Rmu_sc0002561.1_g000002

pre-mRNA 3'-splice site binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002561.1
OG0000360
Physical Location & Seq
Forward (+)
3191 .. 6953
3763 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002561.1_g000002.1.cds

Sequence Viewer

Length: 2559 bp
atgggcttcaaagtccaatttagtatatttcttgagtacccaacatgggcttcaaagcataagagagcagtcaatggctgtggtcaaccaatagtcaaacaaacaagagctacatgggcttcaaagtccaatttagaagctaaacaactccttcacccccaagttctcctccgactcctcatccataaaatctcatcggcgtctctcacagtttccaacctctctccaagaagaaggaaagagcttttgacccaggtgatcactccgaacatgttttcgtctatggatactgcatctgttcaaggttctgttgctggtgggcttggggatcctgatcgaattttttttggtggattcccttactactttaaagaagaacagatcagggagttggtagagtccttcggaccccttcgcagttttgaactagtgacagatggaggcagaggaaacaactcagttgctgcccattgcgtttaccaggatgtttcagttacagacattgcttgtcatggcctgaatgggattcgaatcggtgcccagacactgattgctaggcgtgctaatcatggtgccaaccagcagccggaaccacattcttcttctcaagaaaattgcagtatttgctggtgtagcctgacaactggccaaggccaagcgatctttactgctgagtgctcccaccgtttccactacccttgtattgccaacaatgtccagcatggaaatctttactgccctgtctgcagagtaaaatgggacaagaataatgtacctttccaagttcctcctcctcaacagaccaggatccctttcaggcagttcccaatgctgcagcaaccccattggaattatcctcacctcccatatcagcagccgcattggaatcatcctcagtacccatttgagcagcaaccccaggaccctcctgagccacttaccttctctgatgatgaacctctccagtcattgacctcacctgttcaatcctcctcccaccagaatgtgacagttacgacttatgcagagtactctgctatctctgctgcggagtctagctcaaagtttcctgtcctggtcagcatccgtgcaccaccccttaaggattctgagggccatggtcgtactcccattgaccttgtaacagttctggatgtaagtggcagcatgactggcgcgaagcttaaccttgtcaagcgtgctgtcaaatttgtcatagaaaacttggggtcttcagacagactttctattgtctcattctcatcaaattctaagagagttcttcctctccaaaggatgactgctgatggccgtgaaagtgccatactagccatcgagtctcttagagcaggtggtggaactaacattgccgaagcactcaagaaaggaattaaggtccttgaagatcgaagggaaaggaacccagttgctagcatcatcctcttatccgatggccaagataactactgtcgaagtggaagccaaattttgaaccaattgcctgcttccattcgttctagtgacatgcaacaggaaatcccagtccatgcatttgggtttggccaagaccatgatgcaagcattatgcatgctatttctgatgcatccggtggtaccttttcattcattgaatctgttggcatgatacaagatggttttgctttgtgcattggtggtcttctcagtgtagtggctcaggagcttcgcctcacagtaaggtcagcatcagctggggtgaagattgtttcaataccatcagggagacatgtgagccagatttctgatgagggccagcaaggtgtagttgatattggaaatatgtatgcagaagaggagaaacaatttctggtctacctgttagttccaccctcagcttcacatgttaagatgccattgttagatgtaacatgcgtgtacaaagatcttgcttcaaatgagttgatgcaggtgcaaggtgagagagtagagatactgagacctgaggtttgttcccctgcagaaaagacagtgtctttggaggttgatcgacagcggaacagaattgtggtggctgaaactattgcagaggcacaaaggttggctgagatgggaaacctggaaggtgcccgggcacttttggctgaacgaagagcaactgtcttagcatcagcagcagctcaagctggggatggtctcagtaatttgtttgaaactgagcttggagaagtcatggagagaatggcatctatggacttgtacgctaagtcagggcgtgcttacgctctcgcagggatgagttctcactctcgacagagggcttcagctaggggtgacacaatgacctcatgtctctttggtgcctccaaccatgtgaatttctccagcggtggcaatgccttaatgcagcagtcgggtgcctctgcttgttttggccgccctgcaggtttcagtggtgctagtttttcatccggtgccgctccaggtggaagtcaatgctcttctgcagttggtgcttttgctacacctgctatggttagaatggtagaaaaatcacagaagagcaatcaatctgggcaataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
HA2 Helicase_C OB_NTP_bind RRM_1 S1

Protein Analysis

852

Amino Acids

92.34

Weight (kDa)

7.31

Isoelectric Point (pI)

50.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000360)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11691 FvH4_3g11693 FvH4_3g11740 FvH4_3g11750 FvH4_3g11770 FvH4_3g11771 FvH4_3g11772 FvH4_3g11780
malus_domestica MD10G1239000.v1.1
prunus_persica Prupe.4G105400_v2.0.a1
pyrus_communis pycom10g20000
rosa_chinensis RchiOBHm_Chr5g0018981 RchiOBHm_Chr5g0018991 RchiOBHm_Chr5g0019101 RchiOBHm_Chr5g0019111 RchiOBHm_Chr5g0019121 RchiOBHm_Chr5g0019151 RchiOBHm_Chr5g0019161 RchiOBHm_Chr5g0019171 RchiOBHm_Chr5g0019181 RchiOBHm_Chr5g0019201
rosa_laevigata RLG00000032411 RLG00000032418 RLG00000032419 RLG00000032420 RLG00000032421 RLG00000032423 RLG00000032424 RLG00000032426
rosa_multiflora Rmu_co8475613.1_g000001 Rmu_co8503587.1_g000001 Rmu_sc0000563.1_g000023 Rmu_sc0000563.1_g000027 Rmu_sc0002142.1_g000001 Rmu_sc0002142.1_g000003 Rmu_sc0002142.1_g000004 Rmu_sc0002142.1_g000011 Rmu_sc0002142.1_g000022 Rmu_sc0002561.1_g000002 Rmu_sc0042836.1_g000001
rosa_roxburghii Rroxscaffold_1G00058790 Rroxscaffold_1G00058810 Rroxscaffold_1G00058820 Rroxscaffold_1G00058840 Rroxscaffold_1G00058850 Rroxscaffold_1G00058860 Rroxscaffold_1G00058880 Rroxscaffold_1G00058910 Rroxscaffold_1G00074090 Rroxscaffold_1G00074110 Rroxscaffold_1G00074120 Rroxscaffold_1G00074130 Rroxscaffold_1G00074150
rosa_rugosa Rorug05G0046800 Rorug05G0047200 Rorug05G0047300 Rorug05G0047400 Rorug05G0047600 Rorug05G0047600 Rorug05G0047800 Rorug05G0047900 Rorug05G0048000 Rorug05G0048100 Rorug05G0048100 Rorug05G0048200 Rorug05G0048300
