Rh5AG139200

VWA / Hh protein intein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000360
Physical Location & Seq
Forward (+)
14461885 .. 14463072
1188 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG139200.1

Sequence Viewer

Length: 1188 bp
ATGTCCGTTGATGGTCGTGAAAGTGCCATCCTAGCCATTGAGTCTCTTAGAGCAGGTGGTGCAACTAACATTGCAGAAGGACTGAAGAAAGGAACTAAGGTCCTTGAAGATCGAAGGGAAAGAAACCCAGTTTCCAGCATCATCCTCTTATCTGATGGCAAAGATACATACAATACAACTGCAAACCGACTGTTGAACCAGTTGCCTGATTCGATTCGTTCTAGCAACACGCAACAGGAAACCCCAGTCCACACATTTGGATTTGGCTACGATCATGATCCCAGCATTATGCATGCTATATCTGATGTGTCAGGTGGCACCTTTTCGTTCATTGAATCAGTTGAAATTATACAAGACTCCTTTGCTCTGTGTATTGGGGGTCTTCTCAGTGTTGTGGCTCAGGAAGTTCGCCTAACAGTAAGATCAGCATCACCTGGGGTGAAGATTTTAGCAATACCATCAGGGAGACATGTGAATGAGATTTCTGATGGTCAGCAAGGTGTTGTTCATGTTGGAAATATGTATGCTGAAGAGGAAAAACAATTTCTGGTCTACCTAACAGTTCCAGAATCGTCCGCTCCACATACTAAGACGTCATTGTTAGAGGTGTTATGCATGTACAAAGATCTAGCTTCAAATGAGTTGATGGAGGTGCAAGGTGAGAAAATAGAGATACTGAGACCTGAGGTTTGTTCCCCTGCTGAAAAGACTGTTTCTTTGGAGGTTGATCGGCAGCGGAACAGAATATTGGTGGCTGAAGCTATAGCTGAGGCACAAAGGTTGGCTGAGAATGGAAATCTGGAGGGTGCACAGGCTCTTTTGGCTCAACGGAAAGAAATTCTCTCAACATCAACAACAGCCCAAGCCGGGGATTTTCAGAGTAATTTGTTTGAAACTGAGCTTAAAGAAATGATGGATAGAATGGCAAGTATGAAGTTGTATAAACGAACCGGGCGTGCTTACGCTCTCTCAGGACTCAGTTCCCATAAGTTGCAAAGGGCCACAACTAGGAAGTGTCGTAGGGTTTCCCATAGTGGGGGCTTTGAAGGTCGCAAGGCTTCCGGTGCTTCATCAGGTGCTACTCCAGGTGAATCGCCTGCCGCCGTTGGTGCTTTTGAAACACCTGCTATGGTCAGAATGGTAGAAAAATCACAGAAGAGGAATCAACCTGCGCCAGACCAAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VWA Vwaint zf-RING_11 zf-RING_2

Protein Analysis

395

Amino Acids

42.83

Weight (kDa)

6.13

Isoelectric Point (pI)

