FvH4_3g11693

VWA domain containing CoxE-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000360
Physical Location & Seq
Forward (+)
6906171 .. 6909572
3402 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g11693.t1

Sequence Viewer

Length: 2025 bp
ATGTCTTCTCAGTCTGCATCTGATCAAAACAACTGTGGCATCTGTCTGAGTAGCATGGCAGCCCCAAAAGGCCAAGCCATATTCACTGCTCAATGCTCCCACTCCTTCCACTATCCTTGCATTGTCCAGAATGTTCAGCATGGAAATCTTTGTTGCCCCATTTGCCGAGTAATATGGGATAAGCAAAATGTTCCTTTCCAACCAAACTGGACTAGTACTTTTCCCCCTCAACATAACAACTCAGGTGATCCCCCTCACCCATGGGGTGTTAATTTCCCTCAACATAATAACTTGGGTGGGAACTCTCCCAACATCTCATTCTCATTCCAGCCGCGTCAGTCACCCCATCAGACTTATCATCAGTACTCATATCAGCAACCCAGTTGGAACTATCCTTCCTTCCCATTCATGCAGCAACACCAAGCCTTTCCCGAGCCATCTAGCTTCTCTGATGATGAACCTCTTTTGTCCACCTCACCAATGCAATCCAATGACCCCCAGGCTGTAACAATTAAGATTCAGACCGAGTCCTTGGCTATCTCAGCTGCAGAATCTCATCCGCGATATCCTGTTCTTGTCAGCATTTGTGCACCATCTCTTCAAGATACTGATGGCCAGGGGCGTACCCCCATCGACCTTGTGACAGTTTTGGATATAAGTGGCAGCATGTGTGGCCTAAAGCTTGACCTTGTGAAGCGCGCTGTCAAATTTATCATACAAAACTTGGGGCCTTCAGACCGACTTTCAATAGTTACATTTTCAACTACTGCTAGAAGAGTCCTTCCTCTAAGAAGAATGTCTGTTGAAGGCCGTGAAAATGCTGTGCGAGCTGTCAATTCTCTTAGACCAGATAACATGACCAACATTGTGGCAGGACTCGAGATAGGAACTCGGGTCCTTGAAGAACGAAGGGAGAGGAACCCAGTTGCTAGCATCATGCTTTTATCCGATGGCATGGATACTTACTGTCATAGTTCAAGCCAACTCTTGGCCCAATTGCCTGATTCAATTCGCTCTAATTGCATGCAACATGAAATTCCAGTTCACACATTTGGGTTTGGCAGCGACCATGATCCAAATACTATGCATGCTATATCTAATGCATCAGGTGGCACCTTTTCGTTTATCGAATCAGTTGGCATGATTCAAGATTCATTTGCTCTGTGCATTGGTGGTCTGCTCAGTGTTGTGGCTCAGGAAGCTCGCCTAACAGTAAGGTCAGCATCACATGGTGTGAAGATAGTAGCTATTCCATCAGGGAGATATATCAAAACTATATCTGATGAGGACCTGCAAGGTGTTGTTGATGTTGGGAGTCTCTATGCAGAAGAGGAGAAACAATTTCTGGTGTACCTCTTTGTTCCACAATCCTCAACTTCAGATACTAAAACTTCATTGTTAGATGTGTCATGCGTGTACAGGGATCTAGCTTCAAATGAGTTAACCCAGGTGCAAGGTGAGCTAGTTGAGATATTGAGACCTGAGGTTTGTTCCCCTACACATCAAGTAGTCTCTTTGGAGGTTGATCGCCAGCGCAATAGAATCTTGGTCTCTGAAACTATAGCAGAGGCACAAGGGTTGGCAGAGAATGGAAATCTGGAGGCTGCACGGGCCATTTTGACTAAACGAAGAGAATCTCTCTTAACAACACCAGCAGCCCGAGCTGGGGATGGTCTTAGCAATTTGCTTGAAACTGAGCTTCAAGAAGTCATGGATAGAATGGCAACCATGGATTTGTATACACACACCGGGCGCGCTTATGCGCTCTCGGGAATGAGCTCTCATTCACTGCAGAGAGCCACAACTAGGGGTGACACAATTACTTCATGCCTCTCTGCAAGTTTTGGTGCTGCTCAGGCTTCACACTCCACTGTGGGTACCTTTCAGCAGCAGCAGTGTGTCTCTGCAGGTGCCACTCCATTTGGGAGTCCAGCATCATTGCCACGAGGTGGTGCTTATGAAACATCTTCAATGGTTAGAATGGTACAGAAATCAAAGGATCTTGGCACTAGTTATACTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VWA Vwaint zf-RING_11 zf-RING_2

