Rroxscaffold_1G00074150

pre-mRNA 3'-splice site binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000360
Physical Location & Seq
Forward (+)
95082272 .. 95087292
5021 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00074150.1

Sequence Viewer

Length: 2409 bp
ATGGTGGATTGCGCGTTGCTTGGCGGCCTTGGCTACGTTGGTAGGTTTGCGCAGCTTCAGACCTCTACCACGACGGGAAGAAGTAAAGAGCTTTTGACCCAGGTGATCACTCCGAACATGTTTTCGTCTATGGCTACTGCATCTGTTCAAGGTTCTGTTGGTGGTGGGCTTGGGGATCCTGATCGAATTTTTTTTGGTGGATTCCCTTATTACTTTAAAGAAGAACAGATCAGGGAGTTGGTAGAGTCCTTCGGATCTCTTCGCAGTTTTGAACTAGTGACAGATGGAGGCAGAGGAAACAACTCAGTTGCTGCCTATTGCGTTTACCAGGATGTTTCAGTTACAGACATTGCTTGTCATGGCCTGAATGGGATTCGAATCGGTGCCCAGACACTGATTGCTAGGCGTGCTAATGATGGTGCCAACCAGCAGCCAGAACCACATTCTTCTTCTCAAGAAAATTGCAGTATTTGCTGGTGTAGCCTGACAACTGGCCAAGGCCAAGCGATCTTTACTGCTGAGTGCTCCCACCGTTTCCACTACCCTTGTATTGCCAACAATGTCCAGCATGGAAATCTTTACTGCCCTGTCTGCAGAGTAAAATGGGACAAGAATAATGTACCTTTCCAAGTTCCTCCTCCTCAACAGACCAGGATCCCTTTCAGGCAGTTCCCAATGCTGCAGCAACCCCATTGGAATTATCCTCACCTCCCATATCAGCAGCCGCATTGGAATCATCCTCAGTACCCATTTGAGCAGCAACCCCAGGACCCTCCTGAGCCACTTACCTTCTCTGATGATGAACCTCTCCAGTCATTGACCTCACCTGTTCAATCCTCTTCCCACCAGAATGTGACAGTTAAGACTTATACAGAGTGCTCTGCTATCTCTGCTGTGGAATCTAGTTCAAAGTTTCCTGTCCTTGTCAGCATCCGTGCACCACCCCTTAAGGATTCTGAGGGCCATGGTCGTACTCCCATTGACCTTGTAACAGTTCTGGATGTAAGTGGCAGCATGACTGGCTCGAAGCTTAACCTTGTCAAGCGTGCTGTCAAATTTGTCATAGAAAACTTGGGTTCTTCAGACAGACTTTCTATTGTCTCATTCTCATCAAATTCTAAGAGAGTTCTTCCTCTCCAAAGGATGACTGCTGATGGCCGTGAAAGTGCCATACTAGCCATCGAGTCTCTTAGAGCAGGTGGTGGAACTAACATTGCGGAAGCACTCAAGAAAGGAACTAAGGTCCTTGAAGATCGGAGGGAAAGGAACCCAGTTGCTAGCATCATCCTCTTATCCGATGGCCAAGATAACTACTGTCGAAGTGGAAGCCAAATTTTGAACCAATTGCCTGCTTCCATTCGTTTTAGTGACATGGAACAGGAAATCCCAGTCCATGCATTTGGGTTTGGCCAAGACCATGATGCAAGCATTATGCATGCTATTTCTGATGCATCTGGTGGTACCTTTTCATTCATTGAATCAGTTGGCATGATACAAGATGGTTTTGCTTTGTGCATTGGTGGTCTTCTCAGTGTAGTGGCTCAGGAACTTCGCCTCACAGTAAGGTCAGCATCAGCTGGGGTGAAGATTGTTTCAATACCATCAGGGAGACATTTCAACCAGATTTCTGATGAGGGCCAGCAAGGTGCAGTTGATATTGGAAATATGTATGCAGAAGAGGAGAAACAATTTCTGGTCTACCTGTTAGTTCCACCCTCAGCTTCACATGTTAAGATGCCATTGTTAGATGTAACATGCGTGTACAAAGATCTTGCTTCAAATGAGTTGATGCAGGTGCAAGGTGAGAGAGTAGAGATACTGAGACCTGAGGTTTGTTCCCCTGCAGAAAAGGCTGTGTCTTTGGAGGTTGATCGACAGCGGAATAGAATTGTGGTGGCTGAAACTATTGCAGAGGCACAAAGGTTGGCTGAGATGGGAAATCTGGAAGGTGCACGGGCACTGTTGGCTGAACGAAGAGCAACTGTCTTAGCATCAGCAGCAGCTCAAGCTGGGGATGGTCTCAGTAATTTGTTTGAAACTGAGCTTAGAGAAGTCATGGAGAGAATGGCATCTATGGACTTGTACGCTAACTCAGGGCGTGCTTACGCGCTCGCAGGGATGAGTTCTCATTCTCGACAGAGGGCTTCAGCTAGGGGTGACACAATAACCTCATGTCTCTTTGGTGCCTCCAACCGTGTGAATTTCTCTAGCGGTGGCAATGCCTTGATGCAGCAGTCGGGTGCCTCTGCTCTTTTTGGTGCCCCTGCAGGTTTCGGTGGTGCTAGTTTTTCATCCGGTGCCGCCGCCCCAGGTGGAAGTCAATGGTCTTCTGCAGTTGGTGCTTTTGAAACACCTGCTATGCTTAGAATGGTAGAAAAATCACAGAAGAGCAATCAATCTGGGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
HA2 Helicase_C OB_NTP_bind RRM_1 S1

Protein Analysis

802

Amino Acids

86.52

Weight (kDa)

5.92

Isoelectric Point (pI)

50.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 63 - 131 5.4e-07 RNA recognition motif
zf-RING_11 PF17123 154 - 184 2.6e-09 RING-like zinc finger
zf-RING_2 PF13639 154 - 199 8.1e-07 Ring finger domain
VWA_CoxE PF05762 324 - 441 3.2e-07 VWA domain containing CoxE-like protein
VWA PF00092 328 - 492 4.2e-24 von Willebrand factor type A domain
VWA_3 PF13768 328 - 491 6.2e-19 von Willebrand factor type A domain
VWA_2 PF13519 329 - 435 2.2e-19 von Willebrand factor type A domain
WAV3_C PF25243 623 - 689 9.7e-06 E3 ubiquitin-protein ligase WAV3-like, C-terminal domain
Vwaint PF14624 679 - 721 6.5e-11 VWA / Hh protein intein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000360)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11691 FvH4_3g11693 FvH4_3g11740 FvH4_3g11750 FvH4_3g11770 FvH4_3g11771 FvH4_3g11772 FvH4_3g11780
malus_domestica MD10G1239000.v1.1
prunus_persica Prupe.4G105400_v2.0.a1
pyrus_communis pycom10g20000
rosa_chinensis RchiOBHm_Chr5g0018981 RchiOBHm_Chr5g0018991 RchiOBHm_Chr5g0019101 RchiOBHm_Chr5g0019111 RchiOBHm_Chr5g0019121 RchiOBHm_Chr5g0019151 RchiOBHm_Chr5g0019161 RchiOBHm_Chr5g0019171 RchiOBHm_Chr5g0019181 RchiOBHm_Chr5g0019201
rosa_laevigata RLG00000032411 RLG00000032418 RLG00000032419 RLG00000032420 RLG00000032421 RLG00000032423 RLG00000032424 RLG00000032426
