut(s) 103, 455, 1816, 1905, 2524
LweI GCATC 8 cut(s) 73, 930, 970, 1276, 1800, 2001, 2053, 2398
MaeI CTAG 6 cut(s) 312, 537, 546, 1047, 1116, 2547
MaeII ACGT 1 cut(s) 49
MalI GATC 5 cut(s) 105, 457, 1818, 1907, 2526
MboI GATC 5 cut(s) 103, 455, 1816, 1905, 2524
MfeI CAATTG 4 cut(s) 117, 234, 1104, 2555
MhlI GDGCHC 7 cut(s) 1271, 1471, 1483, 1679, 1992, 2232, 2539
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 1051
MmeI TCCRAC 8 cut(s) 433, 971, 1354, 1444, 1772, 1950, 2260, 2467
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 2 cut(s) 2413, 2521
MroI TCCGGA 2 cut(s) 249, 1981
MroXI GAANNNNTTC 1 cut(s) 20
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 3 cut(s) 11, 1056, 2520
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 3 cut(s) 266, 1211, 1853
MspCI CTTAAG 1 cut(s) 370
MspI CCGG 4 cut(s) 156, 186, 250, 1982
MunI CAATTG 4 cut(s) 117, 234, 1104, 2555
Mva1269I GAATGC 1 cut(s) 2688
MvnI CGCG 1 cut(s) 843
MwoI GCNNNNNNNGC 4 cut(s) 119, 1797, 2207, 2585
NcoI CCATGG 1 cut(s) 1080
NdeI CATATG 3 cut(s) 382, 1702, 2521
NdeII GATC 5 cut(s) 103, 455, 1816, 1905, 2524
NlaIV GGNNCC 3 cut(s) 562, 2287, 2733
NmuCI GTSAC 4 cut(s) 43, 314, 1685, 2549
NsiI ATGCAT 2 cut(s) 2413, 2521
NspI RCATGY 2 cut(s) 2443, 2519
NspV TTCGAA 1 cut(s) 1173
PagI TCATGA 1 cut(s) 12
PceI AGGCCT 1 cut(s) 937
PciI ACATGT 1 cut(s) 2439
PctI GAATGC 1 cut(s) 2688
PdmI GAANNNNTTC 1 cut(s) 20
PfeI GAWTC 5 cut(s) 428, 1205, 1706, 1978, 2564
PflFI GACNNNGTC 1 cut(s) 390
PinAI ACCGGT 1 cut(s) 185
PleI GAGTC 1 cut(s) 1050
PpsI GAGTC 1 cut(s) 1050
PpuMI RGGWCCY 1 cut(s) 2286
PscI ACATGT 1 cut(s) 2439
Psp124BI GAGCTC 3 cut(s) 1471, 1679, 2232
Psp5II RGGWCCY 1 cut(s) 2286
PspN4I GGNNCC 3 cut(s) 562, 2287, 2733
PspPI GGNCC 4 cut(s) 289, 475, 608, 2286
PspPPI RGGWCCY 1 cut(s) 2286
PsrI GAACNNNNNNTAC 2 cut(s) 632, 664
PstI CTGCAG 2 cut(s) 2187, 2590
PstNI CAGNNNCTG 1 cut(s) 1856
PsyI GACNNNGTC 1 cut(s) 390
PvuII CAGCTG 2 cut(s) 1211, 1853
RsaI GTAC 6 cut(s) 322, 650, 1433, 1517, 1765, 2374
RsaNI GTAC 6 cut(s) 321, 649, 1432, 1516, 1764, 2373
RseI CAYNNNNRTG 3 cut(s) 11, 1056, 2520
SacI GAGCTC 3 cut(s) 1471, 1679, 2232
Sau3AI GATC 5 cut(s) 103, 455, 1816, 1905, 2524
Sau96I GGNCC 4 cut(s) 289, 475, 608, 2286
SchI GAGTC 1 cut(s) 1051
SduI GDGCHC 7 cut(s) 1271, 1471, 1483, 1679, 1992, 2232, 2539
SfaNI GCATC 8 cut(s) 73, 930, 970, 1276, 1800, 2001, 2053, 2398
SfcI CTRYAG 6 cut(s) 196, 1362, 1528, 1710, 2183, 2586
SfuI TTCGAA 1 cut(s) 1173
SinI GGWCC 4 cut(s) 289, 475, 608, 2286
SmiMI CAYNNNNRTG 3 cut(s) 11, 1056, 2520
SmlI CTYRAG 5 cut(s) 87, 370, 524, 745, 1672
SmoI CTYRAG 5 cut(s) 87, 370, 524, 745, 1672
SpeI ACTAGT 2 cut(s) 536, 545
SseBI AGGCCT 1 cut(s) 937
SsiI CCGC 4 cut(s) 178, 244, 266, 965
SspI AATATT 1 cut(s) 862
SspMI CTAG 6 cut(s) 312, 537, 546, 1047, 1116, 2547
SstI GAGCTC 3 cut(s) 1471, 1679, 2232
StuI AGGCCT 1 cut(s) 937
StyI CCWWGG 6 cut(s) 292, 478, 1080, 1462, 2101, 2289
TaaI ACNGT 9 cut(s) 200, 320, 1193, 1358, 1425, 1685, 1718, 1763, 2095
TaiI ACGT 1 cut(s) 52
TaqI TCGA 5 cut(s) 304, 1173, 1794, 1869, 2345
TatI WGTACW 2 cut(s) 320, 648
TfiI GAWTC 5 cut(s) 428, 1205, 1706, 1978, 2564
TscAI CASTG 3 cut(s) 205, 1576, 2244
TseFI GTSAC 4 cut(s) 43, 314, 1685, 2549
Tsp45I GTSAC 4 cut(s) 43, 314, 1685, 2549
TspGWI ACGGA 3 cut(s) 752, 1990, 2471
TspRI CASTG 3 cut(s) 205, 1576, 2244
Tth111I GACNNNGTC 1 cut(s) 390
Vha464I CTTAAG 1 cut(s) 370
VpaK11BI GGWCC 4 cut(s) 289, 475, 608, 2286
XagI CCTNNNNNAGG 1 cut(s) 2631
XapI RAATTY 7 cut(s) 18, 1033, 1111, 1134, 1412, 2172, 2427
XceI RCATGY 2 cut(s) 2443, 2519
XcmI CCANNNNNNNNNTGG 1 cut(s) 1891
XmiI GTMKAC 3 cut(s) 1238, 1713, 2638
XmnI GAANNNNTTC 1 cut(s) 20
XspI CTAG 6 cut(s) 312, 537, 546, 1047, 1116, 2547
Zsp2I ATGCAT 2 cut(s) 2413, 2521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.