Protein Analysis

675

Amino Acids

73.46

Weight (kDa)

5.92

Isoelectric Point (pI)

49.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 10 - 56 2e-06 Ring finger domain
zf-RING_11 PF17123 11 - 41 1.5e-09 RING-like zinc finger
zf-C3HC4 PF00097 12 - 55 3.9e-06 Zinc finger, C3HC4 type (RING finger)
VWA PF00092 212 - 378 6.1e-20 von Willebrand factor type A domain
VWA_3 PF13768 212 - 375 8.7e-18 von Willebrand factor type A domain
VWA_2 PF13519 213 - 318 1.1e-15 von Willebrand factor type A domain
Vwaint PF14624 560 - 666 1.6e-11 VWA / Hh protein intein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000360)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11691 FvH4_3g11693 FvH4_3g11740 FvH4_3g11750 FvH4_3g11770 FvH4_3g11771 FvH4_3g11772 FvH4_3g11780
malus_domestica MD10G1239000.v1.1
prunus_persica Prupe.4G105400_v2.0.a1
pyrus_communis pycom10g20000
rosa_chinensis RchiOBHm_Chr5g0018981 RchiOBHm_Chr5g0018991 RchiOBHm_Chr5g0019101 RchiOBHm_Chr5g0019111 RchiOBHm_Chr5g0019121 RchiOBHm_Chr5g0019151 RchiOBHm_Chr5g0019161 RchiOBHm_Chr5g0019171 RchiOBHm_Chr5g0019181 RchiOBHm_Chr5g0019201
rosa_laevigata RLG00000032411 RLG00000032418 RLG00000032419 RLG00000032420 RLG00000032421 RLG00000032423 RLG00000032424 RLG00000032426
rosa_multiflora Rmu_co8475613.1_g000001 Rmu_co8503587.1_g000001 Rmu_sc0000563.1_g000023 Rmu_sc0000563.1_g000027 Rmu_sc0002142.1_g000001 Rmu_sc0002142.1_g000003 Rmu_sc0002142.1_g000004 Rmu_sc0002142.1_g000011 Rmu_sc0002142.1_g000022 Rmu_sc0002561.1_g000002 Rmu_sc0042836.1_g000001
rosa_roxburghii Rroxscaffold_1G00058790 Rroxscaffold_1G00058810 Rroxscaffold_1G00058820 Rroxscaffold_1G00058840 Rroxscaffold_1G00058850 Rroxscaffold_1G00058860 Rroxscaffold_1G00058880 Rroxscaffold_1G00058910 Rroxscaffold_1G00074090 Rroxscaffold_1G00074110 Rroxscaffold_1G00074120 Rroxscaffold_1G00074130 Rroxscaffold_1G00074150
