Prupe.4G105400_v2.0.a1

VWA / Hh protein intein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000360
Physical Location & Seq
Forward (+)
5443650 .. 5447532
3883 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G105400.1

Sequence Viewer

Length: 2040 bp
ATGGCCGCTACGTGGCGGAAGCTGAAGAAGTCTCTGAGTCACAAGTTCTCATTAAACTCGTCCGATTCATCCAACTCGTCGTCTCGGCCGCCTCGTCCTCCTCCCCCCGAAATCTCGGCAACCGTCGGGCCCTCTCCCTCTTCTCGCTCCTCCTCCTCCTCCTCCTCCGCCATCTCCAAGCTCTCTCGCAGCTTCAGCTCTCGCCTCTCCAAGAAAAATTGCGCCATTTGCCTGAGTAATGTGAGAGCAGGGCAAGGCCAAGCCATCTTCACTGCTGAGTGCTCCCACTCTTTCCATTTCGTTTGTATTGCCAACAACGTCAAACATGGAAACCTTTGCTGCCCAATCTGCCGATCCAAGTGGAATGCTGTACCTTTCCAAGCTCCCACCAATGCTGCAGATCTTCAACAAAATAACTTGGGTCAGATGCATACCAATGTCTACCATCGTCACCAGTCTCACCTCCCATTGCAGCCCCGGGTCCATCCCGAGCCGCTTAGCTTCTCAGATGATGAACCTCTCCCATCCACCTCACCTACTCGACTCTCTGGCCCCCAAACTGTAGCAATCAAGAGTTATTCAGAGTACTCTGCTATCTCTGTTGCAGAATCTCGCCCTACATTTCCTGTTCTTGTCAGCATCCGAGCACCACCTCTTCAAGATGCTAATGGCCATGGCCGTACCCCCATTGACCTTGTGACAGTTCTGGACGTAAGTGGCAGCATGTTTGGCACAAAGCTTGCCCTTCTCAAGAGTGCTGTCAAATTTGTCATACAAAACTTGGGGCCTTATGACCGACTTTCCATAGTTTCGTTCTCAACTACTCCTAAAAGGGTTTTTCCTCTGCGAAGAATGACTGTTGATGGGCGTGAAAGCGCCATTTTAGCAGTCGAGTCTCTTAGAGCAAATGGTGGAACTGACATTGTGGGAGGACTCAAGAAAGGAGTTCAGGTCCTTGAAGAACGCCGTGAAAGGAATCCGGTTTCTAGCATCATCCTCTTATCCGATGGCAGAGACAATTGCAACTATGGCTCACAGACTACTTCAAGTGCAAACCAGGTGTCTGAGTTTTTGAACCAATTGCCAGCTTCGGTACGGTGTAGCAATATTCCAGTCCACGCATTTGGATTTGGCACTGACCATGATGCCACTACCATGCATGCTATTTCTGATTCATCTGGTGGCACCTTTTCCTTCATTGAATCAGTTGCTATGATACAAGATGCCTTTGCTATGTGCATTGGTGGTCTTCTCAGTGTTGTGGCTCAAGAACTTCGACTCACAGTTAGGTCAGCATCACAGGGAGTGCAGATTGTGTCAATACCATCAGGGAGACATATGAGTGAGATTTCTGATGAGTCTCAACAAGGTGTTGTTGACGTTGGAAATATGTACGCAGAAGAGGAGAAACAATTTCTGGTGTACCTTTCGGTTCCAATATCCACAGGAACTAGAAATAGGGAAGGCGTGAAAAAGACACCATTGTTGGATGTATCATGCTTGTACAAAGATCTAGGCTCAAATGAGTTAATCCAGGTGGTAGGTGAGAGGGTAGAGATACTGAGACCTGAAGCTTGGTCCCCTGCAGACGAGGCAGTGTGTTTGGAGGTTGATCGGCAGAGGAATAGAATTTTGGTAGCCGAAGGTATCACAGAAGCACAAGTGATGGCTGAAATGGGAAATCTAGAAGGTGCACAGGCCATTTTGGCACAACGTAGATCAACTCTCTTAACAACAGCAGCAGCACAAGCTGGGGATGGTCTCTGTAATTGGCTTGAAGCTGAGCTTAGAGAAATCAGGGATAGAATGGCAAGTATGGAATCATATACACAGACCGGGCGCGCTTATGTTCTTTCTGGGTTGAGCTCACATTCATGGCAGAGAGCCACAACTAGGGGTGACATGACAACCCAGATTCTCACGATGAGCGAAGGTGGAACCTCACGCACAAGCGTTGGCACAACCGTCGGCTATGAAACACCGACGATGGTTACCATGGTAAGGATGTCACAGAATCTTGGGGTTAATCCTGCAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VWA VWA_3 Vwaint zf-RING_11 zf-RING_2

