pycom10g20000

VWA / Hh protein intein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0000360
Physical Location & Seq
Reverse (-)
23020655 .. 23024373
3719 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g20000.2

Sequence Viewer

Length: 2049 bp
ATGGCCGCTGCTACGTGGCGGAAGCTGAAGAAGTCACTGACGCACAAGCTCTCATTCCGCTCCTCCGATTCGTCGTCCACCTCGTCGGCGTCGGCGTCTCGGCCGCCTCGTCCTCCTCCTCCCGCCTTAACCTCCTCCGCCACCCTCCGCCCCTCTCCGTCGTCTCGCACCTCCTCCTCCTCCATCTCCTCCAAGCTTTCCCGCAGCTTCAGCTCTCGCATCTCCAAGAAAAATTGCGCCATTTGCCTGAGTAACCTGAGAGCAGGGCAAGGCCAAGCCATCTTCACTGCTGAGTGCTCCCACTCTTTCCACTTCGTTTGCATTGCCAACAATGTCAAACATGGAAATATTTGCTGTCCCATCTGCCGTTCCAAGTGGAATGATGTGCCTTTCCAAGCTCCCATCAACGTTGCAAATACTCAACAAAACCATTTGGGTCACATCCATAATGGTTTTAACCATCGTTACCAGTCTCACCTCCCATTGCTGCCCCAGGTCCATCCAGAGCCACTAAGCTTCTCAGATGATGAGCCTCTCCCGTCCACCTCACCTAGTCGATTCTCTGGCCCCCAAAATGTTGCAATCAGGGCTTATACAGAGTACTCTGCTATCCCTTTTGCAGAATCTCGCCCAACATTTCCTGTTCTTGTCAGCGTTCGAGCACCACCCCTTCAAGGAGGTGCGAACAGCCATGGCCGTACCCCCATTGACCTTGTAACAGTTCTAGATGTAAGTGGCAGCATGTTTGGCACAAAAATTGCCCTTCTCAAGCGCGCTGTCAGATTTGTCATACAAAACTTGGGGCCTTATGACCGGCTTGCCATAGTTTCATTCTCAACTACTGCTAAAAGGGTCTTTCCTCTCCGAAGAATGACTGTTGATGGTCATGAAAGCGCCTTTCTAGCAGTCGAGTCTCTTAGAGCAAATGGTGGAACCGACATTGTTGGAGGACTCAAGAAAGGAGTTCAGGTCCTTGAAGAACGCCGGGCAAGGAATCCAGTTTCTAGCATCATCCTCTTATCTGATGGAAGAGACAATTGCAACTATGACTCACAGAATACTTCAAGTCCAAACCGGGTCTCTGAGTTCTTGAACCAATTGCCAGCTTCGGTACATTGTAGCAATGTTCCCATCCACACATTTGGGTTTGGCACTGACCATGATGCCACTACTATGCATGCTATATCTGATTCATCCAGTGGCACTTTTTCCTTCATTGAGTCAGTTGCTATGATACAAGATGCCTTTGCCATGTGCATTGGTGGTCTTCTCAGTGTTGTGGCTCAGGAACTTCGACTCACAGTTAGGTCAGCATCGCACGGGGTGCAGATTGTATCAATACCATCAGGGAGTCATGTGAGTGAGATTTCTGACGAGTGTCAACAAGGTGCTGTTGATGTTGGAAATATGTATGCAGAAGAGGAGAAACAGTTTCTGGTGTACCTTTTAGTTCCAACATTGTCAGCAACTGGAAACAGGGAAGGCATAAGGAAGACATCACTGTTAGATGTGTCATGCGTGTACAAGGATCTAGGCTCAAATGAGTCAACCCATGTGCAAGGTGAGAGAGTAGAGATATTAAGACCCGAGATTTGGTCCCCTGCAGATGAGGCAGTGTGTTTGGAGGTCGACCGTCAGAGGAATAGAATTCTGGTAGCTGAAGGTATTGCAGAGGCACAAGTAATGGCTGAAATGGGAAATCTAGAAGGTGCACAGGCTATTTTGGCACAACGAATAGCAACCATCTTAACATCACCTGCAGCACAAGCTGGGGATGGTCTCTGTAATTGTCTGGAAGCTGAGCTTCGAGAAATCAGGGATAGAATGGCTAGTATGGAATCATACACGCAGACCGGGCGTGCTTATGTTCTTTCAGGGTTAAGCTCACATTCATGGCAGAGAGCCACAACTAGGGGTGACATGACAACCCAGATTATGACCATGAATGAAGGTGGAAACTCACGCAGCAGCGCCGTTGGCTATGAGACACCTACTATGGTTAACATGGTAAGGATGTCGCAGAATCTTGGGGCTAATCGCACGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VWA VWA_3 Vwaint zf-RING_11 zf-RING_2

