RH5BG138300

VWA / Hh protein intein-like

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_samantha
Unknown
OG0000360
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG138300.1

Sequence Viewer

Length: 1932 bp
ATGAACAATTGCGGAATTTGCAGGAGTAGCCTGACAAGAGGGCTAAGCGAAGCCATCTTCACTGCCGAGTGCTCGCACGTTTTCCACTACCATTGCATTCTCAAAAATGTCCAGCATGGAAATCTTTGCTGCCCTGTCTGCCAAGCAAAGTGGAATAAGGATAATGTACCTTTCCAGGTTCCCCCTCCTCAGAACAACAACTTGGGCACAATCCCTTGTCACTTCACATTCCCATCACAGCAGCCACCCCATTGGTATTATCCTCAGTACCCATCCATGCCAAAACCCGAGTACCCTCCTGAGCCACCTACCTTTTGTGACGATGAACCTCTCCCATCGTGCACCTCATCCATTCAATCCTCTGCCCCTCAGAATATAACAGTCAAGACTCATACAGAGTCCTCTGCTGTCTCTTCTGCAGAATCAATCCCAAATTTTCCCGTCCTTGTCAGCATTCTTGCACCGCCCATGCAGGATTCTGAGGGACATGGTCGTACACCCATTGACCTTGTAACAGTTCTAGACGTAAGTGGCAGCATGGAAGGCACAAAGCTTTCCCTTCTCAAGCAAGCTGTCACATTTGTCATAGAAAACTTGGGGCCTTCGGATAGACTTTCTATAGTTTCGTTCTCATCAAGTTCTAGAAGAGTCTTTCCTCTCCAGAGAATGTCTGTTGATGGCCGTGAAAGTGCTGTCCTAGCCATTGAGTCTCTTAGAGCAGGTGGTGCAACTAACATTGCAGAAGGACTGAAGAAAGGAACTAAGGTCCTTGAAGATCGTAGGGAAAGAAACCCAGTTTCCAGTATCATCCTCTTATCTGATGGCATAGATACATACAATAGAACTGCAAACCGACTGTTGAACCAGTTGCCTGATTCAATTCGTTCTAGCAACATGCAACATGAAATCCCAGTCCACACATTTGGATTTGGCTATGACCATGATCCCAACATTATGCATGCTATATCTGATCTGTCAGGCGGCACCTTTTCGTTCATTGAATCAGTTGGAATGATACAAGACTCCTTTGCTCTGTGTATTGGTGGTCTTCTCAGTGTTGTGGCTCAGGAAGTTCGCCTAACAGTAAGGTCAGCATCGCCTGGGGTGAAGATTTTAGCAATACCATCAGGGAGACACGTGAATGAGATTTCTGATGAGGGTCAACAAGGAGTTGTTCACGTTGGAAATATGTATGCAGAAGAGGAGAAACAATTTCTGGTCTACCTCTTAGTTCCAGAATCCTCAGGTCCGTATACTAAGATGTCATTGTTAGATGTGTTATGCATGTACAAAGATCTAGCTTCCAATGAACTCATCCAGGTGCAAGGTGCGGAAATAGAGATACTGAGACCCGAGGTTTGTTCCCCTGCAGAAAAGGCAGTGTCTTTGGAGGTTGATCGACAGCGGAACAGAATTGTGGTTGCTGAAACTATTGCAGAGGCACAAAGGTTGGCTGAGATGGGAAACCTGGAAGGTGCACGGGCACTTTTGGCTGAACGAAGAGCAACTGTCTTAGCATCAGCAGCAGCTCAAGCTGGGGATGGTCTCAGTAATTTGTTTGAAACTGAGCTTAGAGAAGTCATGGAGAGAATGGCATCTATGGACTTGTACGCTAACTCAGGGCGTGCTTACGCTCTCGCAGGGATGAGTTCTCATTCTCGACAGAGGGCTTCAGCTAGGGGTAACACAATAACCTCATGTCTCTTTGGTGCCTCCAACCGTGTGAATTTCTCTAGCGGTGTCAATGCCTTAATGCAGCAGTCGGGTGCCTCTGCTTGTTTTGGCGCCCCTGCAGGTTTCGGTGGTGCTAGTTTTTCATCCGGTGCCGCCCCAGGTGGAAGTCAATGCTCTTCTGCAGTTGGTGCTTTTGAAACACCTGCTATGGTTAGAATGGTAGAAAAATCACAGAAGAGCAATCAATCTGGGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VWA Vwaint zf-RING_11 zf-RING_2