rosa_samantha Rh4DG102800 Rh5AG138700 Rh5AG138900 Rh5AG139000 Rh5AG139100 Rh5AG139200 Rh5AG139400 Rh5AG139500 Rh5AG139600 Rh5BG137400 Rh5BG137600 Rh5BG137800 Rh5BG138100 Rh5BG138200 Rh5BG138300 Rh5BG138500 Rh5CG148800 Rh5CG148900 Rh5CG149200 Rh5CG149300 Rh5CG149400 Rh5CG149600 Rh5CG149700 Rh5CG150000 Rh5DG138200 Rh5DG138300 Rh5DG138500 Rh5DG138600 Rh5DG138700 Rh5DG138900 Rh5DG139000 Rh5DG139200
rosa_wichuraiana Rw5G012290 Rw5G012330 Rw5G012340 Rw5G012350 Rw5G012370 Rw5G012380 Rw5G012390 Rw5G012410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 1340, 1936, 2512
Acc36I ACCTGC 4 cut(s) 1340, 1936, 2411, 2512
Acc65I GGTACC 1 cut(s) 1613
AccB1I GGYRCC 7 cut(s) 536, 572, 1613, 2102, 2336, 2393, 2450
AccBSI CCGCTC 1 cut(s) 2456
AccI GTMKAC 1 cut(s) 1851
AccII CGCG 1 cut(s) 1178
AciI CCGC 6 cut(s) 878, 1049, 2032, 2364, 2413, 2454
AclWI GGATC 4 cut(s) 323, 336, 802, 815
AcoI YGGCCR 5 cut(s) 646, 1309, 1453, 1561, 2410
AcsI RAATTY 5 cut(s) 339, 1208, 1267, 1485, 2353
AcuI CTGAAG 2 cut(s) 1218, 2283
AcyI GRCGYC 1 cut(s) 200
AfaI GTAC 8 cut(s) 38, 774, 899, 1031, 1126, 1615, 1916, 2237
AfiI CCNNNNNNNGG 2 cut(s) 46, 586
AflII CTTAAG 1 cut(s) 1100
AflIII ACRYGT 3 cut(s) 270, 1765, 1879
AhdI GACNNNNNGTC 1 cut(s) 2325
AhlI ACTAGT 1 cut(s) 427
AjnI CCWGG 7 cut(s) 252, 480, 803, 918, 1074, 2094, 2458
AjuI GAANNNNNNNTTGG 4 cut(s) 34, 66, 2181, 2213
Alw21I GWGCWC 2 cut(s) 680, 1093
Alw26I GTCTC 7 cut(s) 207, 1258, 1344, 1756, 1969, 2177, 2333
Alw44I GTGCAC 1 cut(s) 1089
AlwI GGATC 4 cut(s) 323, 336, 802, 815
AlwNI CAGNNNCTG 2 cut(s) 464, 547
Ama87I CYCGRG 1 cut(s) 2106
AoxI GGCC 9 cut(s) 514, 646, 652, 1114, 1309, 1453, 1561, 1789, 2410
ApaLI GTGCAC 1 cut(s) 1089
ApoI RAATTY 5 cut(s) 339, 1208, 1267, 1485, 2353
ArsI GACNNNNNNTTYG 4 cut(s) 1968, 1996, 2000, 2028
Asp718I GGTACC 1 cut(s) 1613
AspLEI GCGC 1 cut(s) 1178
AspS9I GGNCC 5 cut(s) 407, 922, 1114, 1396, 1789
AsuC2I CCSGG 2 cut(s) 2107, 2108
AsuHPI GGTGA 7 cut(s) 146, 268, 851, 969, 1747, 1967, 2321
AsuII TTCGAA 1 cut(s) 529
AsuNHI GCTAGC 1 cut(s) 1430
AvaI CYCGRG 1 cut(s) 2106
AvaII GGWCC 3 cut(s) 407, 922, 1396
AxyI CCTNAGG 1 cut(s) 1980
BaeGI GKGCMC 4 cut(s) 541, 1093, 2107, 2113
BalI TGGCCA 3 cut(s) 648, 1455, 1563
BamHI GGATCC 2 cut(s) 328, 807
BanI GGYRCC 7 cut(s) 536, 572, 1613, 2102, 2336, 2393, 2450
BbsI GAAGAC 2 cut(s) 1224, 1670
Bbv12I GWGCWC 2 cut(s) 680, 1093
BbvCI CCTCAGC 1 cut(s) 1870
BccI CCATC 8 cut(s) 431, 1301, 1340, 1445, 1646, 1762, 2080, 2162
BceAI ACGGC 1 cut(s) 1296