Protein Analysis

680

Amino Acids

73.33

Weight (kDa)

8.36

Isoelectric Point (pI)

51.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000360)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11691 FvH4_3g11693 FvH4_3g11740 FvH4_3g11750 FvH4_3g11770 FvH4_3g11771 FvH4_3g11772 FvH4_3g11780
malus_domestica MD10G1239000.v1.1
prunus_persica Prupe.4G105400_v2.0.a1
pyrus_communis pycom10g20000
rosa_chinensis RchiOBHm_Chr5g0018981 RchiOBHm_Chr5g0018991 RchiOBHm_Chr5g0019101 RchiOBHm_Chr5g0019111 RchiOBHm_Chr5g0019121 RchiOBHm_Chr5g0019151 RchiOBHm_Chr5g0019161 RchiOBHm_Chr5g0019171 RchiOBHm_Chr5g0019181 RchiOBHm_Chr5g0019201
rosa_laevigata RLG00000032411 RLG00000032418 RLG00000032419 RLG00000032420 RLG00000032421 RLG00000032423 RLG00000032424 RLG00000032426
rosa_multiflora Rmu_co8475613.1_g000001 Rmu_co8503587.1_g000001 Rmu_sc0000563.1_g000023 Rmu_sc0000563.1_g000027 Rmu_sc0002142.1_g000001 Rmu_sc0002142.1_g000003 Rmu_sc0002142.1_g000004 Rmu_sc0002142.1_g000011 Rmu_sc0002142.1_g000022 Rmu_sc0002561.1_g000002 Rmu_sc0042836.1_g000001
rosa_roxburghii Rroxscaffold_1G00058790 Rroxscaffold_1G00058810 Rroxscaffold_1G00058820 Rroxscaffold_1G00058840 Rroxscaffold_1G00058850 Rroxscaffold_1G00058860 Rroxscaffold_1G00058880 Rroxscaffold_1G00058910 Rroxscaffold_1G00074090 Rroxscaffold_1G00074110 Rroxscaffold_1G00074120 Rroxscaffold_1G00074130 Rroxscaffold_1G00074150
rosa_rugosa Rorug05G0046800 Rorug05G0047200 Rorug05G0047300 Rorug05G0047400 Rorug05G0047600 Rorug05G0047600 Rorug05G0047800 Rorug05G0047900 Rorug05G0048000 Rorug05G0048100 Rorug05G0048100 Rorug05G0048200 Rorug05G0048300
rosa_samantha Rh4DG102800 Rh5AG138700 Rh5AG138900 Rh5AG139000 Rh5AG139100 Rh5AG139200 Rh5AG139400 Rh5AG139500 Rh5AG139600 Rh5BG137400 Rh5BG137600 Rh5BG137800 Rh5BG138100 Rh5BG138200 Rh5BG138300 Rh5BG138500 Rh5CG148800 Rh5CG148900 Rh5CG149200 Rh5CG149300 Rh5CG149400 Rh5CG149600 Rh5CG149700 Rh5CG150000 Rh5DG138200 Rh5DG138300 Rh5DG138500 Rh5DG138600 Rh5DG138700 Rh5DG138900 Rh5DG139000 Rh5DG139200
