Rh5DG138500

VWA / Hh protein intein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000360
Physical Location & Seq
Forward (+)
13939927 .. 13952247
12321 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG138500.1

Sequence Viewer

Length: 1701 bp
ATGTTCAAATTTTTAATTAGCTCGAAATTTGATTTGGATATCCAATGTAGTTGTAGTTTTGGCTCTTCTTGTAAGTCTTGCAAGTTAAACTTATTTTATTTCTCTTATAGTAACAGTGACAAAATGTCGAAACGTCTTCGTTCGAACGTTTCCGGTGCCCAAAAGGCTAATGTTGGTGTCACTTTGACAAAACGGCGACGTGTACAAGCAAACGAACCTTCACTTCCAAATTCTTTGCAAGATGAACTTGATGTTCCTGATATGCCTACCTCTAATAGAACTACCCAAAGGGCTATTGCTAGTACCATTATCCAAAGGCCTACTGTTGTTAGAATTTGTGCACCACCCCTTCAAGATCCCGATGGTGAAGGCCGTACACCCATTGACCTTGTTACAATTCTGGACGTAAGCGGTAGCATGCATGGCCAAAAGCTTGAACTTGTCAAGCAAGCTGTCAAATTCGTCATTGAAAACATGGGGACTTCGGACAGACTGTCTATAGTATCATTCTCAACAACTTCTAAACGACTCCTTCCTCTCCGTAGAATGACTGTTAAGGGCCGTGCAAGTGCAATCCAAGCTGTCGAATCTCTTAGAGCAGATGGTGGAACTGACATTGCAGAAGGACTCAAGATAGGAACTCATATCCTTGAGGAGCGAAGGGAAAGGAACCCTGTTGCTAGCATCATCCTCTTATCGGATGGCCAAGATAGCTACTGTGACAACCCAAGCCAAATGTTGAAGAAATTGCCTGCTTCAATTCGTTCTAGTGACATGCAACATGAAATCCCAGTCCACACATTTGGGTTTGGCAACGACCATAATGCAAAGATTATGCATACTATATCTAATGTATCAGGGGGAACGTTTTCGTACATTGAATCAGTTGGCATAATACAAGACGCTTTTGCTCTGTGCATTGGTGGTCTTCTCAGTGTTGTGGCTCAGGAACTTCGCCTCACGGTGAGGTCAGCATCACCTGGGGTGAAGATCGTGGAAATACCATCAGGGAGACATGTCAGTCAGATTTCTGATGAGCAAGGTGTTGTTGATGTTGGAAATATGTATGCAGAAGAGGAGAAACAGTTTCTGGTGTACCTCTCAGTTCCACAATCCGCAGCTCCGGATACCAAGACGTCTTTGGTAGACGTTTTATGCGTGTACAAAGATCTTGGTTCAGATGAGTTTATCCCGGTCCAAGGTGAGAAAGTAGAGATAACGAGACCTGATTTTTGTTCTCCTGCAGAAATGGCAGTGTCTTTGGAGGTTGATCGGCAGCGGAACAGACTTTTGGTGGCTGATGCTATTGGAGAGGCACAAAGGTTGGCTGAAATGGGAAATCTGGAGGCTGCACGGGCGGTTTTGACTCAGCGAAGAGAAACTCTCATGACATCAACAGCAGCCAAAGCTGGGGATGATCTGAGTCATATGTTTGAAACTGAGCTTAAACAAATCATGGATAAAATGGCAAGTATGAATTTGTATGAAGAGGTGGGGCGTGCTTACGCTCTCTCAGGGATGAGTTCCCATGCACGGCAGAGGGCCTCGGCTAGGGGTAACGCAGCAGGCTCGTGCCTGGCCGATAAGGCAGGTCGCAAACAACTTGCTACTAAAGCAGCAGGTCGTGCTACTTTTGAAACACCCGCTATGGTTAGAATGGTAAGAAAATCACAGAATAAGAGGAAGTCTGGTTCTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VWA Vwaint zf-RING_11 zf-RING_2

Protein Analysis

566

Amino Acids

61.76

Weight (kDa)

8.86

Isoelectric Point (pI)

