Rh5BG138100

VWA / Hh protein intein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000360
Physical Location & Seq
Forward (+)
14193827 .. 14196634
2808 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG138100.1

Sequence Viewer

Length: 1917 bp
ATGTCGTCTCAGGATGCATCTGCTCAAAAAAATTGCGCAATCTGCTGGAGTAACGTGACAACAGGGCAAGGCCAAGCCATCTTCACTGCTGAGTGCTCACACTCTTTCCACTACCCTTGCATTGCCAACAATGTCCAGCATGGAAATCTTTGCTGCCCCATCTGCCGAGCAAAATGGGATAAGAATAATGTTCCTTTCCAAGTTCCCCCGCTTCAACAAAACAACTTGGGTGCACATGGCTTCGCTGATGATGAACCTCTCCCGTTCACCTCACCTGCTCAATCTTCTGGCCCCCAGAATGTCACAATCAAAACTCATACAGAGACCTCTGCTATCCCTGCTGCCGATTCACGCCCACAATATCCTGTTCTAGTCAGCATCTGTGCACCACCCCTTCAGGATCCTGATGGCGAAGTCCGTACACCGATTGACCTTGTAACAGTTCTAGACGTAAGTGGCAGCATGCAGGGCCAAAAGCTTGACCTTGTCAAGCAAGCTATCAAATTTGTCATAGAAAACATGGGGTCTTCAGATAGACTTTCTATAGTTTCATTCTCAACAAATTCTAGAAGAGTCCTTCCTCTTCGAAGAATGACAGTTGATGGCCGTGAAAGTGCAATTGAAGCTGTCGAATCTCTTGTAGCAGGTGGTGGAACTGACATTGCAGAAGGACTCAAAAAAGGAACACAAGTCCTTGAAGATCGGAGGCAAAGGAACCCAGTTGCTAGCATCATTCTCTTATCCGATGGCCAAGATAACTACTGTCGAAGCCCAAGCCAAATGTTGAAGAAATTGCCTGCTTCAATTCGTTCCAGCGACATGCAACATGAAATCCCTGTCCACACATTTGGGTTCGGCAACGACCATGATGCAAAGATTATGCATGCTATATCTGAGGAATCCCGGGGCACCTTTTCATACATTGAATCAGTTGGCATGATACAAGACGCCTTTGCTCTGTGCATTGGTGGTCTTCTCAGTGTTGTGGCTCAGGAACTTCGCCTCACAGTGAGGTCAGCATCACATGGAGCGAAGATTGTATCAATACCTTCAGGGAGATATGTGAGTCAGATTTCTGATGACGGCTCGCAAGGTTTAGTTGATATTGGAAATATGTACGCAGAAGAGGTGAAACAATTTCTGGTGTATCTCTTAGTTCCACAATCCTCAGCTTCAGAGACTAAGACATCACTGATGGACGTCTTATGCGTGTACAGAGATCTAGCTTCAAATGAGTTTATCCAGCTCCAAAGTGAGAGAGTAGAGATATCAAGACCTGAGTTTTGTTCTCCTACAGAATTGGCTGTGTGTTTGGAGGTTGATCGCCAGCGGAACAGACTTTTGGTGGCTGATGCTATTGGAGAGGCTCAACGCTTGGCTGAAATGGGAAATTTGGAGGGTGCACGCGAAATTTTGACTCAACGAAGAGAAACTCTCTTAGCATCAGCAGCAGCCAAAGCTGGGGATAGTCTCAGTAATATGTTTGAAACTGAGCTTAAAGAAATCAGGGATAAAATGGCAAGTATGAAGTTGTACGAAGAGGCGGGGCGTGCTTATGCTCTGGCTGGAATGAGTTCACATTCACGCCAGAGGGCCTCTGCTAGGGGTAACGCAGAAGGCTCGTGCCTGGCCATGAAGGGTCGCAAACAACTTGCTACAAAAGCAGCGGGTCGTGCTTCCGGGGGTCGCAGTGGAGCAGCGACTCTTGCTGCCGGGGGTTTTGGTGGCAAGGGTGGTCCCAAAGGCAAACGCTCTGCAATGGATCACTTAGTGGCTGCTGCGGGAGACTCTGATGAAGATGATGATATGAGCTTTGCTTTATTTGACGGAGGCAATGATGTCCATCGAGGTGCTTTTGAAACACCAGCAATGGTAAGAATGGTACAGAAATCACAGCAGAAGGATCAATCTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VWA Vwaint zf-RING_11 zf-RING_2

Protein Analysis

638

Amino Acids

68.66

Weight (kDa)

5.97

Isoelectric Point (pI)