rosa_rugosa Rorug05G0046800 Rorug05G0047200 Rorug05G0047300 Rorug05G0047400 Rorug05G0047600 Rorug05G0047600 Rorug05G0047800 Rorug05G0047900 Rorug05G0048000 Rorug05G0048100 Rorug05G0048100 Rorug05G0048200 Rorug05G0048300
rosa_samantha Rh4DG102800 Rh5AG138700 Rh5AG138900 Rh5AG139000 Rh5AG139100 Rh5AG139200 Rh5AG139400 Rh5AG139500 Rh5AG139600 Rh5BG137400 Rh5BG137600 Rh5BG137800 Rh5BG138100 Rh5BG138200 Rh5BG138300 Rh5BG138500 Rh5CG148800 Rh5CG148900 Rh5CG149200 Rh5CG149300 Rh5CG149400 Rh5CG149600 Rh5CG149700 Rh5CG150000 Rh5DG138200 Rh5DG138300 Rh5DG138500 Rh5DG138600 Rh5DG138700 Rh5DG138900 Rh5DG139000 Rh5DG139200
rosa_wichuraiana Rw5G012290 Rw5G012330 Rw5G012340 Rw5G012350 Rw5G012370 Rw5G012380 Rw5G012390 Rw5G012410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1898
Acc36I ACCTGC 2 cut(s) 1299, 1898
Acc65I GGTACC 1 cut(s) 1877
AccB1I GGYRCC 3 cut(s) 1112, 1877, 1910
AccB7I CCANNNNNTGG 2 cut(s) 988, 1949
AccI GTMKAC 1 cut(s) 1739
AccII CGCG 4 cut(s) 334, 562, 699, 1755
AciI CCGC 2 cut(s) 332, 560
AclWI GGATC 4 cut(s) 242, 1067, 1431, 2007
AcoI YGGCCR 1 cut(s) 613
AcsI RAATTY 2 cut(s) 707, 1035
AcuI CTGAAG 2 cut(s) 717, 1362
AdeI CACNNNGTG 2 cut(s) 1232, 1949
AfaI GTAC 7 cut(s) 217, 365, 625, 1352, 1418, 1879, 1986
AfiI CCNNNNNNNGG 5 cut(s) 988, 1665, 1666, 1806, 1949
AhlI ACTAGT 2 cut(s) 212, 2009
AjnI CCWGG 3 cut(s) 498, 615, 1446
AjuI GAANNNNNNNTTGG 2 cut(s) 302, 334
AloI GAACNNNNNNTCC 2 cut(s) 380, 412
Alw21I GWGCWC 2 cut(s) 592, 1781
Alw26I GTCTC 5 cut(s) 1322, 1471, 1516, 1555, 1906
Alw44I GTGCAC 1 cut(s) 588
AlwI GGATC 4 cut(s) 242, 1067, 1431, 2007
Ama87I CYCGRG 5 cut(s) 431, 878, 891, 1659, 1768
AoxI GGCC 7 cut(s) 70, 613, 673, 728, 808, 990, 1611
ApaLI GTGCAC 1 cut(s) 588
ApoI RAATTY 2 cut(s) 707, 1035
ArsI GACNNNNNNTTYG 2 cut(s) 1470, 1502
Asp718I GGTACC 1 cut(s) 1877
AspLEI GCGC 6 cut(s) 699, 701, 1536, 1755, 1757, 1765
AspS9I GGNCC 5 cut(s) 728, 895, 991, 1288, 1611
AsuC2I CCSGG 1 cut(s) 1750
AsuHPI GGTGA 6 cut(s) 248, 257, 333, 468, 1469, 1823
AsuNHI GCTAGC 1 cut(s) 929
AvaI CYCGRG 5 cut(s) 431, 878, 891, 1659, 1768
AvaII GGWCC 2 cut(s) 895, 1288
AxyI CCTNAGG 1 cut(s) 1482
BaeGI GKGCMC 1 cut(s) 592
BalI TGGCCA 1 cut(s) 615
BanI GGYRCC 3 cut(s) 1112, 1877, 1910
BanII GRGCYC 1 cut(s) 1781
BauI CACGAG 1 cut(s) 1944
