Rroxscaffold_1G00074130

VWA / Hh protein intein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000360
Physical Location & Seq
Forward (+)
95069219 .. 95073523
4305 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00074130.1

Sequence Viewer

Length: 2100 bp
ATGAATTCGTCTGAGGCTGCATCTGATCAAGTCAGTAACTTTGTGATATTTTGGGTCAAAGTTTTGGTAGCTATTAGTAATATTGTTAAAGAACCAATCTTTTCTCCCACACTTCCCCAGAGAGATTATGAATTCTATAGGAGTAGCCTGACAAGAGGGCTAAGCGAAGCCATCTTCACTGCCGAGTGCTCGCACGTTTTCCACTACCATTGCATTCTCAAAAATGTCCAGCATGGAAATCTTTGCTGCCCTGTCTGCCAAGCAAAGTGGAATAAGGATAATGTACCTTTCCAGGTTCCCCCTCCTCAGAACAACAACTTGGGCACAATCCCTTGTCACTTCACGTTCCCATCACAGCAGCCACACCATTGGTATTATCCTCAGTACCCATCCATGCCAGAACACGAGTACCCTCCTGAGCCACCTACCTTCTCTGACGATGAACCTCTCCCATCGTGCACCTCATCCATTCAATCCTCTGCCCCTCAGAATATAACAGTCAAGACTCATACAGAGTCCTCTGCTATCTCTTCTGCAGAATCAATCCCAAATTTTCCCGTCCTTGTCAGCATTCTTGCACCGCCCCTTCAGGATTCTGAGGGACATGGCCGTACACCCATTGACCTTGTAACAGTTCTGGACGTAAGTGGCAGCATGGAAGGCACAAAGCTTTCCCTTCTCAAGCAAGCTGTCACATTTGTCATAGAAAACTTGGGGCCTTCGGATAGACTTTCTGTAGTTTCGTTCTCATCAAGTTGTAGAAGAGTCTTTCCTCTCCAGAGAATGTCTGTTGATGGCCATGAAAGTGCCATCCTAGCCATTGAGTCTCTTAGAGCAGGTGGTGCAACTAACATTGCTGAAGGACTCAAGAAAGGAATTAAGGTCCTTGAAGATCGAAGGGAAAGAAACCCAGTTTCCAGTATCATCCTCTTATCTGATGGCATAGATACATACAATCGAACTGCAAACCGACTGTTAAACCAGTTGCCTGATTCGATTCGTTCTAGTAACATGCAACATGAAATCCCAGTCCACACATTTGGATTTGGCTACGACCATGATCCCAACATTATGCATGCTATATCTGATGTGTCAGGTGGCACCTTTTCGTTCATTGAATCAGTTGGAATGATACAAGACTCCTTTGCTCTGTGTATTGGTGGTCTTCTCAGTGTTGTGGCTCAGGAAGTTCGCCTAACAGTAAGGTCAGCATCGCCTGGGGTGAAGATTTTAGCAATACCATCAGGGAGACACGTGAATGAGATTTCTGATGAGGGTCAACAAGGTGTTGTTCAAGTTGGAAATATGTATGCAGAAGAGGAGAAACAATTTCTGGTCTACCTCTTAGTTCCAGAATCCTCAGGTCCGTATACTAAGATGTCATTGTTAGATGTGTTATGCATGTACAGAGATCTAGCTTCCAATGAACTCATCCAGGTGCAAGGTGAGAAAATAGAGATACTGAGACCCGAGGTTTGTTCCCCTGCTGAAAAGGCAGTGTCTTTGGAGGTTGATCGGCAGCGGAACCGAATTTTGGTGGCTGAAGCTATTGCTGAGGCACAAAGGTTGGCTGAGATGGGAAATATTGAGGGTGCACAGGTTCTATTGGCTCAACGGAAAGAAATTCTCTTAACATCTCCAGCATCCCAAGCTGGGGATTATCACAGTAATTTGTTTGAAACTGAGCTTAGAGAAATCATGGATAGAATGGCATCTATGAAGTTGTATAAACAAACCGGGCGTGCTTACGCTCTTGCAGGAATCAGTTCCCATAAATCACAAAGGGCCAGTGCTAGGGGTGACACAAGAGCCTCCTGTCTCAATTCGGGTTCAGTTGTCTCCAGGAAGAGGGGTCTCCGTAGTCGCAACAGTACCTACAGTTTTGCTCCAGGTGAGTTGGGTCAACCACCTGCCGCAGTTGGTGCTTTCGAAACACCTGCTATGGTCAGAATGGTGTGTACAGTTAACAAATTGGCAATTAAGGCTTTATTTGTGGATTATTTTATAGATATTATTGTTATTGTAACCAGCGATGACTTCTATGGTAATGTTGATGTCTTGGAGCTCAAGGAAACCCAATGGCTCCCTATTCCCCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VWA Vwaint zf-RING_11 zf-RING_2

