Rh5DG138300

VWA / Hh protein intein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000360
Physical Location & Seq
Forward (+)
13914772 .. 13918938
4167 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG138300.1

Sequence Viewer

Length: 1887 bp
ATGGGTTCCGTGTTGAAGAAGCTGAAGAAGGCTCTCACTCCGAAGCTGCGCTGCGACTCCTCCTCCTCCACCGCAACCTCTCCGCCTCGGCCCTCCGAAACGACGTCGTCTGAGTTCTCCACAACATCTTCCAGCTTCTCCCGCGAGAAATGTTGCGCCATTTGCCTGAGTAACGTGAAAAGAGGGCAAGGTCAAGCCATCTTCACTGCTGAGTGTTCCCACTCTTTCCACTTCGACTGCATTGCCAAAAACGTCAAGCATGGAAATCTTTGCTGCCCTATCTGCAGGGCCAAATGGGATAGAATTAATGTACCGTTCCAAGCTCCCAATGATAGTGTGGATCCTCAGCGAAACCACTTGGGCGGTATCCATCCTCATCCTGAGCCCTTCTCTGACGATGAGCCTTTACTTACCACCTCTGCTACTCAGTCATCTGTCTCCCAGAGTGTGACAATTAAGACTCACACAGAGTCACCTGCGATCTCTGCTTCAGAATCACGCGCAAATTTTCCTGTGCTTGTCAGCATTTGTGCACCACCTCTTGAGGACCCTAATGGCCGCAGTCGTACACCCGTTGACCTTGTAACAGTTCTGGATGTAAGTGGCAGCATGGGTGAAACAAAGCTTTCCCTTGTCAAGAGTGCTGTCAAATTTCTCATCCAAAACTTGAGGCCTTCAGACCGGCTGTCTATAGTATCATTCTCAACAGCTGCTACAAGGGTGTTTCCTCTACGAAGAATGTCTGATGATGGCCGTGAGAGTGCCATCCTAGCTGTTGAATCACTTAGATCAAGTGGTGGAACTAGCATTGCGGAAGGACTCAAGAAAGGAACTCGAGTCCTTGAAGAACGAAGAGAAAGGAACCCAGTTGCTAGCATCATGCTCTTATCCGATGGCATCGATTCATACTATCCAGGTGGAAGCCAACTGGAGAAGCAATTGCCTGCCTCTATTTTTTCTAGCAAAACAGAACATGGAATTCCAGTCCACACATTTGGGTTTGGCAAGGACCATTGTCCAACTACTTTGCATACTATATCTGATGCATCAGGCGGAACCTTTTCCTTCATTGAATCAGTTGAGATGATACAAGATTCCTTCGCTATATGCATTGGTGGTCTTCTCAGTGTTGTGACTCAAGAATTGCGACTGACACTCAAGTCAGCAACACGTGGGGTGATGATTGTAGCAATACCATCTGGGAGATATTTCAGTGAGATTTCTGAAGAGGGCAAGCAAGGTGTTGTTACTGTTGGAAATATGTACGCAGAAGAGGAGAAGCAATTTCTGGTATACCTCTTTGTTCCGAAATCCTCAAGAAGTGCCACCAAAACATCATTGTTGGAGGTGTTATGTATGTATAAAGATCTAGCTGCAAATGAGTTGATTCAGGTTCCAGGTGAAAGAATAGAAATACTGAGACCTAAGGTTTGTTCCCCTGCTGAAAACGCAGTGTCTTTGGAGGTTGATCGGCAGTGCAACAGAGTATCAGTCGCTGAAGCTATTGCAGAGGCACAACGGATGGCTGAGATGGGAAATCTGAAGGGTGCAAAGGCGACTTTGGCTCAAGAAAGATCAACTCTCTTGTCATCACCAGCTGCCCAAGCCGGGGACAGCCTCATTACTTTGCTTGAAGCTCAGCTCACCGAAGTCATTCATAGAATGGCAACAATGGATTTGTATACACTGACCGGCCGTGCTTACGCTCTCTCAGAAATGAGCTCGCACTCATTGCAAAGAGCCGCAGCTACAGGTGACTCGACAACCTGGAGTCTCACCATGAGTGAAGGTGGAAATTCTGCCACCAGTAGTCGTGCGTTTGAAACACCTCATATGAGCAGAATGCTGAGGTTATCGCATAATCTTGGGACTAATCCTGCAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VWA VWA_3 Vwaint zf-RING_11

Protein Analysis

628

Amino Acids

67.69

Weight (kDa)

7.21

Isoelectric Point (pI)