50.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VWA_CoxE PF05762 125 - 243 2.3e-06 VWA domain containing CoxE-like protein
VWA PF00092 129 - 295 6.2e-23 von Willebrand factor type A domain
VWA_3 PF13768 129 - 290 4.3e-19 von Willebrand factor type A domain
VWA_2 PF13519 130 - 236 1.2e-18 von Willebrand factor type A domain
Vwaint PF14624 478 - 558 9.8e-12 VWA / Hh protein intein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000360)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11691 FvH4_3g11693 FvH4_3g11740 FvH4_3g11750 FvH4_3g11770 FvH4_3g11771 FvH4_3g11772 FvH4_3g11780
malus_domestica MD10G1239000.v1.1
prunus_persica Prupe.4G105400_v2.0.a1
pyrus_communis pycom10g20000
rosa_chinensis RchiOBHm_Chr5g0018981 RchiOBHm_Chr5g0018991 RchiOBHm_Chr5g0019101 RchiOBHm_Chr5g0019111 RchiOBHm_Chr5g0019121 RchiOBHm_Chr5g0019151 RchiOBHm_Chr5g0019161 RchiOBHm_Chr5g0019171 RchiOBHm_Chr5g0019181 RchiOBHm_Chr5g0019201
rosa_laevigata RLG00000032411 RLG00000032418 RLG00000032419 RLG00000032420 RLG00000032421 RLG00000032423 RLG00000032424 RLG00000032426
rosa_multiflora Rmu_co8475613.1_g000001 Rmu_co8503587.1_g000001 Rmu_sc0000563.1_g000023 Rmu_sc0000563.1_g000027 Rmu_sc0002142.1_g000001 Rmu_sc0002142.1_g000003 Rmu_sc0002142.1_g000004 Rmu_sc0002142.1_g000011 Rmu_sc0002142.1_g000022 Rmu_sc0002561.1_g000002 Rmu_sc0042836.1_g000001
rosa_roxburghii Rroxscaffold_1G00058790 Rroxscaffold_1G00058810 Rroxscaffold_1G00058820 Rroxscaffold_1G00058840 Rroxscaffold_1G00058850 Rroxscaffold_1G00058860 Rroxscaffold_1G00058880 Rroxscaffold_1G00058910 Rroxscaffold_1G00074090 Rroxscaffold_1G00074110 Rroxscaffold_1G00074120 Rroxscaffold_1G00074130 Rroxscaffold_1G00074150
rosa_rugosa Rorug05G0046800 Rorug05G0047200 Rorug05G0047300 Rorug05G0047400 Rorug05G0047600 Rorug05G0047600 Rorug05G0047800 Rorug05G0047900 Rorug05G0048000 Rorug05G0048100 Rorug05G0048100 Rorug05G0048200 Rorug05G0048300
rosa_samantha Rh4DG102800 Rh5AG138700 Rh5AG138900 Rh5AG139000 Rh5AG139100 Rh5AG139200 Rh5AG139400 Rh5AG139500 Rh5AG139600 Rh5BG137400 Rh5BG137600 Rh5BG137800 Rh5BG138100 Rh5BG138200 Rh5BG138300 Rh5BG138500 Rh5CG148800 Rh5CG148900 Rh5CG149200 Rh5CG149300 Rh5CG149400 Rh5CG149600 Rh5CG149700 Rh5CG150000 Rh5DG138200 Rh5DG138300 Rh5DG138500 Rh5DG138600 Rh5DG138700 Rh5DG138900 Rh5DG139000 Rh5DG139200