52.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VWA_3 PF13768 6 - 110 1e-07 von Willebrand factor type A domain
VWA PF00092 8 - 113 9.3e-07 von Willebrand factor type A domain
WAV3_C PF25243 242 - 309 1e-06 E3 ubiquitin-protein ligase WAV3-like, C-terminal domain
Vwaint PF14624 299 - 386 1.3e-12 VWA / Hh protein intein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000360)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11691 FvH4_3g11693 FvH4_3g11740 FvH4_3g11750 FvH4_3g11770 FvH4_3g11771 FvH4_3g11772 FvH4_3g11780
malus_domestica MD10G1239000.v1.1
prunus_persica Prupe.4G105400_v2.0.a1
pyrus_communis pycom10g20000
rosa_chinensis RchiOBHm_Chr5g0018981 RchiOBHm_Chr5g0018991 RchiOBHm_Chr5g0019101 RchiOBHm_Chr5g0019111 RchiOBHm_Chr5g0019121 RchiOBHm_Chr5g0019151 RchiOBHm_Chr5g0019161 RchiOBHm_Chr5g0019171 RchiOBHm_Chr5g0019181 RchiOBHm_Chr5g0019201
rosa_laevigata RLG00000032411 RLG00000032418 RLG00000032419 RLG00000032420 RLG00000032421 RLG00000032423 RLG00000032424 RLG00000032426
rosa_multiflora Rmu_co8475613.1_g000001 Rmu_co8503587.1_g000001 Rmu_sc0000563.1_g000023 Rmu_sc0000563.1_g000027 Rmu_sc0002142.1_g000001 Rmu_sc0002142.1_g000003 Rmu_sc0002142.1_g000004 Rmu_sc0002142.1_g000011 Rmu_sc0002142.1_g000022 Rmu_sc0002561.1_g000002 Rmu_sc0042836.1_g000001
rosa_roxburghii Rroxscaffold_1G00058790 Rroxscaffold_1G00058810 Rroxscaffold_1G00058820 Rroxscaffold_1G00058840 Rroxscaffold_1G00058850 Rroxscaffold_1G00058860 Rroxscaffold_1G00058880 Rroxscaffold_1G00058910 Rroxscaffold_1G00074090 Rroxscaffold_1G00074110 Rroxscaffold_1G00074120 Rroxscaffold_1G00074130 Rroxscaffold_1G00074150
rosa_rugosa Rorug05G0046800 Rorug05G0047200 Rorug05G0047300 Rorug05G0047400 Rorug05G0047600 Rorug05G0047600 Rorug05G0047800 Rorug05G0047900 Rorug05G0048000 Rorug05G0048100 Rorug05G0048100 Rorug05G0048200 Rorug05G0048300
rosa_samantha Rh4DG102800 Rh5AG138700 Rh5AG138900 Rh5AG139000 Rh5AG139100 Rh5AG139200 Rh5AG139400 Rh5AG139500 Rh5AG139600 Rh5BG137400 Rh5BG137600 Rh5BG137800 Rh5BG138100 Rh5BG138200 Rh5BG138300 Rh5BG138500 Rh5CG148800 Rh5CG148900 Rh5CG149200 Rh5CG149300 Rh5CG149400 Rh5CG149600 Rh5CG149700 Rh5CG150000 Rh5DG138200 Rh5DG138300 Rh5DG138500 Rh5DG138600 Rh5DG138700 Rh5DG138900 Rh5DG139000 Rh5DG139200
rosa_wichuraiana Rw5G012290 Rw5G012330 Rw5G012340 Rw5G012350 Rw5G012370 Rw5G012380 Rw5G012390 Rw5G012410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 44, 1132