52.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 51 - 96 3.1e-06 Ring finger domain
zf-RING_11 PF17123 52 - 81 4.8e-10 RING-like zinc finger
zf-C3HC4 PF00097 52 - 95 1.6e-06 Zinc finger, C3HC4 type (RING finger)
VWA_CoxE PF05762 184 - 307 1.3e-10 VWA domain containing CoxE-like protein
VWA PF00092 193 - 361 2.4e-22 von Willebrand factor type A domain
VWA_3 PF13768 193 - 356 1.3e-16 von Willebrand factor type A domain
VWA_2 PF13519 194 - 299 5.7e-18 von Willebrand factor type A domain
WAV3_C PF25243 499 - 555 9e-06 E3 ubiquitin-protein ligase WAV3-like, C-terminal domain
Vwaint PF14624 540 - 616 1.6e-09 VWA / Hh protein intein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000360)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11691 FvH4_3g11693 FvH4_3g11740 FvH4_3g11750 FvH4_3g11770 FvH4_3g11771 FvH4_3g11772 FvH4_3g11780
malus_domestica MD10G1239000.v1.1
prunus_persica Prupe.4G105400_v2.0.a1
pyrus_communis pycom10g20000
rosa_chinensis RchiOBHm_Chr5g0018981 RchiOBHm_Chr5g0018991 RchiOBHm_Chr5g0019101 RchiOBHm_Chr5g0019111 RchiOBHm_Chr5g0019121 RchiOBHm_Chr5g0019151 RchiOBHm_Chr5g0019161 RchiOBHm_Chr5g0019171 RchiOBHm_Chr5g0019181 RchiOBHm_Chr5g0019201
rosa_laevigata RLG00000032411 RLG00000032418 RLG00000032419 RLG00000032420 RLG00000032421 RLG00000032423 RLG00000032424 RLG00000032426
rosa_multiflora Rmu_co8475613.1_g000001 Rmu_co8503587.1_g000001 Rmu_sc0000563.1_g000023 Rmu_sc0000563.1_g000027 Rmu_sc0002142.1_g000001 Rmu_sc0002142.1_g000003 Rmu_sc0002142.1_g000004 Rmu_sc0002142.1_g000011 Rmu_sc0002142.1_g000022 Rmu_sc0002561.1_g000002 Rmu_sc0042836.1_g000001
rosa_roxburghii Rroxscaffold_1G00058790 Rroxscaffold_1G00058810 Rroxscaffold_1G00058820 Rroxscaffold_1G00058840 Rroxscaffold_1G00058850 Rroxscaffold_1G00058860 Rroxscaffold_1G00058880 Rroxscaffold_1G00058910 Rroxscaffold_1G00074090 Rroxscaffold_1G00074110 Rroxscaffold_1G00074120 Rroxscaffold_1G00074130 Rroxscaffold_1G00074150
rosa_rugosa Rorug05G0046800 Rorug05G0047200 Rorug05G0047300 Rorug05G0047400 Rorug05G0047600 Rorug05G0047600 Rorug05G0047800 Rorug05G0047900 Rorug05G0048000 Rorug05G0048100 Rorug05G0048100 Rorug05G0048200 Rorug05G0048300