Protein Analysis

683

Amino Acids

73.78

Weight (kDa)

8.45

Isoelectric Point (pI)

51.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000360)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11691 FvH4_3g11693 FvH4_3g11740 FvH4_3g11750 FvH4_3g11770 FvH4_3g11771 FvH4_3g11772 FvH4_3g11780
malus_domestica MD10G1239000.v1.1
prunus_persica Prupe.4G105400_v2.0.a1
pyrus_communis pycom10g20000
rosa_chinensis RchiOBHm_Chr5g0018981 RchiOBHm_Chr5g0018991 RchiOBHm_Chr5g0019101 RchiOBHm_Chr5g0019111 RchiOBHm_Chr5g0019121 RchiOBHm_Chr5g0019151 RchiOBHm_Chr5g0019161 RchiOBHm_Chr5g0019171 RchiOBHm_Chr5g0019181 RchiOBHm_Chr5g0019201
rosa_laevigata RLG00000032411 RLG00000032418 RLG00000032419 RLG00000032420 RLG00000032421 RLG00000032423 RLG00000032424 RLG00000032426
rosa_multiflora Rmu_co8475613.1_g000001 Rmu_co8503587.1_g000001 Rmu_sc0000563.1_g000023 Rmu_sc0000563.1_g000027 Rmu_sc0002142.1_g000001 Rmu_sc0002142.1_g000003 Rmu_sc0002142.1_g000004 Rmu_sc0002142.1_g000011 Rmu_sc0002142.1_g000022 Rmu_sc0002561.1_g000002 Rmu_sc0042836.1_g000001
rosa_roxburghii Rroxscaffold_1G00058790 Rroxscaffold_1G00058810 Rroxscaffold_1G00058820 Rroxscaffold_1G00058840 Rroxscaffold_1G00058850 Rroxscaffold_1G00058860 Rroxscaffold_1G00058880 Rroxscaffold_1G00058910 Rroxscaffold_1G00074090 Rroxscaffold_1G00074110 Rroxscaffold_1G00074120 Rroxscaffold_1G00074130 Rroxscaffold_1G00074150
rosa_rugosa Rorug05G0046800 Rorug05G0047200 Rorug05G0047300 Rorug05G0047400 Rorug05G0047600 Rorug05G0047600 Rorug05G0047800 Rorug05G0047900 Rorug05G0048000 Rorug05G0048100 Rorug05G0048100 Rorug05G0048200 Rorug05G0048300
rosa_samantha Rh4DG102800 Rh5AG138700 Rh5AG138900 Rh5AG139000 Rh5AG139100 Rh5AG139200 Rh5AG139400 Rh5AG139500 Rh5AG139600 Rh5BG137400 Rh5BG137600 Rh5BG137800 Rh5BG138100 Rh5BG138200 Rh5BG138300 Rh5BG138500 Rh5CG148800 Rh5CG148900 Rh5CG149200 Rh5CG149300 Rh5CG149400 Rh5CG149600 Rh5CG149700 Rh5CG150000 Rh5DG138200 Rh5DG138300 Rh5DG138500 Rh5DG138600 Rh5DG138700 Rh5DG138900 Rh5DG139000 Rh5DG139200