Bbv12I GWGCWC 2 cut(s) 592, 1781
BccI CCATC 8 cut(s) 354, 445, 601, 605, 638, 944, 1261, 1664
BceAI ACGGC 1 cut(s) 795
BciT130I CCWGG 3 cut(s) 500, 617, 1448
BciVI GTATCC 1 cut(s) 952
BclI TGATCA 1 cut(s) 22
BcnI CCSGG 1 cut(s) 1750
BcoDI GTCTC 5 cut(s) 1322, 1471, 1516, 1555, 1906
BcuI ACTAGT 2 cut(s) 212, 2009
BfaI CTAG 8 cut(s) 213, 441, 771, 930, 1427, 1463, 1806, 2010
BfmI CTRYAG 4 cut(s) 546, 1560, 1790, 1905
BfuAI ACCTGC 2 cut(s) 1299, 1898
BfuI GTATCC 1 cut(s) 952
BmcAI AGTACT 2 cut(s) 217, 365
Bme1390I CCNGG 4 cut(s) 500, 617, 1448, 1750
Bme18I GGWCC 2 cut(s) 895, 1288
BmeT110I CYCGRG 5 cut(s) 431, 878, 891, 1659, 1768
BmgT120I GGNCC 5 cut(s) 728, 895, 991, 1288, 1611
BmiI GGNNCC 6 cut(s) 729, 896, 920, 1114, 1879, 1912
BmrFI CCNGG 4 cut(s) 500, 617, 1448, 1750
BmrI ACTGGG 2 cut(s) 375, 917
BmsI GCATC 6 cut(s) 26, 48, 942, 1112, 1232, 1943
BmtI GCTAGC 1 cut(s) 933
BmuI ACTGGG 2 cut(s) 375, 917
BplI GAGNNNNNCTC 2 cut(s) 1623, 1655
BpmI CTGGAG 1 cut(s) 1619
Bpu10I CCTNAGC 2 cut(s) 1194, 1854
BpuMI CCSGG 1 cut(s) 1750
BsaI GGTCTC 2 cut(s) 1471, 1555
BsaJI CCNNGG 6 cut(s) 260, 498, 531, 616, 1446, 1728
Bsc4I CCNNNNNNNGG 5 cut(s) 988, 1665, 1666, 1806, 1949
Bse1I ACTGG 4 cut(s) 212, 381, 923, 1040
Bse21I CCTNAGG 1 cut(s) 1482
Bse3DI GCAATG 1 cut(s) 1937
BseBI CCWGG 3 cut(s) 500, 617, 1448
BseDI CCNNGG 6 cut(s) 260, 498, 531, 616, 1446, 1728
BseGI GGATG 2 cut(s) 556, 1675
BseLI CCNNNNNNNGG 5 cut(s) 988, 1665, 1666, 1806, 1949
BseMI GCAATG 1 cut(s) 1937
BseMII CTCAG 9 cut(s) 23, 38, 255, 555, 1195, 1208, 1473, 1686, 1868
BseNI ACTGG 4 cut(s) 212, 381, 923, 1040
BsePI GCGCGC 2 cut(s) 697, 1753
BseRI GAGGAG 1 cut(s) 1346
BseSI GKGCMC 1 cut(s) 592
BseYI CCCAGC 1 cut(s) 1664
BsgI GTGCAG 1 cut(s) 1590
Bsh1236I CGCG 4 cut(s) 334, 562, 699, 1755
BshFI GGCC 7 cut(s) 72, 615, 675, 730, 810, 992, 1613
BshNI GGYRCC 3 cut(s) 1112, 1877, 1910
BsiHKAI GWGCWC 2 cut(s) 592, 1781
BsiHKCI CYCGRG 5 cut(s) 431, 878, 891, 1659, 1768
BsiSI CCGG 1 cut(s) 1749
BslI CCNNNNNNNGG 5 cut(s) 988, 1665, 1666, 1806, 1949
BsmAI GTCTC 5 cut(s) 1322, 1471, 1516, 1555, 1906
BsnI GGCC 7 cut(s) 72, 615, 675, 730, 810, 992, 1613