rosa_wichuraiana Rw5G012290 Rw5G012330 Rw5G012340 Rw5G012350 Rw5G012370 Rw5G012380 Rw5G012390 Rw5G012410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1184
AccI GTMKAC 1 cut(s) 441
AccII CGCG 1 cut(s) 1842
AciI CCGC 5 cut(s) 6, 16, 89, 168, 494
AclWI GGATC 1 cut(s) 348
AcoI YGGCCR 4 cut(s) 3, 86, 670, 676
AcsI RAATTY 2 cut(s) 764, 1629
AcuI CTGAAG 3 cut(s) 44, 178, 1590
AfaI GTAC 7 cut(s) 372, 587, 682, 1095, 1394, 1424, 1505
AfiI CCNNNNNNNGG 3 cut(s) 12, 1893, 2001
AgsI TTSAA 7 cut(s) 407, 659, 959, 1047, 1075, 1202, 1778
AjnI CCWGG 2 cut(s) 1056, 1533
AjuI GAANNNNNNNTTGG 2 cut(s) 1616, 1648
Alw21I GWGCWC 4 cut(s) 284, 649, 1696, 1868
Alw26I GTCTC 9 cut(s) 36, 87, 462, 900, 1008, 1327, 1365, 1558, 1766
Alw44I GTGCAC 1 cut(s) 1692
AlwI GGATC 1 cut(s) 348
Ama87I CYCGRG 2 cut(s) 477, 488
AoxI GGCC 9 cut(s) 3, 86, 128, 256, 550, 670, 676, 785, 1698
ApaI GGGCCC 1 cut(s) 132
ApaLI GTGCAC 1 cut(s) 1692
ApeKI GCWGC 8 cut(s) 189, 339, 395, 472, 720, 1739, 1742, 2033
ApoI RAATTY 2 cut(s) 764, 1629
ArsI GACNNNNNNTTYG 2 cut(s) 1046, 1078
AspLEI GCGC 4 cut(s) 224, 878, 1842, 1844
AspS9I GGNCC 7 cut(s) 128, 129, 481, 551, 785, 952, 1578
AsuC2I CCSGG 3 cut(s) 478, 479, 1837
AsuHPI GGTGA 5 cut(s) 443, 452, 525, 1556, 1910
AvaI CYCGRG 2 cut(s) 477, 488
AvaII GGWCC 3 cut(s) 481, 952, 1578
BaeGI GKGCMC 2 cut(s) 132, 1696
BalI TGGCCA 1 cut(s) 672
BanI GGYRCC 1 cut(s) 1184
BanII GRGCYC 2 cut(s) 132, 1868
BbsI GAAGAC 1 cut(s) 1241
Bbv12I GWGCWC 4 cut(s) 284, 649, 1696, 1868
BbvI GCAGC 7 cut(s) 201, 326, 382, 484, 732, 1751, 1754
BceAI ACGGC 2 cut(s) 663, 951
BcgI CGANNNNNNTGC 2 cut(s) 1948, 1982
BciT130I CCWGG 2 cut(s) 1058, 1535
BcnI CCSGG 3 cut(s) 478, 479, 1837
BcoDI GTCTC 9 cut(s) 36, 87, 462, 900, 1008, 1327, 1365, 1558, 1766
BfaI CTAG 5 cut(s) 987, 1452, 1514, 1685, 1893
BfmI CTRYAG 4 cut(s) 396, 561, 1584, 2031
BfoI RGCGCY 1 cut(s) 879
BglI GCCNNNNNGGC 1 cut(s) 1706
BglII AGATCT 2 cut(s) 400, 1510
BlpI GCTNAGC 2 cut(s) 497, 1782
BmcAI AGTACT 1 cut(s) 587
Bme1390I CCNGG 5 cut(s) 478, 479, 1058, 1535, 1837
Bme18I GGWCC 3 cut(s) 481, 952, 1578
BmeT110I CYCGRG 2 cut(s) 477, 488
BmgT120I GGNCC 7 cut(s) 128, 129, 481, 551, 785, 952, 1578
BmiI GGNNCC 8 cut(s) 130, 482, 553, 786, 1186, 1434, 1580, 1939