rosa_wichuraiana Rw5G012290 Rw5G012330 Rw5G012340 Rw5G012350 Rw5G012370 Rw5G012380 Rw5G012390 Rw5G012410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1765
Acc36I ACCTGC 1 cut(s) 1765
AccBSI CCGCTC 1 cut(s) 60
AccI GTMKAC 1 cut(s) 1629
AccII CGCG 1 cut(s) 774
AciI CCGC 8 cut(s) 6, 19, 58, 104, 123, 138, 148, 202
AclI AACGTT 1 cut(s) 408
AclWI GGATC 1 cut(s) 1536
AcoI YGGCCR 3 cut(s) 3, 101, 694
AcsI RAATTY 1 cut(s) 1647
AcuI CTGAAG 3 cut(s) 47, 193, 1680
AcyI GRCGYC 2 cut(s) 89, 95
AdeI CACNNNGTG 1 cut(s) 1324
AfaI GTAC 5 cut(s) 602, 700, 1113, 1442, 1523
AfiI CCNNNNNNNGG 3 cut(s) 674, 1593, 1911
AgsI TTSAA 4 cut(s) 674, 977, 1065, 1093
AjnI CCWGG 1 cut(s) 492
Alw21I GWGCWC 3 cut(s) 299, 664, 1714
Alw26I GTCTC 8 cut(s) 102, 168, 477, 918, 1026, 1084, 1784, 1979
Alw44I GTGCAC 1 cut(s) 1710
AlwI GGATC 1 cut(s) 1536
AlwNI CAGNNNCTG 2 cut(s) 1435, 1469
Ama87I CYCGRG 1 cut(s) 1586
AoxI GGCC 6 cut(s) 3, 101, 271, 565, 694, 803
ApaLI GTGCAC 1 cut(s) 1710
ApeKI GCWGC 7 cut(s) 8, 204, 487, 738, 1760, 1965, 1968
ApoI RAATTY 1 cut(s) 1647
ArsI GACNNNNNNTTYG 2 cut(s) 1575, 1607
AspLEI GCGC 5 cut(s) 239, 774, 776, 896, 1973
AspS9I GGNCC 5 cut(s) 496, 566, 803, 970, 1596
AsuC2I CCSGG 3 cut(s) 986, 1076, 1855
AsuHPI GGTGA 5 cut(s) 467, 540, 1574, 1746, 1928
AvaI CYCGRG 1 cut(s) 1586
AvaII GGWCC 3 cut(s) 496, 970, 1596
BaeGI GKGCMC 1 cut(s) 1714
BbsI GAAGAC 2 cut(s) 1259, 1499
Bbv12I GWGCWC 3 cut(s) 299, 664, 1714
BbvI GCAGC 6 cut(s) 216, 474, 750, 1772, 1977, 1980
BceAI ACGGC 3 cut(s) 351, 681, 1958
BciT130I CCWGG 1 cut(s) 494
BcnI CCSGG 3 cut(s) 986, 1076, 1855
BcoDI GTCTC 8 cut(s) 102, 168, 477, 918, 1026, 1084, 1784, 1979
BfaI CTAG 8 cut(s) 552, 725, 902, 1005, 1532, 1703, 1830, 1911
BfmI CTRYAG 2 cut(s) 1602, 1758
BfoI RGCGCY 2 cut(s) 897, 1974
BfuAI ACCTGC 1 cut(s) 1765
BisI GCNGC 9 cut(s) 6, 9, 104, 205, 488, 739, 1761, 1966, 1969
BlpI GCTNAGC 1 cut(s) 1800
BlsI GCNGC 9 cut(s) 7, 10, 105, 206, 489, 740, 1762, 1967, 1970
BmcAI AGTACT 1 cut(s) 602
Bme1390I CCNGG 4 cut(s) 494, 986, 1076, 1855
Bme18I GGWCC 3 cut(s) 496, 970, 1596
BmeT110I CYCGRG 1 cut(s) 1586
BmgT120I GGNCC 5 cut(s) 496, 566, 803, 970, 1596