rosa_multiflora Rmu_co8475613.1_g000001 Rmu_co8503587.1_g000001 Rmu_sc0000563.1_g000023 Rmu_sc0000563.1_g000027 Rmu_sc0002142.1_g000001 Rmu_sc0002142.1_g000003 Rmu_sc0002142.1_g000004 Rmu_sc0002142.1_g000011 Rmu_sc0002142.1_g000022 Rmu_sc0002561.1_g000002 Rmu_sc0042836.1_g000001
rosa_roxburghii Rroxscaffold_1G00058790 Rroxscaffold_1G00058810 Rroxscaffold_1G00058820 Rroxscaffold_1G00058840 Rroxscaffold_1G00058850 Rroxscaffold_1G00058860 Rroxscaffold_1G00058880 Rroxscaffold_1G00058910 Rroxscaffold_1G00074090 Rroxscaffold_1G00074110 Rroxscaffold_1G00074120 Rroxscaffold_1G00074130 Rroxscaffold_1G00074150
rosa_rugosa Rorug05G0046800 Rorug05G0047200 Rorug05G0047300 Rorug05G0047400 Rorug05G0047600 Rorug05G0047600 Rorug05G0047800 Rorug05G0047900 Rorug05G0048000 Rorug05G0048100 Rorug05G0048100 Rorug05G0048200 Rorug05G0048300
rosa_samantha Rh4DG102800 Rh5AG138700 Rh5AG138900 Rh5AG139000 Rh5AG139100 Rh5AG139200 Rh5AG139400 Rh5AG139500 Rh5AG139600 Rh5BG137400 Rh5BG137600 Rh5BG137800 Rh5BG138100 Rh5BG138200 Rh5BG138300 Rh5BG138500 Rh5CG148800 Rh5CG148900 Rh5CG149200 Rh5CG149300 Rh5CG149400 Rh5CG149600 Rh5CG149700 Rh5CG150000 Rh5DG138200 Rh5DG138300 Rh5DG138500 Rh5DG138600 Rh5DG138700 Rh5DG138900 Rh5DG139000 Rh5DG139200
rosa_wichuraiana Rw5G012290 Rw5G012330 Rw5G012340 Rw5G012350 Rw5G012370 Rw5G012380 Rw5G012390 Rw5G012410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 1187, 1783, 2362
Acc16I TGCGCA 1 cut(s) 51
Acc36I ACCTGC 4 cut(s) 1187, 1783, 2258, 2362
Acc65I GGTACC 1 cut(s) 1460
AccB1I GGYRCC 7 cut(s) 383, 419, 1460, 2183, 2240, 2258, 2297
AccI GTMKAC 1 cut(s) 1698
AccII CGCG 2 cut(s) 14, 2108
AciI CCGC 7 cut(s) 24, 725, 1217, 1879, 2211, 2301, 2304
AclWI GGATC 5 cut(s) 170, 183, 262, 649, 662
AcoI YGGCCR 4 cut(s) 493, 1156, 1300, 1408
AcsI RAATTY 5 cut(s) 186, 1055, 1114, 1332, 2200
AcuI CTGAAG 3 cut(s) 41, 1065, 2130
AfaI GTAC 6 cut(s) 621, 746, 973, 1462, 1763, 2084
AflII CTTAAG 1 cut(s) 947
AflIII ACRYGT 2 cut(s) 117, 1726
AhlI ACTAGT 1 cut(s) 274
AjnI CCWGG 5 cut(s) 99, 327, 650, 765, 2308
AloI GAACNNNNNNTCC 2 cut(s) 1368, 1400
AluBI AGCT 9 cut(s) 55, 91, 1030, 1577, 1721, 2003, 2009, 2044, 2150
AluI AGCT 9 cut(s) 55, 91, 1030, 1577, 1721, 2003, 2009, 2044, 2150
Alw21I GWGCWC 4 cut(s) 527, 881, 940, 1954
Alw26I GTCTC 6 cut(s) 1105, 1191, 1603, 1816, 2024, 2180