BmrFI CCNGG 5 cut(s) 478, 479, 1058, 1535, 1837
BmsI GCATC 7 cut(s) 417, 648, 652, 999, 1135, 1213, 1304
BpiI GAAGAC 1 cut(s) 1241
Bpu1102I GCTNAGC 2 cut(s) 497, 1782
BpuEI CTTGAG 3 cut(s) 734, 920, 1251
BpuMI CCSGG 3 cut(s) 478, 479, 1837
BsaAI YACGTR 1 cut(s) 12
BsaI GGTCTC 2 cut(s) 1558, 1766
BsaJI CCNNGG 4 cut(s) 476, 477, 673, 1995
BsaWI WCCGGW 1 cut(s) 979
BsaXI ACNNNNNCTCC 2 cut(s) 936, 966
Bsc4I CCNNNNNNNGG 3 cut(s) 12, 1893, 2001
Bse1I ACTGG 2 cut(s) 454, 1112
Bse3DI GCAATG 1 cut(s) 467
BseBI CCWGG 2 cut(s) 1058, 1535
BseDI CCNNGG 4 cut(s) 476, 477, 673, 1995
BseGI GGATG 8 cut(s) 68, 484, 524, 639, 993, 1495, 1762, 2010
BseLI CCNNNNNNNGG 3 cut(s) 12, 1893, 2001
BseMI GCAATG 1 cut(s) 467
BseMII CTCAG 8 cut(s) 26, 224, 267, 519, 1056, 1267, 1553, 1773
BseNI ACTGG 2 cut(s) 454, 1112
BsePI GCGCGC 1 cut(s) 1840
BseRI GAGGAG 8 cut(s) 90, 139, 142, 145, 148, 151, 154, 1418
BseSI GKGCMC 2 cut(s) 132, 1696
BseX3I CGGCCG 1 cut(s) 86
BseXI GCAGC 7 cut(s) 201, 326, 382, 484, 732, 1751, 1754
BseYI CCCAGC 1 cut(s) 1751
BsgI GTGCAG 1 cut(s) 1328
Bsh1236I CGCG 1 cut(s) 1842
Bsh1285I CGRYCG 1 cut(s) 89
BshFI GGCC 9 cut(s) 5, 88, 130, 258, 552, 672, 678, 787, 1700
BshNI GGYRCC 1 cut(s) 1184
BsiEI CGRYCG 1 cut(s) 89
BsiHKAI GWGCWC 4 cut(s) 284, 649, 1696, 1868
BsiHKCI CYCGRG 2 cut(s) 477, 488
BsiSI CCGG 3 cut(s) 478, 980, 1836
BslFI GGGAC 1 cut(s) 1564
BslI CCNNNNNNNGG 3 cut(s) 12, 1893, 2001
BsmAI GTCTC 9 cut(s) 36, 87, 462, 900, 1008, 1327, 1365, 1558, 1766
BsmBI CGTCTC 1 cut(s) 87
BsmFI GGGAC 1 cut(s) 1564
BsmI GAATGC 1 cut(s) 370
BsnI GGCC 9 cut(s) 5, 88, 130, 258, 552, 672, 678, 787, 1700
Bso31I GGTCTC 2 cut(s) 1558, 1766
BsoBI CYCGRG 2 cut(s) 477, 488
Bsp120I GGGCCC 1 cut(s) 128
Bsp1286I GDGCHC 5 cut(s) 132, 284, 649, 1696, 1868
Bsp1407I TGTACA 1 cut(s) 1503
Bsp143I GATC 5 cut(s) 353, 400, 1510, 1612, 1718
Bsp1720I GCTNAGC 2 cut(s) 497, 1782
Bsp19I CCATGG 2 cut(s) 673, 1995
BspACI CCGC 5 cut(s) 6, 16, 89, 168, 494
BspANI GGCC 9 cut(s) 5, 88, 130, 258, 552, 672, 678, 787, 1700
BspCNI CTCAG 8 cut(s) 27, 225, 268, 518, 1057, 1266, 1554, 1774
BspFNI CGCG 1 cut(s) 1842
BspLI GGNNCC 8 cut(s) 130, 482, 553, 786, 1186, 1434, 1580, 1939
BspMAI CTGCAG 3 cut(s) 400, 1588, 2035
BspPI GGATC 1 cut(s) 348