AatII GACGTC 1 cut(s) 596
Acc36I ACCTGC 3 cut(s) 44, 1132, 1177
AccB1I GGYRCC 1 cut(s) 317
AccBSI CCGCTC 1 cut(s) 578
AccI GTMKAC 1 cut(s) 552
AciI CCGC 3 cut(s) 576, 736, 1101
AclWI GGATC 1 cut(s) 272
AcsI RAATTY 1 cut(s) 837
AcuI CTGAAG 3 cut(s) 104, 549, 777
AcyI GRCGYC 1 cut(s) 593
AfaI GTAC 1 cut(s) 620
AfiI CCNNNNNNNGG 5 cut(s) 867, 868, 1008, 1035, 1036
AflIII ACRYGT 1 cut(s) 469
AgsI TTSAA 8 cut(s) 107, 196, 335, 344, 636, 893, 1046, 1118
AjnI CCWGG 2 cut(s) 433, 1084
AjuI GAANNNNNNNTTGG 2 cut(s) 731, 763
AluBI AGCT 4 cut(s) 632, 761, 767, 901
AluI AGCT 4 cut(s) 632, 761, 767, 901
Alw21I GWGCWC 1 cut(s) 811
Alw26I GTCTC 3 cut(s) 48, 460, 673
Alw44I GTGCAC 1 cut(s) 807
AlwI GGATC 1 cut(s) 272
AoxI GGCC 1 cut(s) 999
ApaLI GTGCAC 1 cut(s) 807
ApeKI GCWGC 1 cut(s) 733
ApoI RAATTY 1 cut(s) 837
ArsI GACNNNNNNTTYG 4 cut(s) 672, 700, 704, 732
AspLEI GCGC 1 cut(s) 1174
AspS9I GGNCC 2 cut(s) 100, 999
AsuC2I CCSGG 2 cut(s) 868, 952
AsuHPI GGTGA 4 cut(s) 423, 451, 671, 1100
AvaII GGWCC 1 cut(s) 100
AxyI CCTNAGG 1 cut(s) 684
BaeGI GKGCMC 1 cut(s) 811
BanI GGYRCC 1 cut(s) 317
BbsI GAAGAC 1 cut(s) 374
Bbv12I GWGCWC 1 cut(s) 811
BbvCI CCTCAGC 1 cut(s) 768
BbvI GCAGC 1 cut(s) 745
BccI CCATC 7 cut(s) 5, 35, 149, 466, 482, 640, 907
BceAI ACGGC 1 cut(s) 1088
BciT130I CCWGG 2 cut(s) 435, 1086
BcnI CCSGG 2 cut(s) 868, 952
BcoDI GTCTC 3 cut(s) 48, 460, 673
BfaI CTAG 4 cut(s) 32, 222, 629, 1008
BfmI CTRYAG 1 cut(s) 762
BfuAI ACCTGC 3 cut(s) 44, 1132, 1177
BglII AGATCT 1 cut(s) 625
BisI GCNGC 2 cut(s) 734, 1101
BlsI GCNGC 2 cut(s) 735, 1102
Bme1390I CCNGG 4 cut(s) 435, 868, 952, 1086
Bme18I GGWCC 1 cut(s) 100
BmgT120I GGNCC 2 cut(s) 100, 999
BmiI GGNNCC 1 cut(s) 319
BmrFI CCNGG 4 cut(s) 435, 868, 952, 1086
BmrI ACTGGG 2 cut(s) 122, 239
BmsI GCATC 2 cut(s) 147, 437
BmuI ACTGGG 2 cut(s) 122, 239
BpiI GAAGAC 1 cut(s) 374
BpmI CTGGAG 2 cut(s) 821, 1068
Bpu10I CCTNAGC 2 cut(s) 399, 768
BpuMI CCSGG 2 cut(s) 868, 952
BsaBI GATNNNNATC 2 cut(s) 276, 427
BsaHI GRCGYC 1 cut(s) 593
BsaI GGTCTC 1 cut(s) 673
BsaJI CCNNGG 2 cut(s) 434, 867
BsaWI WCCGGW 1 cut(s) 1061
Bsc4I CCNNNNNNNGG 5 cut(s) 867, 868, 1008, 1035, 1036
Bse1I ACTGG 3 cut(s) 128, 199, 245
Bse21I CCTNAGG 1 cut(s) 684
Bse3DI GCAATG 1 cut(s) 69
Bse8I GATNNNNATC 2 cut(s) 276, 427
BseBI CCWGG 2 cut(s) 435, 1086