BmiI GGNNCC 4 cut(s) 568, 804, 934, 1598
BmrFI CCNGG 4 cut(s) 494, 986, 1076, 1855
BmsI GCATC 5 cut(s) 228, 1017, 1153, 1231, 1322
BoxI GACNNNNGTC 1 cut(s) 1377
BpiI GAAGAC 2 cut(s) 1259, 1499
Bpu10I CCTNAGC 1 cut(s) 1284
Bpu1102I GCTNAGC 1 cut(s) 1800
BpuEI CTTGAG 2 cut(s) 752, 938
BpuMI CCSGG 3 cut(s) 986, 1076, 1855
BsaAI YACGTR 1 cut(s) 15
BsaHI GRCGYC 2 cut(s) 89, 95
BsaI GGTCTC 2 cut(s) 1084, 1784
BsaJI CCNNGG 2 cut(s) 492, 691
BsaXI ACNNNNNCTCC 4 cut(s) 954, 984, 1415, 1445
Bsc4I CCNNNNNNNGG 3 cut(s) 674, 1593, 1911
Bse118I RCCGGY 1 cut(s) 813
Bse1I ACTGG 4 cut(s) 469, 998, 1197, 1474
Bse3DI GCAATG 3 cut(s) 321, 482, 1129
BseBI CCWGG 1 cut(s) 494
BseDI CCNNGG 2 cut(s) 492, 691
BseGI GGATG 7 cut(s) 441, 499, 1011, 1131, 1193, 1780, 2019
BseLI CCNNNNNNNGG 3 cut(s) 674, 1593, 1911
BseMI GCAATG 3 cut(s) 321, 482, 1129
BseMII CTCAG 8 cut(s) 239, 248, 282, 534, 1074, 1285, 1298, 1791
BseNI ACTGG 4 cut(s) 469, 998, 1197, 1474
BsePI GCGCGC 1 cut(s) 772
BseRI GAGGAG 9 cut(s) 52, 105, 108, 124, 163, 166, 169, 178, 1436
BseSI GKGCMC 1 cut(s) 1714
BseX3I CGGCCG 1 cut(s) 101
BseXI GCAGC 6 cut(s) 216, 474, 750, 1772, 1977, 1980
BseYI CCCAGC 1 cut(s) 1769
BsgI GTGCAG 1 cut(s) 1346
Bsh1236I CGCG 1 cut(s) 774
Bsh1285I CGRYCG 2 cut(s) 104, 1633
BshFI GGCC 6 cut(s) 5, 103, 273, 567, 696, 805
BsiEI CGRYCG 2 cut(s) 104, 1633
BsiHKAI GWGCWC 3 cut(s) 299, 664, 1714
BsiHKCI CYCGRG 1 cut(s) 1586
BsiSI CCGG 4 cut(s) 814, 985, 1075, 1854
BslFI GGGAC 2 cut(s) 342, 1582
BslI CCNNNNNNNGG 3 cut(s) 674, 1593, 1911
BsmAI GTCTC 8 cut(s) 102, 168, 477, 918, 1026, 1084, 1784, 1979
BsmBI CGTCTC 2 cut(s) 102, 168
BsmFI GGGAC 2 cut(s) 342, 1582
BsnI GGCC 6 cut(s) 5, 103, 273, 567, 696, 805
Bso31I GGTCTC 2 cut(s) 1084, 1784
BsoBI CYCGRG 1 cut(s) 1586
Bsp1286I GDGCHC 3 cut(s) 299, 664, 1714
Bsp1407I TGTACA 1 cut(s) 1521
Bsp143I GATC 1 cut(s) 1528
Bsp1720I GCTNAGC 1 cut(s) 1800
Bsp19I CCATGG 1 cut(s) 691
BspACI CCGC 8 cut(s) 6, 19, 58, 104, 123, 138, 148, 202
BspANI GGCC 6 cut(s) 5, 103, 273, 567, 696, 805
BspCNI CTCAG 8 cut(s) 240, 249, 283, 533, 1075, 1284, 1297, 1792
BspFNI CGCG 1 cut(s) 774
BspHI TCATGA 1 cut(s) 886
BspLI GGNNCC 4 cut(s) 568, 804, 934, 1598