rosa_wichuraiana Rw5G012290 Rw5G012330 Rw5G012340 Rw5G012350 Rw5G012370 Rw5G012380 Rw5G012390 Rw5G012410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1020
AatII GACGTC 1 cut(s) 1139
Acc36I ACCTGC 2 cut(s) 1580, 1610
AccB1I GGYRCC 1 cut(s) 155
AccI GTMKAC 1 cut(s) 1146
AccIII TCCGGA 1 cut(s) 1123
AciI CCGC 5 cut(s) 411, 1116, 1279, 1358, 1644
AclI AACGTT 2 cut(s) 147, 866
AclWI GGATC 1 cut(s) 350
AcoI YGGCCR 3 cut(s) 424, 703, 1578
AcsI RAATTY 6 cut(s) 8, 26, 229, 333, 458, 1477
AcyI GRCGYC 1 cut(s) 1136
AfaI GTAC 6 cut(s) 204, 304, 376, 875, 1097, 1163
AfiI CCNNNNNNNGG 5 cut(s) 697, 1199, 1410, 1533, 1551
AflIII ACRYGT 2 cut(s) 199, 1015
AgsI TTSAA 9 cut(s) 7, 353, 437, 470, 742, 759, 881, 1436, 1637
AhdI GACNNNNNGTC 2 cut(s) 124, 493
AjiI CACGTC 1 cut(s) 200
AjnI CCWGG 2 cut(s) 979, 1575
AjuI GAANNNNNNNTTGG 4 cut(s) 17, 49, 1274, 1306
AloI GAACNNNNNNTCC 1 cut(s) 1675
AluBI AGCT 8 cut(s) 21, 433, 452, 581, 714, 1121, 1409, 1444
AluI AGCT 8 cut(s) 21, 433, 452, 581, 714, 1121, 1409, 1444
Alw21I GWGCWC 1 cut(s) 343
Alw26I GTCTC 2 cut(s) 1006, 1216
Alw44I GTGCAC 1 cut(s) 339
AlwI GGATC 1 cut(s) 350
AlwNI CAGNNNCTG 1 cut(s) 1090
Aor13HI TCCGGA 1 cut(s) 1123
AoxI GGCC 7 cut(s) 317, 370, 424, 559, 703, 1542, 1578
ApaLI GTGCAC 1 cut(s) 339
ApeKI GCWGC 6 cut(s) 1118, 1276, 1349, 1400, 1562, 1616
ApoI RAATTY 6 cut(s) 8, 26, 229, 333, 458, 1477
ArsI GACNNNNNNTTYG 2 cut(s) 1215, 1247
Asp700I GAANNNNTTC 1 cut(s) 868
AspS9I GGNCC 3 cut(s) 559, 1195, 1542
AsuC2I CCSGG 1 cut(s) 1193
AsuHPI GGTGA 5 cut(s) 377, 969, 976, 997, 1214
AsuII TTCGAA 1 cut(s) 143
AsuNHI GCTAGC 1 cut(s) 680
AvaII GGWCC 1 cut(s) 1195
BaeGI GKGCMC 2 cut(s) 160, 343
BalI TGGCCA 2 cut(s) 426, 705
BanI GGYRCC 1 cut(s) 155
BauI CACGAG 1 cut(s) 1570
BbsI GAAGAC 2 cut(s) 128, 920
Bbv12I GWGCWC 1 cut(s) 343
BbvI GCAGC 6 cut(s) 1130, 1288, 1336, 1412, 1574, 1628
BccI CCATC 4 cut(s) 356, 596, 695, 1012
BceAI ACGGC 4 cut(s) 209, 357, 546, 1550
BcgI CGANNNNNNTGC 4 cut(s) 806, 840, 1551, 1585
BciT130I CCWGG 2 cut(s) 981, 1577
BciVI GTATCC 1 cut(s) 1120