51.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_11 PF17123 11 - 41 6e-10 RING-like zinc finger
zf-RING_2 PF13639 11 - 56 2e-06 Ring finger domain
VWA_CoxE PF05762 140 - 258 6.4e-08 VWA domain containing CoxE-like protein
VWA PF00092 144 - 312 1.3e-25 von Willebrand factor type A domain
VWA_3 PF13768 144 - 305 2.1e-20 von Willebrand factor type A domain
VWA_2 PF13519 145 - 251 2.3e-19 von Willebrand factor type A domain
WAV3_C PF25243 440 - 506 1e-05 E3 ubiquitin-protein ligase WAV3-like, C-terminal domain
Vwaint PF14624 495 - 537 9.5e-10 VWA / Hh protein intein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000360)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11691 FvH4_3g11693 FvH4_3g11740 FvH4_3g11750 FvH4_3g11770 FvH4_3g11771 FvH4_3g11772 FvH4_3g11780
malus_domestica MD10G1239000.v1.1
prunus_persica Prupe.4G105400_v2.0.a1
pyrus_communis pycom10g20000
rosa_chinensis RchiOBHm_Chr5g0018981 RchiOBHm_Chr5g0018991 RchiOBHm_Chr5g0019101 RchiOBHm_Chr5g0019111 RchiOBHm_Chr5g0019121 RchiOBHm_Chr5g0019151 RchiOBHm_Chr5g0019161 RchiOBHm_Chr5g0019171 RchiOBHm_Chr5g0019181 RchiOBHm_Chr5g0019201
rosa_laevigata RLG00000032411 RLG00000032418 RLG00000032419 RLG00000032420 RLG00000032421 RLG00000032423 RLG00000032424 RLG00000032426
rosa_multiflora Rmu_co8475613.1_g000001 Rmu_co8503587.1_g000001 Rmu_sc0000563.1_g000023 Rmu_sc0000563.1_g000027 Rmu_sc0002142.1_g000001 Rmu_sc0002142.1_g000003 Rmu_sc0002142.1_g000004 Rmu_sc0002142.1_g000011 Rmu_sc0002142.1_g000022 Rmu_sc0002561.1_g000002 Rmu_sc0042836.1_g000001
rosa_roxburghii Rroxscaffold_1G00058790 Rroxscaffold_1G00058810 Rroxscaffold_1G00058820 Rroxscaffold_1G00058840 Rroxscaffold_1G00058850 Rroxscaffold_1G00058860 Rroxscaffold_1G00058880 Rroxscaffold_1G00058910 Rroxscaffold_1G00074090 Rroxscaffold_1G00074110 Rroxscaffold_1G00074120 Rroxscaffold_1G00074130 Rroxscaffold_1G00074150
rosa_rugosa Rorug05G0046800 Rorug05G0047200 Rorug05G0047300 Rorug05G0047400 Rorug05G0047600 Rorug05G0047600 Rorug05G0047800 Rorug05G0047900 Rorug05G0048000 Rorug05G0048100 Rorug05G0048100 Rorug05G0048200 Rorug05G0048300
rosa_samantha Rh4DG102800 Rh5AG138700 Rh5AG138900 Rh5AG139000 Rh5AG139100 Rh5AG139200 Rh5AG139400 Rh5AG139500 Rh5AG139600 Rh5BG137400 Rh5BG137600 Rh5BG137800 Rh5BG138100 Rh5BG138200 Rh5BG138300 Rh5BG138500 Rh5CG148800 Rh5CG148900 Rh5CG149200 Rh5CG149300 Rh5CG149400 Rh5CG149600 Rh5CG149700 Rh5CG150000 Rh5DG138200 Rh5DG138300 Rh5DG138500 Rh5DG138600 Rh5DG138700 Rh5DG138900 Rh5DG139000 Rh5DG139200