BseDI CCNNGG 2 cut(s) 434, 867
BseGI GGATG 2 cut(s) 27, 141
BseJI GATNNNNATC 2 cut(s) 276, 427
BseLI CCNNNNNNNGG 5 cut(s) 867, 868, 1008, 1035, 1036
BseMI GCAATG 1 cut(s) 69
BseMII CTCAG 9 cut(s) 400, 413, 668, 675, 759, 777, 888, 984, 991
BseNI ACTGG 3 cut(s) 128, 199, 245
BseSI GKGCMC 1 cut(s) 811
BseXI GCAGC 1 cut(s) 745
BseYI CCCAGC 1 cut(s) 281
BshFI GGCC 1 cut(s) 1001
BshNI GGYRCC 1 cut(s) 317
BsiHKAI GWGCWC 1 cut(s) 811
BsiSI CCGG 3 cut(s) 867, 951, 1062
BslI CCNNNNNNNGG 5 cut(s) 867, 868, 1008, 1035, 1036
BsmAI GTCTC 3 cut(s) 48, 460, 673
BsnI GGCC 1 cut(s) 1001
Bso31I GGTCTC 1 cut(s) 673
Bsp1286I GDGCHC 1 cut(s) 811
Bsp1407I TGTACA 1 cut(s) 618
Bsp143I GATC 6 cut(s) 109, 271, 277, 422, 625, 727
BspACI CCGC 3 cut(s) 576, 736, 1101
BspANI GGCC 1 cut(s) 1001
BspCNI CTCAG 9 cut(s) 399, 412, 669, 676, 760, 778, 889, 983, 990
BspHI TCATGA 1 cut(s) 274
BspLI GGNNCC 1 cut(s) 319
BspMI ACCTGC 3 cut(s) 44, 1132, 1177
BspPI GGATC 1 cut(s) 272
BspT107I GGYRCC 1 cut(s) 317
BspTNI GGTCTC 1 cut(s) 673
BsrBI CCGCTC 1 cut(s) 578
BsrDI GCAATG 1 cut(s) 69
BsrGI TGTACA 1 cut(s) 618
BsrI ACTGG 3 cut(s) 128, 199, 245
BssECI CCNNGG 2 cut(s) 434, 867
BssMI GATC 6 cut(s) 109, 271, 277, 422, 625, 727
BssNI GRCGYC 1 cut(s) 593
Bst2UI CCWGG 2 cut(s) 435, 1086
Bst4CI ACNGT 4 cut(s) 192, 418, 562, 712
Bst6I CTCTTC 2 cut(s) 525, 1151
BstACI GRCGYC 1 cut(s) 593
BstAPI GCANNNNNTGC 1 cut(s) 59
BstAUI TGTACA 1 cut(s) 618
BstC8I GCNNGC 3 cut(s) 294, 957, 1098
BstF5I GGATG 2 cut(s) 27, 141
BstHHI GCGC 1 cut(s) 1174
BstKTI GATC 6 cut(s) 112, 274, 280, 425, 628, 730
BstMAI GTCTC 3 cut(s) 48, 460, 673
BstMBI GATC 6 cut(s) 109, 271, 277, 422, 625, 727
BstMWI GCNNNNNNNGC 5 cut(s) 32, 59, 821, 1064, 1109
BstNI CCWGG 2 cut(s) 435, 1086
BstNSI RCATGY 3 cut(s) 296, 473, 619
BstSCI CCNGG 4 cut(s) 433, 866, 950, 1084
BstSFI CTRYAG 1 cut(s) 762
BstSLI GKGCMC 1 cut(s) 811
BstV1I GCAGC 1 cut(s) 745
BstV2I GAAGAC 1 cut(s) 374
BstX2I RGATCY 1 cut(s) 625
BstXI CCANNNNNNTGG 1 cut(s) 257
BstYI RGATCY 1 cut(s) 625
Bsu36I CCTNAGG 1 cut(s) 684
BsuRI GGCC 1 cut(s) 1001
BtsCI GGATG 2 cut(s) 27, 141
BtsIMutI CAGTG 1 cut(s) 394
BveI ACCTGC 3 cut(s) 44, 1132, 1177
Cac8I GCNNGC 3 cut(s) 294, 957, 1098
CciI TCATGA 1 cut(s) 274
CfoI GCGC 1 cut(s) 1174
Cfr13I GGNCC 2 cut(s) 100, 999
Csp6I GTAC 1 cut(s) 619
CviAII CATG 5 cut(s) 275, 293, 470, 509, 616