Alw44I GTGCAC 2 cut(s) 936, 1950
AlwI GGATC 5 cut(s) 170, 183, 262, 649, 662
AlwNI CAGNNNCTG 2 cut(s) 311, 394
AoxI GGCC 9 cut(s) 25, 361, 493, 499, 961, 1156, 1300, 1408, 1636
ApaLI GTGCAC 2 cut(s) 936, 1950
ApoI RAATTY 5 cut(s) 186, 1055, 1114, 1332, 2200
ArsI GACNNNNNNTTYG 2 cut(s) 1815, 1847
Asp718I GGTACC 1 cut(s) 1460
AspLEI GCGC 3 cut(s) 14, 52, 2110
AspS9I GGNCC 4 cut(s) 769, 961, 1243, 1636
AsuHPI GGTGA 6 cut(s) 115, 698, 816, 1594, 1814, 2168
AsuII TTCGAA 1 cut(s) 376
AsuNHI GCTAGC 1 cut(s) 1277
AvaII GGWCC 2 cut(s) 769, 1243
AxyI CCTNAGG 1 cut(s) 1827
BaeGI GKGCMC 5 cut(s) 388, 940, 1954, 1960, 2263
BalI TGGCCA 3 cut(s) 495, 1302, 1410
BamHI GGATCC 2 cut(s) 175, 654
BanI GGYRCC 7 cut(s) 383, 419, 1460, 2183, 2240, 2258, 2297
BbsI GAAGAC 2 cut(s) 1517, 2319
Bbv12I GWGCWC 4 cut(s) 527, 881, 940, 1954
BbvCI CCTCAGC 1 cut(s) 1717
BccI CCATC 9 cut(s) 278, 410, 1148, 1187, 1292, 1493, 1609, 1927, 2009
BceAI ACGGC 1 cut(s) 1143
BciT130I CCWGG 5 cut(s) 101, 329, 652, 767, 2310
BclI TGATCA 1 cut(s) 105
BcoDI GTCTC 6 cut(s) 1105, 1191, 1603, 1816, 2024, 2180
BcuI ACTAGT 1 cut(s) 274
BfaI CTAG 8 cut(s) 275, 402, 903, 1175, 1278, 2151, 2208, 2283
BfmI CTRYAG 5 cut(s) 592, 680, 1842, 2265, 2331
BfrI CTTAAG 1 cut(s) 947
BfuAI ACCTGC 4 cut(s) 1187, 1783, 2258, 2362
BglII AGATCT 1 cut(s) 1768
Bme1390I CCNGG 5 cut(s) 101, 329, 652, 767, 2310
Bme18I GGWCC 2 cut(s) 769, 1243
BmgT120I GGNCC 4 cut(s) 769, 961, 1243, 1636
BmrFI CCNGG 5 cut(s) 101, 329, 652, 767, 2310
BmrI ACTGGG 2 cut(s) 1265, 1382
BmtI GCTAGC 1 cut(s) 1281
BmuI ACTGGG 2 cut(s) 1265, 1382
BpiI GAAGAC 2 cut(s) 1517, 2319
BpmI CTGGAG 1 cut(s) 794
Bpu10I CCTNAGC 3 cut(s) 777, 1542, 1717
Bpu14I TTCGAA 1 cut(s) 376
BpuEI CTTGAG 3 cut(s) 438, 1211, 1989
BsaBI GATNNNNATC 2 cut(s) 180, 377
BsaI GGTCTC 2 cut(s) 1816, 2024
BsaJI CCNNGG 6 cut(s) 28, 99, 496, 765, 964, 2308
BsaWI WCCGGW 1 cut(s) 2294
Bse1I ACTGG 5 cut(s) 496, 811, 1024, 1271, 1388
Bse21I CCTNAGG 1 cut(s) 1827
Bse3DI GCAATG 3 cut(s) 348, 1212, 2224
Bse8I GATNNNNATC 2 cut(s) 180, 377
BseBI CCWGG 5 cut(s) 101, 329, 652, 767, 2310
BseDI CCNNGG 6 cut(s) 28, 99, 496, 765, 964, 2308