Protein Analysis

699

Amino Acids

76.91

Weight (kDa)

5.63

Isoelectric Point (pI)

59.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_2 PF13923 54 - 86 6.4e-06 Zinc finger, C3HC4 type (RING finger)
VWA_CoxE PF05762 204 - 329 4e-08 VWA domain containing CoxE-like protein
VWA PF00092 208 - 374 6.4e-22 von Willebrand factor type A domain
VWA_3 PF13768 208 - 371 3.6e-18 von Willebrand factor type A domain
VWA_2 PF13519 209 - 314 2.2e-18 von Willebrand factor type A domain
WAV3_C PF25243 504 - 571 3e-06 E3 ubiquitin-protein ligase WAV3-like, C-terminal domain
Vwaint PF14624 560 - 603 1.8e-09 VWA / Hh protein intein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000360)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11691 FvH4_3g11693 FvH4_3g11740 FvH4_3g11750 FvH4_3g11770 FvH4_3g11771 FvH4_3g11772 FvH4_3g11780
malus_domestica MD10G1239000.v1.1
prunus_persica Prupe.4G105400_v2.0.a1
pyrus_communis pycom10g20000
rosa_chinensis RchiOBHm_Chr5g0018981 RchiOBHm_Chr5g0018991 RchiOBHm_Chr5g0019101 RchiOBHm_Chr5g0019111 RchiOBHm_Chr5g0019121 RchiOBHm_Chr5g0019151 RchiOBHm_Chr5g0019161 RchiOBHm_Chr5g0019171 RchiOBHm_Chr5g0019181 RchiOBHm_Chr5g0019201
rosa_laevigata RLG00000032411 RLG00000032418 RLG00000032419 RLG00000032420 RLG00000032421 RLG00000032423 RLG00000032424 RLG00000032426
rosa_multiflora Rmu_co8475613.1_g000001 Rmu_co8503587.1_g000001 Rmu_sc0000563.1_g000023 Rmu_sc0000563.1_g000027 Rmu_sc0002142.1_g000001 Rmu_sc0002142.1_g000003 Rmu_sc0002142.1_g000004 Rmu_sc0002142.1_g000011 Rmu_sc0002142.1_g000022 Rmu_sc0002561.1_g000002 Rmu_sc0042836.1_g000001
rosa_roxburghii Rroxscaffold_1G00058790 Rroxscaffold_1G00058810 Rroxscaffold_1G00058820 Rroxscaffold_1G00058840 Rroxscaffold_1G00058850 Rroxscaffold_1G00058860 Rroxscaffold_1G00058880 Rroxscaffold_1G00058910 Rroxscaffold_1G00074090 Rroxscaffold_1G00074110 Rroxscaffold_1G00074120 Rroxscaffold_1G00074130 Rroxscaffold_1G00074150