rosa_wichuraiana Rw5G012290 Rw5G012330 Rw5G012340 Rw5G012350 Rw5G012370 Rw5G012380 Rw5G012390 Rw5G012410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 283, 635
AatII GACGTC 1 cut(s) 1203
Acc16I TGCGCA 1 cut(s) 37
Acc36I ACCTGC 2 cut(s) 283, 635
AccB1I GGYRCC 1 cut(s) 908
AccII CGCG 1 cut(s) 1407
AciI CCGC 5 cut(s) 209, 1330, 1544, 1667, 1781
AclWI GGATC 4 cut(s) 395, 408, 1770, 1911
AcoI YGGCCR 3 cut(s) 604, 748, 1629
AcsI RAATTY 4 cut(s) 503, 562, 1390, 1410
AcuI CTGAAG 4 cut(s) 380, 513, 1035, 1158
AcyI GRCGYC 2 cut(s) 948, 1200
AdeI CACNNNGTG 1 cut(s) 1771
AfaI GTAC 5 cut(s) 421, 1118, 1214, 1535, 1884
AfiI CCNNNNNNNGG 2 cut(s) 1461, 1602
AgsI TTSAA 9 cut(s) 215, 623, 698, 787, 804, 926, 1230, 1487, 1859
AjnI CCWGG 1 cut(s) 1626
AjuI GAANNNNNNNTTGG 2 cut(s) 1325, 1357
AluBI AGCT 9 cut(s) 478, 497, 626, 1172, 1226, 1246, 1460, 1495, 1812
AluI AGCT 9 cut(s) 478, 497, 626, 1172, 1226, 1246, 1460, 1495, 1812
Alw21I GWGCWC 4 cut(s) 98, 235, 388, 1405
Alw26I GTCTC 5 cut(s) 12, 317, 1172, 1475, 1779
Alw44I GTGCAC 3 cut(s) 231, 384, 1401
AlwI GGATC 4 cut(s) 395, 408, 1770, 1911
AlwNI CAGNNNCTG 1 cut(s) 381
Ama87I CYCGRG 1 cut(s) 903
AoxI GGCC 7 cut(s) 70, 289, 469, 604, 748, 1593, 1629
ApaLI GTGCAC 3 cut(s) 231, 384, 1401
ApoI RAATTY 4 cut(s) 503, 562, 1390, 1410
ArsI GACNNNNNNTTYG 2 cut(s) 1266, 1298
Asp700I GAANNNNTTC 1 cut(s) 1573
AspLEI GCGC 1 cut(s) 38
AspS9I GGNCC 4 cut(s) 290, 469, 1593, 1736
AsuC2I CCSGG 4 cut(s) 904, 905, 1681, 1714
AsuHPI GGTGA 3 cut(s) 259, 264, 1141
AsuII TTCGAA 1 cut(s) 586
AsuNHI GCTAGC 1 cut(s) 725
AvaI CYCGRG 1 cut(s) 903
AvaII GGWCC 1 cut(s) 1736
BaeGI GKGCMC 4 cut(s) 235, 388, 911, 1405
BalI TGGCCA 2 cut(s) 750, 1631
BamHI GGATCC 1 cut(s) 400
BanI GGYRCC 1 cut(s) 908
BauI CACGAG 1 cut(s) 1621
BbsI GAAGAC 2 cut(s) 519, 965
Bbv12I GWGCWC 4 cut(s) 98, 235, 388, 1405
BbvCI CCTCAGC 1 cut(s) 1168
BccI CCATC 7 cut(s) 86, 167, 401, 596, 740, 1189, 1851
BceAI ACGGC 2 cut(s) 591, 1099
BcgI CGANNNNNNTGC 4 cut(s) 851, 885, 1602, 1636
BciT130I CCWGG 1 cut(s) 1628
BcnI CCSGG 4 cut(s) 904, 905, 1681, 1714
BcoDI GTCTC 5 cut(s) 12, 317, 1172, 1475, 1779
BfaI CTAG 6 cut(s) 371, 446, 567, 726, 1223, 1602
BfmI CTRYAG 2 cut(s) 543, 1293
BfuAI ACCTGC 2 cut(s) 283, 635
BglII AGATCT 1 cut(s) 1219
Bme1390I CCNGG 5 cut(s) 904, 905, 1628, 1681, 1714
Bme18I GGWCC 1 cut(s) 1736
BmeT110I CYCGRG 1 cut(s) 903
BmgT120I GGNCC 4 cut(s) 290, 469, 1593, 1736
BmiI GGNNCC 5 cut(s) 292, 402, 716, 910, 1738
BmrFI CCNGG 5 cut(s) 904, 905, 1628, 1681, 1714
BmrI ACTGGG 1 cut(s) 713
BmsI GCATC 8 cut(s) 4, 26, 387, 738, 859, 1028, 1342, 1451
BmtI GCTAGC 1 cut(s) 729
BmuI ACTGGG 1 cut(s) 713
BpiI GAAGAC 2 cut(s) 519, 965
BplI GAGNNNNNCTC 2 cut(s) 1419, 1451
BpmI CTGGAG 1 cut(s) 67
Bpu10I CCTNAGC 2 cut(s) 990, 1168
Bpu14I TTCGAA 1 cut(s) 586
BpuMI CCSGG 4 cut(s) 904, 905, 1681, 1714
BsaBI GATNNNNATC 1 cut(s) 1842
BsaHI GRCGYC 2 cut(s) 948, 1200
BsaI GGTCTC 1 cut(s) 317
BsaJI CCNNGG 4 cut(s) 903, 904, 1680, 1713
Bsc4I CCNNNNNNNGG 2 cut(s) 1461, 1602
Bse1I ACTGG 1 cut(s) 719
Bse3DI GCAATG 5 cut(s) 120, 660, 1764, 1840, 1875
Bse8I GATNNNNATC 1 cut(s) 1842
BseBI CCWGG 1 cut(s) 1628
BseDI CCNNGG 4 cut(s) 903, 904, 1680, 1713
BseGI GGATG 1 cut(s) 19
BseJI GATNNNNATC 1 cut(s) 1842
BseLI CCNNNNNNNGG 2 cut(s) 1461, 1602
BseMI GCAATG 5 cut(s) 120, 660, 1764, 1840, 1875
BseMII CTCAG 9 cut(s) 23, 81, 885, 991, 1004, 1182, 1269, 1482, 1486
BseNI ACTGG 1 cut(s) 719
BseSI GKGCMC 4 cut(s) 235, 388, 911, 1405
BseYI CCCAGC 1 cut(s) 1460
Bsh1236I CGCG 1 cut(s) 1407
BshFI GGCC 7 cut(s) 72, 291, 471, 606, 750, 1595, 1631
BshNI GGYRCC 1 cut(s) 908
BsiHKAI GWGCWC 4 cut(s) 98, 235, 388, 1405
BsiHKCI CYCGRG 1 cut(s) 903
BsiSI CCGG 3 cut(s) 904, 1680, 1713
BslFI GGGAC 1 cut(s) 1722
BslI CCNNNNNNNGG 2 cut(s) 1461, 1602
BsmAI GTCTC 5 cut(s) 12, 317, 1172, 1475, 1779
BsmBI CGTCTC 1 cut(s) 12
BsmFI GGGAC 1 cut(s) 1722
BsnI GGCC 7 cut(s) 72, 291, 471, 606, 750, 1595, 1631
Bso31I GGTCTC 1 cut(s) 317
BsoBI CYCGRG 1 cut(s) 903
Bsp119I TTCGAA 1 cut(s) 586
Bsp1286I GDGCHC 5 cut(s) 98, 235, 388, 911, 1405
Bsp1407I TGTACA 1 cut(s) 1212
Bsp143I GATC 6 cut(s) 400, 700, 1219, 1321, 1762, 1903
BspACI CCGC 5 cut(s) 209, 1330, 1544, 1667, 1781
BspANI GGCC 7 cut(s) 72, 291, 471, 606, 750, 1595, 1631
BspCNI CTCAG 9 cut(s) 22, 82, 886, 990, 1003, 1181, 1270, 1483, 1485
BspFNI CGCG 1 cut(s) 1407
BspLI GGNNCC 5 cut(s) 292, 402, 716, 910, 1738
BspMI ACCTGC 2 cut(s) 283, 635
BspOI GCTAGC 1 cut(s) 729
BspPI GGATC 4 cut(s) 395, 408, 1770, 1911
BspT104I TTCGAA 1 cut(s) 586