rosa_samantha Rh4DG102800 Rh5AG138700 Rh5AG138900 Rh5AG139000 Rh5AG139100 Rh5AG139200 Rh5AG139400 Rh5AG139500 Rh5AG139600 Rh5BG137400 Rh5BG137600 Rh5BG137800 Rh5BG138100 Rh5BG138200 Rh5BG138300 Rh5BG138500 Rh5CG148800 Rh5CG148900 Rh5CG149200 Rh5CG149300 Rh5CG149400 Rh5CG149600 Rh5CG149700 Rh5CG150000 Rh5DG138200 Rh5DG138300 Rh5DG138500 Rh5DG138600 Rh5DG138700 Rh5DG138900 Rh5DG139000 Rh5DG139200
rosa_wichuraiana Rw5G012290 Rw5G012330 Rw5G012340 Rw5G012350 Rw5G012370 Rw5G012380 Rw5G012390 Rw5G012410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 484
AasI GACNNNNNNGTC 1 cut(s) 1159
AatII GACGTC 1 cut(s) 107
Acc36I ACCTGC 1 cut(s) 484
AccI GTMKAC 2 cut(s) 1293, 1682
AccII CGCG 2 cut(s) 144, 501
AciI CCGC 8 cut(s) 72, 83, 142, 363, 559, 812, 1053, 1743
AclWI GGATC 2 cut(s) 335, 348
AcoI YGGCCR 3 cut(s) 556, 751, 1693
AcsI RAATTY 4 cut(s) 505, 650, 978, 1795
AcuI CTGAAG 6 cut(s) 44, 474, 660, 1245, 1518, 1562
AcvI CACGTG 1 cut(s) 1172
AcyI GRCGYC 1 cut(s) 104
AfaI GTAC 3 cut(s) 312, 568, 1265
AfiI CCNNNNNNNGG 2 cut(s) 1608, 1609
AflIII ACRYGT 1 cut(s) 1169
AgsI TTSAA 6 cut(s) 16, 779, 845, 1073, 1634, 1823
AhdI GACNNNNNGTC 1 cut(s) 685
AjnI CCWGG 3 cut(s) 913, 1396, 1766
Alw21I GWGCWC 2 cut(s) 535, 1724
Alw26I GTCTC 3 cut(s) 442, 1414, 1778
Alw44I GTGCAC 1 cut(s) 531
AlwI GGATC 2 cut(s) 335, 348
AlwNI CAGNNNCTG 1 cut(s) 1496
Ama87I CYCGRG 1 cut(s) 834
AoxI GGCC 6 cut(s) 89, 288, 556, 671, 751, 1693
ApaLI GTGCAC 1 cut(s) 531
ApeKI GCWGC 9 cut(s) 46, 51, 273, 606, 710, 1373, 1598, 1745, 1880
ApoI RAATTY 4 cut(s) 505, 650, 978, 1795
ArsI GACNNNNNNTTYG 2 cut(s) 1413, 1445
AseI ATTAAT 1 cut(s) 306
Asp700I GAANNNNTTC 2 cut(s) 1060, 1653
AspLEI GCGC 3 cut(s) 51, 158, 503
AspS9I GGNCC 4 cut(s) 90, 288, 547, 1009
AsuC2I CCSGG 1 cut(s) 1609
AsuHPI GGTGA 8 cut(s) 465, 626, 1189, 1412, 1584, 1636, 1766, 1768
AsuNHI GCTAGC 1 cut(s) 872
AvaI CYCGRG 1 cut(s) 834
AvaII GGWCC 2 cut(s) 547, 1009
AxyI CCTNAGG 1 cut(s) 1425
BaeGI GKGCMC 1 cut(s) 535
BamHI GGATCC 1 cut(s) 340
BanII GRGCYC 2 cut(s) 387, 1724
BbrPI CACGTG 1 cut(s) 1172
BbsI GAAGAC 1 cut(s) 1112
Bbv12I GWGCWC 2 cut(s) 535, 1724
BbvCI CCTCAGC 2 cut(s) 345, 1847
BbvI GCAGC 8 cut(s) 33, 38, 260, 618, 697, 1360, 1585, 1757
BccI CCATC 8 cut(s) 206, 378, 743, 773, 887, 1204, 1516, 1525
BceAI ACGGC 2 cut(s) 738, 1680
BcgI CGANNNNNNTGC 2 cut(s) 224, 258
BciT130I CCWGG 3 cut(s) 915, 1398, 1768
BciVI GTATCC 1 cut(s) 377
BcnI CCSGG 1 cut(s) 1609
BcoDI GTCTC 3 cut(s) 442, 1414, 1778
