Rmu_co8503587.1_g000001

pre-mRNA 3'-splice site binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8503587.1
OG0000360
Physical Location & Seq
Reverse (-)
512 .. 3120
2609 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8503587.1_g000001.1.cds

Sequence Viewer

Length: 2260 bp
acagggttctgttgctggtgggcttggggatcctgatcgaattttttttggtggattcccttactactttaaagaagaacagatcagggagttggtagagtccttcggaccccttcgcagttttgaactagtgacagatggaggcagaggaaacaactcagttgctgcccattgcgtttaccaggatgtttcagttacagacattgcttgtcatggcctgaatgggattcgaatcggtgcccagacactgattgctaggcgtgctaatcatggtgccaaccagcagccggaaccacattcttcttctcaagaaaattgcagtatttgctggtgtagcctgacaactggccaaggccaagcgatctttactgctgagtgctcccaccgtttccactacccttgtattgccaacaatgtccagcatggaaatctttactgccctgtctgcagagtaaaatgggacaagaataatgtacctttccaagttcctcctcctcaacagaccaggatccctttcaggcagttcccaatgctgcagcaaccccattggaattatcctcacctcccatatcagcagccgcattggaatcatcctcagtacccatttgagcagcaaccccaggaccctcctgagccacttaccttctctgatgatgaacctctccagtcattgacctcacctgttcaatcctcctcccaccagaatgtgacagttacgacttatgcagagtactctgctatctctgctgcggagtctagctcaaagtttcctgtcctggtcagcatccgtgcaccaccccttaaggattctgagggccatggtcgtactcccattgaccttgtaacagttctggatgtaagtggcagcatgactggcgcgaagcttaaccttgtcaagcgtgctgtcaaatttgtcatagaaaacttggggtcttcagacagactttctattgtctcattctcatcaaattctaagagagttcttcctctccaaaggatgactgctgatggccgtgaaagtgccatactagccatcgagtctcttagagcaggtggtggaactaacattgccgaagcactcaagaaaggaattaaggtccttgaagatcgaagggaaaggaacccagttgctagcatcatcctcttatccgatggccaagataactactgtcgaagtggaagccaaattttgaaccaattgcctgcttccattcgttctagtgacatgcaacaggaaatcccagtccatgcatttgggtttggccaagaccatgatgcaagcattatgcatgctatttctgatgcatccggtggtaccttttcattcattgaatctgttggcatgatacaagatggttttgctttgtgcattggtggtcttctcagtgtagtggctcaggagcttcgcctcacagtaaggtcagcatcagctggggtgaagattgtttcaataccatcagggagacatgtgagccagatttctgatgagggccagcaaggtgtagttgatattggaaatatgtatgcagaagaggagaaacaatttctggtctacctgttagttccaccctcagcttcacatgttaagatgccattgttagatgtaacatgcgtgtacaaagatcttgcttcaaatgagttgatgcaggtgcaaggtgagagagtagagatactgagacctgaggtttgttcccctgcagaaaagacagtgtctttggaggttgatcgacagcggaacagaattgtggtggctgaaactattgcagaggcacaaaggttggctgagatgggaaacctggaaggtgcccgggcacttttggctgaacgaagagcaactgtcttagcatcagcagcagctcaagctggggatggtctcagtaatttgtttgaaactgagcttggagaagtcatggagagaatggcatctatggacttgtacgctaagtcagggcgtgcttacgctctcgcagggatgagttctcactctcgacagagggcttcagctaggggtgacacaatgacctcatgtctctttggtgcctccaaccatgtgaatttctccagcggtggcaatgccttaatgcagcagtcgggtgcctctgcttgttttggccgccctgcaggtttcagtggtgctagtttttcatccggtgccgctccaggtggaagtcaatgctcttctgcagttggtgcttttgctacacctgctatggttagaatggtagaaaaatcacagaagagcaatcaatctgggcaataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
HA2 Helicase_C OB_NTP_bind RRM_1 S1

Protein Analysis

752

Amino Acids

81.09

Weight (kDa)

6.08

Isoelectric Point (pI)

52.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000360)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11691 FvH4_3g11693 FvH4_3g11740 FvH4_3g11750 FvH4_3g11770 FvH4_3g11771 FvH4_3g11772 FvH4_3g11780
malus_domestica MD10G1239000.v1.1
prunus_persica Prupe.4G105400_v2.0.a1
pyrus_communis pycom10g20000
rosa_chinensis RchiOBHm_Chr5g0018981 RchiOBHm_Chr5g0018991 RchiOBHm_Chr5g0019101 RchiOBHm_Chr5g0019111 RchiOBHm_Chr5g0019121 RchiOBHm_Chr5g0019151 RchiOBHm_Chr5g0019161 RchiOBHm_Chr5g0019171 RchiOBHm_Chr5g0019181 RchiOBHm_Chr5g0019201
rosa_laevigata RLG00000032411 RLG00000032418 RLG00000032419 RLG00000032420 RLG00000032421 RLG00000032423 RLG00000032424 RLG00000032426
rosa_multiflora Rmu_co8475613.1_g000001 Rmu_co8503587.1_g000001 Rmu_sc0000563.1_g000023 Rmu_sc0000563.1_g000027 Rmu_sc0002142.1_g000001 Rmu_sc0002142.1_g000003 Rmu_sc0002142.1_g000004 Rmu_sc0002142.1_g000011 Rmu_sc0002142.1_g000022 Rmu_sc0002561.1_g000002 Rmu_sc0042836.1_g000001
rosa_roxburghii Rroxscaffold_1G00058790 Rroxscaffold_1G00058810 Rroxscaffold_1G00058820 Rroxscaffold_1G00058840 Rroxscaffold_1G00058850 Rroxscaffold_1G00058860 Rroxscaffold_1G00058880 Rroxscaffold_1G00058910 Rroxscaffold_1G00074090 Rroxscaffold_1G00074110 Rroxscaffold_1G00074120 Rroxscaffold_1G00074130 Rroxscaffold_1G00074150
rosa_rugosa Rorug05G0046800 Rorug05G0047200 Rorug05G0047300 Rorug05G0047400 Rorug05G0047600 Rorug05G0047600 Rorug05G0047800 Rorug05G0047900 Rorug05G0048000 Rorug05G0048100 Rorug05G0048100 Rorug05G0048200 Rorug05G0048300