BcnI CCSGG 1 cut(s) 1193
BcoDI GTCTC 2 cut(s) 1006, 1216
BfaI CTAG 4 cut(s) 300, 681, 768, 1551
BfmI CTRYAG 2 cut(s) 498, 1242
BfuAI ACCTGC 2 cut(s) 1580, 1610
BfuI GTATCC 1 cut(s) 1120
BglI GCCNNNNNGGC 2 cut(s) 164, 1586
BglII AGATCT 1 cut(s) 1168
BisI GCNGC 6 cut(s) 1119, 1277, 1350, 1401, 1563, 1617
BlsI GCNGC 6 cut(s) 1120, 1278, 1351, 1402, 1564, 1618
Bme1390I CCNGG 3 cut(s) 981, 1193, 1577
Bme18I GGWCC 1 cut(s) 1195
BmeRI GACNNNNNGTC 2 cut(s) 124, 493
BmgBI CACGTC 1 cut(s) 200
BmgT120I GGNCC 3 cut(s) 559, 1195, 1542
BmiI GGNNCC 2 cut(s) 157, 671
BmrFI CCNGG 3 cut(s) 981, 1193, 1577
BmrI ACTGGG 1 cut(s) 785
BmsI GCATC 3 cut(s) 693, 983, 1291
BmtI GCTAGC 1 cut(s) 684
BmuI ACTGGG 1 cut(s) 785
BpiI GAAGAC 2 cut(s) 128, 920
BplI GAGNNNNNCTC 2 cut(s) 1368, 1400
BpmI CTGGAG 1 cut(s) 1364
Bpu10I CCTNAGC 1 cut(s) 945
Bpu14I TTCGAA 1 cut(s) 143
BpuEI CTTGAG 2 cut(s) 614, 671
BpuMI CCSGG 1 cut(s) 1193
BsaHI GRCGYC 1 cut(s) 1136
BsaI GGTCTC 1 cut(s) 1216
BsaJI CCNNGG 3 cut(s) 980, 1198, 1545
BsaWI WCCGGW 2 cut(s) 152, 1123
BsaXI ACNNNNNCTCC 2 cut(s) 1070, 1100
Bsc4I CCNNNNNNNGG 5 cut(s) 697, 1199, 1410, 1533, 1551
Bse1I ACTGG 1 cut(s) 791
Bse3DI GCAATG 1 cut(s) 615
BseAI TCCGGA 1 cut(s) 1123
BseBI CCWGG 2 cut(s) 981, 1577
BseDI CCNNGG 3 cut(s) 980, 1198, 1545
BseGI GGATG 4 cut(s) 687, 706, 1420, 1524
BseLI CCNNNNNNNGG 5 cut(s) 697, 1199, 1410, 1533, 1551
BseMI GCAATG 1 cut(s) 615
BseMII CTCAG 7 cut(s) 946, 959, 1116, 1382, 1412, 1431, 1527
BseNI ACTGG 1 cut(s) 791
BseRI GAGGAG 2 cut(s) 668, 1091
BseSI GKGCMC 2 cut(s) 160, 343
BseXI GCAGC 6 cut(s) 1130, 1288, 1336, 1412, 1574, 1628
BseYI CCCAGC 1 cut(s) 1409
BsgI GTGCAG 1 cut(s) 1335
BshFI GGCC 7 cut(s) 319, 372, 426, 561, 705, 1544, 1580
BshNI GGYRCC 1 cut(s) 155
BsiHKAI GWGCWC 1 cut(s) 343
BsiSI CCGG 3 cut(s) 153, 1124, 1193
BslFI GGGAC 1 cut(s) 493
BslI CCNNNNNNNGG 5 cut(s) 697, 1199, 1410, 1533, 1551
BsmAI GTCTC 2 cut(s) 1006, 1216
BsmFI GGGAC 1 cut(s) 493
BsnI GGCC 7 cut(s) 319, 372, 426, 561, 705, 1544, 1580
Bso31I GGTCTC 1 cut(s) 1216
Bsp119I TTCGAA 1 cut(s) 143
Bsp1286I GDGCHC 2 cut(s) 160, 343
Bsp13I TCCGGA 1 cut(s) 1123
Bsp1407I TGTACA 2 cut(s) 202, 1161