Bso31I GGTCTC 2 cut(s) 1471, 1555
BsoBI CYCGRG 5 cut(s) 431, 878, 891, 1659, 1768
Bsp1286I GDGCHC 2 cut(s) 592, 1781
Bsp1407I TGTACA 1 cut(s) 1416
Bsp143I GATC 6 cut(s) 22, 247, 1072, 1423, 1525, 1999
Bsp19I CCATGG 2 cut(s) 260, 1728
BspACI CCGC 2 cut(s) 332, 560
BspANI GGCC 7 cut(s) 72, 615, 675, 730, 810, 992, 1613
BspCNI CTCAG 9 cut(s) 22, 39, 254, 554, 1194, 1207, 1474, 1687, 1867
BspFNI CGCG 4 cut(s) 334, 562, 699, 1755
BspLI GGNNCC 6 cut(s) 729, 896, 920, 1114, 1879, 1912
BspMAI CTGCAG 3 cut(s) 550, 1794, 1909
BspMI ACCTGC 2 cut(s) 1299, 1898
BspOI GCTAGC 1 cut(s) 933
BspPI GGATC 4 cut(s) 242, 1067, 1431, 2007
BspT107I GGYRCC 3 cut(s) 1112, 1877, 1910
BspTNI GGTCTC 2 cut(s) 1471, 1555
BsrDI GCAATG 1 cut(s) 1937
BsrGI TGTACA 1 cut(s) 1416
BsrI ACTGG 4 cut(s) 212, 381, 923, 1040
BssECI CCNNGG 6 cut(s) 260, 498, 531, 616, 1446, 1728
BssHII GCGCGC 2 cut(s) 697, 1753
BssMI GATC 6 cut(s) 22, 247, 1072, 1423, 1525, 1999
BssNAI GTATAC 1 cut(s) 1740
BssSI CACGAG 1 cut(s) 1944
BssT1I CCWWGG 3 cut(s) 260, 531, 1728
Bst1107I GTATAC 1 cut(s) 1740
Bst2BI CACGAG 1 cut(s) 1944
Bst2UI CCWGG 3 cut(s) 500, 617, 1448
Bst4CI ACNGT 5 cut(s) 35, 646, 968, 1213, 1873
Bst6I CTCTTC 4 cut(s) 603, 769, 1323, 1624
BstAUI TGTACA 1 cut(s) 1416
BstC8I GCNNGC 8 cut(s) 699, 828, 931, 1025, 1089, 1204, 1532, 1755
BstDSI CCRYGG 2 cut(s) 260, 1728
BstF5I GGATG 2 cut(s) 556, 1675
BstFNI CGCG 4 cut(s) 334, 562, 699, 1755
BstHHI GCGC 6 cut(s) 699, 701, 1536, 1755, 1757, 1765
BstKTI GATC 6 cut(s) 25, 250, 1075, 1426, 1528, 2002
BstMAI GTCTC 5 cut(s) 1322, 1471, 1516, 1555, 1906
BstMBI GATC 6 cut(s) 22, 247, 1072, 1423, 1525, 1999
BstNI CCWGG 3 cut(s) 500, 617, 1448
BstNSI RCATGY 3 cut(s) 670, 1027, 1091
BstSCI CCNGG 4 cut(s) 498, 615, 1446, 1748
BstSFI CTRYAG 4 cut(s) 546, 1560, 1790, 1905
BstSLI GKGCMC 1 cut(s) 592
BstUI CGCG 4 cut(s) 334, 562, 699, 1755
BstX2I RGATCY 2 cut(s) 1423, 1999
BstXI CCANNNNNNTGG 1 cut(s) 868
BstYI RGATCY 2 cut(s) 1423, 1999
BstZ17I GTATAC 1 cut(s) 1740
Bsu36I CCTNAGG 1 cut(s) 1482
BsuI GTATCC 1 cut(s) 952
BsuRI GGCC 7 cut(s) 72, 615, 675, 730, 810, 992, 1613
BtgI CCRYGG 2 cut(s) 260, 1728
BtsCI GGATG 2 cut(s) 556, 1675
BtsI GCAGTG 3 cut(s) 84, 1787, 1901