BspT107I GGYRCC 1 cut(s) 1184
BspTNI GGTCTC 2 cut(s) 1558, 1766
BsrDI GCAATG 1 cut(s) 467
BsrGI TGTACA 1 cut(s) 1503
BsrI ACTGG 2 cut(s) 454, 1112
BssECI CCNNGG 4 cut(s) 476, 477, 673, 1995
BssHII GCGCGC 1 cut(s) 1840
BssMI GATC 5 cut(s) 353, 400, 1510, 1612, 1718
BssT1I CCWWGG 2 cut(s) 673, 1995
Bst2UI CCWGG 2 cut(s) 1058, 1535
Bst4CI ACNGT 7 cut(s) 124, 562, 703, 859, 1098, 1285, 1966
Bst6I CTCTTC 3 cut(s) 145, 660, 1395
BstAUI TGTACA 1 cut(s) 1503
BstBAI YACGTR 1 cut(s) 12
BstC8I GCNNGC 4 cut(s) 741, 1086, 1161, 1842
BstDSI CCRYGG 2 cut(s) 673, 1995
BstEII GGTNACC 1 cut(s) 1990
BstF5I GGATG 8 cut(s) 68, 484, 524, 639, 993, 1495, 1762, 2010
BstFNI CGCG 1 cut(s) 1842
BstH2I RGCGCY 1 cut(s) 879
BstHHI GCGC 4 cut(s) 224, 878, 1842, 1844
BstKTI GATC 5 cut(s) 356, 403, 1513, 1615, 1721
BstMAI GTCTC 9 cut(s) 36, 87, 462, 900, 1008, 1327, 1365, 1558, 1766
BstMBI GATC 5 cut(s) 353, 400, 1510, 1612, 1718
BstMCI CGRYCG 1 cut(s) 89
BstMWI GCNNNNNNNGC 9 cut(s) 195, 228, 348, 729, 884, 1029, 1592, 1706, 1748
BstNI CCWGG 2 cut(s) 1058, 1535
BstNSI RCATGY 2 cut(s) 727, 1163
BstPI GGTNACC 1 cut(s) 1990
BstSCI CCNGG 5 cut(s) 476, 477, 1056, 1533, 1835
BstSFI CTRYAG 4 cut(s) 396, 561, 1584, 2031
BstSLI GKGCMC 2 cut(s) 132, 1696
BstUI CGCG 1 cut(s) 1842
BstV1I GCAGC 7 cut(s) 201, 326, 382, 484, 732, 1751, 1754
BstV2I GAAGAC 1 cut(s) 1241
BstX2I RGATCY 2 cut(s) 400, 1510
BstXI CCANNNNNNTGG 1 cut(s) 1124
BstYI RGATCY 2 cut(s) 400, 1510
BstZI CGGCCG 1 cut(s) 86
BsuRI GGCC 9 cut(s) 5, 88, 130, 258, 552, 672, 678, 787, 1700
BtgI CCRYGG 2 cut(s) 673, 1995
BtsCI GGATG 8 cut(s) 68, 484, 524, 639, 993, 1495, 1762, 2010
BtsI GCAGTG 2 cut(s) 270, 1602
BtsIMutI CAGTG 4 cut(s) 270, 1134, 1261, 1602
Cac8I GCNNGC 4 cut(s) 741, 1086, 1161, 1842
CfoI GCGC 4 cut(s) 224, 878, 1842, 1844
Cfr13I GGNCC 7 cut(s) 128, 129, 481, 551, 785, 952, 1578
Cfr9I CCCGGG 1 cut(s) 477
CsiI ACCWGGT 1 cut(s) 1056
Csp6I GTAC 7 cut(s) 371, 586, 681, 1094, 1393, 1423, 1504
CviQI GTAC 7 cut(s) 371, 586, 681, 1094, 1393, 1423, 1504
DpnI GATC 5 cut(s) 355, 402, 1512, 1614, 1720
DpnII GATC 5 cut(s) 353, 400, 1510, 1612, 1718
EaeI YGGCCR 4 cut(s) 3, 86, 670, 676
EagI CGGCCG 1 cut(s) 86
Eam1104I CTCTTC 3 cut(s) 145, 660, 1395