BciT130I CCWGG 7 cut(s) 254, 482, 805, 920, 1076, 2096, 2460
BciVI GTATCC 1 cut(s) 280
BclI TGATCA 1 cut(s) 258
BcnI CCSGG 2 cut(s) 2107, 2108
BcoDI GTCTC 7 cut(s) 207, 1258, 1344, 1756, 1969, 2177, 2333
BcuI ACTAGT 1 cut(s) 427
BfaI CTAG 8 cut(s) 428, 555, 1056, 1328, 1431, 1518, 2304, 2436
BfmI CTRYAG 5 cut(s) 745, 833, 1995, 2418, 2481
BfrI CTTAAG 1 cut(s) 1100
BfuAI ACCTGC 4 cut(s) 1340, 1936, 2411, 2512
BfuI GTATCC 1 cut(s) 280
BglII AGATCT 1 cut(s) 1921
BmcAI AGTACT 1 cut(s) 1031
Bme1390I CCNGG 9 cut(s) 254, 482, 805, 920, 1076, 2096, 2107, 2108, 2460
Bme18I GGWCC 3 cut(s) 407, 922, 1396
BmeRI GACNNNNNGTC 1 cut(s) 2325
BmeT110I CYCGRG 1 cut(s) 2106
BmgT120I GGNCC 5 cut(s) 407, 922, 1114, 1396, 1789
BmrFI CCNGG 9 cut(s) 254, 482, 805, 920, 1076, 2096, 2107, 2108, 2460
BmrI ACTGGG 2 cut(s) 1418, 1535
BmtI GCTAGC 1 cut(s) 1434
BmuI ACTGGG 2 cut(s) 1418, 1535
BpiI GAAGAC 2 cut(s) 1224, 1670
BplI GAGNNNNNCTC 2 cut(s) 1043, 1075
BpmI CTGGAG 3 cut(s) 947, 2344, 2442
Bpu10I CCTNAGC 3 cut(s) 930, 1695, 1870
Bpu14I TTCGAA 1 cut(s) 529
BpuEI CTTGAG 4 cut(s) 53, 591, 1364, 2142
BpuMI CCSGG 2 cut(s) 2107, 2108
BsaBI GATNNNNATC 2 cut(s) 333, 530
BsaHI GRCGYC 1 cut(s) 200
BsaI GGTCTC 2 cut(s) 1969, 2177
BsaJI CCNNGG 5 cut(s) 252, 649, 918, 1117, 2106
BsaWI WCCGGW 2 cut(s) 1607, 2447
Bsc4I CCNNNNNNNGG 2 cut(s) 46, 586
Bse1I ACTGG 5 cut(s) 649, 964, 1177, 1424, 1541
Bse21I CCTNAGG 1 cut(s) 1980
Bse3DI GCAATG 4 cut(s) 469, 501, 1365, 2377
Bse8I GATNNNNATC 2 cut(s) 333, 530
BseBI CCWGG 7 cut(s) 254, 482, 805, 920, 1076, 2096, 2460
BseDI CCNNGG 5 cut(s) 252, 649, 918, 1117, 2106
BseJI GATNNNNATC 2 cut(s) 333, 530
BseLI CCNNNNNNNGG 2 cut(s) 46, 586
BseMI GCAATG 4 cut(s) 469, 501, 1365, 2377
BseNI ACTGG 5 cut(s) 649, 964, 1177, 1424, 1541
BseRI GAGGAG 6 cut(s) 158, 167, 780, 783, 982, 1847
BseSI GKGCMC 4 cut(s) 541, 1093, 2107, 2113
BseYI CCCAGC 2 cut(s) 1730, 2162
Bsh1236I CGCG 1 cut(s) 1178
BshFI GGCC 9 cut(s) 516, 648, 654, 1116, 1311, 1455, 1563, 1791, 2412
BshNI GGYRCC 7 cut(s) 536, 572, 1613, 2102, 2336, 2393, 2450
BsiHKAI GWGCWC 2 cut(s) 680, 1093
BsiHKCI CYCGRG 1 cut(s) 2106
BsiSI CCGG 4 cut(s) 587, 1608, 2107, 2448
BslFI GGGAC 1 cut(s) 773
BslI CCNNNNNNNGG 2 cut(s) 46, 586
BsmAI GTCTC 7 cut(s) 207, 1258, 1344, 1756, 1969, 2177, 2333
BsmBI CGTCTC 1 cut(s) 207
BsmFI GGGAC 1 cut(s) 773
BsnI GGCC 9 cut(s) 516, 648, 654, 1116, 1311, 1455, 1563, 1791, 2412
