Rroxscaffold_1G00058860

VWA / Hh protein intein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000360
Physical Location & Seq
Reverse (-)
80818424 .. 80827523
9100 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00058860.1

Sequence Viewer

Length: 1692 bp
ATGTCGCGACAATATAGTTTCCGGTCTCGGGGTCCATCTTCTCAAAACATTTGCTCCATCTGCTGGAGTAACTTGACAACAGGGCAAGGCCAAGCCATCTTCACTGCTGAGTGCTCACACTCTTTCCACTACCCTTGCATTGCCAACAATGTCCAGCATGGAAATCTTTGCTGCCCTGCCTGCAGAGCAAAATGGAATAAAAATGATTTACCTTTCCAAGTTCCCCCTCTTCAACAAAATAAAAAGAGTAAACATGACTTCGCCGATGATGAACCTCTCTCGTCCGCCACACTCCAAACTTCTGGCCCCCAGAATGTAACAGTCAAGACTCATACAGAGACCTCTGCTATCTCTGCTGCCGAATCACGCCCCCATTATCCTGTTCTAGTTAGCATTTGTGCACCACCCCTTCAAGATCCCGATGGTGAAGGCCGTACACCCATTGACCTTGTTACAATTCTGGACGTAAGCGGTAGCATGGTTGGCCAAAAGCTTGAACTTGTCAAGCAAGCTGTCAAATTCGTCATTGAAAACATGGGGACTTCAGACAGACTGTCTATAGTATCATTCTCAGCAACTTCTAAACGACTCCTTCCTCTCCGTAGAATGACTGTTGAGGGCCGTGAAAGTGCAATTCAAGCTGTCGAATCTCTTAGAGCAGATGGTGGAACTGACATTGCAGAAGGACTCAAGATAGGAACTCATATCCTTGAGGAGCGAAGGGAAAGGAACCCTGTTGCTAGTATCATCCTCTTATCGGATGGCCTAGATAACTACTGTGACAACCCAAGCCAAATGTTGAAAAAATTGCCTGCTTCAATTCGTTCTAGTGACATGCAACATGAAATCCCAGTCCACACATTTGGTGTTGTGGCTCAGGAACTTCGCCTCACGGTGAGGTCAGCATCACCTGGGGTGAAGATCGTAGAAATACCATCAGGGAGACATGTCAGTCAGATTTCTGATGAGCAAGGTGTTGTTGATGTTGGAAATATGTATGCAGAAGAGGAGAAACAGTTTCTGGTGTATCTCTCAGTTCCACAATCCGCAGCTACAGATACCAAGACGTCTTTGGTAGACGTTTTGTGCGTGTACAAAGATCTTGGTTCAGATGAGTTTATCCCGGTCCAAGGTGAGAAAGTAGAGATAAGGAGACCTGATTTTTGTTCTCCTGCAGAAATGGCAGTGTCTTTGGAGGTTGATCGGCAGCGGAACAGACTTTTGGTGGCTGATGCTATTGGAGAGGCACAAAGGTTGGCTGAAATGGGCAATCTGGAGGCTGCACGGGCGGTTTTGACTCAGCGAAGAGAAACTCTCTTGACATCAACAGCAGCCAAAGCTGGGGATGATCTGAGTCATATGTTTGAAACTGAGCTTAAACAAATCATGGATAAAATGGCAAGTATGAATTTGTATGAAGAGGTGGGGCGTGCTTACGCTCTCTCAGGGATGAGTTCCCATGCACGGCAGAGGGCCTCGGCTAGGGGTAACGCAGCAGGCTCGTGCCTGGCCGAGAAGGCAGGTCGCAAACAACTTGCTACTAAAGCAGCGGGTCGTGCTACTTTTGAAACACCCGCTATGGTTAGAATGGATGGAGCTAATGAAATCAATGCTGATGTGAGCAAACACAAGAAGGCCATGCTAGTATACTGCGGGGGTCGGTATACTGCGGGGATTAGTACACTACGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VWA Vwaint zf-RING_11 zf-RING_2

Protein Analysis

563

Amino Acids

61.35

Weight (kDa)

6.69

Isoelectric Point (pI)

49.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 17 - 62 4.5e-06 Ring finger domain
zf-RING_11 PF17123 18 - 47 3e-09 RING-like zinc finger
zf-C3HC4 PF00097 18 - 61 1e-05 Zinc finger, C3HC4 type (RING finger)