BspMAI CTGCAG 2 cut(s) 1606, 1762
BspMI ACCTGC 1 cut(s) 1765
BspPI GGATC 1 cut(s) 1536
BspTNI GGTCTC 2 cut(s) 1084, 1784
BsrBI CCGCTC 1 cut(s) 60
BsrDI GCAATG 3 cut(s) 321, 482, 1129
BsrFI RCCGGY 1 cut(s) 813
BsrGI TGTACA 1 cut(s) 1521
BsrI ACTGG 4 cut(s) 469, 998, 1197, 1474
BssAI RCCGGY 1 cut(s) 813
BssECI CCNNGG 2 cut(s) 492, 691
BssHII GCGCGC 1 cut(s) 772
BssMI GATC 1 cut(s) 1528
BssNI GRCGYC 2 cut(s) 89, 95
BssT1I CCWWGG 1 cut(s) 691
Bst2UI CCWGG 1 cut(s) 494
Bst4CI ACNGT 6 cut(s) 721, 877, 1303, 1431, 1503, 1634
Bst6I CTCTTC 2 cut(s) 1024, 1413
BstACI GRCGYC 2 cut(s) 89, 95
BstAPI GCANNNNNTGC 1 cut(s) 1324
BstAUI TGTACA 1 cut(s) 1521
BstBAI YACGTR 1 cut(s) 15
BstC8I GCNNGC 5 cut(s) 774, 819, 1104, 1179, 1860
BstDSI CCRYGG 1 cut(s) 691
BstF5I GGATG 7 cut(s) 441, 499, 1011, 1131, 1193, 1780, 2019
BstFNI CGCG 1 cut(s) 774
BstH2I RGCGCY 2 cut(s) 897, 1974
BstHHI GCGC 5 cut(s) 239, 774, 776, 896, 1973
BstKTI GATC 1 cut(s) 1531
BstMAI GTCTC 8 cut(s) 102, 168, 477, 918, 1026, 1084, 1784, 1979
BstMBI GATC 1 cut(s) 1528
BstMCI CGRYCG 2 cut(s) 104, 1633
BstNI CCWGG 1 cut(s) 494
BstNSI RCATGY 2 cut(s) 745, 1181
BstPAI GACNNNNGTC 1 cut(s) 1377
BstSCI CCNGG 4 cut(s) 492, 984, 1074, 1853
BstSFI CTRYAG 2 cut(s) 1602, 1758
BstSLI GKGCMC 1 cut(s) 1714
BstUI CGCG 1 cut(s) 774
BstV1I GCAGC 6 cut(s) 216, 474, 750, 1772, 1977, 1980
BstV2I GAAGAC 2 cut(s) 1259, 1499
BstX2I RGATCY 1 cut(s) 1528
BstXI CCANNNNNNTGG 1 cut(s) 1142
BstYI RGATCY 1 cut(s) 1528
BstZI CGGCCG 1 cut(s) 101
BsuRI GGCC 6 cut(s) 5, 103, 273, 567, 696, 805
BtgI CCRYGG 1 cut(s) 691
BtgZI GCGATG 1 cut(s) 1299
BtsCI GGATG 7 cut(s) 441, 499, 1011, 1131, 1193, 1780, 2019
BtsI GCAGTG 2 cut(s) 285, 1620
BtsIMutI CAGTG 7 cut(s) 35, 285, 1152, 1204, 1279, 1499, 1620
BveI ACCTGC 1 cut(s) 1765
Cac8I GCNNGC 5 cut(s) 774, 819, 1104, 1179, 1860
CaiI CAGNNNCTG 2 cut(s) 1435, 1469
CciI TCATGA 1 cut(s) 886
CfoI GCGC 5 cut(s) 239, 774, 776, 896, 1973
Cfr10I RCCGGY 1 cut(s) 813
Cfr13I GGNCC 5 cut(s) 496, 566, 803, 970, 1596
CseI GACGC 3 cut(s) 49, 78, 84
Csp6I GTAC 5 cut(s) 601, 699, 1112, 1441, 1522
CspCI CAANNNNNGTGG 2 cut(s) 299, 334
CviQI GTAC 5 cut(s) 601, 699, 1112, 1441, 1522
DpnI GATC 1 cut(s) 1530