BseGI GGATG 9 cut(s) 337, 736, 930, 1006, 1149, 1284, 2020, 2124, 2291
BseJI GATNNNNATC 2 cut(s) 180, 377
BseMI GCAATG 3 cut(s) 348, 1212, 2224
BseNI ACTGG 5 cut(s) 496, 811, 1024, 1271, 1388
BseRI GAGGAG 3 cut(s) 627, 630, 1694
BseSI GKGCMC 5 cut(s) 388, 940, 1954, 1960, 2263
BseYI CCCAGC 2 cut(s) 1577, 2009
BsgI GTGCAG 1 cut(s) 1668
Bsh1236I CGCG 2 cut(s) 14, 2108
BshFI GGCC 9 cut(s) 27, 363, 495, 501, 963, 1158, 1302, 1410, 1638
BshNI GGYRCC 7 cut(s) 383, 419, 1460, 2183, 2240, 2258, 2297
BsiHKAI GWGCWC 4 cut(s) 527, 881, 940, 1954
BsiSI CCGG 1 cut(s) 2295
BslFI GGGAC 1 cut(s) 620
BsmAI GTCTC 6 cut(s) 1105, 1191, 1603, 1816, 2024, 2180
BsmFI GGGAC 1 cut(s) 620
BsnI GGCC 9 cut(s) 27, 363, 495, 501, 963, 1158, 1302, 1410, 1638
Bso31I GGTCTC 2 cut(s) 1816, 2024
Bsp119I TTCGAA 1 cut(s) 376
Bsp1286I GDGCHC 7 cut(s) 388, 527, 881, 940, 1954, 1960, 2263
Bsp1407I TGTACA 1 cut(s) 1761
Bsp19I CCATGG 1 cut(s) 964
BspACI CCGC 7 cut(s) 24, 725, 1217, 1879, 2211, 2301, 2304
BspANI GGCC 9 cut(s) 27, 363, 495, 501, 963, 1158, 1302, 1410, 1638
BspFNI CGCG 2 cut(s) 14, 2108
BspMAI CTGCAG 5 cut(s) 596, 684, 1846, 2269, 2335
BspMI ACCTGC 4 cut(s) 1187, 1783, 2258, 2362
BspOI GCTAGC 1 cut(s) 1281
BspPI GGATC 5 cut(s) 170, 183, 262, 649, 662
BspQI GCTCTTC 2 cut(s) 1969, 2381
BspT104I TTCGAA 1 cut(s) 376
BspT107I GGYRCC 7 cut(s) 383, 419, 1460, 2183, 2240, 2258, 2297
BspTI CTTAAG 1 cut(s) 947
BspTNI GGTCTC 2 cut(s) 1816, 2024
BsrDI GCAATG 3 cut(s) 348, 1212, 2224
BsrGI TGTACA 1 cut(s) 1761
BsrI ACTGG 5 cut(s) 496, 811, 1024, 1271, 1388
BssECI CCNNGG 6 cut(s) 28, 99, 496, 765, 964, 2308
BssT1I CCWWGG 3 cut(s) 28, 496, 964
Bst2UI CCWGG 5 cut(s) 101, 329, 652, 767, 2310
Bst4CI ACNGT 8 cut(s) 533, 859, 994, 1316, 1561, 1962, 1984, 2195
Bst6I CTCTTC 5 cut(s) 264, 844, 1671, 1969, 2381
BstAFI CTTAAG 1 cut(s) 947
BstAPI GCANNNNNTGC 2 cut(s) 471, 2339
BstAUI TGTACA 1 cut(s) 1761
BstBI TTCGAA 1 cut(s) 376
BstC8I GCNNGC 9 cut(s) 408, 1047, 1279, 1350, 1426, 1437, 1640, 2100, 2112
BstDSI CCRYGG 1 cut(s) 964
BstF5I GGATG 9 cut(s) 337, 736, 930, 1006, 1149, 1284, 2020, 2124, 2291
BstFNI CGCG 2 cut(s) 14, 2108
BstHHI GCGC 3 cut(s) 14, 52, 2110