VWA PF00092 149 - 323 1.1e-17 von Willebrand factor type A domain
VWA_3 PF13768 149 - 290 6.3e-12 von Willebrand factor type A domain
VWA_2 PF13519 150 - 255 2.2e-17 von Willebrand factor type A domain
Vwaint PF14624 455 - 530 6.5e-11 VWA / Hh protein intein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000360)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11691 FvH4_3g11693 FvH4_3g11740 FvH4_3g11750 FvH4_3g11770 FvH4_3g11771 FvH4_3g11772 FvH4_3g11780
malus_domestica MD10G1239000.v1.1
prunus_persica Prupe.4G105400_v2.0.a1
pyrus_communis pycom10g20000
rosa_chinensis RchiOBHm_Chr5g0018981 RchiOBHm_Chr5g0018991 RchiOBHm_Chr5g0019101 RchiOBHm_Chr5g0019111 RchiOBHm_Chr5g0019121 RchiOBHm_Chr5g0019151 RchiOBHm_Chr5g0019161 RchiOBHm_Chr5g0019171 RchiOBHm_Chr5g0019181 RchiOBHm_Chr5g0019201
rosa_laevigata RLG00000032411 RLG00000032418 RLG00000032419 RLG00000032420 RLG00000032421 RLG00000032423 RLG00000032424 RLG00000032426
rosa_multiflora Rmu_co8475613.1_g000001 Rmu_co8503587.1_g000001 Rmu_sc0000563.1_g000023 Rmu_sc0000563.1_g000027 Rmu_sc0002142.1_g000001 Rmu_sc0002142.1_g000003 Rmu_sc0002142.1_g000004 Rmu_sc0002142.1_g000011 Rmu_sc0002142.1_g000022 Rmu_sc0002561.1_g000002 Rmu_sc0042836.1_g000001
rosa_roxburghii Rroxscaffold_1G00058790 Rroxscaffold_1G00058810 Rroxscaffold_1G00058820 Rroxscaffold_1G00058840 Rroxscaffold_1G00058850 Rroxscaffold_1G00058860 Rroxscaffold_1G00058880 Rroxscaffold_1G00058910 Rroxscaffold_1G00074090 Rroxscaffold_1G00074110 Rroxscaffold_1G00074120 Rroxscaffold_1G00074130 Rroxscaffold_1G00074150
rosa_rugosa Rorug05G0046800 Rorug05G0047200 Rorug05G0047300 Rorug05G0047400 Rorug05G0047600 Rorug05G0047600 Rorug05G0047800 Rorug05G0047900 Rorug05G0048000 Rorug05G0048100 Rorug05G0048100 Rorug05G0048200 Rorug05G0048300
rosa_samantha Rh4DG102800 Rh5AG138700 Rh5AG138900 Rh5AG139000 Rh5AG139100 Rh5AG139200 Rh5AG139400 Rh5AG139500 Rh5AG139600 Rh5BG137400 Rh5BG137600 Rh5BG137800 Rh5BG138100 Rh5BG138200 Rh5BG138300 Rh5BG138500 Rh5CG148800 Rh5CG148900 Rh5CG149200 Rh5CG149300 Rh5CG149400 Rh5CG149600 Rh5CG149700 Rh5CG150000 Rh5DG138200 Rh5DG138300 Rh5DG138500 Rh5DG138600 Rh5DG138700 Rh5DG138900 Rh5DG139000 Rh5DG139200
rosa_wichuraiana Rw5G012290 Rw5G012330 Rw5G012340 Rw5G012350 Rw5G012370 Rw5G012380 Rw5G012390 Rw5G012410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 951