rosa_rugosa Rorug05G0046800 Rorug05G0047200 Rorug05G0047300 Rorug05G0047400 Rorug05G0047600 Rorug05G0047600 Rorug05G0047800 Rorug05G0047900 Rorug05G0048000 Rorug05G0048100 Rorug05G0048100 Rorug05G0048200 Rorug05G0048300
rosa_samantha Rh4DG102800 Rh5AG138700 Rh5AG138900 Rh5AG139000 Rh5AG139100 Rh5AG139200 Rh5AG139400 Rh5AG139500 Rh5AG139600 Rh5BG137400 Rh5BG137600 Rh5BG137800 Rh5BG138100 Rh5BG138200 Rh5BG138300 Rh5BG138500 Rh5CG148800 Rh5CG148900 Rh5CG149200 Rh5CG149300 Rh5CG149400 Rh5CG149600 Rh5CG149700 Rh5CG150000 Rh5DG138200 Rh5DG138300 Rh5DG138500 Rh5DG138600 Rh5DG138700 Rh5DG138900 Rh5DG139000 Rh5DG139200
rosa_wichuraiana Rw5G012290 Rw5G012330 Rw5G012340 Rw5G012350 Rw5G012370 Rw5G012380 Rw5G012390 Rw5G012410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 827, 1918, 1945
Acc36I ACCTGC 3 cut(s) 827, 1918, 1945
AccB1I GGYRCC 1 cut(s) 1100
AccI GTMKAC 2 cut(s) 1338, 1370
AciI CCGC 3 cut(s) 581, 1522, 1914
AclWI GGATC 1 cut(s) 1055
AcoI YGGCCR 2 cut(s) 607, 796
AcsI RAATTY 5 cut(s) 4, 131, 550, 1530, 1623
AcuI CTGAAG 3 cut(s) 572, 879, 1563
AcvI CACGTG 1 cut(s) 1255
AfaI GTAC 7 cut(s) 285, 386, 410, 613, 1406, 1873, 1960
AfiI CCNNNNNNNGG 5 cut(s) 1534, 1653, 1654, 1794, 1848
AflIII ACRYGT 1 cut(s) 1252
AgsI TTSAA 5 cut(s) 473, 890, 1118, 1295, 1679
AjnI CCWGG 5 cut(s) 291, 1216, 1434, 1841, 1888
AjuI GAANNNNNNNTTGG 4 cut(s) 302, 334, 1517, 1549
AluBI AGCT 8 cut(s) 71, 670, 689, 1418, 1547, 1652, 1687, 2065
AluI AGCT 8 cut(s) 71, 670, 689, 1418, 1547, 1652, 1687, 2065
Alw21I GWGCWC 4 cut(s) 191, 461, 1597, 2067
Alw26I GTCTC 6 cut(s) 831, 1243, 1459, 1823, 1843, 1859
Alw44I GTGCAC 2 cut(s) 457, 1593
AlwI GGATC 1 cut(s) 1055
Ama87I CYCGRG 1 cut(s) 1469
AoxI GGCC 4 cut(s) 607, 716, 796, 1785
ApaLI GTGCAC 2 cut(s) 457, 1593
ApeKI GCWGC 5 cut(s) 17, 246, 358, 651, 1519
ApoI RAATTY 5 cut(s) 4, 131, 550, 1530, 1623
ArsI GACNNNNNNTTYG 2 cut(s) 1458, 1490
Asp700I GAANNNNTTC 1 cut(s) 1765
AspS9I GGNCC 4 cut(s) 716, 883, 1364, 1785
AsuC2I CCSGG 1 cut(s) 1738
AsuHPI GGTGA 4 cut(s) 1234, 1457, 1811, 1904
AsuII TTCGAA 1 cut(s) 1929
AvaI CYCGRG 1 cut(s) 1469
AvaII GGWCC 2 cut(s) 883, 1364
AxyI CCTNAGG 1 cut(s) 1360
BaeGI GKGCMC 3 cut(s) 326, 461, 1597
BaeI ACNNNNGTAYC 2 cut(s) 392, 425