BfaI CTAG 5 cut(s) 770, 804, 873, 960, 1370
BfmI CTRYAG 4 cut(s) 283, 690, 1749, 1878
BfuAI ACCTGC 1 cut(s) 484
BfuI GTATCC 1 cut(s) 377
BglII AGATCT 1 cut(s) 1366
BlpI GCTNAGC 1 cut(s) 1638
Bme1390I CCNGG 4 cut(s) 915, 1398, 1609, 1768
Bme18I GGWCC 2 cut(s) 547, 1009
BmeRI GACNNNNNGTC 1 cut(s) 685
BmeT110I CYCGRG 1 cut(s) 834
BmgT120I GGNCC 4 cut(s) 90, 288, 547, 1009
BmiI GGNNCC 6 cut(s) 7, 342, 549, 863, 1057, 1395
BmrFI CCNGG 4 cut(s) 915, 1398, 1609, 1768
BmrI ACTGGG 1 cut(s) 860
BmsI GCATC 4 cut(s) 885, 906, 1033, 1055
BmtI GCTAGC 1 cut(s) 876
BmuI ACTGGG 1 cut(s) 860
BoxI GACNNNNGTC 1 cut(s) 1014
BpiI GAAGAC 1 cut(s) 1112
BplI GAGNNNNNCTC 2 cut(s) 374, 406
BpmI CTGGAG 2 cut(s) 950, 1789
Bpu10I CCTNAGC 3 cut(s) 345, 381, 1847
Bpu1102I GCTNAGC 1 cut(s) 1638
BpuEI CTTGAG 7 cut(s) 563, 688, 806, 1122, 1142, 1300, 1551
BpuMI CCSGG 1 cut(s) 1609
Bsa29I ATCGAT 1 cut(s) 900
BsaAI YACGTR 1 cut(s) 1172
BsaHI GRCGYC 1 cut(s) 104
BsaI GGTCTC 1 cut(s) 1414
BsaJI CCNNGG 2 cut(s) 86, 1608
BsaXI ACNNNNNCTCC 2 cut(s) 47, 77
Bsc4I CCNNNNNNNGG 2 cut(s) 1608, 1609
Bse118I RCCGGY 2 cut(s) 681, 1691
Bse1I ACTGG 4 cut(s) 866, 933, 983, 1806
Bse21I CCTNAGG 1 cut(s) 1425
Bse3DI GCAATG 3 cut(s) 240, 807, 1730
BseBI CCWGG 3 cut(s) 915, 1398, 1768
BseCI ATCGAT 1 cut(s) 900
BseDI CCNNGG 2 cut(s) 86, 1608
BseGI GGATG 6 cut(s) 370, 376, 601, 657, 765, 1527
BseLI CCNNNNNNNGG 2 cut(s) 1608, 1609
BseMI GCAATG 3 cut(s) 240, 807, 1730
BseNI ACTGG 4 cut(s) 866, 933, 983, 1806
BseRI GAGGAG 4 cut(s) 49, 52, 55, 1289
BseSI GKGCMC 1 cut(s) 535
BseX3I CGGCCG 1 cut(s) 1693
BseXI GCAGC 8 cut(s) 33, 38, 260, 618, 697, 1360, 1585, 1757
Bsh1236I CGCG 2 cut(s) 144, 501
Bsh1285I CGRYCG 1 cut(s) 1696
BshFI GGCC 6 cut(s) 91, 290, 558, 673, 753, 1695
BshVI ATCGAT 1 cut(s) 900
BsiEI CGRYCG 1 cut(s) 1696
BsiHKAI GWGCWC 2 cut(s) 535, 1724
BsiHKCI CYCGRG 1 cut(s) 834
BsiSI CCGG 3 cut(s) 682, 1608, 1692
BslFI GGGAC 2 cut(s) 1625, 1882
BslI CCNNNNNNNGG 2 cut(s) 1608, 1609
BsmAI GTCTC 3 cut(s) 442, 1414, 1778
BsmFI GGGAC 2 cut(s) 1625, 1882
BsmI GAATGC 1 cut(s) 1848
BsnI GGCC 6 cut(s) 91, 290, 558, 673, 753, 1695
Bso31I GGTCTC 1 cut(s) 1414
BsoBI CYCGRG 1 cut(s) 834
Bsp1286I GDGCHC 3 cut(s) 387, 535, 1724
Bsp143I GATC 6 cut(s) 340, 480, 788, 1366, 1468, 1574
Bsp1720I GCTNAGC 1 cut(s) 1638