AatII GACGTC 1 cut(s) 1070
Acc36I ACCTGC 1 cut(s) 1511
AccB7I CCANNNNNTGG 1 cut(s) 63
AccI GTMKAC 3 cut(s) 1077, 1647, 1664
AccII CGCG 1 cut(s) 7
AciI CCGC 9 cut(s) 285, 471, 1047, 1210, 1289, 1550, 1575, 1653, 1670
AclWI GGATC 1 cut(s) 410
AcoI YGGCCR 2 cut(s) 484, 1509
AcsI RAATTY 2 cut(s) 518, 1408
AcuI CTGAAG 1 cut(s) 528
AcyI GRCGYC 1 cut(s) 1067
AfaI GTAC 3 cut(s) 436, 1094, 1681
AfiI CCNNNNNNNGG 7 cut(s) 28, 63, 757, 1130, 1341, 1464, 1482
AflIII ACRYGT 1 cut(s) 946
AgsI TTSAA 9 cut(s) 233, 413, 497, 530, 638, 802, 819, 1367, 1568
AhdI GACNNNNNGTC 1 cut(s) 553
AjnI CCWGG 2 cut(s) 910, 1506
AjuI GAANNNNNNNTTGG 2 cut(s) 1205, 1237
AluBI AGCT 7 cut(s) 493, 512, 641, 1052, 1340, 1375, 1598
AluI AGCT 7 cut(s) 493, 512, 641, 1052, 1340, 1375, 1598
Alw21I GWGCWC 2 cut(s) 116, 403
Alw26I GTCTC 4 cut(s) 30, 332, 937, 1147
Alw44I GTGCAC 1 cut(s) 399
AlwI GGATC 1 cut(s) 410
AlwNI CAGNNNCTG 1 cut(s) 1021
Ama87I CYCGRG 1 cut(s) 27
AoxI GGCC 9 cut(s) 88, 304, 430, 484, 619, 763, 1473, 1509, 1635
ApaLI GTGCAC 1 cut(s) 399
ApeKI GCWGC 8 cut(s) 171, 356, 1049, 1207, 1280, 1331, 1493, 1547
ApoI RAATTY 2 cut(s) 518, 1408
ArsI GACNNNNNNTTYG 2 cut(s) 1146, 1178
AspS9I GGNCC 5 cut(s) 32, 305, 619, 1126, 1473
AsuC2I CCSGG 1 cut(s) 1124
AsuHPI GGTGA 5 cut(s) 437, 900, 907, 928, 1145
AvaI CYCGRG 1 cut(s) 27
AvaII GGWCC 2 cut(s) 32, 1126
BaeGI GKGCMC 1 cut(s) 403
BalI TGGCCA 1 cut(s) 486
BauI CACGAG 1 cut(s) 1501
Bbv12I GWGCWC 2 cut(s) 116, 403
BbvI GCAGC 8 cut(s) 158, 343, 1061, 1219, 1267, 1343, 1505, 1559
BccI CCATC 8 cut(s) 43, 65, 104, 416, 656, 755, 943, 1586
BceAI ACGGC 3 cut(s) 417, 606, 1481
BcgI CGANNNNNNTGC 2 cut(s) 1482, 1516
BciT130I CCWGG 2 cut(s) 912, 1508
BcnI CCSGG 1 cut(s) 1124
BcoDI GTCTC 4 cut(s) 30, 332, 937, 1147
BfaI CTAG 6 cut(s) 386, 741, 767, 828, 1482, 1643
BfmI CTRYAG 4 cut(s) 181, 558, 1053, 1173
BfuAI ACCTGC 1 cut(s) 1511
BglI GCCNNNNNGGC 1 cut(s) 1517
BglII AGATCT 1 cut(s) 1099
BisI GCNGC 8 cut(s) 172, 357, 1050, 1208, 1281, 1332, 1494, 1548
BlsI GCNGC 8 cut(s) 173, 358, 1051, 1209, 1282, 1333, 1495, 1549
Bme1390I CCNGG 3 cut(s) 912, 1124, 1508
Bme18I GGWCC 2 cut(s) 32, 1126
BmeRI GACNNNNNGTC 1 cut(s) 553
BmeT110I CYCGRG 1 cut(s) 27
BmgT120I GGNCC 5 cut(s) 32, 305, 619, 1126, 1473
BmiI GGNNCC 3 cut(s) 33, 307, 731
BmrFI CCNGG 3 cut(s) 912, 1124, 1508