BalI TGGCCA 1 cut(s) 798
BanI GGYRCC 1 cut(s) 1100
BanII GRGCYC 1 cut(s) 2067
BauI CACGAG 1 cut(s) 404
BbrPI CACGTG 1 cut(s) 1255
BbsI GAAGAC 1 cut(s) 1157
Bbv12I GWGCWC 4 cut(s) 191, 461, 1597, 2067
BbvCI CCTCAGC 1 cut(s) 1554
BbvI GCAGC 5 cut(s) 4, 233, 370, 663, 1531
BccI CCATC 9 cut(s) 179, 358, 397, 460, 788, 818, 932, 1249, 1570
BceAI ACGGC 1 cut(s) 594
BcgI CGANNNNNNTGC 2 cut(s) 1919, 1953
BciT130I CCWGG 5 cut(s) 293, 1218, 1436, 1843, 1890
BclI TGATCA 1 cut(s) 25
BcnI CCSGG 1 cut(s) 1738
BcoDI GTCTC 6 cut(s) 831, 1243, 1459, 1823, 1843, 1859
BfaI CTAG 4 cut(s) 815, 1005, 1415, 1794
BfmI CTRYAG 4 cut(s) 136, 534, 735, 1876
BfuAI ACCTGC 3 cut(s) 827, 1918, 1945
BglII AGATCT 1 cut(s) 1411
BisI GCNGC 6 cut(s) 18, 247, 359, 652, 1520, 1914
BlpI GCTNAGC 1 cut(s) 161
BlsI GCNGC 6 cut(s) 19, 248, 360, 653, 1521, 1915
Bme1390I CCNGG 6 cut(s) 293, 1218, 1436, 1738, 1843, 1890
Bme18I GGWCC 2 cut(s) 883, 1364
BmeT110I CYCGRG 1 cut(s) 1469
BmgT120I GGNCC 4 cut(s) 716, 883, 1364, 1785
BmiI GGNNCC 5 cut(s) 297, 717, 1102, 1526, 2084
BmrFI CCNGG 6 cut(s) 293, 1218, 1436, 1738, 1843, 1890
BmrI ACTGGG 2 cut(s) 905, 1022
BmsI GCATC 4 cut(s) 29, 1220, 1652, 1721
BmuI ACTGGG 2 cut(s) 905, 1022
BpiI GAAGAC 1 cut(s) 1157
BpmI CTGGAG 4 cut(s) 761, 1623, 1825, 1872
Bpu10I CCTNAGC 3 cut(s) 417, 1182, 1554
Bpu1102I GCTNAGC 1 cut(s) 161
Bpu14I TTCGAA 1 cut(s) 1929
BpuEI CTTGAG 3 cut(s) 665, 851, 2051
BpuMI CCSGG 1 cut(s) 1738
BsaAI YACGTR 1 cut(s) 1255
BsaI GGTCTC 2 cut(s) 1459, 1859
BsaJI CCNNGG 2 cut(s) 1217, 1470
Bsc4I CCNNNNNNNGG 5 cut(s) 1534, 1653, 1654, 1794, 1848
Bse1I ACTGG 5 cut(s) 911, 918, 982, 1028, 1788
Bse21I CCTNAGG 1 cut(s) 1360
Bse3DI GCAATG 2 cut(s) 208, 852
BseBI CCWGG 5 cut(s) 293, 1218, 1436, 1843, 1890
BseDI CCNNGG 2 cut(s) 1217, 1470
BseGI GGATG 6 cut(s) 389, 464, 810, 924, 1431, 1643
BseLI CCNNNNNNNGG 5 cut(s) 1534, 1653, 1654, 1794, 1848
BseMI GCAATG 2 cut(s) 208, 852
BseNI ACTGG 5 cut(s) 911, 918, 982, 1028, 1788
BseRI GAGGAG 2 cut(s) 294, 1334
BseSI GKGCMC 3 cut(s) 326, 461, 1597
BseXI GCAGC 5 cut(s) 4, 233, 370, 663, 1531
BseYI CCCAGC 1 cut(s) 1652
BshFI GGCC 4 cut(s) 609, 718, 798, 1787
BshNI GGYRCC 1 cut(s) 1100
BsiHKAI GWGCWC 4 cut(s) 191, 461, 1597, 2067