Bsp143I GATC 5 cut(s) 355, 990, 1168, 1270, 1417
BspACI CCGC 5 cut(s) 411, 1116, 1279, 1358, 1644
BspANI GGCC 7 cut(s) 319, 372, 426, 561, 705, 1544, 1580
BspCNI CTCAG 7 cut(s) 945, 958, 1115, 1381, 1413, 1432, 1526
BspEI TCCGGA 1 cut(s) 1123
BspHI TCATGA 1 cut(s) 1386
BspLI GGNNCC 2 cut(s) 157, 671
BspMAI CTGCAG 1 cut(s) 1246
BspMI ACCTGC 2 cut(s) 1580, 1610
BspOI GCTAGC 1 cut(s) 684
BspPI GGATC 1 cut(s) 350
BspQI GCTCTTC 1 cut(s) 70
BspT104I TTCGAA 1 cut(s) 143
BspT107I GGYRCC 1 cut(s) 155
BspTNI GGTCTC 1 cut(s) 1216
BsrDI GCAATG 1 cut(s) 615
BsrGI TGTACA 2 cut(s) 202, 1161
BsrI ACTGG 1 cut(s) 791
BssECI CCNNGG 3 cut(s) 980, 1198, 1545
BssMI GATC 5 cut(s) 355, 990, 1168, 1270, 1417
BssNI GRCGYC 1 cut(s) 1136
BssSI CACGAG 1 cut(s) 1570
BssT1I CCWWGG 1 cut(s) 1198
Bst2BI CACGAG 1 cut(s) 1570
Bst2UI CCWGG 2 cut(s) 981, 1577
Bst4CI ACNGT 7 cut(s) 116, 325, 495, 553, 719, 964, 1086
Bst6I CTCTTC 4 cut(s) 70, 1068, 1369, 1482
BstACI GRCGYC 1 cut(s) 1136
BstAPI GCANNNNNTGC 1 cut(s) 1625
BstAUI TGTACA 2 cut(s) 202, 1161
BstBI TTCGAA 1 cut(s) 143
BstC8I GCNNGC 6 cut(s) 419, 450, 682, 753, 1500, 1567
BstDEI CTNAG 8 cut(s) 593, 932, 945, 1102, 1368, 1421, 1440, 1513
BstENI CCTNNNNNAGG 1 cut(s) 1549
BstF5I GGATG 4 cut(s) 687, 706, 1420, 1524
BstKTI GATC 5 cut(s) 358, 993, 1171, 1273, 1420
BstMAI GTCTC 2 cut(s) 1006, 1216
BstMBI GATC 5 cut(s) 355, 990, 1168, 1270, 1417
BstNI CCWGG 2 cut(s) 981, 1577
BstNSI RCATGY 3 cut(s) 421, 778, 1019
BstSCI CCNGG 3 cut(s) 979, 1191, 1575
BstSFI CTRYAG 2 cut(s) 498, 1242
BstSLI GKGCMC 2 cut(s) 160, 343
BstV1I GCAGC 6 cut(s) 1130, 1288, 1336, 1412, 1574, 1628
BstV2I GAAGAC 2 cut(s) 128, 920
BstX2I RGATCY 2 cut(s) 355, 1168
BstXI CCANNNNNNTGG 1 cut(s) 803
BstYI RGATCY 2 cut(s) 355, 1168
BsuI GTATCC 1 cut(s) 1120
BsuRI GGCC 7 cut(s) 319, 372, 426, 561, 705, 1544, 1580
BtrI CACGTC 1 cut(s) 200
BtsCI GGATG 4 cut(s) 687, 706, 1420, 1524
BtsI GCAGTG 1 cut(s) 1260
BtsIMutI CAGTG 3 cut(s) 121, 940, 1260
BveI ACCTGC 2 cut(s) 1580, 1610
Cac8I GCNNGC 6 cut(s) 419, 450, 682, 753, 1500, 1567
CaiI CAGNNNCTG 1 cut(s) 1090
CciI TCATGA 1 cut(s) 1386
Cfr13I GGNCC 3 cut(s) 559, 1195, 1542
CseI GACGC 1 cut(s) 911