BmrI ACTGGG 1 cut(s) 845
BmsI GCATC 2 cut(s) 914, 1222
BmuI ACTGGG 1 cut(s) 845
BplI GAGNNNNNCTC 2 cut(s) 1299, 1331
BpmI CTGGAG 2 cut(s) 85, 1295
Bpu10I CCTNAGC 1 cut(s) 876
BpuEI CTTGAG 2 cut(s) 674, 731
BpuMI CCSGG 1 cut(s) 1124
BsaHI GRCGYC 1 cut(s) 1067
BsaI GGTCTC 3 cut(s) 30, 332, 1147
BsaJI CCNNGG 3 cut(s) 911, 1129, 1476
BsaWI WCCGGW 1 cut(s) 21
BsaXI ACNNNNNCTCC 4 cut(s) 38, 68, 1001, 1031
Bsc4I CCNNNNNNNGG 7 cut(s) 28, 63, 757, 1130, 1341, 1464, 1482
Bse1I ACTGG 1 cut(s) 851
Bse3DI GCAATG 2 cut(s) 138, 675
BseBI CCWGG 2 cut(s) 912, 1508
BseDI CCNNGG 3 cut(s) 911, 1129, 1476
BseGI GGATG 5 cut(s) 747, 766, 1351, 1455, 1597
BseLI CCNNNNNNNGG 7 cut(s) 28, 63, 757, 1130, 1341, 1464, 1482
BseMI GCAATG 2 cut(s) 138, 675
BseMII CTCAG 8 cut(s) 99, 585, 890, 1047, 1313, 1343, 1362, 1458
BseNI ACTGG 1 cut(s) 851
BseRI GAGGAG 2 cut(s) 728, 1022
BseSI GKGCMC 1 cut(s) 403
BseXI GCAGC 8 cut(s) 158, 343, 1061, 1219, 1267, 1343, 1505, 1559
BseYI CCCAGC 1 cut(s) 1340
BsgI GTGCAG 1 cut(s) 1266
Bsh1236I CGCG 1 cut(s) 7
BshFI GGCC 9 cut(s) 90, 306, 432, 486, 621, 765, 1475, 1511, 1637
BsiHKAI GWGCWC 2 cut(s) 116, 403
BsiHKCI CYCGRG 1 cut(s) 27
BsiSI CCGG 2 cut(s) 22, 1124
BslFI GGGAC 1 cut(s) 553
BslI CCNNNNNNNGG 7 cut(s) 28, 63, 757, 1130, 1341, 1464, 1482
BsmAI GTCTC 4 cut(s) 30, 332, 937, 1147
BsmFI GGGAC 1 cut(s) 553
BsnI GGCC 9 cut(s) 90, 306, 432, 486, 621, 765, 1475, 1511, 1637
Bso31I GGTCTC 3 cut(s) 30, 332, 1147
BsoBI CYCGRG 1 cut(s) 27
Bsp1286I GDGCHC 2 cut(s) 116, 403
Bsp1407I TGTACA 1 cut(s) 1092
Bsp143I GATC 5 cut(s) 415, 921, 1099, 1201, 1348
Bsp68I TCGCGA 1 cut(s) 7
BspACI CCGC 9 cut(s) 285, 471, 1047, 1210, 1289, 1550, 1575, 1653, 1670
BspANI GGCC 9 cut(s) 90, 306, 432, 486, 621, 765, 1475, 1511, 1637
BspCNI CTCAG 8 cut(s) 100, 584, 889, 1046, 1312, 1344, 1363, 1457
BspFNI CGCG 1 cut(s) 7
BspLI GGNNCC 3 cut(s) 33, 307, 731
BspMAI CTGCAG 2 cut(s) 185, 1177
BspMI ACCTGC 1 cut(s) 1511
BspPI GGATC 1 cut(s) 410
BspTNI GGTCTC 3 cut(s) 30, 332, 1147
BsrDI GCAATG 2 cut(s) 138, 675
BsrGI TGTACA 1 cut(s) 1092
BsrI ACTGG 1 cut(s) 851
BssECI CCNNGG 3 cut(s) 911, 1129, 1476
BssMI GATC 5 cut(s) 415, 921, 1099, 1201, 1348
BssNAI GTATAC 2 cut(s) 1648, 1665
BssNI GRCGYC 1 cut(s) 1067
BssSI CACGAG 1 cut(s) 1501
BssT1I CCWWGG 1 cut(s) 1129
Bst1107I GTATAC 2 cut(s) 1648, 1665