Protein Analysis

643

Amino Acids

69.15

Weight (kDa)

5.73

Isoelectric Point (pI)

60.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 2 - 48 7.9e-06 Ring finger domain
zf-RING_11 PF17123 3 - 33 1.3e-07 RING-like zinc finger
zf-C3HC4_2 PF13923 4 - 47 8.2e-06 Zinc finger, C3HC4 type (RING finger)
VWA_CoxE PF05762 165 - 290 3.5e-07 VWA domain containing CoxE-like protein
VWA PF00092 169 - 334 2.6e-21 von Willebrand factor type A domain
VWA_3 PF13768 169 - 332 1.1e-18 von Willebrand factor type A domain
VWA_2 PF13519 170 - 275 1.5e-18 von Willebrand factor type A domain
WAV3_C PF25243 465 - 531 7.2e-06 E3 ubiquitin-protein ligase WAV3-like, C-terminal domain
Vwaint PF14624 521 - 623 1.3e-10 VWA / Hh protein intein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000360)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11691 FvH4_3g11693 FvH4_3g11740 FvH4_3g11750 FvH4_3g11770 FvH4_3g11771 FvH4_3g11772 FvH4_3g11780
malus_domestica MD10G1239000.v1.1
prunus_persica Prupe.4G105400_v2.0.a1
pyrus_communis pycom10g20000
rosa_chinensis RchiOBHm_Chr5g0018981 RchiOBHm_Chr5g0018991 RchiOBHm_Chr5g0019101 RchiOBHm_Chr5g0019111 RchiOBHm_Chr5g0019121 RchiOBHm_Chr5g0019151 RchiOBHm_Chr5g0019161 RchiOBHm_Chr5g0019171 RchiOBHm_Chr5g0019181 RchiOBHm_Chr5g0019201
rosa_laevigata RLG00000032411 RLG00000032418 RLG00000032419 RLG00000032420 RLG00000032421 RLG00000032423 RLG00000032424 RLG00000032426
rosa_multiflora Rmu_co8475613.1_g000001 Rmu_co8503587.1_g000001 Rmu_sc0000563.1_g000023 Rmu_sc0000563.1_g000027 Rmu_sc0002142.1_g000001 Rmu_sc0002142.1_g000003 Rmu_sc0002142.1_g000004 Rmu_sc0002142.1_g000011 Rmu_sc0002142.1_g000022 Rmu_sc0002561.1_g000002 Rmu_sc0042836.1_g000001
rosa_roxburghii Rroxscaffold_1G00058790 Rroxscaffold_1G00058810 Rroxscaffold_1G00058820 Rroxscaffold_1G00058840 Rroxscaffold_1G00058850 Rroxscaffold_1G00058860 Rroxscaffold_1G00058880 Rroxscaffold_1G00058910 Rroxscaffold_1G00074090 Rroxscaffold_1G00074110 Rroxscaffold_1G00074120 Rroxscaffold_1G00074130 Rroxscaffold_1G00074150