BspACI CCGC 8 cut(s) 72, 83, 142, 363, 559, 812, 1053, 1743
BspANI GGCC 6 cut(s) 91, 290, 558, 673, 753, 1695
BspDI ATCGAT 1 cut(s) 900
BspFNI CGCG 2 cut(s) 144, 501
BspLI GGNNCC 6 cut(s) 7, 342, 549, 863, 1057, 1395
BspMAI CTGCAG 2 cut(s) 287, 1882
BspMI ACCTGC 1 cut(s) 484
BspOI GCTAGC 1 cut(s) 876
BspPI GGATC 2 cut(s) 335, 348
BspTNI GGTCTC 1 cut(s) 1414
BsrDI GCAATG 3 cut(s) 240, 807, 1730
BsrFI RCCGGY 2 cut(s) 681, 1691
BsrI ACTGG 4 cut(s) 866, 933, 983, 1806
BssAI RCCGGY 2 cut(s) 681, 1691
BssECI CCNNGG 2 cut(s) 86, 1608
BssMI GATC 6 cut(s) 340, 480, 788, 1366, 1468, 1574
BssNAI GTATAC 2 cut(s) 1294, 1683
BssNI GRCGYC 1 cut(s) 104
Bst1107I GTATAC 2 cut(s) 1294, 1683
Bst2UI CCWGG 3 cut(s) 915, 1398, 1768
Bst4CI ACNGT 3 cut(s) 315, 589, 1252
Bst6I CTCTTC 3 cut(s) 847, 1221, 1266
BstACI GRCGYC 1 cut(s) 104
BstAPI GCANNNNNTGC 1 cut(s) 1732
BstBAI YACGTR 1 cut(s) 1172
BstC8I GCNNGC 4 cut(s) 874, 945, 1235, 1724
BstF5I GGATG 6 cut(s) 370, 376, 601, 657, 765, 1527
BstFNI CGCG 2 cut(s) 144, 501
BstHHI GCGC 3 cut(s) 51, 158, 503
BstKTI GATC 6 cut(s) 343, 483, 791, 1369, 1471, 1577
BstMAI GTCTC 3 cut(s) 442, 1414, 1778
BstMBI GATC 6 cut(s) 340, 480, 788, 1366, 1468, 1574
BstMCI CGRYCG 1 cut(s) 1696
BstMWI GCNNNNNNNGC 9 cut(s) 141, 162, 282, 485, 770, 1448, 1562, 1604, 1732
BstNI CCWGG 3 cut(s) 915, 1398, 1768
BstPAI GACNNNNGTC 1 cut(s) 1014
BstSCI CCNGG 4 cut(s) 913, 1396, 1607, 1766
BstSFI CTRYAG 4 cut(s) 283, 690, 1749, 1878
BstSLI GKGCMC 1 cut(s) 535
BstUI CGCG 2 cut(s) 144, 501
BstV1I GCAGC 8 cut(s) 33, 38, 260, 618, 697, 1360, 1585, 1757
BstV2I GAAGAC 1 cut(s) 1112
BstX2I RGATCY 2 cut(s) 340, 1366
BstXI CCANNNNNNTGG 1 cut(s) 995
BstYI RGATCY 2 cut(s) 340, 1366
BstZ17I GTATAC 2 cut(s) 1294, 1683
BstZI CGGCCG 1 cut(s) 1693
Bsu15I ATCGAT 1 cut(s) 900
Bsu36I CCTNAGG 1 cut(s) 1425
BsuI GTATCC 1 cut(s) 377
BsuRI GGCC 6 cut(s) 91, 290, 558, 673, 753, 1695
BsuTUI ATCGAT 1 cut(s) 900
BtsCI GGATG 6 cut(s) 370, 376, 601, 657, 765, 1527
BtsI GCAGTG 3 cut(s) 204, 1458, 1481
BtsIMutI CAGTG 6 cut(s) 204, 1132, 1219, 1458, 1481, 1685
BveI ACCTGC 1 cut(s) 484
Cac8I GCNNGC 4 cut(s) 874, 945, 1235, 1724
CaiI CAGNNNCTG 1 cut(s) 1496
CfoI GCGC 3 cut(s) 51, 158, 503
Cfr10I RCCGGY 2 cut(s) 681, 1691
Cfr13I GGNCC 4 cut(s) 90, 288, 547, 1009
ClaI ATCGAT 1 cut(s) 900
Csp6I GTAC 3 cut(s) 311, 567, 1264