rosa_samantha Rh4DG102800 Rh5AG138700 Rh5AG138900 Rh5AG139000 Rh5AG139100 Rh5AG139200 Rh5AG139400 Rh5AG139500 Rh5AG139600 Rh5BG137400 Rh5BG137600 Rh5BG137800 Rh5BG138100 Rh5BG138200 Rh5BG138300 Rh5BG138500 Rh5CG148800 Rh5CG148900 Rh5CG149200 Rh5CG149300 Rh5CG149400 Rh5CG149600 Rh5CG149700 Rh5CG150000 Rh5DG138200 Rh5DG138300 Rh5DG138500 Rh5DG138600 Rh5DG138700 Rh5DG138900 Rh5DG139000 Rh5DG139200
rosa_wichuraiana Rw5G012290 Rw5G012330 Rw5G012340 Rw5G012350 Rw5G012370 Rw5G012380 Rw5G012390 Rw5G012410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 1041, 1637, 2213
Acc36I ACCTGC 4 cut(s) 1041, 1637, 2112, 2213
Acc65I GGTACC 1 cut(s) 1314
AccB1I GGYRCC 7 cut(s) 237, 273, 1314, 1803, 2037, 2094, 2151
AccBSI CCGCTC 1 cut(s) 2157
AccI GTMKAC 1 cut(s) 1552
AccII CGCG 1 cut(s) 879
AciI CCGC 6 cut(s) 579, 750, 1733, 2065, 2114, 2155
AclWI GGATC 4 cut(s) 24, 37, 503, 516
AcoI YGGCCR 5 cut(s) 347, 1010, 1154, 1262, 2111
AcsI RAATTY 5 cut(s) 40, 909, 968, 1186, 2054
AcuI CTGAAG 2 cut(s) 919, 1984
AfaI GTAC 7 cut(s) 475, 600, 732, 827, 1316, 1617, 1938
AfiI CCNNNNNNNGG 1 cut(s) 287
AflII CTTAAG 1 cut(s) 801
AflIII ACRYGT 2 cut(s) 1466, 1580
AgsI TTSAA 8 cut(s) 126, 687, 1104, 1193, 1332, 1450, 1633, 1890
AhdI GACNNNNNGTC 1 cut(s) 2026
AhlI ACTAGT 1 cut(s) 128
AjnI CCWGG 6 cut(s) 181, 504, 619, 775, 1795, 2159
AjuI GAANNNNNNNTTGG 2 cut(s) 1882, 1914
AluBI AGCT 9 cut(s) 760, 884, 1403, 1431, 1575, 1857, 1863, 1898, 2004
AluI AGCT 9 cut(s) 760, 884, 1403, 1431, 1575, 1857, 1863, 1898, 2004
Alw21I GWGCWC 2 cut(s) 381, 794
Alw26I GTCTC 6 cut(s) 959, 1045, 1457, 1670, 1878, 2034
Alw44I GTGCAC 1 cut(s) 790
AlwI GGATC 4 cut(s) 24, 37, 503, 516
AlwNI CAGNNNCTG 2 cut(s) 165, 248
Ama87I CYCGRG 1 cut(s) 1807
AoxI GGCC 9 cut(s) 215, 347, 353, 815, 1010, 1154, 1262, 1490, 2111
ApaLI GTGCAC 1 cut(s) 790
ApoI RAATTY 5 cut(s) 40, 909, 968, 1186, 2054
ArsI GACNNNNNNTTYG 4 cut(s) 1669, 1697, 1701, 1729
Asp718I GGTACC 1 cut(s) 1314
AspLEI GCGC 1 cut(s) 879
AspS9I GGNCC 5 cut(s) 108, 623, 815, 1097, 1490
AsuC2I CCSGG 2 cut(s) 1808, 1809
AsuHPI GGTGA 5 cut(s) 552, 670, 1448, 1668, 2022
AsuII TTCGAA 1 cut(s) 230
AsuNHI GCTAGC 1 cut(s) 1131
AvaI CYCGRG 1 cut(s) 1807
AvaII GGWCC 3 cut(s) 108, 623, 1097
AxyI CCTNAGG 1 cut(s) 1681
BaeGI GKGCMC 4 cut(s) 242, 794, 1808, 1814
BalI TGGCCA 3 cut(s) 349, 1156, 1264
BamHI GGATCC 2 cut(s) 29, 508
BanI GGYRCC 7 cut(s) 237, 273, 1314, 1803, 2037, 2094, 2151