rosa_rugosa Rorug05G0046800 Rorug05G0047200 Rorug05G0047300 Rorug05G0047400 Rorug05G0047600 Rorug05G0047600 Rorug05G0047800 Rorug05G0047900 Rorug05G0048000 Rorug05G0048100 Rorug05G0048100 Rorug05G0048200 Rorug05G0048300
rosa_samantha Rh4DG102800 Rh5AG138700 Rh5AG138900 Rh5AG139000 Rh5AG139100 Rh5AG139200 Rh5AG139400 Rh5AG139500 Rh5AG139600 Rh5BG137400 Rh5BG137600 Rh5BG137800 Rh5BG138100 Rh5BG138200 Rh5BG138300 Rh5BG138500 Rh5CG148800 Rh5CG148900 Rh5CG149200 Rh5CG149300 Rh5CG149400 Rh5CG149600 Rh5CG149700 Rh5CG150000 Rh5DG138200 Rh5DG138300 Rh5DG138500 Rh5DG138600 Rh5DG138700 Rh5DG138900 Rh5DG139000 Rh5DG139200
rosa_wichuraiana Rw5G012290 Rw5G012330 Rw5G012340 Rw5G012350 Rw5G012370 Rw5G012380 Rw5G012390 Rw5G012410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 710, 1885
Acc36I ACCTGC 3 cut(s) 710, 1784, 1885
AccB1I GGYRCC 5 cut(s) 983, 1709, 1766, 1784, 1823
AccI GTMKAC 2 cut(s) 1221, 1253
AciI CCGC 7 cut(s) 12, 464, 981, 1331, 1405, 1737, 1827
AclWI GGATC 1 cut(s) 938
AcoI YGGCCR 1 cut(s) 679
AcsI RAATTY 3 cut(s) 15, 433, 1726
AcuI CTGAAG 2 cut(s) 770, 1656
AcvI CACGTG 1 cut(s) 1138
AcyI GRCGYC 1 cut(s) 1785
AfaI GTAC 6 cut(s) 168, 269, 293, 496, 1289, 1610
AflIII ACRYGT 1 cut(s) 1135
AgsI TTSAA 7 cut(s) 356, 773, 862, 879, 1001, 1562, 1871
AjnI CCWGG 5 cut(s) 174, 1099, 1317, 1467, 1831
AjuI GAANNNNNNNTTGG 2 cut(s) 185, 217
AluBI AGCT 7 cut(s) 553, 572, 1301, 1529, 1535, 1570, 1676
AluI AGCT 7 cut(s) 553, 572, 1301, 1529, 1535, 1570, 1676
Alw21I GWGCWC 3 cut(s) 74, 344, 1480
Alw26I GTCTC 6 cut(s) 415, 714, 1126, 1342, 1550, 1706
Alw44I GTGCAC 2 cut(s) 340, 1476
AlwI GGATC 1 cut(s) 938
Ama87I CYCGRG 2 cut(s) 287, 1352
AoxI GGCC 2 cut(s) 599, 679
ApaLI GTGCAC 2 cut(s) 340, 1476
ApeKI GCWGC 6 cut(s) 129, 241, 534, 1523, 1526, 1756
ApoI RAATTY 3 cut(s) 15, 433, 1726
ArsI GACNNNNNNTTYG 2 cut(s) 1341, 1373
AspLEI GCGC 1 cut(s) 1787
AspS9I GGNCC 3 cut(s) 599, 766, 1247
AsuHPI GGTGA 1 cut(s) 1117
AvaI CYCGRG 2 cut(s) 287, 1352
AvaII GGWCC 2 cut(s) 766, 1247
AxyI CCTNAGG 1 cut(s) 1243
BaeGI GKGCMC 4 cut(s) 209, 344, 1480, 1486
BaeI ACNNNNGTAYC 2 cut(s) 275, 308
BanI GGYRCC 5 cut(s) 983, 1709, 1766, 1784, 1823