CviAII CATG 6 cut(s) 260, 610, 880, 974, 1780, 1884
CviQI GTAC 3 cut(s) 311, 567, 1264
DpnI GATC 6 cut(s) 342, 482, 790, 1368, 1470, 1576
DpnII GATC 6 cut(s) 340, 480, 788, 1366, 1468, 1574
DrdI GACNNNNNNGTC 1 cut(s) 1159
DriI GACNNNNNGTC 1 cut(s) 685
DseDI GACNNNNNNGTC 1 cut(s) 1159
EaeI YGGCCR 3 cut(s) 556, 751, 1693
EagI CGGCCG 1 cut(s) 1693
Eam1104I CTCTTC 3 cut(s) 847, 1221, 1266
Eam1105I GACNNNNNGTC 1 cut(s) 685
EarI CTCTTC 3 cut(s) 847, 1221, 1266
EciI GGCGGA 2 cut(s) 72, 1068
Ecl136II GAGCTC 1 cut(s) 1722
EclXI CGGCCG 1 cut(s) 1693
Eco147I AGGCCT 1 cut(s) 673
Eco24I GRGCYC 2 cut(s) 387, 1724
Eco31I GGTCTC 1 cut(s) 1414
Eco47I GGWCC 2 cut(s) 547, 1009
Eco52I CGGCCG 1 cut(s) 1693
Eco53kI GAGCTC 1 cut(s) 1722
Eco57I CTGAAG 6 cut(s) 44, 474, 660, 1245, 1518, 1562
Eco72I CACGTG 1 cut(s) 1172
Eco81I CCTNAGG 1 cut(s) 1425
Eco88I CYCGRG 1 cut(s) 834
EcoICRI GAGCTC 1 cut(s) 1722
EcoO109I RGGNCCY 1 cut(s) 547
EcoRI GAATTC 1 cut(s) 978
EcoRII CCWGG 3 cut(s) 913, 1396, 1766
EcoT22I ATGCAT 2 cut(s) 1048, 1112
EcoT38I GRGCYC 2 cut(s) 387, 1724
FaeI CATG 6 cut(s) 263, 613, 883, 977, 1783, 1887
FaqI GGGAC 2 cut(s) 1625, 1882
FatI CATG 6 cut(s) 259, 609, 879, 973, 1779, 1883
FauI CCCGC 1 cut(s) 149
FauNDI CATATG 1 cut(s) 1833
FblI GTMKAC 2 cut(s) 1293, 1682
FokI GGATG 6 cut(s) 357, 363, 608, 644, 752, 1534
FriOI GRGCYC 2 cut(s) 387, 1724
FspBI CTAG 5 cut(s) 770, 804, 873, 960, 1370
GlaI GCGC 3 cut(s) 50, 157, 502
GsuI CTGGAG 2 cut(s) 950, 1789
HaeIII GGCC 6 cut(s) 91, 290, 558, 673, 753, 1695
HapII CCGG 3 cut(s) 682, 1608, 1692
HhaI GCGC 3 cut(s) 51, 158, 503
Hin1I GRCGYC 1 cut(s) 104
Hin1II CATG 6 cut(s) 263, 613, 883, 977, 1783, 1887
Hin6I GCGC 3 cut(s) 49, 156, 501
HinP1I GCGC 3 cut(s) 49, 156, 501
HincII GTYRAC 1 cut(s) 577
HindII GTYRAC 1 cut(s) 577
HindIII AAGCTT 1 cut(s) 623
HpaII CCGG 3 cut(s) 682, 1608, 1692
HphI GGTGA 8 cut(s) 465, 626, 1189, 1412, 1584, 1636, 1766, 1768
Hpy166II GTNNAC 6 cut(s) 533, 569, 577, 988, 1294, 1683
Hpy188III TCNNGA 8 cut(s) 380, 542, 593, 637, 823, 1139, 1317, 1568
Hpy8I GTNNAC 6 cut(s) 533, 569, 577, 988, 1294, 1683
Hpy99I CGWCG 2 cut(s) 106, 109
HpyAV CCTTC 8 cut(s) 22, 397, 684, 809, 1075, 1108, 1537, 1781
HpyCH4III ACNGT 3 cut(s) 315, 589, 1252
HpyCH4IV ACGT 4 cut(s) 104, 174, 252, 1171
HpyF10VI GCNNNNNNNGC 9 cut(s) 141, 162, 282, 485, 770, 1448, 1562, 1604, 1732