EarI CTCTTC 3 cut(s) 145, 660, 1395
EciI GGCGGA 2 cut(s) 31, 157
Ecl136II GAGCTC 1 cut(s) 1866
EclXI CGGCCG 1 cut(s) 86
Eco130I CCWWGG 2 cut(s) 673, 1995
Eco24I GRGCYC 2 cut(s) 132, 1868
Eco31I GGTCTC 2 cut(s) 1558, 1766
Eco47I GGWCC 3 cut(s) 481, 952, 1578
Eco52I CGGCCG 1 cut(s) 86
Eco53kI GAGCTC 1 cut(s) 1866
Eco57I CTGAAG 3 cut(s) 44, 178, 1590
Eco88I CYCGRG 2 cut(s) 477, 488
Eco91I GGTNACC 1 cut(s) 1990
EcoICRI GAGCTC 1 cut(s) 1866
EcoO109I RGGNCCY 3 cut(s) 129, 785, 952
EcoO65I GGTNACC 1 cut(s) 1990
EcoRII CCWGG 2 cut(s) 1056, 1533
EcoT14I CCWWGG 2 cut(s) 673, 1995
EcoT22I ATGCAT 2 cut(s) 432, 1161
EcoT38I GRGCYC 2 cut(s) 132, 1868
ErhI CCWWGG 2 cut(s) 673, 1995
Esp3I CGTCTC 1 cut(s) 87
FalI AAGNNNNNCTT 2 cut(s) 1770, 1802
FaqI GGGAC 1 cut(s) 1564
FauNDI CATATG 1 cut(s) 1338
FblI GTMKAC 1 cut(s) 441
FokI GGATG 8 cut(s) 55, 471, 511, 626, 980, 1502, 1769, 2017
FriOI GRGCYC 2 cut(s) 132, 1868
FspBI CTAG 5 cut(s) 987, 1452, 1514, 1685, 1893
GlaI GCGC 4 cut(s) 223, 877, 1841, 1843
GsaI CCCAGC 1 cut(s) 1755
HaeII RGCGCY 1 cut(s) 879
HaeIII GGCC 9 cut(s) 5, 88, 130, 258, 552, 672, 678, 787, 1700
HapII CCGG 3 cut(s) 478, 980, 1836
HhaI GCGC 4 cut(s) 224, 878, 1842, 1844
Hin6I GCGC 4 cut(s) 222, 876, 1840, 1842
HinP1I GCGC 4 cut(s) 222, 876, 1840, 1842
HincII GTYRAC 1 cut(s) 1378
HindII GTYRAC 1 cut(s) 1378
HindIII AAGCTT 2 cut(s) 737, 1572
HpaII CCGG 3 cut(s) 478, 980, 1836
HphI GGTGA 5 cut(s) 443, 452, 525, 1556, 1910
Hpy166II GTNNAC 5 cut(s) 442, 1117, 1378, 1423, 1694
Hpy188III TCNNGA 9 cut(s) 488, 571, 659, 707, 751, 937, 1268, 1685, 1921
Hpy8I GTNNAC 5 cut(s) 442, 1117, 1378, 1423, 1694
Hpy99I CGWCG 4 cut(s) 82, 128, 1970, 1987
HpyAV CCTTC 6 cut(s) 755, 1204, 1457, 1637, 1682, 1925
HpyCH4III ACNGT 7 cut(s) 124, 562, 703, 859, 1098, 1285, 1966
HpyCH4IV ACGT 5 cut(s) 11, 318, 711, 1380, 1714
HpyF10VI GCNNNNNNNGC 9 cut(s) 195, 228, 348, 729, 884, 1029, 1592, 1706, 1748
HpySE526I ACGT 5 cut(s) 11, 318, 711, 1380, 1714
HspAI GCGC 4 cut(s) 222, 876, 1840, 1842
Kzo9I GATC 5 cut(s) 353, 400, 1510, 1612, 1718
LmnI GCTCC 3 cut(s) 152, 287, 388
Lsp1109I GCAGC 7 cut(s) 201, 326, 382, 484, 732, 1751, 1754
LweI GCATC 7 cut(s) 417, 648, 652, 999, 1135, 1213, 1304
MabI ACCWGGT 1 cut(s) 1056