BsiHKCI CYCGRG 1 cut(s) 1469
BsiSI CCGG 1 cut(s) 1737
BslFI GGGAC 1 cut(s) 615
BslI CCNNNNNNNGG 5 cut(s) 1534, 1653, 1654, 1794, 1848
BsmAI GTCTC 6 cut(s) 831, 1243, 1459, 1823, 1843, 1859
BsmFI GGGAC 1 cut(s) 615
BsmI GAATGC 2 cut(s) 213, 570
BsnI GGCC 4 cut(s) 609, 718, 798, 1787
Bso31I GGTCTC 2 cut(s) 1459, 1859
BsoBI CYCGRG 1 cut(s) 1469
Bsp119I TTCGAA 1 cut(s) 1929
Bsp1286I GDGCHC 5 cut(s) 191, 326, 461, 1597, 2067
Bsp1407I TGTACA 2 cut(s) 1404, 1958
Bsp143I GATC 5 cut(s) 25, 892, 1060, 1411, 1513
Bsp1720I GCTNAGC 1 cut(s) 161
BspACI CCGC 3 cut(s) 581, 1522, 1914
BspANI GGCC 4 cut(s) 609, 718, 798, 1787
BspLI GGNNCC 5 cut(s) 297, 717, 1102, 1526, 2084
BspMAI CTGCAG 1 cut(s) 538
BspMI ACCTGC 3 cut(s) 827, 1918, 1945
BspPI GGATC 1 cut(s) 1055
BspT104I TTCGAA 1 cut(s) 1929
BspT107I GGYRCC 1 cut(s) 1100
BspTNI GGTCTC 2 cut(s) 1459, 1859
BsrDI GCAATG 2 cut(s) 208, 852
BsrGI TGTACA 2 cut(s) 1404, 1958
BsrI ACTGG 5 cut(s) 911, 918, 982, 1028, 1788
BssECI CCNNGG 2 cut(s) 1217, 1470
BssMI GATC 5 cut(s) 25, 892, 1060, 1411, 1513
BssNAI GTATAC 1 cut(s) 1371
BssSI CACGAG 1 cut(s) 404
Bst1107I GTATAC 1 cut(s) 1371
Bst2BI CACGAG 1 cut(s) 404
Bst2UI CCWGG 5 cut(s) 293, 1218, 1436, 1843, 1890
Bst4CI ACNGT 8 cut(s) 499, 634, 975, 1201, 1667, 1871, 1880, 1963
Bst6I CTCTTC 4 cut(s) 535, 757, 1311, 1841
BstAPI GCANNNNNTGC 2 cut(s) 842, 1922
BstAUI TGTACA 2 cut(s) 1404, 1958
BstBAI YACGTR 1 cut(s) 1255
BstBI TTCGAA 1 cut(s) 1929
BstC8I GCNNGC 4 cut(s) 191, 687, 1077, 1743
BstF5I GGATG 6 cut(s) 389, 464, 810, 924, 1431, 1643
BstKTI GATC 5 cut(s) 28, 895, 1063, 1414, 1516
BstMAI GTCTC 6 cut(s) 831, 1243, 1459, 1823, 1843, 1859
BstMBI GATC 5 cut(s) 25, 892, 1060, 1411, 1513
BstMWI GCNNNNNNNGC 8 cut(s) 255, 660, 815, 842, 1493, 1649, 1922, 1982
BstNI CCWGG 5 cut(s) 293, 1218, 1436, 1843, 1890
BstNSI RCATGY 3 cut(s) 1015, 1079, 1405
BstSCI CCNGG 6 cut(s) 291, 1216, 1434, 1736, 1841, 1888
BstSFI CTRYAG 4 cut(s) 136, 534, 735, 1876
BstSLI GKGCMC 3 cut(s) 326, 461, 1597
BstV1I GCAGC 5 cut(s) 4, 233, 370, 663, 1531
BstV2I GAAGAC 1 cut(s) 1157
BstX2I RGATCY 1 cut(s) 1411
BstXI CCANNNNNNTGG 2 cut(s) 369, 1040
BstYI RGATCY 1 cut(s) 1411
BstZ17I GTATAC 1 cut(s) 1371