BspT107I GGYRCC 1 cut(s) 908
BspTNI GGTCTC 1 cut(s) 317
BsrDI GCAATG 5 cut(s) 120, 660, 1764, 1840, 1875
BsrGI TGTACA 1 cut(s) 1212
BsrI ACTGG 1 cut(s) 719
BssECI CCNNGG 4 cut(s) 903, 904, 1680, 1713
BssMI GATC 6 cut(s) 400, 700, 1219, 1321, 1762, 1903
BssNI GRCGYC 2 cut(s) 948, 1200
BssSI CACGAG 1 cut(s) 1621
Bst2BI CACGAG 1 cut(s) 1621
Bst2UI CCWGG 1 cut(s) 1628
Bst4CI ACNGT 4 cut(s) 442, 598, 764, 1009
Bst6I CTCTTC 5 cut(s) 565, 588, 1119, 1420, 1533
BstACI GRCGYC 2 cut(s) 948, 1200
BstAUI TGTACA 1 cut(s) 1212
BstBI TTCGAA 1 cut(s) 586
BstC8I GCNNGC 9 cut(s) 464, 495, 727, 798, 885, 1088, 1328, 1405, 1551
BstENI CCTNNNNNAGG 1 cut(s) 1600
BstF5I GGATG 1 cut(s) 19
BstFNI CGCG 1 cut(s) 1407
BstHHI GCGC 1 cut(s) 38
BstKTI GATC 6 cut(s) 403, 703, 1222, 1324, 1765, 1906
BstMAI GTCTC 5 cut(s) 12, 317, 1172, 1475, 1779
BstMBI GATC 6 cut(s) 400, 700, 1219, 1321, 1762, 1903
BstNI CCWGG 1 cut(s) 1628
BstNSI RCATGY 3 cut(s) 466, 823, 887
BstSCI CCNGG 5 cut(s) 902, 903, 1626, 1679, 1712
BstSFI CTRYAG 2 cut(s) 543, 1293
BstSLI GKGCMC 4 cut(s) 235, 388, 911, 1405
BstUI CGCG 1 cut(s) 1407
BstV2I GAAGAC 2 cut(s) 519, 965
BstX2I RGATCY 2 cut(s) 400, 1219
BstXI CCANNNNNNTGG 1 cut(s) 848
BstYI RGATCY 2 cut(s) 400, 1219
BsuRI GGCC 7 cut(s) 72, 291, 471, 606, 750, 1595, 1631
BtsCI GGATG 1 cut(s) 19
BtsI GCAGTG 2 cut(s) 84, 1696
BtsIMutI CAGTG 5 cut(s) 84, 985, 1014, 1190, 1696
BveI ACCTGC 2 cut(s) 283, 635
Cac8I GCNNGC 9 cut(s) 464, 495, 727, 798, 885, 1088, 1328, 1405, 1551
CaiI CAGNNNCTG 1 cut(s) 381
CfoI GCGC 1 cut(s) 38
Cfr13I GGNCC 4 cut(s) 290, 469, 1593, 1736
Cfr9I CCCGGG 1 cut(s) 903
CseI GACGC 1 cut(s) 956
Csp6I GTAC 5 cut(s) 420, 1117, 1213, 1534, 1883
CspCI CAANNNNNGTGG 2 cut(s) 98, 133
CviQI GTAC 5 cut(s) 420, 1117, 1213, 1534, 1883
DpnI GATC 6 cut(s) 402, 702, 1221, 1323, 1764, 1905
DpnII GATC 6 cut(s) 400, 700, 1219, 1321, 1762, 1903
DraIII CACNNNGTG 1 cut(s) 1771
EaeI YGGCCR 3 cut(s) 604, 748, 1629
Eam1104I CTCTTC 5 cut(s) 565, 588, 1119, 1420, 1533
EarI CTCTTC 5 cut(s) 565, 588, 1119, 1420, 1533
Eco31I GGTCTC 1 cut(s) 317
Eco32I GATATC 1 cut(s) 1269
Eco47I GGWCC 1 cut(s) 1736
Eco57I CTGAAG 4 cut(s) 380, 513, 1035, 1158
Eco88I CYCGRG 1 cut(s) 903
EcoNI CCTNNNNNAGG 1 cut(s) 1600
EcoO109I RGGNCCY 1 cut(s) 1593
EcoRII CCWGG 1 cut(s) 1626
EcoRV GATATC 1 cut(s) 1269
EcoT22I ATGCAT 2 cut(s) 19, 885
Esp3I CGTCTC 1 cut(s) 12