Bso31I GGTCTC 2 cut(s) 1969, 2177
BsoBI CYCGRG 1 cut(s) 2106
Bsp119I TTCGAA 1 cut(s) 529
Bsp1286I GDGCHC 5 cut(s) 541, 680, 1093, 2107, 2113
Bsp1407I TGTACA 1 cut(s) 1914
Bsp143I GATC 9 cut(s) 258, 328, 334, 381, 660, 807, 1405, 1921, 2023
Bsp19I CCATGG 1 cut(s) 1117
BspACI CCGC 6 cut(s) 878, 1049, 2032, 2364, 2413, 2454
BspANI GGCC 9 cut(s) 516, 648, 654, 1116, 1311, 1455, 1563, 1791, 2412
BspFNI CGCG 1 cut(s) 1178
BspMAI CTGCAG 5 cut(s) 749, 837, 1999, 2422, 2485
BspMI ACCTGC 4 cut(s) 1340, 1936, 2411, 2512
BspOI GCTAGC 1 cut(s) 1434
BspPI GGATC 4 cut(s) 323, 336, 802, 815
BspQI GCTCTTC 3 cut(s) 2122, 2482, 2531
BspT104I TTCGAA 1 cut(s) 529
BspT107I GGYRCC 7 cut(s) 536, 572, 1613, 2102, 2336, 2393, 2450
BspTI CTTAAG 1 cut(s) 1100
BspTNI GGTCTC 2 cut(s) 1969, 2177
BsrBI CCGCTC 1 cut(s) 2456
BsrDI GCAATG 4 cut(s) 469, 501, 1365, 2377
BsrGI TGTACA 1 cut(s) 1914
BsrI ACTGG 5 cut(s) 649, 964, 1177, 1424, 1541
BssECI CCNNGG 5 cut(s) 252, 649, 918, 1117, 2106
BssMI GATC 9 cut(s) 258, 328, 334, 381, 660, 807, 1405, 1921, 2023
BssNI GRCGYC 1 cut(s) 200
BssT1I CCWWGG 2 cut(s) 649, 1117
Bst2UI CCWGG 7 cut(s) 254, 482, 805, 920, 1076, 2096, 2460
Bst4CI ACNGT 8 cut(s) 211, 686, 1012, 1147, 1469, 1714, 2008, 2137
Bst6I CTCTTC 4 cut(s) 1824, 2122, 2482, 2531
BstACI GRCGYC 1 cut(s) 200
BstAFI CTTAAG 1 cut(s) 1100
BstAPI GCANNNNNTGC 2 cut(s) 624, 2489
BstAUI TGTACA 1 cut(s) 1914
BstBI TTCGAA 1 cut(s) 529
BstC8I GCNNGC 8 cut(s) 561, 1200, 1432, 1503, 1579, 1590, 1793, 2253
BstDSI CCRYGG 1 cut(s) 1117
BstFNI CGCG 1 cut(s) 1178
BstHHI GCGC 1 cut(s) 1178
BstKTI GATC 9 cut(s) 261, 331, 337, 384, 663, 810, 1408, 1924, 2026
BstMAI GTCTC 7 cut(s) 207, 1258, 1344, 1756, 1969, 2177, 2333
BstMBI GATC 9 cut(s) 258, 328, 334, 381, 660, 807, 1405, 1921, 2023
BstNI CCWGG 7 cut(s) 254, 482, 805, 920, 1076, 2096, 2460
BstNSI RCATGY 6 cut(s) 274, 1528, 1592, 1769, 1883, 1911
BstSCI CCNGG 9 cut(s) 252, 480, 803, 918, 1074, 2094, 2105, 2106, 2458
BstSFI CTRYAG 5 cut(s) 745, 833, 1995, 2418, 2481
BstSLI GKGCMC 4 cut(s) 541, 1093, 2107, 2113
BstUI CGCG 1 cut(s) 1178
BstV2I GAAGAC 2 cut(s) 1224, 1670
BstX2I RGATCY 3 cut(s) 328, 807, 1921
BstXI CCANNNNNNTGG 1 cut(s) 1553
BstYI RGATCY 3 cut(s) 328, 807, 1921
Bsu36I CCTNAGG 1 cut(s) 1980
BsuI GTATCC 1 cut(s) 280
BsuRI GGCC 9 cut(s) 516, 648, 654, 1116, 1311, 1455, 1563, 1791, 2412