BbsI GAAGAC 2 cut(s) 925, 1371
Bbv12I GWGCWC 2 cut(s) 381, 794
BbvCI CCTCAGC 1 cut(s) 1571
BccI CCATC 8 cut(s) 132, 1002, 1041, 1146, 1347, 1463, 1781, 1863
BceAI ACGGC 1 cut(s) 997
BciT130I CCWGG 6 cut(s) 183, 506, 621, 777, 1797, 2161
BcnI CCSGG 2 cut(s) 1808, 1809
BcoDI GTCTC 6 cut(s) 959, 1045, 1457, 1670, 1878, 2034
BcuI ACTAGT 1 cut(s) 128
BfaI CTAG 8 cut(s) 129, 256, 757, 1029, 1132, 1219, 2005, 2137
BfmI CTRYAG 5 cut(s) 446, 534, 1696, 2119, 2182
BfrI CTTAAG 1 cut(s) 801
BfuAI ACCTGC 4 cut(s) 1041, 1637, 2112, 2213
BglII AGATCT 1 cut(s) 1622
BmcAI AGTACT 1 cut(s) 732
Bme1390I CCNGG 8 cut(s) 183, 506, 621, 777, 1797, 1808, 1809, 2161
Bme18I GGWCC 3 cut(s) 108, 623, 1097
BmeRI GACNNNNNGTC 1 cut(s) 2026
BmeT110I CYCGRG 1 cut(s) 1807
BmgT120I GGNCC 5 cut(s) 108, 623, 815, 1097, 1490
BmrFI CCNGG 8 cut(s) 183, 506, 621, 777, 1797, 1808, 1809, 2161
BmrI ACTGGG 2 cut(s) 1119, 1236
BmtI GCTAGC 1 cut(s) 1135
BmuI ACTGGG 2 cut(s) 1119, 1236
BpiI GAAGAC 2 cut(s) 925, 1371
BplI GAGNNNNNCTC 2 cut(s) 744, 776
BpmI CTGGAG 3 cut(s) 648, 2045, 2143
Bpu10I CCTNAGC 3 cut(s) 631, 1396, 1571
Bpu14I TTCGAA 1 cut(s) 230
BpuEI CTTGAG 3 cut(s) 292, 1065, 1843
BpuMI CCSGG 2 cut(s) 1808, 1809
BsaBI GATNNNNATC 2 cut(s) 34, 231
BsaI GGTCTC 2 cut(s) 1670, 1878
BsaJI CCNNGG 4 cut(s) 350, 619, 818, 1807
BsaWI WCCGGW 2 cut(s) 1308, 2148
Bsc4I CCNNNNNNNGG 1 cut(s) 287
Bse1I ACTGG 5 cut(s) 350, 665, 878, 1125, 1242
Bse21I CCTNAGG 1 cut(s) 1681
Bse3DI GCAATG 4 cut(s) 170, 202, 1066, 2078
Bse8I GATNNNNATC 2 cut(s) 34, 231
BseBI CCWGG 6 cut(s) 183, 506, 621, 777, 1797, 2161
BseDI CCNNGG 4 cut(s) 350, 619, 818, 1807
BseJI GATNNNNATC 2 cut(s) 34, 231
BseLI CCNNNNNNNGG 1 cut(s) 287
BseMI GCAATG 4 cut(s) 170, 202, 1066, 2078
BseNI ACTGG 5 cut(s) 350, 665, 878, 1125, 1242
BseRI GAGGAG 4 cut(s) 481, 484, 683, 1548
BseSI GKGCMC 4 cut(s) 242, 794, 1808, 1814
BseYI CCCAGC 2 cut(s) 1431, 1863
Bsh1236I CGCG 1 cut(s) 879
BshFI GGCC 9 cut(s) 217, 349, 355, 817, 1012, 1156, 1264, 1492, 2113
BshNI GGYRCC 7 cut(s) 237, 273, 1314, 1803, 2037, 2094, 2151
BsiHKAI GWGCWC 2 cut(s) 381, 794
BsiHKCI CYCGRG 1 cut(s) 1807
BsiSI CCGG 4 cut(s) 288, 1309, 1808, 2149
BslFI GGGAC 1 cut(s) 474
BslI CCNNNNNNNGG 1 cut(s) 287