DpnII GATC 1 cut(s) 1528
DraIII CACNNNGTG 1 cut(s) 1324
EaeI YGGCCR 3 cut(s) 3, 101, 694
EagI CGGCCG 1 cut(s) 101
Eam1104I CTCTTC 2 cut(s) 1024, 1413
EarI CTCTTC 2 cut(s) 1024, 1413
EciI GGCGGA 3 cut(s) 34, 127, 137
EclXI CGGCCG 1 cut(s) 101
Eco130I CCWWGG 1 cut(s) 691
Eco31I GGTCTC 2 cut(s) 1084, 1784
Eco47I GGWCC 3 cut(s) 496, 970, 1596
Eco52I CGGCCG 1 cut(s) 101
Eco57I CTGAAG 3 cut(s) 47, 193, 1680
Eco88I CYCGRG 1 cut(s) 1586
EcoO109I RGGNCCY 2 cut(s) 803, 970
EcoRI GAATTC 1 cut(s) 1647
EcoRII CCWGG 1 cut(s) 492
EcoT14I CCWWGG 1 cut(s) 691
EcoT22I ATGCAT 1 cut(s) 1179
ErhI CCWWGG 1 cut(s) 691
Esp3I CGTCTC 2 cut(s) 102, 168
FalI AAGNNNNNCTT 2 cut(s) 1788, 1820
FaqI GGGAC 2 cut(s) 342, 1582
FauI CCCGC 2 cut(s) 130, 209
FblI GTMKAC 1 cut(s) 1629
Fnu4HI GCNGC 9 cut(s) 6, 9, 104, 205, 488, 739, 1761, 1966, 1969
FokI GGATG 7 cut(s) 428, 486, 998, 1118, 1180, 1787, 2026
Fsp4HI GCNGC 9 cut(s) 6, 9, 104, 205, 488, 739, 1761, 1966, 1969
FspBI CTAG 8 cut(s) 552, 725, 902, 1005, 1532, 1703, 1830, 1911
GlaI GCGC 5 cut(s) 238, 773, 775, 895, 1972
GluI GCNGC 9 cut(s) 6, 9, 104, 205, 488, 739, 1761, 1966, 1969
GsaI CCCAGC 1 cut(s) 1773
HaeII RGCGCY 2 cut(s) 897, 1974
HaeIII GGCC 6 cut(s) 5, 103, 273, 567, 696, 805
HapII CCGG 4 cut(s) 814, 985, 1075, 1854
HgaI GACGC 3 cut(s) 49, 78, 84
HhaI GCGC 5 cut(s) 239, 774, 776, 896, 1973
Hin1I GRCGYC 2 cut(s) 89, 95
Hin6I GCGC 5 cut(s) 237, 772, 774, 894, 1971
HinP1I GCGC 5 cut(s) 237, 772, 774, 894, 1971
HincII GTYRAC 4 cut(s) 1382, 1548, 1630, 2002
HindII GTYRAC 4 cut(s) 1382, 1548, 1630, 2002
HindIII AAGCTT 2 cut(s) 194, 514
HpaI GTTAAC 1 cut(s) 2002
HpaII CCGG 4 cut(s) 814, 985, 1075, 1854
HphI GGTGA 5 cut(s) 467, 540, 1574, 1746, 1928
Hpy166II GTNNAC 9 cut(s) 78, 543, 1382, 1441, 1522, 1548, 1630, 1712, 2002
Hpy188I TCNGA 9 cut(s) 67, 523, 782, 866, 1024, 1084, 1189, 1372, 1638
Hpy188III TCNNGA 9 cut(s) 503, 725, 887, 955, 1090, 1286, 1703, 1793, 1808
Hpy8I GTNNAC 9 cut(s) 78, 543, 1382, 1441, 1522, 1548, 1630, 1712, 2002
Hpy99I CGWCG 4 cut(s) 76, 88, 94, 163
HpyAV CCTTC 7 cut(s) 680, 773, 1222, 1475, 1655, 1700, 1943
HpyCH4III ACNGT 6 cut(s) 721, 877, 1303, 1431, 1503, 1634
HpyCH4IV ACGT 2 cut(s) 14, 408