Bsu36I CCTNAGG 1 cut(s) 1360
BsuRI GGCC 4 cut(s) 609, 718, 798, 1787
BtgZI GCGATG 2 cut(s) 1197, 2046
BtsCI GGATG 6 cut(s) 389, 464, 810, 924, 1431, 1643
BtsI GCAGTG 2 cut(s) 177, 1503
BtsIMutI CAGTG 4 cut(s) 177, 1177, 1503, 1795
BveI ACCTGC 3 cut(s) 827, 1918, 1945
Cac8I GCNNGC 4 cut(s) 191, 687, 1077, 1743
Cfr13I GGNCC 4 cut(s) 716, 883, 1364, 1785
Csp6I GTAC 7 cut(s) 284, 385, 409, 612, 1405, 1872, 1959
CspCI CAANNNNNGTGG 4 cut(s) 191, 226, 248, 283
CviQI GTAC 7 cut(s) 284, 385, 409, 612, 1405, 1872, 1959
DpnI GATC 5 cut(s) 27, 894, 1062, 1413, 1515
DpnII GATC 5 cut(s) 25, 892, 1060, 1411, 1513
EaeI YGGCCR 2 cut(s) 607, 796
Eam1104I CTCTTC 4 cut(s) 535, 757, 1311, 1841
EarI CTCTTC 4 cut(s) 535, 757, 1311, 1841
Ecl136II GAGCTC 1 cut(s) 2065
Eco24I GRGCYC 1 cut(s) 2067
Eco31I GGTCTC 2 cut(s) 1459, 1859
Eco47I GGWCC 2 cut(s) 883, 1364
Eco53kI GAGCTC 1 cut(s) 2065
Eco57I CTGAAG 3 cut(s) 572, 879, 1563
Eco72I CACGTG 1 cut(s) 1255
Eco81I CCTNAGG 1 cut(s) 1360
Eco88I CYCGRG 1 cut(s) 1469
EcoICRI GAGCTC 1 cut(s) 2065
EcoO109I RGGNCCY 2 cut(s) 716, 883
EcoRI GAATTC 2 cut(s) 4, 131
EcoRII CCWGG 5 cut(s) 291, 1216, 1434, 1841, 1888
EcoT22I ATGCAT 2 cut(s) 1077, 1403
EcoT38I GRGCYC 1 cut(s) 2067
FaqI GGGAC 1 cut(s) 615
FbaI TGATCA 1 cut(s) 25
FblI GTMKAC 2 cut(s) 1338, 1370
Fnu4HI GCNGC 6 cut(s) 18, 247, 359, 652, 1520, 1914
FokI GGATG 6 cut(s) 376, 451, 797, 911, 1418, 1630
FriOI GRGCYC 1 cut(s) 2067
Fsp4HI GCNGC 6 cut(s) 18, 247, 359, 652, 1520, 1914
FspBI CTAG 4 cut(s) 815, 1005, 1415, 1794
GluI GCNGC 6 cut(s) 18, 247, 359, 652, 1520, 1914
GsaI CCCAGC 1 cut(s) 1656
GsuI CTGGAG 4 cut(s) 761, 1623, 1825, 1872
HaeIII GGCC 4 cut(s) 609, 718, 798, 1787
HapII CCGG 1 cut(s) 1737
HincII GTYRAC 3 cut(s) 1280, 1904, 1966
HindII GTYRAC 3 cut(s) 1280, 1904, 1966
HindIII AAGCTT 1 cut(s) 668
HpaI GTTAAC 1 cut(s) 1966
HpaII CCGG 1 cut(s) 1737
HphI GGTGA 4 cut(s) 1234, 1457, 1811, 1904
Hpy188III TCNNGA 8 cut(s) 416, 502, 590, 638, 778, 868, 1184, 1352
HpyAV CCTTC 7 cut(s) 439, 596, 653, 686, 729, 854, 891
HpyCH4III ACNGT 8 cut(s) 499, 634, 975, 1201, 1667, 1871, 1880, 1963
HpyCH4IV ACGT 4 cut(s) 195, 344, 642, 1254
HpyF10VI GCNNNNNNNGC 8 cut(s) 255, 660, 815, 842, 1493, 1649, 1922, 1982