Csp6I GTAC 6 cut(s) 203, 303, 375, 874, 1096, 1162
CviAII CATG 9 cut(s) 418, 422, 475, 775, 782, 1016, 1387, 1456, 1529
CviQI GTAC 6 cut(s) 203, 303, 375, 874, 1096, 1162
DdeI CTNAG 8 cut(s) 593, 932, 945, 1102, 1368, 1421, 1440, 1513
DpnI GATC 5 cut(s) 357, 992, 1170, 1272, 1419
DpnII GATC 5 cut(s) 355, 990, 1168, 1270, 1417
DrdI GACNNNNNNGTC 1 cut(s) 1020
DriI GACNNNNNGTC 2 cut(s) 124, 493
DseDI GACNNNNNNGTC 1 cut(s) 1020
EaeI YGGCCR 3 cut(s) 424, 703, 1578
Eam1104I CTCTTC 4 cut(s) 70, 1068, 1369, 1482
Eam1105I GACNNNNNGTC 2 cut(s) 124, 493
EarI CTCTTC 4 cut(s) 70, 1068, 1369, 1482
Eco130I CCWWGG 1 cut(s) 1198
Eco147I AGGCCT 1 cut(s) 319
Eco31I GGTCTC 1 cut(s) 1216
Eco32I GATATC 1 cut(s) 40
Eco47I GGWCC 1 cut(s) 1195
EcoNI CCTNNNNNAGG 1 cut(s) 1549
EcoO109I RGGNCCY 1 cut(s) 1542
EcoRII CCWGG 2 cut(s) 979, 1575
EcoRV GATATC 1 cut(s) 40
EcoT14I CCWWGG 1 cut(s) 1198
EcoT22I ATGCAT 2 cut(s) 423, 840
ErhI CCWWGG 1 cut(s) 1198
FaeI CATG 9 cut(s) 421, 425, 478, 778, 785, 1019, 1390, 1459, 1532
FalI AAGNNNNNCTT 4 cut(s) 74, 106, 231, 263
FaqI GGGAC 1 cut(s) 493
FatI CATG 9 cut(s) 417, 421, 474, 774, 781, 1015, 1386, 1455, 1528
FauI CCCGC 1 cut(s) 1651
FauNDI CATATG 1 cut(s) 1428
FblI GTMKAC 1 cut(s) 1146
Fnu4HI GCNGC 6 cut(s) 1119, 1277, 1350, 1401, 1563, 1617
FokI GGATG 4 cut(s) 674, 713, 1427, 1531
Fsp4HI GCNGC 6 cut(s) 1119, 1277, 1350, 1401, 1563, 1617
FspBI CTAG 4 cut(s) 300, 681, 768, 1551
GluI GCNGC 6 cut(s) 1119, 1277, 1350, 1401, 1563, 1617
GsaI CCCAGC 1 cut(s) 1413
GsuI CTGGAG 1 cut(s) 1364
HaeIII GGCC 7 cut(s) 319, 372, 426, 561, 705, 1544, 1580
HapII CCGG 3 cut(s) 153, 1124, 1193
HgaI GACGC 1 cut(s) 911
Hin1I GRCGYC 1 cut(s) 1136
Hin1II CATG 9 cut(s) 421, 425, 478, 778, 785, 1019, 1390, 1459, 1532
HindIII AAGCTT 1 cut(s) 431
HinfI GANTC 6 cut(s) 528, 587, 627, 881, 1366, 1423
HpaII CCGG 3 cut(s) 153, 1124, 1193
HphI GGTGA 5 cut(s) 377, 969, 976, 997, 1214
Hpy166II GTNNAC 7 cut(s) 203, 341, 377, 796, 1096, 1147, 1162
Hpy188I TCNGA 6 cut(s) 487, 700, 1026, 1033, 1180, 1422
Hpy188III TCNNGA 9 cut(s) 257, 353, 359, 401, 631, 947, 1124, 1343, 1387
Hpy8I GTNNAC 7 cut(s) 203, 341, 377, 796, 1096, 1147, 1162
Hpy99I CGWCG 1 cut(s) 201