BbrPI CACGTG 1 cut(s) 1138
BbsI GAAGAC 1 cut(s) 1040
Bbv12I GWGCWC 3 cut(s) 74, 344, 1480
BbvI GCAGC 6 cut(s) 116, 253, 546, 1535, 1538, 1768
BccI CCATC 9 cut(s) 62, 241, 280, 343, 671, 815, 1132, 1453, 1535
BceAI ACGGC 1 cut(s) 666
BciT130I CCWGG 5 cut(s) 176, 1101, 1319, 1469, 1833
BcoDI GTCTC 6 cut(s) 415, 714, 1126, 1342, 1550, 1706
BfaI CTAG 8 cut(s) 521, 642, 698, 888, 1298, 1677, 1734, 1809
BfmI CTRYAG 5 cut(s) 417, 618, 1368, 1791, 1854
BfoI RGCGCY 1 cut(s) 1788
BfuAI ACCTGC 3 cut(s) 710, 1784, 1885
BglII AGATCT 1 cut(s) 1294
BisI GCNGC 8 cut(s) 130, 242, 535, 982, 1524, 1527, 1757, 1827
BlpI GCTNAGC 1 cut(s) 44
BlsI GCNGC 8 cut(s) 131, 243, 536, 983, 1525, 1528, 1758, 1828
Bme1390I CCNGG 5 cut(s) 176, 1101, 1319, 1469, 1833
Bme18I GGWCC 2 cut(s) 766, 1247
BmeT110I CYCGRG 2 cut(s) 287, 1352
BmgT120I GGNCC 3 cut(s) 599, 766, 1247
BmiI GGNNCC 7 cut(s) 180, 600, 985, 1711, 1768, 1786, 1825
BmrFI CCNGG 5 cut(s) 176, 1101, 1319, 1469, 1833
BmrI ACTGGG 2 cut(s) 788, 905
BmsI GCATC 3 cut(s) 1103, 1526, 1604
BmuI ACTGGG 2 cut(s) 788, 905
BpiI GAAGAC 1 cut(s) 1040
BpmI CTGGAG 1 cut(s) 644
Bpu10I CCTNAGC 2 cut(s) 300, 1065
Bpu1102I GCTNAGC 1 cut(s) 44
BpuEI CTTGAG 2 cut(s) 548, 1515
BsaAI YACGTR 1 cut(s) 1138
BsaHI GRCGYC 1 cut(s) 1785
BsaI GGTCTC 2 cut(s) 1342, 1550
BsaJI CCNNGG 3 cut(s) 1100, 1353, 1831
BsaWI WCCGGW 1 cut(s) 1820
Bse1I ACTGG 4 cut(s) 794, 801, 865, 911
Bse21I CCTNAGG 1 cut(s) 1243
Bse3DI GCAATG 2 cut(s) 91, 735
BseBI CCWGG 5 cut(s) 176, 1101, 1319, 1469, 1833
BseDI CCNNGG 3 cut(s) 1100, 1353, 1831
BseGI GGATG 7 cut(s) 272, 347, 807, 1314, 1546, 1650, 1817
BseMI GCAATG 2 cut(s) 91, 735
BseNI ACTGG 4 cut(s) 794, 801, 865, 911
BseRI GAGGAG 2 cut(s) 177, 1217
BseSI GKGCMC 4 cut(s) 209, 344, 1480, 1486
BseXI GCAGC 6 cut(s) 116, 253, 546, 1535, 1538, 1768
BseYI CCCAGC 1 cut(s) 1535
BshFI GGCC 2 cut(s) 601, 681
BshNI GGYRCC 5 cut(s) 983, 1709, 1766, 1784, 1823
BsiHKAI GWGCWC 3 cut(s) 74, 344, 1480
BsiHKCI CYCGRG 2 cut(s) 287, 1352
BsiSI CCGG 1 cut(s) 1821
BslFI GGGAC 1 cut(s) 498
BsmAI GTCTC 6 cut(s) 415, 714, 1126, 1342, 1550, 1706
BsmFI GGGAC 1 cut(s) 498
BsmI GAATGC 2 cut(s) 96, 453