CviQI GTAC 1 cut(s) 619
DpnI GATC 6 cut(s) 111, 273, 279, 424, 627, 729
DpnII GATC 6 cut(s) 109, 271, 277, 422, 625, 727
Eam1104I CTCTTC 2 cut(s) 525, 1151
EarI CTCTTC 2 cut(s) 525, 1151
Eco31I GGTCTC 1 cut(s) 673
Eco47I GGWCC 1 cut(s) 100
Eco57I CTGAAG 3 cut(s) 104, 549, 777
Eco81I CCTNAGG 1 cut(s) 684
EcoO109I RGGNCCY 1 cut(s) 100
EcoRII CCWGG 2 cut(s) 433, 1084
EcoT22I ATGCAT 2 cut(s) 294, 617
FaeI CATG 5 cut(s) 278, 296, 473, 512, 619
FatI CATG 5 cut(s) 274, 292, 469, 508, 615
FblI GTMKAC 1 cut(s) 552
Fnu4HI GCNGC 2 cut(s) 734, 1101
FokI GGATG 2 cut(s) 14, 128
Fsp4HI GCNGC 2 cut(s) 734, 1101
FspBI CTAG 4 cut(s) 32, 222, 629, 1008
GlaI GCGC 1 cut(s) 1173
GluI GCNGC 2 cut(s) 734, 1101
GsaI CCCAGC 1 cut(s) 285
GsuI CTGGAG 2 cut(s) 821, 1068
HaeIII GGCC 1 cut(s) 1001
HapII CCGG 3 cut(s) 867, 951, 1062
HhaI GCGC 1 cut(s) 1174
Hin1I GRCGYC 1 cut(s) 593
Hin1II CATG 5 cut(s) 278, 296, 473, 512, 619
Hin6I GCGC 1 cut(s) 1172
HinP1I GCGC 1 cut(s) 1172
HinfI GANTC 9 cut(s) 41, 209, 214, 335, 356, 569, 975, 1091, 1162
HpaII CCGG 3 cut(s) 867, 951, 1062
HphI GGTGA 4 cut(s) 423, 451, 671, 1100
Hpy166II GTNNAC 3 cut(s) 250, 553, 809
Hpy188I TCNGA 5 cut(s) 154, 304, 487, 879, 1136
Hpy188III TCNNGA 6 cut(s) 17, 275, 401, 566, 800, 972
Hpy8I GTNNAC 3 cut(s) 250, 553, 809
HpyAV CCTTC 3 cut(s) 71, 108, 1040
HpyCH4III ACNGT 4 cut(s) 192, 418, 562, 712
HpyCH4IV ACGT 1 cut(s) 593
HpyCH4V TGCA 8 cut(s) 62, 74, 182, 292, 615, 655, 809, 994
HpyF10VI GCNNNNNNNGC 5 cut(s) 32, 59, 821, 1064, 1109
HpySE526I ACGT 1 cut(s) 593
Hsp92I GRCGYC 1 cut(s) 593
Hsp92II CATG 5 cut(s) 278, 296, 473, 512, 619
HspAI GCGC 1 cut(s) 1172
Kzo9I GATC 6 cut(s) 109, 271, 277, 422, 625, 727
LmnI GCTCC 1 cut(s) 583
Lsp1109I GCAGC 1 cut(s) 745
LweI GCATC 2 cut(s) 147, 437
MaeI CTAG 4 cut(s) 32, 222, 629, 1008
MaeII ACGT 1 cut(s) 593
MalI GATC 6 cut(s) 111, 273, 279, 424, 627, 729
MbiI CCGCTC 1 cut(s) 578
MboI GATC 6 cut(s) 109, 271, 277, 422, 625, 727
MboII GAAGA 6 cut(s) 97, 119, 374, 454, 542, 1168
MflI RGATCY 1 cut(s) 625
MhlI GDGCHC 1 cut(s) 811
MluCI AATT 4 cut(s) 345, 542, 837, 883
MlyI GAGTC 3 cut(s) 50, 350, 969
MmeI TCCRAC 1 cut(s) 493
MnlI CCTC 9 cut(s) 155, 526, 598, 643, 679, 715, 763, 796, 1152
Mph1103I ATGCAT 2 cut(s) 294, 617
MseI TTAA 1 cut(s) 903
MslI CAYNNNNRTG 1 cut(s) 474
MspA1I CMGCKG 1 cut(s) 736