HpySE526I ACGT 4 cut(s) 195, 344, 642, 1254
Ksp22I TGATCA 1 cut(s) 25
KspAI GTTAAC 1 cut(s) 1966
Kzo9I GATC 5 cut(s) 25, 892, 1060, 1411, 1513
LmnI GCTCC 3 cut(s) 1891, 2062, 2088
Lsp1109I GCAGC 5 cut(s) 4, 233, 370, 663, 1531
LweI GCATC 4 cut(s) 29, 1220, 1652, 1721
MaeI CTAG 4 cut(s) 815, 1005, 1415, 1794
MaeII ACGT 4 cut(s) 195, 344, 642, 1254
MaeIII GTNAC 7 cut(s) 35, 335, 628, 691, 1007, 1799, 2023
MalI GATC 5 cut(s) 27, 894, 1062, 1413, 1515
MboI GATC 5 cut(s) 25, 892, 1060, 1411, 1513
MboII GAAGA 8 cut(s) 166, 522, 774, 902, 1157, 1237, 1328, 1858
MflI RGATCY 1 cut(s) 1411
MhlI GDGCHC 5 cut(s) 191, 326, 461, 1597, 2067
MlsI TGGCCA 1 cut(s) 798
MluNI TGGCCA 1 cut(s) 798
MlyI GAGTC 6 cut(s) 499, 524, 774, 833, 858, 1133
MmeI TCCRAC 2 cut(s) 1105, 1279
Mox20I TGGCCA 1 cut(s) 798
Mph1103I ATGCAT 2 cut(s) 1077, 1403
MroXI GAANNNNTTC 1 cut(s) 1765
MscI TGGCCA 1 cut(s) 798
MseI TTAA 6 cut(s) 87, 879, 977, 1631, 1965, 1980
MslI CAYNNNNRTG 3 cut(s) 804, 1257, 1436
Msp20I TGGCCA 1 cut(s) 798
MspA1I CMGCKG 1 cut(s) 1522
MspI CCGG 1 cut(s) 1737
MspR9I CCNGG 6 cut(s) 293, 1218, 1436, 1738, 1843, 1890
Mva1269I GAATGC 2 cut(s) 213, 570
MvaI CCWGG 5 cut(s) 293, 1218, 1436, 1843, 1890
MwoI GCNNNNNNNGC 8 cut(s) 255, 660, 815, 842, 1493, 1649, 1922, 1982
NciI CCSGG 1 cut(s) 1738
NdeII GATC 5 cut(s) 25, 892, 1060, 1411, 1513
NlaIV GGNNCC 5 cut(s) 297, 717, 1102, 1526, 2084
NmeAIII GCCGAG 1 cut(s) 208
NmuCI GTSAC 3 cut(s) 335, 691, 1799
NsiI ATGCAT 2 cut(s) 1077, 1403
NspI RCATGY 3 cut(s) 1015, 1079, 1405
NspV TTCGAA 1 cut(s) 1929
PaeI GCATGC 1 cut(s) 1079
PaqCI CACCTGC 3 cut(s) 827, 1918, 1945
PctI GAATGC 2 cut(s) 213, 570
PdmI GAANNNNTTC 1 cut(s) 1765
PfeI GAWTC 7 cut(s) 539, 593, 992, 997, 1118, 1355, 1761
PfoI TCCNGGA 1 cut(s) 1841
PkrI GCNGC 6 cut(s) 19, 248, 360, 653, 1521, 1915
PleI GAGTC 6 cut(s) 499, 523, 773, 832, 858, 1133
PmaCI CACGTG 1 cut(s) 1255
PmlI CACGTG 1 cut(s) 1255
PpsI GAGTC 6 cut(s) 499, 523, 773, 832, 858, 1133
Ppu21I YACGTR 1 cut(s) 1255
PpuMI RGGWCCY 1 cut(s) 883
Psp124BI GAGCTC 1 cut(s) 2067
Psp5II RGGWCCY 1 cut(s) 883
Psp6I CCWGG 5 cut(s) 291, 1216, 1434, 1841, 1888
PspCI CACGTG 1 cut(s) 1255
PspFI CCCAGC 1 cut(s) 1652