BsnI GGCC 2 cut(s) 601, 681
Bso31I GGTCTC 2 cut(s) 1342, 1550
BsoBI CYCGRG 2 cut(s) 287, 1352
Bsp1286I GDGCHC 5 cut(s) 74, 209, 344, 1480, 1486
Bsp1407I TGTACA 1 cut(s) 1287
Bsp143I GATC 5 cut(s) 775, 943, 970, 1294, 1396
Bsp1720I GCTNAGC 1 cut(s) 44
BspACI CCGC 7 cut(s) 12, 464, 981, 1331, 1405, 1737, 1827
BspANI GGCC 2 cut(s) 601, 681
BspLI GGNNCC 7 cut(s) 180, 600, 985, 1711, 1768, 1786, 1825
BspMAI CTGCAG 4 cut(s) 421, 1372, 1795, 1858
BspMI ACCTGC 3 cut(s) 710, 1784, 1885
BspPI GGATC 1 cut(s) 938
BspQI GCTCTTC 3 cut(s) 1495, 1855, 1904
BspT107I GGYRCC 5 cut(s) 983, 1709, 1766, 1784, 1823
BspTNI GGTCTC 2 cut(s) 1342, 1550
BsrDI GCAATG 2 cut(s) 91, 735
BsrGI TGTACA 1 cut(s) 1287
BsrI ACTGG 4 cut(s) 794, 801, 865, 911
BssECI CCNNGG 3 cut(s) 1100, 1353, 1831
BssMI GATC 5 cut(s) 775, 943, 970, 1294, 1396
BssNAI GTATAC 1 cut(s) 1254
BssNI GRCGYC 1 cut(s) 1785
Bst1107I GTATAC 1 cut(s) 1254
Bst2UI CCWGG 5 cut(s) 176, 1101, 1319, 1469, 1833
Bst4CI ACNGT 6 cut(s) 382, 517, 858, 1084, 1510, 1721
Bst6I CTCTTC 6 cut(s) 418, 640, 1194, 1495, 1855, 1904
BstACI GRCGYC 1 cut(s) 1785
BstAPI GCANNNNNTGC 2 cut(s) 725, 1862
BstAUI TGTACA 1 cut(s) 1287
BstBAI YACGTR 1 cut(s) 1138
BstC8I GCNNGC 4 cut(s) 74, 570, 960, 1626
BstF5I GGATG 7 cut(s) 272, 347, 807, 1314, 1546, 1650, 1817
BstH2I RGCGCY 1 cut(s) 1788
BstHHI GCGC 1 cut(s) 1787
BstKTI GATC 5 cut(s) 778, 946, 973, 1297, 1399
BstMAI GTCTC 6 cut(s) 415, 714, 1126, 1342, 1550, 1706
BstMBI GATC 5 cut(s) 775, 943, 970, 1294, 1396
BstNI CCWGG 5 cut(s) 176, 1101, 1319, 1469, 1833
BstNSI RCATGY 3 cut(s) 898, 962, 1288
BstSCI CCNGG 5 cut(s) 174, 1099, 1317, 1467, 1831
BstSFI CTRYAG 5 cut(s) 417, 618, 1368, 1791, 1854
BstSLI GKGCMC 4 cut(s) 209, 344, 1480, 1486
BstV1I GCAGC 6 cut(s) 116, 253, 546, 1535, 1538, 1768
BstV2I GAAGAC 1 cut(s) 1040
BstX2I RGATCY 1 cut(s) 1294
BstXI CCANNNNNNTGG 2 cut(s) 252, 923
BstYI RGATCY 1 cut(s) 1294
BstZ17I GTATAC 1 cut(s) 1254
Bsu36I CCTNAGG 1 cut(s) 1243
BsuRI GGCC 2 cut(s) 601, 681
BtgZI GCGATG 1 cut(s) 1080
BtsCI GGATG 7 cut(s) 272, 347, 807, 1314, 1546, 1650, 1817
BtsI GCAGTG 2 cut(s) 60, 1386
BtsIMutI CAGTG 3 cut(s) 60, 1060, 1386