BtgI CCRYGG 1 cut(s) 1117
BtsIMutI CAGTG 4 cut(s) 545, 1690, 2013, 2434
BveI ACCTGC 4 cut(s) 1340, 1936, 2411, 2512
Cac8I GCNNGC 8 cut(s) 561, 1200, 1432, 1503, 1579, 1590, 1793, 2253
CaiI CAGNNNCTG 2 cut(s) 464, 547
CfoI GCGC 1 cut(s) 1178
Cfr13I GGNCC 5 cut(s) 407, 922, 1114, 1396, 1789
Cfr9I CCCGGG 1 cut(s) 2106
CseI GACGC 1 cut(s) 189
Csp6I GTAC 8 cut(s) 37, 773, 898, 1030, 1125, 1614, 1915, 2236
CspCI CAANNNNNGTGG 4 cut(s) 61, 96, 680, 715
CviQI GTAC 8 cut(s) 37, 773, 898, 1030, 1125, 1614, 1915, 2236
DpnI GATC 9 cut(s) 260, 330, 336, 383, 662, 809, 1407, 1923, 2025
DpnII GATC 9 cut(s) 258, 328, 334, 381, 660, 807, 1405, 1921, 2023
DraI TTTAAA 1 cut(s) 370
DriI GACNNNNNGTC 1 cut(s) 2325
EaeI YGGCCR 5 cut(s) 646, 1309, 1453, 1561, 2410
Eam1104I CTCTTC 4 cut(s) 1824, 2122, 2482, 2531
Eam1105I GACNNNNNGTC 1 cut(s) 2325
EarI CTCTTC 4 cut(s) 1824, 2122, 2482, 2531
Eco130I CCWWGG 2 cut(s) 649, 1117
Eco31I GGTCTC 2 cut(s) 1969, 2177
Eco47I GGWCC 3 cut(s) 407, 922, 1396
Eco57I CTGAAG 2 cut(s) 1218, 2283
Eco81I CCTNAGG 1 cut(s) 1980
Eco88I CYCGRG 1 cut(s) 2106
EcoO109I RGGNCCY 2 cut(s) 922, 1396
EcoRII CCWGG 7 cut(s) 252, 480, 803, 918, 1074, 2094, 2458
EcoT14I CCWWGG 2 cut(s) 649, 1117
EcoT22I ATGCAT 3 cut(s) 1552, 1590, 1606
ErhI CCWWGG 2 cut(s) 649, 1117
Esp3I CGTCTC 1 cut(s) 207
FaqI GGGAC 1 cut(s) 773
FbaI TGATCA 1 cut(s) 258
FblI GTMKAC 1 cut(s) 1851
FspBI CTAG 8 cut(s) 428, 555, 1056, 1328, 1431, 1518, 2304, 2436
GlaI GCGC 1 cut(s) 1177
GsaI CCCAGC 2 cut(s) 1734, 2166
GsuI CTGGAG 3 cut(s) 947, 2344, 2442
HaeIII GGCC 9 cut(s) 516, 648, 654, 1116, 1311, 1455, 1563, 1791, 2412
HapII CCGG 4 cut(s) 587, 1608, 2107, 2448
HgaI GACGC 1 cut(s) 189
HhaI GCGC 1 cut(s) 1178
Hin1I GRCGYC 1 cut(s) 200
Hin6I GCGC 1 cut(s) 1176
HinP1I GCGC 1 cut(s) 1176
HincII GTYRAC 1 cut(s) 86
HindII GTYRAC 1 cut(s) 86
HindIII AAGCTT 1 cut(s) 1181
HpaII CCGG 4 cut(s) 587, 1608, 2107, 2448
HphI GGTGA 7 cut(s) 146, 268, 851, 969, 1747, 1967, 2321
Hpy166II GTNNAC 5 cut(s) 86, 478, 1091, 1852, 1915
Hpy188I TCNGA 9 cut(s) 173, 267, 407, 949, 1111, 1237, 1450, 1600, 1783
Hpy188III TCNNGA 8 cut(s) 32, 332, 608, 929, 1151, 1381, 1697, 2286
Hpy8I GTNNAC 5 cut(s) 86, 478, 1091, 1852, 1915
HpyAV CCTTC 7 cut(s) 161, 228, 412, 422, 952, 1404, 2093
HpyCH4III ACNGT 8 cut(s) 211, 686, 1012, 1147, 1469, 1714, 2008, 2137
Hsp92I GRCGYC 1 cut(s) 200