MspI CCGG 3 cut(s) 867, 951, 1062
MspR9I CCNGG 4 cut(s) 435, 868, 952, 1086
MvaI CCWGG 2 cut(s) 435, 1086
MwoI GCNNNNNNNGC 5 cut(s) 32, 59, 821, 1064, 1109
NciI CCSGG 2 cut(s) 868, 952
NdeII GATC 6 cut(s) 109, 271, 277, 422, 625, 727
NlaIII CATG 5 cut(s) 278, 296, 473, 512, 619
NlaIV GGNNCC 1 cut(s) 319
NsiI ATGCAT 2 cut(s) 294, 617
NspI RCATGY 3 cut(s) 296, 473, 619
PaeI GCATGC 1 cut(s) 296
PagI TCATGA 1 cut(s) 274
PaqCI CACCTGC 2 cut(s) 44, 1132
PciI ACATGT 1 cut(s) 469
PcsI WCGNNNNNNNCGW 1 cut(s) 952
PfeI GAWTC 6 cut(s) 209, 214, 335, 569, 1091, 1162
PflFI GACNNNGTC 1 cut(s) 1181
PkrI GCNGC 2 cut(s) 735, 1102
PleI GAGTC 3 cut(s) 49, 350, 969
PpsI GAGTC 3 cut(s) 49, 350, 969
PpuMI RGGWCCY 1 cut(s) 100
PscI ACATGT 1 cut(s) 469
Psp5II RGGWCCY 1 cut(s) 100
Psp6I CCWGG 2 cut(s) 433, 1084
PspFI CCCAGC 1 cut(s) 281
PspGI CCWGG 2 cut(s) 433, 1084
PspN4I GGNNCC 1 cut(s) 319
PspPI GGNCC 2 cut(s) 100, 999
PspPPI RGGWCCY 1 cut(s) 100
PsuI RGATCY 1 cut(s) 625
PsyI GACNNNGTC 1 cut(s) 1181
RsaI GTAC 1 cut(s) 620
RsaNI GTAC 1 cut(s) 619
RseI CAYNNNNRTG 1 cut(s) 474
SaqAI TTAA 1 cut(s) 903
SatI GCNGC 2 cut(s) 734, 1101
Sau3AI GATC 6 cut(s) 109, 271, 277, 422, 625, 727
Sau96I GGNCC 2 cut(s) 100, 999
SchI GAGTC 3 cut(s) 50, 350, 969
ScrFI CCNGG 4 cut(s) 435, 868, 952, 1086
SduI GDGCHC 1 cut(s) 811
SfaNI GCATC 2 cut(s) 147, 437
SfcI CTRYAG 1 cut(s) 762
SinI GGWCC 1 cut(s) 100
SmiMI CAYNNNNRTG 1 cut(s) 474
SphI GCATGC 1 cut(s) 296
Sse9I AATT 4 cut(s) 345, 542, 837, 883
SsiI CCGC 3 cut(s) 576, 736, 1101
SspI AATATT 1 cut(s) 747
SspMI CTAG 4 cut(s) 32, 222, 629, 1008
StyD4I CCNGG 4 cut(s) 433, 866, 950, 1084
TaaI ACNGT 4 cut(s) 192, 418, 562, 712
TaiI ACGT 1 cut(s) 596
TaqI TCGA 2 cut(s) 112, 212
TasI AATT 4 cut(s) 345, 542, 837, 883
TatI WGTACW 1 cut(s) 618
TauI GCSGC 1 cut(s) 1103
TfiI GAWTC 6 cut(s) 209, 214, 335, 569, 1091, 1162
Tru1I TTAA 1 cut(s) 903
Tru9I TTAA 1 cut(s) 903
TscAI CASTG 1 cut(s) 394
TseI GCWGC 1 cut(s) 733
TspDTI ATGAA 4 cut(s) 319, 497, 947, 1059
TspGWI ACGGA 1 cut(s) 844
TspRI CASTG 1 cut(s) 394
Tth111I GACNNNGTC 1 cut(s) 1181
VneI GTGCAC 1 cut(s) 807
VpaK11BI GGWCC 1 cut(s) 100
XapI RAATTY 1 cut(s) 837
XceI RCATGY 3 cut(s) 296, 473, 619
XmiI GTMKAC 1 cut(s) 552
XspI CTAG 4 cut(s) 32, 222, 629, 1008
ZraI GACGTC 1 cut(s) 594
Zsp2I ATGCAT 2 cut(s) 294, 617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.