BstMAI GTCTC 6 cut(s) 1105, 1191, 1603, 1816, 2024, 2180
BstNI CCWGG 5 cut(s) 101, 329, 652, 767, 2310
BstNSI RCATGY 4 cut(s) 121, 1439, 1730, 1758
BstSCI CCNGG 5 cut(s) 99, 327, 650, 765, 2308
BstSFI CTRYAG 5 cut(s) 592, 680, 1842, 2265, 2331
BstSLI GKGCMC 5 cut(s) 388, 940, 1954, 1960, 2263
BstUI CGCG 2 cut(s) 14, 2108
BstV2I GAAGAC 2 cut(s) 1517, 2319
BstX2I RGATCY 4 cut(s) 175, 254, 654, 1768
BstXI CCANNNNNNTGG 1 cut(s) 1400
BstYI RGATCY 4 cut(s) 175, 254, 654, 1768
Bsu36I CCTNAGG 1 cut(s) 1827
BsuRI GGCC 9 cut(s) 27, 363, 495, 501, 963, 1158, 1302, 1410, 1638
BtgI CCRYGG 1 cut(s) 964
BtsCI GGATG 9 cut(s) 337, 736, 930, 1006, 1149, 1284, 2020, 2124, 2291
BtsIMutI CAGTG 3 cut(s) 392, 1537, 1958
BveI ACCTGC 4 cut(s) 1187, 1783, 2258, 2362
Cac8I GCNNGC 9 cut(s) 408, 1047, 1279, 1350, 1426, 1437, 1640, 2100, 2112
CaiI CAGNNNCTG 2 cut(s) 311, 394
CfoI GCGC 3 cut(s) 14, 52, 2110
Cfr13I GGNCC 4 cut(s) 769, 961, 1243, 1636
Csp6I GTAC 6 cut(s) 620, 745, 972, 1461, 1762, 2083
CspCI CAANNNNNGTGG 2 cut(s) 527, 562
CviQI GTAC 6 cut(s) 620, 745, 972, 1461, 1762, 2083
DraI TTTAAA 1 cut(s) 217
EaeI YGGCCR 4 cut(s) 493, 1156, 1300, 1408
Eam1104I CTCTTC 5 cut(s) 264, 844, 1671, 1969, 2381
EarI CTCTTC 5 cut(s) 264, 844, 1671, 1969, 2381
Eco130I CCWWGG 3 cut(s) 28, 496, 964
Eco31I GGTCTC 2 cut(s) 1816, 2024
Eco47I GGWCC 2 cut(s) 769, 1243
Eco57I CTGAAG 3 cut(s) 41, 1065, 2130
Eco81I CCTNAGG 1 cut(s) 1827
EcoO109I RGGNCCY 2 cut(s) 769, 1243
EcoRII CCWGG 5 cut(s) 99, 327, 650, 765, 2308
EcoT14I CCWWGG 3 cut(s) 28, 496, 964
EcoT22I ATGCAT 3 cut(s) 1399, 1437, 1453
ErhI CCWWGG 3 cut(s) 28, 496, 964
FaqI GGGAC 1 cut(s) 620
FbaI TGATCA 1 cut(s) 105
FblI GTMKAC 1 cut(s) 1698
FokI GGATG 9 cut(s) 344, 723, 917, 1013, 1156, 1271, 2027, 2131, 2278
FspBI CTAG 8 cut(s) 275, 402, 903, 1175, 1278, 2151, 2208, 2283
FspI TGCGCA 1 cut(s) 51
GlaI GCGC 3 cut(s) 13, 51, 2109
GsaI CCCAGC 2 cut(s) 1581, 2013
GsuI CTGGAG 1 cut(s) 794
HaeIII GGCC 9 cut(s) 27, 363, 495, 501, 963, 1158, 1302, 1410, 1638
HapII CCGG 1 cut(s) 2295
HhaI GCGC 3 cut(s) 14, 52, 2110
Hin6I GCGC 3 cut(s) 12, 50, 2108
HinP1I GCGC 3 cut(s) 12, 50, 2108
HindIII AAGCTT 1 cut(s) 1028