HpyAV CCTTC 6 cut(s) 228, 359, 362, 542, 617, 654
HpyCH4III ACNGT 7 cut(s) 116, 325, 495, 553, 719, 964, 1086
HpyCH4IV ACGT 7 cut(s) 133, 147, 199, 405, 866, 1136, 1149
HpyF3I CTNAG 8 cut(s) 593, 932, 945, 1102, 1368, 1421, 1440, 1513
HpySE526I ACGT 7 cut(s) 133, 147, 199, 405, 866, 1136, 1149
Hsp92I GRCGYC 1 cut(s) 1136
Hsp92II CATG 9 cut(s) 421, 425, 478, 778, 785, 1019, 1390, 1459, 1532
Kpn2I TCCGGA 1 cut(s) 1123
Kzo9I GATC 5 cut(s) 355, 990, 1168, 1270, 1417
LguI GCTCTTC 1 cut(s) 70
LmnI GCTCC 2 cut(s) 655, 1126
Lsp1109I GCAGC 6 cut(s) 1130, 1288, 1336, 1412, 1574, 1628
LweI GCATC 3 cut(s) 693, 983, 1291
MaeI CTAG 4 cut(s) 300, 681, 768, 1551
MaeII ACGT 7 cut(s) 133, 147, 199, 405, 866, 1136, 1149
MaeIII GTNAC 7 cut(s) 110, 116, 178, 391, 719, 770, 1556
MalI GATC 5 cut(s) 357, 992, 1170, 1272, 1419
MboI GATC 5 cut(s) 355, 990, 1168, 1270, 1417
MboII GAAGA 8 cut(s) 57, 128, 754, 920, 1000, 1085, 1386, 1499
MflI RGATCY 2 cut(s) 355, 1168
MhlI GDGCHC 2 cut(s) 160, 343
MlsI TGGCCA 2 cut(s) 426, 705
MluNI TGGCCA 2 cut(s) 426, 705
MlyI GAGTC 4 cut(s) 522, 621, 1360, 1432
MmeI TCCRAC 1 cut(s) 1036
Mox20I TGGCCA 2 cut(s) 426, 705
Mph1103I ATGCAT 2 cut(s) 423, 840
MroI TCCGGA 1 cut(s) 1123
MroXI GAANNNNTTC 1 cut(s) 868
MscI TGGCCA 2 cut(s) 426, 705
MseI TTAA 4 cut(s) 14, 86, 555, 1446
Msp20I TGGCCA 2 cut(s) 426, 705
MspA1I CMGCKG 1 cut(s) 1279
MspI CCGG 3 cut(s) 153, 1124, 1193
MspR9I CCNGG 3 cut(s) 981, 1193, 1577
MvaI CCWGG 2 cut(s) 981, 1577
NciI CCSGG 1 cut(s) 1193
NdeI CATATG 1 cut(s) 1428
NdeII GATC 5 cut(s) 355, 990, 1168, 1270, 1417
NheI GCTAGC 1 cut(s) 680
NlaIII CATG 9 cut(s) 421, 425, 478, 778, 785, 1019, 1390, 1459, 1532
NlaIV GGNNCC 2 cut(s) 157, 671
NmeAIII GCCGAG 1 cut(s) 1526
NmuCI GTSAC 4 cut(s) 116, 178, 719, 770
NsiI ATGCAT 2 cut(s) 423, 840
NspI RCATGY 3 cut(s) 421, 778, 1019
NspV TTCGAA 1 cut(s) 143
PaeI GCATGC 1 cut(s) 421
PagI TCATGA 1 cut(s) 1386
PceI AGGCCT 1 cut(s) 319
PciI ACATGT 1 cut(s) 1015
PciSI GCTCTTC 1 cut(s) 70
PcsI WCGNNNNNNNCGW 1 cut(s) 1155
PdmI GAANNNNTTC 1 cut(s) 868
PfeI GAWTC 2 cut(s) 587, 881
PkrI GCNGC 6 cut(s) 1120, 1278, 1351, 1402, 1564, 1618