HspAI GCGC 1 cut(s) 1176
KpnI GGTACC 1 cut(s) 1617
Ksp22I TGATCA 1 cut(s) 258
Kzo9I GATC 9 cut(s) 258, 328, 334, 381, 660, 807, 1405, 1921, 2023
LguI GCTCTTC 3 cut(s) 2122, 2482, 2531
LmnI GCTCC 3 cut(s) 683, 1699, 2461
MaeI CTAG 8 cut(s) 428, 555, 1056, 1328, 1431, 1518, 2304, 2436
MaeIII GTNAC 8 cut(s) 430, 493, 1006, 1012, 1141, 1520, 1903, 2309
MalI GATC 9 cut(s) 260, 330, 336, 383, 662, 809, 1407, 1923, 2025
MbiI CCGCTC 1 cut(s) 2456
MboI GATC 9 cut(s) 258, 328, 334, 381, 660, 807, 1405, 1921, 2023
MfeI CAATTG 1 cut(s) 1496
MflI RGATCY 3 cut(s) 328, 807, 1921
MhlI GDGCHC 5 cut(s) 541, 680, 1093, 2107, 2113
MlsI TGGCCA 3 cut(s) 648, 1455, 1563
MluNI TGGCCA 3 cut(s) 648, 1455, 1563
MlyI GAGTC 4 cut(s) 168, 407, 1061, 1346
MmeI TCCRAC 3 cut(s) 196, 240, 2367
Mox20I TGGCCA 3 cut(s) 648, 1455, 1563
Mph1103I ATGCAT 3 cut(s) 1552, 1590, 1606
MscI TGGCCA 3 cut(s) 648, 1455, 1563
MseI TTAA 6 cut(s) 369, 1101, 1185, 1392, 1884, 2378
MslI CAYNNNNRTG 1 cut(s) 1002
Msp20I TGGCCA 3 cut(s) 648, 1455, 1563
MspA1I CMGCKG 3 cut(s) 1730, 2032, 2364
MspCI CTTAAG 1 cut(s) 1100
MspI CCGG 4 cut(s) 587, 1608, 2107, 2448
MspR9I CCNGG 9 cut(s) 254, 482, 805, 920, 1076, 2096, 2107, 2108, 2460
MunI CAATTG 1 cut(s) 1496
MvaI CCWGG 7 cut(s) 254, 482, 805, 920, 1076, 2096, 2460
MvnI CGCG 1 cut(s) 1178
NciI CCSGG 2 cut(s) 2107, 2108
NcoI CCATGG 1 cut(s) 1117
NdeII GATC 9 cut(s) 258, 328, 334, 381, 660, 807, 1405, 1921, 2023
NheI GCTAGC 1 cut(s) 1430
NmuCI GTSAC 4 cut(s) 430, 1006, 1520, 2309
NsiI ATGCAT 3 cut(s) 1552, 1590, 1606
NspI RCATGY 6 cut(s) 274, 1528, 1592, 1769, 1883, 1911
NspV TTCGAA 1 cut(s) 529
PaeI GCATGC 1 cut(s) 1592
PaqCI CACCTGC 3 cut(s) 1340, 1936, 2512
PciI ACATGT 3 cut(s) 270, 1765, 1879
PciSI GCTCTTC 3 cut(s) 2122, 2482, 2531
PfeI GAWTC 6 cut(s) 354, 526, 531, 886, 1106, 1631
PflFI GACNNNGTC 1 cut(s) 2008
PleI GAGTC 4 cut(s) 168, 406, 1060, 1345
PpsI GAGTC 4 cut(s) 168, 406, 1060, 1345
PpuMI RGGWCCY 2 cut(s) 922, 1396
PscI ACATGT 3 cut(s) 270, 1765, 1879
Psp5II RGGWCCY 2 cut(s) 922, 1396
Psp6I CCWGG 7 cut(s) 252, 480, 803, 918, 1074, 2094, 2458
PspFI CCCAGC 2 cut(s) 1730, 2162
PspGI CCWGG 7 cut(s) 252, 480, 803, 918, 1074, 2094, 2458
PspPI GGNCC 5 cut(s) 407, 922, 1114, 1396, 1789
PspPPI RGGWCCY 2 cut(s) 922, 1396
PstI CTGCAG 5 cut(s) 749, 837, 1999, 2422, 2485
PstNI CAGNNNCTG 2 cut(s) 464, 547
PsuI RGATCY 3 cut(s) 328, 807, 1921