MaeI CTAG 5 cut(s) 987, 1452, 1514, 1685, 1893
MaeII ACGT 5 cut(s) 11, 318, 711, 1380, 1714
MaeIII GTNAC 6 cut(s) 38, 449, 697, 1898, 1990, 2007
MalI GATC 5 cut(s) 355, 402, 1512, 1614, 1720
MboI GATC 5 cut(s) 353, 400, 1510, 1612, 1718
MboII GAAGA 9 cut(s) 37, 132, 259, 395, 647, 861, 971, 1241, 1412
MfeI CAATTG 2 cut(s) 1018, 1079
MflI RGATCY 2 cut(s) 400, 1510
MhlI GDGCHC 5 cut(s) 132, 284, 649, 1696, 1868
MlsI TGGCCA 1 cut(s) 672
MluCI AATT 7 cut(s) 217, 764, 1018, 1079, 1412, 1629, 1768
MluNI TGGCCA 1 cut(s) 672
MlyI GAGTC 6 cut(s) 46, 537, 902, 927, 1272, 1367
MmeI TCCRAC 3 cut(s) 96, 1363, 1467
Mox20I TGGCCA 1 cut(s) 672
Mph1103I ATGCAT 2 cut(s) 432, 1161
MscI TGGCCA 1 cut(s) 672
MseI TTAA 4 cut(s) 53, 1529, 1730, 2025
MslI CAYNNNNRTG 4 cut(s) 435, 1154, 1341, 1873
Msp20I TGGCCA 1 cut(s) 672
MspI CCGG 3 cut(s) 478, 980, 1836
MspR9I CCNGG 5 cut(s) 478, 479, 1058, 1535, 1837
MunI CAATTG 2 cut(s) 1018, 1079
Mva1269I GAATGC 1 cut(s) 370
MvaI CCWGG 2 cut(s) 1058, 1535
MvnI CGCG 1 cut(s) 1842
MwoI GCNNNNNNNGC 9 cut(s) 195, 228, 348, 729, 884, 1029, 1592, 1706, 1748
NciI CCSGG 3 cut(s) 478, 479, 1837
NcoI CCATGG 2 cut(s) 673, 1995
NdeI CATATG 1 cut(s) 1338
NdeII GATC 5 cut(s) 353, 400, 1510, 1612, 1718
NlaIV GGNNCC 8 cut(s) 130, 482, 553, 786, 1186, 1434, 1580, 1939
NmeAIII GCCGAG 2 cut(s) 64, 95
NmuCI GTSAC 5 cut(s) 38, 449, 697, 1898, 2007
NsiI ATGCAT 2 cut(s) 432, 1161
NspI RCATGY 2 cut(s) 727, 1163
PaeI GCATGC 1 cut(s) 1163
PauI GCGCGC 1 cut(s) 1840
PcsI WCGNNNNNNNCGW 1 cut(s) 91
PctI GAATGC 1 cut(s) 370
PfeI GAWTC 8 cut(s) 65, 608, 976, 1172, 1202, 1820, 1915, 2014
PleI GAGTC 6 cut(s) 45, 537, 901, 927, 1272, 1366
PpsI GAGTC 6 cut(s) 45, 537, 901, 927, 1272, 1366
Ppu21I YACGTR 1 cut(s) 12
PpuMI RGGWCCY 1 cut(s) 952
Psp124BI GAGCTC 1 cut(s) 1868
Psp5II RGGWCCY 1 cut(s) 952
Psp6I CCWGG 2 cut(s) 1056, 1533
PspEI GGTNACC 1 cut(s) 1990
PspFI CCCAGC 1 cut(s) 1751
PspGI CCWGG 2 cut(s) 1056, 1533
PspN4I GGNNCC 8 cut(s) 130, 482, 553, 786, 1186, 1434, 1580, 1939
PspOMI GGGCCC 1 cut(s) 128
PspPI GGNCC 7 cut(s) 128, 129, 481, 551, 785, 952, 1578
PspPPI RGGWCCY 1 cut(s) 952
PstI CTGCAG 3 cut(s) 400, 1588, 2035
PsuI RGATCY 2 cut(s) 400, 1510
PteI GCGCGC 1 cut(s) 1840