Bst2BI CACGAG 1 cut(s) 1501
Bst2UI CCWGG 2 cut(s) 912, 1508
Bst4CI ACNGT 6 cut(s) 322, 555, 613, 779, 895, 1017
Bst6I CTCTTC 4 cut(s) 234, 999, 1300, 1413
BstACI GRCGYC 1 cut(s) 1067
BstAUI TGTACA 1 cut(s) 1092
BstC8I GCNNGC 5 cut(s) 181, 510, 813, 1431, 1498
BstDEI CTNAG 9 cut(s) 108, 571, 653, 876, 1033, 1299, 1352, 1371, 1444
BstENI CCTNNNNNAGG 1 cut(s) 1480
BstF5I GGATG 5 cut(s) 747, 766, 1351, 1455, 1597
BstFNI CGCG 1 cut(s) 7
BstKTI GATC 5 cut(s) 418, 924, 1102, 1204, 1351
BstMAI GTCTC 4 cut(s) 30, 332, 937, 1147
BstMBI GATC 5 cut(s) 415, 921, 1099, 1201, 1348
BstNI CCWGG 2 cut(s) 912, 1508
BstNSI RCATGY 2 cut(s) 838, 950
BstSCI CCNGG 3 cut(s) 910, 1122, 1506
BstSFI CTRYAG 4 cut(s) 181, 558, 1053, 1173
BstSLI GKGCMC 1 cut(s) 403
BstUI CGCG 1 cut(s) 7
BstV1I GCAGC 8 cut(s) 158, 343, 1061, 1219, 1267, 1343, 1505, 1559
BstX2I RGATCY 2 cut(s) 415, 1099
BstXI CCANNNNNNTGG 2 cut(s) 302, 863
BstYI RGATCY 2 cut(s) 415, 1099
BstZ17I GTATAC 2 cut(s) 1648, 1665
BsuRI GGCC 9 cut(s) 90, 306, 432, 486, 621, 765, 1475, 1511, 1637
BtsCI GGATG 5 cut(s) 747, 766, 1351, 1455, 1597
BtsI GCAGTG 2 cut(s) 102, 1191
BtsIMutI CAGTG 2 cut(s) 102, 1191
BtuMI TCGCGA 1 cut(s) 7
BveI ACCTGC 1 cut(s) 1511
Cac8I GCNNGC 5 cut(s) 181, 510, 813, 1431, 1498
CaiI CAGNNNCTG 1 cut(s) 1021
Cfr13I GGNCC 5 cut(s) 32, 305, 619, 1126, 1473
Csp6I GTAC 3 cut(s) 435, 1093, 1680
CspCI CAANNNNNGTGG 2 cut(s) 116, 151
CviQI GTAC 3 cut(s) 435, 1093, 1680
DdeI CTNAG 9 cut(s) 108, 571, 653, 876, 1033, 1299, 1352, 1371, 1444
DpnI GATC 5 cut(s) 417, 923, 1101, 1203, 1350
DpnII GATC 5 cut(s) 415, 921, 1099, 1201, 1348
DrdI GACNNNNNNGTC 1 cut(s) 951
DriI GACNNNNNGTC 1 cut(s) 553
DseDI GACNNNNNNGTC 1 cut(s) 951
EaeI YGGCCR 2 cut(s) 484, 1509
Eam1104I CTCTTC 4 cut(s) 234, 999, 1300, 1413
Eam1105I GACNNNNNGTC 1 cut(s) 553
EarI CTCTTC 4 cut(s) 234, 999, 1300, 1413
EciI GGCGGA 1 cut(s) 274
Eco130I CCWWGG 1 cut(s) 1129
Eco31I GGTCTC 3 cut(s) 30, 332, 1147
Eco47I GGWCC 2 cut(s) 32, 1126
Eco57I CTGAAG 1 cut(s) 528
Eco88I CYCGRG 1 cut(s) 27
EcoNI CCTNNNNNAGG 1 cut(s) 1480
EcoO109I RGGNCCY 1 cut(s) 1473
EcoRII CCWGG 2 cut(s) 910, 1506
EcoT14I CCWWGG 1 cut(s) 1129
ErhI CCWWGG 1 cut(s) 1129
FaqI GGGAC 1 cut(s) 553
FauI CCCGC 4 cut(s) 1543, 1582, 1646, 1663
FauNDI CATATG 1 cut(s) 1359
FblI GTMKAC 3 cut(s) 1077, 1647, 1664