BtsIMutI CAGTG 5 cut(s) 84, 1189, 1787, 1869, 1901
BveI ACCTGC 2 cut(s) 1299, 1898
Cac8I GCNNGC 8 cut(s) 699, 828, 931, 1025, 1089, 1204, 1532, 1755
CfoI GCGC 6 cut(s) 699, 701, 1536, 1755, 1757, 1765
Cfr13I GGNCC 5 cut(s) 728, 895, 991, 1288, 1611
CseI GACGC 1 cut(s) 323
Csp6I GTAC 7 cut(s) 216, 364, 624, 1351, 1417, 1878, 1985
CspCI CAANNNNNGTGG 4 cut(s) 98, 133, 1903, 1938
CviQI GTAC 7 cut(s) 216, 364, 624, 1351, 1417, 1878, 1985
DpnI GATC 6 cut(s) 24, 249, 1074, 1425, 1527, 2001
DpnII GATC 6 cut(s) 22, 247, 1072, 1423, 1525, 1999
DraIII CACNNNGTG 2 cut(s) 1232, 1949
EaeI YGGCCR 1 cut(s) 613
Eam1104I CTCTTC 4 cut(s) 603, 769, 1323, 1624
EarI CTCTTC 4 cut(s) 603, 769, 1323, 1624
Ecl136II GAGCTC 1 cut(s) 1779
Eco130I CCWWGG 3 cut(s) 260, 531, 1728
Eco24I GRGCYC 1 cut(s) 1781
Eco31I GGTCTC 2 cut(s) 1471, 1555
Eco32I GATATC 1 cut(s) 566
Eco47I GGWCC 2 cut(s) 895, 1288
Eco53kI GAGCTC 1 cut(s) 1779
Eco57I CTGAAG 2 cut(s) 717, 1362
Eco81I CCTNAGG 1 cut(s) 1482
Eco88I CYCGRG 5 cut(s) 431, 878, 891, 1659, 1768
EcoICRI GAGCTC 1 cut(s) 1779
EcoO109I RGGNCCY 3 cut(s) 728, 895, 1288
EcoRII CCWGG 3 cut(s) 498, 615, 1446
EcoRV GATATC 1 cut(s) 566
EcoT14I CCWWGG 3 cut(s) 260, 531, 1728
EcoT22I ATGCAT 2 cut(s) 1089, 1105
EcoT38I GRGCYC 1 cut(s) 1781
ErhI CCWWGG 3 cut(s) 260, 531, 1728
FbaI TGATCA 1 cut(s) 22
FblI GTMKAC 1 cut(s) 1739
FokI GGATG 2 cut(s) 543, 1682
FriOI GRGCYC 1 cut(s) 1781
FspBI CTAG 8 cut(s) 213, 441, 771, 930, 1427, 1463, 1806, 2010
GlaI GCGC 6 cut(s) 698, 700, 1535, 1754, 1756, 1764
GsaI CCCAGC 1 cut(s) 1668
GsuI CTGGAG 1 cut(s) 1619
HaeIII GGCC 7 cut(s) 72, 615, 675, 730, 810, 992, 1613
HapII CCGG 1 cut(s) 1749
HgaI GACGC 1 cut(s) 323
HhaI GCGC 6 cut(s) 699, 701, 1536, 1755, 1757, 1765
Hin6I GCGC 6 cut(s) 697, 699, 1534, 1753, 1755, 1763
HinP1I GCGC 6 cut(s) 697, 699, 1534, 1753, 1755, 1763
HincII GTYRAC 1 cut(s) 1443
HindII GTYRAC 1 cut(s) 1443
HindIII AAGCTT 1 cut(s) 680
HpaI GTTAAC 1 cut(s) 1443
HpaII CCGG 1 cut(s) 1749
HphI GGTGA 6 cut(s) 248, 257, 333, 468, 1469, 1823
Hpy166II GTNNAC 7 cut(s) 471, 590, 1045, 1351, 1417, 1443, 1740
Hpy188III TCNNGA 9 cut(s) 127, 431, 602, 880, 1148, 1196, 1598, 1703, 1770