RsaI GTAC 7 cut(s) 372, 587, 682, 1095, 1394, 1424, 1505
RsaNI GTAC 7 cut(s) 371, 586, 681, 1094, 1393, 1423, 1504
RseI CAYNNNNRTG 4 cut(s) 435, 1154, 1341, 1873
SacI GAGCTC 1 cut(s) 1868
SaqAI TTAA 4 cut(s) 53, 1529, 1730, 2025
Sau3AI GATC 5 cut(s) 353, 400, 1510, 1612, 1718
Sau96I GGNCC 7 cut(s) 128, 129, 481, 551, 785, 952, 1578
ScaI AGTACT 1 cut(s) 587
SchI GAGTC 6 cut(s) 46, 537, 902, 927, 1272, 1367
ScrFI CCNGG 5 cut(s) 478, 479, 1058, 1535, 1837
SduI GDGCHC 5 cut(s) 132, 284, 649, 1696, 1868
SexAI ACCWGGT 1 cut(s) 1056
SfaNI GCATC 7 cut(s) 417, 648, 652, 999, 1135, 1213, 1304
SfcI CTRYAG 4 cut(s) 396, 561, 1584, 2031
SinI GGWCC 3 cut(s) 481, 952, 1578
SmaI CCCGGG 1 cut(s) 479
SmiMI CAYNNNNRTG 4 cut(s) 435, 1154, 1341, 1873
SmlI CTYRAG 3 cut(s) 749, 935, 1266
SmoI CTYRAG 3 cut(s) 749, 935, 1266
SphI GCATGC 1 cut(s) 1163
Sse9I AATT 7 cut(s) 217, 764, 1018, 1079, 1412, 1629, 1768
SsiI CCGC 5 cut(s) 6, 16, 89, 168, 494
SspI AATATT 1 cut(s) 1108
SspMI CTAG 5 cut(s) 987, 1452, 1514, 1685, 1893
SstI GAGCTC 1 cut(s) 1868
StyD4I CCNGG 5 cut(s) 476, 477, 1056, 1533, 1835
StyI CCWWGG 2 cut(s) 673, 1995
TaaI ACNGT 7 cut(s) 124, 562, 703, 859, 1098, 1285, 1966
TaiI ACGT 5 cut(s) 14, 321, 714, 1383, 1717
TaqI TCGA 3 cut(s) 541, 891, 1276
TaqII GACCGA 1 cut(s) 810
TasI AATT 7 cut(s) 217, 764, 1018, 1079, 1412, 1629, 1768
TatI WGTACW 2 cut(s) 585, 1503
TauI GCSGC 3 cut(s) 8, 91, 496
TfiI GAWTC 8 cut(s) 65, 608, 976, 1172, 1202, 1820, 1915, 2014
Tru1I TTAA 4 cut(s) 53, 1529, 1730, 2025
Tru9I TTAA 4 cut(s) 53, 1529, 1730, 2025
TscAI CASTG 4 cut(s) 277, 1141, 1261, 1602
TseFI GTSAC 5 cut(s) 38, 449, 697, 1898, 2007
TseI GCWGC 8 cut(s) 189, 339, 395, 472, 720, 1739, 1742, 2033
Tsp45I GTSAC 5 cut(s) 38, 449, 697, 1898, 2007
TspDTI ATGAA 6 cut(s) 57, 528, 1164, 1186, 1863, 1989
TspMI CCCGGG 1 cut(s) 477
TspRI CASTG 4 cut(s) 277, 1141, 1261, 1602
VneI GTGCAC 1 cut(s) 1692
VpaK11BI GGWCC 3 cut(s) 481, 952, 1578
XapI RAATTY 2 cut(s) 764, 1629
XbaI TCTAGA 1 cut(s) 1684
XceI RCATGY 2 cut(s) 727, 1163
XmaI CCCGGG 1 cut(s) 477
XmiI GTMKAC 1 cut(s) 441
XspI CTAG 5 cut(s) 987, 1452, 1514, 1685, 1893
ZrmI AGTACT 1 cut(s) 587
Zsp2I ATGCAT 2 cut(s) 432, 1161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.