HinfI GANTC 9 cut(s) 201, 245, 373, 378, 733, 899, 953, 1184, 1478
HpaII CCGG 1 cut(s) 2295
HphI GGTGA 6 cut(s) 115, 698, 816, 1594, 1814, 2168
Hpy166II GTNNAC 5 cut(s) 325, 938, 1699, 1762, 1952
Hpy188III TCNNGA 8 cut(s) 179, 455, 776, 998, 1228, 1544, 1943, 2133
Hpy8I GTNNAC 5 cut(s) 325, 938, 1699, 1762, 1952
Hpy99I CGWCG 1 cut(s) 76
HpyAV CCTTC 3 cut(s) 259, 799, 1940
HpyCH4III ACNGT 8 cut(s) 533, 859, 994, 1316, 1561, 1962, 1984, 2195
HpyCH4IV ACGT 1 cut(s) 36
HpySE526I ACGT 1 cut(s) 36
HspAI GCGC 3 cut(s) 12, 50, 2108
KpnI GGTACC 1 cut(s) 1464
Ksp22I TGATCA 1 cut(s) 105
LguI GCTCTTC 2 cut(s) 1969, 2381
LmnI GCTCC 1 cut(s) 530
MaeI CTAG 8 cut(s) 275, 402, 903, 1175, 1278, 2151, 2208, 2283
MaeII ACGT 1 cut(s) 36
MaeIII GTNAC 7 cut(s) 277, 340, 853, 988, 1367, 1750, 2156
MfeI CAATTG 1 cut(s) 1343
MflI RGATCY 4 cut(s) 175, 254, 654, 1768
MhlI GDGCHC 7 cut(s) 388, 527, 881, 940, 1954, 1960, 2263
MlsI TGGCCA 3 cut(s) 495, 1302, 1410
MluNI TGGCCA 3 cut(s) 495, 1302, 1410
MlyI GAGTC 2 cut(s) 254, 1193
MmeI TCCRAC 1 cut(s) 2214
Mox20I TGGCCA 3 cut(s) 495, 1302, 1410
Mph1103I ATGCAT 3 cut(s) 1399, 1437, 1453
MscI TGGCCA 3 cut(s) 495, 1302, 1410
MseI TTAA 5 cut(s) 216, 861, 948, 1032, 1731
MslI CAYNNNNRTG 1 cut(s) 849
Msp20I TGGCCA 3 cut(s) 495, 1302, 1410
MspA1I CMGCKG 2 cut(s) 1577, 1879
MspCI CTTAAG 1 cut(s) 947
MspI CCGG 1 cut(s) 2295
MspR9I CCNGG 5 cut(s) 101, 329, 652, 767, 2310
MunI CAATTG 1 cut(s) 1343
MvaI CCWGG 5 cut(s) 101, 329, 652, 767, 2310
MvnI CGCG 2 cut(s) 14, 2108
NcoI CCATGG 1 cut(s) 964
NheI GCTAGC 1 cut(s) 1277
NmuCI GTSAC 4 cut(s) 277, 853, 1367, 2156
NsbI TGCGCA 1 cut(s) 51
NsiI ATGCAT 3 cut(s) 1399, 1437, 1453
NspI RCATGY 4 cut(s) 121, 1439, 1730, 1758
NspV TTCGAA 1 cut(s) 376
PaeI GCATGC 1 cut(s) 1439
PaqCI CACCTGC 3 cut(s) 1187, 1783, 2362
PciI ACATGT 2 cut(s) 117, 1726
PciSI GCTCTTC 2 cut(s) 1969, 2381
PfeI GAWTC 7 cut(s) 201, 373, 378, 733, 899, 953, 1478
PleI GAGTC 2 cut(s) 253, 1192
PpsI GAGTC 2 cut(s) 253, 1192
PpuMI RGGWCCY 2 cut(s) 769, 1243
PscI ACATGT 2 cut(s) 117, 1726
Psp5II RGGWCCY 2 cut(s) 769, 1243
Psp6I CCWGG 5 cut(s) 99, 327, 650, 765, 2308
PspFI CCCAGC 2 cut(s) 1577, 2009