FaqI GGGAC 1 cut(s) 1722
FauI CCCGC 4 cut(s) 216, 1537, 1660, 1774
FokI GGATG 1 cut(s) 26
FspBI CTAG 6 cut(s) 371, 446, 567, 726, 1223, 1602
FspI TGCGCA 1 cut(s) 37
GlaI GCGC 1 cut(s) 37
GsaI CCCAGC 1 cut(s) 1464
GsuI CTGGAG 1 cut(s) 67
HaeIII GGCC 7 cut(s) 72, 291, 471, 606, 750, 1595, 1631
HapII CCGG 3 cut(s) 904, 1680, 1713
HgaI GACGC 1 cut(s) 956
HhaI GCGC 1 cut(s) 38
Hin1I GRCGYC 2 cut(s) 948, 1200
Hin6I GCGC 1 cut(s) 36
HinP1I GCGC 1 cut(s) 36
HindIII AAGCTT 1 cut(s) 476
HpaII CCGG 3 cut(s) 904, 1680, 1713
HphI GGTGA 3 cut(s) 259, 264, 1141
Hpy166II GTNNAC 8 cut(s) 233, 267, 386, 422, 841, 1213, 1403, 1577
Hpy188I TCNGA 8 cut(s) 532, 705, 745, 895, 1071, 1078, 1177, 1792
Hpy188III TCNNGA 7 cut(s) 11, 398, 404, 446, 567, 992, 1272
Hpy8I GTNNAC 8 cut(s) 233, 267, 386, 422, 841, 1213, 1403, 1577
HpyAV CCTTC 7 cut(s) 404, 587, 662, 1059, 1610, 1630, 1894
HpyCH4III ACNGT 4 cut(s) 442, 598, 764, 1009
HpyCH4IV ACGT 3 cut(s) 54, 450, 1200
HpySE526I ACGT 3 cut(s) 54, 450, 1200
Hsp92I GRCGYC 2 cut(s) 948, 1200
HspAI GCGC 1 cut(s) 36
Kzo9I GATC 6 cut(s) 400, 700, 1219, 1321, 1762, 1903
LmnI GCTCC 3 cut(s) 1028, 1251, 1694
LweI GCATC 8 cut(s) 4, 26, 387, 738, 859, 1028, 1342, 1451
MaeI CTAG 6 cut(s) 371, 446, 567, 726, 1223, 1602
MaeII ACGT 3 cut(s) 54, 450, 1200
MaeIII GTNAC 5 cut(s) 50, 55, 301, 436, 1607
MalI GATC 6 cut(s) 402, 702, 1221, 1323, 1764, 1905
MboI GATC 6 cut(s) 400, 700, 1219, 1321, 1762, 1903
MfeI CAATTG 1 cut(s) 618
MflI RGATCY 2 cut(s) 400, 1219
MhlI GDGCHC 5 cut(s) 98, 235, 388, 911, 1405
MlsI TGGCCA 2 cut(s) 750, 1631
MluNI TGGCCA 2 cut(s) 750, 1631
MlyI GAGTC 6 cut(s) 582, 666, 1075, 1411, 1696, 1781
Mox20I TGGCCA 2 cut(s) 750, 1631
Mph1103I ATGCAT 2 cut(s) 19, 885
MroXI GAANNNNTTC 1 cut(s) 1573
MscI TGGCCA 2 cut(s) 750, 1631
MseI TTAA 1 cut(s) 1497
MslI CAYNNNNRTG 1 cut(s) 1848
Msp20I TGGCCA 2 cut(s) 750, 1631
MspA1I CMGCKG 2 cut(s) 1330, 1667
MspI CCGG 3 cut(s) 904, 1680, 1713
MspR9I CCNGG 5 cut(s) 904, 905, 1628, 1681, 1714
MunI CAATTG 1 cut(s) 618
MvaI CCWGG 1 cut(s) 1628
MvnI CGCG 1 cut(s) 1407
NciI CCSGG 4 cut(s) 904, 905, 1681, 1714
NdeII GATC 6 cut(s) 400, 700, 1219, 1321, 1762, 1903
NheI GCTAGC 1 cut(s) 725
NlaIV GGNNCC 5 cut(s) 292, 402, 716, 910, 1738
NmeAIII GCCGAG 1 cut(s) 191
NmuCI GTSAC 2 cut(s) 55, 301
NsbI TGCGCA 1 cut(s) 37
NsiI ATGCAT 2 cut(s) 19, 885