BsmAI GTCTC 6 cut(s) 959, 1045, 1457, 1670, 1878, 2034
BsmFI GGGAC 1 cut(s) 474
BsnI GGCC 9 cut(s) 217, 349, 355, 817, 1012, 1156, 1264, 1492, 2113
Bso31I GGTCTC 2 cut(s) 1670, 1878
BsoBI CYCGRG 1 cut(s) 1807
Bsp119I TTCGAA 1 cut(s) 230
Bsp1286I GDGCHC 5 cut(s) 242, 381, 794, 1808, 1814
Bsp1407I TGTACA 1 cut(s) 1615
Bsp143I GATC 8 cut(s) 29, 35, 82, 361, 508, 1106, 1622, 1724
Bsp19I CCATGG 1 cut(s) 818
BspACI CCGC 6 cut(s) 579, 750, 1733, 2065, 2114, 2155
BspANI GGCC 9 cut(s) 217, 349, 355, 817, 1012, 1156, 1264, 1492, 2113
BspFNI CGCG 1 cut(s) 879
BspMAI CTGCAG 5 cut(s) 450, 538, 1700, 2123, 2186
BspMI ACCTGC 4 cut(s) 1041, 1637, 2112, 2213
BspOI GCTAGC 1 cut(s) 1135
BspPI GGATC 4 cut(s) 24, 37, 503, 516
BspQI GCTCTTC 3 cut(s) 1823, 2183, 2232
BspT104I TTCGAA 1 cut(s) 230
BspT107I GGYRCC 7 cut(s) 237, 273, 1314, 1803, 2037, 2094, 2151
BspTI CTTAAG 1 cut(s) 801
BspTNI GGTCTC 2 cut(s) 1670, 1878
BsrBI CCGCTC 1 cut(s) 2157
BsrDI GCAATG 4 cut(s) 170, 202, 1066, 2078
BsrGI TGTACA 1 cut(s) 1615
BsrI ACTGG 5 cut(s) 350, 665, 878, 1125, 1242
BssECI CCNNGG 4 cut(s) 350, 619, 818, 1807
BssMI GATC 8 cut(s) 29, 35, 82, 361, 508, 1106, 1622, 1724
BssT1I CCWWGG 2 cut(s) 350, 818
Bst2UI CCWGG 6 cut(s) 183, 506, 621, 777, 1797, 2161
Bst4CI ACNGT 7 cut(s) 387, 713, 848, 1170, 1415, 1709, 1838
Bst6I CTCTTC 4 cut(s) 1525, 1823, 2183, 2232
BstAFI CTTAAG 1 cut(s) 801
BstAPI GCANNNNNTGC 2 cut(s) 325, 2190
BstAUI TGTACA 1 cut(s) 1615
BstBI TTCGAA 1 cut(s) 230
BstC8I GCNNGC 8 cut(s) 262, 901, 1133, 1204, 1280, 1291, 1494, 1954
BstDSI CCRYGG 1 cut(s) 818
BstFNI CGCG 1 cut(s) 879
BstHHI GCGC 1 cut(s) 879
BstKTI GATC 8 cut(s) 32, 38, 85, 364, 511, 1109, 1625, 1727
BstMAI GTCTC 6 cut(s) 959, 1045, 1457, 1670, 1878, 2034
BstMBI GATC 8 cut(s) 29, 35, 82, 361, 508, 1106, 1622, 1724
BstNI CCWGG 6 cut(s) 183, 506, 621, 777, 1797, 2161
BstNSI RCATGY 5 cut(s) 1229, 1293, 1470, 1584, 1612
BstSCI CCNGG 8 cut(s) 181, 504, 619, 775, 1795, 1806, 1807, 2159
BstSFI CTRYAG 5 cut(s) 446, 534, 1696, 2119, 2182
BstSLI GKGCMC 4 cut(s) 242, 794, 1808, 1814
BstUI CGCG 1 cut(s) 879
BstV2I GAAGAC 2 cut(s) 925, 1371
BstX2I RGATCY 3 cut(s) 29, 508, 1622
BstXI CCANNNNNNTGG 1 cut(s) 1254
BstYI RGATCY 3 cut(s) 29, 508, 1622
Bsu36I CCTNAGG 1 cut(s) 1681