PleI GAGTC 4 cut(s) 522, 621, 1360, 1431
PpsI GAGTC 4 cut(s) 522, 621, 1360, 1431
PscI ACATGT 1 cut(s) 1015
Psp1406I AACGTT 2 cut(s) 147, 866
Psp6I CCWGG 2 cut(s) 979, 1575
PspFI CCCAGC 1 cut(s) 1409
PspGI CCWGG 2 cut(s) 979, 1575
PspN4I GGNNCC 2 cut(s) 157, 671
PspPI GGNCC 3 cut(s) 559, 1195, 1542
PstI CTGCAG 1 cut(s) 1246
PstNI CAGNNNCTG 1 cut(s) 1090
PsuI RGATCY 2 cut(s) 355, 1168
RsaI GTAC 6 cut(s) 204, 304, 376, 875, 1097, 1163
RsaNI GTAC 6 cut(s) 203, 303, 375, 874, 1096, 1162
SapI GCTCTTC 1 cut(s) 70
SaqAI TTAA 4 cut(s) 14, 86, 555, 1446
SatI GCNGC 6 cut(s) 1119, 1277, 1350, 1401, 1563, 1617
Sau3AI GATC 5 cut(s) 355, 990, 1168, 1270, 1417
Sau96I GGNCC 3 cut(s) 559, 1195, 1542
SchI GAGTC 4 cut(s) 522, 621, 1360, 1432
ScrFI CCNGG 3 cut(s) 981, 1193, 1577
SduI GDGCHC 2 cut(s) 160, 343
SfaNI GCATC 3 cut(s) 693, 983, 1291
SfcI CTRYAG 2 cut(s) 498, 1242
SfuI TTCGAA 1 cut(s) 143
SinI GGWCC 1 cut(s) 1195
SmlI CTYRAG 2 cut(s) 629, 650
SmoI CTYRAG 2 cut(s) 629, 650
SphI GCATGC 1 cut(s) 421
SseBI AGGCCT 1 cut(s) 319
SsiI CCGC 5 cut(s) 411, 1116, 1279, 1358, 1644
SspMI CTAG 4 cut(s) 300, 681, 768, 1551
StuI AGGCCT 1 cut(s) 319
StyD4I CCNGG 3 cut(s) 979, 1191, 1575
StyI CCWWGG 1 cut(s) 1198
TaaI ACNGT 7 cut(s) 116, 325, 495, 553, 719, 964, 1086
TaiI ACGT 7 cut(s) 136, 150, 202, 408, 869, 1139, 1152
TaqI TCGA 4 cut(s) 23, 128, 143, 585
TatI WGTACW 2 cut(s) 202, 1161
TfiI GAWTC 2 cut(s) 587, 881
Tru1I TTAA 4 cut(s) 14, 86, 555, 1446
Tru9I TTAA 4 cut(s) 14, 86, 555, 1446
TscAI CASTG 3 cut(s) 121, 940, 1260
TseFI GTSAC 4 cut(s) 116, 178, 719, 770
TseI GCWGC 6 cut(s) 1118, 1276, 1349, 1400, 1562, 1616
Tsp45I GTSAC 4 cut(s) 116, 178, 719, 770
TspDTI ATGAA 4 cut(s) 258, 798, 1490, 1500
TspGWI ACGGA 1 cut(s) 530
TspRI CASTG 3 cut(s) 121, 940, 1260
VneI GTGCAC 1 cut(s) 339
VpaK11BI GGWCC 1 cut(s) 1195
XagI CCTNNNNNAGG 1 cut(s) 1549
XapI RAATTY 6 cut(s) 8, 26, 229, 333, 458, 1477
XceI RCATGY 3 cut(s) 421, 778, 1019
XcmI CCANNNNNNNNNTGG 1 cut(s) 1138
XmiI GTMKAC 1 cut(s) 1146
XmnI GAANNNNTTC 1 cut(s) 868
XspI CTAG 4 cut(s) 300, 681, 768, 1551
ZraI GACGTC 1 cut(s) 1137
Zsp2I ATGCAT 2 cut(s) 423, 840
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.