Hpy8I GTNNAC 7 cut(s) 471, 590, 1045, 1351, 1417, 1443, 1740
HpyAV CCTTC 7 cut(s) 115, 405, 409, 741, 791, 800, 903
HpyCH4III ACNGT 5 cut(s) 35, 646, 968, 1213, 1873
HspAI GCGC 6 cut(s) 697, 699, 1534, 1753, 1755, 1763
KpnI GGTACC 1 cut(s) 1881
Ksp22I TGATCA 1 cut(s) 22
KspAI GTTAAC 1 cut(s) 1443
Kzo9I GATC 6 cut(s) 22, 247, 1072, 1423, 1525, 1999
LmnI GCTCC 1 cut(s) 101
LweI GCATC 6 cut(s) 26, 48, 942, 1112, 1232, 1943
MaeI CTAG 8 cut(s) 213, 441, 771, 930, 1427, 1463, 1806, 2010
MaeIII GTNAC 5 cut(s) 339, 505, 640, 751, 1811
MalI GATC 6 cut(s) 24, 249, 1074, 1425, 1527, 2001
MboI GATC 6 cut(s) 22, 247, 1072, 1423, 1525, 1999
MboII GAAGA 8 cut(s) 590, 786, 804, 914, 1249, 1340, 1641, 1959
MfeI CAATTG 1 cut(s) 995
MflI RGATCY 2 cut(s) 1423, 1999
MhlI GDGCHC 2 cut(s) 592, 1781
MlsI TGGCCA 1 cut(s) 615
MluNI TGGCCA 1 cut(s) 615
MlyI GAGTC 5 cut(s) 536, 786, 870, 1324, 1936
MmeI TCCRAC 2 cut(s) 223, 365
Mox20I TGGCCA 1 cut(s) 615
Mph1103I ATGCAT 2 cut(s) 1089, 1105
MscI TGGCCA 1 cut(s) 615
MseI TTAA 4 cut(s) 270, 513, 1442, 1643
Msp20I TGGCCA 1 cut(s) 615
MspA1I CMGCKG 1 cut(s) 545
MspI CCGG 1 cut(s) 1749
MspR9I CCNGG 4 cut(s) 500, 617, 1448, 1750
MunI CAATTG 1 cut(s) 995
MvaI CCWGG 3 cut(s) 500, 617, 1448
MvnI CGCG 4 cut(s) 334, 562, 699, 1755
NciI CCSGG 1 cut(s) 1750
NcoI CCATGG 2 cut(s) 260, 1728
NdeII GATC 6 cut(s) 22, 247, 1072, 1423, 1525, 1999
NheI GCTAGC 1 cut(s) 929
NlaIV GGNNCC 6 cut(s) 729, 896, 920, 1114, 1879, 1912
NmeAIII GCCGAG 1 cut(s) 191
NmuCI GTSAC 3 cut(s) 339, 640, 1811
NsiI ATGCAT 2 cut(s) 1089, 1105
NspI RCATGY 3 cut(s) 670, 1027, 1091
PaeI GCATGC 2 cut(s) 1027, 1091
PaeR7I CTCGAG 1 cut(s) 878
PaqCI CACCTGC 1 cut(s) 1898
PauI GCGCGC 2 cut(s) 697, 1753
PfeI GAWTC 8 cut(s) 517, 551, 1004, 1130, 1144, 1151, 1542, 1634
PflFI GACNNNGTC 1 cut(s) 526
PflMI CCANNNNNTGG 2 cut(s) 988, 1949
PleI GAGTC 5 cut(s) 535, 785, 870, 1323, 1935
PpsI GAGTC 5 cut(s) 535, 785, 870, 1323, 1935
PpuMI RGGWCCY 2 cut(s) 895, 1288
Psp124BI GAGCTC 1 cut(s) 1781
Psp5II RGGWCCY 2 cut(s) 895, 1288
Psp6I CCWGG 3 cut(s) 498, 615, 1446
PspFI CCCAGC 1 cut(s) 1664
PspGI CCWGG 3 cut(s) 498, 615, 1446