BsuRI GGCC 9 cut(s) 217, 349, 355, 817, 1012, 1156, 1264, 1492, 2113
BtgI CCRYGG 1 cut(s) 818
BtsIMutI CAGTG 4 cut(s) 246, 1391, 1714, 2135
BveI ACCTGC 4 cut(s) 1041, 1637, 2112, 2213
Cac8I GCNNGC 8 cut(s) 262, 901, 1133, 1204, 1280, 1291, 1494, 1954
CaiI CAGNNNCTG 2 cut(s) 165, 248
CfoI GCGC 1 cut(s) 879
Cfr13I GGNCC 5 cut(s) 108, 623, 815, 1097, 1490
Cfr9I CCCGGG 1 cut(s) 1807
Csp6I GTAC 7 cut(s) 474, 599, 731, 826, 1315, 1616, 1937
CspCI CAANNNNNGTGG 2 cut(s) 381, 416
CviQI GTAC 7 cut(s) 474, 599, 731, 826, 1315, 1616, 1937
DpnI GATC 8 cut(s) 31, 37, 84, 363, 510, 1108, 1624, 1726
DpnII GATC 8 cut(s) 29, 35, 82, 361, 508, 1106, 1622, 1724
DraI TTTAAA 1 cut(s) 71
DriI GACNNNNNGTC 1 cut(s) 2026
EaeI YGGCCR 5 cut(s) 347, 1010, 1154, 1262, 2111
Eam1104I CTCTTC 4 cut(s) 1525, 1823, 2183, 2232
Eam1105I GACNNNNNGTC 1 cut(s) 2026
EarI CTCTTC 4 cut(s) 1525, 1823, 2183, 2232
Eco130I CCWWGG 2 cut(s) 350, 818
Eco31I GGTCTC 2 cut(s) 1670, 1878
Eco47I GGWCC 3 cut(s) 108, 623, 1097
Eco57I CTGAAG 2 cut(s) 919, 1984
Eco81I CCTNAGG 1 cut(s) 1681
Eco88I CYCGRG 1 cut(s) 1807
EcoO109I RGGNCCY 2 cut(s) 623, 1097
EcoRII CCWGG 6 cut(s) 181, 504, 619, 775, 1795, 2159
EcoT14I CCWWGG 2 cut(s) 350, 818
EcoT22I ATGCAT 3 cut(s) 1253, 1291, 1307
ErhI CCWWGG 2 cut(s) 350, 818
FaqI GGGAC 1 cut(s) 474
FblI GTMKAC 1 cut(s) 1552
FspBI CTAG 8 cut(s) 129, 256, 757, 1029, 1132, 1219, 2005, 2137
GlaI GCGC 1 cut(s) 878
GsaI CCCAGC 2 cut(s) 1435, 1867
GsuI CTGGAG 3 cut(s) 648, 2045, 2143
HaeIII GGCC 9 cut(s) 217, 349, 355, 817, 1012, 1156, 1264, 1492, 2113
HapII CCGG 4 cut(s) 288, 1309, 1808, 2149
HhaI GCGC 1 cut(s) 879
Hin6I GCGC 1 cut(s) 877
HinP1I GCGC 1 cut(s) 877
HindIII AAGCTT 1 cut(s) 882
HinfI GANTC 9 cut(s) 55, 99, 227, 232, 587, 753, 807, 1038, 1332
HpaII CCGG 4 cut(s) 288, 1309, 1808, 2149
HphI GGTGA 5 cut(s) 552, 670, 1448, 1668, 2022
Hpy166II GTNNAC 4 cut(s) 179, 792, 1553, 1616
Hpy188I TCNGA 7 cut(s) 108, 650, 812, 938, 1151, 1301, 1484
Hpy188III TCNNGA 7 cut(s) 33, 309, 630, 852, 1082, 1398, 1987
Hpy8I GTNNAC 4 cut(s) 179, 792, 1553, 1616
HpyAV CCTTC 5 cut(s) 113, 123, 653, 1105, 1794
HpyCH4III ACNGT 7 cut(s) 387, 713, 848, 1170, 1415, 1709, 1838
HspAI GCGC 1 cut(s) 877
KpnI GGTACC 1 cut(s) 1318