Fnu4HI GCNGC 8 cut(s) 172, 357, 1050, 1208, 1281, 1332, 1494, 1548
FokI GGATG 5 cut(s) 734, 773, 1358, 1462, 1604
Fsp4HI GCNGC 8 cut(s) 172, 357, 1050, 1208, 1281, 1332, 1494, 1548
FspBI CTAG 6 cut(s) 386, 741, 767, 828, 1482, 1643
GluI GCNGC 8 cut(s) 172, 357, 1050, 1208, 1281, 1332, 1494, 1548
GsaI CCCAGC 1 cut(s) 1344
GsuI CTGGAG 2 cut(s) 85, 1295
HaeIII GGCC 9 cut(s) 90, 306, 432, 486, 621, 765, 1475, 1511, 1637
HapII CCGG 2 cut(s) 22, 1124
Hin1I GRCGYC 1 cut(s) 1067
HindIII AAGCTT 1 cut(s) 491
HinfI GANTC 7 cut(s) 328, 362, 588, 647, 687, 1297, 1354
HpaII CCGG 2 cut(s) 22, 1124
HphI GGTGA 5 cut(s) 437, 900, 907, 928, 1145
Hpy166II GTNNAC 9 cut(s) 251, 401, 437, 856, 1078, 1093, 1648, 1665, 1682
Hpy188I TCNGA 6 cut(s) 547, 760, 957, 964, 1111, 1353
Hpy188III TCNNGA 9 cut(s) 6, 325, 413, 419, 461, 691, 878, 1274, 1318
Hpy8I GTNNAC 9 cut(s) 251, 401, 437, 856, 1078, 1093, 1648, 1665, 1682
HpyAV CCTTC 7 cut(s) 419, 422, 602, 677, 714, 1510, 1627
HpyCH4III ACNGT 6 cut(s) 322, 555, 613, 779, 895, 1017
HpyCH4IV ACGT 3 cut(s) 465, 1067, 1080
HpyF3I CTNAG 9 cut(s) 108, 571, 653, 876, 1033, 1299, 1352, 1371, 1444
HpySE526I ACGT 3 cut(s) 465, 1067, 1080
Hsp92I GRCGYC 1 cut(s) 1067
Kzo9I GATC 5 cut(s) 415, 921, 1099, 1201, 1348
LmnI GCTCC 3 cut(s) 59, 715, 1595
Lsp1109I GCAGC 8 cut(s) 158, 343, 1061, 1219, 1267, 1343, 1505, 1559
LweI GCATC 2 cut(s) 914, 1222
MaeI CTAG 6 cut(s) 386, 741, 767, 828, 1482, 1643
MaeII ACGT 3 cut(s) 465, 1067, 1080
MaeIII GTNAC 6 cut(s) 68, 316, 451, 779, 830, 1487
MalI GATC 5 cut(s) 417, 923, 1101, 1203, 1350
MboI GATC 5 cut(s) 415, 921, 1099, 1201, 1348
MboII GAAGA 7 cut(s) 30, 91, 221, 931, 1016, 1317, 1430
MflI RGATCY 2 cut(s) 415, 1099
MhlI GDGCHC 2 cut(s) 116, 403
MlsI TGGCCA 1 cut(s) 486
MluCI AATT 6 cut(s) 456, 518, 633, 806, 819, 1408
MluNI TGGCCA 1 cut(s) 486
MlyI GAGTC 5 cut(s) 322, 582, 681, 1291, 1363
MmeI TCCRAC 1 cut(s) 967
Mox20I TGGCCA 1 cut(s) 486
MscI TGGCCA 1 cut(s) 486
MseI TTAA 1 cut(s) 1377
Msp20I TGGCCA 1 cut(s) 486
MspA1I CMGCKG 2 cut(s) 1210, 1550
MspI CCGG 2 cut(s) 22, 1124
MspR9I CCNGG 3 cut(s) 912, 1124, 1508
MvaI CCWGG 2 cut(s) 912, 1508
MvnI CGCG 1 cut(s) 7
NciI CCSGG 1 cut(s) 1124
NdeI CATATG 1 cut(s) 1359
NdeII GATC 5 cut(s) 415, 921, 1099, 1201, 1348
NlaIV GGNNCC 3 cut(s) 33, 307, 731
NmeAIII GCCGAG 2 cut(s) 1457, 1537
NmuCI GTSAC 2 cut(s) 779, 830