PspN4I GGNNCC 6 cut(s) 729, 896, 920, 1114, 1879, 1912
PspPI GGNCC 5 cut(s) 728, 895, 991, 1288, 1611
PspPPI RGGWCCY 2 cut(s) 895, 1288
PstI CTGCAG 3 cut(s) 550, 1794, 1909
PsuI RGATCY 2 cut(s) 1423, 1999
PsyI GACNNNGTC 1 cut(s) 526
PteI GCGCGC 2 cut(s) 697, 1753
PvuII CAGCTG 1 cut(s) 545
RsaI GTAC 7 cut(s) 217, 365, 625, 1352, 1418, 1879, 1986
RsaNI GTAC 7 cut(s) 216, 364, 624, 1351, 1417, 1878, 1985
SacI GAGCTC 1 cut(s) 1781
SaqAI TTAA 4 cut(s) 270, 513, 1442, 1643
Sau3AI GATC 6 cut(s) 22, 247, 1072, 1423, 1525, 1999
Sau96I GGNCC 5 cut(s) 728, 895, 991, 1288, 1611
ScaI AGTACT 2 cut(s) 217, 365
SchI GAGTC 5 cut(s) 536, 786, 870, 1324, 1936
ScrFI CCNGG 4 cut(s) 500, 617, 1448, 1750
SduI GDGCHC 2 cut(s) 592, 1781
SfaNI GCATC 6 cut(s) 26, 48, 942, 1112, 1232, 1943
SfcI CTRYAG 4 cut(s) 546, 1560, 1790, 1905
Sfr274I CTCGAG 1 cut(s) 878
SinI GGWCC 2 cut(s) 895, 1288
SlaI CTCGAG 1 cut(s) 878
SmlI CTYRAG 1 cut(s) 878
SmoI CTYRAG 1 cut(s) 878
SpeI ACTAGT 2 cut(s) 212, 2009
SphI GCATGC 2 cut(s) 1027, 1091
SsiI CCGC 2 cut(s) 332, 560
SspMI CTAG 8 cut(s) 213, 441, 771, 930, 1427, 1463, 1806, 2010
SstI GAGCTC 1 cut(s) 1781
StyD4I CCNGG 4 cut(s) 498, 615, 1446, 1748
StyI CCWWGG 3 cut(s) 260, 531, 1728
TaaI ACNGT 5 cut(s) 35, 646, 968, 1213, 1873
TaqI TCGA 3 cut(s) 633, 879, 1128
TaqII GACCGA 2 cut(s) 539, 753
TatI WGTACW 3 cut(s) 215, 363, 1416
TauI GCSGC 1 cut(s) 334
TfiI GAWTC 8 cut(s) 517, 551, 1004, 1130, 1144, 1151, 1542, 1634
Tru1I TTAA 4 cut(s) 270, 513, 1442, 1643
Tru9I TTAA 4 cut(s) 270, 513, 1442, 1643
TscAI CASTG 5 cut(s) 91, 1189, 1794, 1876, 1901
TseFI GTSAC 3 cut(s) 339, 640, 1811
Tsp45I GTSAC 3 cut(s) 339, 640, 1811
TspDTI ATGAA 7 cut(s) 397, 471, 1047, 1143, 1383, 1815, 1974
TspRI CASTG 5 cut(s) 91, 1189, 1794, 1876, 1901
Tth111I GACNNNGTC 1 cut(s) 526
Van91I CCANNNNNTGG 2 cut(s) 988, 1949
VneI GTGCAC 1 cut(s) 588
VpaK11BI GGWCC 2 cut(s) 895, 1288
XapI RAATTY 2 cut(s) 707, 1035
XceI RCATGY 3 cut(s) 670, 1027, 1091
XhoI CTCGAG 1 cut(s) 878
XmiI GTMKAC 1 cut(s) 1739
XspI CTAG 8 cut(s) 213, 441, 771, 930, 1427, 1463, 1806, 2010
ZrmI AGTACT 2 cut(s) 217, 365
Zsp2I ATGCAT 2 cut(s) 1089, 1105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.