BveI ACCTGC 3 cut(s) 710, 1784, 1885
Cac8I GCNNGC 4 cut(s) 74, 570, 960, 1626
CfoI GCGC 1 cut(s) 1787
Cfr13I GGNCC 3 cut(s) 599, 766, 1247
Csp6I GTAC 6 cut(s) 167, 268, 292, 495, 1288, 1609
CspCI CAANNNNNGTGG 4 cut(s) 74, 109, 131, 166
CviQI GTAC 6 cut(s) 167, 268, 292, 495, 1288, 1609
DinI GGCGCC 1 cut(s) 1786
DpnI GATC 5 cut(s) 777, 945, 972, 1296, 1398
DpnII GATC 5 cut(s) 775, 943, 970, 1294, 1396
EaeI YGGCCR 1 cut(s) 679
Eam1104I CTCTTC 6 cut(s) 418, 640, 1194, 1495, 1855, 1904
EarI CTCTTC 6 cut(s) 418, 640, 1194, 1495, 1855, 1904
Eco31I GGTCTC 2 cut(s) 1342, 1550
Eco47I GGWCC 2 cut(s) 766, 1247
Eco57I CTGAAG 2 cut(s) 770, 1656
Eco72I CACGTG 1 cut(s) 1138
Eco81I CCTNAGG 1 cut(s) 1243
Eco88I CYCGRG 2 cut(s) 287, 1352
EcoO109I RGGNCCY 2 cut(s) 599, 766
EcoRII CCWGG 5 cut(s) 174, 1099, 1317, 1467, 1831
EcoT22I ATGCAT 2 cut(s) 960, 1286
EgeI GGCGCC 1 cut(s) 1786
EheI GGCGCC 1 cut(s) 1786
FaqI GGGAC 1 cut(s) 498
FblI GTMKAC 2 cut(s) 1221, 1253
Fnu4HI GCNGC 8 cut(s) 130, 242, 535, 982, 1524, 1527, 1757, 1827
FokI GGATG 7 cut(s) 259, 334, 794, 1301, 1553, 1657, 1804
Fsp4HI GCNGC 8 cut(s) 130, 242, 535, 982, 1524, 1527, 1757, 1827
FspBI CTAG 8 cut(s) 521, 642, 698, 888, 1298, 1677, 1734, 1809
GlaI GCGC 1 cut(s) 1786
GluI GCNGC 8 cut(s) 130, 242, 535, 982, 1524, 1527, 1757, 1827
GsaI CCCAGC 1 cut(s) 1539
GsuI CTGGAG 1 cut(s) 644
HaeII RGCGCY 1 cut(s) 1788
HaeIII GGCC 2 cut(s) 601, 681
HapII CCGG 1 cut(s) 1821
HhaI GCGC 1 cut(s) 1787
Hin1I GRCGYC 1 cut(s) 1785
Hin6I GCGC 1 cut(s) 1785
HinP1I GCGC 1 cut(s) 1785
HincII GTYRAC 1 cut(s) 1163
HindII GTYRAC 1 cut(s) 1163
HindIII AAGCTT 1 cut(s) 551
HpaII CCGG 1 cut(s) 1821
HphI GGTGA 1 cut(s) 1117
Hpy166II GTNNAC 8 cut(s) 342, 497, 916, 1163, 1177, 1222, 1254, 1478
Hpy188I TCNGA 7 cut(s) 192, 372, 481, 607, 820, 970, 1153
Hpy188III TCNNGA 8 cut(s) 299, 385, 521, 642, 661, 1067, 1235, 1659
Hpy8I GTNNAC 8 cut(s) 342, 497, 916, 1163, 1177, 1222, 1254, 1478
HpyAV CCTTC 5 cut(s) 536, 569, 612, 737, 1466
HpyCH4III ACNGT 6 cut(s) 382, 517, 858, 1084, 1510, 1721
HpyCH4IV ACGT 4 cut(s) 78, 525, 1137, 1179
HpySE526I ACGT 4 cut(s) 78, 525, 1137, 1179
Hsp92I GRCGYC 1 cut(s) 1785