NspI RCATGY 3 cut(s) 466, 823, 887
NspV TTCGAA 1 cut(s) 586
PaeI GCATGC 2 cut(s) 466, 887
PaqCI CACCTGC 2 cut(s) 283, 635
PcsI WCGNNNNNNNCGW 1 cut(s) 1206
PdmI GAANNNNTTC 1 cut(s) 1573
PfeI GAWTC 4 cut(s) 347, 632, 899, 926
PflFI GACNNNGTC 1 cut(s) 485
PleI GAGTC 6 cut(s) 581, 666, 1074, 1411, 1696, 1781
PpsI GAGTC 6 cut(s) 581, 666, 1074, 1411, 1696, 1781
Psp6I CCWGG 1 cut(s) 1626
PspFI CCCAGC 1 cut(s) 1460
PspGI CCWGG 1 cut(s) 1626
PspN4I GGNNCC 5 cut(s) 292, 402, 716, 910, 1738
PspPI GGNCC 4 cut(s) 290, 469, 1593, 1736
PstNI CAGNNNCTG 1 cut(s) 381
PsuI RGATCY 2 cut(s) 400, 1219
PsyI GACNNNGTC 1 cut(s) 485
RsaI GTAC 5 cut(s) 421, 1118, 1214, 1535, 1884
RsaNI GTAC 5 cut(s) 420, 1117, 1213, 1534, 1883
RseI CAYNNNNRTG 1 cut(s) 1848
SaqAI TTAA 1 cut(s) 1497
Sau3AI GATC 6 cut(s) 400, 700, 1219, 1321, 1762, 1903
Sau96I GGNCC 4 cut(s) 290, 469, 1593, 1736
SchI GAGTC 6 cut(s) 582, 666, 1075, 1411, 1696, 1781
ScrFI CCNGG 5 cut(s) 904, 905, 1628, 1681, 1714
SduI GDGCHC 5 cut(s) 98, 235, 388, 911, 1405
SfaNI GCATC 8 cut(s) 4, 26, 387, 738, 859, 1028, 1342, 1451
SfcI CTRYAG 2 cut(s) 543, 1293
SfuI TTCGAA 1 cut(s) 586
SinI GGWCC 1 cut(s) 1736
SmaI CCCGGG 1 cut(s) 905
SmiMI CAYNNNNRTG 1 cut(s) 1848
SphI GCATGC 2 cut(s) 466, 887
SsiI CCGC 5 cut(s) 209, 1330, 1544, 1667, 1781
SspMI CTAG 6 cut(s) 371, 446, 567, 726, 1223, 1602
StyD4I CCNGG 5 cut(s) 902, 903, 1626, 1679, 1712
TaaI ACNGT 4 cut(s) 442, 598, 764, 1009
TaiI ACGT 3 cut(s) 57, 453, 1203
TaqI TCGA 4 cut(s) 586, 630, 766, 1846
TatI WGTACW 1 cut(s) 1212
TfiI GAWTC 4 cut(s) 347, 632, 899, 926
Tru1I TTAA 1 cut(s) 1497
Tru9I TTAA 1 cut(s) 1497
TscAI CASTG 5 cut(s) 91, 985, 1014, 1197, 1696
TseFI GTSAC 2 cut(s) 55, 301
Tsp45I GTSAC 2 cut(s) 55, 301
TspDTI ATGAA 7 cut(s) 267, 540, 843, 906, 1541, 1649, 1809
TspGWI ACGGA 2 cut(s) 407, 1842
TspMI CCCGGG 1 cut(s) 903
TspRI CASTG 5 cut(s) 91, 985, 1014, 1197, 1696
Tth111I GACNNNGTC 1 cut(s) 485
VneI GTGCAC 3 cut(s) 231, 384, 1401
VpaK11BI GGWCC 1 cut(s) 1736
XagI CCTNNNNNAGG 1 cut(s) 1600
XapI RAATTY 4 cut(s) 503, 562, 1390, 1410
XbaI TCTAGA 2 cut(s) 445, 566
XceI RCATGY 3 cut(s) 466, 823, 887
XmaI CCCGGG 1 cut(s) 903
XmnI GAANNNNTTC 1 cut(s) 1573
XspI CTAG 6 cut(s) 371, 446, 567, 726, 1223, 1602
ZraI GACGTC 1 cut(s) 1201
Zsp2I ATGCAT 2 cut(s) 19, 885
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.