HpySE526I ACGT 4 cut(s) 104, 174, 252, 1171
Hsp92I GRCGYC 1 cut(s) 104
Hsp92II CATG 6 cut(s) 263, 613, 883, 977, 1783, 1887
HspAI GCGC 3 cut(s) 49, 156, 501
Kzo9I GATC 6 cut(s) 340, 480, 788, 1366, 1468, 1574
LmnI GCTCC 1 cut(s) 328
Lsp1109I GCAGC 8 cut(s) 33, 38, 260, 618, 697, 1360, 1585, 1757
LweI GCATC 4 cut(s) 885, 906, 1033, 1055
MaeI CTAG 5 cut(s) 770, 804, 873, 960, 1370
MaeII ACGT 4 cut(s) 104, 174, 252, 1171
MaeIII GTNAC 7 cut(s) 170, 448, 471, 583, 1132, 1246, 1754
MalI GATC 6 cut(s) 342, 482, 790, 1368, 1470, 1576
MboI GATC 6 cut(s) 340, 480, 788, 1366, 1468, 1574
MfeI CAATTG 1 cut(s) 938
MflI RGATCY 2 cut(s) 340, 1366
MhlI GDGCHC 3 cut(s) 387, 535, 1724
MluCI AATT 9 cut(s) 303, 453, 505, 650, 938, 978, 1142, 1283, 1795
MlyI GAGTC 8 cut(s) 50, 454, 479, 813, 846, 1129, 1751, 1780
MmeI TCCRAC 3 cut(s) 1043, 1234, 1323
Mph1103I ATGCAT 2 cut(s) 1048, 1112
MroXI GAANNNNTTC 2 cut(s) 1060, 1653
MseI TTAA 2 cut(s) 306, 456
MspA1I CMGCKG 2 cut(s) 710, 1598
MspI CCGG 3 cut(s) 682, 1608, 1692
MspR9I CCNGG 4 cut(s) 915, 1398, 1609, 1768
MunI CAATTG 1 cut(s) 938
Mva1269I GAATGC 1 cut(s) 1848
MvaI CCWGG 3 cut(s) 915, 1398, 1768
MvnI CGCG 2 cut(s) 144, 501
MwoI GCNNNNNNNGC 9 cut(s) 141, 162, 282, 485, 770, 1448, 1562, 1604, 1732
NciI CCSGG 1 cut(s) 1609
NdeI CATATG 1 cut(s) 1833
NdeII GATC 6 cut(s) 340, 480, 788, 1366, 1468, 1574
NheI GCTAGC 1 cut(s) 872
NlaIII CATG 6 cut(s) 263, 613, 883, 977, 1783, 1887
NlaIV GGNNCC 6 cut(s) 7, 342, 549, 863, 1057, 1395
NmeAIII GCCGAG 1 cut(s) 67
NmuCI GTSAC 4 cut(s) 448, 471, 1132, 1754
NsiI ATGCAT 2 cut(s) 1048, 1112
PaeR7I CTCGAG 1 cut(s) 834
PaqCI CACCTGC 1 cut(s) 484
PceI AGGCCT 1 cut(s) 673
PctI GAATGC 1 cut(s) 1848
PdmI GAANNNNTTC 2 cut(s) 1060, 1653
PfeI GAWTC 6 cut(s) 494, 779, 902, 1073, 1094, 1387
PflFI GACNNNGTC 1 cut(s) 106
PleI GAGTC 8 cut(s) 50, 454, 478, 813, 845, 1129, 1751, 1779
PmaCI CACGTG 1 cut(s) 1172
PmlI CACGTG 1 cut(s) 1172
PpsI GAGTC 8 cut(s) 50, 454, 478, 813, 845, 1129, 1751, 1779
Ppu21I YACGTR 1 cut(s) 1172
PpuMI RGGWCCY 1 cut(s) 547
PshAI GACNNNNGTC 1 cut(s) 1014
PshBI ATTAAT 1 cut(s) 306
Psp124BI GAGCTC 1 cut(s) 1724
Psp5II RGGWCCY 1 cut(s) 547
Psp6I CCWGG 3 cut(s) 913, 1396, 1766
PspCI CACGTG 1 cut(s) 1172
PspGI CCWGG 3 cut(s) 913, 1396, 1766