HspAI GCGC 1 cut(s) 1785
KasI GGCGCC 1 cut(s) 1784
Kzo9I GATC 5 cut(s) 775, 943, 970, 1294, 1396
LguI GCTCTTC 3 cut(s) 1495, 1855, 1904
Lsp1109I GCAGC 6 cut(s) 116, 253, 546, 1535, 1538, 1768
LweI GCATC 3 cut(s) 1103, 1526, 1604
MaeI CTAG 8 cut(s) 521, 642, 698, 888, 1298, 1677, 1734, 1809
MaeII ACGT 4 cut(s) 78, 525, 1137, 1179
MaeIII GTNAC 5 cut(s) 218, 317, 511, 574, 1682
MalI GATC 5 cut(s) 777, 945, 972, 1296, 1398
MboI GATC 5 cut(s) 775, 943, 970, 1294, 1396
MfeI CAATTG 1 cut(s) 7
MflI RGATCY 1 cut(s) 1294
MhlI GDGCHC 5 cut(s) 74, 209, 344, 1480, 1486
MluCI AATT 8 cut(s) 7, 15, 433, 879, 1211, 1413, 1552, 1726
Mly113I GGCGCC 1 cut(s) 1785
MlyI GAGTC 5 cut(s) 382, 407, 657, 716, 1016
MmeI TCCRAC 3 cut(s) 988, 1162, 1740
Mph1103I ATGCAT 2 cut(s) 960, 1286
MseI TTAA 1 cut(s) 1751
MslI CAYNNNNRTG 2 cut(s) 1140, 1319
MspA1I CMGCKG 1 cut(s) 1405
MspI CCGG 1 cut(s) 1821
MspR9I CCNGG 5 cut(s) 176, 1101, 1319, 1469, 1833
MunI CAATTG 1 cut(s) 7
Mva1269I GAATGC 2 cut(s) 96, 453
MvaI CCWGG 5 cut(s) 176, 1101, 1319, 1469, 1833
NarI GGCGCC 1 cut(s) 1785
NdeII GATC 5 cut(s) 775, 943, 970, 1294, 1396
NlaIV GGNNCC 7 cut(s) 180, 600, 985, 1711, 1768, 1786, 1825
NmeAIII GCCGAG 1 cut(s) 91
NmuCI GTSAC 3 cut(s) 218, 317, 574
NsiI ATGCAT 2 cut(s) 960, 1286
NspI RCATGY 3 cut(s) 898, 962, 1288
PaeI GCATGC 1 cut(s) 962
PaqCI CACCTGC 2 cut(s) 710, 1885
PciSI GCTCTTC 3 cut(s) 1495, 1855, 1904
PctI GAATGC 2 cut(s) 96, 453
PfeI GAWTC 5 cut(s) 422, 476, 875, 1001, 1238
PflFI GACNNNGTC 1 cut(s) 489
PkrI GCNGC 8 cut(s) 131, 243, 536, 983, 1525, 1528, 1758, 1828
PleI GAGTC 5 cut(s) 382, 406, 656, 715, 1016
PluTI GGCGCC 1 cut(s) 1788
PmaCI CACGTG 1 cut(s) 1138
PmlI CACGTG 1 cut(s) 1138
PpsI GAGTC 5 cut(s) 382, 406, 656, 715, 1016
Ppu21I YACGTR 1 cut(s) 1138
PpuMI RGGWCCY 1 cut(s) 766
Psp5II RGGWCCY 1 cut(s) 766
Psp6I CCWGG 5 cut(s) 174, 1099, 1317, 1467, 1831
PspCI CACGTG 1 cut(s) 1138
PspFI CCCAGC 1 cut(s) 1535
PspGI CCWGG 5 cut(s) 174, 1099, 1317, 1467, 1831
PspN4I GGNNCC 7 cut(s) 180, 600, 985, 1711, 1768, 1786, 1825
PspPI GGNCC 3 cut(s) 599, 766, 1247
PspPPI RGGWCCY 1 cut(s) 766
PstI CTGCAG 4 cut(s) 421, 1372, 1795, 1858