Kzo9I GATC 8 cut(s) 29, 35, 82, 361, 508, 1106, 1622, 1724
LguI GCTCTTC 3 cut(s) 1823, 2183, 2232
LmnI GCTCC 3 cut(s) 384, 1400, 2162
MaeI CTAG 8 cut(s) 129, 256, 757, 1029, 1132, 1219, 2005, 2137
MaeIII GTNAC 8 cut(s) 131, 194, 707, 713, 842, 1221, 1604, 2010
MalI GATC 8 cut(s) 31, 37, 84, 363, 510, 1108, 1624, 1726
MbiI CCGCTC 1 cut(s) 2157
MboI GATC 8 cut(s) 29, 35, 82, 361, 508, 1106, 1622, 1724
MfeI CAATTG 1 cut(s) 1197
MflI RGATCY 3 cut(s) 29, 508, 1622
MhlI GDGCHC 5 cut(s) 242, 381, 794, 1808, 1814
MlsI TGGCCA 3 cut(s) 349, 1156, 1264
MluNI TGGCCA 3 cut(s) 349, 1156, 1264
MlyI GAGTC 3 cut(s) 108, 762, 1047
MmeI TCCRAC 1 cut(s) 2068
Mox20I TGGCCA 3 cut(s) 349, 1156, 1264
Mph1103I ATGCAT 3 cut(s) 1253, 1291, 1307
MscI TGGCCA 3 cut(s) 349, 1156, 1264
MseI TTAA 6 cut(s) 70, 802, 886, 1093, 1585, 2079
MslI CAYNNNNRTG 1 cut(s) 703
Msp20I TGGCCA 3 cut(s) 349, 1156, 1264
MspA1I CMGCKG 3 cut(s) 1431, 1733, 2065
MspCI CTTAAG 1 cut(s) 801
MspI CCGG 4 cut(s) 288, 1309, 1808, 2149
MspR9I CCNGG 8 cut(s) 183, 506, 621, 777, 1797, 1808, 1809, 2161
MunI CAATTG 1 cut(s) 1197
MvaI CCWGG 6 cut(s) 183, 506, 621, 777, 1797, 2161
MvnI CGCG 1 cut(s) 879
NciI CCSGG 2 cut(s) 1808, 1809
NcoI CCATGG 1 cut(s) 818
NdeII GATC 8 cut(s) 29, 35, 82, 361, 508, 1106, 1622, 1724
NheI GCTAGC 1 cut(s) 1131
NmuCI GTSAC 4 cut(s) 131, 707, 1221, 2010
NsiI ATGCAT 3 cut(s) 1253, 1291, 1307
NspI RCATGY 5 cut(s) 1229, 1293, 1470, 1584, 1612
NspV TTCGAA 1 cut(s) 230
PaeI GCATGC 1 cut(s) 1293
PaqCI CACCTGC 3 cut(s) 1041, 1637, 2213
PciI ACATGT 2 cut(s) 1466, 1580
PciSI GCTCTTC 3 cut(s) 1823, 2183, 2232
PfeI GAWTC 6 cut(s) 55, 227, 232, 587, 807, 1332
PflFI GACNNNGTC 1 cut(s) 1709
PleI GAGTC 3 cut(s) 107, 761, 1046
PpsI GAGTC 3 cut(s) 107, 761, 1046
PpuMI RGGWCCY 2 cut(s) 623, 1097
PscI ACATGT 2 cut(s) 1466, 1580
Psp5II RGGWCCY 2 cut(s) 623, 1097
Psp6I CCWGG 6 cut(s) 181, 504, 619, 775, 1795, 2159
PspFI CCCAGC 2 cut(s) 1431, 1863
PspGI CCWGG 6 cut(s) 181, 504, 619, 775, 1795, 2159
PspPI GGNCC 5 cut(s) 108, 623, 815, 1097, 1490
PspPPI RGGWCCY 2 cut(s) 623, 1097
PstI CTGCAG 5 cut(s) 450, 538, 1700, 2123, 2186
PstNI CAGNNNCTG 2 cut(s) 165, 248
PsuI RGATCY 3 cut(s) 29, 508, 1622
PsyI GACNNNGTC 1 cut(s) 1709