PsyI GACNNNGTC 1 cut(s) 2008
PvuII CAGCTG 1 cut(s) 1730
RsaI GTAC 8 cut(s) 38, 774, 899, 1031, 1126, 1615, 1916, 2237
RsaNI GTAC 8 cut(s) 37, 773, 898, 1030, 1125, 1614, 1915, 2236
RseI CAYNNNNRTG 1 cut(s) 1002
SapI GCTCTTC 3 cut(s) 2122, 2482, 2531
SaqAI TTAA 6 cut(s) 369, 1101, 1185, 1392, 1884, 2378
Sau3AI GATC 9 cut(s) 258, 328, 334, 381, 660, 807, 1405, 1921, 2023
Sau96I GGNCC 5 cut(s) 407, 922, 1114, 1396, 1789
SbfI CCTGCAGG 1 cut(s) 2422
ScaI AGTACT 1 cut(s) 1031
SchI GAGTC 4 cut(s) 168, 407, 1061, 1346
ScrFI CCNGG 9 cut(s) 254, 482, 805, 920, 1076, 2096, 2107, 2108, 2460
SdaI CCTGCAGG 1 cut(s) 2422
SduI GDGCHC 5 cut(s) 541, 680, 1093, 2107, 2113
SfcI CTRYAG 5 cut(s) 745, 833, 1995, 2418, 2481
SfuI TTCGAA 1 cut(s) 529
SinI GGWCC 3 cut(s) 407, 922, 1396
SmaI CCCGGG 1 cut(s) 2108
SmiMI CAYNNNNRTG 1 cut(s) 1002
SmlI CTYRAG 5 cut(s) 32, 606, 1100, 1379, 2157
SmoI CTYRAG 5 cut(s) 32, 606, 1100, 1379, 2157
SpeI ACTAGT 1 cut(s) 427
SphI GCATGC 1 cut(s) 1592
SrfI GCCCGGGC 1 cut(s) 2108
Sse8387I CCTGCAGG 1 cut(s) 2422
SsiI CCGC 6 cut(s) 878, 1049, 2032, 2364, 2413, 2454
SspMI CTAG 8 cut(s) 428, 555, 1056, 1328, 1431, 1518, 2304, 2436
StyD4I CCNGG 9 cut(s) 252, 480, 803, 918, 1074, 2094, 2105, 2106, 2458
StyI CCWWGG 2 cut(s) 649, 1117
TaaI ACNGT 8 cut(s) 211, 686, 1012, 1147, 1469, 1714, 2008, 2137
TaqI TCGA 7 cut(s) 337, 529, 1335, 1408, 1471, 2026, 2287
TatI WGTACW 2 cut(s) 1029, 1914
TauI GCSGC 3 cut(s) 880, 2415, 2456
TfiI GAWTC 6 cut(s) 354, 526, 531, 886, 1106, 1631
Tru1I TTAA 6 cut(s) 369, 1101, 1185, 1392, 1884, 2378
Tru9I TTAA 6 cut(s) 369, 1101, 1185, 1392, 1884, 2378
TscAI CASTG 4 cut(s) 552, 1690, 2013, 2434
TseFI GTSAC 4 cut(s) 430, 1006, 1520, 2309
Tsp45I GTSAC 4 cut(s) 430, 1006, 1520, 2309
TspDTI ATGAA 4 cut(s) 969, 1611, 1615, 2433
TspGWI ACGGA 1 cut(s) 1076
TspMI CCCGGG 1 cut(s) 2106
TspRI CASTG 4 cut(s) 552, 1690, 2013, 2434
Tth111I GACNNNGTC 1 cut(s) 2008
Vha464I CTTAAG 1 cut(s) 1100
VneI GTGCAC 1 cut(s) 1089
VpaK11BI GGWCC 3 cut(s) 407, 922, 1396
XapI RAATTY 5 cut(s) 339, 1208, 1267, 1485, 2353
XceI RCATGY 6 cut(s) 274, 1528, 1592, 1769, 1883, 1911
XmaI CCCGGG 1 cut(s) 2106
XmiI GTMKAC 1 cut(s) 1851
XspI CTAG 8 cut(s) 428, 555, 1056, 1328, 1431, 1518, 2304, 2436
ZrmI AGTACT 1 cut(s) 1031
Zsp2I ATGCAT 3 cut(s) 1552, 1590, 1606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.