PspGI CCWGG 5 cut(s) 99, 327, 650, 765, 2308
PspPI GGNCC 4 cut(s) 769, 961, 1243, 1636
PspPPI RGGWCCY 2 cut(s) 769, 1243
PstI CTGCAG 5 cut(s) 596, 684, 1846, 2269, 2335
PstNI CAGNNNCTG 2 cut(s) 311, 394
PsuI RGATCY 4 cut(s) 175, 254, 654, 1768
PvuII CAGCTG 1 cut(s) 1577
RsaI GTAC 6 cut(s) 621, 746, 973, 1462, 1763, 2084
RsaNI GTAC 6 cut(s) 620, 745, 972, 1461, 1762, 2083
RseI CAYNNNNRTG 1 cut(s) 849
SapI GCTCTTC 2 cut(s) 1969, 2381
SaqAI TTAA 5 cut(s) 216, 861, 948, 1032, 1731
Sau96I GGNCC 4 cut(s) 769, 961, 1243, 1636
SbfI CCTGCAGG 1 cut(s) 2269
SchI GAGTC 2 cut(s) 254, 1193
ScrFI CCNGG 5 cut(s) 101, 329, 652, 767, 2310
SdaI CCTGCAGG 1 cut(s) 2269
SduI GDGCHC 7 cut(s) 388, 527, 881, 940, 1954, 1960, 2263
SfcI CTRYAG 5 cut(s) 592, 680, 1842, 2265, 2331
SfuI TTCGAA 1 cut(s) 376
SinI GGWCC 2 cut(s) 769, 1243
SmiMI CAYNNNNRTG 1 cut(s) 849
SmlI CTYRAG 4 cut(s) 453, 947, 1226, 2004
SmoI CTYRAG 4 cut(s) 453, 947, 1226, 2004
SpeI ACTAGT 1 cut(s) 274
SphI GCATGC 1 cut(s) 1439
Sse8387I CCTGCAGG 1 cut(s) 2269
SsiI CCGC 7 cut(s) 24, 725, 1217, 1879, 2211, 2301, 2304
SspMI CTAG 8 cut(s) 275, 402, 903, 1175, 1278, 2151, 2208, 2283
StyD4I CCNGG 5 cut(s) 99, 327, 650, 765, 2308
StyI CCWWGG 3 cut(s) 28, 496, 964
TaaI ACNGT 8 cut(s) 533, 859, 994, 1316, 1561, 1962, 1984, 2195
TaiI ACGT 1 cut(s) 39
TaqI TCGA 7 cut(s) 184, 376, 1025, 1182, 1318, 1873, 2134
TatI WGTACW 1 cut(s) 1761
TauI GCSGC 4 cut(s) 27, 727, 2303, 2306
TfiI GAWTC 7 cut(s) 201, 373, 378, 733, 899, 953, 1478
Tru1I TTAA 5 cut(s) 216, 861, 948, 1032, 1731
Tru9I TTAA 5 cut(s) 216, 861, 948, 1032, 1731
TscAI CASTG 3 cut(s) 399, 1537, 1965
TseFI GTSAC 4 cut(s) 277, 853, 1367, 2156
Tsp45I GTSAC 4 cut(s) 277, 853, 1367, 2156
TspDTI ATGAA 4 cut(s) 816, 1458, 1462, 2280
TspGWI ACGGA 1 cut(s) 923
TspRI CASTG 3 cut(s) 399, 1537, 1965
Vha464I CTTAAG 1 cut(s) 947
VneI GTGCAC 2 cut(s) 936, 1950
VpaK11BI GGWCC 2 cut(s) 769, 1243
XapI RAATTY 5 cut(s) 186, 1055, 1114, 1332, 2200
XceI RCATGY 4 cut(s) 121, 1439, 1730, 1758
XmiI GTMKAC 1 cut(s) 1698
XspI CTAG 8 cut(s) 275, 402, 903, 1175, 1278, 2151, 2208, 2283
Zsp2I ATGCAT 3 cut(s) 1399, 1437, 1453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.