NruI TCGCGA 1 cut(s) 7
NspI RCATGY 2 cut(s) 838, 950
PciI ACATGT 1 cut(s) 946
PcsI WCGNNNNNNNCGW 1 cut(s) 1086
PfeI GAWTC 2 cut(s) 362, 647
PflMI CCANNNNNTGG 1 cut(s) 63
PkrI GCNGC 8 cut(s) 173, 358, 1051, 1209, 1282, 1333, 1495, 1549
PleI GAGTC 5 cut(s) 322, 582, 681, 1291, 1362
PpsI GAGTC 5 cut(s) 322, 582, 681, 1291, 1362
PscI ACATGT 1 cut(s) 946
Psp6I CCWGG 2 cut(s) 910, 1506
PspFI CCCAGC 1 cut(s) 1340
PspGI CCWGG 2 cut(s) 910, 1506
PspN4I GGNNCC 3 cut(s) 33, 307, 731
PspPI GGNCC 5 cut(s) 32, 305, 619, 1126, 1473
PstI CTGCAG 2 cut(s) 185, 1177
PstNI CAGNNNCTG 1 cut(s) 1021
PsuI RGATCY 2 cut(s) 415, 1099
RruI TCGCGA 1 cut(s) 7
RsaI GTAC 3 cut(s) 436, 1094, 1681
RsaNI GTAC 3 cut(s) 435, 1093, 1680
SaqAI TTAA 1 cut(s) 1377
SatI GCNGC 8 cut(s) 172, 357, 1050, 1208, 1281, 1332, 1494, 1548
Sau3AI GATC 5 cut(s) 415, 921, 1099, 1201, 1348
Sau96I GGNCC 5 cut(s) 32, 305, 619, 1126, 1473
SchI GAGTC 5 cut(s) 322, 582, 681, 1291, 1363
ScrFI CCNGG 3 cut(s) 912, 1124, 1508
SduI GDGCHC 2 cut(s) 116, 403
SfaNI GCATC 2 cut(s) 914, 1222
SfcI CTRYAG 4 cut(s) 181, 558, 1053, 1173
SinI GGWCC 2 cut(s) 32, 1126
SmlI CTYRAG 2 cut(s) 689, 710
SmoI CTYRAG 2 cut(s) 689, 710
Sse9I AATT 6 cut(s) 456, 518, 633, 806, 819, 1408
SsiI CCGC 9 cut(s) 285, 471, 1047, 1210, 1289, 1550, 1575, 1653, 1670
SspMI CTAG 6 cut(s) 386, 741, 767, 828, 1482, 1643
StyD4I CCNGG 3 cut(s) 910, 1122, 1506
StyI CCWWGG 1 cut(s) 1129
TaaI ACNGT 6 cut(s) 322, 555, 613, 779, 895, 1017
TaiI ACGT 3 cut(s) 468, 1070, 1083
TaqI TCGA 1 cut(s) 645
TasI AATT 6 cut(s) 456, 518, 633, 806, 819, 1408
TatI WGTACW 2 cut(s) 1092, 1679
TfiI GAWTC 2 cut(s) 362, 647
Tru1I TTAA 1 cut(s) 1377
Tru9I TTAA 1 cut(s) 1377
TscAI CASTG 2 cut(s) 109, 1191
TseFI GTSAC 2 cut(s) 779, 830
TseI GCWGC 8 cut(s) 171, 356, 1049, 1207, 1280, 1331, 1493, 1547
Tsp45I GTSAC 2 cut(s) 779, 830
TspDTI ATGAA 5 cut(s) 285, 858, 1421, 1431, 1617
TspGWI ACGGA 1 cut(s) 590
TspRI CASTG 2 cut(s) 109, 1191
Van91I CCANNNNNTGG 1 cut(s) 63
VneI GTGCAC 1 cut(s) 399
VpaK11BI GGWCC 2 cut(s) 32, 1126
XagI CCTNNNNNAGG 1 cut(s) 1480
XapI RAATTY 2 cut(s) 518, 1408
XceI RCATGY 2 cut(s) 838, 950
XcmI CCANNNNNNNNNTGG 1 cut(s) 1069
XmiI GTMKAC 3 cut(s) 1077, 1647, 1664
XspI CTAG 6 cut(s) 386, 741, 767, 828, 1482, 1643
ZraI GACGTC 1 cut(s) 1068
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.