PspGI CCWGG 5 cut(s) 291, 1216, 1434, 1841, 1888
PspN4I GGNNCC 5 cut(s) 297, 717, 1102, 1526, 2084
PspPI GGNCC 4 cut(s) 716, 883, 1364, 1785
PspPPI RGGWCCY 1 cut(s) 883
PstI CTGCAG 1 cut(s) 538
PsuI RGATCY 1 cut(s) 1411
RsaI GTAC 7 cut(s) 285, 386, 410, 613, 1406, 1873, 1960
RsaNI GTAC 7 cut(s) 284, 385, 409, 612, 1405, 1872, 1959
RseI CAYNNNNRTG 3 cut(s) 804, 1257, 1436
SacI GAGCTC 1 cut(s) 2067
SaqAI TTAA 6 cut(s) 87, 879, 977, 1631, 1965, 1980
SatI GCNGC 6 cut(s) 18, 247, 359, 652, 1520, 1914
Sau3AI GATC 5 cut(s) 25, 892, 1060, 1411, 1513
Sau96I GGNCC 4 cut(s) 716, 883, 1364, 1785
SchI GAGTC 6 cut(s) 499, 524, 774, 833, 858, 1133
ScrFI CCNGG 6 cut(s) 293, 1218, 1436, 1738, 1843, 1890
SduI GDGCHC 5 cut(s) 191, 326, 461, 1597, 2067
SfaNI GCATC 4 cut(s) 29, 1220, 1652, 1721
SfcI CTRYAG 4 cut(s) 136, 534, 735, 1876
SfuI TTCGAA 1 cut(s) 1929
SinI GGWCC 2 cut(s) 883, 1364
SmiMI CAYNNNNRTG 3 cut(s) 804, 1257, 1436
SmlI CTYRAG 3 cut(s) 680, 866, 2066
SmoI CTYRAG 3 cut(s) 680, 866, 2066
SphI GCATGC 1 cut(s) 1079
SsiI CCGC 3 cut(s) 581, 1522, 1914
SspI AATATT 2 cut(s) 82, 1585
SspMI CTAG 4 cut(s) 815, 1005, 1415, 1794
SstI GAGCTC 1 cut(s) 2067
StyD4I CCNGG 6 cut(s) 291, 1216, 1434, 1736, 1841, 1888
TaaI ACNGT 8 cut(s) 499, 634, 975, 1201, 1667, 1871, 1880, 1963
TaiI ACGT 4 cut(s) 198, 347, 645, 1257
TaqI TCGA 4 cut(s) 895, 958, 995, 1929
TatI WGTACW 2 cut(s) 1404, 1958
TauI GCSGC 1 cut(s) 1916
TfiI GAWTC 7 cut(s) 539, 593, 992, 997, 1118, 1355, 1761
Tru1I TTAA 6 cut(s) 87, 879, 977, 1631, 1965, 1980
Tru9I TTAA 6 cut(s) 87, 879, 977, 1631, 1965, 1980
TscAI CASTG 4 cut(s) 184, 1177, 1503, 1795
TseFI GTSAC 3 cut(s) 335, 691, 1799
TseI GCWGC 5 cut(s) 17, 246, 358, 651, 1519
Tsp45I GTSAC 3 cut(s) 335, 691, 1799
TspDTI ATGAA 8 cut(s) 17, 144, 456, 816, 1035, 1102, 1440, 1733
TspGWI ACGGA 3 cut(s) 1356, 1630, 1847
TspRI CASTG 4 cut(s) 184, 1177, 1503, 1795
VneI GTGCAC 2 cut(s) 457, 1593
VpaK11BI GGWCC 2 cut(s) 883, 1364
XapI RAATTY 5 cut(s) 4, 131, 550, 1530, 1623
XceI RCATGY 3 cut(s) 1015, 1079, 1405
XmiI GTMKAC 2 cut(s) 1338, 1370
XmnI GAANNNNTTC 1 cut(s) 1765
XspI CTAG 4 cut(s) 815, 1005, 1415, 1794
Zsp2I ATGCAT 2 cut(s) 1077, 1403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.