HpySE526I ACGT 2 cut(s) 14, 408
Hsp92I GRCGYC 2 cut(s) 89, 95
HspAI GCGC 5 cut(s) 237, 772, 774, 894, 1971
KspAI GTTAAC 1 cut(s) 2002
Kzo9I GATC 1 cut(s) 1528
LmnI GCTCC 3 cut(s) 65, 302, 403
Lsp1109I GCAGC 6 cut(s) 216, 474, 750, 1772, 1977, 1980
LweI GCATC 5 cut(s) 228, 1017, 1153, 1231, 1322
MaeI CTAG 8 cut(s) 552, 725, 902, 1005, 1532, 1703, 1830, 1911
MaeII ACGT 2 cut(s) 14, 408
MaeIII GTNAC 6 cut(s) 33, 251, 437, 464, 715, 1916
MalI GATC 1 cut(s) 1530
MbiI CCGCTC 1 cut(s) 60
MboI GATC 1 cut(s) 1528
MboII GAAGA 8 cut(s) 40, 274, 879, 989, 1041, 1259, 1430, 1504
MfeI CAATTG 2 cut(s) 1036, 1097
MflI RGATCY 1 cut(s) 1528
MhlI GDGCHC 3 cut(s) 299, 664, 1714
MluCI AATT 6 cut(s) 232, 756, 1036, 1097, 1647, 1786
MlyI GAGTC 7 cut(s) 920, 945, 1043, 1229, 1290, 1360, 1553
MmeI TCCRAC 3 cut(s) 925, 1381, 1478
Mph1103I ATGCAT 1 cut(s) 1179
MseI TTAA 6 cut(s) 128, 456, 1580, 1748, 1880, 2001
MslI CAYNNNNRTG 3 cut(s) 1172, 1359, 1891
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 4 cut(s) 814, 985, 1075, 1854
MspR9I CCNGG 4 cut(s) 494, 986, 1076, 1855
MunI CAATTG 2 cut(s) 1036, 1097
MvaI CCWGG 1 cut(s) 494
MvnI CGCG 1 cut(s) 774
NciI CCSGG 3 cut(s) 986, 1076, 1855
NcoI CCATGG 1 cut(s) 691
NdeII GATC 1 cut(s) 1528
NlaIV GGNNCC 4 cut(s) 568, 804, 934, 1598
NmeAIII GCCGAG 1 cut(s) 79
NmuCI GTSAC 3 cut(s) 33, 437, 1916
NsiI ATGCAT 1 cut(s) 1179
NspI RCATGY 2 cut(s) 745, 1181
PaeI GCATGC 1 cut(s) 1181
PagI TCATGA 1 cut(s) 886
PaqCI CACCTGC 1 cut(s) 1765
PauI GCGCGC 1 cut(s) 772
PcsI WCGNNNNNNNCGW 3 cut(s) 80, 92, 106
PfeI GAWTC 7 cut(s) 68, 558, 623, 994, 1190, 1838, 2023
PkrI GCNGC 9 cut(s) 7, 10, 105, 206, 489, 740, 1762, 1967, 1970
PleI GAGTC 7 cut(s) 919, 945, 1043, 1228, 1290, 1359, 1552
PpsI GAGTC 7 cut(s) 919, 945, 1043, 1228, 1290, 1359, 1552
Ppu21I YACGTR 1 cut(s) 15
PpuMI RGGWCCY 1 cut(s) 970
PshAI GACNNNNGTC 1 cut(s) 1377
Psp1406I AACGTT 1 cut(s) 408
Psp5II RGGWCCY 1 cut(s) 970
Psp6I CCWGG 1 cut(s) 492
PspFI CCCAGC 1 cut(s) 1769
PspGI CCWGG 1 cut(s) 492
PspN4I GGNNCC 4 cut(s) 568, 804, 934, 1598
PspPI GGNCC 5 cut(s) 496, 566, 803, 970, 1596
PspPPI RGGWCCY 1 cut(s) 970
PstI CTGCAG 2 cut(s) 1606, 1762