PspN4I GGNNCC 6 cut(s) 7, 342, 549, 863, 1057, 1395
PspPI GGNCC 4 cut(s) 90, 288, 547, 1009
PspPPI RGGWCCY 1 cut(s) 547
PspXI VCTCGAGB 1 cut(s) 834
PstI CTGCAG 2 cut(s) 287, 1882
PstNI CAGNNNCTG 1 cut(s) 1496
PsuI RGATCY 2 cut(s) 340, 1366
PsyI GACNNNGTC 1 cut(s) 106
PvuII CAGCTG 2 cut(s) 710, 1598
RsaI GTAC 3 cut(s) 312, 568, 1265
RsaNI GTAC 3 cut(s) 311, 567, 1264
SacI GAGCTC 1 cut(s) 1724
SaqAI TTAA 2 cut(s) 306, 456
Sau3AI GATC 6 cut(s) 340, 480, 788, 1366, 1468, 1574
Sau96I GGNCC 4 cut(s) 90, 288, 547, 1009
SchI GAGTC 8 cut(s) 50, 454, 479, 813, 846, 1129, 1751, 1780
ScrFI CCNGG 4 cut(s) 915, 1398, 1609, 1768
SduI GDGCHC 3 cut(s) 387, 535, 1724
SfaNI GCATC 4 cut(s) 885, 906, 1033, 1055
SfcI CTRYAG 4 cut(s) 283, 690, 1749, 1878
Sfr274I CTCGAG 1 cut(s) 834
SinI GGWCC 2 cut(s) 547, 1009
SlaI CTCGAG 1 cut(s) 834
SmlI CTYRAG 8 cut(s) 542, 667, 821, 834, 1137, 1157, 1315, 1566
SmoI CTYRAG 8 cut(s) 542, 667, 821, 834, 1137, 1157, 1315, 1566
Sse9I AATT 9 cut(s) 303, 453, 505, 650, 938, 978, 1142, 1283, 1795
SseBI AGGCCT 1 cut(s) 673
SsiI CCGC 8 cut(s) 72, 83, 142, 363, 559, 812, 1053, 1743
SspMI CTAG 5 cut(s) 770, 804, 873, 960, 1370
SstI GAGCTC 1 cut(s) 1724
StuI AGGCCT 1 cut(s) 673
StyD4I CCNGG 4 cut(s) 913, 1396, 1607, 1766
TaaI ACNGT 3 cut(s) 315, 589, 1252
TaiI ACGT 4 cut(s) 107, 177, 255, 1174
TaqI TCGA 4 cut(s) 234, 835, 900, 1760
TasI AATT 9 cut(s) 303, 453, 505, 650, 938, 978, 1142, 1283, 1795
TauI GCSGC 2 cut(s) 561, 1745
TfiI GAWTC 6 cut(s) 494, 779, 902, 1073, 1094, 1387
Tru1I TTAA 2 cut(s) 306, 456
Tru9I TTAA 2 cut(s) 306, 456
TscAI CASTG 6 cut(s) 211, 1132, 1219, 1458, 1481, 1692
TseFI GTSAC 4 cut(s) 448, 471, 1132, 1754
TseI GCWGC 9 cut(s) 46, 51, 273, 606, 710, 1373, 1598, 1745, 1880
Tsp45I GTSAC 4 cut(s) 448, 471, 1132, 1754
TspDTI ATGAA 3 cut(s) 894, 1057, 1646
TspGWI ACGGA 1 cut(s) 1534
TspRI CASTG 6 cut(s) 211, 1132, 1219, 1458, 1481, 1692
Tth111I GACNNNGTC 1 cut(s) 106
VneI GTGCAC 1 cut(s) 531
VpaK11BI GGWCC 2 cut(s) 547, 1009
VspI ATTAAT 1 cut(s) 306
XapI RAATTY 4 cut(s) 505, 650, 978, 1795
XcmI CCANNNNNNNNNTGG 1 cut(s) 334
XhoI CTCGAG 1 cut(s) 834
XmiI GTMKAC 2 cut(s) 1293, 1682
XmnI GAANNNNTTC 2 cut(s) 1060, 1653
XspI CTAG 5 cut(s) 770, 804, 873, 960, 1370
ZraI GACGTC 1 cut(s) 105
Zsp2I ATGCAT 2 cut(s) 1048, 1112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.