PsuI RGATCY 1 cut(s) 1294
PsyI GACNNNGTC 1 cut(s) 489
RsaI GTAC 6 cut(s) 168, 269, 293, 496, 1289, 1610
RsaNI GTAC 6 cut(s) 167, 268, 292, 495, 1288, 1609
RseI CAYNNNNRTG 2 cut(s) 1140, 1319
SapI GCTCTTC 3 cut(s) 1495, 1855, 1904
SaqAI TTAA 1 cut(s) 1751
SatI GCNGC 8 cut(s) 130, 242, 535, 982, 1524, 1527, 1757, 1827
Sau3AI GATC 5 cut(s) 775, 943, 970, 1294, 1396
Sau96I GGNCC 3 cut(s) 599, 766, 1247
SbfI CCTGCAGG 1 cut(s) 1795
SchI GAGTC 5 cut(s) 382, 407, 657, 716, 1016
ScrFI CCNGG 5 cut(s) 176, 1101, 1319, 1469, 1833
SdaI CCTGCAGG 1 cut(s) 1795
SduI GDGCHC 5 cut(s) 74, 209, 344, 1480, 1486
SfaNI GCATC 3 cut(s) 1103, 1526, 1604
SfcI CTRYAG 5 cut(s) 417, 618, 1368, 1791, 1854
SfoI GGCGCC 1 cut(s) 1786
SinI GGWCC 2 cut(s) 766, 1247
SmiMI CAYNNNNRTG 2 cut(s) 1140, 1319
SmlI CTYRAG 2 cut(s) 563, 1530
SmoI CTYRAG 2 cut(s) 563, 1530
SphI GCATGC 1 cut(s) 962
Sse8387I CCTGCAGG 1 cut(s) 1795
Sse9I AATT 8 cut(s) 7, 15, 433, 879, 1211, 1413, 1552, 1726
SsiI CCGC 7 cut(s) 12, 464, 981, 1331, 1405, 1737, 1827
SspDI GGCGCC 1 cut(s) 1784
SspMI CTAG 8 cut(s) 521, 642, 698, 888, 1298, 1677, 1734, 1809
StyD4I CCNGG 5 cut(s) 174, 1099, 1317, 1467, 1831
TaaI ACNGT 6 cut(s) 382, 517, 858, 1084, 1510, 1721
TaiI ACGT 4 cut(s) 81, 528, 1140, 1182
TaqI TCGA 2 cut(s) 1399, 1660
TasI AATT 8 cut(s) 7, 15, 433, 879, 1211, 1413, 1552, 1726
TatI WGTACW 1 cut(s) 1287
TauI GCSGC 2 cut(s) 984, 1829
TfiI GAWTC 5 cut(s) 422, 476, 875, 1001, 1238
Tru1I TTAA 1 cut(s) 1751
Tru9I TTAA 1 cut(s) 1751
TscAI CASTG 3 cut(s) 67, 1060, 1386
TseFI GTSAC 3 cut(s) 218, 317, 574
TseI GCWGC 6 cut(s) 129, 241, 534, 1523, 1526, 1756
Tsp45I GTSAC 3 cut(s) 218, 317, 574
TspDTI ATGAA 6 cut(s) 17, 339, 918, 985, 1323, 1806
TspGWI ACGGA 1 cut(s) 1239
TspRI CASTG 3 cut(s) 67, 1060, 1386
Tth111I GACNNNGTC 1 cut(s) 489
VneI GTGCAC 2 cut(s) 340, 1476
VpaK11BI GGWCC 2 cut(s) 766, 1247
XapI RAATTY 3 cut(s) 15, 433, 1726
XbaI TCTAGA 2 cut(s) 520, 641
XceI RCATGY 3 cut(s) 898, 962, 1288
XmiI GTMKAC 2 cut(s) 1221, 1253
XspI CTAG 8 cut(s) 521, 642, 698, 888, 1298, 1677, 1734, 1809
Zsp2I ATGCAT 2 cut(s) 960, 1286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.