PstNI CAGNNNCTG 2 cut(s) 1435, 1469
PsuI RGATCY 1 cut(s) 1528
PteI GCGCGC 1 cut(s) 772
RsaI GTAC 5 cut(s) 602, 700, 1113, 1442, 1523
RsaNI GTAC 5 cut(s) 601, 699, 1112, 1441, 1522
RseI CAYNNNNRTG 3 cut(s) 1172, 1359, 1891
SalI GTCGAC 1 cut(s) 1628
SaqAI TTAA 6 cut(s) 128, 456, 1580, 1748, 1880, 2001
SatI GCNGC 9 cut(s) 6, 9, 104, 205, 488, 739, 1761, 1966, 1969
Sau3AI GATC 1 cut(s) 1528
Sau96I GGNCC 5 cut(s) 496, 566, 803, 970, 1596
ScaI AGTACT 1 cut(s) 602
SchI GAGTC 7 cut(s) 920, 945, 1043, 1229, 1290, 1360, 1553
ScrFI CCNGG 4 cut(s) 494, 986, 1076, 1855
SduI GDGCHC 3 cut(s) 299, 664, 1714
SfaNI GCATC 5 cut(s) 228, 1017, 1153, 1231, 1322
SfcI CTRYAG 2 cut(s) 1602, 1758
SinI GGWCC 3 cut(s) 496, 970, 1596
SmiMI CAYNNNNRTG 3 cut(s) 1172, 1359, 1891
SmlI CTYRAG 2 cut(s) 767, 953
SmoI CTYRAG 2 cut(s) 767, 953
SphI GCATGC 1 cut(s) 1181
Sse9I AATT 6 cut(s) 232, 756, 1036, 1097, 1647, 1786
SsiI CCGC 8 cut(s) 6, 19, 58, 104, 123, 138, 148, 202
SspI AATATT 1 cut(s) 349
SspMI CTAG 8 cut(s) 552, 725, 902, 1005, 1532, 1703, 1830, 1911
StyD4I CCNGG 4 cut(s) 492, 984, 1074, 1853
StyI CCWWGG 1 cut(s) 691
TaaI ACNGT 6 cut(s) 721, 877, 1303, 1431, 1503, 1634
TaiI ACGT 2 cut(s) 17, 411
TaqI TCGA 6 cut(s) 556, 658, 909, 1294, 1629, 1807
TasI AATT 6 cut(s) 232, 756, 1036, 1097, 1647, 1786
TatI WGTACW 2 cut(s) 600, 1521
TauI GCSGC 2 cut(s) 8, 106
TfiI GAWTC 7 cut(s) 68, 558, 623, 994, 1190, 1838, 2023
Tru1I TTAA 6 cut(s) 128, 456, 1580, 1748, 1880, 2001
Tru9I TTAA 6 cut(s) 128, 456, 1580, 1748, 1880, 2001
TscAI CASTG 7 cut(s) 42, 292, 1159, 1204, 1279, 1506, 1620
TseFI GTSAC 3 cut(s) 33, 437, 1916
TseI GCWGC 7 cut(s) 8, 204, 487, 738, 1760, 1965, 1968
Tsp45I GTSAC 3 cut(s) 33, 437, 1916
TspDTI ATGAA 7 cut(s) 819, 903, 1182, 1204, 1881, 1958, 1962
TspGWI ACGGA 1 cut(s) 147
TspRI CASTG 7 cut(s) 42, 292, 1159, 1204, 1279, 1506, 1620
VneI GTGCAC 1 cut(s) 1710
VpaK11BI GGWCC 3 cut(s) 496, 970, 1596
XapI RAATTY 1 cut(s) 1647
XbaI TCTAGA 2 cut(s) 724, 1702
XceI RCATGY 2 cut(s) 745, 1181
XmiI GTMKAC 1 cut(s) 1629
XspI CTAG 8 cut(s) 552, 725, 902, 1005, 1532, 1703, 1830, 1911
ZrmI AGTACT 1 cut(s) 602
Zsp2I ATGCAT 1 cut(s) 1179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.