PvuII CAGCTG 1 cut(s) 1431
RsaI GTAC 7 cut(s) 475, 600, 732, 827, 1316, 1617, 1938
RsaNI GTAC 7 cut(s) 474, 599, 731, 826, 1315, 1616, 1937
RseI CAYNNNNRTG 1 cut(s) 703
SapI GCTCTTC 3 cut(s) 1823, 2183, 2232
SaqAI TTAA 6 cut(s) 70, 802, 886, 1093, 1585, 2079
Sau3AI GATC 8 cut(s) 29, 35, 82, 361, 508, 1106, 1622, 1724
Sau96I GGNCC 5 cut(s) 108, 623, 815, 1097, 1490
SbfI CCTGCAGG 1 cut(s) 2123
ScaI AGTACT 1 cut(s) 732
SchI GAGTC 3 cut(s) 108, 762, 1047
ScrFI CCNGG 8 cut(s) 183, 506, 621, 777, 1797, 1808, 1809, 2161
SdaI CCTGCAGG 1 cut(s) 2123
SduI GDGCHC 5 cut(s) 242, 381, 794, 1808, 1814
SfcI CTRYAG 5 cut(s) 446, 534, 1696, 2119, 2182
SfuI TTCGAA 1 cut(s) 230
SinI GGWCC 3 cut(s) 108, 623, 1097
SmaI CCCGGG 1 cut(s) 1809
SmiMI CAYNNNNRTG 1 cut(s) 703
SmlI CTYRAG 4 cut(s) 307, 801, 1080, 1858
SmoI CTYRAG 4 cut(s) 307, 801, 1080, 1858
SpeI ACTAGT 1 cut(s) 128
SphI GCATGC 1 cut(s) 1293
SrfI GCCCGGGC 1 cut(s) 1809
Sse8387I CCTGCAGG 1 cut(s) 2123
SsiI CCGC 6 cut(s) 579, 750, 1733, 2065, 2114, 2155
SspMI CTAG 8 cut(s) 129, 256, 757, 1029, 1132, 1219, 2005, 2137
StyD4I CCNGG 8 cut(s) 181, 504, 619, 775, 1795, 1806, 1807, 2159
StyI CCWWGG 2 cut(s) 350, 818
TaaI ACNGT 7 cut(s) 387, 713, 848, 1170, 1415, 1709, 1838
TaqI TCGA 7 cut(s) 38, 230, 1036, 1109, 1172, 1727, 1988
TatI WGTACW 2 cut(s) 730, 1615
TauI GCSGC 3 cut(s) 581, 2116, 2157
TfiI GAWTC 6 cut(s) 55, 227, 232, 587, 807, 1332
Tru1I TTAA 6 cut(s) 70, 802, 886, 1093, 1585, 2079
Tru9I TTAA 6 cut(s) 70, 802, 886, 1093, 1585, 2079
TscAI CASTG 4 cut(s) 253, 1391, 1714, 2135
TseFI GTSAC 4 cut(s) 131, 707, 1221, 2010
Tsp45I GTSAC 4 cut(s) 131, 707, 1221, 2010
TspDTI ATGAA 4 cut(s) 670, 1312, 1316, 2134
TspGWI ACGGA 1 cut(s) 777
TspMI CCCGGG 1 cut(s) 1807
TspRI CASTG 4 cut(s) 253, 1391, 1714, 2135
Tth111I GACNNNGTC 1 cut(s) 1709
Vha464I CTTAAG 1 cut(s) 801
VneI GTGCAC 1 cut(s) 790
VpaK11BI GGWCC 3 cut(s) 108, 623, 1097
XapI RAATTY 5 cut(s) 40, 909, 968, 1186, 2054
XceI RCATGY 5 cut(s) 1229, 1293, 1470, 1584, 1612
XmaI CCCGGG 1 cut(s) 1807
XmiI GTMKAC 1 cut(s) 1552
XspI CTAG 8 cut(s) 129, 256, 757, 1029, 1132, 1219, 2005, 2137
ZrmI AGTACT 1 cut(s) 732
Zsp2I ATGCAT 3 cut(s) 1253, 1291, 1307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.